Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,506,318 (C)5→6 coding (1134/1227 nt) yhfW ← putative mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,506,3131.C88.8% 778.4 / 90.5 259coding (1139/1227 nt)yhfWputative mutase
Reads supporting (aligned to +/- strand):  ref base . (11/18);  new base C (122/108);  total (133/126)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-02

Only 100 of 282 total aligned reads displayed.

AAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGG  >  NC_000913/3506221‑3506405
                                                                                             |                                                                                            
aaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAg                                                                                               >  2:3109582/1‑93 (MQ=255)
  aCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑cc                                                                                            <  1:5273433/93‑1 (MQ=255)
  aCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑cc                                                                                            <  1:3348926/93‑1 (MQ=255)
   cTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑ccc                                                                                           <  2:6130636/93‑1 (MQ=255)
    tCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑cccc                                                                                          >  1:7294986/1‑93 (MQ=255)
    tCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑cccc                                                                                          >  2:3540505/1‑93 (MQ=255)
      aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑cccccc                                                                                        <  2:705145/93‑2 (MQ=255)
        aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑ccccccac                                                                                      >  1:5602345/1‑90 (MQ=255)
         gggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑ccccccaca                                                                                     >  1:3125587/1‑89 (MQ=255)
           gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATc                                                                                    <  2:5023521/93‑1 (MQ=255)
           gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCa                                                                                   <  2:361096/93‑1 (MQ=255)
           gtgtCTACTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATc                                                                                    <  1:1442912/93‑1 (MQ=255)
             gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAg                                                                                  >  2:6687251/1‑93 (MQ=255)
              tctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGa                                                                                 >  1:7476357/1‑93 (MQ=255)
               ctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGaa                                                                                >  2:7299130/1‑93 (MQ=255)
                 ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAg                                                                              >  2:4442473/1‑93 (MQ=255)
                 ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAg                                                                              <  2:970318/93‑1 (MQ=255)
                  cTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGc                                                                             >  2:3004793/1‑93 (MQ=255)
                    gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCgt                                                                           <  1:3630258/93‑1 (MQ=255)
                    gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGCAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCgtg                                                                          <  1:6212664/93‑1 (MQ=255)
                     cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCgtg                                                                          <  1:1123595/93‑1 (MQ=255)
                     cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCgtg                                                                          <  2:3843457/93‑1 (MQ=255)
                     cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCgtg                                                                          >  2:4129768/1‑93 (MQ=255)
                      aaaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCgtgg                                                                         <  1:6064742/93‑1 (MQ=255)
                      aaaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCgtgg                                                                         <  1:4573148/93‑1 (MQ=255)
                         ccGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTgc                                                                      <  2:4172071/93‑1 (MQ=255)
                              gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGcaca                                                                 <  2:4609128/93‑1 (MQ=255)
                              gggaggAAAGAAAACAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCacac                                                                >  2:7490571/1‑93 (MQ=255)
                               ggaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCacac                                                                <  2:2396346/93‑1 (MQ=255)
                                gaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACAcc                                                               >  1:3804741/1‑93 (MQ=255)
                                 aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGa                                                             <  1:6596335/93‑1 (MQ=255)
                                  ggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGa                                                             <  2:6271832/93‑1 (MQ=255)
                                    aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc                                                           <  1:4450905/93‑1 (MQ=255)
                                    aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGAGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCt                                                          <  2:4976141/93‑1 (MQ=255)
                                     aagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCt                                                          <  1:4535085/93‑1 (MQ=255)
                                      agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTg                                                         >  1:2961944/1‑93 (MQ=255)
                                      agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTg                                                         >  1:7151890/1‑93 (MQ=255)
                                       gaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGc                                                        <  2:1745908/93‑1 (MQ=255)
                                         aaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGt                                                      >  2:1945428/1‑93 (MQ=255)
                                         aaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGt                                                      >  2:3388423/1‑93 (MQ=255)
                                          aagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTa                                                     >  2:1217097/1‑93 (MQ=255)
                                          aagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                                                    <  2:2354911/93‑1 (MQ=255)
                                           agagCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                                                    >  1:3684460/1‑93 (MQ=255)
                                           agagCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                                                    >  2:6556145/1‑93 (MQ=255)
                                            gagCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGc                                                   >  1:6480263/1‑93 (MQ=255)
                                            gagCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg                                                  >  1:1860676/1‑93 (MQ=255)
                                             agCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg                                                  <  1:2255697/93‑1 (MQ=255)
                                                gACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                               >  2:6336217/1‑93 (MQ=255)
                                                gACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                               >  2:4067195/1‑93 (MQ=255)
                                                gACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                               >  2:1543200/1‑93 (MQ=255)
                                                gACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                               >  1:7362907/1‑93 (MQ=255)
                                                gACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                               >  1:5203371/1‑93 (MQ=255)
                                                 aCCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatca                                              >  1:94769/1‑93 (MQ=255)
                                                 aCCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatca                                              >  1:4022655/1‑93 (MQ=255)
                                                   cATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatcatc                                            <  1:5706737/93‑1 (MQ=255)
                                                     ttttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCGCATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATccc                                          >  1:4315444/1‑93 (MQ=255)
                                                     ttttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATccc                                          <  2:5793404/93‑1 (MQ=255)
                                                     ttttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATccc                                          >  2:2316721/1‑93 (MQ=255)
                                                     ttttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATccc                                          <  2:6230717/93‑1 (MQ=255)
                                                      tttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCtt                                        <  1:6432415/93‑1 (MQ=255)
                                                       ttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCtt                                        <  1:1879398/93‑1 (MQ=255)
                                                       ttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCtt                                        <  1:2074856/93‑1 (MQ=255)
                                                         gtggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGc                                      >  2:937359/1‑93 (MQ=255)
                                                         gtggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGc                                      >  1:5535783/1‑93 (MQ=255)
                                                         gtggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGc                                      <  2:5237115/93‑1 (MQ=255)
                                                           ggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTg                                    <  2:561767/93‑1 (MQ=255)
                                                            gcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                   <  2:2619727/93‑1 (MQ=255)
                                                            gcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                   <  2:5542438/93‑1 (MQ=255)
                                                            gcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                   >  1:5055822/1‑93 (MQ=255)
                                                              ggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATa                                 >  1:6254577/1‑93 (MQ=255)
                                                               gtggcgCGCGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaa                                >  2:1671229/1‑93 (MQ=255)
                                                                  gcgcgcGGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAcc                             <  2:6901850/93‑1 (MQ=255)
                                                                   cgcgcgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCa                            <  2:3950709/93‑1 (MQ=255)
                                                                     cgcgGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCa                         <  1:2845008/93‑1 (MQ=255)
                                                                      gcgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCa                         <  1:4866685/93‑1 (MQ=255)
                                                                       cgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                        <  2:2777609/93‑1 (MQ=255)
                                                                       cgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                        <  2:4711518/93‑1 (MQ=255)
                                                                       cgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                        <  2:2462171/93‑1 (MQ=255)
                                                                       cgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                        >  2:2253519/1‑93 (MQ=255)
                                                                       cgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                        <  1:6847499/93‑1 (MQ=255)
                                                                       cgGAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAc                        >  1:67040/1‑93 (MQ=255)
                                                                        ggAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACt                       >  2:5340092/1‑93 (MQ=255)
                                                                         gAAAAATTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTg                      >  2:5911001/1‑93 (MQ=255)
                                                                           aaaaTTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGc                    >  2:2063859/1‑93 (MQ=255)
                                                                           aaaaTTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGc                    >  1:533711/1‑93 (MQ=255)
                                                                            aaaTTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGca                   <  2:3249175/93‑1 (MQ=255)
                                                                             aaTTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcac                  >  1:2379592/1‑93 (MQ=255)
                                                                              aTTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcacc                 >  1:4766819/1‑93 (MQ=255)
                                                                              aTTCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcacc                 >  2:4824006/1‑93 (MQ=255)
                                                                               ttCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcacca                >  1:1499406/1‑93 (MQ=255)
                                                                                tCACACACGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcaccac               <  2:3813202/93‑1 (MQ=255)
                                                                                 cacacaCGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACt              >  1:2618088/1‑93 (MQ=255)
                                                                                 cacacaCGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACt              <  1:7317679/93‑1 (MQ=255)
                                                                                   cacacGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc            >  1:3526082/1‑93 (MQ=255)
                                                                                    acacGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcg           >  2:7096988/1‑93 (MQ=255)
                                                                                    acacGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgc          <  1:1045071/93‑1 (MQ=255)
                                                                                      acGGTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgcg         >  1:5907103/1‑93 (MQ=255)
                                                                                        ggTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCggg       <  1:1742838/93‑1 (MQ=255)
                                                                                        ggTAGCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCggg       <  1:589198/93‑1 (MQ=255)
                                                                                            g‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGTGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtgg  <  2:2660064/93‑1 (MQ=255)
                                                                                             |                                                                                            
AAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGG  >  NC_000913/3506221‑3506405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTG  >  NC_000913/3506226‑3506411
                                                                                              |                                                                                             
CATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCC                                                                                              >  SRR4302542.20334/1‑94 (MQ=60)
CATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAA‑‑CCCCCC                                                                                              >  SRR4302542.7243136/1‑94 (MQ=60)
 ATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTCGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCAC                                                                                             <  SRR4302542.6277925/94‑1 (MQ=60)
  TAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCcac                                                                                            >  SRR4302542.5656767/1‑91 (MQ=60)
   AGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACA                                                                                            >  SRR4302542.3155313/1‑94 (MQ=60)
    GGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACAT                                                                                           >  SRR4302542.4388503/1‑94 (MQ=60)
    GGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACAT                                                                                           >  SRR4302542.566759/1‑94 (MQ=60)
    GGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACAT                                                                                           <  SRR4302542.3008823/94‑1 (MQ=60)
     GGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATC                                                                                          >  SRR4302542.4972080/1‑94 (MQ=60)
      GTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCA                                                                                         <  SRR4302542.1810/94‑1 (MQ=60)
      GTGTCTACTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCA                                                                                         <  SRR4302542.1455368/94‑1 (MQ=60)
       TGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAG                                                                                        <  SRR4302542.4792732/94‑1 (MQ=60)
       TGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAG                                                                                        <  SRR4302542.3858758/94‑1 (MQ=60)
        GTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCCGA                                                                                       >  SRR4302542.7549624/1‑94 (MQ=60)
        GTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGA                                                                                       >  SRR4302542.7549625/1‑94 (MQ=60)
          CTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAA                                                                                     <  SRR4302542.5549919/94‑1 (MQ=60)
          CTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAA                                                                                     >  SRR4302542.6542720/1‑94 (MQ=60)
           TCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAG                                                                                    <  SRR4302542.4182096/94‑1 (MQ=60)
            CCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGC                                                                                   <  SRR4302542.4607315/94‑1 (MQ=60)
            CCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGC                                                                                   >  SRR4302542.2752381/1‑94 (MQ=60)
             CTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCG                                                                                  <  SRR4302542.3172722/94‑1 (MQ=60)
              TGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGT                                                                                 <  SRR4302542.4186377/94‑1 (MQ=60)
               GCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTG                                                                                <  SRR4302542.1612665/94‑1 (MQ=60)
               GCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTG                                                                                <  SRR4302542.3664080/94‑1 (MQ=60)
               GCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGCAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGG                                                                               <  SRR4302542.6273878/94‑1 (MQ=60)
                CAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGG                                                                               <  SRR4302542.2461193/94‑1 (MQ=60)
                CAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGG                                                                               <  SRR4302542.1133191/94‑1 (MQ=60)
                CAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGG                                                                               >  SRR4302542.5368384/1‑94 (MQ=60)
                 AAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGT                                                                              <  SRR4302542.479292/94‑1 (MQ=60)
                 AAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGT                                                                              <  SRR4302542.6123486/94‑1 (MQ=60)
                 AAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGT                                                                              >  SRR4302542.1486831/1‑94 (MQ=60)
                 AAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGT                                                                              <  SRR4302542.4617899/94‑1 (MQ=60)
                  AACCGGAGGGAGAAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTG                                                                             <  SRR4302542.3359656/94‑1 (MQ=60)
                   ACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGC                                                                            >  SRR4302542.5221427/1‑94 (MQ=60)
                   ACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGC                                                                            >  SRR4302542.4838660/1‑94 (MQ=60)
                    CCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCG                                                                           >  SRR4302542.2843156/1‑94 (MQ=60)
                     CGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGC                                                                          >  SRR4302542.5370317/1‑94 (MQ=60)
                     CGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGC                                                                          >  SRR4302542.735882/1‑94 (MQ=60)
                       GAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCGC                                                                        <  SRR4302542.6017278/94‑1 (MQ=60)
                       GAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCAC                                                                        <  SRR4302542.1469075/94‑1 (MQ=60)
                       GAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCAC                                                                        <  SRR4302542.67112/94‑1 (MQ=60)
                       GAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACA                                                                       <  SRR4302542.2259133/94‑1 (MQ=60)
                         GGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACAC                                                                      >  SRR4302542.1393065/1‑94 (MQ=60)
                         GGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACAC                                                                      <  SRR4302542.6320314/94‑1 (MQ=60)
                          GGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACC                                                                     >  SRR4302542.3842393/1‑94 (MQ=60)
                           GAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCG                                                                    <  SRR4302542.5282656/94‑1 (MQ=60)
                            AGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGA                                                                   >  SRR4302542.1887721/1‑94 (MQ=60)
                            AGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGA                                                                   >  SRR4302542.6019023/1‑94 (MQ=60)
                            AGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAG                                                                  <  SRR4302542.6661329/94‑1 (MQ=60)
                               AAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCT                                                                >  SRR4302542.5128794/1‑94 (MQ=60)
                               AAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCT                                                                <  SRR4302542.790361/94‑1 (MQ=60)
                               AAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCT                                                                >  SRR4302542.4898078/1‑94 (MQ=60)
                               AAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCT                                                                <  SRR4302542.4494561/94‑1 (MQ=60)
                                AAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTG                                                               >  SRR4302542.2990327/1‑94 (MQ=60)
                                AAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTG                                                               <  SRR4302542.4579495/94‑1 (MQ=60)
                                AAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTG                                                               >  SRR4302542.7222058/1‑94 (MQ=60)
                                AAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTG                                                               <  SRR4302542.3491602/94‑1 (MQ=60)
                                AAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTG                                                               <  SRR4302542.7444187/94‑1 (MQ=60)
                                  GAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCG                                                             <  SRR4302542.3702775/94‑1 (MQ=60)
                                    AAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTA                                                           >  SRR4302542.375590/1‑94 (MQ=60)
                                    AAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTA                                                           <  SRR4302542.1573009/94‑1 (MQ=60)
                                    AAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTA                                                           >  SRR4302542.1821402/1‑94 (MQ=60)
                                     AAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAG                                                          >  SRR4302542.3720700/1‑94 (MQ=60)
                                     AAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAG                                                          <  SRR4302542.3428795/94‑1 (MQ=60)
                                      AGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGC                                                         >  SRR4302542.6544106/1‑94 (MQ=60)
                                      AGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGC                                                         <  SRR4302542.571897/94‑1 (MQ=60)
                                      AGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGC                                                         >  SRR4302542.2280823/1‑94 (MQ=60)
                                      AGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCG                                                        <  SRR4302542.5626077/94‑1 (MQ=60)
                                      AGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCG                                                        >  SRR4302542.1876780/1‑94 (MQ=60)
                                        AGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGA                                                       <  SRR4302542.2275090/94‑1 (MQ=60)
                                          CGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC                                                     >  SRR4302542.5253938/1‑94 (MQ=60)
                                          CGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC                                                     >  SRR4302542.7435028/1‑94 (MQ=60)
                                           GCCCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCA                                                    <  SRR4302542.6295827/94‑1 (MQ=60)
                                           GACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATC                                                     <  SRR4302542.6686583/94‑1 (MQ=60)
                                           GACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCA                                                    >  SRR4302542.4062346/1‑94 (MQ=60)
                                           GACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCA                                                    >  SRR4302542.95576/1‑94 (MQ=60)
                                            TCCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCAT                                                   <  SRR4302542.4418677/94‑1 (MQ=60)
                                             CGATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATC                                                  >  SRR4302542.4921567/1‑94 (MQ=60)
                                              CATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCC                                                 <  SRR4302542.5762131/94‑1 (MQ=60)
                                              CATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCC                                                 >  SRR4302542.7167711/1‑94 (MQ=60)
                                               ATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCGCATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCC                                                >  SRR4302542.4357841/1‑94 (MQ=60)
                                               ATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCC                                                >  SRR4302542.6904025/1‑94 (MQ=60)
                                               ATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCC                                                >  SRR4302542.1123390/1‑94 (MQ=60)
                                                TTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCT                                               <  SRR4302542.4536621/94‑1 (MQ=60)
                                                TTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTT                                              >  SRR4302542.2435804/1‑94 (MQ=60)
                                                 TTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTG                                             <  SRR4302542.6495790/94‑1 (MQ=60)
                                                  TTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTG                                             <  SRR4302542.2092787/94‑1 (MQ=60)
                                                  TTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTG                                             <  SRR4302542.1895669/94‑1 (MQ=60)
                                                  TTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTG                                             <  SRR4302542.5559275/94‑1 (MQ=60)
                                                   TGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACGCCGGGCTGCGTAGCGATCATCCCTTGC                                            <  SRR4302542.7406756/94‑1 (MQ=60)
                                                   TGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGC                                            >  SRR4302542.5589596/1‑94 (MQ=60)
                                                      GGCGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGA                                         >  SRR4302542.5104920/1‑94 (MQ=60)
                                                        CGGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATA                                       >  SRR4302542.6316205/1‑94 (MQ=60)
                                                         GGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAA                                      >  SRR4302542.3622747/1‑94 (MQ=60)
                                                         GGTGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAA                                      <  SRR4302542.4520606/94‑1 (MQ=60)
                                                           TGGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAC                                    <  SRR4302542.571013/94‑1 (MQ=60)
                                                            GGCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACC                                   <  SRR4302542.2131650/94‑1 (MQ=60)
                                                             GCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCA                                  >  SRR4302542.6810608/1‑94 (MQ=60)
                                                             GCGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCCGATAAACCA                                  <  SRR4302542.2001036/94‑1 (MQ=60)
                                                             GCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAG                                 <  SRR4302542.1295054/94‑1 (MQ=60)
                                                              CGCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAG                                 >  SRR4302542.3928393/1‑94 (MQ=60)
                                                              CGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGC                                >  SRR4302542.3658713/1‑94 (MQ=60)
                                                               GCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGC                                <  SRR4302542.6223367/94‑1 (MQ=60)
                                                               GCGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGC                                >  SRR4302542.6417103/1‑94 (MQ=60)
                                                               GCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCA                               <  SRR4302542.2879729/94‑1 (MQ=60)
                                                                CGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCA                               >  SRR4302542.7587995/1‑94 (MQ=60)
                                                                CGCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCA                               <  SRR4302542.6574325/94‑1 (MQ=60)
                                                                CGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAC                              <  SRR4302542.2872345/94‑1 (MQ=60)
                                                                 GCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAC                              <  SRR4302542.4914065/94‑1 (MQ=60)
                                                                 GCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAC                              <  SRR4302542.7328999/94‑1 (MQ=60)
                                                                 GCGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCAC                              >  SRR4302542.67605/1‑94 (MQ=60)
                                                                  CGGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACT                             <  SRR4302542.6914754/94‑1 (MQ=60)
                                                                   GGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTG                            >  SRR4302542.6571598/1‑94 (MQ=60)
                                                                   GGAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTG                            >  SRR4302542.4467781/1‑94 (MQ=60)
                                                                    GAAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGG                           >  SRR4302542.595167/1‑94 (MQ=60)
                                                                     AAAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGC                          >  SRR4302542.538322/1‑94 (MQ=60)
                                                                      AAAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCA                         >  SRR4302542.7392611/1‑94 (MQ=60)
                                                                       AAATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCAC                        >  SRR4302542.2401170/1‑94 (MQ=60)
                                                                        AATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACC                       >  SRR4302542.4813283/1‑94 (MQ=60)
                                                                         ATTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCA                      >  SRR4302542.1512373/1‑94 (MQ=60)
                                                                          TTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCAC                     <  SRR4302542.5774032/94‑1 (MQ=60)
                                                                          TTCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCAC                     >  SRR4302542.7127018/1‑94 (MQ=60)
                                                                           TCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACT                    >  SRR4302542.2643389/1‑94 (MQ=60)
                                                                           TCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACT                    <  SRR4302542.5638025/94‑1 (MQ=60)
                                                                           TCACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACT                    <  SRR4302542.3822158/94‑1 (MQ=60)
                                                                            CACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTT                   <  SRR4302542.7389354/94‑1 (MQ=60)
                                                                             ACACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTC                  >  SRR4302542.3559036/1‑94 (MQ=60)
                                                                              CACACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCG                 >  SRR4302542.6195256/1‑94 (MQ=60)
                                                                               ACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCG               <  SRR4302542.1054037/94‑1 (MQ=60)
                                                                                CACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCG               >  SRR4302542.5964346/1‑94 (MQ=60)
                                                                                CACGGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCG               >  SRR4302542.3090200/1‑94 (MQ=60)
                                                                                   GGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGT            <  SRR4302542.594277/94‑1 (MQ=60)
                                                                                   GGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGT            <  SRR4302542.1757966/94‑1 (MQ=60)
                                                                                   GGTAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGT            >  SRR4302542.4954752/1‑94 (MQ=60)
                                                                                     TAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAT          >  SRR4302542.4777367/1‑94 (MQ=60)
                                                                                     TAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAT          <  SRR4302542.1103649/94‑1 (MQ=60)
                                                                                     TAGC‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAT          <  SRR4302542.5675372/94‑1 (MQ=60)
                                                                                       agcCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATG         >  SRR4302542.2938286/4‑94 (MQ=60)
                                                                                       agcCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATG         >  SRR4302542.698387/4‑94 (MQ=60)
                                                                                       agcCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATG         >  SRR4302542.7028955/4‑94 (MQ=60)
                                                                                       C‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGT       <  SRR4302542.6935769/94‑1 (MQ=60)
                                                                                       C‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGT       >  SRR4302542.3090464/1‑94 (MQ=60)
                                                                                       C‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGT       >  SRR4302542.4578508/1‑94 (MQ=60)
                                                                                          CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTG      <  SRR4302542.354869/94‑1 (MQ=60)
                                                                                          CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTG      <  SRR4302542.3137749/94‑1 (MQ=60)
                                                                                          CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTG      <  SRR4302542.3950856/94‑1 (MQ=60)
                                                                                           CCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGG     >  SRR4302542.6843592/1‑94 (MQ=60)
                                                                                           CCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGG     >  SRR4302542.2375219/1‑94 (MQ=60)
                                                                                           CCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGG     <  SRR4302542.7539848/94‑1 (MQ=60)
                                                                                           CCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGG     <  SRR4302542.1598490/94‑1 (MQ=60)
                                                                                           CCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGG     >  SRR4302542.1466237/1‑94 (MQ=60)
                                                                                           CCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGG     >  SRR4302542.6906922/1‑94 (MQ=60)
                                                                                            CCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGC    >  SRR4302542.4704772/1‑94 (MQ=60)
                                                                                            CCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGC    >  SRR4302542.2077070/1‑94 (MQ=60)
                                                                                            CCCACATCAGAAAGCGTGGTGCGCAAACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGC    >  SRR4302542.3730005/1‑94 (MQ=60)
                                                                                              CACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTG  >  SRR4302542.6592318/1‑94 (MQ=60)
                                                                                              CACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTG  <  SRR4302542.243672/94‑1 (MQ=60)
                                                                                              CACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTG  >  SRR4302542.2010189/1‑94 (MQ=60)
                                                                                              |                                                                                             
CATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAG‑‑CCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTG  >  NC_000913/3506226‑3506411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑