Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,579,035 T→C R89R (CGT→CGC)  yhhY → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,579,0350TC89.3% 785.9 / 75.7 299R89R (CGT→CGC) yhhYhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/19);  new base C (117/150);  total (130/169)
Fisher's exact test for biased strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 300 total aligned reads displayed.

TGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGT  >  NC_000913/3578945‑3579127
                                                                                          |                                                                                            
tGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTg                                                                                            >  1:1239876/1‑93 (MQ=255)
tGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTg                                                                                            >  1:3597123/1‑93 (MQ=255)
tGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTg                                                                                            <  2:2488057/93‑1 (MQ=255)
   agaCGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGgaa                                                                                         <  2:330772/93‑1 (MQ=255)
   agaCGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGgaa                                                                                         <  2:2140711/93‑1 (MQ=255)
    gaCGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGgaag                                                                                        >  2:661392/1‑93 (MQ=255)
     aCGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGgaaga                                                                                       >  2:1168125/1‑93 (MQ=255)
      cgtcgtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGgaagaa                                                                                      <  1:1207777/93‑1 (MQ=255)
      cgtcgtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGgaagaa                                                                                      <  2:1025067/93‑1 (MQ=255)
       gtcgtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAAc                                                                                     <  2:6108111/93‑1 (MQ=255)
       gtcgtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAAc                                                                                     >  2:6682128/1‑93 (MQ=255)
        tcgtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAAcc                                                                                    <  2:2596076/93‑1 (MQ=255)
         cgtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACcg                                                                                   <  1:4964262/93‑1 (MQ=255)
         cgtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACcg                                                                                   <  1:2019379/93‑1 (MQ=255)
          gtGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACcgc                                                                                  <  1:1820619/93‑1 (MQ=255)
           tGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACcgcg                                                                                 >  1:2635447/1‑93 (MQ=255)
            gggCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCgcgg                                                                                >  1:5552262/1‑93 (MQ=255)
            gggCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCgcgg                                                                                <  2:5053107/93‑1 (MQ=255)
              gCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCgcggcg                                                                              <  1:5329055/93‑1 (MQ=255)
               ccATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGt                                                                             <  1:3036880/93‑1 (MQ=255)
                cATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTc                                                                            <  1:2258110/93‑1 (MQ=255)
                  tctcACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGc                                                                          <  2:3258115/93‑1 (MQ=255)
                  tctcACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGc                                                                          <  2:7526297/93‑1 (MQ=255)
                   ctcACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGcc                                                                         <  2:5503964/93‑1 (MQ=255)
                   ctcACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGcc                                                                         <  1:810988/93‑1 (MQ=255)
                      aCCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCTATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAgc                                                                      <  2:4240354/93‑1 (MQ=255)
                      aCCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAgc                                                                      >  1:1974303/1‑93 (MQ=255)
                      aCCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAgc                                                                      >  2:4451381/1‑93 (MQ=255)
                        cATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAgcgc                                                                    >  2:2224135/1‑93 (MQ=255)
                         aTTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGcc                                                                   >  1:6798657/1‑93 (MQ=255)
                           tGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCt                                                                 >  2:2910471/1‑93 (MQ=255)
                           tGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCt                                                                 <  1:5951305/93‑1 (MQ=255)
                            gACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTg                                                                >  2:3005610/1‑93 (MQ=255)
                            gACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTg                                                                >  2:2686300/1‑93 (MQ=255)
                             aCGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGa                                                               <  1:2053982/93‑1 (MQ=255)
                             aCGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGa                                                               <  1:6442993/93‑1 (MQ=255)
                              cGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAt                                                              <  1:46972/93‑1 (MQ=255)
                              cGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAt                                                              <  1:1038961/93‑1 (MQ=255)
                              cGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAt                                                              <  2:5778972/93‑1 (MQ=255)
                              cGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAt                                                              <  1:6145619/93‑1 (MQ=255)
                               gTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATg                                                             >  2:7168450/1‑93 (MQ=255)
                                tGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGc                                                            >  2:5218234/1‑93 (MQ=255)
                                tGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTACCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGc                                                            <  1:7371928/93‑1 (MQ=255)
                                  cAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCga                                                          <  1:2764364/93‑1 (MQ=255)
                                   aaCAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgag                                                         <  1:6926395/93‑1 (MQ=255)
                                    aCAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgaga                                                        <  2:3503787/93‑1 (MQ=255)
                                    aCAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgaga                                                        >  2:38006/1‑93 (MQ=255)
                                     cAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgagag                                                       <  1:482550/93‑1 (MQ=255)
                                       gcgcCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgat                                                     <  2:6408194/93‑1 (MQ=255)
                                         gcCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgatga                                                   <  2:368319/93‑1 (MQ=255)
                                         gcCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgatga                                                   <  2:1454603/93‑1 (MQ=255)
                                         gcCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgatga                                                   <  2:6007497/93‑1 (MQ=255)
                                         gcCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgatga                                                   <  1:3714804/93‑1 (MQ=255)
                                          cccACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgatgat                                                  <  2:7292807/93‑1 (MQ=255)
                                          cccACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgatgat                                                  >  2:5941211/1‑93 (MQ=255)
                                           ccACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGAtt                                                 <  2:128981/93‑1 (MQ=255)
                                               gccgcAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGaaa                                             <  2:3197298/93‑1 (MQ=255)
                                                 cgcAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtg                                           <  2:1771042/93‑1 (MQ=255)
                                                  gcAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtgt                                          >  2:4090124/1‑93 (MQ=255)
                                                  gcAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtgt                                          <  2:6179516/93‑1 (MQ=255)
                                                  gcAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtgt                                          >  1:915453/1‑93 (MQ=255)
                                                  gcAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtgt                                          >  1:5794505/1‑93 (MQ=255)
                                                  gcAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtgt                                          >  2:2104732/1‑93 (MQ=255)
                                                   cAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtgtg                                         <  1:2134049/93‑1 (MQ=255)
                                                    aGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGc                                        <  1:3240271/93‑1 (MQ=255)
                                                      tCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGa                                      <  1:4020709/93‑1 (MQ=255)
                                                       cATTTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAc                                     >  1:6969063/1‑93 (MQ=255)
                                                       cATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAc                                     >  1:6239473/1‑93 (MQ=255)
                                                       cATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAc                                     <  1:4418682/93‑1 (MQ=255)
                                                       cATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAc                                     <  1:1698959/93‑1 (MQ=255)
                                                         tGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACaa                                   >  1:1431821/1‑93 (MQ=255)
                                                          gTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAAc                                  <  1:6092801/93‑1 (MQ=255)
                                                           ttGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACt                                 <  2:7387098/93‑1 (MQ=255)
                                                           ttGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACt                                 <  1:4203477/93‑1 (MQ=255)
                                                            tGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTg                                <  2:1340100/93‑1 (MQ=255)
                                                            tGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTg                                <  2:200715/93‑1 (MQ=255)
                                                            tGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTg                                >  1:5187608/1‑93 (MQ=255)
                                                             gCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTgg                               <  1:3852527/93‑1 (MQ=255)
                                                             gCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTgg                               <  2:241374/93‑1 (MQ=255)
                                                              ccGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGt                              <  1:660398/93‑1 (MQ=255)
                                                               cGATTTTGGTATCTGTGTCGGCTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGtt                             >  2:6879512/1‑93 (MQ=255)
                                                               cGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGtt                             >  2:1609182/1‑93 (MQ=255)
                                                               cGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGtt                             <  2:5155941/93‑1 (MQ=255)
                                                               cGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGtt                             <  2:2893941/93‑1 (MQ=255)
                                                                  ttttGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCg                          >  2:411254/1‑93 (MQ=255)
                                                                  ttttGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCg                          <  1:5618909/93‑1 (MQ=255)
                                                                   tttGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCgg                         <  2:6434388/93‑1 (MQ=255)
                                                                     tGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGt                       <  1:3434810/93‑1 (MQ=255)
                                                                     tGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGt                       <  1:2872557/93‑1 (MQ=255)
                                                                      ggTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTa                      <  2:1412612/93‑1 (MQ=255)
                                                                      ggTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTa                      <  2:56583/93‑1 (MQ=255)
                                                                        tATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGa                    <  1:7435527/93‑1 (MQ=255)
                                                                          tCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATc                  >  2:649123/1‑93 (MQ=255)
                                                                             gtgtCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCa               >  2:3915090/1‑93 (MQ=255)
                                                                                 cGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGa           >  2:166128/1‑93 (MQ=255)
                                                                                   aCTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAAc         <  1:4178888/93‑1 (MQ=255)
                                                                                   aCTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAAc         <  1:5734791/93‑1 (MQ=255)
                                                                                      ctcGCTGGAAGAACCGCGGCGTCGTCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTaa      <  2:4911064/93‑1 (MQ=255)
                                                                                      ctcGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTaa      <  2:4382439/93‑1 (MQ=255)
                                                                                       tcGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAAc     >  2:4615211/1‑93 (MQ=255)
                                                                                          |                                                                                            
TGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGT  >  NC_000913/3578945‑3579127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTG  >  NC_000913/3578942‑3579128
                                                                                             |                                                                                             
TGATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGC                                                                                               >  SRR4302542.5242554/1‑94 (MQ=60)
TGATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGC                                                                                               >  SRR4302542.6720639/1‑94 (MQ=60)
 GATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCT                                                                                              <  SRR4302542.3918199/94‑1 (MQ=60)
 GATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCT                                                                                              <  SRR4302542.840136/94‑1 (MQ=60)
  ATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTG                                                                                             >  SRR4302542.1250530/1‑94 (MQ=60)
  ATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTG                                                                                             >  SRR4302542.3630662/1‑94 (MQ=60)
    GGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGA                                                                                           >  SRR4302542.1317309/1‑94 (MQ=60)
      AGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAG                                                                                         <  SRR4302542.5033809/94‑1 (MQ=60)
       GACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGA                                                                                        >  SRR4302542.5246972/1‑94 (MQ=60)
         CGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAAC                                                                                      <  SRR4302542.1218158/94‑1 (MQ=60)
         CGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAAC                                                                                      <  SRR4302542.1218159/94‑1 (MQ=60)
          GTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACC                                                                                     >  SRR4302542.536898/1‑94 (MQ=60)
           TCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCG                                                                                    >  SRR4302542.1910799/1‑94 (MQ=60)
           TCGTGGGCCATCTCACCATTGACGTGC‑ACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGC                                                                                   <  SRR4302542.2036847/94‑1 (MQ=60)
            CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGC                                                                                   <  SRR4302542.2036846/94‑1 (MQ=60)
            CGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGC                                                                                   <  SRR4302542.5012473/94‑1 (MQ=60)
             GTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCG                                                                                  >  SRR4302542.2660898/1‑94 (MQ=60)
             GTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCG                                                                                  >  SRR4302542.1787768/1‑94 (MQ=60)
             GTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCG                                                                                  <  SRR4302542.1836385/94‑1 (MQ=60)
              TGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGG                                                                                 >  SRR4302542.5606211/1‑94 (MQ=60)
              TGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGG                                                                                 <  SRR4302542.4222528/94‑1 (MQ=60)
              TGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGG                                                                                 >  SRR4302542.997683/1‑94 (MQ=60)
              TGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGG                                                                                 >  SRR4302542.7477958/1‑94 (MQ=60)
               GGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGC                                                                                <  SRR4302542.1093483/94‑1 (MQ=60)
               GGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGC                                                                                <  SRR4302542.4157699/94‑1 (MQ=60)
                GGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCG                                                                               <  SRR4302542.6954982/94‑1 (MQ=60)
                GGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCG                                                                               <  SRR4302542.782329/94‑1 (MQ=60)
                 GCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGT                                                                              <  SRR4302542.5380830/94‑1 (MQ=60)
                  CCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTC                                                                             <  SRR4302542.3065846/94‑1 (MQ=60)
                   CATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCG                                                                            <  SRR4302542.6984536/94‑1 (MQ=60)
                   CATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCG                                                                            <  SRR4302542.2277522/94‑1 (MQ=60)
                    ATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGC                                                                           >  SRR4302542.2763774/1‑94 (MQ=60)
                     TCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCC                                                                          >  SRR4302542.7357402/1‑94 (MQ=60)
                     TCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCC                                                                          >  SRR4302542.1965970/1‑94 (MQ=60)
                      CTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCA                                                                         <  SRR4302542.818007/94‑1 (MQ=60)
                       TCACCATTGACGTGCAACAGCGCCCACGCCGCCGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAC                                                                        >  SRR4302542.3763242/1‑94 (MQ=60)
                       TCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAG                                                                        <  SRR4302542.4879084/94‑1 (MQ=60)
                        CACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGC                                                                       <  SRR4302542.2270535/94‑1 (MQ=60)
                        CACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGC                                                                       >  SRR4302542.1991358/1‑94 (MQ=60)
                          CCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGC                                                                     >  SRR4302542.744094/1‑94 (MQ=60)
                           CATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCC                                                                    >  SRR4302542.6283597/1‑94 (MQ=60)
                           CATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCC                                                                    >  SRR4302542.1232266/1‑94 (MQ=60)
                           CATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCC                                                                    >  SRR4302542.6865510/1‑94 (MQ=60)
                            ATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCC                                                                   <  SRR4302542.4032845/94‑1 (MQ=60)
                              TGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTG                                                                 >  SRR4302542.27544/1‑94 (MQ=60)
                              TGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTG                                                                 <  SRR4302542.6008943/94‑1 (MQ=60)
                                ACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTTGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAT                                                               <  SRR4302542.6199841/94‑1 (MQ=60)
                                ACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAT                                                               <  SRR4302542.2071736/94‑1 (MQ=60)
                                ACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAT                                                               <  SRR4302542.183536/94‑1 (MQ=60)
                                ACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAT                                                               <  SRR4302542.6506443/94‑1 (MQ=60)
                                 CGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATG                                                              <  SRR4302542.6205076/94‑1 (MQ=60)
                                 CGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATG                                                              <  SRR4302542.1047885/94‑1 (MQ=60)
                                 CGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATG                                                              <  SRR4302542.47364/94‑1 (MQ=60)
                                  GTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGC                                                             <  SRR4302542.5560919/94‑1 (MQ=60)
                                   TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTACCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCG                                                            <  SRR4302542.7444119/94‑1 (MQ=60)
                                    GCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGA                                                           <  SRR4302542.6331924/94‑1 (MQ=60)
                                     CAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAG                                                          <  SRR4302542.2791003/94‑1 (MQ=60)
                                      AACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGA                                                         <  SRR4302542.6994399/94‑1 (MQ=60)
                                       ACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAG                                                        <  SRR4302542.2801056/94‑1 (MQ=60)
                                       ACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAACGCCCTGATGCGAGAG                                                        <  SRR4302542.3556013/94‑1 (MQ=60)
                                        CAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGA                                                       <  SRR4302542.486732/94‑1 (MQ=60)
                                        CAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGA                                                       <  SRR4302542.337485/94‑1 (MQ=60)
                                         AGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGAT                                                      <  SRR4302542.167495/94‑1 (MQ=60)
                                          GCGCCCACGCCGCAGTCATGTTGCCGATTTTTGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAAATG                                                     <  SRR4302542.1054298/94‑1 (MQ=60)
                                           CGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGA                                                    >  SRR4302542.810227/1‑94 (MQ=60)
                                           CGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGA                                                    >  SRR4302542.449098/1‑94 (MQ=60)
                                            GCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGAT                                                   <  SRR4302542.3751556/94‑1 (MQ=60)
                                               CACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGA                                                >  SRR4302542.2108836/1‑94 (MQ=60)
                                                 CGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAA                                              >  SRR4302542.4774494/1‑94 (MQ=60)
                                                  GCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAT                                             <  SRR4302542.2030309/94‑1 (MQ=60)
                                                    CGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGT                                           <  SRR4302542.4710141/94‑1 (MQ=60)
                                                    CGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGT                                           >  SRR4302542.3725122/1‑94 (MQ=60)
                                                    CGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGT                                           >  SRR4302542.5850747/1‑94 (MQ=60)
                                                    CGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGT                                           >  SRR4302542.909572/1‑94 (MQ=60)
                                                    CGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGT                                           >  SRR4302542.923349/1‑94 (MQ=60)
                                                     GCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTG                                          <  SRR4302542.4369538/94‑1 (MQ=60)
                                                     GCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTG                                          >  SRR4302542.5816565/1‑94 (MQ=60)
                                                      CAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGC                                         >  SRR4302542.6037520/1‑94 (MQ=60)
                                                      CAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGC                                         <  SRR4302542.2152468/94‑1 (MQ=60)
                                                      CAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGC                                         >  SRR4302542.6404474/1‑94 (MQ=60)
                                                       AGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCG                                        <  SRR4302542.3833313/94‑1 (MQ=60)
                                                       AGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCG                                        <  SRR4302542.5677725/94‑1 (MQ=60)
                                                       AGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCG                                        <  SRR4302542.3270903/94‑1 (MQ=60)
                                                       AGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCG                                        >  SRR4302542.207146/1‑94 (MQ=60)
                                                       AGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCG                                        >  SRR4302542.4895816/1‑94 (MQ=60)
                                                        GTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGA                                       >  SRR4302542.2986079/1‑94 (MQ=60)
                                                         TCATTTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAC                                      >  SRR4302542.7037525/1‑94 (MQ=60)
                                                         TCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAC                                      <  SRR4302542.4060385/94‑1 (MQ=60)
                                                         TCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAC                                      >  SRR4302542.6300904/1‑94 (MQ=60)
                                                         TCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGAC                                      <  SRR4302542.5808463/94‑1 (MQ=60)
                                                          CATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACA                                     >  SRR4302542.2642651/1‑94 (MQ=60)
                                                          CATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACA                                     <  SRR4302542.1713680/94‑1 (MQ=60)
                                                          CATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACA                                     >  SRR4302542.6123801/1‑94 (MQ=60)
                                                          CATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACA                                     <  SRR4302542.4462081/94‑1 (MQ=60)
                                                          CATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACA                                     >  SRR4302542.6615160/1‑94 (MQ=60)
                                                           ATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA                                    >  SRR4302542.1444198/1‑94 (MQ=60)
                                                           ATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA                                    >  SRR4302542.6682347/1‑94 (MQ=60)
                                                           ATGTTGCCGATTTTGGTATCTGTGTCGACTCCCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA                                    >  SRR4302542.1700401/1‑94 (MQ=60)
                                                            TGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAAC                                   <  SRR4302542.5657311/94‑1 (MQ=60)
                                                            TGTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAAC                                   <  SRR4302542.3600729/94‑1 (MQ=60)
                                                             GTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACT                                  <  SRR4302542.6151778/94‑1 (MQ=60)
                                                             GTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACT                                  <  SRR4302542.4821704/94‑1 (MQ=60)
                                                             GTTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACT                                  <  SRR4302542.433194/94‑1 (MQ=60)
                                                              TTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTG                                 >  SRR4302542.5238003/1‑94 (MQ=60)
                                                              TTGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTG                                 <  SRR4302542.4244920/94‑1 (MQ=60)
                                                               TGCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGG                                >  SRR4302542.4052940/1‑94 (MQ=60)
                                                                GCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGT                               <  SRR4302542.973582/94‑1 (MQ=60)
                                                                GCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGT                               <  SRR4302542.3890584/94‑1 (MQ=60)
                                                                GCCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGT                               <  SRR4302542.5164097/94‑1 (MQ=60)
                                                                 CCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTT                              <  SRR4302542.6656304/94‑1 (MQ=60)
                                                                 CCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTT                              <  SRR4302542.666089/94‑1 (MQ=60)
                                                                 CCGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTT                              <  SRR4302542.7268614/94‑1 (MQ=60)
                                                                  CGATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTG                             >  SRR4302542.7113102/1‑94 (MQ=60)
                                                                    ATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCG                           <  SRR4302542.6007292/94‑1 (MQ=60)
                                                                    ATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCG                           <  SRR4302542.313258/94‑1 (MQ=60)
                                                                    ATTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCG                           <  SRR4302542.5647318/94‑1 (MQ=60)
                                                                     TTTTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGG                          <  SRR4302542.5673492/94‑1 (MQ=60)
                                                                      TTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGG                         <  SRR4302542.4174526/94‑1 (MQ=60)
                                                                       TTGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGT                        <  SRR4302542.5422794/94‑1 (MQ=60)
                                                                        TGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTA                       <  SRR4302542.3466967/94‑1 (MQ=60)
                                                                        TGGTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTA                       <  SRR4302542.2900145/94‑1 (MQ=60)
                                                                          GTATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGA                     >  SRR4302542.5935734/1‑94 (MQ=60)
                                                                           TATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGAT                    <  SRR4302542.7508303/94‑1 (MQ=60)
                                                                            ATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATC                   <  SRR4302542.6218259/94‑1 (MQ=60)
                                                                            ATCTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATC                   >  SRR4302542.885277/1‑94 (MQ=60)
                                                                              CTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGC                 >  SRR4302542.3061886/1‑94 (MQ=60)
                                                                              CTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGC                 >  SRR4302542.2031122/1‑94 (MQ=60)
                                                                              CTGTGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGC                 >  SRR4302542.7018084/1‑94 (MQ=60)
                                                                               TGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCA                <  SRR4302542.6174047/94‑1 (MQ=60)
                                                                                GTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCAT               <  SRR4302542.2466520/94‑1 (MQ=60)
                                                                                 TGTCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATT              <  SRR4302542.3696390/94‑1 (MQ=60)
                                                                                   TCGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGA            <  SRR4302542.125796/94‑1 (MQ=60)
                                                                                    CGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAA           >  SRR4302542.5703769/1‑94 (MQ=60)
                                                                                    CGACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAA           >  SRR4302542.333103/1‑94 (MQ=60)
                                                                                     GACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAAC          <  SRR4302542.4222586/94‑1 (MQ=60)
                                                                                      ACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACT         <  SRR4302542.2793155/94‑1 (MQ=60)
                                                                                      ACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACT         <  SRR4302542.5790456/94‑1 (MQ=60)
                                                                                      ACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACT         <  SRR4302542.4220098/94‑1 (MQ=60)
                                                                                      ACTCTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACT         >  SRR4302542.2183839/1‑94 (MQ=60)
                                                                                         CTCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAAC      >  SRR4302542.3086314/1‑94 (MQ=60)
                                                                                          TCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACC     <  SRR4302542.2045783/94‑1 (MQ=60)
                                                                                          TCGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACC     <  SRR4302542.6807616/94‑1 (MQ=60)
                                                                                           CGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCG    <  SRR4302542.1835936/94‑1 (MQ=60)
                                                                                           CGCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCG    <  SRR4302542.598567/94‑1 (MQ=60)
                                                                                            GCTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGT   >  SRR4302542.7403786/1‑94 (MQ=60)
                                                                                             TTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTG  >  SRR4302542.2460610/1‑94 (MQ=60)
                                                                                             CTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTG  <  SRR4302542.32724/94‑1 (MQ=60)
                                                                                             CTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTG  >  SRR4302542.5891063/1‑94 (MQ=60)
                                                                                             CTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTG  <  SRR4302542.2069269/94‑1 (MQ=60)
                                                                                             CTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTG  >  SRR4302542.106530/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTG  >  NC_000913/3578942‑3579128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑