Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,580,138 A→G L215L (CTA→CTG)  yhhZ → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,580,1380AG83.6% 575.1 / 106.2 250L215L (CTA→CTG) yhhZhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (15/26);  new base G (62/147);  total (77/173)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-01

Only 100 of 254 total aligned reads displayed.

GCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTT  >  NC_000913/3580046‑3580229
                                                                                            |                                                                                           
  tggtggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTgaa                                                                                           >  2:2501684/1‑93 (MQ=255)
   ggtggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAAt                                                                                          <  1:169172/93‑1 (MQ=255)
    gtggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATg                                                                                         >  1:4799190/1‑93 (MQ=255)
    gtggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATg                                                                                         <  1:3927714/93‑1 (MQ=255)
    gtggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATg                                                                                         <  1:3147208/93‑1 (MQ=255)
     tggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGa                                                                                        <  1:1394915/93‑1 (MQ=255)
      ggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACtgaatgaa                                                                                       <  1:5024687/93‑1 (MQ=255)
      ggCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACtgaatgaa                                                                                       >  1:3304905/1‑93 (MQ=255)
       gCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGaaa                                                                                      <  2:2192877/93‑1 (MQ=255)
        cGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGaaaa                                                                                     <  1:5465995/93‑1 (MQ=255)
         gTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGaaaaa                                                                                    >  2:5973440/1‑93 (MQ=255)
         gTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGaaaaa                                                                                    <  1:3975755/93‑1 (MQ=255)
         gTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGaaaaa                                                                                    >  2:2656488/1‑93 (MQ=255)
          tataCAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGaaaaaa                                                                                   >  2:2118294/1‑93 (MQ=255)
           ataCAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAAc                                                                                  <  1:7469576/93‑1 (MQ=255)
            taCAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACa                                                                                 >  2:5342689/1‑93 (MQ=255)
             aCAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAg                                                                                <  2:3739156/93‑1 (MQ=255)
              cAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGc                                                                               <  1:2980085/93‑1 (MQ=255)
              cAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGc                                                                               <  1:6598374/93‑1 (MQ=255)
               aaTGGGAACATTGAAGGATTCAGAGATATTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCg                                                                              <  2:4844210/93‑1 (MQ=255)
               aaTGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCg                                                                              <  2:4154158/93‑1 (MQ=255)
               aaTGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCg                                                                              <  2:5162133/93‑1 (MQ=255)
                aTGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCgg                                                                             <  1:6803993/93‑1 (MQ=255)
                   ggAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCa                                                                          >  1:7107657/1‑93 (MQ=255)
                   ggAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCa                                                                          <  1:4605782/93‑1 (MQ=255)
                     aaCATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATa                                                                        <  2:5379402/93‑1 (MQ=255)
                     aaCATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATa                                                                        <  1:1568436/93‑1 (MQ=255)
                     aaCATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATa                                                                        <  2:4324463/93‑1 (MQ=255)
                      aCATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAg                                                                       <  1:617860/93‑1 (MQ=255)
                       cATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGc                                                                      >  1:3753911/1‑93 (MQ=255)
                         ttGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGa                                                                    <  2:2850770/93‑1 (MQ=255)
                         ttGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGa                                                                    >  2:1495589/1‑93 (MQ=255)
                         ttGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGa                                                                    <  2:4439443/93‑1 (MQ=255)
                          tGAAGGA‑TCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATa                                                                  <  1:2354260/93‑1 (MQ=255)
                           gAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATa                                                                  <  1:7344738/93‑1 (MQ=255)
                            aaGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAg                                                                 <  1:2824621/93‑1 (MQ=255)
                            aaGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAg                                                                 >  1:450681/1‑93 (MQ=255)
                              ggATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCa                                                               >  2:7352208/1‑93 (MQ=255)
                               gATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCaa                                                              <  2:567405/93‑1 (MQ=255)
                                aTTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAAc                                                             <  1:1924275/93‑1 (MQ=255)
                                  tCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAg                                                           <  1:4923784/93‑1 (MQ=255)
                                  tCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAg                                                           >  1:6970796/1‑93 (MQ=255)
                                   cAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGc                                                          <  2:6285385/93‑1 (MQ=255)
                                    agagATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCa                                                         <  1:3648305/93‑1 (MQ=255)
                                     gagaTACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCAt                                                        >  2:770722/1‑93 (MQ=255)
                                     gagaTACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCAt                                                        <  1:967579/93‑1 (MQ=255)
                                      agaTACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATc                                                       <  2:1676485/93‑1 (MQ=255)
                                      agaTACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATc                                                       <  1:5192252/93‑1 (MQ=255)
                                      agaTACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATc                                                       <  2:7264834/93‑1 (MQ=255)
                                      agaTACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATc                                                       <  1:6037978/93‑1 (MQ=255)
                                      agaTACTGCGGAAAAACTGGATGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATc                                                       <  1:5776754/93‑1 (MQ=255)
                                        aTACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTa                                                     <  1:2769888/93‑1 (MQ=255)
                                         tACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTAt                                                    <  2:62268/93‑1 (MQ=255)
                                         tACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTAt                                                    >  2:1880317/1‑93 (MQ=255)
                                         tACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGC‑GGCATAGCGATAGCAACAGCATCTAtt                                                   <  2:6928792/93‑1 (MQ=255)
                                          aCTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTAtt                                                   <  2:5610131/93‑1 (MQ=255)
                                           cTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTc                                                  <  1:268756/93‑1 (MQ=255)
                                              cGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCttt                                               <  1:220373/93‑1 (MQ=255)
                                               ggAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCtttt                                              >  2:1928336/1‑93 (MQ=255)
                                                 aaaaaCTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTaa                                            <  2:7471761/93‑1 (MQ=255)
                                                     aCTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACaaa                                        <  1:6144917/93‑1 (MQ=255)
                                                      cTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAg                                       <  1:5500391/93‑1 (MQ=255)
                                                       tGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGc                                      <  2:2327412/93‑1 (MQ=255)
                                                       tGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGc                                      <  2:4013519/93‑1 (MQ=255)
                                                       tGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGc                                      <  2:1270365/93‑1 (MQ=255)
                                                        gggTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCg                                     <  2:2968618/93‑1 (MQ=255)
                                                           tGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTc                                  <  2:1131982/93‑1 (MQ=255)
                                                           tGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTc                                  <  2:6719408/93‑1 (MQ=255)
                                                           tGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTc                                  >  2:1395628/1‑93 (MQ=255)
                                                            ggAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCt                                 <  2:3287297/93‑1 (MQ=255)
                                                               gaTGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAt                              >  1:3408018/1‑93 (MQ=255)
                                                               gaTGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAt                              >  1:718842/1‑93 (MQ=255)
                                                               gaTGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAt                              >  1:1244257/1‑93 (MQ=255)
                                                                aTGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATg                             >  2:914919/1‑93 (MQ=255)
                                                                aTGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATg                             >  2:2255411/1‑93 (MQ=255)
                                                                aTGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATg                             <  1:3674960/93‑1 (MQ=255)
                                                                 tGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGt                            <  2:3313397/93‑1 (MQ=255)
                                                                 tGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGt                            >  2:1765203/1‑93 (MQ=255)
                                                                 tGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGt                            >  2:1297165/1‑93 (MQ=255)
                                                                 tGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGt                            >  1:6250328/1‑93 (MQ=255)
                                                                 tGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGt                            >  1:6310884/1‑93 (MQ=255)
                                                                  gCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGtt                           <  2:513409/93‑1 (MQ=255)
                                                                  gCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGtt                           <  2:7003165/93‑1 (MQ=255)
                                                                   cAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTg                          <  2:4584817/93‑1 (MQ=255)
                                                                      tAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATa                       <  1:2656488/93‑1 (MQ=255)
                                                                      tAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATa                       >  2:6509441/1‑93 (MQ=255)
                                                                        aaaGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACa                     >  1:6666571/1‑93 (MQ=255)
                                                                            gCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACAtata                 <  1:1073386/93‑1 (MQ=255)
                                                                             cTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATAc                >  1:763112/1‑93 (MQ=255)
                                                                             cTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATAc                <  2:2290695/93‑1 (MQ=255)
                                                                               aTGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAg              <  1:4274446/93‑1 (MQ=255)
                                                                                 gATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGaa            >  2:4965007/1‑93 (MQ=255)
                                                                                  aTCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGaaa           <  1:634658/93‑1 (MQ=255)
                                                                                    cAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGaaata         <  1:557868/93‑1 (MQ=255)
                                                                                     aaaTACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGaaataa        <  2:360883/93‑1 (MQ=255)
                                                                                       aTACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGaaataaat      <  2:1071325/93‑1 (MQ=255)
                                                                                        tACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAaataaata     <  1:5919480/93‑1 (MQ=255)
                                                                                        tACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAaataaata     <  1:5266688/93‑1 (MQ=255)
                                                                                        tACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAaataaata     <  1:4100093/93‑1 (MQ=255)
                                                                                         aCTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAg    <  1:7358826/93‑1 (MQ=255)
                                                                                            |                                                                                           
GCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTT  >  NC_000913/3580046‑3580229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTAC  >  NC_000913/3580045‑3580231
                                                                                             |                                                                                             
TGCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTG                                                                                               <  SRR4302542.2361654/94‑1 (MQ=60)
 GCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGA                                                                                              <  SRR4302542.3210866/94‑1 (MQ=60)
 GCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGA                                                                                              >  SRR4302542.7435514/1‑94 (MQ=60)
  CTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAA                                                                                             <  SRR4302542.5157459/94‑1 (MQ=60)
    GGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATG                                                                                           >  SRR4302542.4845943/1‑94 (MQ=60)
    GGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATG                                                                                           <  SRR4302542.170602/94‑1 (MQ=60)
    GGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATG                                                                                           <  SRR4302542.4521844/94‑1 (MQ=60)
     GTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGA                                                                                          <  SRR4302542.3177102/94‑1 (MQ=60)
     GTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGA                                                                                          <  SRR4302542.3966470/94‑1 (MQ=60)
     GTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGA                                                                                          <  SRR4302542.4327449/94‑1 (MQ=60)
      TGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAA                                                                                         >  SRR4302542.3336072/1‑94 (MQ=60)
      TGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAA                                                                                         <  SRR4302542.6841111/94‑1 (MQ=60)
      TGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAA                                                                                         <  SRR4302542.1406963/94‑1 (MQ=60)
       GGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAA                                                                                        <  SRR4302542.5073461/94‑1 (MQ=60)
         CGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAA                                                                                      <  SRR4302542.5519091/94‑1 (MQ=60)
         CGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAA                                                                                      <  SRR4302542.3998101/94‑1 (MQ=60)
          GTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAA                                                                                     <  SRR4302542.4014982/94‑1 (MQ=60)
           TATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAAC                                                                                    <  SRR4302542.670278/94‑1 (MQ=60)
            ATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACA                                                                                   <  SRR4302542.7542766/94‑1 (MQ=60)
              ACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGC                                                                                 >  SRR4302542.4726218/1‑94 (MQ=60)
               CAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCG                                                                                >  SRR4302542.1680008/1‑94 (MQ=60)
               CAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCG                                                                                <  SRR4302542.5555792/94‑1 (MQ=60)
               CAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCG                                                                                <  SRR4302542.3008617/94‑1 (MQ=60)
               CAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCG                                                                                <  SRR4302542.6663383/94‑1 (MQ=60)
                AATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGG                                                                               <  SRR4302542.2301643/94‑1 (MQ=60)
                 ATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGG                                                                              <  SRR4302542.6870892/94‑1 (MQ=60)
                 ATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGG                                                                              <  SRR4302542.2042057/94‑1 (MQ=60)
                  TGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGC                                                                             <  SRR4302542.2587266/94‑1 (MQ=60)
                  TGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGC                                                                             <  SRR4302542.5059003/94‑1 (MQ=60)
                   GGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCA                                                                            >  SRR4302542.7177422/1‑94 (MQ=60)
                    GGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCAT                                                                           <  SRR4302542.4650794/94‑1 (MQ=60)
                      AACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAG                                                                         <  SRR4302542.3387296/94‑1 (MQ=60)
                      AACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAG                                                                         >  SRR4302542.4037979/1‑94 (MQ=60)
                      AACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAG                                                                         <  SRR4302542.1179077/94‑1 (MQ=60)
                      AACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAG                                                                         <  SRR4302542.1581978/94‑1 (MQ=60)
                       ACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGC                                                                        <  SRR4302542.623174/94‑1 (MQ=60)
                       ACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGC                                                                        >  SRR4302542.3791050/1‑94 (MQ=60)
                         ATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGA                                                                      >  SRR4302542.2800349/1‑94 (MQ=60)
                         ATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGA                                                                      >  SRR4302542.6229813/1‑94 (MQ=60)
                          TTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGAT                                                                     >  SRR4302542.7525887/1‑94 (MQ=60)
                           TGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATA                                                                    <  SRR4302542.7098509/94‑1 (MQ=60)
                           TGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATA                                                                    >  SRR4302542.6461590/1‑94 (MQ=60)
                           TGAAGGA‑TCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAG                                                                   <  SRR4302542.2374482/94‑1 (MQ=60)
                            GAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAG                                                                   <  SRR4302542.7416672/94‑1 (MQ=60)
                            GAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAG                                                                   >  SRR4302542.454573/1‑94 (MQ=60)
                             AAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGC                                                                  <  SRR4302542.2851773/94‑1 (MQ=60)
                                GATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAAC                                                               <  SRR4302542.7543487/94‑1 (MQ=60)
                                GATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAAC                                                               <  SRR4302542.4704664/94‑1 (MQ=60)
                                GATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAAC                                                               <  SRR4302542.4991067/94‑1 (MQ=60)
                                GATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAAC                                                               <  SRR4302542.7317002/94‑1 (MQ=60)
                                 ATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACA                                                              <  SRR4302542.1940895/94‑1 (MQ=60)
                                  TTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAG                                                             <  SRR4302542.3925423/94‑1 (MQ=60)
                                  TTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAG                                                             <  SRR4302542.2692865/94‑1 (MQ=60)
                                  TTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAG                                                             >  SRR4302542.7039271/1‑94 (MQ=60)
                                   TCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGC                                                            <  SRR4302542.4971647/94‑1 (MQ=60)
                                    CAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCA                                                           <  SRR4302542.5137593/94‑1 (MQ=60)
                                     AGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCAT                                                          <  SRR4302542.4601456/94‑1 (MQ=60)
                                     AGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCAT                                                          <  SRR4302542.3682371/94‑1 (MQ=60)
                                      GAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATC                                                         <  SRR4302542.975890/94‑1 (MQ=60)
                                      GAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATC                                                         <  SRR4302542.1374291/94‑1 (MQ=60)
                                       AGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCT                                                        <  SRR4302542.5242692/94‑1 (MQ=60)
                                       AGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCT                                                        <  SRR4302542.6096426/94‑1 (MQ=60)
                                       AGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCT                                                        >  SRR4302542.5748140/1‑94 (MQ=60)
                                       AGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCACAGCGATAGCAACAGCATCT                                                        >  SRR4302542.3581866/1‑94 (MQ=60)
                                       AGATACTGCGGAAAAACTGGATGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCT                                                        <  SRR4302542.5832830/94‑1 (MQ=60)
                                         ATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTAT                                                      <  SRR4302542.2796577/94‑1 (MQ=60)
                                          TACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATT                                                     <  SRR4302542.3687092/94‑1 (MQ=60)
                                           ACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTC                                                    >  SRR4302542.5624172/1‑94 (MQ=60)
                                            CTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCT                                                   <  SRR4302542.271045/94‑1 (MQ=60)
                                              GCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTT                                                 >  SRR4302542.7122459/1‑94 (MQ=60)
                                               CGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTT                                                <  SRR4302542.7352102/94‑1 (MQ=60)
                                               CGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTT                                                <  SRR4302542.7185302/94‑1 (MQ=60)
                                               CGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTT                                                <  SRR4302542.222275/94‑1 (MQ=60)
                                                     AACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAA                                          <  SRR4302542.591749/94‑1 (MQ=60)
                                                     AACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAA                                          <  SRR4302542.774327/94‑1 (MQ=60)
                                                      ACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAG                                         <  SRR4302542.6204371/94‑1 (MQ=60)
                                                      ACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAG                                         <  SRR4302542.660440/94‑1 (MQ=60)
                                                       CTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGC                                        <  SRR4302542.5553818/94‑1 (MQ=60)
                                                        TGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCG                                       <  SRR4302542.2029958/94‑1 (MQ=60)
                                                           GTGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTC                                    <  SRR4302542.6263548/94‑1 (MQ=60)
                                                            TGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCT                                   <  SRR4302542.6138700/94‑1 (MQ=60)
                                                            TGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCT                                   >  SRR4302542.4859353/1‑94 (MQ=60)
                                                            TGGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCT                                   <  SRR4302542.554316/94‑1 (MQ=60)
                                                             GGAGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTA                                  <  SRR4302542.4963610/94‑1 (MQ=60)
                                                               AGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAT                                <  SRR4302542.2910117/94‑1 (MQ=60)
                                                               AGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAT                                >  SRR4302542.3439964/1‑94 (MQ=60)
                                                               AGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAT                                >  SRR4302542.725037/1‑94 (MQ=60)
                                                               AGATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAT                                >  SRR4302542.1910828/1‑94 (MQ=60)
                                                               AGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAAT                                >  SRR4302542.1254952/1‑94 (MQ=60)
                                                                GATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATG                               <  SRR4302542.683296/94‑1 (MQ=60)
                                                                 ATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGT                              >  SRR4302542.6373066/1‑94 (MQ=60)
                                                                 ATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGT                              <  SRR4302542.4030033/94‑1 (MQ=60)
                                                                 ATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGT                              >  SRR4302542.6311913/1‑94 (MQ=60)
                                                                 ATGCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGT                              <  SRR4302542.3710563/94‑1 (MQ=60)
                                                                   GCAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTG                            <  SRR4302542.2738821/94‑1 (MQ=60)
                                                                    CAATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGA                           >  SRR4302542.3175334/1‑94 (MQ=60)
                                                                    CAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGA                           <  SRR4302542.6129075/94‑1 (MQ=60)
                                                                     AATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGAT                          >  SRR4302542.1360681/1‑94 (MQ=60)
                                                                      ATAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATA                         >  SRR4302542.5473128/1‑94 (MQ=60)
                                                                       TAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATAC                        <  SRR4302542.6031252/94‑1 (MQ=60)
                                                                       TAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATAC                        <  SRR4302542.2682132/94‑1 (MQ=60)
                                                                       TAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATAC                        <  SRR4302542.5281123/94‑1 (MQ=60)
                                                                       TAAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATAC                        <  SRR4302542.153254/94‑1 (MQ=60)
                                                                        AAAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACA                       >  SRR4302542.6732251/1‑94 (MQ=60)
                                                                        AAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACA                       >  SRR4302542.6242045/1‑94 (MQ=60)
                                                                         AAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACAT                      <  SRR4302542.3949990/94‑1 (MQ=60)
                                                                         AAAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACAT                      <  SRR4302542.3226359/94‑1 (MQ=60)
                                                                          AAGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATA                     <  SRR4302542.7457881/94‑1 (MQ=60)
                                                                           AGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATAT                    >  SRR4302542.5142785/1‑94 (MQ=60)
                                                                           AGGCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATAT                    <  SRR4302542.5380333/94‑1 (MQ=60)
                                                                             GCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATAC                  <  SRR4302542.1082610/94‑1 (MQ=60)
                                                                             GCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATAC                  <  SRR4302542.2732517/94‑1 (MQ=60)
                                                                             GCTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATAC                  >  SRR4302542.769720/1‑94 (MQ=60)
                                                                              CTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACA                 >  SRR4302542.3690811/1‑94 (MQ=60)
                                                                              CTATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACA                 >  SRR4302542.3018012/1‑94 (MQ=60)
                                                                               TATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAG                >  SRR4302542.6357367/1‑94 (MQ=60)
                                                                                ATGATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGA               <  SRR4302542.4316497/94‑1 (MQ=60)
                                                                                  GATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAA             >  SRR4302542.3410511/1‑94 (MQ=60)
                                                                                  GATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAA             >  SRR4302542.1610968/1‑94 (MQ=60)
                                                                                   ATCAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAAT            <  SRR4302542.640124/94‑1 (MQ=60)
                                                                                     CAAATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAA          <  SRR4302542.4257310/94‑1 (MQ=60)
                                                                                     CAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAA          <  SRR4302542.562674/94‑1 (MQ=60)
                                                                                       AATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAAT        <  SRR4302542.258649/94‑1 (MQ=60)
                                                                                       AATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAAT        <  SRR4302542.741554/94‑1 (MQ=60)
                                                                                       AATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAAT        >  SRR4302542.3750208/1‑94 (MQ=60)
                                                                                        ATACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATA       >  SRR4302542.6384092/1‑94 (MQ=60)
                                                                                        ATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATA       >  SRR4302542.2929752/1‑94 (MQ=60)
                                                                                         TACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAG      <  SRR4302542.5317869/94‑1 (MQ=60)
                                                                                         TACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAG      <  SRR4302542.5976842/94‑1 (MQ=60)
                                                                                         TACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAG      <  SRR4302542.4140525/94‑1 (MQ=60)
                                                                                         TACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAG      <  SRR4302542.7013070/94‑1 (MQ=60)
                                                                                         TACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAG      <  SRR4302542.6338078/94‑1 (MQ=60)
                                                                                          ACTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGT     <  SRR4302542.7430907/94‑1 (MQ=60)
                                                                                           CTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTT    >  SRR4302542.5454040/1‑94 (MQ=60)
                                                                                           CTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTT    <  SRR4302542.5460443/94‑1 (MQ=60)
                                                                                           CTGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTT    >  SRR4302542.7071867/1‑94 (MQ=60)
                                                                                           CTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTT    <  SRR4302542.5861904/94‑1 (MQ=60)
                                                                                            TGAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTA   <  SRR4302542.3576102/94‑1 (MQ=60)
                                                                                             GAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTAC  <  SRR4302542.4196304/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTAC  >  NC_000913/3580045‑3580231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑