Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,609,245 T→C L54L (TTA→TTG)  yhhS ← putative transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,609,2450TC84.1% 698.3 / 111.2 303L54L (TTA→TTG) yhhSputative transport
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base C (120/134);  total (146/157)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 304 total aligned reads displayed.

GCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAG  >  NC_000913/3609155‑3609337
                                                                                          |                                                                                            
gcggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCaa                                                                                            <  2:4606864/93‑1 (MQ=255)
 cggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAAt                                                                                           >  1:3138532/1‑93 (MQ=255)
  ggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATa                                                                                          <  1:3817807/93‑1 (MQ=255)
  ggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATa                                                                                          <  1:744409/93‑1 (MQ=255)
  ggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATa                                                                                          >  1:2257230/1‑93 (MQ=255)
  ggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATa                                                                                          >  1:1359950/1‑93 (MQ=255)
  ggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATa                                                                                          >  1:4472114/1‑93 (MQ=255)
   gcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATAc                                                                                         <  1:195342/93‑1 (MQ=255)
     tCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACag                                                                                       <  2:2313247/93‑1 (MQ=255)
     tCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACag                                                                                       >  1:958873/1‑93 (MQ=255)
     tCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACag                                                                                       <  1:1041152/93‑1 (MQ=255)
      cAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACagc                                                                                      >  1:1577685/1‑93 (MQ=255)
      cAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACagc                                                                                      <  2:2545008/93‑1 (MQ=255)
          aaGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACagcgagc                                                                                  >  1:1719784/1‑93 (MQ=255)
          aaGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACagcgagc                                                                                  <  2:1959552/93‑1 (MQ=255)
           aGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACagcgagcg                                                                                 <  1:436255/93‑1 (MQ=255)
           aGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACagcgagcg                                                                                 <  2:2757011/93‑1 (MQ=255)
            ngTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCng                                                                                >  2:5951273/2‑93 (MQ=255)
             gTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGc                                                                               <  2:1878101/93‑1 (MQ=255)
                gCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAAc                                                                            <  2:1927127/93‑1 (MQ=255)
                 cGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAAcc                                                                           <  2:1686197/93‑1 (MQ=255)
                  gAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAAccc                                                                          <  2:87101/93‑1 (MQ=255)
                  gAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAAccc                                                                          <  1:3978118/93‑1 (MQ=255)
                  gAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAAccc                                                                          >  1:83058/1‑93 (MQ=255)
                  gAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAAccc                                                                          <  2:1147255/93‑1 (MQ=255)
                  gAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAAccc                                                                          >  2:4934347/1‑93 (MQ=255)
                    aaTATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGa                                                                        >  2:655011/1‑93 (MQ=255)
                     atatTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGAt                                                                       <  1:3688094/93‑1 (MQ=255)
                       atTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATgg                                                                     >  2:2955017/1‑93 (MQ=255)
                          gCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGa                                                                  >  2:80030/1‑93 (MQ=255)
                          gCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGa                                                                  >  1:2577782/1‑93 (MQ=255)
                            aGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAgg                                                                <  1:4118442/93‑1 (MQ=255)
                            aGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAgg                                                                >  2:1341319/1‑93 (MQ=255)
                            aGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAgg                                                                <  1:6072593/93‑1 (MQ=255)
                            aGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAgg                                                                <  1:2063974/93‑1 (MQ=255)
                             ggCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGt                                                               <  1:3028533/93‑1 (MQ=255)
                              gCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTa                                                              <  2:1481534/93‑1 (MQ=255)
                                tGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGc                                                            >  1:1074199/1‑93 (MQ=255)
                                tGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGc                                                            <  2:2839836/93‑1 (MQ=255)
                                tGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGc                                                            >  2:1310431/1‑93 (MQ=255)
                                 gATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCt                                                           >  2:2058236/1‑93 (MQ=255)
                                 gATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCt                                                           >  2:5767805/1‑93 (MQ=255)
                                  aNAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTg                                                          <  2:6662184/93‑1 (MQ=255)
                                    aaCCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGc                                                        <  2:3667377/93‑1 (MQ=255)
                                     aCCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCg                                                       >  2:5054839/1‑93 (MQ=255)
                                      ccAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGa                                                      <  2:7194502/93‑1 (MQ=255)
                                      ccAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGa                                                      >  2:3435133/1‑93 (MQ=255)
                                        aaTCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAg                                                    >  1:4802868/1‑93 (MQ=255)
                                        aaTCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAg                                                    <  1:1458129/93‑1 (MQ=255)
                                         aTCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGt                                                   <  1:6624658/93‑1 (MQ=255)
                                          tCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGtt                                                  <  2:1801099/93‑1 (MQ=255)
                                           ccTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTa                                                 <  2:220325/93‑1 (MQ=255)
                                             tGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTaaa                                               <  2:6879498/93‑1 (MQ=255)
                                             tGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTaaa                                               >  1:1231049/1‑93 (MQ=255)
                                             tGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTaaa                                               <  1:5813549/93‑1 (MQ=255)
                                               cccAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACa                                             >  2:14588/1‑93 (MQ=255)
                                               cccAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACa                                             <  1:7334127/93‑1 (MQ=255)
                                                ccAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                            <  2:3507211/93‑1 (MQ=255)
                                                ccAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                            >  1:5002566/1‑93 (MQ=255)
                                                ccAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                            <  1:6653818/93‑1 (MQ=255)
                                                ccAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                            <  1:5133873/93‑1 (MQ=255)
                                                ccAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                            <  1:3923356/93‑1 (MQ=255)
                                                 cAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATg                                           >  1:454591/1‑93 (MQ=255)
                                                  aGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGa                                          >  2:223961/1‑93 (MQ=255)
                                                   gAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGAc                                         <  1:7424721/93‑1 (MQ=255)
                                                      ggCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACtat                                      <  1:4274171/93‑1 (MQ=255)
                                                      ggCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACtat                                      <  2:4828069/93‑1 (MQ=255)
                                                       gcgcTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACtata                                     <  1:2999961/93‑1 (MQ=255)
                                                         gcTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATaga                                   >  2:775384/1‑93 (MQ=255)
                                                         gcTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATaga                                   <  2:3360429/93‑1 (MQ=255)
                                                         gcTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATaga                                   <  2:5546362/93‑1 (MQ=255)
                                                           tAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAgaga                                 >  2:3178811/1‑93 (MQ=255)
                                                            aaaGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGAc                                <  1:1187973/93‑1 (MQ=255)
                                                            aaaGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGAc                                <  1:3355899/93‑1 (MQ=255)
                                                              aGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACaa                              <  2:4446257/93‑1 (MQ=255)
                                                                 ccATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATgc                           >  1:5485908/1‑93 (MQ=255)
                                                                   atcacatcaTGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATgcgc                         >  1:6954629/1‑93 (MQ=255)
                                                                    tcacatcaTGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCa                        >  2:4060515/1‑93 (MQ=255)
                                                                      acatcaTGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCaaa                      >  1:6525360/1‑93 (MQ=255)
                                                                        atcatGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAAtt                    <  1:2803090/93‑1 (MQ=255)
                                                                          catGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCa                  >  1:6359207/1‑93 (MQ=255)
                                                                          catGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCa                  >  2:4416020/1‑93 (MQ=255)
                                                                          catGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCa                  <  2:5234304/93‑1 (MQ=255)
                                                                          catGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCa                  <  1:3449611/93‑1 (MQ=255)
                                                                           atGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAg                 <  2:2264355/93‑1 (MQ=255)
                                                                           atGGACATAGCCCGAAAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAACCATGACTATAGAGACAATGCGCAAATTCAg                 <  1:5881934/93‑1 (MQ=255)
                                                                           atGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAg                 >  2:265687/1‑93 (MQ=255)
                                                                           atGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAg                 <  1:2519574/93‑1 (MQ=255)
                                                                            tGGACATAGCCCGGCAATACAGCGAGCGGTAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAgg                >  1:597788/1‑93 (MQ=255)
                                                                            tGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAgg                >  2:7076210/1‑93 (MQ=255)
                                                                             ggACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgc               <  2:305615/93‑1 (MQ=255)
                                                                             ggACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgc               <  1:6300152/93‑1 (MQ=255)
                                                                             ggACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgc               <  2:4564445/93‑1 (MQ=255)
                                                                              gACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgcg              >  1:7139323/1‑93 (MQ=255)
                                                                               aCATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgcgc             >  2:6776812/1‑93 (MQ=255)
                                                                                 aTAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCaa           <  1:182794/93‑1 (MQ=255)
                                                                                   aGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATc         <  2:4246564/93‑1 (MQ=255)
                                                                                    gCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATcc        <  2:7422970/93‑1 (MQ=255)
                                                                                     cccGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCg       <  1:6766861/93‑1 (MQ=255)
                                                                                       cGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGtt     <  2:3701407/93‑1 (MQ=255)
                                                                                          |                                                                                            
GCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAG  >  NC_000913/3609155‑3609337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGC  >  NC_000913/3609155‑3609338
                                                                                          |                                                                                             
GCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAAT                                                                                            >  SRR4302542.3168366/1‑94 (MQ=60)
 TGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATA                                                                                           >  SRR4302542.2276634/1‑94 (MQ=60)
 CGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATA                                                                                           >  SRR4302542.1371712/1‑94 (MQ=60)
 CGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATA                                                                                           >  SRR4302542.4515977/1‑94 (MQ=60)
  GGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATAC                                                                                          <  SRR4302542.3855566/94‑1 (MQ=60)
  GGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATAC                                                                                          <  SRR4302542.750857/94‑1 (MQ=60)
   GCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACA                                                                                         <  SRR4302542.197002/94‑1 (MQ=60)
    CTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAG                                                                                        >  SRR4302542.6687136/1‑94 (MQ=60)
    CTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAG                                                                                        >  SRR4302542.967109/1‑94 (MQ=60)
    CTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAG                                                                                        <  SRR4302542.6343628/94‑1 (MQ=60)
     TCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGC                                                                                       >  SRR4302542.1591309/1‑94 (MQ=60)
     TCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGC                                                                                       <  SRR4302542.1050090/94‑1 (MQ=60)
      CAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCG                                                                                      >  SRR4302542.3500300/1‑94 (MQ=60)
      CAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCG                                                                                      <  SRR4302542.1807779/94‑1 (MQ=60)
      CAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCG                                                                                      <  SRR4302542.3354712/94‑1 (MQ=60)
       AGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGA                                                                                     >  SRR4302542.1425930/1‑94 (MQ=60)
       AGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGA                                                                                     >  SRR4302542.1869262/1‑94 (MQ=60)
       AGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGA                                                                                     >  SRR4302542.5179174/1‑94 (MQ=60)
        GCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAG                                                                                    >  SRR4302542.745063/1‑94 (MQ=60)
         CAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGC                                                                                   >  SRR4302542.1734717/1‑94 (MQ=60)
         CAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGC                                                                                   >  SRR4302542.1989388/1‑94 (MQ=60)
          AAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCG                                                                                  >  SRR4302542.5985483/1‑94 (MQ=60)
          AAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCG                                                                                  <  SRR4302542.6885112/94‑1 (MQ=60)
           AGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGG                                                                                 <  SRR4302542.440034/94‑1 (MQ=60)
             GTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCA                                                                               <  SRR4302542.1200117/94‑1 (MQ=60)
               GGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAAC                                                                             >  SRR4302542.1400976/1‑94 (MQ=60)
                GCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACC                                                                            >  SRR4302542.1076428/1‑94 (MQ=60)
                 CGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCC                                                                           >  SRR4302542.959648/1‑94 (MQ=60)
                 CGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCC                                                                           >  SRR4302542.83752/1‑94 (MQ=60)
                 CGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCC                                                                           <  SRR4302542.1704338/94‑1 (MQ=60)
                  GAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCG                                                                          <  SRR4302542.4017370/94‑1 (MQ=60)
                  GAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCG                                                                          >  SRR4302542.4524785/1‑94 (MQ=60)
                  GAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCG                                                                          >  SRR4302542.7407071/1‑94 (MQ=60)
                     ATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATG                                                                       >  SRR4302542.7215592/1‑94 (MQ=60)
                     ATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATG                                                                       <  SRR4302542.3724544/94‑1 (MQ=60)
                       ATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGT                                                                     >  SRR4302542.183714/1‑94 (MQ=60)
                        TTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTG                                                                    <  SRR4302542.5684308/94‑1 (MQ=60)
                        TTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTG                                                                    <  SRR4302542.5283350/94‑1 (MQ=60)
                         TGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGA                                                                   >  SRR4302542.2602772/1‑94 (MQ=60)
                          GCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAG                                                                  <  SRR4302542.2927297/94‑1 (MQ=60)
                           CAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGG                                                                 >  SRR4302542.1063900/1‑94 (MQ=60)
                           CAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGG                                                                 <  SRR4302542.466389/94‑1 (MQ=60)
                            AGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGT                                                                >  SRR4302542.7152273/1‑94 (MQ=60)
                            AGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGT                                                                <  SRR4302542.6131407/94‑1 (MQ=60)
                            AGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGT                                                                <  SRR4302542.4159015/94‑1 (MQ=60)
                            AGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGT                                                                <  SRR4302542.2081816/94‑1 (MQ=60)
                             GGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTA                                                               >  SRR4302542.6435067/1‑94 (MQ=60)
                             GGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTA                                                               <  SRR4302542.3057438/94‑1 (MQ=60)
                              GCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAG                                                              >  SRR4302542.5501087/1‑94 (MQ=60)
                               CTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGC                                                             >  SRR4302542.1083435/1‑94 (MQ=60)
                               CTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGC                                                             >  SRR4302542.4284828/1‑94 (MQ=60)
                               CTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGC                                                             <  SRR4302542.5887559/94‑1 (MQ=60)
                                TGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCT                                                            >  SRR4302542.3107574/1‑94 (MQ=60)
                                TGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCT                                                            >  SRR4302542.2805133/1‑94 (MQ=60)
                                TGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCT                                                            <  SRR4302542.6090970/94‑1 (MQ=60)
                                  ATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGG                                                          <  SRR4302542.485237/94‑1 (MQ=60)
                                  ATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGG                                                          >  SRR4302542.406327/1‑94 (MQ=60)
                                  ATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGG                                                          <  SRR4302542.1517602/94‑1 (MQ=60)
                                  ATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGG                                                          <  SRR4302542.2234054/94‑1 (MQ=60)
                                   TAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGC                                                         >  SRR4302542.6085141/1‑94 (MQ=60)
                                    AACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCG                                                        >  SRR4302542.1168571/1‑94 (MQ=60)
                                       CAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG                                                     >  SRR4302542.4849654/1‑94 (MQ=60)
                                       CAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG                                                     <  SRR4302542.1917354/94‑1 (MQ=60)
                                       CAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG                                                     >  SRR4302542.7555953/1‑94 (MQ=60)
                                       CAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG                                                     <  SRR4302542.5700667/94‑1 (MQ=60)
                                       CAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG                                                     >  SRR4302542.885490/1‑94 (MQ=60)
                                        AATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGT                                                    >  SRR4302542.345548/1‑94 (MQ=60)
                                        AATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGT                                                    <  SRR4302542.1230422/94‑1 (MQ=60)
                                        AATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGT                                                    <  SRR4302542.1470713/94‑1 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   >  SRR4302542.4160942/1‑94 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   <  SRR4302542.4117165/94‑1 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   >  SRR4302542.4662847/1‑94 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   <  SRR4302542.6689973/94‑1 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   <  SRR4302542.4365848/94‑1 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   >  SRR4302542.2539586/1‑94 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   <  SRR4302542.6690299/94‑1 (MQ=60)
                                         ATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTT                                                   >  SRR4302542.2520320/1‑94 (MQ=60)
                                            CTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAA                                                >  SRR4302542.1241636/1‑94 (MQ=60)
                                            CTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAA                                                >  SRR4302542.6032377/1‑94 (MQ=60)
                                             TGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAAC                                               <  SRR4302542.2134547/94‑1 (MQ=60)
                                             TGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAAC                                               <  SRR4302542.5869963/94‑1 (MQ=60)
                                             TGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAAC                                               <  SRR4302542.7524925/94‑1 (MQ=60)
                                             TGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAAC                                               >  SRR4302542.1104582/1‑94 (MQ=60)
                                             TGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAAC                                               <  SRR4302542.6353217/94‑1 (MQ=60)
                                              GCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACA                                              <  SRR4302542.3950660/94‑1 (MQ=60)
                                              GCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACA                                              <  SRR4302542.3297778/94‑1 (MQ=60)
                                               CCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGGGAGCGGCAACCCGATGGAGAGGAAGCTAGCGAAGCTAAACAT                                             >  SRR4302542.2608248/1‑94 (MQ=60)
                                               CCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAT                                             >  SRR4302542.3733067/1‑94 (MQ=60)
                                               CCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAT                                             >  SRR4302542.5051159/1‑94 (MQ=60)
                                               CCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAT                                             <  SRR4302542.7405975/94‑1 (MQ=60)
                                               CCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAT                                             >  SRR4302542.6403477/1‑94 (MQ=60)
                                                CCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATG                                            <  SRR4302542.6719381/94‑1 (MQ=60)
                                                CCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATG                                            <  SRR4302542.5183778/94‑1 (MQ=60)
                                                CCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATG                                            >  SRR4302542.458517/1‑94 (MQ=60)
                                                CCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATG                                            <  SRR4302542.3962068/94‑1 (MQ=60)
                                                CCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATG                                            >  SRR4302542.7326892/1‑94 (MQ=60)
                                                  AGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGAC                                          >  SRR4302542.6701963/1‑94 (MQ=60)
                                                   GAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACT                                         <  SRR4302542.7497378/94‑1 (MQ=60)
                                                    AAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTA                                        >  SRR4302542.3128513/1‑94 (MQ=60)
                                                     AGGCGCTAAACCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTAT                                       <  SRR4302542.4219055/94‑1 (MQ=60)
                                                      GGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATA                                      <  SRR4302542.4518801/94‑1 (MQ=60)
                                                      GGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATA                                      <  SRR4302542.1312601/94‑1 (MQ=60)
                                                      GGCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATA                                      <  SRR4302542.4316220/94‑1 (MQ=60)
                                                       GCGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAG                                     <  SRR4302542.4414967/94‑1 (MQ=60)
                                                       GCGCTAAAGCCCATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAG                                     <  SRR4302542.3028657/94‑1 (MQ=60)
                                                        CGCTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGA                                    >  SRR4302542.2051568/1‑94 (MQ=60)
                                                          CTAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGA                                  >  SRR4302542.124801/1‑94 (MQ=60)
                                                           TAAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGAC                                 >  SRR4302542.6823789/1‑94 (MQ=60)
                                                            AAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACA                                <  SRR4302542.3387459/94‑1 (MQ=60)
                                                            AAAGCCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACA                                <  SRR4302542.1198211/94‑1 (MQ=60)
                                                                CCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGC                            >  SRR4302542.5886165/1‑94 (MQ=60)
                                                                CCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGC                            >  SRR4302542.5539199/1‑94 (MQ=60)
                                                                CCCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGC                            >  SRR4302542.3030459/1‑94 (MQ=60)
                                                                 CCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCG                           <  SRR4302542.4881953/94‑1 (MQ=60)
                                                                 CCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCG                           <  SRR4302542.348329/94‑1 (MQ=60)
                                                                 CCATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCG                           >  SRR4302542.3965475/1‑94 (MQ=60)
                                                                  CATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGC                          >  SRR4302542.397044/1‑94 (MQ=60)
                                                                  CATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGC                          >  SRR4302542.7022958/1‑94 (MQ=60)
                                                                   ATCACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCA                         <  SRR4302542.3930914/94‑1 (MQ=60)
                                                                   ATCACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCA                         <  SRR4302542.3056449/94‑1 (MQ=60)
                                                                     CACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAA                       >  SRR4302542.6589662/1‑94 (MQ=60)
                                                                     CACATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGCGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAA                       >  SRR4302542.2934270/1‑94 (MQ=60)
                                                                      ACATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAAT                      <  SRR4302542.155187/94‑1 (MQ=60)
                                                                        ATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTC                    <  SRR4302542.2830079/94‑1 (MQ=60)
                                                                        ATCATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGCAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTC                    >  SRR4302542.6594844/1‑94 (MQ=60)
                                                                        ATCATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTC                    >  SRR4302542.505363/1‑94 (MQ=60)
                                                                         TCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCA                   >  SRR4302542.6421834/1‑94 (MQ=60)
                                                                          CATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAG                  <  SRR4302542.3481887/94‑1 (MQ=60)
                                                                           ATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 >  SRR4302542.7507503/1‑94 (MQ=60)
                                                                           ATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGCGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 >  SRR4302542.7507502/1‑94 (MQ=60)
                                                                           ATGGACATAGCCCGGCAATACAGCGAGCGGTAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 >  SRR4302542.602940/1‑94 (MQ=60)
                                                                           ATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 >  SRR4302542.3462921/1‑94 (MQ=60)
                                                                           ATGGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 >  SRR4302542.3786173/1‑94 (MQ=60)
                                                                           ATGGACATAGCCCGAAAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAACCATGACTATAGAGACAATGCGCAAATTCAGG                 <  SRR4302542.5938958/94‑1 (MQ=60)
                                                                           ATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 <  SRR4302542.2596817/94‑1 (MQ=60)
                                                                           ATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 <  SRR4302542.2544013/94‑1 (MQ=60)
                                                                           ATGGACATAGCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGG                 >  SRR4302542.4363090/1‑94 (MQ=60)
                                                                             GGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCG               >  SRR4302542.257397/1‑94 (MQ=60)
                                                                             GGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCG               <  SRR4302542.6529420/94‑1 (MQ=60)
                                                                             GGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCG               >  SRR4302542.7209391/1‑94 (MQ=60)
                                                                             GGACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCG               <  SRR4302542.6362220/94‑1 (MQ=60)
                                                                               ACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCA             >  SRR4302542.6818138/1‑94 (MQ=60)
                                                                               ACATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCA             <  SRR4302542.6419693/94‑1 (MQ=60)
                                                                                 ATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAAT           <  SRR4302542.184341/94‑1 (MQ=60)
                                                                                 ATAGCCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAAT           <  SRR4302542.4502121/94‑1 (MQ=60)
                                                                                    GCCCAGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCG        >  SRR4302542.2124691/1‑94 (MQ=60)
                                                                                     CCCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGT       <  SRR4302542.6833408/94‑1 (MQ=60)
                                                                                      CCGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTT      <  SRR4302542.6911183/94‑1 (MQ=60)
                                                                                       CGGCAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTT     <  SRR4302542.7114211/94‑1 (MQ=60)
                                                                                         GTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAG   <  SRR4302542.1611554/94‑1 (MQ=60)
                                                                                          CAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGC  >  SRR4302542.204382/1‑94 (MQ=60)
                                                                                          |                                                                                             
GCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGC  >  NC_000913/3609155‑3609338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑