Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,627,142 +G intergenic (‑24/+23) yhiH ← / ← yhiI putative ATP‑binding component of a transport system, fragment 1/putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,627,1401.G99.3% 962.2 / ‑2.7 289intergenic (‑22/+25)yhiH/yhiIputative ATP‑binding component of a transport system, fragment 1/putative membrane protein
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (168/119);  total (170/119)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227
                                                                                             |                                                                                        
 nnaTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                         <  1:3130587/91‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                         <  2:2407104/93‑1 (MQ=255)
   aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggg                                                                                       >  2:4698706/1‑92 (MQ=255)
    tACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggga                                                                                      >  1:4044259/1‑91 (MQ=255)
    tACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggga                                                                                      <  2:7101189/93‑3 (MQ=255)
    tACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggga                                                                                      <  2:6162081/93‑3 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACggg                                                                                  >  1:3502185/1‑93 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACggg                                                                                  >  1:2775505/1‑93 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACggg                                                                                  >  2:2576053/1‑93 (MQ=255)
          gggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGaa                                                                                >  1:5527628/1‑93 (MQ=255)
          gggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGaa                                                                                <  2:1687732/93‑1 (MQ=255)
            gCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAAcc                                                                              >  1:388348/1‑93 (MQ=255)
             ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCa                                                                             <  2:2008669/93‑1 (MQ=255)
             ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCa                                                                             >  2:1907469/1‑93 (MQ=255)
              cGGAAGATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAg                                                                            >  1:4703208/1‑93 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGt                                                                           <  1:5732715/93‑1 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGt                                                                           >  1:6851383/1‑93 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGt                                                                           >  1:2969658/1‑93 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGt                                                                           <  2:6119514/93‑1 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGtt                                                                          >  2:5068512/1‑93 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGtt                                                                          <  2:5974487/93‑1 (MQ=255)
                 aaTATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTc                                                                         <  2:6299642/93‑1 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTcc                                                                        <  2:5005393/93‑1 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCa                                                                       <  2:3052082/93‑1 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCa                                                                       <  2:654462/93‑1 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGCTCCa                                                                       >  2:1474244/1‑93 (MQ=255)
                    atCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAg                                                                      >  2:5530983/1‑93 (MQ=255)
                     tCGAGAGCGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCGGTTGCGCGACAATCGGG‑AAGGGGACCAGCTcctcc                                                                     >  1:6727431/1‑90 (MQ=255)
                      cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGAt                                                                    <  2:457138/93‑1 (MQ=255)
                       gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATg                                                                   <  2:827804/93‑1 (MQ=255)
                       gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATg                                                                   >  2:5425420/1‑93 (MQ=255)
                         gagTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCg                                                                 <  2:7386728/93‑1 (MQ=255)
                          agTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGAACGGGAACCAGTTCCAGATGCGt                                                                >  1:5979040/1‑93 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTc                                                               >  2:645128/1‑93 (MQ=255)
                             gATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAt                                                             <  2:1259080/93‑1 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAtt                                                            >  2:2717341/1‑93 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAtt                                                            <  1:649058/93‑1 (MQ=255)
                               tatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTg                                                           >  2:4873857/1‑93 (MQ=255)
                                atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGc                                                          >  2:2979114/1‑93 (MQ=255)
                                atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGc                                                          >  2:4068662/1‑93 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGGACCAGTTCCAGATGCGTCATTGCg                                                         >  1:5866779/1‑93 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         >  1:2179550/1‑93 (MQ=255)
                                   gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGc                                                       <  1:121504/93‑1 (MQ=255)
                                     tCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                     >  1:143356/1‑93 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   <  2:5727586/93‑1 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   >  2:3118137/1‑93 (MQ=255)
                                         cgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTc                                                 <  1:856097/93‑1 (MQ=255)
                                          gcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTca                                                <  1:7325486/93‑1 (MQ=255)
                                          gcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTca                                                >  2:1789582/1‑93 (MQ=255)
                                           cAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcac                                               <  2:2170436/93‑1 (MQ=255)
                                            aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacc                                              >  1:5648512/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             >  1:4909097/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             >  2:3767196/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             >  1:6953902/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             <  2:384144/93‑1 (MQ=255)
                                              cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccac                                            <  2:4411178/93‑1 (MQ=255)
                                              cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccac                                            <  2:5220249/93‑1 (MQ=255)
                                               ggTTTTTCCATAATGCTGGCTCACGCCCGCCCGTTGCGCGACAGGC‑GGAACCAGAACCAGCTCCAGATGCGTCATTGCGGCAACCTcaccacc                                           >  2:545893/1‑92 (MQ=255)
                                               ggTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                           >  1:2483070/1‑93 (MQ=255)
                                                gTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                          >  1:3553989/1‑93 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         >  1:984009/1‑93 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         >  2:2792544/1‑93 (MQ=255)
                                                  ttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg                                        >  2:3358110/1‑93 (MQ=255)
                                                  ttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg                                        >  1:7166733/1‑93 (MQ=255)
                                                   tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAggg                                       >  1:5119784/1‑92 (MQ=255)
                                                     tCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcg                                     >  1:2295065/1‑93 (MQ=255)
                                                      ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgt                                    <  2:3555326/93‑1 (MQ=255)
                                                      ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgt                                    >  1:7236672/1‑93 (MQ=255)
                                                      ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgt                                    >  2:588743/1‑93 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  <  1:3338610/93‑1 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  >  2:1093825/1‑93 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  <  2:6890647/93‑1 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  >  2:3642919/1‑93 (MQ=255)
                                                         tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                 <  2:4834267/93‑1 (MQ=255)
                                                         tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                 >  1:344625/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGc                               <  1:3943863/93‑1 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACTTCACCACGAGGTCGTCAGGcc                              >  2:2608375/1‑93 (MQ=255)
                                                              cTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAc                            <  1:443856/93‑1 (MQ=255)
                                                               tGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                           >  1:4606166/1‑93 (MQ=255)
                                                                  cTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa                        <  1:1843883/93‑1 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       <  2:1552123/93‑1 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       <  1:3798799/93‑1 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       >  2:3960301/1‑93 (MQ=255)
                                                                    cACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGt                      >  2:4129621/1‑93 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    >  1:7073853/1‑93 (MQ=255)
                                                                       gcccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttct                   <  1:3253085/93‑1 (MQ=255)
                                                                         ccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttcttc                 <  2:1185332/93‑1 (MQ=255)
                                                                          cgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                <  1:1528/93‑1 (MQ=255)
                                                                          cgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                <  2:6742609/93‑1 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               >  2:135551/1‑93 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               >  1:1326988/1‑93 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              >  1:2543164/1‑93 (MQ=255)
                                                                             cAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattc             <  2:6844542/93‑1 (MQ=255)
                                                                               gTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  1:3698034/1‑93 (MQ=255)
                                                                               gTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  2:658480/1‑93 (MQ=255)
                                                                                ttGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAcc          >  2:778040/1‑93 (MQ=255)
                                                                                  gcgcGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg        <  1:6611363/93‑1 (MQ=255)
                                                                                    gcgACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCa      <  2:5273794/93‑1 (MQ=255)
                                                                                     cgACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAc     >  1:6420800/1‑93 (MQ=255)
                                                                                      gACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAcc    <  1:1100593/93‑1 (MQ=255)
                                                                                             |                                                                                        
ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC  >  NC_000913/3627049‑3627233
                                                                                              |                                                                                            
NNATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                              <  SRR4302542.3160357/94‑1 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                              >  SRR4302542.2125697/1‑94 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                              >  SRR4302542.6791952/1‑94 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑G                                                                                            <  SRR4302542.5880001/94‑1 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                            >  SRR4302542.4084159/1‑92 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACG                                                                                          >  SRR4302542.7253887/1‑94 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGG                                                                                        <  SRR4302542.1377766/94‑1 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGG                                                                                        <  SRR4302542.1500820/94‑1 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGG                                                                                        >  SRR4302542.267486/1‑94 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGG                                                                                        >  SRR4302542.3534940/1‑94 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGG                                                                                        >  SRR4302542.2802237/1‑94 (MQ=60)
       GCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGA                                                                                       <  SRR4302542.2125329/94‑1 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAA                                                                                      >  SRR4302542.5581354/1‑94 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAA                                                                                      <  SRR4302542.5014513/94‑1 (MQ=60)
         GGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAAC                                                                                     <  SRR4302542.1682924/94‑1 (MQ=60)
          GGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACC                                                                                    >  SRR4302542.391714/1‑94 (MQ=60)
            CCGGAAGATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAG                                                                                  >  SRR4302542.4749103/1‑94 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGT                                                                                 <  SRR4302542.395897/94‑1 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGT                                                                                 >  SRR4302542.2998106/1‑94 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGT                                                                                 >  SRR4302542.4181088/1‑94 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGT                                                                                 >  SRR4302542.6918667/1‑94 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGG                                                                                 >  SRR4302542.2444561/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTT                                                                                <  SRR4302542.3933202/94‑1 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTT                                                                                <  SRR4302542.5788352/94‑1 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTT                                                                                <  SRR4302542.6901962/94‑1 (MQ=60)
                AATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCA                                                                              <  SRR4302542.402290/94‑1 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGA                                                                           <  SRR4302542.1756636/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGAT                                                                          <  SRR4302542.5757108/94‑1 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATG                                                                         >  SRR4302542.1162447/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCG                                                                       >  SRR4302542.6052998/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCG                                                                       <  SRR4302542.1304112/94‑1 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGAACGGGAACCAGTTCCAGATGCGT                                                                      >  SRR4302542.6036912/1‑94 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGTACCAGTTCCAGATGCGTC                                                                     >  SRR4302542.4079958/1‑94 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTC                                                                     >  SRR4302542.3240716/1‑94 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAT                                                                   <  SRR4302542.253258/94‑1 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAT                                                                   >  SRR4302542.709282/1‑94 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAT                                                                   >  SRR4302542.7333575/1‑94 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATT                                                                  >  SRR4302542.3914363/1‑94 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATT                                                                  <  SRR4302542.5066528/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATT                                                                  >  SRR4302542.7537466/1‑94 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTG                                                                 <  SRR4302542.654623/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTG                                                                 >  SRR4302542.3466192/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGGACCAGTTCCAGATGCGTCATTGCG                                                               >  SRR4302542.5923656/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCG                                                               >  SRR4302542.2198322/1‑94 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGC                                                             >  SRR4302542.6953102/1‑94 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGC                                                             >  SRR4302542.2277232/1‑94 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCCGC                                                             >  SRR4302542.4708321/1‑94 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                            <  SRR4302542.122503/94‑1 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                            >  SRR4302542.2515411/1‑94 (MQ=60)
                                   TTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAA                                                           >  SRR4302542.144554/1‑94 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCT                                                        >  SRR4302542.3809544/1‑94 (MQ=60)
                                       GCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTC                                                       >  SRR4302542.1538690/1‑94 (MQ=60)
                                        CGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCA                                                      >  SRR4302542.358320/1‑94 (MQ=60)
                                        CGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCA                                                      <  SRR4302542.863479/94‑1 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                     <  SRR4302542.7397243/94‑1 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                    >  SRR4302542.6539995/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                    >  SRR4302542.5703361/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                    <  SRR4302542.5971219/94‑1 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                    >  SRR4302542.2930932/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                    >  SRR4302542.1179671/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                   >  SRR4302542.4956843/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                   >  SRR4302542.7022227/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                   >  SRR4302542.1373376/1‑94 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC                                                  <  SRR4302542.1874885/94‑1 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC                                                  >  SRR4302542.687913/1‑94 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACG                                                 <  SRR4302542.5645928/94‑1 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACG                                                 >  SRR4302542.2507139/1‑94 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                >  SRR4302542.2321949/1‑94 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                >  SRR4302542.4606852/1‑94 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                >  SRR4302542.5555686/1‑94 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                >  SRR4302542.3587205/1‑94 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                               >  SRR4302542.3231276/1‑94 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                               <  SRR4302542.7251010/94‑1 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                               <  SRR4302542.2880537/94‑1 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                               <  SRR4302542.3297398/94‑1 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                               >  SRR4302542.992486/1‑94 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                              <  SRR4302542.6914218/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                              >  SRR4302542.7237033/1‑94 (MQ=60)
                                                 TTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGT                                             >  SRR4302542.5676504/1‑94 (MQ=60)
                                                 TTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGT                                             <  SRR4302542.3704238/94‑1 (MQ=60)
                                                 TTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGG                                             >  SRR4302542.5169501/1‑94 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                            <  SRR4302542.962306/94‑1 (MQ=60)
                                                   TTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCG                                           >  SRR4302542.2314786/1‑94 (MQ=60)
                                                   TTCCAAAACGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCG                                           <  SRR4302542.3561867/94‑1 (MQ=60)
                                                    TCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGT                                          >  SRR4302542.7201874/1‑94 (MQ=60)
                                                    TCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGT                                          >  SRR4302542.7307660/1‑94 (MQ=60)
                                                     CCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTC                                         >  SRR4302542.7102056/1‑94 (MQ=60)
                                                     CCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTC                                         >  SRR4302542.4124092/1‑94 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                        >  SRR4302542.3136926/1‑94 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                        <  SRR4302542.508532/94‑1 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                        <  SRR4302542.6120492/94‑1 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGGCGTCA                                        >  SRR4302542.1267788/1‑94 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                       >  SRR4302542.347609/1‑94 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                       <  SRR4302542.2637502/94‑1 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                       <  SRR4302542.2880069/94‑1 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                       <  SRR4302542.3370033/94‑1 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                      >  SRR4302542.6684424/1‑94 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                      <  SRR4302542.1453585/94‑1 (MQ=60)
                                                          ATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCC                                    <  SRR4302542.3982817/94‑1 (MQ=60)
                                                          ATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCC                                    <  SRR4302542.5708282/94‑1 (MQ=60)
                                                          ATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCC                                    <  SRR4302542.7389826/94‑1 (MQ=60)
                                                           TGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCA                                   >  SRR4302542.5480094/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                 <  SRR4302542.3059898/94‑1 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                 >  SRR4302542.5989984/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                 >  SRR4302542.6568516/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                 <  SRR4302542.656732/94‑1 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                 <  SRR4302542.447700/94‑1 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                 >  SRR4302542.861649/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                 >  SRR4302542.4651180/1‑94 (MQ=60)
                                                               GGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGCCAGGGCACGGA                               >  SRR4302542.3632146/1‑94 (MQ=60)
                                                                GCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAA                              <  SRR4302542.333860/94‑1 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                             >  SRR4302542.3865928/1‑94 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                             <  SRR4302542.1859840/94‑1 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                             >  SRR4302542.5848225/1‑94 (MQ=60)
                                                                  TCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGT                            <  SRR4302542.3836403/94‑1 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                           >  SRR4302542.3518789/1‑94 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                           <  SRR4302542.4051324/94‑1 (MQ=60)
                                                                    ACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTC                          >  SRR4302542.7143278/1‑94 (MQ=60)
                                                                      GCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTT                        <  SRR4302542.3283809/94‑1 (MQ=60)
                                                                       CCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTC                       >  SRR4302542.783929/1‑94 (MQ=60)
                                                                        CCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCA                      >  SRR4302542.6530666/1‑94 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                     <  SRR4302542.1537/94‑1 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                     >  SRR4302542.1338415/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                    >  SRR4302542.6961076/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                    >  SRR4302542.2567817/1‑94 (MQ=60)
                                                                           CCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTC                   <  SRR4302542.4944064/94‑1 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCTGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                  >  SRR4302542.2217439/1‑94 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                  >  SRR4302542.714553/1‑94 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                  <  SRR4302542.3443892/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                 >  SRR4302542.3734623/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                 >  SRR4302542.1369239/1‑94 (MQ=60)
                                                                                gcgGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG              <  SRR4302542.3747970/91‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG              <  SRR4302542.3500287/94‑1 (MQ=60)
                                                                                 GCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGC             <  SRR4302542.6676492/94‑1 (MQ=60)
                                                                                   GCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAC           >  SRR4302542.6484075/1‑94 (MQ=60)
                                                                                   GCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGCCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAC           >  SRR4302542.3506828/1‑94 (MQ=60)
                                                                                    CGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCGCGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACC          <  SRR4302542.5933792/94‑1 (MQ=60)
                                                                                    CGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACC          <  SRR4302542.882802/94‑1 (MQ=60)
                                                                                    CGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACC          >  SRR4302542.4814631/1‑94 (MQ=60)
                                                                                     GACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC         <  SRR4302542.1110012/94‑1 (MQ=60)
                                                                                          gcgGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCT    <  SRR4302542.6595597/91‑1 (MQ=60)
                                                                                          gcgGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCT    <  SRR4302542.553610/91‑1 (MQ=60)
                                                                                          gcgGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCT    >  SRR4302542.972763/4‑94 (MQ=60)
                                                                                           cgGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTA   <  SRR4302542.1584978/92‑1 (MQ=60)
                                                                                           G‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC  <  SRR4302542.7464600/94‑1 (MQ=60)
                                                                                           G‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC  >  SRR4302542.4063243/1‑94 (MQ=60)
                                                                                           G‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC  <  SRR4302542.2690153/94‑1 (MQ=60)
                                                                                           G‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC  >  SRR4302542.1085585/1‑94 (MQ=60)
                                                                                              |                                                                                            
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC  >  NC_000913/3627049‑3627233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑