Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,754,634 T→C N274S (AAC→AGC)  yiaY ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,754,6340TC87.4% 718.7 / 75.9 286N274S (AAC→AGC) yiaYputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (22/14);  new base C (119/130);  total (141/145)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 289 total aligned reads displayed.

GGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAA  >  NC_000913/3754543‑3754723
                                                                                           |                                                                                         
ggCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGtt                                                                                          >  1:1065893/1‑93 (MQ=255)
 gCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTg                                                                                         >  1:4809434/1‑93 (MQ=255)
   acaGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTa                                                                                       >  1:6639146/1‑93 (MQ=255)
   acaGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTa                                                                                       <  2:4840615/93‑1 (MQ=255)
     aGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGa                                                                                     >  2:3213317/1‑93 (MQ=255)
     aGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGa                                                                                     >  1:6589839/1‑93 (MQ=255)
      gTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGaa                                                                                    >  2:4392206/1‑93 (MQ=255)
      gTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGaa                                                                                    >  2:1478173/1‑93 (MQ=255)
       tCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATGCCTGAACGTGCGGCAGCAAAACGGCGTTACATTCACCGTGTGGCAGGCTGTAGaaa                                                                                   <  2:2192201/93‑1 (MQ=255)
       tCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGaaa                                                                                   <  1:3523960/93‑1 (MQ=255)
       tCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGaaa                                                                                   <  2:5743790/93‑1 (MQ=255)
       tCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGaaa                                                                                   <  2:7211505/93‑1 (MQ=255)
        cACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAAc                                                                                  <  1:5191370/93‑1 (MQ=255)
        cACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAAc                                                                                  <  1:1110628/93‑1 (MQ=255)
          cGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCg                                                                                <  1:3636809/93‑1 (MQ=255)
            cAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGcc                                                                              >  1:3282073/1‑93 (MQ=255)
             aGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGccc                                                                             >  1:1463751/1‑93 (MQ=255)
             aGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGccc                                                                             <  2:6619305/93‑1 (MQ=255)
             aGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGccc                                                                             <  1:549742/93‑1 (MQ=255)
              gACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCa                                                                            <  1:4340437/93‑1 (MQ=255)
              gACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCa                                                                            <  2:4137435/93‑1 (MQ=255)
               aCGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAg                                                                           >  1:6720817/1‑93 (MQ=255)
                cGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGc                                                                          <  1:2312682/93‑1 (MQ=255)
                  tGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTg                                                                        >  1:5095325/1‑93 (MQ=255)
                   gCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTgg                                                                       <  1:4554030/93‑1 (MQ=255)
                   gCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTgg                                                                       <  1:2495604/93‑1 (MQ=255)
                    cAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGt                                                                      >  2:469093/1‑93 (MQ=255)
                      gcggcgACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTgc                                                                    <  2:264609/93‑1 (MQ=255)
                      gcggcgACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTgc                                                                    >  1:1811362/1‑93 (MQ=255)
                       cggcgACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTgcg                                                                   >  2:4323651/1‑93 (MQ=255)
                       cggcgACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTgcg                                                                   >  2:3156736/1‑93 (MQ=255)
                        ggcgACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTgcgc                                                                  <  1:4241791/93‑1 (MQ=255)
                           gACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCAt                                                               >  1:4988642/1‑93 (MQ=255)
                           gACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCAt                                                               <  2:3492366/93‑1 (MQ=255)
                            aCTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATc                                                              >  2:2968200/1‑93 (MQ=255)
                            aCTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATc                                                              >  2:2074490/1‑93 (MQ=255)
                            aCTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATc                                                              <  1:1706029/93‑1 (MQ=255)
                             cTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCg                                                             >  2:1694803/1‑93 (MQ=255)
                              tttGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGc                                                            >  1:6877977/1‑93 (MQ=255)
                               ttGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCa                                                           >  2:727247/1‑93 (MQ=255)
                                tGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCAt                                                          <  1:6194079/93‑1 (MQ=255)
                                   tGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGaa                                                       <  2:4501889/93‑1 (MQ=255)
                                   tGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGaa                                                       <  2:1815080/93‑1 (MQ=255)
                                   tGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGaa                                                       >  1:4725154/1‑93 (MQ=255)
                                    gTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAAc                                                      <  2:332777/93‑1 (MQ=255)
                                    gTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAAc                                                      <  2:2310944/93‑1 (MQ=255)
                                     ttGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACa                                                     <  1:7451422/93‑1 (MQ=255)
                                     ttGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACa                                                     <  1:6523953/93‑1 (MQ=255)
                                       gAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATa                                                   >  2:6059191/1‑93 (MQ=255)
                                          tACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAAcc                                                >  1:1955497/1‑93 (MQ=255)
                                          tACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAAcc                                                >  2:4466902/1‑93 (MQ=255)
                                           aCCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAAccc                                               >  2:36852/1‑93 (MQ=255)
                                            ccTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACTTAACCCa                                              >  1:4824985/1‑93 (MQ=255)
                                            ccTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCa                                              >  2:6693741/1‑93 (MQ=255)
                                             cTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCag                                             >  2:3891922/1‑93 (MQ=255)
                                             cTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCag                                             >  2:2477992/1‑93 (MQ=255)
                                              tGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGTGCCATCGCATGAACATAACCCaga                                            >  2:4788113/1‑93 (MQ=255)
                                              tGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCaga                                            >  1:1002349/1‑93 (MQ=255)
                                              nnaaCGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCaga                                            <  1:2221570/91‑1 (MQ=255)
                                                 aCGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGaa                                         >  2:6168990/1‑93 (MQ=255)
                                                   gTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGc                                       >  1:990890/1‑93 (MQ=255)
                                                    tGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCa                                      <  1:5915315/93‑1 (MQ=255)
                                                    tGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCa                                      <  2:6316649/93‑1 (MQ=255)
                                                     ccGGCAGCAATACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCCGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCat                                     <  1:6221590/92‑1 (MQ=255)
                                                      cGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCatt                                    >  2:1358023/1‑93 (MQ=255)
                                                        gcagcaAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCattat                                  <  1:5246131/93‑1 (MQ=255)
                                                        gcagcaAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCattat                                  <  2:5516265/93‑1 (MQ=255)
                                                        gcagcaAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCattat                                  >  1:4923167/1‑93 (MQ=255)
                                                           gcaAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGa                               <  1:6391405/93‑1 (MQ=255)
                                                           gcaAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGa                               <  1:1366874/93‑1 (MQ=255)
                                                           gcaAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGa                               <  2:2821400/93‑1 (MQ=255)
                                                            caAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGaa                              <  1:7195853/93‑1 (MQ=255)
                                                            caAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGaa                              <  1:475096/93‑1 (MQ=255)
                                                                aCGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGcc                          <  2:1971732/93‑1 (MQ=255)
                                                                 cGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCa                         <  2:5085030/93‑1 (MQ=255)
                                                                 cGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCa                         >  2:792450/1‑93 (MQ=255)
                                                                 cGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCa                         >  1:7226211/1‑93 (MQ=255)
                                                                  ggCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCAt                        >  1:5667713/1‑93 (MQ=255)
                                                                   gCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATa                       >  1:5963299/1‑93 (MQ=255)
                                                                    cGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATAc                      <  2:1162960/93‑1 (MQ=255)
                                                                     gTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATAcc                     >  1:3385426/1‑93 (MQ=255)
                                                                     gTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATAcc                     <  2:5337522/93‑1 (MQ=255)
                                                                         catacaCCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGca                 >  2:5915587/1‑92 (MQ=255)
                                                                          atacaCCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGcaa                <  2:7151564/93‑3 (MQ=255)
                                                                           tacaCCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAg               <  2:6866458/93‑1 (MQ=255)
                                                                           tacaCCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAg               <  1:4149941/93‑1 (MQ=255)
                                                                           tacaCCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAg               >  2:1914075/1‑93 (MQ=255)
                                                                             cacCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGa             >  1:5066121/1‑93 (MQ=255)
                                                                              acCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGaa            <  2:406222/93‑1 (MQ=255)
                                                                               ccGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAAc           >  2:7428852/1‑93 (MQ=255)
                                                                                cGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACt          <  1:5371816/93‑1 (MQ=255)
                                                                                cGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACt          >  1:878875/1‑93 (MQ=255)
                                                                                cGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACt          <  1:2904104/93‑1 (MQ=255)
                                                                                 gtgtGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTg         >  2:5141190/1‑93 (MQ=255)
                                                                                 gtgtGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTg         <  2:2760009/93‑1 (MQ=255)
                                                                                 gtgtGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTg         >  2:3028500/1‑93 (MQ=255)
                                                                                  tgtgGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTgg        >  2:3931502/1‑93 (MQ=255)
                                                                                      gCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCAt    >  1:3938249/1‑93 (MQ=255)
                                                                                      gCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCAt    <  2:4218337/93‑1 (MQ=255)
                                                                                        aGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCATaa  >  1:7067225/1‑93 (MQ=255)
                                                                                           |                                                                                         
GGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAA  >  NC_000913/3754543‑3754723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCA  >  NC_000913/3754541‑3754727
                                                                                             |                                                                                             
GCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGC                                                                                               >  SRR4302542.829356/1‑94 (MQ=60)
 CGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTT                                                                                              >  SRR4302542.1075052/1‑94 (MQ=60)
 CGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCT                                                                                              >  SRR4302542.7492551/1‑94 (MQ=60)
 CGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCT                                                                                              >  SRR4302542.1600286/1‑94 (MQ=60)
  GGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTG                                                                                             >  SRR4302542.4856285/1‑94 (MQ=60)
  GGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTG                                                                                             <  SRR4302542.2334061/94‑1 (MQ=60)
  GGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTCCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTG                                                                                             <  SRR4302542.6271761/94‑1 (MQ=60)
    CACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTA                                                                                           >  SRR4302542.6704584/1‑94 (MQ=60)
    CACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTA                                                                                           >  SRR4302542.7180467/1‑94 (MQ=60)
    CACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTA                                                                                           <  SRR4302542.5047043/94‑1 (MQ=60)
    CACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTA                                                                                           >  SRR4302542.5124589/1‑94 (MQ=60)
    CACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTA                                                                                           >  SRR4302542.1815145/1‑94 (MQ=60)
     ACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAG                                                                                          >  SRR4302542.5709841/1‑94 (MQ=60)
      CAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGA                                                                                         >  SRR4302542.6654774/1‑94 (MQ=60)
       AGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAA                                                                                        <  SRR4302542.3402314/94‑1 (MQ=60)
         TCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAAC                                                                                      <  SRR4302542.3556888/94‑1 (MQ=60)
         TCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAAC                                                                                      >  SRR4302542.3332676/1‑94 (MQ=60)
          CACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACC                                                                                     <  SRR4302542.5241804/94‑1 (MQ=60)
          CACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACC                                                                                     >  SRR4302542.4817646/1‑94 (MQ=60)
          CACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACC                                                                                     <  SRR4302542.1120131/94‑1 (MQ=60)
           ACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCG                                                                                    <  SRR4302542.5957558/94‑1 (MQ=60)
           ACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCG                                                                                    >  SRR4302542.4894162/1‑94 (MQ=60)
            CGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGC                                                                                   >  SRR4302542.4864940/1‑94 (MQ=60)
            CGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGC                                                                                   <  SRR4302542.3670721/94‑1 (MQ=60)
             GCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCC                                                                                  >  SRR4302542.3313035/1‑94 (MQ=60)
              CAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCC                                                                                 >  SRR4302542.1476388/1‑94 (MQ=60)
               AGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCA                                                                                <  SRR4302542.554486/94‑1 (MQ=60)
                GACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAG                                                                               >  SRR4302542.931076/1‑94 (MQ=60)
                GACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAG                                                                               <  SRR4302542.2213548/94‑1 (MQ=60)
                GACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAG                                                                               >  SRR4302542.6787006/1‑94 (MQ=60)
                GACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAG                                                                               <  SRR4302542.4383086/94‑1 (MQ=60)
                  CGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCT                                                                             <  SRR4302542.2332535/94‑1 (MQ=60)
                   GTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTG                                                                            >  SRR4302542.5144837/1‑94 (MQ=60)
                    TGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGG                                                                           <  SRR4302542.1876374/94‑1 (MQ=60)
                     GCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGT                                                                          <  SRR4302542.2519808/94‑1 (MQ=60)
                     GCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGT                                                                          <  SRR4302542.3029091/94‑1 (MQ=60)
                     GCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGT                                                                          <  SRR4302542.4598592/94‑1 (MQ=60)
                       AGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGC                                                                        >  SRR4302542.1827059/1‑94 (MQ=60)
                        GCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCG                                                                       <  SRR4302542.3445378/94‑1 (MQ=60)
                         CGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGC                                                                      >  SRR4302542.7159335/1‑94 (MQ=60)
                         CGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGC                                                                      >  SRR4302542.788104/1‑94 (MQ=60)
                         CGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGC                                                                      >  SRR4302542.4750861/1‑94 (MQ=60)
                         CGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGC                                                                      >  SRR4302542.4532202/1‑94 (MQ=60)
                          GGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCC                                                                     <  SRR4302542.4283597/94‑1 (MQ=60)
                          GGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCC                                                                     >  SRR4302542.4864394/1‑94 (MQ=60)
                            CGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCAT                                                                   >  SRR4302542.265192/1‑94 (MQ=60)
                            CGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCAT                                                                   >  SRR4302542.4772489/1‑94 (MQ=60)
                            CGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCAT                                                                   >  SRR4302542.5037056/1‑94 (MQ=60)
                              ACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCG                                                                 <  SRR4302542.1720815/94‑1 (MQ=60)
                               CTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGC                                                                >  SRR4302542.6945537/1‑94 (MQ=60)
                               CTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGC                                                                >  SRR4302542.1410416/1‑94 (MQ=60)
                                  TGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATG                                                             <  SRR4302542.6254412/94‑1 (MQ=60)
                                   GCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGA                                                            >  SRR4302542.5722395/1‑94 (MQ=60)
                                   GCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGA                                                            <  SRR4302542.4982010/94‑1 (MQ=60)
                                    CTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAA                                                           <  SRR4302542.6013136/94‑1 (MQ=60)
                                    CTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAA                                                           >  SRR4302542.4771205/1‑94 (MQ=60)
                                     TGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAAC                                                          >  SRR4302542.7133186/1‑94 (MQ=60)
                                     TGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAAC                                                          >  SRR4302542.904660/1‑94 (MQ=60)
                                     TGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAAC                                                          <  SRR4302542.7521695/94‑1 (MQ=60)
                                       TTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACAT                                                        <  SRR4302542.6588240/94‑1 (MQ=60)
                                       TTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACAT                                                        <  SRR4302542.7524384/94‑1 (MQ=60)
                                        TGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACCCCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATA                                                       >  SRR4302542.7273497/1‑94 (MQ=60)
                                        TGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATA                                                       <  SRR4302542.109163/94‑1 (MQ=60)
                                         GAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAA                                                      <  SRR4302542.1859148/94‑1 (MQ=60)
                                          AATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAAC                                                     <  SRR4302542.667624/94‑1 (MQ=60)
                                           ATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACC                                                    >  SRR4302542.1972409/1‑94 (MQ=60)
                                            TACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCC                                                   <  SRR4302542.5460959/94‑1 (MQ=60)
                                             ACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACTTAACCCA                                                  >  SRR4302542.4872009/1‑94 (MQ=60)
                                              CCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAG                                                 >  SRR4302542.1108391/1‑94 (MQ=60)
                                               CTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGA                                                >  SRR4302542.1066152/1‑94 (MQ=60)
                                               CTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGA                                                >  SRR4302542.5534219/1‑94 (MQ=60)
                                               CTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGA                                                >  SRR4302542.6687209/1‑94 (MQ=60)
                                               CTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGA                                                >  SRR4302542.691241/1‑94 (MQ=60)
                                               CTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGA                                                >  SRR4302542.1010976/1‑94 (MQ=60)
                                                TGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAG                                               <  SRR4302542.6336271/94‑1 (MQ=60)
                                                TGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAG                                               <  SRR4302542.7488509/94‑1 (MQ=60)
                                                TGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAG                                               <  SRR4302542.137588/94‑1 (MQ=60)
                                                NNAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAG                                               <  SRR4302542.2240680/94‑1 (MQ=60)
                                                   ACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAG                                            <  SRR4302542.4780297/94‑1 (MQ=60)
                                                    CGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGC                                           >  SRR4302542.999421/1‑94 (MQ=60)
                                                    CGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGC                                           >  SRR4302542.59639/1‑94 (MQ=60)
                                                    CGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGC                                           >  SRR4302542.1798119/1‑94 (MQ=60)
                                                     GTGCGGCAGGAAAACGGAGTTACATAAACCGTGTGACAGGCTGTAGAAACCAGCCAGCTGGTGCGCCATCGCATGAACATAACGCAAAGAAGCA                                          >  SRR4302542.363855/1‑94 (MQ=53)
                                                      TGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCAT                                         <  SRR4302542.5071569/94‑1 (MQ=60)
                                                      TGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCAT                                         <  SRR4302542.5972637/94‑1 (MQ=60)
                                                      TGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCAT                                         <  SRR4302542.1590734/94‑1 (MQ=60)
                                                       GCGGCAGCAAAACGGCGTTACATACACCGGGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGGGCCATCGCATGAACATAACCCAGAGAAGCATT                                        >  SRR4302542.6650726/1‑94 (MQ=60)
                                                       CCGGCAGCAATACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCCGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATT                                        <  SRR4302542.6282861/94‑1 (MQ=60)
                                                        CGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTA                                       <  SRR4302542.875034/94‑1 (MQ=60)
                                                        CGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTA                                       >  SRR4302542.3753371/1‑94 (MQ=60)
                                                         GGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTAT                                      <  SRR4302542.3618033/94‑1 (MQ=60)
                                                         GGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTAT                                      >  SRR4302542.4971026/1‑94 (MQ=60)
                                                          GCAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATT                                     <  SRR4302542.5297124/94‑1 (MQ=60)
                                                           CAGCAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTG                                    <  SRR4302542.2268209/94‑1 (MQ=60)
                                                             GCAAAACGGCGTTACATGCACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAA                                  <  SRR4302542.4729708/94‑1 (MQ=60)
                                                             GCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAA                                  <  SRR4302542.6727499/94‑1 (MQ=60)
                                                             GCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAA                                  <  SRR4302542.6454370/94‑1 (MQ=60)
                                                             GCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAA                                  <  SRR4302542.1378689/94‑1 (MQ=60)
                                                              CAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAAC                                 <  SRR4302542.6879237/94‑1 (MQ=60)
                                                              CAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAAC                                 <  SRR4302542.479207/94‑1 (MQ=60)
                                                              CAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAAC                                 >  SRR4302542.5161676/1‑94 (MQ=60)
                                                              CAAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAAC                                 <  SRR4302542.7266473/94‑1 (MQ=60)
                                                               AAAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACG                                >  SRR4302542.4837011/1‑94 (MQ=60)
                                                                AAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGC                               >  SRR4302542.2283600/1‑94 (MQ=60)
                                                                AAACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGC                               >  SRR4302542.3798730/1‑94 (MQ=60)
                                                                 AACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCC                              >  SRR4302542.4198481/1‑94 (MQ=60)
                                                                 AACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCC                              <  SRR4302542.5338971/94‑1 (MQ=60)
                                                                  ACGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCA                             >  SRR4302542.7297096/1‑94 (MQ=60)
                                                                   CGGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCAT                            >  SRR4302542.5722763/1‑94 (MQ=60)
                                                                    GGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATA                           >  SRR4302542.6021030/1‑94 (MQ=60)
                                                                    GGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATA                           <  SRR4302542.4410764/94‑1 (MQ=60)
                                                                    GGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATA                           <  SRR4302542.488091/94‑1 (MQ=60)
                                                                    GGCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGAGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATA                           >  SRR4302542.2084518/1‑94 (MQ=60)
                                                                     GCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATAC                          <  SRR4302542.2859922/94‑1 (MQ=60)
                                                                     GCGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATAC                          <  SRR4302542.421257/94‑1 (MQ=60)
                                                                      CGTTACATACACCGTGTGGCAGGGTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACC                         <  SRR4302542.2501955/94‑1 (MQ=60)
                                                                      CGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACC                         <  SRR4302542.6237882/94‑1 (MQ=60)
                                                                      CGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACC                         >  SRR4302542.3417212/1‑94 (MQ=60)
                                                                      CGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACC                         <  SRR4302542.4313494/94‑1 (MQ=60)
                                                                      CGTTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACC                         >  SRR4302542.523692/1‑94 (MQ=60)
                                                                        TTACATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGG                       >  SRR4302542.673767/1‑94 (MQ=60)
                                                                           CATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAA                    >  SRR4302542.1459802/1‑94 (MQ=60)
                                                                            ATACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAG                   >  SRR4302542.3035375/1‑94 (MQ=60)
                                                                             TACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGG                  >  SRR4302542.1191434/1‑94 (MQ=60)
                                                                             TACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGG                  <  SRR4302542.4190814/94‑1 (MQ=60)
                                                                              ACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGA                 >  SRR4302542.5115354/1‑94 (MQ=60)
                                                                              ACACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGA                 <  SRR4302542.5202266/94‑1 (MQ=60)
                                                                               CACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAA                >  SRR4302542.6629354/1‑94 (MQ=60)
                                                                               CACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAA                >  SRR4302542.1631449/1‑94 (MQ=60)
                                                                                ACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAAC               <  SRR4302542.6525867/94‑1 (MQ=60)
                                                                                ACCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAAC               <  SRR4302542.6980001/94‑1 (MQ=60)
                                                                                 CCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACT              <  SRR4302542.5006450/94‑1 (MQ=60)
                                                                                 CCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACT              <  SRR4302542.6508665/94‑1 (MQ=60)
                                                                                 CCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACT              >  SRR4302542.886463/1‑94 (MQ=60)
                                                                                 CCGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACT              >  SRR4302542.4088892/1‑94 (MQ=60)
                                                                                  CGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTG             >  SRR4302542.3463865/1‑94 (MQ=60)
                                                                                  CGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTG             <  SRR4302542.5424019/94‑1 (MQ=60)
                                                                                  CGTGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTG             <  SRR4302542.2931975/94‑1 (MQ=60)
                                                                                   GTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGG            >  SRR4302542.5804517/1‑94 (MQ=60)
                                                                                    TGTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGG           <  SRR4302542.4158225/94‑1 (MQ=60)
                                                                                     GTGGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGC          <  SRR4302542.6187524/94‑1 (MQ=60)
                                                                                      TGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCA         >  SRR4302542.5263462/1‑94 (MQ=60)
                                                                                       GGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCAT        >  SRR4302542.6325750/1‑94 (MQ=60)
                                                                                       GGCAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCAT        >  SRR4302542.3977150/1‑94 (MQ=60)
                                                                                         CAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCATAA      >  SRR4302542.5335916/1‑94 (MQ=60)
                                                                                         CAGGCTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCATAA      >  SRR4302542.7136591/1‑94 (MQ=60)
                                                                                             CTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCATAAGCCA  >  SRR4302542.3240078/1‑94 (MQ=60)
                                                                                             CTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCAAGGAACTGGGCATAAGCCA  >  SRR4302542.3890929/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGGTTGTAGAAACCGCCCAGCTGGTGCGCCATCGCATGAACATAACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCA  >  NC_000913/3754541‑3754727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑