Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,823,984 A→G Q383R (CAG→CGG)  recG → DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,823,9840AG84.9% 767.8 / 132.4 332Q383R (CAG→CGG) recGDNA helicase
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base G (119/163);  total (142/190)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

Only 100 of 336 total aligned reads displayed.

GGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGA  >  NC_000913/3823893‑3824073
                                                                                           |                                                                                         
 gTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCggg                                                                                         >  2:2881603/1‑93 (MQ=255)
 gTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCggg                                                                                         >  2:4423579/1‑93 (MQ=255)
  tAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGa                                                                                        <  1:4813531/93‑1 (MQ=255)
    aGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAAc                                                                                      <  1:5850783/93‑1 (MQ=255)
     gCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACa                                                                                     >  1:3497871/1‑93 (MQ=255)
     gCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACa                                                                                     <  2:6582083/93‑1 (MQ=255)
     gCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACa                                                                                     <  2:5588728/93‑1 (MQ=255)
     gCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACa                                                                                     >  2:4141699/1‑93 (MQ=255)
      cAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAg                                                                                    <  2:6847205/93‑1 (MQ=255)
       aGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAgg                                                                                   <  1:1544494/93‑1 (MQ=255)
       aGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAgg                                                                                   <  2:3670255/93‑1 (MQ=255)
        gAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGt                                                                                  >  1:4519788/1‑93 (MQ=255)
          aaGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGc                                                                                >  1:2395293/1‑93 (MQ=255)
            ggTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAg                                                                              <  2:5087319/93‑1 (MQ=255)
            ggTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAg                                                                              <  1:3260595/93‑1 (MQ=255)
             gTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGt                                                                             <  1:6030492/93‑1 (MQ=255)
             gTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGt                                                                             >  2:2172985/1‑93 (MQ=255)
              tAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGCGCAGtt                                                                            >  1:3209688/1‑93 (MQ=255)
                 aGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTaa                                                                         <  1:4607666/93‑1 (MQ=255)
                  gCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAc                                                                        <  2:5820844/93‑1 (MQ=255)
                  gCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAc                                                                        >  1:980360/1‑93 (MQ=255)
                   cACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACg                                                                       <  2:3294927/93‑1 (MQ=255)
                   cACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACg                                                                       <  2:270990/93‑1 (MQ=255)
                    aCGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACgg                                                                      <  2:1436138/93‑1 (MQ=255)
                    aCGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACgg                                                                      <  2:5122195/93‑1 (MQ=255)
                    aCGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACgg                                                                      <  1:2127281/93‑1 (MQ=255)
                      ggCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGcc                                                                    <  1:6669428/93‑1 (MQ=255)
                      ggCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGcc                                                                    >  2:4791717/1‑93 (MQ=255)
                       gCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACGGGTGCAGTTTAACGGCCt                                                                   <  1:1075988/93‑1 (MQ=255)
                       gCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCt                                                                   <  1:2787537/93‑1 (MQ=255)
                       gCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCt                                                                   <  1:2677648/93‑1 (MQ=255)
                       gCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCt                                                                   >  1:3103101/1‑93 (MQ=255)
                         tGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTgg                                                                 <  2:5538472/93‑1 (MQ=255)
                         tGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTgg                                                                 >  2:1222907/1‑93 (MQ=255)
                         tGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTgg                                                                 >  2:266850/1‑93 (MQ=255)
                          ggCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGgc                                                                <  1:4423494/93‑1 (MQ=255)
                          ggCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGgc                                                                >  2:3193187/1‑93 (MQ=255)
                           gCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGgcg                                                               >  2:4875849/1‑93 (MQ=255)
                              cagcagGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTg                                                            <  1:7530112/93‑1 (MQ=255)
                               agcagGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTgg                                                           <  1:5623497/93‑1 (MQ=255)
                                  agGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGa                                                        >  1:5344468/1‑93 (MQ=255)
                                  agGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGa                                                        <  1:1026016/93‑1 (MQ=255)
                                  agGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGa                                                        <  1:7223608/93‑1 (MQ=255)
                                   ggAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACGGGTGCAGTTTAACGGCCTGGCGCTGGTGAt                                                       <  1:4709005/93‑1 (MQ=255)
                                   ggAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGAt                                                       <  2:7216744/93‑1 (MQ=255)
                                     aaGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCCGGCGCTGGTGATTa                                                     <  2:6449077/93‑1 (MQ=255)
                                     aaGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTa                                                     >  2:1564346/1‑93 (MQ=255)
                                      aGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTAt                                                    <  1:847888/93‑1 (MQ=255)
                                       gCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATc                                                   <  2:5727988/93‑1 (MQ=255)
                                         cATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcga                                                 <  2:2255688/93‑1 (MQ=255)
                                         cATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcga                                                 <  1:5259035/93‑1 (MQ=255)
                                          aTCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcgac                                                <  1:4042667/93‑1 (MQ=255)
                                          aTCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcgac                                                <  1:293833/93‑1 (MQ=255)
                                           tCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATcgacg                                               >  2:5495290/1‑93 (MQ=255)
                                             gCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGaa                                             <  2:7304680/93‑1 (MQ=255)
                                             gCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGaa                                             >  2:74890/1‑93 (MQ=255)
                                             gCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGaa                                             >  1:509229/1‑93 (MQ=255)
                                              ccAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAc                                            <  1:6474357/93‑1 (MQ=255)
                                              ccAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAc                                            <  2:3538723/93‑1 (MQ=255)
                                               cAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATCATCGACGAACa                                           <  1:1769082/93‑1 (MQ=255)
                                               cAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACa                                           <  2:7420937/93‑1 (MQ=255)
                                                 gCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGc                                         >  2:186900/1‑93 (MQ=255)
                                                   ggTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAt                                       <  2:1721101/93‑1 (MQ=255)
                                                    gTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATc                                      <  2:4015954/93‑1 (MQ=255)
                                                    gTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATc                                      <  1:30264/93‑1 (MQ=255)
                                                      cAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGt                                    <  2:3054009/93‑1 (MQ=255)
                                                      cAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGt                                    <  1:1695802/93‑1 (MQ=255)
                                                       aGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGtt                                   <  1:5970614/93‑1 (MQ=255)
                                                        ggTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGttt                                  >  2:5221898/1‑93 (MQ=255)
                                                        ggTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGttt                                  >  1:319070/1‑93 (MQ=255)
                                                         gTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGtttt                                 <  2:7060345/93‑1 (MQ=255)
                                                            cAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGc                              <  1:2339224/93‑1 (MQ=255)
                                                             aGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCg                             <  1:2079099/93‑1 (MQ=255)
                                                              gatgatTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGt                            >  1:1371452/1‑93 (MQ=255)
                                                              gatgatTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGt                            <  1:6594509/93‑1 (MQ=255)
                                                                 gatTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCa                         <  1:7085736/93‑1 (MQ=255)
                                                                 gatTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCa                         <  2:4927953/93‑1 (MQ=255)
                                                                   ttGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATc                       >  1:4380724/1‑93 (MQ=255)
                                                                   ttGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATc                       <  2:6719536/93‑1 (MQ=255)
                                                                    tGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCa                      <  2:3786321/93‑1 (MQ=255)
                                                                      tCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGc                    >  2:3094307/1‑93 (MQ=255)
                                                                       cGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGCGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCg                   <  1:6207892/93‑1 (MQ=255)
                                                                         gTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTc                 <  1:5758856/93‑1 (MQ=255)
                                                                         gTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTc                 <  2:2440064/93‑1 (MQ=255)
                                                                         gTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTc                 <  1:6864859/93‑1 (MQ=255)
                                                                         gTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATAGTTTTGGCGTGCATCAGCGTc                 >  1:2577979/1‑93 (MQ=255)
                                                                          tACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCt                <  2:4237349/93‑1 (MQ=255)
                                                                           acacacGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTg               <  1:3909999/93‑1 (MQ=255)
                                                                             acacGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGc             <  2:5975093/93‑1 (MQ=255)
                                                                              cacGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCa            >  2:4536903/1‑93 (MQ=255)
                                                                               acGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCAt           <  1:2467021/93‑1 (MQ=255)
                                                                                  ccATCTTGCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGGGATTATCGCCGAACAGCATCGTTTTGCCGTGCCTCAGAGTATCACATtgt        >  1:1967612/1‑93 (MQ=255)
                                                                                  ccATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATtgt        >  1:5395658/1‑93 (MQ=255)
                                                                                   cATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATtgtg       >  1:7327108/1‑93 (MQ=255)
                                                                                    aTCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTgg      >  1:5500530/1‑93 (MQ=255)
                                                                                    aTCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTgg      >  1:7442692/1‑93 (MQ=255)
                                                                                     tCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTggg     >  1:6326103/1‑93 (MQ=255)
                                                                                     tCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTggg     >  2:299650/1‑93 (MQ=255)
                                                                                       ttCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGgag   >  1:3360398/1‑93 (MQ=255)
                                                                                        tCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGgaga  >  2:6521389/1‑93 (MQ=255)
                                                                                           |                                                                                         
GGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGA  >  NC_000913/3823893‑3824073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGG  >  NC_000913/3823891‑3824077
                                                                                             |                                                                                             
CCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCG                                                                                               <  SRR4302542.4933189/94‑1 (MQ=60)
CCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCA                                                                                               <  SRR4302542.1496931/94‑1 (MQ=60)
 CGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGG                                                                                              >  SRR4302542.2692405/1‑94 (MQ=60)
 CGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGG                                                                                              >  SRR4302542.1531221/1‑94 (MQ=60)
 CGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGG                                                                                              <  SRR4302542.6088780/94‑1 (MQ=60)
 CGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAG                                                                                              >  SRR4302542.2355193/1‑94 (MQ=60)
  GGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGG                                                                                             <  SRR4302542.1682993/94‑1 (MQ=60)
  GGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGG                                                                                             <  SRR4302542.7568853/94‑1 (MQ=60)
   GTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGA                                                                                            <  SRR4302542.3267208/94‑1 (MQ=60)
   GTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGA                                                                                            >  SRR4302542.4876119/1‑94 (MQ=60)
    TAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAA                                                                                           >  SRR4302542.3467037/1‑94 (MQ=60)
    TAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAA                                                                                           <  SRR4302542.4860450/94‑1 (MQ=60)
      AGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACA                                                                                         <  SRR4302542.1897428/94‑1 (MQ=60)
      AGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACA                                                                                         >  SRR4302542.3530583/1‑94 (MQ=60)
      AGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACA                                                                                         <  SRR4302542.5907527/94‑1 (MQ=60)
       GCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGTTTGTCGGTACACACGCCATCTTCCGGGAACAG                                                                                        <  SRR4302542.4535671/94‑1 (MQ=60)
       GCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAG                                                                                        <  SRR4302542.5836395/94‑1 (MQ=60)
       GCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAG                                                                                        <  SRR4302542.280309/94‑1 (MQ=60)
       GCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAG                                                                                        <  SRR4302542.7483900/94‑1 (MQ=60)
       GCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAG                                                                                        <  SRR4302542.6669083/94‑1 (MQ=60)
       GCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCACCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAG                                                                                        <  SRR4302542.2035109/94‑1 (MQ=60)
        CAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGG                                                                                       <  SRR4302542.64288/94‑1 (MQ=60)
        CAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGG                                                                                       <  SRR4302542.2141552/94‑1 (MQ=60)
         AGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGT                                                                                      >  SRR4302542.375558/1‑94 (MQ=60)
         AGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGT                                                                                      <  SRR4302542.4574051/94‑1 (MQ=60)
         AGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGT                                                                                      >  SRR4302542.4564069/1‑94 (MQ=60)
         AGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGT                                                                                      <  SRR4302542.1557821/94‑1 (MQ=60)
           AANNNNANAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGC                                                                                    <  SRR4302542.6724846/94‑1 (MQ=60)
           AAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGC                                                                                    >  SRR4302542.2418326/1‑94 (MQ=60)
            AAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATAGTCGGTACACACGCCATCTTCCGGGAACAGGTGCA                                                                                   <  SRR4302542.6610013/94‑1 (MQ=60)
             AGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAG                                                                                  >  SRR4302542.308189/1‑94 (MQ=60)
             AGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAG                                                                                  <  SRR4302542.3349571/94‑1 (MQ=60)
             AGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAG                                                                                  <  SRR4302542.6021773/94‑1 (MQ=60)
              GGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGT                                                                                 <  SRR4302542.3291386/94‑1 (MQ=60)
               GTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTT                                                                                <  SRR4302542.5234656/94‑1 (MQ=60)
               GTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTT                                                                                <  SRR4302542.6088884/94‑1 (MQ=60)
               GTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGCGCAGTT                                                                                >  SRR4302542.3240077/1‑94 (MQ=60)
                TAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTT                                                                               >  SRR4302542.536083/1‑94 (MQ=60)
                 AAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTA                                                                              <  SRR4302542.3139206/94‑1 (MQ=60)
                  AAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAA                                                                             >  SRR4302542.6721109/1‑94 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            >  SRR4302542.988805/1‑94 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            <  SRR4302542.4062858/94‑1 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            <  SRR4302542.4426203/94‑1 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            <  SRR4302542.4652691/94‑1 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            <  SRR4302542.4868694/94‑1 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            >  SRR4302542.7020067/1‑94 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            <  SRR4302542.5801069/94‑1 (MQ=60)
                   AGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAAC                                                                            >  SRR4302542.2210599/1‑94 (MQ=60)
                    GCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACG                                                                           >  SRR4302542.716493/1‑94 (MQ=60)
                    GCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACG                                                                           <  SRR4302542.2507999/94‑1 (MQ=60)
                     CACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGG                                                                          >  SRR4302542.5889567/1‑94 (MQ=60)
                      ACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGC                                                                         <  SRR4302542.2145632/94‑1 (MQ=60)
                        GGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCT                                                                       <  SRR4302542.7437597/94‑1 (MQ=60)
                        GGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCT                                                                       <  SRR4302542.6735138/94‑1 (MQ=60)
                        GGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCT                                                                       <  SRR4302542.1164550/94‑1 (MQ=60)
                        GGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCT                                                                       <  SRR4302542.3499354/94‑1 (MQ=60)
                        GGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCT                                                                       <  SRR4302542.2122460/94‑1 (MQ=60)
                        GGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCT                                                                       >  SRR4302542.3132590/1‑94 (MQ=60)
                         GCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACGGGTGCAGTTTAACGGCCTG                                                                      <  SRR4302542.1085234/94‑1 (MQ=60)
                         GCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTG                                                                      <  SRR4302542.2703487/94‑1 (MQ=60)
                         GCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTG                                                                      <  SRR4302542.45993/94‑1 (MQ=60)
                         GCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTG                                                                      <  SRR4302542.4780170/94‑1 (MQ=60)
                         GCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTG                                                                      <  SRR4302542.2814378/94‑1 (MQ=60)
                         GCGGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTG                                                                      <  SRR4302542.6380606/94‑1 (MQ=60)
                          CTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGG                                                                     >  SRR4302542.1709880/1‑94 (MQ=60)
                          CTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGG                                                                     <  SRR4302542.2084098/94‑1 (MQ=60)
                           TGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGC                                                                    <  SRR4302542.3258342/94‑1 (MQ=60)
                           TGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGC                                                                    <  SRR4302542.3104839/94‑1 (MQ=60)
                            GGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCG                                                                   <  SRR4302542.5169472/94‑1 (MQ=60)
                            GGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCG                                                                   <  SRR4302542.4466937/94‑1 (MQ=60)
                              CACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCT                                                                 >  SRR4302542.2902005/1‑94 (MQ=60)
                                CAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGG                                                               <  SRR4302542.7604139/94‑1 (MQ=60)
                                 AGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGT                                                              >  SRR4302542.2975210/1‑94 (MQ=60)
                                 AGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGT                                                              <  SRR4302542.5678114/94‑1 (MQ=60)
                                  GCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTG                                                             <  SRR4302542.471912/94‑1 (MQ=60)
                                   CAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGA                                                            >  SRR4302542.2551280/1‑94 (MQ=60)
                                   CAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGA                                                            >  SRR4302542.1901528/1‑94 (MQ=60)
                                   CAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGA                                                            >  SRR4302542.5396387/1‑94 (MQ=60)
                                   CAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGA                                                            >  SRR4302542.5438454/1‑94 (MQ=60)
                                    AGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGAT                                                           <  SRR4302542.7294464/94‑1 (MQ=60)
                                    AGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGAT                                                           <  SRR4302542.1034858/94‑1 (MQ=60)
                                    AGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGAT                                                           <  SRR4302542.3787744/94‑1 (MQ=60)
                                     GGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACGGGTGCAGTTTAACGGCCTGGCGCTGGTGATT                                                          <  SRR4302542.4754937/94‑1 (MQ=60)
                                       AAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTAT                                                        >  SRR4302542.4413792/1‑94 (MQ=60)
                                        AGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATC                                                       >  SRR4302542.5603170/1‑94 (MQ=60)
                                        AGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATC                                                       <  SRR4302542.855196/94‑1 (MQ=60)
                                        AGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATC                                                       >  SRR4302542.1084703/1‑94 (MQ=60)
                                         GCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCATTTTAACGGCCTGGCGCTGGTGATTATCG                                                      <  SRR4302542.5700742/94‑1 (MQ=60)
                                         GCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCG                                                      >  SRR4302542.5533821/1‑94 (MQ=60)
                                         GCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCG                                                      <  SRR4302542.5752018/94‑1 (MQ=60)
                                          CCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGA                                                     <  SRR4302542.6313656/94‑1 (MQ=60)
                                          CCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGA                                                     >  SRR4302542.5924644/1‑94 (MQ=60)
                                           CATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGAC                                                    <  SRR4302542.5310141/94‑1 (MQ=60)
                                           CATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGAC                                                    <  SRR4302542.3245138/94‑1 (MQ=60)
                                            ATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                                                   >  SRR4302542.4445829/1‑94 (MQ=60)
                                            ATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                                                   >  SRR4302542.808563/1‑94 (MQ=60)
                                            ATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                                                   >  SRR4302542.6547588/1‑94 (MQ=60)
                                            ATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                                                   <  SRR4302542.4082556/94‑1 (MQ=60)
                                            ATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                                                   <  SRR4302542.7188872/94‑1 (MQ=60)
                                            ATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACG                                                   <  SRR4302542.296403/94‑1 (MQ=60)
                                             TCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGA                                                  >  SRR4302542.4289530/1‑94 (MQ=60)
                                              CGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAA                                                 <  SRR4302542.5555862/94‑1 (MQ=60)
                                              CGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAA                                                 >  SRR4302542.513649/1‑94 (MQ=60)
                                               GCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAAC                                                >  SRR4302542.3107812/1‑94 (MQ=60)
                                                CCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACA                                               <  SRR4302542.6079599/94‑1 (MQ=60)
                                                CCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACA                                               <  SRR4302542.6538143/94‑1 (MQ=60)
                                                CCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACA                                               <  SRR4302542.3163443/94‑1 (MQ=60)
                                                CCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACA                                               >  SRR4302542.1443067/1‑94 (MQ=60)
                                                 CAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAG                                              <  SRR4302542.456137/94‑1 (MQ=60)
                                                 CAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATCATCGACGAACAG                                              <  SRR4302542.1784419/94‑1 (MQ=60)
                                                 CAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAG                                              <  SRR4302542.977268/94‑1 (MQ=60)
                                                    CGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT                                           <  SRR4302542.1525581/94‑1 (MQ=60)
                                                     GGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATC                                          >  SRR4302542.2543432/1‑94 (MQ=60)
                                                     GGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATC                                          <  SRR4302542.2431717/94‑1 (MQ=60)
                                                     GGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATC                                          >  SRR4302542.5440958/1‑94 (MQ=60)
                                                      GTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCG                                         >  SRR4302542.5924211/1‑94 (MQ=60)
                                                      GTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCG                                         <  SRR4302542.30538/94‑1 (MQ=60)
                                                        CAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTT                                       <  SRR4302542.1710496/94‑1 (MQ=60)
                                                         AGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTT                                      <  SRR4302542.6028402/94‑1 (MQ=60)
                                                         AGGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTT                                      >  SRR4302542.2675805/1‑94 (MQ=60)
                                                         AGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTT                                      >  SRR4302542.321840/1‑94 (MQ=60)
                                                          GGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTT                                     >  SRR4302542.6072270/1‑94 (MQ=60)
                                                          GGTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTT                                     <  SRR4302542.247517/94‑1 (MQ=60)
                                                           GTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTG                                    >  SRR4302542.1865732/1‑94 (MQ=60)
                                                           GTGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACAGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTG                                    >  SRR4302542.7298595/1‑94 (MQ=60)
                                                            TGCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGG                                   <  SRR4302542.7178351/94‑1 (MQ=60)
                                                             GCAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGC                                  >  SRR4302542.4999005/1‑94 (MQ=60)
                                                              CAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCG                                 <  SRR4302542.2359262/94‑1 (MQ=60)
                                                              CAGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCG                                 >  SRR4302542.6789554/1‑94 (MQ=60)
                                                              CAGATGATTGTCGGTACACACGCCATCTTCCAGGAACCGGGGGAGTTTAACGGCCTGGCGCTGGTGATTATCGCCGAACcccgtcgctttaccg                                 >  SRR4302542.4074836/1‑79 (MQ=60)
                                                               AGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGT                                >  SRR4302542.1740952/1‑94 (MQ=60)
                                                               AGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGT                                <  SRR4302542.2097053/94‑1 (MQ=60)
                                                               AGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGT                                >  SRR4302542.1383307/1‑94 (MQ=60)
                                                               AGATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATGATCGACGAACAGCATCGTTTTGGCGT                                >  SRR4302542.7605568/1‑94 (MQ=60)
                                                                GATGATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTG                               <  SRR4302542.6659480/94‑1 (MQ=60)
                                                                  TGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCA                             >  SRR4302542.890328/1‑94 (MQ=60)
                                                                   GATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCAT                            <  SRR4302542.7155262/94‑1 (MQ=60)
                                                                   GATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCAT                            >  SRR4302542.3228666/1‑94 (MQ=60)
                                                                   GATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCAT                            <  SRR4302542.944201/94‑1 (MQ=60)
                                                                    ATTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATC                           >  SRR4302542.4423711/1‑94 (MQ=60)
                                                                     TTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCA                          >  SRR4302542.6428353/1‑94 (MQ=60)
                                                                     TTGTCGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCA                          >  SRR4302542.5329085/1‑94 (MQ=60)
                                                                     TTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCA                          <  SRR4302542.5446972/94‑1 (MQ=60)
                                                                     TTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCA                          <  SRR4302542.631973/94‑1 (MQ=60)
                                                                      TGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAG                         <  SRR4302542.1712371/94‑1 (MQ=60)
                                                                      TGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAG                         <  SRR4302542.6240162/94‑1 (MQ=60)
                                                                        TCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCG                       >  SRR4302542.6915083/1‑94 (MQ=60)
                                                                         CGGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGCGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGT                      <  SRR4302542.6269023/94‑1 (MQ=60)
                                                                         CGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGT                      <  SRR4302542.1073390/94‑1 (MQ=60)
                                                                          GGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTC                     >  SRR4302542.2614054/1‑94 (MQ=60)
                                                                          GGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTC                     <  SRR4302542.6169115/94‑1 (MQ=60)
                                                                          GGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTC                     >  SRR4302542.447154/1‑94 (MQ=60)
                                                                          GGTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATAGTTTTGGCGTGCATCAGCGTC                     >  SRR4302542.2602969/1‑94 (MQ=60)
                                                                           GTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCT                    <  SRR4302542.6932304/94‑1 (MQ=60)
                                                                           GTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCT                    >  SRR4302542.4759421/1‑94 (MQ=60)
                                                                           GTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCT                    <  SRR4302542.5814755/94‑1 (MQ=60)
                                                                           GTACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCT                    <  SRR4302542.7468148/94‑1 (MQ=60)
                                                                            TACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTG                   >  SRR4302542.7309933/1‑94 (MQ=60)
                                                                             ACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGG                  <  SRR4302542.3948585/94‑1 (MQ=60)
                                                                             ACACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGG                  <  SRR4302542.5487109/94‑1 (MQ=60)
                                                                             ACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGG                  >  SRR4302542.299092/1‑94 (MQ=60)
                                                                              CACACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGC                 >  SRR4302542.2402965/1‑94 (MQ=60)
                                                                                CACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCAT               <  SRR4302542.2934799/94‑1 (MQ=60)
                                                                                 ACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATT              <  SRR4302542.2490967/94‑1 (MQ=60)
                                                                                 ACGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATT              <  SRR4302542.4737970/94‑1 (MQ=60)
                                                                                 ACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATT              <  SRR4302542.1644027/94‑1 (MQ=60)
                                                                                  CGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTG             >  SRR4302542.7538100/1‑94 (MQ=60)
                                                                                  CGCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTG             <  SRR4302542.1285688/94‑1 (MQ=60)
                                                                                   GCCATCTTGCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGGGATTATCGCCGAACAGCATCGTTTTGCCGTGCctcagagtatcacattgt            >  SRR4302542.1984614/1‑76 (MQ=60)
                                                                                   GCCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGT            >  SRR4302542.2757410/1‑94 (MQ=60)
                                                                                   GCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGT            >  SRR4302542.3889453/1‑94 (MQ=60)
                                                                                   GCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGT            >  SRR4302542.5448063/1‑94 (MQ=60)
                                                                                    CCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTG           >  SRR4302542.2159397/1‑94 (MQ=60)
                                                                                    CCATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTG           >  SRR4302542.7398887/1‑94 (MQ=60)
                                                                                    CCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTG           >  SRR4302542.3236563/1‑94 (MQ=60)
                                                                                     CATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGG          <  SRR4302542.4179492/94‑1 (MQ=60)
                                                                                     CATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGG          >  SRR4302542.5553959/1‑94 (MQ=60)
                                                                                     CATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGG          >  SRR4302542.7515562/1‑94 (MQ=60)
                                                                                      ATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGG         >  SRR4302542.6388440/1‑94 (MQ=60)
                                                                                      ATCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCAACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGG         >  SRR4302542.3312374/1‑94 (MQ=60)
                                                                                       TCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGA        >  SRR4302542.7005683/1‑94 (MQ=60)
                                                                                       TCTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGA        <  SRR4302542.2712419/94‑1 (MQ=60)
                                                                                        CTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGatgcac       <  SRR4302542.6713316/94‑7 (MQ=60)
                                                                                        CTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAG       >  SRR4302542.3391982/1‑94 (MQ=60)
                                                                                        CTTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAG       >  SRR4302542.151136/1‑94 (MQ=60)
                                                                                         TTCCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGA      >  SRR4302542.4056257/1‑94 (MQ=60)
                                                                                           CCGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAA    <  SRR4302542.124968/94‑1 (MQ=60)
                                                                                            CGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAACG   <  SRR4302542.4919180/94‑1 (MQ=60)
                                                                                            CGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAG   <  SRR4302542.2656879/94‑1 (MQ=60)
                                                                                            CGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAG   >  SRR4302542.5027554/1‑94 (MQ=60)
                                                                                            CGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAG   <  SRR4302542.3273265/94‑1 (MQ=60)
                                                                                            CGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAG   >  SRR4302542.6192858/1‑94 (MQ=60)
                                                                                            CGGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAG   <  SRR4302542.6275301/94‑1 (MQ=60)
                                                                                             GGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGG  <  SRR4302542.6576141/94‑1 (MQ=60)
                                                                                             GGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGG  >  SRR4302542.4334280/1‑94 (MQ=60)
                                                                                             GGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGG  >  SRR4302542.4079176/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCGGTAAGCAGAAAGGTAAAGCACGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCATCGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGG  >  NC_000913/3823891‑3824077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑