Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,825,188 T→C M369V (ATG→GTG)  gltS ← glutamate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,825,1880TC83.9% 721.8 / 136.1 322M369V (ATG→GTG) gltSglutamate transport
Reads supporting (aligned to +/- strand):  ref base T (27/25);  new base C (154/116);  total (181/141)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 328 total aligned reads displayed.

AAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGC  >  NC_000913/3825096‑3825278
                                                                                            |                                                                                          
    aTCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGc                                                                                        <  1:5776107/93‑1 (MQ=255)
    aTCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGc                                                                                        <  1:6374463/93‑1 (MQ=255)
    aTCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGc                                                                                        <  1:4060276/93‑1 (MQ=255)
      cGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGa                                                                                      <  1:3432722/93‑1 (MQ=255)
       ggCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGAc                                                                                     >  1:2752450/1‑93 (MQ=255)
        gCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACg                                                                                    <  2:7218834/93‑1 (MQ=255)
         cAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACgg                                                                                   >  2:2960597/1‑93 (MQ=255)
            cATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGcc                                                                                >  1:7112559/1‑93 (MQ=255)
             aTCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCa                                                                               >  1:7197405/1‑93 (MQ=255)
             aTCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCa                                                                               >  2:5533283/1‑93 (MQ=255)
             aTCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCa                                                                               >  1:1616489/1‑93 (MQ=255)
             aTCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCa                                                                               >  2:5751696/1‑93 (MQ=255)
              tCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCaa                                                                              <  1:2696159/93‑1 (MQ=255)
              tCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCaa                                                                              >  2:2211490/1‑93 (MQ=255)
              tCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCaa                                                                              >  1:3662010/1‑93 (MQ=255)
               cAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCaaa                                                                             <  2:1085566/93‑1 (MQ=255)
               cAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCaaa                                                                             <  2:190331/93‑1 (MQ=255)
                aaaTACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAg                                                                            >  1:4737018/1‑93 (MQ=255)
                aaaTACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAg                                                                            >  2:275936/1‑93 (MQ=255)
                aaaTACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAg                                                                            <  2:5222339/93‑1 (MQ=255)
                 aaTACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGc                                                                           <  1:3540496/93‑1 (MQ=255)
                   tACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGt                                                                         <  2:2636499/93‑1 (MQ=255)
                   tACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGt                                                                         >  2:3382897/1‑93 (MQ=255)
                    aCAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGtt                                                                        <  2:4372264/93‑1 (MQ=255)
                      aaCTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCa                                                                      <  2:1504817/93‑1 (MQ=255)
                      aaCTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCa                                                                      <  2:6297434/93‑1 (MQ=255)
                       aCTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAg                                                                     >  1:4218780/1‑93 (MQ=255)
                        cTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGt                                                                    >  1:1405159/1‑93 (MQ=255)
                        cTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGt                                                                    >  2:7012451/1‑93 (MQ=255)
                         ttAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTg                                                                   >  2:4978668/1‑93 (MQ=255)
                         ttAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTg                                                                   <  1:3016120/93‑1 (MQ=255)
                             ttACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCg                                                               >  2:1296929/1‑93 (MQ=255)
                              tACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGc                                                              >  2:775452/1‑93 (MQ=255)
                                ccAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCt                                                            >  1:2281810/1‑93 (MQ=255)
                                  aGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGc                                                          >  1:3809683/1‑93 (MQ=255)
                                   gcgcATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCa                                                         >  2:6563466/1‑93 (MQ=255)
                                     gcATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATg                                                       <  2:6793861/93‑1 (MQ=255)
                                      cATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGt                                                      <  1:6690204/93‑1 (MQ=255)
                                      cATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGt                                                      <  1:5631684/93‑1 (MQ=255)
                                      cATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGt                                                      <  2:7156756/93‑1 (MQ=255)
                                      cATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGt                                                      <  1:923162/93‑1 (MQ=255)
                                       aTTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGtt                                                     <  2:4030957/93‑1 (MQ=255)
                                        ttGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTg                                                    <  2:6168476/93‑1 (MQ=255)
                                         tGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTgg                                                   <  1:2199651/93‑1 (MQ=255)
                                         tGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTgg                                                   <  1:2830115/93‑1 (MQ=255)
                                         tGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTgg                                                   <  2:7467516/93‑1 (MQ=255)
                                          gACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGc                                                  >  1:6050513/1‑93 (MQ=255)
                                          gACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGc                                                  >  2:6357686/1‑93 (MQ=255)
                                          gACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGc                                                  <  2:4573355/93‑1 (MQ=255)
                                          gACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGc                                                  >  2:3955437/1‑93 (MQ=255)
                                          gACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGc                                                  >  2:7259281/1‑93 (MQ=255)
                                           aCGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCg                                                 <  1:248997/93‑1 (MQ=255)
                                             gATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGAt                                               <  2:3807444/93‑1 (MQ=255)
                                              atatCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGAtt                                              <  1:1330131/93‑1 (MQ=255)
                                                  cGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCg                                          >  2:6253483/1‑93 (MQ=255)
                                                   gATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGt                                         >  2:3340493/1‑93 (MQ=255)
                                                   gATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGt                                         <  1:514880/93‑1 (MQ=255)
                                                    aTAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGtt                                        >  1:1086617/1‑93 (MQ=255)
                                                    aTAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGtt                                        <  1:6837035/93‑1 (MQ=255)
                                                     tAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTg                                       >  1:1867112/1‑93 (MQ=255)
                                                     tAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTg                                       >  2:4705768/1‑93 (MQ=255)
                                                     tAAACAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTg                                       >  2:7371773/1‑93 (MQ=255)
                                                       aaGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGc                                     >  1:5595596/1‑93 (MQ=255)
                                                       aaGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGc                                     >  1:5371852/1‑93 (MQ=255)
                                                        aGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCg                                    >  2:2125537/1‑93 (MQ=255)
                                                        aGAACGCACCGACCATCGGCACCACCAAAAACGCCACGCGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCg                                    <  1:6330944/93‑1 (MQ=255)
                                                         gAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGt                                   <  2:5999668/93‑1 (MQ=255)
                                                         gAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGt                                   >  2:7101415/1‑93 (MQ=255)
                                                         gAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGt                                   <  1:4325790/93‑1 (MQ=255)
                                                          aaCGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGtt                                  <  1:7329571/93‑1 (MQ=255)
                                                           aCGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTg                                 >  1:4475214/1‑93 (MQ=255)
                                                            cGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGc                                <  2:6916818/93‑1 (MQ=255)
                                                            cGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGc                                <  2:2444095/93‑1 (MQ=255)
                                                            cGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGc                                <  2:5494011/93‑1 (MQ=255)
                                                             gCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCa                               >  1:3257110/1‑93 (MQ=255)
                                                              cACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCAc                              >  1:7447743/1‑93 (MQ=255)
                                                                ccGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCg                            >  2:3227909/1‑93 (MQ=255)
                                                                ccGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCg                            >  2:1805714/1‑93 (MQ=255)
                                                                 cGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGa                           <  1:6467737/93‑1 (MQ=255)
                                                                  gACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTACACCCAg                          >  2:5611999/1‑93 (MQ=255)
                                                                    ccATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGc                        >  2:446302/1‑93 (MQ=255)
                                                                       tCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCaa                     <  2:2019383/93‑1 (MQ=255)
                                                                         ggCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCaaaa                   >  1:1286417/1‑93 (MQ=255)
                                                                            accaccAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACca                >  2:4939358/1‑93 (MQ=255)
                                                                              caccaAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACcaca              <  1:3040880/93‑1 (MQ=255)
                                                                               accaAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAg             >  1:5006535/1‑93 (MQ=255)
                                                                               accaAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAg             <  1:4189821/93‑1 (MQ=255)
                                                                               accaAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAg             <  2:4645274/93‑1 (MQ=255)
                                                                                 caAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTg           >  1:2132603/1‑93 (MQ=255)
                                                                                  aaaaaCGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGa          <  1:6917444/93‑1 (MQ=255)
                                                                                   aaaaCGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAc         >  2:3944448/1‑93 (MQ=255)
                                                                                   aaaaCGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAc         >  1:6569317/1‑93 (MQ=255)
                                                                                    aaaCGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAcc        >  2:4138434/1‑93 (MQ=255)
                                                                                     aaCGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAccc       <  2:1749705/93‑1 (MQ=255)
                                                                                       cGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGc     >  2:4524005/1‑93 (MQ=255)
                                                                                        gCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGca    >  1:2010372/1‑93 (MQ=255)
                                                                                        gCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGca    >  1:1743537/1‑93 (MQ=255)
                                                                                        gCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGca    <  2:501624/93‑1 (MQ=255)
                                                                                         ccACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGcag   >  2:3479844/1‑93 (MQ=255)
                                                                                         ccACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGcag   >  1:6030554/1‑93 (MQ=255)
                                                                                            |                                                                                          
AAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGC  >  NC_000913/3825096‑3825278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAG  >  NC_000913/3825095‑3825281
                                                                                             |                                                                                             
CAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCAC                                                                                               <  SRR4302542.1705473/94‑1 (MQ=60)
 AAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATG                                                                                              <  SRR4302542.5232552/94‑1 (MQ=60)
 AAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACG                                                                                              <  SRR4302542.4028394/94‑1 (MQ=60)
  AAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGT                                                                                             <  SRR4302542.3469500/94‑1 (MQ=60)
   AAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTG                                                                                            <  SRR4302542.2384137/94‑1 (MQ=60)
    AATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGC                                                                                           <  SRR4302542.7288049/94‑1 (MQ=60)
     ATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCG                                                                                          <  SRR4302542.5832180/94‑1 (MQ=60)
     ATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCG                                                                                          <  SRR4302542.4212239/94‑1 (MQ=60)
     ATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCG                                                                                          <  SRR4302542.4100314/94‑1 (MQ=60)
     ATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCG                                                                                          <  SRR4302542.6437229/94‑1 (MQ=60)
       CGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGAC                                                                                        <  SRR4302542.3464860/94‑1 (MQ=60)
       CGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGAC                                                                                        >  SRR4302542.2778984/1‑94 (MQ=60)
         GCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGG                                                                                      <  SRR4302542.7138510/94‑1 (MQ=60)
          CAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGG                                                                                     <  SRR4302542.3054095/94‑1 (MQ=60)
           AACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGC                                                                                    >  SRR4302542.4779719/1‑94 (MQ=60)
           AACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGC                                                                                    >  SRR4302542.5680222/1‑94 (MQ=60)
            ACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCC                                                                                   >  SRR4302542.7182374/1‑94 (MQ=60)
             CATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCA                                                                                  >  SRR4302542.7268033/1‑94 (MQ=60)
             CATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCA                                                                                  >  SRR4302542.1630453/1‑94 (MQ=60)
              ATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAA                                                                                 >  SRR4302542.2396042/1‑94 (MQ=60)
              ATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAA                                                                                 >  SRR4302542.1802558/1‑94 (MQ=60)
              ATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAA                                                                                 >  SRR4302542.3696602/1‑94 (MQ=60)
               TCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAA                                                                                <  SRR4302542.2722158/94‑1 (MQ=60)
                CAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAG                                                                               <  SRR4302542.3641976/94‑1 (MQ=60)
                CAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAG                                                                               <  SRR4302542.6173814/94‑1 (MQ=60)
                CAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAG                                                                               >  SRR4302542.4783213/1‑94 (MQ=60)
                CAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGCGCGACGGGCCAAAG                                                                               <  SRR4302542.3387848/94‑1 (MQ=60)
                CAAATACAACTTAATTACCAGCGCATTGACAATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAG                                                                               >  SRR4302542.1736688/1‑94 (MQ=60)
                 AAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGC                                                                              <  SRR4302542.5430524/94‑1 (MQ=60)
                  AATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCG                                                                             <  SRR4302542.3573575/94‑1 (MQ=60)
                  AATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCG                                                                             >  SRR4302542.5700342/1‑94 (MQ=60)
                  AATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCG                                                                             <  SRR4302542.6502331/94‑1 (MQ=60)
                   ATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGT                                                                            <  SRR4302542.3168060/94‑1 (MQ=60)
                    TACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTT                                                                           <  SRR4302542.376431/94‑1 (MQ=60)
                      CAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCA                                                                         <  SRR4302542.304195/94‑1 (MQ=60)
                      CAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCA                                                                         >  SRR4302542.566101/1‑94 (MQ=60)
                       AACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAG                                                                        <  SRR4302542.460734/94‑1 (MQ=60)
                       AACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAG                                                                        >  SRR4302542.4260352/1‑94 (MQ=60)
                       AACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAG                                                                        >  SRR4302542.6394209/1‑94 (MQ=60)
                        ACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGT                                                                       >  SRR4302542.1533987/1‑94 (MQ=60)
                        ACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGT                                                                       <  SRR4302542.659326/94‑1 (MQ=60)
                        ACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGT                                                                       >  SRR4302542.1417307/1‑94 (MQ=60)
                         CTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTG                                                                      <  SRR4302542.2178259/94‑1 (MQ=60)
                          TTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTGA                                                                     <  SRR4302542.2898738/94‑1 (MQ=60)
                          TTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTGA                                                                     <  SRR4302542.3044943/94‑1 (MQ=60)
                           TAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGAT                                                                    >  SRR4302542.6469838/1‑94 (MQ=60)
                           TAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGAT                                                                    >  SRR4302542.5193608/1‑94 (MQ=60)
                            AATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATC                                                                   <  SRR4302542.2809458/94‑1 (MQ=60)
                             ATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCG                                                                  >  SRR4302542.609202/1‑94 (MQ=60)
                                ACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCT                                                               >  SRR4302542.2301416/1‑94 (MQ=60)
                                ACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCT                                                               <  SRR4302542.4262045/94‑1 (MQ=60)
                                ACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCT                                                               <  SRR4302542.6787301/94‑1 (MQ=60)
                                ACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCT                                                               >  SRR4302542.2086060/1‑94 (MQ=60)
                                ACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCT                                                               >  SRR4302542.3234489/1‑94 (MQ=60)
                                 CCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTG                                                              >  SRR4302542.6403479/1‑94 (MQ=60)
                                  CAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCATAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGC                                                             >  SRR4302542.7377127/1‑94 (MQ=60)
                                  CAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGC                                                             >  SRR4302542.3847382/1‑94 (MQ=60)
                                   AGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCA                                                            >  SRR4302542.3308333/1‑94 (MQ=60)
                                   AGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCA                                                            >  SRR4302542.1211492/1‑94 (MQ=60)
                                    GCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCAT                                                           <  SRR4302542.2119600/94‑1 (MQ=60)
                                     CGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATG                                                          >  SRR4302542.947466/1‑94 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        <  SRR4302542.931108/94‑1 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        >  SRR4302542.7494500/1‑94 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        <  SRR4302542.5132598/94‑1 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        <  SRR4302542.5686369/94‑1 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        <  SRR4302542.2008202/94‑1 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        <  SRR4302542.6756106/94‑1 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        >  SRR4302542.1846211/1‑94 (MQ=60)
                                       CATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTT                                                        >  SRR4302542.4252480/1‑94 (MQ=60)
                                        ATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTG                                                       <  SRR4302542.7601886/94‑1 (MQ=60)
                                        ATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTG                                                       >  SRR4302542.5074082/1‑94 (MQ=60)
                                        ATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTG                                                       <  SRR4302542.1331646/94‑1 (MQ=60)
                                        ATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTG                                                       >  SRR4302542.6983879/1‑94 (MQ=60)
                                        ATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTG                                                       >  SRR4302542.1682785/1‑94 (MQ=60)
                                         TTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGG                                                      <  SRR4302542.5025464/94‑1 (MQ=60)
                                          TGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGC                                                     <  SRR4302542.2218598/94‑1 (MQ=60)
                                          TGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGC                                                     >  SRR4302542.6109074/1‑94 (MQ=60)
                                          TGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGC                                                     >  SRR4302542.2559102/1‑94 (MQ=60)
                                          TGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGC                                                     <  SRR4302542.2857313/94‑1 (MQ=60)
                                           GACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCG                                                    <  SRR4302542.4125388/94‑1 (MQ=60)
                                           GACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCG                                                    <  SRR4302542.2505973/94‑1 (MQ=60)
                                           GACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCG                                                    >  SRR4302542.89106/1‑94 (MQ=60)
                                            ACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGA                                                   <  SRR4302542.2277115/94‑1 (MQ=60)
                                            ACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGA                                                   <  SRR4302542.251122/94‑1 (MQ=60)
                                            ACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGA                                                   >  SRR4302542.2617163/1‑94 (MQ=60)
                                            ACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGA                                                   <  SRR4302542.3065897/94‑1 (MQ=60)
                                             CGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGAT                                                  >  SRR4302542.2731096/1‑94 (MQ=60)
                                             CGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGAT                                                  >  SRR4302542.6736662/1‑94 (MQ=60)
                                              GATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATT                                                 >  SRR4302542.405568/1‑94 (MQ=60)
                                              GATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATT                                                 >  SRR4302542.1310874/1‑94 (MQ=60)
                                               ATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTG                                                <  SRR4302542.1341584/94‑1 (MQ=60)
                                                TATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGC                                               >  SRR4302542.2275835/1‑94 (MQ=60)
                                                  TCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCG                                             <  SRR4302542.219135/94‑1 (MQ=60)
                                                    GATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTT                                           <  SRR4302542.519348/94‑1 (MQ=60)
                                                    GATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTT                                           >  SRR4302542.6706425/1‑94 (MQ=60)
                                                    GATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTT                                           >  SRR4302542.1095937/1‑94 (MQ=60)
                                                    GATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTT                                           <  SRR4302542.2939746/94‑1 (MQ=60)
                                                     ATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTG                                          <  SRR4302542.4256693/94‑1 (MQ=60)
                                                     ATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTG                                          >  SRR4302542.4499297/1‑94 (MQ=60)
                                                     ATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTG                                          <  SRR4302542.6904219/94‑1 (MQ=60)
                                                     ATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTG                                          >  SRR4302542.1396735/1‑94 (MQ=60)
                                                     ATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTG                                          >  SRR4302542.1883265/1‑94 (MQ=60)
                                                      TAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGG                                         <  SRR4302542.3759767/94‑1 (MQ=60)
                                                       AAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGC                                        >  SRR4302542.5424055/1‑94 (MQ=60)
                                                       AAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGC                                        <  SRR4302542.2087832/94‑1 (MQ=60)
                                                       AAAGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGC                                        >  SRR4302542.5649967/1‑94 (MQ=60)
                                                         AGAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGT                                      >  SRR4302542.4347954/1‑94 (MQ=60)
                                                         AGAACGCACCGACCATCGGCACCACCAAAAACGCCACGCGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGT                                      <  SRR4302542.6393324/94‑1 (MQ=60)
                                                          GAACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTT                                     <  SRR4302542.1413514/94‑1 (MQ=60)
                                                          GAACGCACCGACCATCGGCACCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTT                                     <  SRR4302542.4368321/94‑1 (MQ=60)
                                                           AACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTG                                    >  SRR4302542.4519106/1‑94 (MQ=60)
                                                           AACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTG                                    >  SRR4302542.6678230/1‑94 (MQ=60)
                                                           AACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTG                                    <  SRR4302542.7401376/94‑1 (MQ=60)
                                                           AACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTG                                    >  SRR4302542.4308411/1‑94 (MQ=60)
                                                            ACGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGC                                   >  SRR4302542.5707181/1‑94 (MQ=60)
                                                             CGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCA                                  <  SRR4302542.7249769/94‑1 (MQ=60)
                                                             CGCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCA                                  >  SRR4302542.3287877/1‑94 (MQ=60)
                                                              GCACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCAC                                 >  SRR4302542.7520664/1‑94 (MQ=60)
                                                               CACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACC                                >  SRR4302542.6973390/1‑94 (MQ=60)
                                                                ACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCG                               >  SRR4302542.1466886/1‑94 (MQ=60)
                                                                ACCGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCG                               >  SRR4302542.7411957/1‑94 (MQ=60)
                                                                  CGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAG                             <  SRR4302542.6543893/94‑1 (MQ=60)
                                                                  CGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAG                             <  SRR4302542.6531458/94‑1 (MQ=60)
                                                                  CGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAG                             >  SRR4302542.680842/1‑94 (MQ=60)
                                                                  CGACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAG                             <  SRR4302542.5387868/94‑1 (MQ=60)
                                                                   GACCATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGG                            <  SRR4302542.6570761/94‑1 (MQ=60)
                                                                    ACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGC                           <  SRR4302542.34055/94‑1 (MQ=60)
                                                                       ATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAA                        <  SRR4302542.713630/94‑1 (MQ=60)
                                                                       ATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAA                        >  SRR4302542.914984/1‑94 (MQ=60)
                                                                       ATCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAA                        >  SRR4302542.1281256/1‑94 (MQ=60)
                                                                        TCGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAA                       <  SRR4302542.7231470/94‑1 (MQ=60)
                                                                         CGGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAA                      >  SRR4302542.1297497/1‑94 (MQ=60)
                                                                          GGCACCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAAC                     <  SRR4302542.3565924/94‑1 (MQ=60)
                                                                             ACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCAC                  >  SRR4302542.6133496/1‑94 (MQ=60)
                                                                              CCACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACA                 <  SRR4302542.5958733/94‑1 (MQ=60)
                                                                              CCACCAAAAACACCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCATTGGCGTTGCACCGAGGCCAAAACCACA                 >  SRR4302542.7171918/1‑94 (MQ=60)
                                                                               CACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAG                <  SRR4302542.3069919/94‑1 (MQ=60)
                                                                               CACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAG                >  SRR4302542.5055159/1‑94 (MQ=60)
                                                                               CACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAG                >  SRR4302542.3550354/1‑94 (MQ=60)
                                                                                ACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGT               >  SRR4302542.2799578/1‑94 (MQ=60)
                                                                                ACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGT               >  SRR4302542.5019718/1‑94 (MQ=60)
                                                                                ACCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGT               <  SRR4302542.4231138/94‑1 (MQ=60)
                                                                                 CCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTG              >  SRR4302542.387660/1‑94 (MQ=60)
                                                                                 CCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTG              >  SRR4302542.3677460/1‑94 (MQ=60)
                                                                                 CCAAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTG              >  SRR4302542.2151005/1‑94 (MQ=60)
                                                                                  CAAAAACGCCATGTGCGACGGGCCAAAGGGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGA             >  SRR4302542.5718211/1‑94 (MQ=60)
                                                                                   AAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAC            >  SRR4302542.4483162/1‑94 (MQ=60)
                                                                                   AAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAC            >  SRR4302542.6634050/1‑94 (MQ=60)
                                                                                   AAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAC            <  SRR4302542.6985353/94‑1 (MQ=60)
                                                                                   AAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAC            >  SRR4302542.6103853/1‑94 (MQ=60)
                                                                                   AAAAACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGAC            <  SRR4302542.5609144/94‑1 (MQ=60)
                                                                                       ACGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGC        >  SRR4302542.2739443/1‑94 (MQ=60)
                                                                                        CGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCA       >  SRR4302542.2027732/1‑94 (MQ=60)
                                                                                        CGCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCA       >  SRR4302542.1758668/1‑94 (MQ=60)
                                                                                        CGCCACGCGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCA       <  SRR4302542.1516962/94‑1 (MQ=60)
                                                                                         GCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAG      >  SRR4302542.636353/1‑94 (MQ=60)
                                                                                         GCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAG      >  SRR4302542.6088946/1‑94 (MQ=60)
                                                                                         GCCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAG      >  SRR4302542.5946232/1‑94 (MQ=60)
                                                                                          CCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGC     >  SRR4302542.2157387/1‑94 (MQ=60)
                                                                                          CCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGC     <  SRR4302542.201601/94‑1 (MQ=60)
                                                                                          CCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGC     >  SRR4302542.6129966/1‑94 (MQ=60)
                                                                                          CCACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGC     >  SRR4302542.1833901/1‑94 (MQ=60)
                                                                                           CATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCC    <  SRR4302542.4004742/94‑1 (MQ=60)
                                                                                           CACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCC    >  SRR4302542.1392708/1‑94 (MQ=60)
                                                                                            ACGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCA   >  SRR4302542.2348935/1‑94 (MQ=60)
                                                                                             CGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAG  >  SRR4302542.3306206/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAAAAATCGGCAACATCAAATACAACTTAATTACCAGCGCATTGACGATATCGATAAAGAACGCACCGACCATCGGCACCACCAAAAACGCCATGTGCGACGGGCCAAAGCGTTCAGTGATCGCCTGCATGTTGGCGATTGCCGTTGGCGTTGCACCGAGGCCAAAACCACAGTGACCCGCAGCCAG  >  NC_000913/3825095‑3825281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑