Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,896,324 +G coding (698/711 nt) b3718 ← putative isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,896,3241.G100.0% 897.0 / NA 264V233A (GTG→GCG) b3718putative isomerase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (143/121);  total (143/121)

Only 100 of 282 total aligned reads displayed.

TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA  >  NC_000913/3896232‑3896410
                                                                                             |                                                                                      
  cGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagc                                                                                       <  2:827498/93‑3 (MQ=255)
  cGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagc                                                                                       >  1:4710640/1‑91 (MQ=255)
   gTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcg                                                                                      <  2:2592777/93‑4 (MQ=255)
   gTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcg                                                                                      >  2:5654663/1‑90 (MQ=255)
   gTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcg                                                                                      >  2:3578155/1‑90 (MQ=255)
    tAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcgg                                                                                     <  2:6134863/93‑5 (MQ=255)
       ttATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCtt                                                                                  <  2:6620100/93‑1 (MQ=255)
        tATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCttt                                                                                 >  1:1545243/1‑93 (MQ=255)
         aTTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTa                                                                                >  1:5547136/1‑93 (MQ=255)
         aTTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTa                                                                                <  1:6835171/93‑1 (MQ=255)
           ttAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATc                                                                              <  1:6453454/93‑1 (MQ=255)
           ttAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATc                                                                              >  1:3273620/1‑93 (MQ=255)
            tAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                                                                             <  1:4266113/93‑1 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           >  1:7351135/1‑93 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           <  1:3991421/93‑1 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           <  2:1855366/93‑1 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           >  2:2212005/1‑93 (MQ=255)
               ataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCg                                                                          >  1:1016854/1‑93 (MQ=255)
                 aatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAt                                                                        >  2:3185087/1‑93 (MQ=255)
                 aatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAt                                                                        >  2:5283778/1‑93 (MQ=255)
                 aatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAt                                                                        >  2:6919461/1‑93 (MQ=255)
                 aatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAt                                                                        >  1:4647399/1‑93 (MQ=255)
                   tGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTa                                                                      >  1:5272346/1‑93 (MQ=255)
                    gagaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAc                                                                     >  1:2196692/1‑93 (MQ=255)
                     agaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                                    <  2:2756302/93‑1 (MQ=255)
                     agaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                                    >  2:363774/1‑93 (MQ=255)
                     agaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                                    <  2:124189/93‑1 (MQ=255)
                     agaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                                    <  1:3625971/93‑1 (MQ=255)
                        aCAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATc                                                                 >  2:5213689/1‑93 (MQ=255)
                           ggCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGc                                                              >  2:6713418/1‑93 (MQ=255)
                           ggCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGc                                                              <  2:6485945/93‑1 (MQ=255)
                             ccGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGa                                                            <  2:4849624/93‑1 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  1:2625536/1‑93 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAACTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  1:5821655/1‑93 (MQ=255)
                                gAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAgg                                                         <  1:1186139/93‑1 (MQ=255)
                                gAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAgg                                                         >  1:5912835/1‑93 (MQ=255)
                                  gCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAt                                                       <  2:2132105/93‑1 (MQ=255)
                                   cGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATg                                                      <  1:5654926/93‑1 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     >  2:117310/1‑93 (MQ=255)
                                     tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCa                                                    >  2:7040150/1‑93 (MQ=255)
                                     tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCa                                                    >  1:3500052/1‑93 (MQ=255)
                                       aTTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGc                                                  >  2:4306056/1‑93 (MQ=255)
                                       aTTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGc                                                  >  2:1063988/1‑93 (MQ=255)
                                           acacaTCCGGCCTTATTTCTTAAGCTTAACAGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCa                                              >  1:2407077/1‑93 (MQ=255)
                                            cacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCaa                                             >  1:2874181/1‑93 (MQ=255)
                                              caTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAaca                                           <  1:2539726/93‑1 (MQ=255)
                                               aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAacac                                          <  1:3847093/93‑1 (MQ=255)
                                                tCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACg                                         >  2:453496/1‑93 (MQ=255)
                                                tCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACg                                         >  2:2480756/1‑93 (MQ=255)
                                                  cGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGa                                       <  1:4559518/93‑1 (MQ=255)
                                                  cGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGa                                       >  1:5984178/1‑93 (MQ=255)
                                                   ggCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGaa                                      <  1:6354443/93‑1 (MQ=255)
                                                   ggCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGaa                                      <  2:1917679/93‑1 (MQ=255)
                                                    gCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAg                                     <  1:1838444/93‑1 (MQ=255)
                                                     ccTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGc                                    >  1:654963/1‑93 (MQ=255)
                                                      cTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCt                                   >  1:4800533/1‑93 (MQ=255)
                                                      cTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCt                                   <  2:2223556/93‑1 (MQ=255)
                                                      cTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCt                                   >  2:3897965/1‑93 (MQ=255)
                                                      cTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCt                                   <  2:2913801/93‑1 (MQ=255)
                                                       ttATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTg                                  >  2:3749699/1‑93 (MQ=255)
                                                          tttCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGaa                               >  1:4433671/1‑93 (MQ=255)
                                                            tCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACa                             <  2:384051/93‑1 (MQ=255)
                                                             cTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACAt                            <  2:5053541/93‑1 (MQ=255)
                                                              ttAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATc                           >  2:1810623/1‑93 (MQ=255)
                                                              ttAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATc                           <  1:2148257/93‑1 (MQ=255)
                                                              ttAAGCTTAACCCAGTGCTAATTCTGCGGCAGCGGCTTTATTAGCGATTACCATCACCGAAGGATGTAGCTGCAACACGGTAGCTGGAACAtt                           >  1:946499/1‑92 (MQ=255)
                                                               tAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCt                          <  1:7205567/93‑1 (MQ=255)
                                                                aaGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCtt                         >  2:3493456/1‑93 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGTGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        >  1:5013675/1‑93 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        >  2:2071963/1‑93 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        <  1:1320897/93‑1 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        <  2:5163076/93‑1 (MQ=255)
                                                                  gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCg                       >  1:412017/1‑93 (MQ=255)
                                                                   cTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCgg                      >  1:1061559/1‑93 (MQ=255)
                                                                   cTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCgg                      <  1:3105566/93‑1 (MQ=255)
                                                                   cTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCgg                      <  2:3114333/93‑1 (MQ=255)
                                                                   cTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCgg                      <  1:886636/93‑1 (MQ=255)
                                                                    ttAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGt                     >  1:6909154/1‑93 (MQ=255)
                                                                    ttAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGt                     >  2:6457550/1‑93 (MQ=255)
                                                                    ttAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGt                     >  2:6373419/1‑93 (MQ=255)
                                                                     tAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTa                    >  1:3136791/1‑93 (MQ=255)
                                                                      aaCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTaa                   >  2:4231314/1‑93 (MQ=255)
                                                                       aCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAc                  >  2:155406/1‑93 (MQ=255)
                                                                        ccGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAcc                 >  2:7164423/1‑93 (MQ=255)
                                                                          gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCgg               >  1:5617403/1‑93 (MQ=255)
                                                                          gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCgg               <  2:681459/93‑1 (MQ=255)
                                                                            gTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAc             >  1:1773912/1‑93 (MQ=255)
                                                                            gTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAc             >  2:5417290/1‑93 (MQ=255)
                                                                             tGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAcc            <  2:603909/93‑1 (MQ=255)
                                                                               cTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCtt          <  2:4699459/93‑1 (MQ=255)
                                                                               cTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCtt          <  1:7444306/93‑1 (MQ=255)
                                                                                 aaTTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGc        >  1:6657409/1‑93 (MQ=255)
                                                                                  aTTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCa       >  1:6132176/1‑93 (MQ=255)
                                                                                  aTTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCa       >  1:4101385/1‑93 (MQ=255)
                                                                                   ttCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAg      >  1:5710688/1‑93 (MQ=255)
                                                                                   ttCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAg      <  1:1991540/93‑1 (MQ=255)
                                                                                   ttCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAg      >  2:1457320/1‑93 (MQ=255)
                                                                                      tGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAc   <  2:4091976/93‑1 (MQ=255)
                                                                                      tGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAc   >  1:2999355/1‑93 (MQ=255)
                                                                                       gCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACa  <  2:2983288/93‑1 (MQ=255)
                                                                                             |                                                                                      
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA  >  NC_000913/3896232‑3896410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  >  NC_000913/3896232‑3896417
                                                                                            |                                                                                              
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑G                                                                                              <  SRR4302542.393814/94‑1 (MQ=60)
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑G                                                                                              >  SRR4302542.3901212/1‑94 (MQ=60)
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑G                                                                                              >  SRR4302542.4522321/1‑94 (MQ=60)
 TCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgc                                                                                              >  SRR4302542.219114/1‑92 (MQ=60)
 TCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgc                                                                                              <  SRR4302542.3436315/94‑3 (MQ=60)
 TCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgc                                                                                              >  SRR4302542.4756580/1‑92 (MQ=60)
 TCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgc                                                                                              >  SRR4302542.4756581/1‑92 (MQ=60)
  CGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgcg                                                                                             <  SRR4302542.3072974/94‑4 (MQ=60)
   GTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGG                                                                                            >  SRR4302542.3775726/1‑94 (MQ=60)
   GTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGG                                                                                            >  SRR4302542.4213296/1‑94 (MQ=60)
     AGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCT                                                                                          >  SRR4302542.7336658/1‑94 (MQ=60)
     AGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCT                                                                                          <  SRR4302542.4637779/94‑1 (MQ=60)
     AGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCT                                                                                          <  SRR4302542.7008094/94‑1 (MQ=60)
     AGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCT                                                                                          <  SRR4302542.2578683/94‑1 (MQ=60)
       TTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTT                                                                                        >  SRR4302542.1558576/1‑94 (MQ=60)
        TATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTA                                                                                       >  SRR4302542.5601043/1‑94 (MQ=60)
         ATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAT                                                                                      <  SRR4302542.2143189/94‑1 (MQ=60)
         ATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAT                                                                                      <  SRR4302542.6902335/94‑1 (MQ=60)
          TTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATC                                                                                     <  SRR4302542.6599861/94‑1 (MQ=60)
          TTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATC                                                                                     >  SRR4302542.3304507/1‑94 (MQ=60)
           TTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCA                                                                                    >  SRR4302542.1654553/1‑94 (MQ=60)
           TTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCA                                                                                    <  SRR4302542.6516998/94‑1 (MQ=60)
           TTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCA                                                                                    <  SRR4302542.7230038/94‑1 (MQ=60)
            TAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAG                                                                                   <  SRR4302542.4308097/94‑1 (MQ=60)
             AAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGC                                                                                  >  SRR4302542.7423125/1‑94 (MQ=60)
              AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 <  SRR4302542.3135744/94‑1 (MQ=60)
              AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 >  SRR4302542.1025612/1‑94 (MQ=60)
              AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 <  SRR4302542.4030802/94‑1 (MQ=60)
               ATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGA                                                                                >  SRR4302542.4059464/1‑94 (MQ=60)
                TAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAT                                                                               >  SRR4302542.5712744/1‑94 (MQ=60)
                TAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAT                                                                               >  SRR4302542.4692839/1‑94 (MQ=60)
                 AATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATT                                                                              >  SRR4302542.1866427/1‑94 (MQ=60)
                  ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTA                                                                             >  SRR4302542.5323578/1‑94 (MQ=60)
                   TGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAC                                                                            >  SRR4302542.2215608/1‑94 (MQ=60)
                     AGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCA                                                                          <  SRR4302542.3659748/94‑1 (MQ=60)
                     AGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCA                                                                          >  SRR4302542.622554/1‑94 (MQ=60)
                      GAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCAT                                                                         >  SRR4302542.4326140/1‑94 (MQ=60)
                          AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     >  SRR4302542.988778/1‑94 (MQ=60)
                          AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     >  SRR4302542.6732685/1‑94 (MQ=60)
                          AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     <  SRR4302542.6372075/94‑1 (MQ=60)
                          AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     <  SRR4302542.5603418/94‑1 (MQ=60)
                          AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTACGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     >  SRR4302542.3551348/1‑94 (MQ=60)
                           GGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCG                                                                    >  SRR4302542.445323/1‑94 (MQ=60)
                              CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 <  SRR4302542.1199289/94‑1 (MQ=60)
                              CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 >  SRR4302542.4983692/1‑94 (MQ=60)
                              CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 >  SRR4302542.2650902/1‑94 (MQ=60)
                              CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAACTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 >  SRR4302542.5878174/1‑94 (MQ=60)
                               GGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGG                                                                >  SRR4302542.7500874/1‑94 (MQ=60)
                               GGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGG                                                                >  SRR4302542.1450234/1‑94 (MQ=60)
                               GGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGG                                                                >  SRR4302542.5970137/1‑94 (MQ=60)
                                GAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGA                                                               <  SRR4302542.1196366/94‑1 (MQ=60)
                                 AGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAT                                                              <  SRR4302542.4159335/94‑1 (MQ=60)
                                  GCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGTGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATG                                                             <  SRR4302542.1136197/94‑1 (MQ=60)
                                   CGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGC                                                            <  SRR4302542.5709825/94‑1 (MQ=60)
                                    GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302542.6470032/1‑94 (MQ=60)
                                    GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302542.5259527/1‑94 (MQ=60)
                                    GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302542.3532788/1‑94 (MQ=60)
                                    GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           <  SRR4302542.25543/94‑1 (MQ=60)
                                    GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           <  SRR4302542.5895637/94‑1 (MQ=60)
                                    GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           <  SRR4302542.6127194/94‑1 (MQ=60)
                                     TAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAG                                                          >  SRR4302542.6906634/1‑94 (MQ=60)
                                     TAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAG                                                          <  SRR4302542.937890/94‑1 (MQ=60)
                                       ATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCT                                                        >  SRR4302542.1563405/1‑94 (MQ=60)
                                        TTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTG                                                       <  SRR4302542.1058262/94‑1 (MQ=60)
                                        TTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTG                                                       <  SRR4302542.3630840/94‑1 (MQ=60)
                                          CACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCA                                                     >  SRR4302542.2716689/1‑94 (MQ=60)
                                          CACACATCCGGCCTTATTTCTTAAGCTTAACAGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCA                                                     >  SRR4302542.2430494/1‑94 (MQ=60)
                                           ACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAA                                                    >  SRR4302542.2901778/1‑94 (MQ=60)
                                           ACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAA                                                    >  SRR4302542.209074/1‑94 (MQ=60)
                                             ACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA                                                  <  SRR4302542.27637/94‑1 (MQ=60)
                                              CATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACAC                                                 <  SRR4302542.2564350/94‑1 (MQ=60)
                                               ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                <  SRR4302542.3885105/94‑1 (MQ=60)
                                               ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                >  SRR4302542.5139710/1‑94 (MQ=60)
                                               ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                <  SRR4302542.5531326/94‑1 (MQ=60)
                                               ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                <  SRR4302542.569941/94‑1 (MQ=60)
                                                 CCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGA                                              >  SRR4302542.4540095/1‑94 (MQ=60)
                                                 CCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGA                                              >  SRR4302542.2521625/1‑94 (MQ=60)
                                                 CCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGA                                              >  SRR4302542.6761639/1‑94 (MQ=60)
                                                 CCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGA                                              >  SRR4302542.6042147/1‑94 (MQ=60)
                                                  CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             >  SRR4302542.3216424/1‑94 (MQ=60)
                                                  CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             <  SRR4302542.4604166/94‑1 (MQ=60)
                                                  CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             <  SRR4302542.6516290/94‑1 (MQ=60)
                                                  CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             >  SRR4302542.10736/1‑94 (MQ=60)
                                                   GGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAG                                            <  SRR4302542.6417034/94‑1 (MQ=60)
                                                   GGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAG                                            <  SRR4302542.5857537/94‑1 (MQ=60)
                                                   GGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAG                                            <  SRR4302542.3709674/94‑1 (MQ=60)
                                                   GGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAG                                            >  SRR4302542.1791460/1‑94 (MQ=60)
                                                    GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGC                                           >  SRR4302542.660616/1‑94 (MQ=60)
                                                    GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGC                                           <  SRR4302542.1854358/94‑1 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          >  SRR4302542.5622455/1‑94 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          >  SRR4302542.4847295/1‑94 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          >  SRR4302542.4810391/1‑94 (MQ=60)
                                                      CTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTG                                         <  SRR4302542.6609599/94‑1 (MQ=60)
                                                       TTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGG                                        <  SRR4302542.4171630/94‑1 (MQ=60)
                                                        TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       >  SRR4302542.1258275/1‑94 (MQ=60)
                                                         ATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      <  SRR4302542.602916/94‑1 (MQ=60)
                                                         ATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      >  SRR4302542.4477194/1‑94 (MQ=60)
                                                         ATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      <  SRR4302542.6702281/94‑1 (MQ=60)
                                                            TCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACAT                                   <  SRR4302542.3164746/94‑1 (MQ=60)
                                                            TCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACAT                                   <  SRR4302542.2178646/94‑1 (MQ=60)
                                                             CTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATC                                  >  SRR4302542.3038471/1‑94 (MQ=60)
                                                             CTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATC                                  >  SRR4302542.7279648/1‑94 (MQ=60)
                                                              TTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCT                                 <  SRR4302542.2166777/94‑1 (MQ=60)
                                                               TAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTT                                <  SRR4302542.7276284/94‑1 (MQ=60)
                                                                AAGCTTAACCGAGTGCTAATTCTGTGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               >  SRR4302542.5062364/1‑94 (MQ=60)
                                                                AAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               >  SRR4302542.7172357/1‑94 (MQ=60)
                                                                AAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               >  SRR4302542.4728066/1‑94 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302542.1467701/94‑1 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302542.1332271/94‑1 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              >  SRR4302542.415563/1‑94 (MQ=60)
                                                                  GCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGG                             >  SRR4302542.1070684/1‑94 (MQ=60)
                                                                   CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            <  SRR4302542.3135085/94‑1 (MQ=60)
                                                                   CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            >  SRR4302542.6707646/1‑94 (MQ=60)
                                                                   CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            <  SRR4302542.894299/94‑1 (MQ=60)
                                                                   CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            >  SRR4302542.6976983/1‑94 (MQ=60)
                                                                   CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            <  SRR4302542.903241/94‑1 (MQ=60)
                                                                    TTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTA                           >  SRR4302542.3166607/1‑94 (MQ=60)
                                                                    TTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTA                           <  SRR4302542.4673243/94‑1 (MQ=60)
                                                                      AACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAC                         >  SRR4302542.3515102/1‑94 (MQ=60)
                                                                      AACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAC                         <  SRR4302542.795372/94‑1 (MQ=60)
                                                                       ACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACC                        <  SRR4302542.2945866/94‑1 (MQ=60)
                                                                        CCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCG                       <  SRR4302542.2628738/94‑1 (MQ=60)
                                                                         CGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGG                      >  SRR4302542.5671973/1‑94 (MQ=60)
                                                                         CGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGG                      <  SRR4302542.5912559/94‑1 (MQ=60)
                                                                         CCAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGG                      >  SRR4302542.552365/1‑94 (MQ=60)
                                                                          GAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGA                     <  SRR4302542.587063/94‑1 (MQ=60)
                                                                           AGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAC                    >  SRR4302542.1789290/1‑94 (MQ=60)
                                                                           AGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAC                    <  SRR4302542.2984983/94‑1 (MQ=60)
                                                                             TGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCT                  <  SRR4302542.2362020/94‑1 (MQ=60)
                                                                              GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTT                 >  SRR4302542.5995174/1‑94 (MQ=60)
                                                                              GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTT                 >  SRR4302542.4688456/1‑94 (MQ=60)
                                                                               CTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTG                <  SRR4302542.4884982/94‑1 (MQ=60)
                                                                               CTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTG                <  SRR4302542.7517194/94‑1 (MQ=60)
                                                                                TAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGC               >  SRR4302542.6723000/1‑94 (MQ=60)
                                                                                TAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGC               <  SRR4302542.724771/94‑1 (MQ=60)
                                                                                 AATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCA              >  SRR4302542.4141824/1‑94 (MQ=60)
                                                                                 AATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCA              >  SRR4302542.6191497/1‑94 (MQ=60)
                                                                                  ATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAG             >  SRR4302542.5766119/1‑94 (MQ=60)
                                                                                  ATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAG             >  SRR4302542.224543/1‑94 (MQ=60)
                                                                                  ATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAG             <  SRR4302542.744674/94‑1 (MQ=60)
                                                                                   TTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGA            >  SRR4302542.4107476/1‑94 (MQ=60)
                                                                                   TTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGA            <  SRR4302542.2008727/94‑1 (MQ=60)
                                                                                     CTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAC          >  SRR4302542.3028045/1‑94 (MQ=60)
                                                                                      TGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA         <  SRR4302542.1984278/94‑1 (MQ=60)
                                                                                      TGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA         <  SRR4302542.5752497/94‑1 (MQ=60)
                                                                                       GCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACAT        <  SRR4302542.761878/94‑1 (MQ=60)
                                                                                         GGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTT      >  SRR4302542.1811771/1‑94 (MQ=60)
                                                                                         GGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTT      >  SRR4302542.2946492/1‑94 (MQ=60)
                                                                                          GCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTT     <  SRR4302542.1316404/94‑1 (MQ=60)
                                                                                            G‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  >  SRR4302542.4240014/1‑94 (MQ=60)
                                                                                            |                                                                                              
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  >  NC_000913/3896232‑3896417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑