Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,944,676 2 bp→GC intergenic (+20/+75) trpT → / ← yifDA tRNA‑Trp/transcription regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,944,6760CG99.5% 1498.9 / NA 438intergenic (+20/+76)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (200/236);  total (200/238)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,944,6770GC99.5% 1496.3 / NA 438intergenic (+21/+75)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (200/236);  minor base T (0/2);  total (200/238)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 438 total aligned reads displayed.

GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAACGTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA  >  NC_000913/3944590‑3944764
                                                                                       ||                                                                                        
gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAgg                                                                                     >  2:2845619/1‑93 (MQ=255)
 ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGa                                                                                    <  1:3287775/93‑1 (MQ=255)
 ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGa                                                                                    >  1:300887/1‑93 (MQ=255)
 ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGa                                                                                    <  2:5469318/93‑1 (MQ=255)
  tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑t                                                                                  <  2:2413055/93‑1 (MQ=255)
   cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑tt                                                                                 >  1:4810242/1‑93 (MQ=255)
   cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑tt                                                                                 <  2:4416904/93‑1 (MQ=255)
   cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑tt                                                                                 <  1:2584763/93‑1 (MQ=255)
    aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAATGA‑ttt                                                                                <  1:6688893/93‑1 (MQ=255)
     aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑tttt                                                                               <  1:45642/93‑1 (MQ=255)
     aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑tttt                                                                               <  2:5611274/93‑1 (MQ=255)
     aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑tttt                                                                               <  2:1932642/93‑1 (MQ=255)
      ttGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑ttttt                                                                              >  2:2330838/1‑93 (MQ=255)
         gTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑tttttttt                                                                           <  1:4385284/93‑1 (MQ=255)
          tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTa                                                                          <  2:7445415/93‑1 (MQ=255)
          tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTa                                                                          <  1:6540396/93‑1 (MQ=255)
          tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTa                                                                          <  1:6279368/93‑1 (MQ=255)
          tAGAGCACCGAGCTCCAAATCCGGGTGTTGGGATTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTATGGA‑TTTTTTTTa                                                                          <  1:5711550/93‑1 (MQ=255)
           agagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTAt                                                                         <  1:6946042/93‑1 (MQ=255)
           agagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTAt                                                                         <  2:2256457/93‑1 (MQ=255)
           agagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTAt                                                                         <  2:3457950/93‑1 (MQ=255)
            gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATc                                                                        >  2:5106902/1‑93 (MQ=255)
            gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCCTAGC‑GAAAGCTAAGGA‑TTTTTTTTATc                                                                        <  2:6425194/93‑1 (MQ=255)
             agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCt                                                                       <  2:2909325/93‑1 (MQ=255)
              gCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTg                                                                      <  2:1342065/93‑1 (MQ=255)
               cACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGa                                                                     <  2:2031285/93‑1 (MQ=255)
                aCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGaa                                                                    >  2:1562765/1‑93 (MQ=255)
                aCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGaa                                                                    <  1:3877133/93‑1 (MQ=255)
                 ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGaaa                                                                   >  2:4069049/1‑93 (MQ=255)
                 ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGaaa                                                                   <  1:2801220/93‑1 (MQ=255)
                 ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGaaa                                                                   <  1:7138107/93‑1 (MQ=255)
                   ggTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATa                                                                 <  2:26670/93‑1 (MQ=255)
                    gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATaa                                                                >  1:4961846/1‑93 (MQ=255)
                    gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATaa                                                                >  2:778839/1‑93 (MQ=255)
                     tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAAc                                                               >  2:3921579/1‑93 (MQ=255)
                     tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAAc                                                               >  2:4716661/1‑93 (MQ=255)
                       tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAAccc                                                             >  1:7332162/1‑93 (MQ=255)
                        ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCct                                                            <  1:7398037/93‑1 (MQ=255)
                         cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCctc                                                           >  1:1007735/1‑93 (MQ=255)
                         cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCctc                                                           >  2:6681178/1‑93 (MQ=255)
                          aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCctct                                                          >  1:3467755/1‑93 (MQ=255)
                            aaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTcc                                                        <  2:3387999/93‑1 (MQ=255)
                            aaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTcc                                                        <  1:3964246/93‑1 (MQ=255)
                                gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAg                                                    >  1:5521024/1‑93 (MQ=255)
                                 ggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTTTTTTGAAAAAACCCCTCCCGAAg                                                    >  1:6195926/1‑93 (MQ=255)
                                  gtgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTa                                                  >  2:6362306/1‑93 (MQ=255)
                                    nntGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                                <  1:3716809/91‑1 (MQ=255)
                                    gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                                <  2:4811182/93‑1 (MQ=255)
                                     ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                               <  2:6867640/93‑1 (MQ=255)
                                     ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                               <  2:7174741/93‑1 (MQ=255)
                                     ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                               <  1:7472360/93‑1 (MQ=255)
                                     ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                               <  1:1271339/93‑1 (MQ=255)
                                       gggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATcc                                             <  1:4730061/93‑1 (MQ=255)
                                        ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCt                                            >  2:5262873/1‑93 (MQ=255)
                                        ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCt                                            <  1:298069/93‑1 (MQ=255)
                                          aGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTc                                          <  2:1029174/93‑1 (MQ=255)
                                          aGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTc                                          <  1:1696482/93‑1 (MQ=255)
                                           gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCt                                         >  2:1703465/1‑93 (MQ=255)
                                            ttCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                        <  2:3876103/93‑1 (MQ=255)
                                             tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAc                                       <  1:2015940/93‑1 (MQ=255)
                                              cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAcc                                      <  2:5892373/93‑1 (MQ=255)
                                              cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAcc                                      <  2:114455/93‑1 (MQ=255)
                                               gAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCg                                     >  1:1491277/1‑93 (MQ=255)
                                               gAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCg                                     >  2:7287099/1‑93 (MQ=255)
                                                 gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                   <  2:2773084/93‑1 (MQ=255)
                                                  tctctcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                  <  2:5815467/93‑1 (MQ=255)
                                                  tctctcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                  >  2:4148619/1‑93 (MQ=255)
                                                  tctctcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                  >  2:610916/1‑93 (MQ=255)
                                                    tctcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                                <  1:6094176/93‑1 (MQ=255)
                                                      tcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                              <  1:175643/93‑1 (MQ=255)
                                                      tcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                              <  1:5424542/93‑1 (MQ=255)
                                                      tcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                              >  2:655845/1‑93 (MQ=255)
                                                      tcCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                              >  1:3783785/1‑93 (MQ=255)
                                                      tcCCCCCCTGCCAAAAATCCACCTTAGCGGAAACTAAAGGT‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                               <  1:6375446/93‑1 (MQ=255)
                                                        cGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                            >  1:5907044/1‑93 (MQ=255)
                                                          ccccTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                          <  1:1601951/93‑1 (MQ=255)
                                                          ccccTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                          <  2:5067031/93‑1 (MQ=255)
                                                           cccTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                         <  1:2046072/93‑1 (MQ=255)
                                                           cccTGCCAAAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                         <  1:4132887/93‑1 (MQ=255)
                                                            ccTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                        >  1:4482703/1‑93 (MQ=255)
                                                            ccTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                        >  2:2566210/1‑93 (MQ=255)
                                                              tGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcc                      >  2:426081/1‑93 (MQ=255)
                                                                ccAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAt                    <  1:2403913/93‑1 (MQ=255)
                                                                 cAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAtt                   <  2:4939509/93‑1 (MQ=255)
                                                                 cAGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAtt                   >  1:2106347/1‑93 (MQ=255)
                                                                  aGAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTc                  >  1:2220683/1‑93 (MQ=255)
                                                                   gAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                 >  1:985758/1‑93 (MQ=255)
                                                                   gAAATCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                 >  1:6829209/1‑93 (MQ=255)
                                                                     aaTCATCCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata               <  2:7100625/93‑1 (MQ=255)
                                                                          tcCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt          >  2:3091010/1‑93 (MQ=255)
                                                                          tcCTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt          <  1:5280407/93‑1 (MQ=255)
                                                                           ccTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATa         >  1:4328329/1‑93 (MQ=255)
                                                                            cTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac        <  2:4614053/93‑1 (MQ=255)
                                                                            cTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac        <  1:3188761/93‑1 (MQ=255)
                                                                            cTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac        <  1:3560383/93‑1 (MQ=255)
                                                                            cTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac        >  1:5095624/1‑93 (MQ=255)
                                                                            cTTAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac        >  1:5815390/1‑93 (MQ=255)
                                                                              tAGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac      <  1:254628/93‑1 (MQ=255)
                                                                               aGC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACt     <  2:3645271/93‑1 (MQ=255)
                                                                                gC‑GAAAGCTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt    <  2:3207748/93‑1 (MQ=255)
                                                                                       ||                                                                                        
GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAACGTAAGGA‑TTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA  >  NC_000913/3944590‑3944764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 244 total aligned reads displayed.
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944583‑3944765
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GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           <  SRR4302542.1732919/94‑1 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           <  SRR4302542.52934/94‑1 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           >  SRR4302542.6032727/1‑94 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           <  SRR4302542.3730417/94‑1 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           <  SRR4302542.3288993/94‑1 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           >  SRR4302542.1991078/1‑94 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           <  SRR4302542.5472254/94‑1 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           <  SRR4302542.5769389/94‑1 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAG                                                                                           <  SRR4302542.393242/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAG                                                                                      <  SRR4302542.6647213/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAG                                                                                      <  SRR4302542.6258511/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAG                                                                                      <  SRR4302542.5608788/94‑1 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGG                                                                                     <  SRR4302542.567278/94‑1 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGG                                                                                     <  SRR4302542.5280977/94‑1 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGG                                                                                     >  SRR4302542.1970209/1‑94 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA                                                                                    >  SRR4302542.7226883/1‑94 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA                                                                                    >  SRR4302542.303509/1‑94 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGA                                                                                    >  SRR4302542.3758544/1‑94 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGAT                                                                                   >  SRR4302542.3013201/1‑94 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGAT                                                                                   <  SRR4302542.7225017/94‑1 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGAT                                                                                   <  SRR4302542.3318820/94‑1 (MQ=60)
         TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATT                                                                                  <  SRR4302542.6599941/94‑1 (MQ=60)
         TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATT                                                                                  >  SRR4302542.4857097/1‑94 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTT                                                                                 >  SRR4302542.4453863/1‑94 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTT                                                                                 >  SRR4302542.301312/1‑94 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTT                                                                                 <  SRR4302542.2609802/94‑1 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTT                                                                                 >  SRR4302542.4732526/1‑94 (MQ=60)
           AATTGGTAGAGCTCCGGTCTCCAAAGCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTT                                                                                <  SRR4302542.484839/94‑1 (MQ=60)
           AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAATGATTTT                                                                                <  SRR4302542.6754784/94‑1 (MQ=60)
           AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTT                                                                                <  SRR4302542.2026827/94‑1 (MQ=60)
           AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTT                                                                                >  SRR4302542.2070071/1‑94 (MQ=60)
            ATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTT                                                                               >  SRR4302542.2074019/1‑94 (MQ=60)
            ATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTT                                                                               <  SRR4302542.46029/94‑1 (MQ=60)
            ATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTT                                                                               >  SRR4302542.5473936/1‑94 (MQ=60)
            ATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTT                                                                               <  SRR4302542.7534772/94‑1 (MQ=60)
             TTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTT                                                                              <  SRR4302542.2836443/94‑1 (MQ=60)
              TGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTT                                                                             <  SRR4302542.332317/94‑1 (MQ=60)
               GGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTT                                                                            >  SRR4302542.5807744/1‑94 (MQ=60)
               GGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTT                                                                            <  SRR4302542.1770990/94‑1 (MQ=60)
                GTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTA                                                                           <  SRR4302542.4428310/94‑1 (MQ=60)
                GTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTA                                                                           <  SRR4302542.6144212/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302542.6341248/94‑1 (MQ=60)
                  AGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATC                                                                         <  SRR4302542.7014289/94‑1 (MQ=60)
                  AGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATC                                                                         >  SRR4302542.3610345/1‑94 (MQ=60)
                   GAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCT                                                                        <  SRR4302542.4434008/94‑1 (MQ=60)
                    AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTG                                                                       <  SRR4302542.4384555/94‑1 (MQ=60)
                    AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTG                                                                       >  SRR4302542.4186019/1‑94 (MQ=60)
                    AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTG                                                                       <  SRR4302542.20529/94‑1 (MQ=60)
                     GCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGA                                                                      >  SRR4302542.4668812/1‑94 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAA                                                                     >  SRR4302542.6053916/1‑94 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAA                                                                     <  SRR4302542.6885491/94‑1 (MQ=60)
                       GCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    <  SRR4302542.2544236/94‑1 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGTGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    <  SRR4302542.1079516/94‑1 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    <  SRR4302542.3915434/94‑1 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    <  SRR4302542.6988357/94‑1 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    >  SRR4302542.5309154/1‑94 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    <  SRR4302542.59341/94‑1 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302542.7521710/94‑1 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302542.7208160/94‑1 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302542.2910862/94‑1 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302542.2828197/94‑1 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  <  SRR4302542.2175429/94‑1 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  <  SRR4302542.2373425/94‑1 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  >  SRR4302542.1299736/1‑94 (MQ=60)
                          GGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAA                                                                 <  SRR4302542.2075242/94‑1 (MQ=60)
                           GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                                <  SRR4302542.305236/94‑1 (MQ=60)
                           GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                                >  SRR4302542.5232403/1‑94 (MQ=60)
                            TCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACC                                                               <  SRR4302542.4595900/94‑1 (MQ=60)
                            TCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACC                                                               >  SRR4302542.3793890/1‑94 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              >  SRR4302542.7403989/1‑94 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              <  SRR4302542.5332021/94‑1 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              >  SRR4302542.3285524/1‑94 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              <  SRR4302542.679570/94‑1 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              <  SRR4302542.6480717/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302542.620773/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302542.1580315/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            >  SRR4302542.1016417/1‑94 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302542.5039916/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            >  SRR4302542.5682547/1‑94 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            >  SRR4302542.6961114/1‑94 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302542.7470438/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302542.6217648/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           >  SRR4302542.3500228/1‑94 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           >  SRR4302542.5854522/1‑94 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302542.4089519/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302542.131150/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302542.1031599/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302542.4003391/94‑1 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     >  SRR4302542.6690851/1‑94 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     >  SRR4302542.5574677/1‑94 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     <  SRR4302542.2378385/94‑1 (MQ=60)
                                       GGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTtttttgaaaaaacccctcccgaag                                                    >  SRR4302542.6256340/1‑70 (MQ=60)
                                        GGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTA                                                   <  SRR4302542.5777471/94‑1 (MQ=60)
                                        GGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTA                                                   <  SRR4302542.1649210/94‑1 (MQ=60)
                                          TGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAA                                                 <  SRR4302542.4140124/94‑1 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                <  SRR4302542.5545836/94‑1 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                <  SRR4302542.4914342/94‑1 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                <  SRR4302542.686300/94‑1 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                >  SRR4302542.3509155/1‑94 (MQ=60)
                                            TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                               <  SRR4302542.7545579/94‑1 (MQ=60)
                                            TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                               <  SRR4302542.1282259/94‑1 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              <  SRR4302542.5589363/94‑1 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              <  SRR4302542.6943693/94‑1 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              <  SRR4302542.5370030/94‑1 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              <  SRR4302542.7151117/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAGGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             >  SRR4302542.7445350/1‑94 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             >  SRR4302542.7537518/1‑94 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302542.4776189/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302542.451999/94‑1 (MQ=60)
                                               GGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTT                                            >  SRR4302542.7301087/1‑94 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302542.2113449/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302542.3506470/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302542.1711182/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          >  SRR4302542.5893573/1‑94 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                         >  SRR4302542.3734347/1‑94 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                         >  SRR4302542.2862667/1‑94 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                         <  SRR4302542.1000899/94‑1 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                         <  SRR4302542.633359/94‑1 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                         >  SRR4302542.2507371/1‑94 (MQ=60)
                                                    TCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACC                                       <  SRR4302542.2033345/94‑1 (MQ=60)
                                                    TCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACC                                       >  SRR4302542.2416604/1‑94 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      >  SRR4302542.55170/1‑94 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      <  SRR4302542.6476868/94‑1 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      >  SRR4302542.275904/1‑94 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      >  SRR4302542.1504173/1‑94 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      >  SRR4302542.6314122/1‑94 (MQ=60)
                                                       AGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGC                                    >  SRR4302542.3858958/1‑94 (MQ=60)
                                                       AGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGC                                    >  SRR4302542.5779488/1‑94 (MQ=60)
                                                         TCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAT                                  <  SRR4302542.218819/94‑1 (MQ=60)
                                                         TCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAT                                  >  SRR4302542.3325414/1‑94 (MQ=60)
                                                          CTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATC                                 >  SRR4302542.626897/1‑94 (MQ=60)
                                                           TCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCC                                <  SRR4302542.6153162/94‑1 (MQ=60)
                                                           TCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCC                                >  SRR4302542.2893950/1‑94 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               >  SRR4302542.3821215/1‑94 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               >  SRR4302542.4544103/1‑94 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302542.177130/94‑1 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302542.7504377/94‑1 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302542.2051688/94‑1 (MQ=60)
                                                             TCCCCCCCTGCCAAAAATCCACCTTAGCGGAAACTAAAGGTTTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               <  SRR4302542.6438223/94‑1 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             >  SRR4302542.5964286/1‑94 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             >  SRR4302542.2614740/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCGTCCTTGC                            >  SRR4302542.4619100/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302542.5111497/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302542.5071186/1‑94 (MQ=60)
                                                                GCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCC                           >  SRR4302542.3037144/1‑94 (MQ=60)
                                                                GCCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCC                           >  SRR4302542.2164819/1‑94 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          <  SRR4302542.1516573/94‑1 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          <  SRR4302542.1615786/94‑1 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          >  SRR4302542.5225973/1‑94 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302542.3559941/94‑1 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302542.1449616/94‑1 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302542.2063758/94‑1 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302542.3204682/94‑1 (MQ=60)
                                                                  CCCTGCCAAAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302542.4173610/94‑1 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                        >  SRR4302542.864518/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                        >  SRR4302542.1007439/1‑94 (MQ=60)
                                                                    CTGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCC                       <  SRR4302542.4898432/94‑1 (MQ=60)
                                                                     TGCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCA                      >  SRR4302542.4605250/1‑94 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     >  SRR4302542.3858973/1‑94 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     <  SRR4302542.4384654/94‑1 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     <  SRR4302542.6517729/94‑1 (MQ=60)
                                                                       CCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATT                    <  SRR4302542.2427310/94‑1 (MQ=60)
                                                                       CCAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATT                    >  SRR4302542.2124527/1‑94 (MQ=60)
                                                                        CAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTC                   <  SRR4302542.6191437/94‑1 (MQ=60)
                                                                        CAGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTC                   <  SRR4302542.1758829/94‑1 (MQ=60)
                                                                         AGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  >  SRR4302542.5516675/1‑94 (MQ=60)
                                                                         AGAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  >  SRR4302542.1654317/1‑94 (MQ=60)
                                                                          GAAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCAT                 >  SRR4302542.4154930/1‑94 (MQ=60)
                                                                           AAATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA                >  SRR4302542.4490567/1‑94 (MQ=60)
                                                                            AATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               >  SRR4302542.2570111/1‑94 (MQ=60)
                                                                            AATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302542.4234726/94‑1 (MQ=60)
                                                                             ATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATT              >  SRR4302542.4796894/1‑94 (MQ=60)
                                                                             ATCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATT              <  SRR4302542.3223920/94‑1 (MQ=60)
                                                                              TCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA             >  SRR4302542.5919060/1‑94 (MQ=60)
                                                                              TCATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA             >  SRR4302542.3947396/1‑94 (MQ=60)
                                                                               CATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAA            >  SRR4302542.362973/1‑94 (MQ=60)
                                                                                ATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302542.6852972/1‑94 (MQ=60)
                                                                                ATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302542.5554623/1‑94 (MQ=60)
                                                                                ATCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302542.4877188/1‑94 (MQ=60)
                                                                                 TCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA          <  SRR4302542.3762977/94‑1 (MQ=60)
                                                                                 TCCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA          >  SRR4302542.4370881/1‑94 (MQ=60)
                                                                                  CCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         >  SRR4302542.7414491/1‑94 (MQ=60)
                                                                                  CCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         <  SRR4302542.2590623/94‑1 (MQ=60)
                                                                                  CCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         >  SRR4302542.1902730/1‑94 (MQ=60)
                                                                                  CCTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         <  SRR4302542.3153444/94‑1 (MQ=60)
                                                                                   CTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        >  SRR4302542.6657120/1‑94 (MQ=60)
                                                                                   CTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        >  SRR4302542.6377092/1‑94 (MQ=60)
                                                                                   CTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        <  SRR4302542.3218965/94‑1 (MQ=60)
                                                                                   CTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        <  SRR4302542.3593628/94‑1 (MQ=60)
                                                                                   CTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        >  SRR4302542.7130121/1‑94 (MQ=60)
                                                                                   CTTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        <  SRR4302542.80061/94‑1 (MQ=60)
                                                                                    TTAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACAC       >  SRR4302542.3989877/1‑94 (MQ=60)
                                                                                     TAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT      <  SRR4302542.3886970/94‑1 (MQ=60)
                                                                                     TAGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT      <  SRR4302542.256797/94‑1 (MQ=60)
                                                                                      AGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     <  SRR4302542.2604130/94‑1 (MQ=60)
                                                                                      AGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     >  SRR4302542.437411/1‑94 (MQ=60)
                                                                                      AGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     <  SRR4302542.5098377/94‑1 (MQ=60)
                                                                                      AGC‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     >  SRR4302542.974459/1‑94 (MQ=60)
                                                                                        C‑GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   >  SRR4302542.6663751/1‑94 (MQ=60)
                                                                                          GAAACTTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  <  SRR4302542.1543972/94‑1 (MQ=60)
                                                                                              |                                                                                         
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGC‑GAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944583‑3944765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑