Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,957,551 +T intergenic (‑120/‑741) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,957,5501.T99.5% 1390.3 / ‑3.0 374intergenic (‑119/‑742)ppiC/reppeptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base T (183/189);  total (185/189)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

TAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑T‑GC‑TCGCA‑GGT‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT  >  NC_000913/3957470‑3957627
                                                                                   |                                                                                
 aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCA‑GGT‑c‑cc                                                                    <  2:1064051/93‑2 (MQ=255)
  gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAACCGG‑GCTTCGCA‑GGT‑c‑ccc                                                                   >  1:2120238/1‑91 (MQ=255)
   cATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGT‑c‑cctc                                                                  >  2:6100300/1‑90 (MQ=255)
      gATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAg                                                                <  1:2679786/93‑1 (MQ=255)
        tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAggg                                                              >  2:1314386/1‑93 (MQ=255)
         ccGCAGATGCTAACGTAATTGTCAGTCAGGGAGCGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAgggg                                                             >  1:2197695/1‑93 (MQ=255)
          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGga                                                            <  1:4350163/93‑1 (MQ=255)
          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGga                                                            <  2:6333376/93‑1 (MQ=255)
          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGga                                                            <  2:4776150/93‑1 (MQ=255)
          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGga                                                            <  1:857537/93‑1 (MQ=255)
            cAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGgaga                                                          >  2:7186010/1‑93 (MQ=255)
              gATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGaaa                                                        <  2:1131445/93‑1 (MQ=255)
              gATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGaaa                                                        <  1:2269769/93‑1 (MQ=255)
                  cTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGGGAAAACtt                                                    >  1:5360208/1‑93 (MQ=255)
                  cTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGGGAAAACtt                                                    >  1:6527967/1‑93 (MQ=255)
                   tAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTa                                                   >  1:675393/1‑93 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAg                                                  >  2:680651/1‑93 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAg                                                  <  2:6898813/93‑1 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAg                                                  <  1:7242985/93‑1 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAg                                                  >  2:1630710/1‑93 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAg                                                  <  2:3930457/93‑1 (MQ=255)
                     aCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAgg                                                 <  2:6029585/93‑1 (MQ=255)
                     aCGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAgg                                                 >  1:473448/1‑93 (MQ=255)
                     aCGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAgg                                                 >  1:1213898/1‑93 (MQ=255)
                      cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAggg                                                <  2:3597889/93‑1 (MQ=255)
                       gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGc                                               <  1:3125835/93‑1 (MQ=255)
                       gTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGc                                               >  1:4548655/1‑93 (MQ=255)
                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGcc                                              >  2:4290977/1‑93 (MQ=255)
                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGcc                                              >  2:2501188/1‑93 (MQ=255)
                         aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCct                                             <  1:3158558/93‑1 (MQ=255)
                         aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCct                                             >  2:6980436/1‑93 (MQ=255)
                         aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAACCCGGAAAATCCGG‑GCTTCGCAGGGC‑C‑CTCAAGGGGAGAAAACTTAGGGCct                                             >  1:5220372/1‑93 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCctc                                            <  2:4005295/93‑1 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCctc                                            <  1:5772513/93‑1 (MQ=255)
                           ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCctct                                           >  2:2615137/1‑93 (MQ=255)
                           ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCctct                                           <  2:6613070/93‑1 (MQ=255)
                             gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTAt                                         <  2:4718846/93‑1 (MQ=255)
                             gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTAt                                         <  1:4914451/93‑1 (MQ=255)
                              tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  2:3068252/93‑1 (MQ=255)
                              tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  2:6717192/93‑1 (MQ=255)
                              tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  2:508999/93‑1 (MQ=255)
                              tcagtcagGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        >  1:4162063/1‑93 (MQ=255)
                              tcagtcagGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGGGAAAACTTAGGGCCTCTATg                                        >  1:5407780/1‑93 (MQ=255)
                               cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                       <  2:7291736/93‑1 (MQ=255)
                               cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                       <  1:2814822/93‑1 (MQ=255)
                                 gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                     <  1:5578738/93‑1 (MQ=255)
                                 gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                     <  2:1209232/93‑1 (MQ=255)
                                 gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                     <  1:4038328/93‑1 (MQ=255)
                                 gtcagGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                     >  1:3591628/1‑93 (MQ=255)
                                  tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                    >  2:2827864/1‑93 (MQ=255)
                                  tcagGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGCGAAAACTTAGGGCCTCTATggggt                                    >  1:5719863/1‑91 (MQ=255)
                                   cagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTa                                   >  2:4312120/1‑93 (MQ=255)
                                   cagGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGGCTCTATGGGCTa                                   >  1:3844103/1‑93 (MQ=255)
                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                  <  2:4863370/93‑1 (MQ=255)
                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                  <  1:4616628/93‑1 (MQ=255)
                                     gggAGTGGCTGGGACATATATGGCAAAAGTCCGGAAAATCCGG‑GCTTTGCA‑GGTCC‑CCCCAAGGGCGAAAACATAGGGCCCCCATGGGCTACa                                 >  1:324946/1‑93 (MQ=255)
                                      ggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGT‑CGCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                <  2:1739023/93‑1 (MQ=255)
                                      ggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                >  2:2719668/1‑93 (MQ=255)
                                       gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                               <  1:378319/93‑1 (MQ=255)
                                       gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                               <  1:946153/93‑1 (MQ=255)
                                       gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                               <  1:1401120/93‑1 (MQ=255)
                                       gAGTGGCTGGTACATATATGGCAAAAACCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                               >  1:1610075/1‑93 (MQ=255)
                                        aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                              >  2:335884/1‑93 (MQ=255)
                                          tGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                            >  1:6159723/1‑93 (MQ=255)
                                          tGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                            <  2:6432150/93‑1 (MQ=255)
                                           ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                           >  2:1897442/1‑93 (MQ=255)
                                             cTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                         <  2:822713/93‑1 (MQ=255)
                                             cTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                         <  1:5096962/93‑1 (MQ=255)
                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                        <  1:1887430/93‑1 (MQ=255)
                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                        <  1:2435657/93‑1 (MQ=255)
                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                        <  1:6027750/93‑1 (MQ=255)
                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                        <  2:5018700/93‑1 (MQ=255)
                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                        <  2:5844472/93‑1 (MQ=255)
                                               ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                       <  2:741027/93‑1 (MQ=255)
                                               ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                       >  2:4564047/1‑93 (MQ=255)
                                               ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                       <  2:1163584/93‑1 (MQ=255)
                                               ggTACATATAGGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                       <  1:2513800/93‑1 (MQ=255)
                                                gTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                      <  1:1161824/93‑1 (MQ=255)
                                                gTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                      <  1:1814132/93‑1 (MQ=255)
                                                gTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                      <  2:5324563/93‑1 (MQ=255)
                                                  aCATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                    >  2:569953/1‑93 (MQ=255)
                                                  aCATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                    <  2:6223907/93‑1 (MQ=255)
                                                   cATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                   <  1:77240/93‑1 (MQ=255)
                                                   cATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                   <  1:1592737/93‑1 (MQ=255)
                                                   cATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGCCATTATCggg                   >  1:1995076/1‑93 (MQ=255)
                                                       tatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg               >  2:3505696/1‑93 (MQ=255)
                                                       tatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg               >  2:6072201/1‑93 (MQ=255)
                                                         tGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt             <  1:6333020/93‑1 (MQ=255)
                                                          ggCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt            <  1:7080039/93‑1 (MQ=255)
                                                           gCAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta           <  2:5476560/93‑1 (MQ=255)
                                                            cAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat          <  2:3774211/93‑1 (MQ=255)
                                                            cAAAAGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat          <  2:2428958/93‑1 (MQ=255)
                                                             aaaaGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt         >  1:5535530/1‑93 (MQ=255)
                                                             aaaaGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt         >  2:4167343/1‑93 (MQ=255)
                                                                aGCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta      <  1:6906925/93‑1 (MQ=255)
                                                                 gCCCGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat     >  2:2344766/1‑93 (MQ=255)
                                                                   ccGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg   <  2:2133587/93‑1 (MQ=255)
                                                                    cGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt  <  2:3300779/93‑1 (MQ=255)
                                                                    cGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt  <  1:6463072/93‑1 (MQ=255)
                                                                    cGGAAAATCCGG‑GCTTCGCA‑GGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt  <  2:3667786/93‑1 (MQ=255)
                                                                                   |                                                                                
TAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑T‑GC‑TCGCA‑GGT‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT  >  NC_000913/3957470‑3957627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 211 total aligned reads displayed.
TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCA‑GGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATA  >  NC_000913/3957478‑3957643
                                                                           |                                                                                              
  CGCAGATGCTAACGTAATTGTCAGTCAGGGGGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                           >  SRR4302542.1617432/1‑94 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                           <  SRR4302542.864927/94‑1 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                           <  SRR4302542.4392906/94‑1 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                           <  SRR4302542.4825409/94‑1 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                           <  SRR4302542.2853458/94‑1 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                           >  SRR4302542.2594546/1‑94 (MQ=60)
     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAA                                                                        >  SRR4302542.3934579/1‑94 (MQ=60)
     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAA                                                                        >  SRR4302542.1295651/1‑94 (MQ=60)
     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAA                                                                        >  SRR4302542.512056/1‑94 (MQ=60)
     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAA                                                                        >  SRR4302542.5457962/1‑94 (MQ=60)
      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAA                                                                       >  SRR4302542.2196350/1‑94 (MQ=60)
      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAA                                                                       <  SRR4302542.2289292/94‑1 (MQ=60)
       ATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAAC                                                                      >  SRR4302542.4628743/1‑94 (MQ=60)
       ATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAAC                                                                      >  SRR4302542.6302354/1‑94 (MQ=60)
         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTT                                                                    <  SRR4302542.1874242/94‑1 (MQ=60)
         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTT                                                                    >  SRR4302542.6592303/1‑94 (MQ=60)
          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATTGCAAAAACCCGGAAAATCCGGGCCTTGCA‑GGTCCCTCAAGGGGTGAAAACTTA                                                                   >  SRR4302542.785950/1‑94 (MQ=46)
          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTA                                                                   >  SRR4302542.681226/1‑94 (MQ=60)
          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTA                                                                   >  SRR4302542.776450/1‑94 (MQ=60)
           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAG                                                                  <  SRR4302542.3165378/94‑1 (MQ=60)
           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAG                                                                  <  SRR4302542.2497588/94‑1 (MQ=60)
           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAG                                                                  >  SRR4302542.4583965/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                 <  SRR4302542.3077833/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                 <  SRR4302542.7314016/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                 <  SRR4302542.2177749/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                 <  SRR4302542.2160540/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGG                                                                 >  SRR4302542.16203/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGG                                                                 >  SRR4302542.4588368/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                 >  SRR4302542.1224341/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                 >  SRR4302542.2278717/1‑94 (MQ=60)
             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGG                                                                <  SRR4302542.1885128/94‑1 (MQ=60)
             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGG                                                                >  SRR4302542.1947497/1‑94 (MQ=60)
             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGG                                                                <  SRR4302542.5271031/94‑1 (MQ=60)
              CGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGC                                                               >  SRR4302542.3584511/1‑94 (MQ=60)
              CGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGC                                                               >  SRR4302542.4593176/1‑94 (MQ=60)
               GTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCC                                                              <  SRR4302542.3155563/94‑1 (MQ=60)
               GTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCC                                                              <  SRR4302542.3620903/94‑1 (MQ=60)
                TAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGGGCCT                                                             >  SRR4302542.5271137/1‑94 (MQ=60)
                TAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCT                                                             >  SRR4302542.5542508/1‑94 (MQ=60)
                 AATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                            <  SRR4302542.4209041/94‑1 (MQ=60)
                 AATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                            <  SRR4302542.3188543/94‑1 (MQ=60)
                 AATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                            <  SRR4302542.6018100/94‑1 (MQ=60)
                 AATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                            >  SRR4302542.4956697/1‑94 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                           <  SRR4302542.4633040/94‑1 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                           <  SRR4302542.557379/94‑1 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                           <  SRR4302542.5828541/94‑1 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                           <  SRR4302542.2831727/94‑1 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                           >  SRR4302542.5781473/1‑94 (MQ=60)
                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                         >  SRR4302542.4691173/1‑94 (MQ=60)
                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                         <  SRR4302542.961600/94‑1 (MQ=60)
                    TGTCAGTCAGGGAGTGGCTGGGACATATTTGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGGAAACTTAGGGCCTCTAT                                                         >  SRR4302542.5702716/1‑94 (MQ=57)
                    TGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                         >  SRR4302542.5087189/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        <  SRR4302542.4962244/94‑1 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        <  SRR4302542.5601560/94‑1 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        <  SRR4302542.5299314/94‑1 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        <  SRR4302542.6503653/94‑1 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        >  SRR4302542.4138927/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        >  SRR4302542.7093990/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        >  SRR4302542.4203060/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATG                                                        >  SRR4302542.5460309/1‑94 (MQ=57)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                        >  SRR4302542.315253/1‑94 (MQ=57)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                       <  SRR4302542.522764/94‑1 (MQ=60)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                       <  SRR4302542.6566409/94‑1 (MQ=60)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                       <  SRR4302542.1800291/94‑1 (MQ=60)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                       <  SRR4302542.2708145/94‑1 (MQ=60)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                       <  SRR4302542.1308945/94‑1 (MQ=60)
                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                      <  SRR4302542.2029614/94‑1 (MQ=60)
                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                      <  SRR4302542.2378480/94‑1 (MQ=60)
                        AGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGC                                                     >  SRR4302542.3625126/1‑94 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                    <  SRR4302542.4078174/94‑1 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                    <  SRR4302542.3279836/94‑1 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                    <  SRR4302542.5632951/94‑1 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                    >  SRR4302542.3839289/1‑94 (MQ=60)
                         GTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGGCTCTATGGGCT                                                    >  SRR4302542.6592298/1‑94 (MQ=57)
                         GTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCT                                                    >  SRR4302542.6854901/1‑94 (MQ=60)
                          TCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGGCTCTATGGGCTA                                                   >  SRR4302542.3882098/1‑94 (MQ=60)
                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCTAC                                                  >  SRR4302542.838602/1‑94 (MQ=60)
                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                  >  SRR4302542.3738482/1‑94 (MQ=60)
                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCCAC                                                  >  SRR4302542.4754989/1‑94 (MQ=60)
                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                 <  SRR4302542.4661731/94‑1 (MQ=60)
                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                 <  SRR4302542.4754373/94‑1 (MQ=60)
                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                 >  SRR4302542.5666851/1‑94 (MQ=60)
                             GGGGGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAA                                                >  SRR4302542.7187435/1‑94 (MQ=60)
                              GGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCCGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCTACAAA                                               >  SRR4302542.6713552/1‑94 (MQ=60)
                              GGAGTGGCTGGTACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAA                                               >  SRR4302542.1623977/1‑94 (MQ=60)
                              GGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAA                                               >  SRR4302542.5885989/1‑94 (MQ=57)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                              <  SRR4302542.381610/94‑1 (MQ=60)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                              <  SRR4302542.954290/94‑1 (MQ=60)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                              <  SRR4302542.1413235/94‑1 (MQ=60)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                              <  SRR4302542.2288243/94‑1 (MQ=60)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                              <  SRR4302542.3959972/94‑1 (MQ=60)
                                AGTGGCTGGTACATATATGGCCAAAGCCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                             >  SRR4302542.5168070/1‑94 (MQ=57)
                                AGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                             <  SRR4302542.6236988/94‑1 (MQ=60)
                                AGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                             <  SRR4302542.2360160/94‑1 (MQ=60)
                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                            <  SRR4302542.3703558/94‑1 (MQ=60)
                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                            >  SRR4302542.6219390/1‑94 (MQ=60)
                                    GCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATT                                         <  SRR4302542.1271349/94‑1 (MQ=60)
                                    GCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATT                                         >  SRR4302542.2636417/1‑94 (MQ=60)
                                    GCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATT                                         <  SRR4302542.1203092/94‑1 (MQ=60)
                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                        <  SRR4302542.5704804/94‑1 (MQ=60)
                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                        <  SRR4302542.5146495/94‑1 (MQ=60)
                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                        >  SRR4302542.3830192/1‑94 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                       <  SRR4302542.6086117/94‑1 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                       <  SRR4302542.6247735/94‑1 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                       >  SRR4302542.7319718/1‑94 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                       <  SRR4302542.1903768/94‑1 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                       <  SRR4302542.2459353/94‑1 (MQ=60)
                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                      <  SRR4302542.6214812/94‑1 (MQ=60)
                                       GGTACATATAGGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                      <  SRR4302542.2538183/94‑1 (MQ=60)
                                        GTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                     <  SRR4302542.1171801/94‑1 (MQ=60)
                                        GTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                     <  SRR4302542.1829843/94‑1 (MQ=60)
                                         TACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGG                                    <  SRR4302542.4106671/94‑1 (MQ=60)
                                         TACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGG                                    <  SRR4302542.3233086/94‑1 (MQ=60)
                                          ACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                   >  SRR4302542.2485962/1‑94 (MQ=60)
                                          ACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGCCATTATCGGG                                   >  SRR4302542.2012289/1‑94 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                  >  SRR4302542.5306660/1‑94 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                  <  SRR4302542.77882/94‑1 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                  <  SRR4302542.1606499/94‑1 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTAGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                  >  SRR4302542.6638846/1‑94 (MQ=60)
                                             TATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                <  SRR4302542.3244753/94‑1 (MQ=60)
                                             TATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                <  SRR4302542.2263875/94‑1 (MQ=60)
                                              ATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATG                               >  SRR4302542.3177404/1‑94 (MQ=60)
                                               TATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                              <  SRR4302542.5312681/94‑1 (MQ=60)
                                                 TGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                            >  SRR4302542.4654497/1‑94 (MQ=60)
                                                 TGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                            <  SRR4302542.5013589/94‑1 (MQ=60)
                                                 TGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                            <  SRR4302542.6395420/94‑1 (MQ=60)
                                                  GGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTA                           <  SRR4302542.7149520/94‑1 (MQ=60)
                                                  GGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTA                           <  SRR4302542.6934371/94‑1 (MQ=60)
                                                   GCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                          <  SRR4302542.2116327/94‑1 (MQ=60)
                                                   GCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                          <  SRR4302542.4212104/94‑1 (MQ=60)
                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                         >  SRR4302542.1207992/1‑94 (MQ=60)
                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                         >  SRR4302542.5589342/1‑94 (MQ=60)
                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                         <  SRR4302542.3742918/94‑1 (MQ=60)
                                                     AAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                        <  SRR4302542.4196145/94‑1 (MQ=60)
                                                     AAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                        >  SRR4302542.5848930/1‑94 (MQ=60)
                                                     AAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                        <  SRR4302542.1470004/94‑1 (MQ=60)
                                                      aaacccaggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                       <  SRR4302542.184818/73‑1 (MQ=60)
                                                      AAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                       >  SRR4302542.7252315/1‑94 (MQ=60)
                                                       AAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTA                      >  SRR4302542.3950474/1‑94 (MQ=60)
                                                       AAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTA                      <  SRR4302542.932159/94‑1 (MQ=60)
                                                       AAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTA                      <  SRR4302542.5791186/94‑1 (MQ=60)
                                                        AGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                     >  SRR4302542.6178700/1‑94 (MQ=60)
                                                        AGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                     <  SRR4302542.6974731/94‑1 (MQ=60)
                                                        AGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                     >  SRR4302542.72827/1‑94 (MQ=60)
                                                         GCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATT                    >  SRR4302542.2671587/1‑94 (MQ=60)
                                                         GCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATT                    >  SRR4302542.2761838/1‑94 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                   <  SRR4302542.6866605/94‑1 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                   <  SRR4302542.435636/94‑1 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                   <  SRR4302542.5427598/94‑1 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                   >  SRR4302542.1913623/1‑94 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                   <  SRR4302542.814505/94‑1 (MQ=60)
                                                           CCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT                  >  SRR4302542.7272473/1‑94 (MQ=60)
                                                           CCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT                  <  SRR4302542.154689/94‑1 (MQ=60)
                                                           CCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT                  >  SRR4302542.6522864/1‑94 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                 >  SRR4302542.6565189/16‑94 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                 >  SRR4302542.3767235/16‑94 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                 >  SRR4302542.5760065/16‑94 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                 <  SRR4302542.6526750/79‑1 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                <  SRR4302542.4720431/80‑1 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                >  SRR4302542.578103/15‑94 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                >  SRR4302542.4098612/15‑94 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                >  SRR4302542.4118023/15‑94 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                >  SRR4302542.6615822/15‑94 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                <  SRR4302542.4772431/80‑1 (MQ=60)
                                                              gaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAA               <  SRR4302542.5424289/81‑1 (MQ=60)
                                                               aaaacccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAT              <  SRR4302542.2524693/82‑1 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT             <  SRR4302542.7397316/83‑1 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT             <  SRR4302542.1780701/83‑1 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT             >  SRR4302542.6975919/12‑94 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT             <  SRR4302542.3601344/83‑1 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT             <  SRR4302542.5567779/83‑1 (MQ=60)
                                                                 aatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCCATTT            <  SRR4302542.7044347/84‑1 (MQ=60)
                                                                 aatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT            <  SRR4302542.5700916/84‑1 (MQ=60)
                                                                 aatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT            <  SRR4302542.3501505/84‑1 (MQ=60)
                                                                  atccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA           >  SRR4302542.4065983/10‑94 (MQ=60)
                                                                  atccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA           >  SRR4302542.5107389/10‑94 (MQ=60)
                                                                  atccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA           >  SRR4302542.4347123/10‑94 (MQ=60)
                                                                  atccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA           <  SRR4302542.5506447/85‑1 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG         >  SRR4302542.3635165/8‑94 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG         <  SRR4302542.2162817/87‑1 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG         <  SRR4302542.3487948/87‑1 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG         <  SRR4302542.5721588/87‑1 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG         >  SRR4302542.7346850/8‑94 (MQ=60)
                                                                     cgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA        <  SRR4302542.1003076/88‑1 (MQ=60)
                                                                     cgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA        <  SRR4302542.5164818/88‑1 (MQ=60)
                                                                     cgggatTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA        >  SRR4302542.7039720/7‑94 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG       >  SRR4302542.549870/6‑94 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG       <  SRR4302542.1638479/89‑1 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG       <  SRR4302542.743006/89‑1 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG       >  SRR4302542.5201029/6‑94 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG       <  SRR4302542.3664057/89‑1 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG       >  SRR4302542.2703368/6‑94 (MQ=60)
                                                                       ggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC      <  SRR4302542.1442185/90‑1 (MQ=60)
                                                                       ggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC      <  SRR4302542.2025294/90‑1 (MQ=60)
                                                                       ggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC      <  SRR4302542.1164894/90‑1 (MQ=60)
                                                                        gctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCG     <  SRR4302542.3355262/91‑1 (MQ=60)
                                                                         ctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA    <  SRR4302542.1495168/92‑1 (MQ=60)
                                                                         ctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA    >  SRR4302542.4791963/3‑94 (MQ=60)
                                                                         ctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA    <  SRR4302542.1874227/92‑1 (MQ=60)
                                                                           TCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATA  <  SRR4302542.842232/94‑1 (MQ=60)
                                                                           |                                                                                              
TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCA‑GGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATA  >  NC_000913/3957478‑3957643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑