Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,957,560 +C intergenic (‑129/‑732) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,957,5581.C97.6% 1372.8 / 15.5 411intergenic (‑127/‑734)ppiC/reppeptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand):  ref base . (9/1);  new base C (185/216);  total (194/217)
Fisher's exact test for biased strand distribution p-value = 7.69e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

TAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CC‑T‑GC‑TCGCA‑GGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957470‑3957645
                                                                                              |                                                                                       
tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGT‑cc                                                                                       >  1:3519080/1‑93 (MQ=255)
 aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CC‑GGGCTTCGCA‑GGT‑ccc                                                                                      <  2:1064051/93‑2 (MQ=255)
  gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGT‑ccct                                                                                     <  2:4730922/93‑3 (MQ=255)
  gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGT‑ccct                                                                                     >  2:181928/1‑91 (MQ=255)
       aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAgg                                                                                 <  1:5444915/93‑1 (MQ=255)
        tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCGAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAggg                                                                                >  2:1697448/1‑93 (MQ=255)
        tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAggg                                                                                <  2:1164613/93‑1 (MQ=255)
         ccGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAgggg                                                                               >  2:353311/1‑93 (MQ=255)
          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGga                                                                              <  2:4881286/93‑1 (MQ=255)
           gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGgag                                                                             >  2:4566382/1‑93 (MQ=255)
           gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGgag                                                                             >  2:6941401/1‑93 (MQ=255)
            cAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGgaga                                                                            >  2:7186010/1‑93 (MQ=255)
             aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGaa                                                                           <  2:6658278/93‑1 (MQ=255)
             aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGaa                                                                           >  2:1413406/1‑93 (MQ=255)
                tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAAc                                                                        >  2:6072275/1‑93 (MQ=255)
                tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAAc                                                                        >  1:6240871/1‑93 (MQ=255)
                 gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACt                                                                       <  1:1858189/93‑1 (MQ=255)
                   tAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTa                                                                     >  1:769790/1‑93 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAg                                                                    <  2:3930457/93‑1 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAg                                                                    <  1:7242985/93‑1 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAg                                                                    >  1:4539520/1‑93 (MQ=255)
                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAg                                                                    <  1:2142060/93‑1 (MQ=255)
                     aCGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAgg                                                                   >  1:16063/1‑93 (MQ=255)
                     aCGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAgg                                                                   >  1:1213898/1‑93 (MQ=255)
                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                                >  2:4290977/1‑93 (MQ=255)
                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                                <  2:5047279/93‑1 (MQ=255)
                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                                >  2:5215680/1‑93 (MQ=255)
                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                                <  2:1145423/93‑1 (MQ=255)
                         aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCct                                                               <  1:4167935/93‑1 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              >  2:1955670/1‑93 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              <  2:4005295/93‑1 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              <  1:4588165/93‑1 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              <  1:2804720/93‑1 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                              >  1:4908951/1‑93 (MQ=255)
                          aTTGTCAGTCAGGGAGTGGCTGGCACATATATGGCAAAAGCCCGGAAAAA‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAATGCctc                                                              >  2:6733661/1‑93 (MQ=255)
                           ttGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCctct                                                             >  1:5725900/1‑93 (MQ=255)
                            tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  2:1246648/93‑1 (MQ=255)
                            tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  2:237985/93‑1 (MQ=255)
                            tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  2:2562609/93‑1 (MQ=255)
                            tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  2:368421/93‑1 (MQ=255)
                             gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           <  1:5248299/93‑1 (MQ=255)
                             gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           <  2:2664266/93‑1 (MQ=255)
                             gtcagtcaGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           >  1:5038275/1‑93 (MQ=255)
                              tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                                          <  1:1784803/93‑1 (MQ=255)
                              tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                                          <  1:1297772/93‑1 (MQ=255)
                               cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                                         <  1:2012237/93‑1 (MQ=255)
                                 gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                       <  1:4038328/93‑1 (MQ=255)
                                  tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                      >  2:2827864/1‑93 (MQ=255)
                                  tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                      >  1:3801659/1‑93 (MQ=255)
                                  tcagGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGGCTCTATGGGCt                                                      >  1:6527962/1‑93 (MQ=255)
                                    agGGAGTGGCTGGTCCATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  2:6441802/1‑93 (MQ=255)
                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  1:3701852/1‑93 (MQ=255)
                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCCAc                                                    >  1:4709057/1‑93 (MQ=255)
                                     gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  2:3648854/1‑93 (MQ=255)
                                     gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  2:3157907/1‑93 (MQ=255)
                                     gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  1:5612325/1‑93 (MQ=255)
                                       gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCC‑G‑GCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 >  1:6648032/1‑93 (MQ=255)
                                       gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 <  1:3921276/93‑1 (MQ=255)
                                       gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 <  1:946153/93‑1 (MQ=255)
                                       gAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAA‑CCGG‑GCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 >  1:5829414/1‑93 (MQ=255)
                                        aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                                                >  2:3458358/1‑93 (MQ=255)
                                             cTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                                           <  2:6353838/93‑1 (MQ=255)
                                              tGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          <  1:2435657/93‑1 (MQ=255)
                                              tGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          <  2:5844472/93‑1 (MQ=255)
                                               ggTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  2:741027/93‑1 (MQ=255)
                                                  aCATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                      >  2:569953/1‑93 (MQ=255)
                                                  aCATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                      <  2:6223907/93‑1 (MQ=255)
                                                   cATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     <  2:6993193/93‑1 (MQ=255)
                                                     tatatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   <  1:2244573/93‑1 (MQ=255)
                                                     tatatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   <  1:3214330/93‑1 (MQ=255)
                                                       tatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                                 >  1:3147509/1‑93 (MQ=255)
                                                          ggCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt                              <  1:6866911/93‑1 (MQ=255)
                                                          ggCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt                              <  1:7080039/93‑1 (MQ=255)
                                                           gCAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                             <  2:5476560/93‑1 (MQ=255)
                                                            cAAAAGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                            <  2:5388990/93‑1 (MQ=255)
                                                             aaaaGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  1:5535530/1‑93 (MQ=255)
                                                             aaaaGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  2:4167343/1‑93 (MQ=255)
                                                             aaaaGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  2:2454424/1‑93 (MQ=255)
                                                               aaGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttt                         <  2:804487/93‑1 (MQ=255)
                                                                aGCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                        <  2:4809044/93‑1 (MQ=255)
                                                                 gCCCGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       >  1:6119491/1‑93 (MQ=255)
                                                                  cccGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      <  1:5375366/93‑1 (MQ=255)
                                                                   ccGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     <  2:2133588/93‑1 (MQ=255)
                                                                   ccGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     <  2:6880842/93‑1 (MQ=255)
                                                                   ccGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  2:6103386/1‑93 (MQ=255)
                                                                    cGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt                    <  2:6050192/93‑1 (MQ=255)
                                                                    cGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt                    >  1:6459262/1‑93 (MQ=255)
                                                                    cGGAAAAT‑CCGG‑GCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt                    <  2:3300779/93‑1 (MQ=255)
                                                                                 ggcttCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         >  1:2677529/5‑93 (MQ=255)
                                                                                 ggcttCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         >  1:5150964/5‑93 (MQ=255)
                                                                                 ggcttCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         <  1:1154996/89‑1 (MQ=255)
                                                                                  gcttCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGc        <  2:3048222/90‑1 (MQ=255)
                                                                                  gcttCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGc        >  2:7306014/4‑93 (MQ=255)
                                                                                   cttCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCg       <  1:1482368/91‑1 (MQ=255)
                                                                                     tCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     <  1:835016/93‑1 (MQ=255)
                                                                                       gCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   >  1:191796/1‑93 (MQ=255)
                                                                                       gCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   <  2:4415005/93‑1 (MQ=255)
                                                                                       gCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   <  1:2548976/93‑1 (MQ=255)
                                                                                       gCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   >  1:2301319/1‑93 (MQ=255)
                                                                                        cA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  >  1:3059417/1‑93 (MQ=255)
                                                                                              |                                                                                       
TAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CC‑T‑GC‑TCGCA‑GGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957470‑3957645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 265 total aligned reads displayed.
TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCA‑GGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  NC_000913/3957478‑3957653
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TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGCGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGG                                                                                       >  SRR4302542.2216619/1‑94 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGGGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                                     >  SRR4302542.1617432/1‑94 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                                     <  SRR4302542.4392906/94‑1 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                                     <  SRR4302542.4825409/94‑1 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                                     <  SRR4302542.864927/94‑1 (MQ=60)
  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAG                                                                                     >  SRR4302542.2594546/1‑94 (MQ=60)
     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAA                                                                                  >  SRR4302542.3934579/1‑94 (MQ=60)
     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAA                                                                                  >  SRR4302542.512056/1‑94 (MQ=60)
     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAA                                                                                  >  SRR4302542.5457962/1‑94 (MQ=60)
      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAA                                                                                 >  SRR4302542.2196350/1‑94 (MQ=60)
       ATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAAC                                                                                >  SRR4302542.4628743/1‑94 (MQ=60)
       ATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAAC                                                                                >  SRR4302542.6533165/1‑94 (MQ=60)
       ATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAAC                                                                                >  SRR4302542.6302354/1‑94 (MQ=60)
         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTT                                                                              <  SRR4302542.1874242/94‑1 (MQ=60)
         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTT                                                                              >  SRR4302542.6592303/1‑94 (MQ=60)
          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATTGCAAAAACCCGGAAAATCCGGGCCTTGCA‑GGTCCCTCAAGGGGTGAAAACTTA                                                                             >  SRR4302542.785950/1‑94 (MQ=46)
          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTA                                                                             >  SRR4302542.776450/1‑94 (MQ=60)
           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAG                                                                            <  SRR4302542.2497588/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                           <  SRR4302542.2177749/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                           <  SRR4302542.7314016/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                           <  SRR4302542.2160540/94‑1 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGG                                                                           >  SRR4302542.16203/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGG                                                                           >  SRR4302542.4588368/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                           >  SRR4302542.1224341/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                           >  SRR4302542.477544/1‑94 (MQ=60)
            AACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGG                                                                           >  SRR4302542.2278717/1‑94 (MQ=60)
             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGG                                                                          <  SRR4302542.5271031/94‑1 (MQ=60)
             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGG                                                                          <  SRR4302542.1885128/94‑1 (MQ=60)
             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGG                                                                          >  SRR4302542.1947497/1‑94 (MQ=60)
              CGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGC                                                                         >  SRR4302542.3584511/1‑94 (MQ=60)
              CGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGC                                                                         >  SRR4302542.4593176/1‑94 (MQ=60)
               GTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCC                                                                        <  SRR4302542.3155563/94‑1 (MQ=60)
               GTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCC                                                                        <  SRR4302542.3620903/94‑1 (MQ=60)
                 AATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                                      <  SRR4302542.4209041/94‑1 (MQ=60)
                 AATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                                      <  SRR4302542.3188543/94‑1 (MQ=60)
                 AATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                                      >  SRR4302542.4956697/1‑94 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                     <  SRR4302542.2831727/94‑1 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                     <  SRR4302542.557379/94‑1 (MQ=60)
                  ATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                     >  SRR4302542.5781473/1‑94 (MQ=60)
                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                   <  SRR4302542.961600/94‑1 (MQ=60)
                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                   >  SRR4302542.4691173/1‑94 (MQ=60)
                    TGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                   >  SRR4302542.5087189/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  <  SRR4302542.5299314/94‑1 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  <  SRR4302542.6503653/94‑1 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  <  SRR4302542.4962244/94‑1 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  >  SRR4302542.7093990/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  >  SRR4302542.4138927/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  >  SRR4302542.4203060/1‑94 (MQ=60)
                     GTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  >  SRR4302542.315253/1‑94 (MQ=57)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                                 <  SRR4302542.1800291/94‑1 (MQ=60)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                                 <  SRR4302542.2708145/94‑1 (MQ=60)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                                 <  SRR4302542.522764/94‑1 (MQ=60)
                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                                 <  SRR4302542.1308945/94‑1 (MQ=60)
                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                                <  SRR4302542.2029614/94‑1 (MQ=60)
                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                                <  SRR4302542.2841906/94‑1 (MQ=60)
                       CAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGG                                                                <  SRR4302542.2378480/94‑1 (MQ=60)
                        AGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGC                                                               >  SRR4302542.3625126/1‑94 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                              >  SRR4302542.3839289/1‑94 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                              <  SRR4302542.3279836/94‑1 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                              <  SRR4302542.5632951/94‑1 (MQ=60)
                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                              <  SRR4302542.4078174/94‑1 (MQ=60)
                         GTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGGCTCTATGGGCT                                                              >  SRR4302542.6592298/1‑94 (MQ=57)
                         GTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCT                                                              >  SRR4302542.6854901/1‑94 (MQ=60)
                          TCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGGCTCTATGGGCTA                                                             >  SRR4302542.3882098/1‑94 (MQ=60)
                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCTAC                                                            >  SRR4302542.838602/1‑94 (MQ=60)
                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                            >  SRR4302542.3738482/1‑94 (MQ=60)
                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCCAC                                                            >  SRR4302542.4754989/1‑94 (MQ=60)
                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                           <  SRR4302542.4754373/94‑1 (MQ=60)
                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                           <  SRR4302542.4661731/94‑1 (MQ=60)
                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                           >  SRR4302542.5666851/1‑94 (MQ=60)
                              GGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCCGGCTTCGCA‑GGTCCCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCTACAAA                                                         >  SRR4302542.6713552/1‑94 (MQ=60)
                              GGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCAGGGC‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAA                                                         >  SRR4302542.5885989/1‑94 (MQ=57)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                                        <  SRR4302542.2288243/94‑1 (MQ=60)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                                        <  SRR4302542.381610/94‑1 (MQ=60)
                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                                        <  SRR4302542.954290/94‑1 (MQ=60)
                                AGTGGCTGGTACATATATGGCCAAAGCCCGGAAAATCCGGGCTTCGCAGGGC‑CCTCAAGGGGGGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                                       >  SRR4302542.5168070/1‑94 (MQ=57)
                                AGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                                       <  SRR4302542.2360160/94‑1 (MQ=60)
                                AGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                                       <  SRR4302542.6236988/94‑1 (MQ=60)
                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                      <  SRR4302542.3703558/94‑1 (MQ=60)
                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                      >  SRR4302542.6219390/1‑94 (MQ=60)
                                    GCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATT                                                   <  SRR4302542.1203092/94‑1 (MQ=60)
                                    GCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATT                                                   <  SRR4302542.1271349/94‑1 (MQ=60)
                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                  >  SRR4302542.3830192/1‑94 (MQ=60)
                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                  <  SRR4302542.5704804/94‑1 (MQ=60)
                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                  <  SRR4302542.5146495/94‑1 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                                 >  SRR4302542.7319718/1‑94 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                                 <  SRR4302542.6086117/94‑1 (MQ=60)
                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                                 <  SRR4302542.6247735/94‑1 (MQ=60)
                                        GTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                               <  SRR4302542.1829843/94‑1 (MQ=60)
                                        GTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCG                                               <  SRR4302542.1171801/94‑1 (MQ=60)
                                         TACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGG                                              <  SRR4302542.4106671/94‑1 (MQ=60)
                                          ACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                             >  SRR4302542.2485962/1‑94 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            <  SRR4302542.77882/94‑1 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            <  SRR4302542.1606499/94‑1 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            >  SRR4302542.5306660/1‑94 (MQ=60)
                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTAGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            >  SRR4302542.6638846/1‑94 (MQ=60)
                                             TATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                          <  SRR4302542.3244753/94‑1 (MQ=60)
                                              ATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATG                                         >  SRR4302542.3177404/1‑94 (MQ=60)
                                               TATGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                                        <  SRR4302542.5312681/94‑1 (MQ=60)
                                                 TGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                                      <  SRR4302542.5013589/94‑1 (MQ=60)
                                                 TGGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                                      >  SRR4302542.4654497/1‑94 (MQ=60)
                                                  GGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTA                                     <  SRR4302542.7149520/94‑1 (MQ=60)
                                                  GGCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTA                                     <  SRR4302542.6934371/94‑1 (MQ=60)
                                                   GCAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                                    <  SRR4302542.4212104/94‑1 (MQ=60)
                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                   >  SRR4302542.1674360/1‑94 (MQ=60)
                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                   >  SRR4302542.5589342/1‑94 (MQ=60)
                                                     AAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                                  <  SRR4302542.1470004/94‑1 (MQ=60)
                                                     AAAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                                  >  SRR4302542.5848930/1‑94 (MQ=60)
                                                      AAAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                                 >  SRR4302542.7252315/1‑94 (MQ=60)
                                                       AAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTA                                <  SRR4302542.5791186/94‑1 (MQ=60)
                                                       AAGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTA                                >  SRR4302542.3950474/1‑94 (MQ=60)
                                                        AGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                               >  SRR4302542.6178700/1‑94 (MQ=60)
                                                        AGCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                               <  SRR4302542.6974731/94‑1 (MQ=60)
                                                         GCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATT                              >  SRR4302542.2761838/1‑94 (MQ=60)
                                                         GCCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATT                              >  SRR4302542.2671587/1‑94 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             <  SRR4302542.435636/94‑1 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             <  SRR4302542.5427598/94‑1 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             >  SRR4302542.1913623/1‑94 (MQ=60)
                                                          CCCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             <  SRR4302542.6866605/94‑1 (MQ=60)
                                                           CCGGAAAATCCGGGCTTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT                            >  SRR4302542.7272473/1‑94 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                           >  SRR4302542.6565189/16‑94 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                           <  SRR4302542.6526750/79‑1 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                           >  SRR4302542.3767235/16‑94 (MQ=60)
                                                            cggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                           >  SRR4302542.5760065/16‑94 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                          >  SRR4302542.4098612/15‑94 (MQ=60)
                                                             ggaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                          >  SRR4302542.578103/15‑94 (MQ=60)
                                                              gaaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAA                         <  SRR4302542.5424289/81‑1 (MQ=60)
                                                               aaaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAT                        >  SRR4302542.7243218/13‑94 (MQ=60)
                                                               aaaacccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAT                        <  SRR4302542.2524693/82‑1 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       <  SRR4302542.7397316/83‑1 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       >  SRR4302542.6975919/12‑94 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       <  SRR4302542.5567779/83‑1 (MQ=60)
                                                                aaatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       <  SRR4302542.1780701/83‑1 (MQ=60)
                                                                 aatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCCATTT                      <  SRR4302542.7044347/84‑1 (MQ=60)
                                                                 aatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT                      <  SRR4302542.4178730/84‑1 (MQ=60)
                                                                 aatccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT                      <  SRR4302542.5700916/84‑1 (MQ=60)
                                                                  atccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA                     <  SRR4302542.5506447/85‑1 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                   >  SRR4302542.977194/8‑94 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                   <  SRR4302542.5721588/87‑1 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                   >  SRR4302542.7346850/8‑94 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                   >  SRR4302542.3635165/8‑94 (MQ=60)
                                                                    ccgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                   <  SRR4302542.3487948/87‑1 (MQ=60)
                                                                     cgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA                  <  SRR4302542.1003076/88‑1 (MQ=60)
                                                                     cgggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA                  <  SRR4302542.5164818/88‑1 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 >  SRR4302542.549870/6‑94 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 <  SRR4302542.3664057/89‑1 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 >  SRR4302542.2703368/6‑94 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 <  SRR4302542.743006/89‑1 (MQ=60)
                                                                      gggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 <  SRR4302542.1638479/89‑1 (MQ=60)
                                                                       ggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC                <  SRR4302542.1442185/90‑1 (MQ=60)
                                                                       ggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC                <  SRR4302542.1164894/90‑1 (MQ=60)
                                                                       ggctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC                <  SRR4302542.2025294/90‑1 (MQ=60)
                                                                         ctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              <  SRR4302542.1495168/92‑1 (MQ=60)
                                                                         ctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              <  SRR4302542.1874227/92‑1 (MQ=60)
                                                                         ctTCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              >  SRR4302542.4791963/3‑94 (MQ=60)
                                                                           TCGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATA            <  SRR4302542.842232/94‑1 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302542.6837852/1‑94 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           <  SRR4302542.322702/94‑1 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302542.2321092/1‑94 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302542.2321091/1‑94 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302542.2153714/1‑94 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302542.193421/1‑94 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           <  SRR4302542.3961874/94‑1 (MQ=60)
                                                                            CGCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           <  SRR4302542.398220/94‑1 (MQ=60)
                                                                             GCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG          <  SRR4302542.2573672/94‑1 (MQ=60)
                                                                             GCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG          <  SRR4302542.1299925/94‑1 (MQ=60)
                                                                             GCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG          >  SRR4302542.3088595/1‑94 (MQ=60)
                                                                             GCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG          >  SRR4302542.5493240/1‑94 (MQ=60)
                                                                             GCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG          >  SRR4302542.5216246/1‑94 (MQ=60)
                                                                             GCA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG          >  SRR4302542.4004857/1‑94 (MQ=60)
                                                                              CA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         >  SRR4302542.4973956/1‑94 (MQ=60)
                                                                              CA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         <  SRR4302542.2911358/94‑1 (MQ=60)
                                                                              CA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         >  SRR4302542.7309016/1‑94 (MQ=60)
                                                                              CA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         >  SRR4302542.2272364/1‑94 (MQ=60)
                                                                              CA‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         <  SRR4302542.6378987/94‑1 (MQ=60)
                                                                               A‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        >  SRR4302542.1292869/1‑94 (MQ=60)
                                                                               A‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        >  SRR4302542.1403168/1‑94 (MQ=60)
                                                                               A‑GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        >  SRR4302542.6417142/1‑94 (MQ=60)
                                                                                 GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCA       <  SRR4302542.6618990/94‑1 (MQ=60)
                                                                                 GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCA       >  SRR4302542.3384165/1‑94 (MQ=60)
                                                                                 GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCA       <  SRR4302542.3433389/94‑1 (MQ=60)
                                                                                 GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCA       >  SRR4302542.3548397/1‑94 (MQ=60)
                                                                                  nncCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT      <  SRR4302542.3336562/91‑1 (MQ=60)
                                                                                  gtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT      >  SRR4302542.6508890/4‑94 (MQ=60)
                                                                                   tcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATT     <  SRR4302542.2083891/92‑1 (MQ=60)
                                                                                   C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG    >  SRR4302542.3582966/1‑94 (MQ=60)
                                                                                     CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   >  SRR4302542.2134705/1‑94 (MQ=60)
                                                                                     CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   <  SRR4302542.1815384/94‑1 (MQ=60)
                                                                                     CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   >  SRR4302542.6026905/1‑94 (MQ=60)
                                                                                     CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   >  SRR4302542.4572423/1‑94 (MQ=60)
                                                                                     CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   >  SRR4302542.3076502/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.6463185/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.5904406/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.4133963/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.2604378/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.4729524/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.3062735/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  <  SRR4302542.503963/94‑1 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.6590554/1‑94 (MQ=60)
                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGGCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302542.6270732/1‑94 (MQ=60)
                                                                                      |                                                                                             
TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCA‑GGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  NC_000913/3957478‑3957653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑