Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,057,859 (T)9→8 intergenic (+97/‑167) yihK → / → yihL putative GTP‑binding factor/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,057,8510T.85.4% 1052.9 / 201.1 322intergenic (+89/‑175)yihK/yihLputative GTP‑binding factor/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (21/26);  new base . (141/133);  total (163/159)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 363 total aligned reads displayed.

CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT  >  NC_000913/4057759‑4057942
                                                                                            |                                                                                           
 tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                            >  1:694444/1‑93 (MQ=255)
  gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttt                                                                                           >  1:1525720/1‑93 (MQ=255)
  gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttt                                                                                           >  2:6380415/1‑93 (MQ=255)
      gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttt                                                                                       >  1:399183/1‑93 (MQ=255)
          aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                   <  2:5944932/93‑4 (MQ=255)
              tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGt                                                                               <  2:1270062/93‑1 (MQ=255)
                  cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa                                                                          <  1:4827311/93‑1 (MQ=255)
                  cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa                                                                          <  2:5441441/93‑1 (MQ=255)
                   gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCACCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAg                                                                         >  1:3682088/1‑93 (MQ=255)
                   gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGa                                                                          <  2:623599/93‑1 (MQ=255)
                   gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAg                                                                         >  1:4693000/1‑93 (MQ=255)
                      gcgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgcgc                                                                      <  1:2611682/93‑1 (MQ=255)
                        gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCTTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcac                                                                    >  1:2997870/1‑93 (MQ=255)
                        gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcac                                                                    >  2:5211240/1‑93 (MQ=255)
                        gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcac                                                                    >  2:7281531/1‑93 (MQ=255)
                          cAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCacac                                                                  >  2:1066992/1‑93 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCacac                                                                  >  2:1852271/1‑93 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                 >  1:6890222/1‑93 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                 >  1:5458784/1‑93 (MQ=255)
                             ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                               <  2:1302009/93‑1 (MQ=255)
                              cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAt                                                              <  2:3938863/93‑1 (MQ=255)
                              cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAt                                                              <  1:7076556/93‑1 (MQ=255)
                              cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAt                                                              <  1:2075944/93‑1 (MQ=255)
                              cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTAATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAt                                                              <  1:4933441/93‑1 (MQ=255)
                               gcgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAAt                                                              <  1:561192/93‑1 (MQ=255)
                                cgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATg                                                             >  2:4146933/1‑93 (MQ=255)
                                 gcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                           >  2:969242/1‑93 (MQ=255)
                                  cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                          <  1:5752234/93‑1 (MQ=255)
                                  cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                          <  2:4507144/93‑1 (MQ=255)
                                  cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                          <  1:759310/93‑1 (MQ=255)
                                  cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                          >  1:1071600/1‑93 (MQ=255)
                                  cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                          <  1:4404435/93‑1 (MQ=255)
                                   aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                         >  1:5717837/1‑93 (MQ=255)
                                    ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGt                                                        >  1:3905902/1‑93 (MQ=255)
                                     cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                       >  1:1725437/1‑93 (MQ=255)
                                     cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                       >  1:1869700/1‑93 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                       <  1:282011/93‑1 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                      >  1:7368288/1‑93 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                      <  2:3194453/93‑1 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                      >  2:2162998/1‑93 (MQ=255)
                                       aaaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc                                                     <  1:597344/93‑1 (MQ=255)
                                       aaaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc                                                     >  2:2492850/1‑93 (MQ=255)
                                         aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtctc                                                   <  2:269872/93‑1 (MQ=255)
                                           aCGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                                 <  1:449886/93‑1 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                                 <  1:3425826/93‑1 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                                <  1:3343070/93‑1 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                                >  1:7134502/1‑93 (MQ=255)
                                             gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                               >  1:7404186/1‑93 (MQ=255)
                                              aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                              <  2:3514167/93‑1 (MQ=255)
                                              aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                              >  2:876079/1‑93 (MQ=255)
                                               ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                              >  1:5244059/1‑93 (MQ=255)
                                               ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGTCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                             <  1:3626280/93‑1 (MQ=255)
                                               ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                             <  2:689523/93‑1 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                            <  2:3492424/93‑1 (MQ=255)
                                                 aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATaa                                           >  1:5129140/1‑93 (MQ=255)
                                                 aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATaa                                           <  1:4339792/93‑1 (MQ=255)
                                                  aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                          <  2:3758875/93‑1 (MQ=255)
                                                     tCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                       <  1:1633091/93‑1 (MQ=255)
                                                      cGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                       <  2:5561415/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                     <  1:971491/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                     <  2:4765131/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                     >  1:7019792/1‑93 (MQ=255)
                                                        ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                    <  2:6635804/93‑1 (MQ=255)
                                                        ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                    <  2:6060569/93‑1 (MQ=255)
                                                         tttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTgg                                   <  2:5690403/93‑1 (MQ=255)
                                                          ttttAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                                  <  2:4058965/93‑1 (MQ=255)
                                                          ttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                                  <  2:13863/93‑1 (MQ=255)
                                                              aGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg                              <  1:1796700/93‑1 (MQ=255)
                                                               gTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg                              <  1:6264576/93‑1 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                          <  1:4302492/93‑1 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                          <  2:6480336/93‑1 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                          <  1:3183256/93‑1 (MQ=255)
                                                                   tAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                          >  2:5060805/1‑93 (MQ=255)
                                                                   tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                         <  1:6560817/93‑1 (MQ=255)
                                                                    aaaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                        <  1:2267788/93‑1 (MQ=255)
                                                                     aaaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                        <  1:6752099/93‑1 (MQ=255)
                                                                      aaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                      >  1:1223426/1‑93 (MQ=255)
                                                                      aaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                      >  2:4919049/1‑93 (MQ=255)
                                                                        aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                    >  1:6436254/1‑93 (MQ=255)
                                                                        aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                    <  2:6086158/93‑1 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                   >  1:2663040/1‑93 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                   >  1:3238652/1‑93 (MQ=255)
                                                                          ccTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                  >  2:4435464/1‑93 (MQ=255)
                                                                           cTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                  >  2:7504338/1‑93 (MQ=255)
                                                                           cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCt                 >  2:6335923/1‑93 (MQ=255)
                                                                             gCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCttt               <  2:4674233/93‑1 (MQ=255)
                                                                               cAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt             <  2:1704103/93‑1 (MQ=255)
                                                                                aGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc            >  2:4560/1‑93 (MQ=255)
                                                                                aGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc            <  2:7060184/93‑1 (MQ=255)
                                                                                 gCGATGGCAGG‑TTTTTTTTGTCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca           <  1:5375923/93‑1 (MQ=255)
                                                                                 gCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca           >  1:801112/1‑93 (MQ=255)
                                                                                  cGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcac          <  2:2813363/93‑1 (MQ=255)
                                                                                   gATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca         >  2:1768422/1‑93 (MQ=255)
                                                                                   gATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca         >  1:4089633/1‑93 (MQ=255)
                                                                                   gATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca         >  2:2614181/1‑93 (MQ=255)
                                                                                     tGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt       <  2:4301759/93‑1 (MQ=255)
                                                                                     tGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt       >  1:1029093/1‑93 (MQ=255)
                                                                                     tGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt       >  2:2885605/1‑93 (MQ=255)
                                                                                       gCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa     >  1:6062819/1‑93 (MQ=255)
                                                                                       gCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa     <  2:3564025/93‑1 (MQ=255)
                                                                                            |                                                                                           
CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT  >  NC_000913/4057759‑4057942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  NC_000913/4057766‑4057952
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AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTG                                                                                               <  SRR4302542.2949744/94‑1 (MQ=60)
 AAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTG                                                                                              <  SRR4302542.717016/94‑1 (MQ=60)
  AACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATATCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCC                                                                                            >  SRR4302542.5822180/1‑94 (MQ=60)
   ACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCT                                                                                           <  SRR4302542.3250034/94‑1 (MQ=60)
   ACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCT                                                                                           <  SRR4302542.5160642/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTG                                                                                          <  SRR4302542.7460799/94‑1 (MQ=60)
      ATCGTCGCCGCGCCACCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAAAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGT                                                                                         <  SRR4302542.1371795/94‑1 (MQ=60)
      ATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTC                                                                                        >  SRR4302542.6481320/1‑94 (MQ=60)
      ATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTC                                                                                        <  SRR4302542.523370/94‑1 (MQ=60)
       TCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGTTGGCAGG‑TTTTTTTTTGCCTGTC                                                                                        <  SRR4302542.4783191/94‑1 (MQ=60)
         GTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGT                                                                                      >  SRR4302542.514844/1‑94 (MQ=60)
         GTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTG                                                                                     >  SRR4302542.3447891/1‑94 (MQ=60)
         GTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTG                                                                                     <  SRR4302542.5505483/94‑1 (MQ=60)
         GTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTG                                                                                     >  SRR4302542.1605082/1‑94 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCACCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAG                                                                                   >  SRR4302542.3718175/1‑94 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAG                                                                                   <  SRR4302542.4874352/94‑1 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAG                                                                                   <  SRR4302542.678841/94‑1 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAG                                                                                   >  SRR4302542.4738822/1‑94 (MQ=60)
              CGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGC                                                                                >  SRR4302542.3360079/1‑94 (MQ=60)
              CGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGC                                                                                >  SRR4302542.577621/1‑94 (MQ=60)
               GCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCA                                                                               <  SRR4302542.2636928/94‑1 (MQ=60)
                CGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCTTAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCAC                                                                              >  SRR4302542.3026550/1‑94 (MQ=60)
                CGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCAC                                                                              <  SRR4302542.3498275/94‑1 (MQ=60)
                 GCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCAC                                                                              >  SRR4302542.5549738/1‑94 (MQ=60)
                 GCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACA                                                                             <  SRR4302542.7087296/94‑1 (MQ=60)
                 GCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACA                                                                             >  SRR4302542.4297210/1‑94 (MQ=60)
                  CCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACAC                                                                            <  SRR4302542.1610477/94‑1 (MQ=60)
                  CCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACAC                                                                            >  SRR4302542.484605/1‑94 (MQ=60)
                  CCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACAC                                                                            >  SRR4302542.6057006/1‑94 (MQ=60)
                   CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           >  SRR4302542.6957891/1‑94 (MQ=60)
                   CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           >  SRR4302542.5511818/1‑94 (MQ=60)
                   CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           >  SRR4302542.6957890/1‑94 (MQ=60)
                    AACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           >  SRR4302542.5937802/1‑94 (MQ=60)
                      CCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                        >  SRR4302542.7444188/1‑94 (MQ=60)
                      CCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                        <  SRR4302542.4642330/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302542.6320544/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302542.6736543/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302542.7146004/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302542.2093878/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTAATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302542.4981397/94‑1 (MQ=60)
                        GCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302542.566030/94‑1 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     <  SRR4302542.2387364/94‑1 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     <  SRR4302542.5323460/94‑1 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     >  SRR4302542.4402850/1‑94 (MQ=60)
                          GCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGA                                                                    >  SRR4302542.1080806/1‑94 (MQ=60)
                           CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   <  SRR4302542.5808075/94‑1 (MQ=60)
                           CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302542.5773335/1‑94 (MQ=60)
                           CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   <  SRR4302542.4447691/94‑1 (MQ=60)
                           CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302542.960106/1‑94 (MQ=60)
                           CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   <  SRR4302542.765890/94‑1 (MQ=60)
                            ACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302542.7054696/1‑94 (MQ=60)
                            ACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑CTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302542.2740288/1‑94 (MQ=60)
                            ACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGT                                                                  >  SRR4302542.3944461/1‑94 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                 >  SRR4302542.6500340/1‑94 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                 >  SRR4302542.5232123/1‑94 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                 >  SRR4302542.1885867/1‑94 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                 >  SRR4302542.1740414/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                 >  SRR4302542.1099154/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                >  SRR4302542.1658559/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                >  SRR4302542.7440453/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                <  SRR4302542.4368842/94‑1 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                <  SRR4302542.1986014/94‑1 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                >  SRR4302542.3764818/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                <  SRR4302542.7234766/94‑1 (MQ=60)
                               GAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                <  SRR4302542.284466/94‑1 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                              <  SRR4302542.602495/94‑1 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                              <  SRR4302542.7131636/94‑1 (MQ=60)
                                 AAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTC                                                             >  SRR4302542.700617/1‑94 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                            >  SRR4302542.2650697/1‑94 (MQ=60)
                                    ACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCT                                                          <  SRR4302542.6763393/94‑1 (MQ=60)
                                    ACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCT                                                          <  SRR4302542.453775/94‑1 (MQ=60)
                                    ACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCT                                                          >  SRR4302542.7204519/1‑94 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCT                                                          <  SRR4302542.1128230/94‑1 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCT                                                          <  SRR4302542.3457912/94‑1 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         >  SRR4302542.3970893/1‑94 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         >  SRR4302542.7476648/1‑94 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         <  SRR4302542.3374537/94‑1 (MQ=60)
                                      GATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTA                                                        >  SRR4302542.2102199/1‑94 (MQ=60)
                                      GATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTA                                                        >  SRR4302542.277684/1‑94 (MQ=60)
                                      GATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTA                                                        >  SRR4302542.5377987/1‑94 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTA                                                        >  SRR4302542.5295032/1‑94 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTA                                                        <  SRR4302542.1865877/94‑1 (MQ=60)
                                        TTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGTCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATA                                                      <  SRR4302542.3660062/94‑1 (MQ=60)
                                         TAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAA                                                     <  SRR4302542.1119041/94‑1 (MQ=60)
                                         TAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAA                                                     >  SRR4302542.5178958/1‑94 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    <  SRR4302542.4382432/94‑1 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    >  SRR4302542.1874048/1‑94 (MQ=60)
                                           ATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGT                                                   <  SRR4302542.805698/94‑1 (MQ=60)
                                           ATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGT                                                   <  SRR4302542.3083059/94‑1 (MQ=60)
                                            TTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGT                                                   <  SRR4302542.6566545/94‑1 (MQ=60)
                                            TTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTC                                                  >  SRR4302542.1388973/1‑94 (MQ=60)
                                             TTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCT                                                 <  SRR4302542.5798791/94‑1 (MQ=60)
                                              TCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTG                                                <  SRR4302542.1647176/94‑1 (MQ=60)
                                               CGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGT                                               >  SRR4302542.7088773/1‑94 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGT                                               >  SRR4302542.2557983/1‑94 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              <  SRR4302542.7388217/94‑1 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              <  SRR4302542.979860/94‑1 (MQ=60)
                                                  TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGT                                            >  SRR4302542.4505987/1‑94 (MQ=60)
                                                  TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGT                                            >  SRR4302542.1068668/1‑94 (MQ=60)
                                                  TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑‑TTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                           >  SRR4302542.829093/1‑94 (MQ=60)
                                                   TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGTCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                           <  SRR4302542.4932384/94‑1 (MQ=60)
                                                   TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                           <  SRR4302542.4932385/94‑1 (MQ=60)
                                                     TTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAC                                         >  SRR4302542.2048294/1‑94 (MQ=60)
                                                     TTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAC                                         <  SRR4302542.1610389/94‑1 (MQ=60)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACG                                        <  SRR4302542.3260158/94‑1 (MQ=60)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                       <  SRR4302542.6170727/94‑1 (MQ=60)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                       <  SRR4302542.1812283/94‑1 (MQ=60)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                       <  SRR4302542.1652912/94‑1 (MQ=60)
                                                        GTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                       <  SRR4302542.6326305/94‑1 (MQ=60)
                                                        GTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                       >  SRR4302542.5461993/1‑94 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                    >  SRR4302542.3149392/1‑94 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                    >  SRR4302542.5373625/1‑94 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                    >  SRR4302542.6946047/1‑94 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                    <  SRR4302542.6946155/94‑1 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   <  SRR4302542.4344795/94‑1 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   <  SRR4302542.1005183/94‑1 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   >  SRR4302542.2461454/1‑94 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   <  SRR4302542.4440839/94‑1 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   <  SRR4302542.3213416/94‑1 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                   >  SRR4302542.3006278/1‑94 (MQ=60)
                                                            TAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATA                                  <  SRR4302542.6625483/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302542.2287301/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 >  SRR4302542.6974701/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 >  SRR4302542.1881446/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 >  SRR4302542.7298977/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302542.4030491/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 >  SRR4302542.4690228/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302542.3689843/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGGGGTTACCTAAATAG                                 >  SRR4302542.7559865/1‑94 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302542.6818522/94‑1 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302542.807686/1‑94 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302542.6075473/1‑94 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302542.3635103/1‑94 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302542.7195703/1‑94 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302542.5575926/1‑94 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302542.1233951/1‑94 (MQ=60)
                                                               AAAGCCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATTTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                               <  SRR4302542.3457624/94‑1 (MQ=60)
                                                               AAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                               <  SRR4302542.4932646/94‑1 (MQ=60)
                                                                AAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              >  SRR4302542.6499656/1‑94 (MQ=60)
                                                                AAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              >  SRR4302542.2654526/1‑94 (MQ=60)
                                                                AAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              <  SRR4302542.1039148/94‑1 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302542.2688742/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302542.7541041/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302542.3269263/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302542.2126261/1‑94 (MQ=60)
                                                                  ACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGC                            >  SRR4302542.4158747/1‑94 (MQ=60)
                                                                  ACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGC                            >  SRR4302542.4530161/1‑94 (MQ=60)
                                                                   CCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCT                           >  SRR4302542.4075513/1‑94 (MQ=60)
                                                                   CCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCT                           >  SRR4302542.5708929/1‑94 (MQ=60)
                                                                    CTGCCGGCGAT‑GCAAG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTT                          <  SRR4302542.3680395/94‑1 (MQ=60)
                                                                    CTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCT                           <  SRR4302542.7121715/94‑1 (MQ=60)
                                                                    CTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTT                          <  SRR4302542.6358058/94‑1 (MQ=60)
                                                                    CTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTT                          >  SRR4302542.2939873/1‑94 (MQ=60)
                                                                     TGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTT                         <  SRR4302542.5745571/94‑1 (MQ=60)
                                                                     TGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTT                         <  SRR4302542.5258748/94‑1 (MQ=60)
                                                                      GCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTG                        <  SRR4302542.7573998/94‑1 (MQ=60)
                                                                       CCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGT                       <  SRR4302542.2764979/94‑1 (MQ=60)
                                                                       CCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGCGGTTACGTCAATAGAGAGCTTTGT                       <  SRR4302542.4599738/94‑1 (MQ=60)
                                                                        CAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTC                      <  SRR4302542.5546118/94‑1 (MQ=60)
                                                                        CAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTC                      >  SRR4302542.3970394/1‑94 (MQ=60)
                                                                         AGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                     >  SRR4302542.808000/1‑94 (MQ=60)
                                                                         AGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                     <  SRR4302542.1690986/94‑1 (MQ=60)
                                                                          GCGATGGCAGG‑‑TTTTTTTTGTCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCAC                    <  SRR4302542.5428163/94‑1 (MQ=60)
                                                                           CGATGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                     <  SRR4302542.6442990/94‑1 (MQ=60)
                                                                           CGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                   >  SRR4302542.4129971/1‑94 (MQ=60)
                                                                           CGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                   <  SRR4302542.5992601/94‑1 (MQ=60)
                                                                             ATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCCCACCAATGCGAGGATTTCCCTTTTAAGTCTTTGGTTACGTCAATAGAGAGCTTTGTCACAA                  >  SRR4302542.770888/1‑94 (MQ=60)
                                                                             ATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAT                 >  SRR4302542.1037959/1‑94 (MQ=60)
                                                                             ATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAT                 <  SRR4302542.3846241/94‑1 (MQ=60)
                                                                               GGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA               <  SRR4302542.3270193/94‑1 (MQ=60)
                                                                               GGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA               >  SRR4302542.6121503/1‑94 (MQ=60)
                                                                               GGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA               <  SRR4302542.4245090/94‑1 (MQ=60)
                                                                                 CAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT              <  SRR4302542.3837037/94‑1 (MQ=60)
                                                                                 CAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC             <  SRR4302542.2574280/94‑1 (MQ=60)
                                                                                  AGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302542.6962057/94‑1 (MQ=60)
                                                                                  AGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            >  SRR4302542.4918412/1‑94 (MQ=60)
                                                                                  AGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302542.7036058/94‑1 (MQ=60)
                                                                                  AGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302542.5966960/94‑1 (MQ=60)
                                                                                    G‑GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG           <  SRR4302542.7077502/94‑1 (MQ=60)
                                                                                      GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC          >  SRR4302542.5030841/1‑94 (MQ=60)
                                                                                      GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC          >  SRR4302542.5283690/1‑94 (MQ=60)
                                                                                      GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC          <  SRR4302542.4930436/94‑1 (MQ=60)
                                                                                       TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA         <  SRR4302542.4139020/94‑1 (MQ=60)
                                                                                       TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA         <  SRR4302542.2439794/94‑1 (MQ=60)
                                                                                         TTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAA       <  SRR4302542.5329540/94‑1 (MQ=60)
                                                                                         TTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAA       <  SRR4302542.4729982/94‑1 (MQ=60)
                                                                                         TTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAA       <  SRR4302542.6958689/94‑1 (MQ=60)
                                                                                          TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG      >  SRR4302542.4241114/1‑94 (MQ=60)
                                                                                          TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG      <  SRR4302542.5744341/94‑1 (MQ=60)
                                                                                              TGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  <  SRR4302542.5506063/94‑1 (MQ=60)
                                                                                              TGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  SRR4302542.4150319/1‑94 (MQ=60)
                                                                                              |                                                                                             
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  NC_000913/4057766‑4057952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑