Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,085,675 Δ1 bp intergenic (+38/+13) yiiG → / ← frvR hypothetical protein/putative frv operon regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,085,6750G.98.9% 1250.0 / ‑0.7 280intergenic (+38/+13)yiiG/frvRhypothetical protein/putative frv operon regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base . (153/123);  minor base C (0/3);  total (154/126)
Fisher's exact test for biased strand distribution p-value = 9.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 280 total aligned reads displayed.

GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085587‑4085762
                                                                                        |                                                                                       
gCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCg                                                                                    >  2:3072608/1‑93 (MQ=255)
 cATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGa                                                                                   >  1:5290418/1‑93 (MQ=255)
  aTTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGac                                                                                  >  1:5026573/1‑93 (MQ=255)
   ttACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGaca                                                                                 >  1:1069902/1‑93 (MQ=255)
    tACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGacac                                                                                >  1:4506693/1‑93 (MQ=255)
      cgcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAg                                                                              <  2:6647903/93‑1 (MQ=255)
      cgcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAg                                                                              <  2:3534660/93‑1 (MQ=255)
        cgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGtt                                                                            >  1:5737022/1‑93 (MQ=255)
         gAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTc                                                                           >  1:6025834/1‑93 (MQ=255)
          aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCa                                                                          >  1:7052980/1‑93 (MQ=255)
           gTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGGGGCGC‑ATTCGACACAGTTTAc                                                                         >  1:5899195/1‑93 (MQ=255)
           gTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAc                                                                         >  2:1010988/1‑93 (MQ=255)
           gTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAc                                                                         >  1:3783662/1‑93 (MQ=255)
             aCAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccc                                                                       <  2:1277330/93‑1 (MQ=255)
             aCAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccc                                                                       >  2:4019728/1‑93 (MQ=255)
              cAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcccc                                                                      <  1:7292772/93‑1 (MQ=255)
               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCt                                                                     >  2:3426131/1‑93 (MQ=255)
               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCt                                                                     <  1:4632105/93‑1 (MQ=255)
               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCt                                                                     <  2:6891618/93‑1 (MQ=255)
               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCt                                                                     >  2:2842456/1‑93 (MQ=255)
                aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCtt                                                                    <  2:3320122/93‑1 (MQ=255)
                aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCtt                                                                    <  1:6402460/93‑1 (MQ=255)
                aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCtt                                                                    <  1:135232/93‑1 (MQ=255)
                  gAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtgt                                                                  >  2:5375015/1‑93 (MQ=255)
                  gAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtgt                                                                  >  1:3136182/1‑93 (MQ=255)
                   aaaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTtgtg                                                                 >  2:5626122/1‑93 (MQ=255)
                    aaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGa                                                                >  1:1799059/1‑93 (MQ=255)
                     aTGGTTGATGACTATAATAGCCTGCTTAAAACTTTTTTGATGATAAATGCAGTGTGGGAGGCGGC‑CCACCCGACACAGTTCACCCCTTGTGaa                                                               <  1:2446512/93‑1 (MQ=255)
                     aTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGaa                                                               <  2:7021354/93‑1 (MQ=255)
                      tGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAg                                                              <  2:5886759/93‑1 (MQ=255)
                      tGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAg                                                              <  2:671756/93‑1 (MQ=255)
                        gTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTa                                                            >  2:394140/1‑93 (MQ=255)
                        gTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTa                                                            >  2:6819412/1‑93 (MQ=255)
                         ttGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTaa                                                           >  1:1594725/1‑93 (MQ=255)
                           gatgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACt                                                         >  2:3509261/1‑93 (MQ=255)
                           gatgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACt                                                         >  2:4634625/1‑93 (MQ=255)
                              gaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCa                                                      >  2:3866163/1‑93 (MQ=255)
                              gaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCa                                                      <  2:4778924/93‑1 (MQ=255)
                               aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCAt                                                     >  1:823928/1‑93 (MQ=255)
                               aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCAt                                                     >  2:7483022/1‑93 (MQ=255)
                                cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATa                                                    <  1:513221/93‑1 (MQ=255)
                                cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATa                                                    >  2:4578144/1‑93 (MQ=255)
                                cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATa                                                    <  2:7392489/93‑1 (MQ=255)
                                 tataATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATaa                                                   >  1:5390084/1‑93 (MQ=255)
                                 tataATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATaa                                                   >  1:154847/1‑93 (MQ=255)
                                  ataataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATCACGCG‑GATCCGACACAGTTCACCCCTTGTGAAGGAACTCCATaaa                                                  >  1:6596408/1‑93 (MQ=255)
                                    aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATg                                                >  2:1112784/1‑93 (MQ=255)
                                    aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATg                                                >  2:5052186/1‑93 (MQ=255)
                                     ataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGc                                               >  1:4244957/1‑93 (MQ=255)
                                     ataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGc                                               >  2:1389295/1‑93 (MQ=255)
                                      taGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGcc                                              <  1:124823/93‑1 (MQ=255)
                                      taGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGcc                                              <  2:1090545/93‑1 (MQ=255)
                                       aGCCTGCGTTAAAGTTTTTTGATAATAAAGGCAGTGCGGGAGGC‑GCCCCACCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCt                                             <  1:6382887/93‑1 (MQ=255)
                                        gCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTc                                            <  2:1572778/93‑1 (MQ=255)
                                        gCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTc                                            >  1:4223215/1‑93 (MQ=255)
                                        gCCTGCGTTAAACGCTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCTCCACAGCTCACCCCTTCTGAAGTAACTTCATAAATGCCtt                                            >  1:2385558/1‑92 (MQ=255)
                                         ccTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCa                                           <  1:5442155/93‑1 (MQ=255)
                                          cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCat                                          >  2:4958155/1‑93 (MQ=255)
                                           tGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCata                                         >  2:2693900/1‑93 (MQ=255)
                                           tGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCata                                         <  1:6924112/93‑1 (MQ=255)
                                              gTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATcc                                      <  1:4330808/93‑1 (MQ=255)
                                              gTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATcc                                      >  2:6508706/1‑93 (MQ=255)
                                              gTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATcc                                      >  2:6125715/1‑93 (MQ=255)
                                               ttAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCt                                     >  2:2509102/1‑93 (MQ=255)
                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    <  2:5712742/93‑1 (MQ=255)
                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    >  2:3705553/1‑93 (MQ=255)
                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    >  1:3014220/1‑93 (MQ=255)
                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    >  2:5228846/1‑93 (MQ=255)
                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    >  1:2039526/1‑93 (MQ=255)
                                                 aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGt                                   >  2:1665207/1‑93 (MQ=255)
                                                  aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  <  2:1967128/93‑1 (MQ=255)
                                                   aCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTa                                 >  1:5965360/1‑93 (MQ=255)
                                                    cGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                <  2:241588/93‑1 (MQ=255)
                                                     gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAc                               >  2:5930350/1‑93 (MQ=255)
                                                     gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAc                               <  1:6334833/93‑1 (MQ=255)
                                                     gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAc                               <  2:4197029/93‑1 (MQ=255)
                                                      tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                              <  2:815013/93‑1 (MQ=255)
                                                      tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                              >  2:6237625/1‑93 (MQ=255)
                                                      tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                              >  1:6541178/1‑93 (MQ=255)
                                                      tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                              >  1:4049845/1‑93 (MQ=255)
                                                       ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATTCCTCATATCCTGTTAAccc                             <  1:2234497/93‑1 (MQ=255)
                                                        tttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCg                            <  1:7383969/93‑1 (MQ=255)
                                                           gatgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa                         >  1:108714/1‑93 (MQ=255)
                                                           gatgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa                         <  1:283926/93‑1 (MQ=255)
                                                           gatgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa                         >  1:3361788/1‑93 (MQ=255)
                                                            atgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaa                        >  2:595702/1‑93 (MQ=255)
                                                             tgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat                       <  1:3567009/93‑1 (MQ=255)
                                                              gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      >  1:5757669/1‑93 (MQ=255)
                                                               atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa                     >  1:1660974/1‑93 (MQ=255)
                                                                tAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataat                    >  1:4848836/1‑93 (MQ=255)
                                                                   aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                 <  2:5017513/93‑1 (MQ=255)
                                                                   aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                 <  2:1518527/93‑1 (MQ=255)
                                                                    tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  1:7158384/1‑93 (MQ=255)
                                                                    tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                <  2:5536230/93‑1 (MQ=255)
                                                                          gtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGa          >  1:2745793/1‑93 (MQ=255)
                                                                              gATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt      >  2:7206986/1‑93 (MQ=255)
                                                                                tGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    <  1:477818/93‑1 (MQ=255)
                                                                                tGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    <  2:2320007/93‑1 (MQ=255)
                                                                                  cggcgC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGaca  <  1:6660629/93‑1 (MQ=255)
                                                                                  cggcgC‑ATCCGACACAGTTCACCCCTTGTAAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGaca  <  2:4161880/93‑1 (MQ=255)
                                                                                        |                                                                                       
GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085587‑4085762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCG‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  NC_000913/4085584‑4085768
                                                                                           |                                                                                              
AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATC                                                                                            >  SRR4302542.5580553/1‑94 (MQ=60)
AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATC                                                                                            >  SRR4302542.6969079/1‑94 (MQ=60)
  AGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCG                                                                                          <  SRR4302542.5206905/94‑1 (MQ=60)
   GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGA                                                                                         >  SRR4302542.4754761/1‑94 (MQ=60)
   GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGA                                                                                         >  SRR4302542.5341838/1‑94 (MQ=60)
    CATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGAC                                                                                        >  SRR4302542.5075371/1‑94 (MQ=60)
     ATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACA                                                                                       >  SRR4302542.1079096/1‑94 (MQ=60)
      TTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACAC                                                                                      >  SRR4302542.4550867/1‑94 (MQ=60)
       TACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACA                                                                                     <  SRR4302542.2510753/94‑1 (MQ=60)
        ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAG                                                                                    <  SRR4302542.5831238/94‑1 (MQ=60)
          GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTT                                                                                  <  SRR4302542.7050194/94‑1 (MQ=60)
          GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTT                                                                                  >  SRR4302542.5792725/1‑94 (MQ=60)
          GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTT                                                                                  <  SRR4302542.3843702/94‑1 (MQ=60)
          GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTT                                                                                  <  SRR4302542.4443164/94‑1 (MQ=60)
           CGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTC                                                                                 <  SRR4302542.5354595/94‑1 (MQ=60)
           CGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTC                                                                                 >  SRR4302542.6084188/1‑94 (MQ=60)
            GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCA                                                                                >  SRR4302542.6519993/1‑94 (MQ=60)
            GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCA                                                                                >  SRR4302542.7122222/1‑94 (MQ=60)
            GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCA                                                                                >  SRR4302542.2703770/1‑94 (MQ=60)
             AGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGGGGCGC‑‑ATTCGACACAGTTTAC                                                                               >  SRR4302542.5956389/1‑94 (MQ=60)
             AGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCAC                                                                               >  SRR4302542.3821092/1‑94 (MQ=60)
                ACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCC                                                                            <  SRR4302542.6141193/94‑1 (MQ=60)
                 CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCT                                                                           <  SRR4302542.7364250/94‑1 (MQ=60)
                 CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCT                                                                           >  SRR4302542.7532619/1‑94 (MQ=60)
                  AACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTT                                                                          <  SRR4302542.4677368/94‑1 (MQ=60)
                   ACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTG                                                                         <  SRR4302542.136322/94‑1 (MQ=60)
                   ACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTG                                                                         <  SRR4302542.6465543/94‑1 (MQ=60)
                    CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGT                                                                        >  SRR4302542.3472386/1‑94 (MQ=60)
                    CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGT                                                                        <  SRR4302542.4754641/94‑1 (MQ=60)
                    CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGT                                                                        >  SRR4302542.3165992/1‑94 (MQ=60)
                    CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGT                                                                        >  SRR4302542.4860044/1‑94 (MQ=60)
                    CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGT                                                                        >  SRR4302542.5485363/1‑94 (MQ=60)
                      AAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGA                                                                      >  SRR4302542.1814659/1‑94 (MQ=60)
                      AAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGA                                                                      >  SRR4302542.6098635/1‑94 (MQ=60)
                        ATGGTTGATGACTATAATAGCCTGCTTAAAACTTTTTTGATGATAAATGCAGTGTGGGAGGCGGC‑CC‑ACCCGACACAGTTCACCCCTTGTGAAG                                                                    <  SRR4302542.2470317/94‑1 (MQ=59)
                        ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAG                                                                    <  SRR4302542.4957231/94‑1 (MQ=60)
                        ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAG                                                                    <  SRR4302542.6446512/94‑1 (MQ=60)
                        ATGGTTGATGACTAAAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTTTCGGAGGCGGC‑CC‑ATCCGCCACAGTTCACCCCTTGTGAAG                                                                    <  SRR4302542.1673940/94‑1 (MQ=60)
                         TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGT                                                                   >  SRR4302542.5097175/1‑94 (MQ=60)
                         TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGT                                                                   >  SRR4302542.6030185/1‑94 (MQ=60)
                          GGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTA                                                                  <  SRR4302542.5902748/94‑1 (MQ=60)
                          GGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTA                                                                  <  SRR4302542.4277053/94‑1 (MQ=60)
                          GGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTA                                                                  <  SRR4302542.7192999/94‑1 (MQ=60)
                           GTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAA                                                                 <  SRR4302542.978235/94‑1 (MQ=60)
                           GTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAA                                                                 >  SRR4302542.1608498/1‑94 (MQ=60)
                             TGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACT                                                               <  SRR4302542.6947969/94‑1 (MQ=60)
                              GATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTC                                                              >  SRR4302542.1977626/1‑94 (MQ=60)
                                TGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCA                                                            >  SRR4302542.2231157/1‑94 (MQ=60)
                                 GACTATTAGAGCCTGTGTTAAGCGTTTTTGATGATAAATGGCGTGTCGG‑‑GCGGGGCGCACGCGGCACAGTTCACCCCTTGTGAAGTAACTCCAT                                                           <  SRR4302542.1304751/94‑1 (MQ=16)
                                 GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCAT                                                           >  SRR4302542.831046/1‑94 (MQ=60)
                                 GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCAT                                                           <  SRR4302542.6420281/94‑1 (MQ=60)
                                   CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAA                                                         >  SRR4302542.560915/1‑94 (MQ=60)
                                   CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAA                                                         <  SRR4302542.517674/94‑1 (MQ=60)
                                   CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAA                                                         >  SRR4302542.5442432/1‑94 (MQ=60)
                                   CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAA                                                         >  SRR4302542.156155/1‑94 (MQ=60)
                                    TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAA                                                        <  SRR4302542.429835/94‑1 (MQ=60)
                                    TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATCACGCG‑G‑ATCCGACACAGTTCACCCCTTGTGAAGGAACTCCATAAA                                                        >  SRR4302542.6661402/1‑94 (MQ=60)
                                      TAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATG                                                      <  SRR4302542.3735051/94‑1 (MQ=60)
                                      TAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATG                                                      >  SRR4302542.7516654/1‑94 (MQ=60)
                                       AATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGC                                                     >  SRR4302542.4286789/1‑94 (MQ=60)
                                        ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCC                                                    >  SRR4302542.6406619/1‑94 (MQ=60)
                                        ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCC                                                    >  SRR4302542.433029/1‑94 (MQ=60)
                                         TAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCT                                                   <  SRR4302542.125842/94‑1 (MQ=60)
                                          AGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTC                                                  >  SRR4302542.4264828/1‑94 (MQ=60)
                                          AGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTC                                                  >  SRR4302542.4484144/1‑94 (MQ=60)
                                          AGCCTGCGTTAAACGCTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCTCCACAGCTCACCCCTTCTGAAGTAACTTCATAAATGCCTT                                                  >  SRR4302542.2407699/1‑94 (MQ=60)
                                           GCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCA                                                 >  SRR4302542.7441380/1‑94 (MQ=60)
                                            CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302542.1850445/94‑1 (MQ=60)
                                            CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302542.5495027/94‑1 (MQ=60)
                                            CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCAT                                                >  SRR4302542.5981365/1‑94 (MQ=60)
                                            CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302542.5374303/94‑1 (MQ=60)
                                            CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302542.5286807/94‑1 (MQ=60)
                                            CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302542.1471868/94‑1 (MQ=60)
                                             CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATA                                               <  SRR4302542.4977199/94‑1 (MQ=60)
                                              TGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATAT                                              >  SRR4302542.1094846/1‑94 (MQ=60)
                                              TGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATAT                                              <  SRR4302542.6992094/94‑1 (MQ=60)
                                               GCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATC                                             <  SRR4302542.3069029/94‑1 (MQ=60)
                                                CGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCC                                            >  SRR4302542.7106919/1‑94 (MQ=60)
                                                CGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCC                                            >  SRR4302542.1798226/1‑94 (MQ=60)
                                                 GTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCT                                           <  SRR4302542.2398803/94‑1 (MQ=60)
                                                 GTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCT                                           <  SRR4302542.4373376/94‑1 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302542.3043029/1‑94 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302542.2092987/1‑94 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302542.2057163/1‑94 (MQ=60)
                                                  TAAAACGTTTTTGATGAAAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          <  SRR4302542.6770215/94‑1 (MQ=60)
                                                    AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        >  SRR4302542.7186951/1‑94 (MQ=60)
                                                    AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        >  SRR4302542.3008025/1‑94 (MQ=60)
                                                    AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        <  SRR4302542.3161807/94‑1 (MQ=60)
                                                     AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                       >  SRR4302542.6023109/1‑94 (MQ=60)
                                                     AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                       <  SRR4302542.6601097/94‑1 (MQ=60)
                                                     AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                       <  SRR4302542.1537427/94‑1 (MQ=60)
                                                       CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     >  SRR4302542.6652911/1‑94 (MQ=60)
                                                        GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                    >  SRR4302542.6605676/1‑94 (MQ=60)
                                                        GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                    >  SRR4302542.7263177/1‑94 (MQ=60)
                                                        GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                    <  SRR4302542.6397249/94‑1 (MQ=60)
                                                        GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                    >  SRR4302542.4089792/1‑94 (MQ=60)
                                                        GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                    >  SRR4302542.4669665/1‑94 (MQ=60)
                                                         TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                   >  SRR4302542.3428591/1‑94 (MQ=60)
                                                          TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATTCCTCATATCCTGTTAACCCG                                  <  SRR4302542.2253718/94‑1 (MQ=60)
                                                          TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                  <  SRR4302542.6674265/94‑1 (MQ=60)
                                                          TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                  <  SRR4302542.3362952/94‑1 (MQ=60)
                                                           TTTGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGG                                 <  SRR4302542.7456256/94‑1 (MQ=60)
                                                             TGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                               >  SRR4302542.109605/1‑94 (MQ=60)
                                                             TGATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                               >  SRR4302542.3393381/1‑94 (MQ=60)
                                                              GATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                              >  SRR4302542.4161999/1‑94 (MQ=60)
                                                              GATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                              <  SRR4302542.286398/94‑1 (MQ=60)
                                                               ATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             >  SRR4302542.5316146/1‑94 (MQ=60)
                                                               ATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             >  SRR4302542.2995875/1‑94 (MQ=60)
                                                               ATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             <  SRR4302542.3986760/94‑1 (MQ=60)
                                                               ATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             <  SRR4302542.6697891/94‑1 (MQ=60)
                                                               ATGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             >  SRR4302542.2802896/1‑94 (MQ=60)
                                                                TGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                            >  SRR4302542.1594765/1‑94 (MQ=60)
                                                                TGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                            >  SRR4302542.5813559/1‑94 (MQ=60)
                                                                TGATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                            <  SRR4302542.3600307/94‑1 (MQ=60)
                                                                 GATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                           >  SRR4302542.1675320/1‑94 (MQ=60)
                                                                  atttATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATaactctcagatcggaagagcgtcgtgtag                          >  SRR4302542.2447008/5‑65 (MQ=60)
                                                                  ATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                          >  SRR4302542.11302/1‑94 (MQ=60)
                                                                  ATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                          >  SRR4302542.1206626/1‑94 (MQ=60)
                                                                  ATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                          >  SRR4302542.4896052/1‑94 (MQ=60)
                                                                  ATAAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                          <  SRR4302542.4560521/94‑1 (MQ=60)
                                                                    AAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCT                        >  SRR4302542.1298798/1‑94 (MQ=60)
                                                                    AAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCT                        >  SRR4302542.1726227/1‑94 (MQ=60)
                                                                    AAATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCT                        >  SRR4302542.907775/1‑94 (MQ=60)
                                                                      ATGCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      >  SRR4302542.7228607/1‑94 (MQ=60)
                                                                        GCAGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGC                    >  SRR4302542.3616384/1‑94 (MQ=60)
                                                                         CAGTGTCGGATGCGGCGC‑‑AACCGGCACAGGTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCCGCAATAATCTCCGCC                   >  SRR4302542.2827752/1‑94 (MQ=60)
                                                                          AGTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                  >  SRR4302542.7387076/1‑94 (MQ=60)
                                                                           GTGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGG                 <  SRR4302542.5811293/94‑1 (MQ=60)
                                                                            TGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGA                >  SRR4302542.6446809/1‑94 (MQ=60)
                                                                            TGTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGA                >  SRR4302542.2772270/1‑94 (MQ=60)
                                                                             GTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               <  SRR4302542.2303535/94‑1 (MQ=60)
                                                                             GTCGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               >  SRR4302542.6008271/1‑94 (MQ=60)
                                                                               CGGATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGC             <  SRR4302542.1571424/94‑1 (MQ=60)
                                                                                 GATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTG           >  SRR4302542.5114892/1‑94 (MQ=60)
                                                                                 GATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTG           <  SRR4302542.565610/94‑1 (MQ=60)
                                                                                  ATGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGA          <  SRR4302542.4211972/94‑1 (MQ=60)
                                                                                   TGCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAC         <  SRR4302542.481961/94‑1 (MQ=60)
                                                                                    GCGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA        <  SRR4302542.5802946/94‑1 (MQ=60)
                                                                                     CGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACAC       <  SRR4302542.6726253/94‑1 (MQ=60)
                                                                                     CGGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACAC       >  SRR4302542.2394834/1‑94 (MQ=60)
                                                                                      GGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA      <  SRR4302542.6908029/94‑1 (MQ=60)
                                                                                      GGCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA      <  SRR4302542.213562/94‑1 (MQ=60)
                                                                                       GCGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAA     <  SRR4302542.3114992/94‑1 (MQ=60)
                                                                                        CGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAAT    <  SRR4302542.7186431/94‑1 (MQ=60)
                                                                                        CGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAAT    <  SRR4302542.6284742/94‑1 (MQ=60)
                                                                                        CGC‑‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAAT    <  SRR4302542.1700651/94‑1 (MQ=60)
                                                                                           C‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302542.1170423/94‑1 (MQ=60)
                                                                                           C‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302542.110492/94‑1 (MQ=60)
                                                                                           C‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302542.4414640/94‑1 (MQ=60)
                                                                                           |                                                                                              
AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCG‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  NC_000913/4085584‑4085768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑