Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,108,732 T→C Q119R (CAG→CGG)  tpiA ← triosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,108,7320TC84.0% 706.7 / 129.8 319Q119R (CAG→CGG) tpiAtriosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base C (137/131);  total (162/157)
Fisher's exact test for biased strand distribution p-value = 8.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 320 total aligned reads displayed.

CGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTG  >  NC_000913/4108641‑4108823
                                                                                           |                                                                                           
cgcgTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTg                                                                                            >  2:1531374/1‑93 (MQ=255)
 gcgTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGc                                                                                           >  2:6976024/1‑93 (MQ=255)
    tCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGctct                                                                                        >  2:3411365/1‑93 (MQ=255)
    tCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGctct                                                                                        >  1:3297802/1‑93 (MQ=255)
    tCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGctct                                                                                        >  2:4191975/1‑93 (MQ=255)
    tCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGctct                                                                                        <  1:6663427/93‑1 (MQ=255)
    tCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGctct                                                                                        >  2:5635094/1‑93 (MQ=255)
    tCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGctct                                                                                        >  1:2690153/1‑93 (MQ=255)
    tCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGctct                                                                                        >  2:2371687/1‑93 (MQ=255)
        tCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCa                                                                                    <  2:1797410/93‑1 (MQ=255)
        tCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCa                                                                                    <  2:7092405/93‑1 (MQ=255)
        tCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCa                                                                                    >  2:7335476/1‑93 (MQ=255)
        tCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCa                                                                                    >  1:3580759/1‑93 (MQ=255)
        tCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCa                                                                                    <  2:671749/93‑1 (MQ=255)
        tCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCa                                                                                    >  2:3299771/1‑93 (MQ=255)
        nntGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCa                                                                                    <  1:1965733/91‑1 (MQ=255)
          tGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGc                                                                                  >  2:645981/1‑93 (MQ=255)
           gACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCa                                                                                 <  2:6024998/93‑1 (MQ=255)
            aCGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCAc                                                                                >  1:3041603/1‑93 (MQ=255)
            aCGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCAc                                                                                <  1:7198266/93‑1 (MQ=255)
             cGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCAcc                                                                               <  1:7448997/93‑1 (MQ=255)
               tGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACcgc                                                                             <  1:1043233/93‑1 (MQ=255)
               tGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACcgc                                                                             >  1:4904832/1‑93 (MQ=255)
                gcgcAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACcgcg                                                                            <  2:1403880/93‑1 (MQ=255)
                  gcAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGaa                                                                          <  1:2836963/93‑1 (MQ=255)
                  gcAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGaa                                                                          >  2:3181111/1‑93 (MQ=255)
                  gcAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGaa                                                                          >  2:314959/1‑93 (MQ=255)
                  gcAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGaa                                                                          >  2:96167/1‑93 (MQ=255)
                    aaaCTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAAtt                                                                        <  2:3621988/93‑1 (MQ=255)
                     aaCTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAAttt                                                                       <  1:808336/93‑1 (MQ=255)
                     aaCTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAAttt                                                                       >  1:6983566/1‑93 (MQ=255)
                       cttcttCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAAttttt                                                                     <  2:7129366/93‑1 (MQ=255)
                       cttcttCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAAttttt                                                                     >  2:4429170/1‑93 (MQ=255)
                       cttcttCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAAttttt                                                                     >  2:2841412/1‑93 (MQ=255)
                        ttcttcAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAAtttttt                                                                    >  1:4196505/1‑93 (MQ=255)
                        ttcttcAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAAtttttt                                                                    <  2:5581846/93‑1 (MQ=255)
                         tcttcAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTc                                                                   <  2:2212421/93‑1 (MQ=255)
                         tcttcAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTc                                                                   >  1:5223897/1‑93 (MQ=255)
                           ttcAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGTACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTcgc                                                                 >  1:641761/1‑93 (MQ=255)
                           ttcAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTcgc                                                                 >  2:2494553/1‑93 (MQ=255)
                            tcAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTcgcg                                                                >  1:5940197/1‑93 (MQ=255)
                               gTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATc                                                             >  2:5895731/1‑93 (MQ=255)
                               gTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATc                                                             >  2:6296084/1‑93 (MQ=255)
                               gTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATc                                                             >  1:6271475/1‑93 (MQ=255)
                                ttttGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCa                                                            >  2:2110037/1‑93 (MQ=255)
                                ttttGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCa                                                            <  1:7351198/93‑1 (MQ=255)
                                ttttGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCa                                                            <  2:1855036/93‑1 (MQ=255)
                                 tttGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAg                                                           <  1:1555690/93‑1 (MQ=255)
                                  ttGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGt                                                          <  1:6510521/93‑1 (MQ=255)
                                     cccGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCg                                                       >  1:6174903/1‑93 (MQ=255)
                                      ccGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTACGt                                                      >  2:4891946/1‑93 (MQ=255)
                                      ccGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGt                                                      >  2:1419304/1‑93 (MQ=255)
                                       cGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTc                                                     <  1:1624723/93‑1 (MQ=255)
                                       cGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTc                                                     >  1:3962128/1‑93 (MQ=255)
                                       cGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTc                                                     <  1:863298/93‑1 (MQ=255)
                                       cGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTc                                                     <  2:5123533/93‑1 (MQ=255)
                                          ttCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGa                                                  >  1:2548104/1‑93 (MQ=255)
                                          ttCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGa                                                  >  1:4709635/1‑93 (MQ=255)
                                          ttCATTTTCAGCTTCGGTTTCACCGACGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGa                                                  <  1:236332/93‑1 (MQ=255)
                                           tCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGAt                                                 <  2:1073825/93‑1 (MQ=255)
                                             aTTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTc                                               >  1:7106511/1‑93 (MQ=255)
                                             aTTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTc                                               >  2:4733436/1‑93 (MQ=255)
                                               tttCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCtt                                             <  1:575065/93‑1 (MQ=255)
                                                ttCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCttt                                            <  1:3091309/93‑1 (MQ=255)
                                                 tCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTtg                                           >  1:6623840/1‑93 (MQ=255)
                                                   aGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTtgtg                                         >  1:4124251/1‑93 (MQ=255)
                                                    gCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTgg                                        <  1:1315244/93‑1 (MQ=255)
                                                    gCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTgg                                        <  1:705676/93‑1 (MQ=255)
                                                     cTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGt                                       <  2:384110/93‑1 (MQ=255)
                                                      ttCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTa                                      >  1:7273689/1‑93 (MQ=255)
                                                      ttCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTa                                      >  1:6837507/1‑93 (MQ=255)
                                                       tCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTaa                                     <  1:5502400/93‑1 (MQ=255)
                                                       tCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTaa                                     <  1:5388330/93‑1 (MQ=255)
                                                         ggTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGt                                   >  2:5240943/1‑93 (MQ=255)
                                                         ggTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGt                                   <  2:596491/93‑1 (MQ=255)
                                                          gTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTa                                  >  2:1274098/1‑93 (MQ=255)
                                                           tttCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTac                                 >  1:1758213/1‑93 (MQ=255)
                                                             tCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTacga                               >  2:7250335/1‑93 (MQ=255)
                                                             tCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTacga                               >  1:815387/1‑93 (MQ=255)
                                                               aCCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTacgacg                             >  1:6746685/1‑93 (MQ=255)
                                                                ccGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGt                            >  1:5154624/1‑93 (MQ=255)
                                                                 cGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGtt                           <  2:3980909/93‑1 (MQ=255)
                                                                  gATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAGTTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTc                          >  1:5658235/1‑93 (MQ=255)
                                                                   aTGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCa                         <  2:7401871/93‑1 (MQ=255)
                                                                     gCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCaga                       <  1:3830205/93‑1 (MQ=255)
                                                                     gCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCaga                       <  2:4246268/93‑1 (MQ=255)
                                                                      cacaGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCagag                      <  2:7526908/93‑1 (MQ=255)
                                                                        caGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTg                    >  2:2643878/1‑93 (MQ=255)
                                                                            aCCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCg                <  1:2898220/93‑1 (MQ=255)
                                                                              cGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCgat              <  1:3855610/93‑1 (MQ=255)
                                                                               ggAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCgatg             >  1:2181954/1‑93 (MQ=255)
                                                                                gAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCgatga            <  2:6821801/93‑1 (MQ=255)
                                                                                 aGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCgatgat           >  2:5516823/1‑93 (MQ=255)
                                                                                   tCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCgatgatga         <  2:5024817/93‑1 (MQ=255)
                                                                                    cAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCgatgatgat        <  1:4344018/93‑1 (MQ=255)
                                                                                    cAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCgatgatgat        <  2:430037/93‑1 (MQ=255)
                                                                                     aGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGatgatgatg       >  2:4041600/1‑93 (MQ=255)
                                                                                      ggCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGt      <  2:3070355/93‑1 (MQ=255)
                                                                                        ccccGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTAc    >  1:4544927/1‑93 (MQ=255)
                                                                                         ccTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACt   >  2:4623882/1‑93 (MQ=255)
                                                                                           |                                                                                           
CGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTG  >  NC_000913/4108641‑4108823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTGTG  >  NC_000913/4108639‑4108825
                                                                                             |                                                                                             
ACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCT                                                                                               <  SRR4302542.2615398/94‑1 (MQ=60)
ACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCT                                                                                               <  SRR4302542.4106411/94‑1 (MQ=60)
ACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCC                                                                                               >  SRR4302542.6969152/1‑94 (MQ=60)
ACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCC                                                                                               >  SRR4302542.5734910/1‑94 (MQ=60)
 CCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCG                                                                                              >  SRR4302542.4702813/1‑94 (MQ=60)
 CCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCG                                                                                              <  SRR4302542.3313225/94‑1 (MQ=60)
     GTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCT                                                                                          >  SRR4302542.2716091/1‑94 (MQ=60)
     GTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCT                                                                                          >  SRR4302542.3328911/1‑94 (MQ=60)
     GTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCT                                                                                          >  SRR4302542.2129054/1‑94 (MQ=60)
      TCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTT                                                                                         <  SRR4302542.6729078/94‑1 (MQ=60)
      TCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTT                                                                                         <  SRR4302542.2854223/94‑1 (MQ=60)
      TCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTT                                                                                         <  SRR4302542.5554484/94‑1 (MQ=60)
      TCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTT                                                                                         <  SRR4302542.242100/94‑1 (MQ=60)
       CGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTT                                                                                        <  SRR4302542.482605/94‑1 (MQ=60)
        GATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTC                                                                                       >  SRR4302542.7505399/1‑94 (MQ=60)
        GATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTC                                                                                       >  SRR4302542.6511422/1‑94 (MQ=60)
        GATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTC                                                                                       >  SRR4302542.5617241/1‑94 (MQ=60)
        GATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTC                                                                                       <  SRR4302542.3433412/94‑1 (MQ=60)
         ATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCA                                                                                      >  SRR4302542.3614170/1‑94 (MQ=60)
         ATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCA                                                                                      <  SRR4302542.2343621/94‑1 (MQ=60)
         ATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCA                                                                                      <  SRR4302542.3226217/94‑1 (MQ=60)
         ATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCA                                                                                      <  SRR4302542.816143/94‑1 (MQ=60)
          TCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAG                                                                                     <  SRR4302542.4037908/94‑1 (MQ=60)
          TCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAG                                                                                     <  SRR4302542.5786094/94‑1 (MQ=60)
          TCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAG                                                                                     <  SRR4302542.2617830/94‑1 (MQ=60)
          NNTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAG                                                                                     <  SRR4302542.1982717/94‑1 (MQ=60)
           CTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGC                                                                                    >  SRR4302542.2899759/1‑94 (MQ=60)
           CTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGC                                                                                    >  SRR4302542.1080482/1‑94 (MQ=60)
             GACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCAC                                                                                  >  SRR4302542.3883974/1‑94 (MQ=60)
             GACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCAC                                                                                  >  SRR4302542.3070646/1‑94 (MQ=60)
              ACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACC                                                                                 <  SRR4302542.4243926/94‑1 (MQ=60)
              ACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACC                                                                                 <  SRR4302542.7268907/94‑1 (MQ=60)
               CGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCG                                                                                <  SRR4302542.7521929/94‑1 (MQ=60)
                GTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGC                                                                               >  SRR4302542.4952540/1‑94 (MQ=60)
                 TGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCG                                                                              >  SRR4302542.5767981/1‑94 (MQ=60)
                 TGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCG                                                                              <  SRR4302542.4176219/94‑1 (MQ=60)
                 TGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCG                                                                              <  SRR4302542.6265782/94‑1 (MQ=60)
                 TGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCG                                                                              <  SRR4302542.1052187/94‑1 (MQ=60)
                  GCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGA                                                                             <  SRR4302542.844396/94‑1 (MQ=60)
                  GCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGA                                                                             >  SRR4302542.6690084/1‑94 (MQ=60)
                    GCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAAT                                                                           <  SRR4302542.2864231/94‑1 (MQ=60)
                     CAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATT                                                                          >  SRR4302542.159483/1‑94 (MQ=60)
                      AAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTT                                                                         >  SRR4302542.2291665/1‑94 (MQ=60)
                      AAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTT                                                                         >  SRR4302542.497650/1‑94 (MQ=60)
                      AAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTT                                                                         >  SRR4302542.7052156/1‑94 (MQ=60)
                      AAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTT                                                                         >  SRR4302542.2566426/1‑94 (MQ=60)
                      AAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTT                                                                         >  SRR4302542.7018828/1‑94 (MQ=60)
                       AACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTT                                                                        <  SRR4302542.815294/94‑1 (MQ=60)
                       AACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTT                                                                        <  SRR4302542.525037/94‑1 (MQ=60)
                       AACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTT                                                                        >  SRR4302542.3888947/1‑94 (MQ=60)
                        ACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTT                                                                       >  SRR4302542.593883/1‑94 (MQ=60)
                         CTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTT                                                                      >  SRR4302542.4237879/1‑94 (MQ=60)
                          TTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTC                                                                     >  SRR4302542.7600582/1‑94 (MQ=60)
                          TTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTC                                                                     >  SRR4302542.1931070/1‑94 (MQ=60)
                          TTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTC                                                                     >  SRR4302542.5274708/1‑94 (MQ=60)
                            CTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGTACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGC                                                                   >  SRR4302542.647278/1‑94 (MQ=60)
                            CTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGC                                                                   >  SRR4302542.1415921/1‑94 (MQ=60)
                            CTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGC                                                                   <  SRR4302542.6446546/94‑1 (MQ=60)
                            CTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGC                                                                   >  SRR4302542.1976806/1‑94 (MQ=60)
                             TTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCG                                                                  >  SRR4302542.5997743/1‑94 (MQ=60)
                              TCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGA                                                                 >  SRR4302542.6546767/1‑94 (MQ=60)
                               CAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGAT                                                                >  SRR4302542.6777924/1‑94 (MQ=60)
                               CAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGAT                                                                <  SRR4302542.265471/94‑1 (MQ=60)
                                AGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATC                                                               >  SRR4302542.3760808/1‑94 (MQ=60)
                                AGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATC                                                               <  SRR4302542.7153453/94‑1 (MQ=60)
                                AGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATC                                                               >  SRR4302542.6333291/1‑94 (MQ=60)
                                 GTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCA                                                              >  SRR4302542.890003/1‑94 (MQ=60)
                                 GTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCA                                                              >  SRR4302542.5584353/1‑94 (MQ=60)
                                  TTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAG                                                             <  SRR4302542.7423188/94‑1 (MQ=60)
                                  TTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAG                                                             <  SRR4302542.816416/94‑1 (MQ=60)
                                  TTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATACACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAG                                                             <  SRR4302542.6807989/94‑1 (MQ=60)
                                   TTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGT                                                            <  SRR4302542.1569115/94‑1 (MQ=60)
                                    TTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTtggcgtcaggt                                                           >  SRR4302542.2358387/1‑83 (MQ=60)
                                    TTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTT                                                           <  SRR4302542.6574690/94‑1 (MQ=60)
                                      GCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCG                                                         <  SRR4302542.3074518/94‑1 (MQ=60)
                                      GCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCG                                                         <  SRR4302542.4938082/94‑1 (MQ=60)
                                      GCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCG                                                         >  SRR4302542.6234759/1‑94 (MQ=60)
                                      GCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCG                                                         <  SRR4302542.419784/94‑1 (MQ=60)
                                       CCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGT                                                        >  SRR4302542.3441781/1‑94 (MQ=60)
                                        CCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTC                                                       <  SRR4302542.715289/94‑1 (MQ=60)
                                        CCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTC                                                       >  SRR4302542.4001250/1‑94 (MQ=60)
                                        CCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTC                                                       >  SRR4302542.6499552/1‑94 (MQ=60)
                                         CGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCA                                                      <  SRR4302542.1079732/94‑1 (MQ=60)
                                         CGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCA                                                      <  SRR4302542.1638745/94‑1 (MQ=60)
                                         CGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCA                                                      <  SRR4302542.870733/94‑1 (MQ=60)
                                          GCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTTAGGCCCCGCTCTTTCAGCACCGCGCATTTTTTCGCGATCAGTTCGTCAG                                                     >  SRR4302542.5307829/1‑94 (MQ=60)
                                          GCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAG                                                     <  SRR4302542.7318370/94‑1 (MQ=60)
                                          GCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAG                                                     >  SRR4302542.7217051/1‑94 (MQ=60)
                                           CTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGA                                                    >  SRR4302542.4755570/1‑94 (MQ=60)
                                           CTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGA                                                    <  SRR4302542.7079787/94‑1 (MQ=60)
                                           CTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGA                                                    >  SRR4302542.2572794/1‑94 (MQ=60)
                                            TTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGAT                                                   <  SRR4302542.6599620/94‑1 (MQ=60)
                                            TTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGAT                                                   >  SRR4302542.172179/1‑94 (MQ=60)
                                            TTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGAT                                                   >  SRR4302542.6722003/1‑94 (MQ=60)
                                            TTCATTTTCAGCTTCGGTTTCACCGACGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGAT                                                   <  SRR4302542.238365/94‑1 (MQ=60)
                                             TCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATT                                                  >  SRR4302542.2785069/1‑94 (MQ=60)
                                              CATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTC                                                 >  SRR4302542.7176263/1‑94 (MQ=60)
                                               ATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCT                                                >  SRR4302542.2814646/1‑94 (MQ=60)
                                                 TTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTT                                              <  SRR4302542.580005/94‑1 (MQ=60)
                                                 TTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTT                                              <  SRR4302542.7097056/94‑1 (MQ=60)
                                                  TTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTG                                             >  SRR4302542.3002843/1‑94 (MQ=60)
                                                  TTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTG                                             <  SRR4302542.3120715/94‑1 (MQ=60)
                                                  TTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTG                                             <  SRR4302542.7363713/94‑1 (MQ=60)
                                                  TTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTG                                             >  SRR4302542.6689148/1‑94 (MQ=60)
                                                  TTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTG                                             <  SRR4302542.6860449/94‑1 (MQ=60)
                                                   TCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGT                                            <  SRR4302542.2894764/94‑1 (MQ=60)
                                                    CAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTG                                           >  SRR4302542.4164878/1‑94 (MQ=60)
                                                    CAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTG                                           <  SRR4302542.6215119/94‑1 (MQ=60)
                                                    CAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTG                                           <  SRR4302542.395205/94‑1 (MQ=60)
                                                     AGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCATCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGG                                          <  SRR4302542.6272260/94‑1 (MQ=60)
                                                      GCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGT                                         <  SRR4302542.5904293/94‑1 (MQ=60)
                                                      GCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGT                                         <  SRR4302542.1326580/94‑1 (MQ=60)
                                                      GCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGT                                         <  SRR4302542.711783/94‑1 (MQ=60)
                                                       CTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTA                                        >  SRR4302542.7344983/1‑94 (MQ=60)
                                                       CTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTA                                        >  SRR4302542.6904693/1‑94 (MQ=60)
                                                        TTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAA                                       >  SRR4302542.3544612/1‑94 (MQ=60)
                                                         TCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAG                                      <  SRR4302542.5555883/94‑1 (MQ=60)
                                                         TCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAG                                      >  SRR4302542.5755935/1‑94 (MQ=60)
                                                         TCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAG                                      <  SRR4302542.5440662/94‑1 (MQ=60)
                                                          CGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGT                                     <  SRR4302542.7604391/94‑1 (MQ=60)
                                                          CGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGT                                     >  SRR4302542.7468287/1‑94 (MQ=60)
                                                          CGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGT                                     <  SRR4302542.3272641/94‑1 (MQ=60)
                                                           GGTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTA                                    >  SRR4302542.521086/1‑94 (MQ=60)
                                                            GTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTAC                                   >  SRR4302542.7418244/1‑94 (MQ=60)
                                                            GTTTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTAC                                   >  SRR4302542.1773471/1‑94 (MQ=60)
                                                              TTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGA                                 <  SRR4302542.170501/94‑1 (MQ=60)
                                                              TTCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGA                                 >  SRR4302542.822441/1‑94 (MQ=60)
                                                               TCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGAC                                <  SRR4302542.3017883/94‑1 (MQ=60)
                                                               TCACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGAC                                <  SRR4302542.3928404/94‑1 (MQ=60)
                                                                CACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACG                               >  SRR4302542.7422422/1‑94 (MQ=60)
                                                                CACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACG                               >  SRR4302542.6813074/1‑94 (MQ=60)
                                                                 ACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGT                              >  SRR4302542.5204719/1‑94 (MQ=60)
                                                                 ACCGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGT                              >  SRR4302542.5153057/1‑94 (MQ=60)
                                                                   CGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAGTTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTC                            >  SRR4302542.5713206/1‑94 (MQ=60)
                                                                   CGATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTC                            <  SRR4302542.69153/94‑1 (MQ=60)
                                                                    GATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCA                           >  SRR4302542.4416435/1‑94 (MQ=60)
                                                                    GATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCA                           <  SRR4302542.5479341/94‑1 (MQ=60)
                                                                     ATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAG                          >  SRR4302542.1602318/1‑94 (MQ=60)
                                                                     ATGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAG                          >  SRR4302542.6864664/1‑94 (MQ=60)
                                                                      TGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGA                         <  SRR4302542.1834799/94‑1 (MQ=60)
                                                                      TGCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGA                         >  SRR4302542.4417198/1‑94 (MQ=60)
                                                                       GCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAG                        <  SRR4302542.3868091/94‑1 (MQ=60)
                                                                       GCACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAG                        <  SRR4302542.3117993/94‑1 (MQ=60)
                                                                        CACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGT                       <  SRR4302542.865183/94‑1 (MQ=60)
                                                                        CACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGT                       >  SRR4302542.7319268/1‑94 (MQ=60)
                                                                        CACAGAACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGT                       <  SRR4302542.4620429/94‑1 (MQ=60)
                                                                             AACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCG                  <  SRR4302542.5156043/94‑1 (MQ=60)
                                                                              ACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGA                 <  SRR4302542.2926031/94‑1 (MQ=60)
                                                                              ACCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGA                 <  SRR4302542.6601609/94‑1 (MQ=60)
                                                                               CCGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGAT                <  SRR4302542.6436461/94‑1 (MQ=60)
                                                                                CGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATG               >  SRR4302542.1483077/1‑94 (MQ=60)
                                                                                CGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATG               <  SRR4302542.906727/94‑1 (MQ=60)
                                                                                CGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATG               <  SRR4302542.717874/94‑1 (MQ=60)
                                                                                CGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATG               >  SRR4302542.2200745/1‑94 (MQ=60)
                                                                                CGGAGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATG               <  SRR4302542.3893699/94‑1 (MQ=60)
                                                                                   AGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATG            <  SRR4302542.701868/94‑1 (MQ=60)
                                                                                   AGTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATG            <  SRR4302542.4032411/94‑1 (MQ=60)
                                                                                    GTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGA           >  SRR4302542.4344732/1‑94 (MQ=60)
                                                                                    GTCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGA           >  SRR4302542.5384450/1‑94 (MQ=60)
                                                                                     TCAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATAAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGAT          <  SRR4302542.4234710/94‑1 (MQ=60)
                                                                                      CAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATG         <  SRR4302542.6062530/94‑1 (MQ=60)
                                                                                      CAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATG         <  SRR4302542.4612136/94‑1 (MQ=60)
                                                                                      CAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATG         <  SRR4302542.4386697/94‑1 (MQ=60)
                                                                                      CAGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGANN         >  SRR4302542.3637883/1‑94 (MQ=60)
                                                                                       AGGCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGT        >  SRR4302542.2173769/1‑94 (MQ=60)
                                                                                         GCCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTAC      >  SRR4302542.4589413/1‑94 (MQ=60)
                                                                                          CCCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACT     >  SRR4302542.488429/1‑94 (MQ=60)
                                                                                           CCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTG    <  SRR4302542.6328951/94‑1 (MQ=60)
                                                                                           CCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTG    <  SRR4302542.1997952/94‑1 (MQ=60)
                                                                                           CCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTG    <  SRR4302542.2901348/94‑1 (MQ=60)
                                                                                           CCCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTG    >  SRR4302542.4599820/1‑94 (MQ=60)
                                                                                            CCGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTGT   >  SRR4302542.5951850/1‑94 (MQ=60)
                                                                                             CGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTGTG  <  SRR4302542.2004085/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCACAGAACCGGAGTCAGGCCCTGCTCTTTCAGCACCGCGAATTTTTTCGCGATCAGTTCGTCAGATTCTTTGTGGTAAGTACGACGTTCAGAGTGACCGATGATGATGTACTGTG  >  NC_000913/4108639‑4108825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑