Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,109,226 A→G L190P (CTA→CCA)  yiiQ ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,109,2260AG85.3% 719.1 / 115.6 306L190P (CTA→CCA) yiiQhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/20);  new base G (143/118);  total (168/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 308 total aligned reads displayed.

GCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAA  >  NC_000913/4109134‑4109318
                                                                                            |                                                                                            
  aGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGgc                                                                                            <  1:3572045/93‑1 (MQ=255)
    ggCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGgcgc                                                                                          >  1:3890727/1‑93 (MQ=255)
    ggCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAgcgc                                                                                          >  1:6271351/1‑93 (MQ=255)
     gcagcaGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGcc                                                                                         <  1:2606990/93‑1 (MQ=255)
      cagcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCa                                                                                        <  2:1910585/93‑1 (MQ=255)
      cagcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCa                                                                                        <  1:2304401/93‑1 (MQ=255)
       agcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAg                                                                                       >  2:3660140/1‑93 (MQ=255)
        gcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGc                                                                                      >  1:751294/1‑93 (MQ=255)
          agGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCtt                                                                                    >  2:3910331/1‑93 (MQ=255)
            gTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTaa                                                                                  <  1:3530564/93‑1 (MQ=255)
             tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAAt                                                                                 <  1:5692549/93‑1 (MQ=255)
             tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAAt                                                                                 <  1:2991590/93‑1 (MQ=255)
             tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAAt                                                                                 <  1:6719113/93‑1 (MQ=255)
              cTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATc                                                                                <  1:2763120/93‑1 (MQ=255)
               tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCg                                                                               >  1:5472471/1‑93 (MQ=255)
               tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCg                                                                               >  1:3697571/1‑93 (MQ=255)
                 tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGt                                                                             <  2:4453090/93‑1 (MQ=255)
                 tgAAACAGTATAGAGATTCATCAGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGt                                                                             <  2:1077977/93‑1 (MQ=255)
                   aaaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTc                                                                           <  2:5055244/93‑1 (MQ=255)
                     aCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCaa                                                                         <  2:2250108/93‑1 (MQ=255)
                        gTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAg                                                                      >  2:4721017/1‑93 (MQ=255)
                        gTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAg                                                                      >  2:3415296/1‑93 (MQ=255)
                           taGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGa                                                                   <  2:5745676/93‑1 (MQ=255)
                           taGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGa                                                                   >  2:183520/1‑93 (MQ=255)
                             gagaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAg                                                                 <  2:3998295/93‑1 (MQ=255)
                             gagaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAg                                                                 <  1:2206910/93‑1 (MQ=255)
                             gagaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAg                                                                 <  1:3309690/93‑1 (MQ=255)
                             gagaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAg                                                                 >  1:7182085/1‑93 (MQ=255)
                             gagaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAg                                                                 >  2:1858759/1‑93 (MQ=255)
                             gagaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAg                                                                 <  1:5425759/93‑1 (MQ=255)
                              agaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAgg                                                                <  1:2681832/93‑1 (MQ=255)
                               gaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGt                                                               <  2:7487091/93‑1 (MQ=255)
                                aTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTc                                                              <  2:6912883/93‑1 (MQ=255)
                                aTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTc                                                              >  2:2997165/1‑93 (MQ=255)
                                 ttCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCa                                                             <  1:575712/93‑1 (MQ=255)
                                   cATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGc                                                           <  2:2202096/93‑1 (MQ=255)
                                     tCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGccc                                                         <  2:7082106/93‑1 (MQ=255)
                                     tCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGccc                                                         >  2:6140156/1‑93 (MQ=255)
                                         cacaAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCttt                                                     <  1:5186044/93‑1 (MQ=255)
                                           caAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTc                                                   >  2:136031/1‑93 (MQ=255)
                                           caAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTc                                                   >  1:6540995/1‑93 (MQ=255)
                                           caAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTc                                                   >  1:1503739/1‑93 (MQ=255)
                                           caAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTc                                                   >  1:6020050/1‑93 (MQ=255)
                                            aaaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCg                                                  >  2:6501685/1‑93 (MQ=255)
                                             aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGc                                                 >  2:1123302/1‑93 (MQ=255)
                                             aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGc                                                 >  2:2518558/1‑93 (MQ=255)
                                             aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGc                                                 >  2:679934/1‑93 (MQ=255)
                                              aGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGcc                                                >  2:1790743/1‑93 (MQ=255)
                                              aGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGcc                                                >  1:383037/1‑93 (MQ=255)
                                                gctttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGt                                              <  1:4527689/93‑1 (MQ=255)
                                                gctttgctttTTGTCATTTATTCAAACCTTCAAGAGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGt                                              <  2:5291422/93‑1 (MQ=255)
                                                 ctttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGtt                                             >  2:5515004/1‑93 (MQ=255)
                                                  tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTg                                            >  2:7165626/1‑93 (MQ=255)
                                                  tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTg                                            >  1:3963854/1‑93 (MQ=255)
                                                   ttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGt                                           <  1:4682536/93‑1 (MQ=255)
                                                    tgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTc                                          <  2:6960984/93‑1 (MQ=255)
                                                     gctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTcc                                         >  1:1240328/1‑93 (MQ=255)
                                                       tttttGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCcgc                                       <  1:1654999/93‑1 (MQ=255)
                                                          ttGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGac                                    <  1:1073226/93‑1 (MQ=255)
                                                          ttGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGac                                    >  2:4155639/1‑93 (MQ=255)
                                                            gTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGacaa                                  >  2:3170993/1‑93 (MQ=255)
                                                             tCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGacaac                                 <  1:6135728/93‑1 (MQ=255)
                                                             tCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGacaac                                 >  1:2495232/1‑93 (MQ=255)
                                                              cATTTATTCAAACCTTCAAGCGATTCAGATGGCGCGAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGacaaca                                >  2:4521750/1‑93 (MQ=255)
                                                              cATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGacaaca                                >  2:5977588/1‑93 (MQ=255)
                                                              cATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGacaaca                                <  2:2443258/93‑1 (MQ=255)
                                                                tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATa                              <  2:511240/93‑1 (MQ=255)
                                                                  tATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAAc                            <  1:1182673/93‑1 (MQ=255)
                                                                    ttCAAACCTTCAAGGGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGc                          >  2:6449377/1‑93 (MQ=255)
                                                                    ttCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTTCGCGACAACATAACGc                          <  2:68435/93‑1 (MQ=255)
                                                                     tCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                         <  1:943165/93‑1 (MQ=255)
                                                                     tCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                         >  2:2693760/1‑93 (MQ=255)
                                                                     tCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                         >  2:5615076/1‑93 (MQ=255)
                                                                      cAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAg                        >  1:4131420/1‑93 (MQ=255)
                                                                       aaaCCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                       <  2:1445398/93‑1 (MQ=255)
                                                                       aaaCCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTACGCCGTTGTCCGCGACAACATAACGCAGt                       >  1:1390059/1‑93 (MQ=255)
                                                                       aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                       <  2:352/93‑1 (MQ=255)
                                                                        aaCCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                      <  2:2296863/93‑1 (MQ=255)
                                                                        aaCCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                      >  2:5370110/1‑93 (MQ=255)
                                                                          ccTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCa                    <  2:4978585/93‑1 (MQ=255)
                                                                          ccTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCa                    >  2:7238026/1‑93 (MQ=255)
                                                                           cTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAc                   <  2:6249471/93‑1 (MQ=255)
                                                                            ttCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAcc                  >  1:4889241/1‑93 (MQ=255)
                                                                             tCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCt                 >  2:3773871/1‑93 (MQ=255)
                                                                             tCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCt                 <  1:4206451/93‑1 (MQ=255)
                                                                              cAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCtt                >  2:5023585/1‑93 (MQ=255)
                                                                               aaGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc               <  1:6864497/93‑1 (MQ=255)
                                                                               aaGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc               <  1:730043/93‑1 (MQ=255)
                                                                               aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc               <  1:6928607/93‑1 (MQ=255)
                                                                                aGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCt              >  1:7412774/1‑93 (MQ=255)
                                                                                 gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTg             >  2:5188599/1‑93 (MQ=255)
                                                                                  cGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGt            <  1:5068972/93‑1 (MQ=255)
                                                                                   gATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc           <  2:1499443/93‑1 (MQ=255)
                                                                                   gATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc           >  1:2371437/1‑93 (MQ=255)
                                                                                     ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtctc         >  1:5993877/1‑93 (MQ=255)
                                                                                      tCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCg        >  1:1745902/1‑93 (MQ=255)
                                                                                       cAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCgg       <  1:7092670/93‑1 (MQ=255)
                                                                                       cAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCgg       <  2:7362319/93‑1 (MQ=255)
                                                                                          aTAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGctgt    >  1:6158765/1‑93 (MQ=255)
                                                                                           tAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGta   >  2:4321765/1‑93 (MQ=255)
                                                                                            |                                                                                            
GCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAA  >  NC_000913/4109134‑4109318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT  >  NC_000913/4109133‑4109319
                                                                                             |                                                                                             
CGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATG                                                                                               <  SRR4302542.5830144/94‑1 (MQ=60)
CGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATG                                                                                               >  SRR4302542.4585097/1‑94 (MQ=60)
CGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATG                                                                                               >  SRR4302542.279452/1‑94 (MQ=60)
 GCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGG                                                                                              <  SRR4302542.375128/94‑1 (MQ=60)
 GCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAG                                                                                              <  SRR4302542.2641086/94‑1 (MQ=60)
  CAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGC                                                                                             <  SRR4302542.6812108/94‑1 (MQ=60)
   AGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCG                                                                                            >  SRR4302542.6057021/1‑94 (MQ=60)
   AGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCG                                                                                            <  SRR4302542.3605388/94‑1 (MQ=60)
   AGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCG                                                                                            <  SRR4302542.2755699/94‑1 (MQ=60)
    GGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGC                                                                                           >  SRR4302542.3929144/1‑94 (MQ=60)
    GGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGC                                                                                           >  SRR4302542.6333163/1‑94 (MQ=60)
      GCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCA                                                                                         <  SRR4302542.2632200/94‑1 (MQ=60)
       CAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAG                                                                                        >  SRR4302542.1755193/1‑94 (MQ=60)
       CAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAG                                                                                        <  SRR4302542.2324196/94‑1 (MQ=60)
       CAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAG                                                                                        >  SRR4302542.3993297/1‑94 (MQ=60)
        AGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGC                                                                                       >  SRR4302542.757795/1‑94 (MQ=60)
         GCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCT                                                                                      <  SRR4302542.2329078/94‑1 (MQ=60)
          CAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTT                                                                                     <  SRR4302542.5007372/94‑1 (MQ=60)
           AGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTA                                                                                    >  SRR4302542.3575227/1‑94 (MQ=60)
           AGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTA                                                                                    >  SRR4302542.6055068/1‑94 (MQ=60)
            GGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAA                                                                                   >  SRR4302542.6828223/1‑94 (MQ=60)
             GTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAAT                                                                                  <  SRR4302542.3563567/94‑1 (MQ=60)
              TCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATC                                                                                 <  SRR4302542.5747823/94‑1 (MQ=60)
              TCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATC                                                                                 <  SRR4302542.5643596/94‑1 (MQ=60)
              TCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATC                                                                                 <  SRR4302542.3020219/94‑1 (MQ=60)
              TCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATC                                                                                 >  SRR4302542.7204777/1‑94 (MQ=60)
              TCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATC                                                                                 <  SRR4302542.6785284/94‑1 (MQ=60)
               CTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCG                                                                                >  SRR4302542.5525636/1‑94 (MQ=60)
               CTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCG                                                                                >  SRR4302542.3734155/1‑94 (MQ=60)
               CTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCG                                                                                <  SRR4302542.2789745/94‑1 (MQ=60)
                 GTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGT                                                                              <  SRR4302542.6571365/94‑1 (MQ=60)
                 GTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGT                                                                              >  SRR4302542.4852489/1‑94 (MQ=60)
                    AAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCA                                                                           >  SRR4302542.4193541/1‑94 (MQ=60)
                    AAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCA                                                                           <  SRR4302542.1570905/94‑1 (MQ=60)
                      ACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAAC                                                                         <  SRR4302542.4511903/94‑1 (MQ=60)
                       CAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACTTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACA                                                                        >  SRR4302542.4760051/1‑94 (MQ=60)
                        AGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAG                                                                       <  SRR4302542.5505871/94‑1 (MQ=60)
                         GTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGC                                                                      >  SRR4302542.2498386/1‑94 (MQ=60)
                          TATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCG                                                                     <  SRR4302542.830669/94‑1 (MQ=60)
                           ATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGA                                                                    >  SRR4302542.5940864/1‑94 (MQ=60)
                             AGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAG                                                                  <  SRR4302542.5781152/94‑1 (MQ=60)
                             AGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAG                                                                  >  SRR4302542.7252518/1‑94 (MQ=60)
                              GAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGG                                                                 <  SRR4302542.5478441/94‑1 (MQ=60)
                              GAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGG                                                                 <  SRR4302542.3340894/94‑1 (MQ=60)
                              GAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGG                                                                 <  SRR4302542.2225898/94‑1 (MQ=60)
                               AGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGT                                                                <  SRR4302542.2583800/94‑1 (MQ=60)
                               AGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGT                                                                >  SRR4302542.5513825/1‑94 (MQ=60)
                               AGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGT                                                                >  SRR4302542.3638857/1‑94 (MQ=60)
                               AGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGT                                                                <  SRR4302542.2707704/94‑1 (MQ=60)
                                 ATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCA                                                              <  SRR4302542.2680195/94‑1 (MQ=60)
                                  TTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAG                                                             <  SRR4302542.580659/94‑1 (MQ=60)
                                  TTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAG                                                             <  SRR4302542.7396685/94‑1 (MQ=60)
                                  TTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAG                                                             >  SRR4302542.7151599/1‑94 (MQ=60)
                                   TCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGC                                                            <  SRR4302542.5228064/94‑1 (MQ=60)
                                        GGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTT                                                       <  SRR4302542.7506783/94‑1 (MQ=60)
                                         GCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTT                                                      <  SRR4302542.1250979/94‑1 (MQ=60)
                                          CACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT                                                     <  SRR4302542.5236425/94‑1 (MQ=60)
                                          CACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT                                                     >  SRR4302542.7178559/1‑94 (MQ=60)
                                          CACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT                                                     <  SRR4302542.258078/94‑1 (MQ=60)
                                           ACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTC                                                    >  SRR4302542.1516744/1‑94 (MQ=60)
                                           ACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTC                                                    >  SRR4302542.6078354/1‑94 (MQ=60)
                                           ACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTC                                                    >  SRR4302542.6605489/1‑94 (MQ=60)
                                           ACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTC                                                    <  SRR4302542.6988588/94‑1 (MQ=60)
                                            CAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCG                                                   >  SRR4302542.5951292/1‑94 (MQ=60)
                                            CAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCG                                                   <  SRR4302542.4651348/94‑1 (MQ=60)
                                            CAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCG                                                   <  SRR4302542.7113448/94‑1 (MQ=60)
                                             AAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGC                                                  >  SRR4302542.2999152/1‑94 (MQ=60)
                                             AAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGC                                                  >  SRR4302542.3892811/1‑94 (MQ=60)
                                             AAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGC                                                  >  SRR4302542.4167096/1‑94 (MQ=60)
                                              AAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCC                                                 >  SRR4302542.386365/1‑94 (MQ=60)
                                               AGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCG                                                >  SRR4302542.5683937/1‑94 (MQ=60)
                                               AGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCG                                                >  SRR4302542.2752673/1‑94 (MQ=60)
                                               AGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCG                                                <  SRR4302542.7461523/94‑1 (MQ=60)
                                                GGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGT                                               >  SRR4302542.141115/1‑94 (MQ=60)
                                                GGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGT                                               <  SRR4302542.6296119/94‑1 (MQ=60)
                                                 GCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTT                                              <  SRR4302542.4572038/94‑1 (MQ=60)
                                                 GCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTT                                              <  SRR4302542.493557/94‑1 (MQ=60)
                                                  CTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTG                                             <  SRR4302542.2168269/94‑1 (MQ=60)
                                                  CTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTG                                             >  SRR4302542.6186533/1‑94 (MQ=60)
                                                  CTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTG                                             >  SRR4302542.1641578/1‑94 (MQ=60)
                                                  CTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTG                                             >  SRR4302542.4002993/1‑94 (MQ=60)
                                                   TTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT                                            >  SRR4302542.4688921/1‑94 (MQ=60)
                                                    TTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTC                                           <  SRR4302542.4728289/94‑1 (MQ=60)
                                                    TTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTC                                           >  SRR4302542.5261700/1‑94 (MQ=60)
                                                     TGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCC                                          >  SRR4302542.1250986/1‑94 (MQ=60)
                                                       CTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGcttgaaggtttgaataaatgacaaaaagcaaagcctttgt                                        <  SRR4302542.5331728/94‑41 (MQ=60)
                                                        TTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG                                       <  SRR4302542.1669296/94‑1 (MQ=60)
                                                         TTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGA                                      <  SRR4302542.3796224/94‑1 (MQ=60)
                                                          TTTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGAC                                     <  SRR4302542.7539717/94‑1 (MQ=60)
                                                           TTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACA                                    <  SRR4302542.1082449/94‑1 (MQ=60)
                                                           TTGTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACA                                    >  SRR4302542.1267938/1‑94 (MQ=60)
                                                            TGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAA                                   >  SRR4302542.1286001/1‑94 (MQ=60)
                                                            GGTCATTTTTTCAAACCTTCAAGCGATTCGGATAGCGCCAGCTTATTCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCGGTTGTCCCCGACAA                                   >  SRR4302542.3726439/1‑94 (MQ=60)
                                                             GTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAAC                                  >  SRR4302542.3194619/1‑94 (MQ=60)
                                                             GTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAAC                                  >  SRR4302542.2519432/1‑94 (MQ=60)
                                                             GTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAAC                                  >  SRR4302542.1701589/1‑94 (MQ=60)
                                                             GTCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAAC                                  <  SRR4302542.6675550/94‑1 (MQ=60)
                                                              TCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACA                                 <  SRR4302542.6195091/94‑1 (MQ=60)
                                                              TCATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACA                                 >  SRR4302542.6517674/1‑94 (MQ=60)
                                                              TCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACA                                 <  SRR4302542.2312377/94‑1 (MQ=60)
                                                                ATTTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATA                               >  SRR4302542.2351160/1‑94 (MQ=60)
                                                                ATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATA                               <  SRR4302542.3605335/94‑1 (MQ=60)
                                                                 TTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAA                              <  SRR4302542.3883487/94‑1 (MQ=60)
                                                                  TTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAAC                             <  SRR4302542.4129249/94‑1 (MQ=60)
                                                                  TTATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAAC                             <  SRR4302542.4221890/94‑1 (MQ=60)
                                                                   TATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACG                            <  SRR4302542.1192877/94‑1 (MQ=60)
                                                                   TATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACG                            >  SRR4302542.3408880/1‑94 (MQ=60)
                                                                    ATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGC                           >  SRR4302542.6255419/1‑94 (MQ=60)
                                                                    ATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGC                           <  SRR4302542.2711496/94‑1 (MQ=60)
                                                                    ATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGC                           >  SRR4302542.5457736/1‑94 (MQ=60)
                                                                    ATTCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGC                           >  SRR4302542.4471727/1‑94 (MQ=60)
                                                                      TCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAG                         <  SRR4302542.951282/94‑1 (MQ=60)
                                                                      TCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAG                         >  SRR4302542.4172127/1‑94 (MQ=60)
                                                                      TCAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAG                         <  SRR4302542.6787446/94‑1 (MQ=60)
                                                                       CAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGT                        <  SRR4302542.7601428/94‑1 (MQ=60)
                                                                       CAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGT                        >  SRR4302542.6162611/1‑94 (MQ=60)
                                                                       CAAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTACGCCGTTGTCCGCGACAACATAACGCAGT                        >  SRR4302542.1402060/1‑94 (MQ=60)
                                                                        AAACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTG                       <  SRR4302542.3304763/94‑1 (MQ=60)
                                                                         AACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGC                      >  SRR4302542.5118915/1‑94 (MQ=60)
                                                                          ACCTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCA                     >  SRR4302542.5755324/1‑94 (MQ=60)
                                                                            CTTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACC                   >  SRR4302542.4936801/1‑94 (MQ=60)
                                                                            CTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACC                   >  SRR4302542.7295468/1‑94 (MQ=60)
                                                                             TTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCT                  <  SRR4302542.2477351/94‑1 (MQ=60)
                                                                             TTCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCT                  >  SRR4302542.3997340/1‑94 (MQ=60)
                                                                              TCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTT                 <  SRR4302542.6565312/94‑1 (MQ=60)
                                                                              TCAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTT                 <  SRR4302542.4247922/94‑1 (MQ=60)
                                                                              TCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTT                 <  SRR4302542.667523/94‑1 (MQ=60)
                                                                               CAAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTC                <  SRR4302542.6996483/94‑1 (MQ=60)
                                                                               CAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTC                >  SRR4302542.1143038/1‑94 (MQ=60)
                                                                                AAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCT               <  SRR4302542.6931938/94‑1 (MQ=60)
                                                                                AAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCT               >  SRR4302542.6211684/1‑94 (MQ=60)
                                                                                AAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCT               <  SRR4302542.1515297/94‑1 (MQ=60)
                                                                                AAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCT               >  SRR4302542.7485321/1‑94 (MQ=60)
                                                                                AAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCT               <  SRR4302542.7272109/94‑1 (MQ=60)
                                                                                AAGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCT               <  SRR4302542.736340/94‑1 (MQ=60)
                                                                                AAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCT               <  SRR4302542.6996634/94‑1 (MQ=60)
                                                                                 AGCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTG              >  SRR4302542.120115/1‑94 (MQ=60)
                                                                                  GCGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGT             <  SRR4302542.2945030/94‑1 (MQ=60)
                                                                                   CGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTC            <  SRR4302542.5118231/94‑1 (MQ=60)
                                                                                   CGATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTC            >  SRR4302542.2392288/1‑94 (MQ=60)
                                                                                    GATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCT           >  SRR4302542.1169043/1‑94 (MQ=60)
                                                                                    GATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCT           <  SRR4302542.4609454/94‑1 (MQ=60)
                                                                                     ATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTC          >  SRR4302542.97096/1‑94 (MQ=60)
                                                                                     ATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTC          >  SRR4302542.3341567/1‑94 (MQ=60)
                                                                                     ATTCAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTC          >  SRR4302542.3801992/1‑94 (MQ=60)
                                                                                     ATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTC          >  SRR4302542.6051932/1‑94 (MQ=60)
                                                                                      TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCG         >  SRR4302542.1761051/1‑94 (MQ=60)
                                                                                        CAGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGT       <  SRR4302542.7162250/94‑1 (MQ=60)
                                                                                         AGATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTG      >  SRR4302542.2228053/1‑94 (MQ=60)
                                                                                          GATGGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGT     >  SRR4302542.790134/1‑94 (MQ=60)
                                                                                          GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGT     >  SRR4302542.6218419/1‑94 (MQ=60)
                                                                                          GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGCTGT     >  SRR4302542.6218420/1‑94 (MQ=60)
                                                                                            TAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAA   >  SRR4302542.1941182/1‑94 (MQ=60)
                                                                                             GGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT  >  SRR4302542.3511589/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAAT  >  NC_000913/4109133‑4109319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑