Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,224,188 C→T R928C (CGT→TGT)  metH → B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,224,1880CT85.6% 743.7 / 78.8 278R928C (CGT→TGT) metHB12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand):  ref base C (25/15);  new base T (114/124);  total (139/139)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 278 total aligned reads displayed.

GAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCG  >  NC_000913/4224097‑4224278
                                                                                           |                                                                                          
gAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCg                                                                                           >  2:2087340/1‑93 (MQ=255)
 aaaCCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGt                                                                                          <  2:2649721/93‑1 (MQ=255)
  aaCCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGtc                                                                                         <  2:5975578/93‑1 (MQ=255)
  aaCCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGtc                                                                                         >  1:5457208/1‑93 (MQ=255)
  aaCCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGtc                                                                                         >  2:1356510/1‑93 (MQ=255)
   aCCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGtct                                                                                        <  2:2929186/93‑1 (MQ=255)
   aCCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGtct                                                                                        >  2:2567670/1‑93 (MQ=255)
    ccGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGtctc                                                                                       <  2:7454526/93‑1 (MQ=255)
     cgcgCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCg                                                                                      >  2:7334554/1‑93 (MQ=255)
     cgcgCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCg                                                                                      <  1:4592133/93‑1 (MQ=255)
       cgcACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGc                                                                                    <  1:587452/93‑1 (MQ=255)
       cgcACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGc                                                                                    >  2:5173406/1‑93 (MQ=255)
        gcACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCg                                                                                   >  2:5235804/1‑93 (MQ=255)
           caccacCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGc                                                                                >  2:4678623/1‑93 (MQ=255)
           caccacCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGc                                                                                >  2:3479571/1‑93 (MQ=255)
            accaccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCa                                                                               <  1:7428808/93‑1 (MQ=255)
             ccaccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAg                                                                              <  1:6167622/93‑1 (MQ=255)
              caccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAgg                                                                             >  2:6647492/1‑93 (MQ=255)
              caccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAgg                                                                             <  1:4888548/93‑1 (MQ=255)
               accGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGa                                                                            >  2:589991/1‑93 (MQ=255)
                 cGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAg                                                                          >  1:745238/1‑93 (MQ=255)
                 cGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAg                                                                          <  2:2406167/93‑1 (MQ=255)
                  ggTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGt                                                                         >  2:4211827/1‑93 (MQ=255)
                   gTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTc                                                                        <  1:3300289/93‑1 (MQ=255)
                    tCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCg                                                                       >  2:1796303/1‑93 (MQ=255)
                     cACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGa                                                                      <  1:5050244/93‑1 (MQ=255)
                        gCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGc                                                                   <  1:6790933/93‑1 (MQ=255)
                        gCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGc                                                                   >  2:3989372/1‑93 (MQ=255)
                        gCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGc                                                                   >  1:6678493/1‑93 (MQ=255)
                        gCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGc                                                                   >  1:3009801/1‑93 (MQ=255)
                        gCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCCGTGGCGCACCGTCTCCGCGCGCAGGAAGTCGAAGc                                                                   >  1:5275223/1‑93 (MQ=255)
                         cTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGGCGAAGcc                                                                  >  1:4563147/1‑93 (MQ=255)
                          tGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCa                                                                 <  1:5642949/93‑1 (MQ=255)
                          tGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCa                                                                 >  1:6319302/1‑93 (MQ=255)
                             aaGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCa                                                              <  2:7191068/93‑1 (MQ=255)
                               gcggcgCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCGGGAAGTCGAAGCCAGCATc                                                            >  1:7523869/1‑93 (MQ=255)
                               gcggcgCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATc                                                            <  1:431195/93‑1 (MQ=255)
                               gcggcgCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATc                                                            >  2:6519795/1‑93 (MQ=255)
                                cggcgCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCg                                                           <  2:4732720/93‑1 (MQ=255)
                                 ggcgCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGa                                                          >  1:3504329/1‑93 (MQ=255)
                                 ggcgCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGa                                                          <  1:5346556/93‑1 (MQ=255)
                                   cgcgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGaaa                                                        <  2:4999215/93‑1 (MQ=255)
                                   cgcgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGaaa                                                        <  1:357370/93‑1 (MQ=255)
                                   cgcgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGaaa                                                        <  2:4412825/93‑1 (MQ=255)
                                    gcgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAAc                                                       <  2:1037579/93‑1 (MQ=255)
                                    gcgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAAc                                                       >  2:1509797/1‑93 (MQ=255)
                                        gATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTg                                                   >  1:7486965/1‑93 (MQ=255)
                                        gATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTg                                                   >  1:2627310/1‑93 (MQ=255)
                                         aTAACGATTTCGCTTTTGACTGGTAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGc                                                  <  2:6263632/93‑1 (MQ=255)
                                          tAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCg                                                 >  2:6960480/1‑93 (MQ=255)
                                          tAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCg                                                 >  1:7159734/1‑93 (MQ=255)
                                          tAACGATTTCGCTTTTGACTGGCAGGCTTACACCCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGGCGAAGCCAGCATCGAAACGCTGCg                                                 >  1:2028314/1‑93 (MQ=255)
                                           aaCGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGt                                                >  2:4041969/1‑93 (MQ=255)
                                            aCGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTa                                               >  1:1318963/1‑93 (MQ=255)
                                            aCGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTa                                               <  2:5550211/93‑1 (MQ=255)
                                            aCGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTa                                               <  1:949661/93‑1 (MQ=255)
                                             cGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTaa                                              <  1:7068849/93‑1 (MQ=255)
                                              gATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAAt                                             >  2:3916026/1‑93 (MQ=255)
                                              gATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAAt                                             >  1:3880455/1‑93 (MQ=255)
                                                tttCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTa                                           >  2:7034456/1‑93 (MQ=255)
                                                  tCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACa                                         >  1:1603510/1‑93 (MQ=255)
                                                    gCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATc                                       >  1:288359/1‑93 (MQ=255)
                                                    gCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATc                                       >  2:1643433/1‑93 (MQ=255)
                                                    gCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATc                                       <  2:6158143/93‑1 (MQ=255)
                                                    gCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATc                                       <  2:3177292/93‑1 (MQ=255)
                                                      ttttGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGa                                     >  1:6379943/1‑93 (MQ=255)
                                                      ttttGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGa                                     <  1:4843846/93‑1 (MQ=255)
                                                      ttttGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGa                                     >  2:191187/1‑93 (MQ=255)
                                                      ttttGACTGGCAGGCTTACACGCCGCCGGGGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGGAATTACATCGa                                     >  1:2750008/1‑93 (MQ=255)
                                                        ttGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACt                                   <  2:452259/93‑1 (MQ=255)
                                                         tGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTg                                  >  1:7306206/1‑93 (MQ=255)
                                                            cTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGac                               >  1:5690607/1‑93 (MQ=255)
                                                            cTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGac                               >  1:3738437/1‑93 (MQ=255)
                                                                  ggCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGtt                         >  1:2111375/1‑93 (MQ=255)
                                                                  ggCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGtt                         >  1:6878037/1‑93 (MQ=255)
                                                                  ggCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGtt                         >  2:1200252/1‑93 (MQ=255)
                                                                   gCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTc                        <  1:6430405/93‑1 (MQ=255)
                                                                    cTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCt                       >  2:4272221/1‑93 (MQ=255)
                                                                    cTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCt                       <  2:3782430/93‑1 (MQ=255)
                                                                     ttACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCtt                      >  1:2324787/1‑93 (MQ=255)
                                                                     ttACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCtt                      <  1:1109512/93‑1 (MQ=255)
                                                                       acacGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTa                    <  1:5232703/93‑1 (MQ=255)
                                                                       acacGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTa                    <  2:7111427/93‑1 (MQ=255)
                                                                       acacGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTa                    <  1:2712460/93‑1 (MQ=255)
                                                                        cacGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAt                   >  1:5998744/1‑93 (MQ=255)
                                                                         acGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATg                  >  2:5811093/1‑93 (MQ=255)
                                                                           gccgccGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGAc                >  1:6985809/1‑93 (MQ=255)
                                                                           gccgccGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGAc                >  1:4120767/1‑93 (MQ=255)
                                                                            ccgccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGAcc               >  1:3486039/1‑93 (MQ=255)
                                                                             cgccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCt              <  1:4705438/93‑1 (MQ=255)
                                                                             cgccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCt              <  1:5114621/93‑1 (MQ=255)
                                                                              gccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTg             <  1:1917978/93‑1 (MQ=255)
                                                                              gccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTg             <  2:3580549/93‑1 (MQ=255)
                                                                              gccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTg             >  1:3098937/1‑93 (MQ=255)
                                                                               ccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTgg            >  2:6020325/1‑93 (MQ=255)
                                                                               ccgGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTgg            >  1:6932972/1‑93 (MQ=255)
                                                                                    ggCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCt       <  1:5580245/93‑1 (MQ=255)
                                                                                       gcACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGc    <  1:2475191/93‑1 (MQ=255)
                                                                                        cACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGcc   >  2:4945972/1‑93 (MQ=255)
                                                                                         aCCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCg  >  2:4561822/1‑93 (MQ=255)
                                                                                           |                                                                                          
GAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCG  >  NC_000913/4224097‑4224278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGA  >  NC_000913/4224095‑4224281
                                                                                             |                                                                                             
AAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACT                                                                                               >  SRR4302542.367154/1‑94 (MQ=60)
 AGAGACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACGCGCCGCCGGTGGCGCACTG                                                                                              <  SRR4302542.3919886/94‑1 (MQ=60)
 AGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTG                                                                                              <  SRR4302542.6920626/94‑1 (MQ=60)
  GAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGT                                                                                             >  SRR4302542.4371076/1‑94 (MQ=60)
   AAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTC                                                                                            >  SRR4302542.5510219/1‑94 (MQ=60)
   AAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTC                                                                                            >  SRR4302542.4812739/1‑94 (MQ=60)
      CCGCGCACACCGCCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACCCGCCGCCGGTGGCGCACTGTCTCG                                                                                         <  SRR4302542.2050987/94‑1 (MQ=60)
       CGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGG                                                                                        <  SRR4302542.4637044/94‑1 (MQ=60)
       CGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGG                                                                                        <  SRR4302542.5866988/94‑1 (MQ=60)
        GCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCGGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGC                                                                                       <  SRR4302542.5669321/94‑1 (MQ=60)
         CGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCG                                                                                      <  SRR4302542.592519/94‑1 (MQ=60)
          GCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGG                                                                                     >  SRR4302542.7593275/1‑94 (MQ=60)
           CACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTG                                                                                    <  SRR4302542.5691456/94‑1 (MQ=60)
           CACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTG                                                                                    >  SRR4302542.4224941/1‑94 (MQ=60)
           CACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTG                                                                                    <  SRR4302542.676184/94‑1 (MQ=60)
            ACACGACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGC                                                                                   >  SRR4302542.7039256/1‑94 (MQ=60)
             CACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCA                                                                                  <  SRR4302542.3163858/94‑1 (MQ=60)
              ACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAG                                                                                 <  SRR4302542.7501506/94‑1 (MQ=60)
               CCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGG                                                                                <  SRR4302542.6227378/94‑1 (MQ=60)
                CACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGA                                                                               <  SRR4302542.7548508/94‑1 (MQ=60)
                CACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGA                                                                               <  SRR4302542.4936102/94‑1 (MQ=60)
                  CCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAG                                                                             >  SRR4302542.751691/1‑94 (MQ=60)
                  CCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAG                                                                             <  SRR4302542.7319153/94‑1 (MQ=60)
                    GGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCCCTGTCTCGGCGTGCAGGAAGGC                                                                           >  SRR4302542.2480708/1‑94 (MQ=60)
                    GGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTC                                                                           <  SRR4302542.2788761/94‑1 (MQ=60)
                     GTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCG                                                                          <  SRR4302542.7267126/94‑1 (MQ=60)
                     GTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGCGCAGGAAGTCG                                                                          <  SRR4302542.3593971/94‑1 (MQ=60)
                     GTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCG                                                                          <  SRR4302542.3331423/94‑1 (MQ=60)
                       CACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAA                                                                        <  SRR4302542.5099274/94‑1 (MQ=60)
                         CGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGC                                                                      <  SRR4302542.6760838/94‑1 (MQ=60)
                         CGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGC                                                                      >  SRR4302542.6744292/1‑94 (MQ=60)
                         CGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGC                                                                      >  SRR4302542.6494932/1‑94 (MQ=60)
                         CGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGC                                                                      >  SRR4302542.3038580/1‑94 (MQ=60)
                         CGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCCGTGGCGCACCGTCTCCGCGCGCAGGAAGTCGAAGC                                                                      >  SRR4302542.5326485/1‑94 (MQ=60)
                          GCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCC                                                                     <  SRR4302542.6857718/94‑1 (MQ=60)
                          GCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGGCGAAGCC                                                                     >  SRR4302542.4607820/1‑94 (MQ=60)
                          GCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGGCGAAGCC                                                                     >  SRR4302542.5513068/1‑94 (MQ=60)
                           CTGGAAGCGGCGCGCGATACCGATTTCGCTTTTGACTGGCAGGCTTACCCCCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCA                                                                    <  SRR4302542.7548938/94‑1 (MQ=60)
                           CTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCA                                                                    >  SRR4302542.6381584/1‑94 (MQ=60)
                           CTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCA                                                                    <  SRR4302542.5099963/94‑1 (MQ=60)
                            TGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAG                                                                   <  SRR4302542.5697744/94‑1 (MQ=60)
                              GAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCA                                                                 <  SRR4302542.1885007/94‑1 (MQ=60)
                              GAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCA                                                                 <  SRR4302542.3809797/94‑1 (MQ=60)
                              GAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCA                                                                 <  SRR4302542.3048641/94‑1 (MQ=60)
                               AAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCAT                                                                >  SRR4302542.7189623/1‑94 (MQ=60)
                               AAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCAT                                                                >  SRR4302542.4702163/1‑94 (MQ=60)
                               AAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCAT                                                                >  SRR4302542.3124841/1‑94 (MQ=60)
                               AAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCAT                                                                <  SRR4302542.5861593/94‑1 (MQ=60)
                                AGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCGGGAAGTCGAAGCCAGCATC                                                               >  SRR4302542.7597850/1‑94 (MQ=60)
                                 GCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCG                                                              <  SRR4302542.434927/94‑1 (MQ=60)
                                 GCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCG                                                              <  SRR4302542.6926955/94‑1 (MQ=60)
                                  CGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGA                                                             >  SRR4302542.3537109/1‑94 (MQ=60)
                                   GGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAA                                                            <  SRR4302542.2972389/94‑1 (MQ=60)
                                   GGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAA                                                            <  SRR4302542.5398488/94‑1 (MQ=60)
                                   GGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAA                                                            <  SRR4302542.6472841/94‑1 (MQ=60)
                                     CGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAAC                                                          <  SRR4302542.7585515/94‑1 (MQ=60)
                                     CGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAAC                                                          <  SRR4302542.360487/94‑1 (MQ=60)
                                      GCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACG                                                         <  SRR4302542.4926378/94‑1 (MQ=60)
                                      GCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACG                                                         >  SRR4302542.7411803/1‑94 (MQ=60)
                                        GCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCT                                                       <  SRR4302542.3515732/94‑1 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGGTTTAACACCGCCGGTGGCGCACTTTCTCGGCGTGCAGGGAGTCGcaagcagcacctaaaagatg                                                      >  SRR4302542.1702943/1‑74 (MQ=39)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGggggccgggaagccaaacccacctcgaaacgctc                                                      >  SRR4302542.6298405/1‑60 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTG                                                      >  SRR4302542.6414498/1‑94 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTG                                                      >  SRR4302542.1332043/1‑94 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTG                                                      >  SRR4302542.3536747/1‑94 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTG                                                      >  SRR4302542.7560385/1‑94 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTG                                                      <  SRR4302542.4186547/94‑1 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTG                                                      >  SRR4302542.2652692/1‑94 (MQ=60)
                                         CGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTG                                                      >  SRR4302542.5524804/1‑94 (MQ=60)
                                          GATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGC                                                     >  SRR4302542.3524667/1‑94 (MQ=60)
                                          GATAACGATTTCGCTTTTGACTGGCAGGCTTACAAGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGC                                                     >  SRR4302542.2330867/1‑94 (MQ=60)
                                           ATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGGAGTCGAAGCCAGCAACGAAAAGCTGCG                                                    >  SRR4302542.2544595/1‑94 (MQ=60)
                                           ATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCG                                                    <  SRR4302542.628553/94‑1 (MQ=60)
                                           ATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCG                                                    <  SRR4302542.2957339/94‑1 (MQ=60)
                                           ATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCG                                                    >  SRR4302542.357509/1‑94 (MQ=60)
                                           ATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCG                                                    >  SRR4302542.7229965/1‑94 (MQ=60)
                                           ATAACGATTTCGCTTTTGACTGGCAGGCTTACACCCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGGCGAAGCCAGCATCGAAACGCTGCG                                                    >  SRR4302542.2045855/1‑94 (MQ=60)
                                             AACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTA                                                  >  SRR4302542.1330326/1‑94 (MQ=60)
                                             AACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTA                                                  >  SRR4302542.2193009/1‑94 (MQ=60)
                                              ACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAA                                                 <  SRR4302542.957819/94‑1 (MQ=60)
                                               CGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAAT                                                <  SRR4302542.7138231/94‑1 (MQ=60)
                                               CGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAAT                                                >  SRR4302542.3918784/1‑94 (MQ=60)
                                                GATTTCGCTTTTGACTGGCAGGCTTACCCGCCGCCGGGGGGGCCCTGGCTCGGCGTGGAGGAAGTCGAAGCCAGgcttgaaaccgcgggtaatt                                               <  SRR4302542.5853351/94‑21 (MQ=39)
                                                  TTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTAC                                             <  SRR4302542.2848540/94‑1 (MQ=60)
                                                  TTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTAC                                             <  SRR4302542.137860/94‑1 (MQ=60)
                                                  TTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGGAATTAC                                             >  SRR4302542.519318/1‑94 (MQ=60)
                                                   TTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACA                                            >  SRR4302542.1617360/1‑94 (MQ=60)
                                                   TTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACA                                            <  SRR4302542.945738/94‑1 (MQ=60)
                                                   TTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACA                                            >  SRR4302542.3928933/1‑94 (MQ=60)
                                                    TCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACAT                                           <  SRR4302542.1003491/94‑1 (MQ=60)
                                                    TCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACAT                                           <  SRR4302542.2493135/94‑1 (MQ=60)
                                                    TCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACAT                                           <  SRR4302542.2666966/94‑1 (MQ=60)
                                                     CGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATC                                          <  SRR4302542.4966262/94‑1 (MQ=60)
                                                     CGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATC                                          <  SRR4302542.6390180/94‑1 (MQ=60)
                                                     CGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATC                                          >  SRR4302542.290874/1‑94 (MQ=60)
                                                     CGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATC                                          >  SRR4302542.2768139/1‑94 (MQ=60)
                                                       CTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGA                                        >  SRR4302542.4665277/1‑94 (MQ=60)
                                                       CTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGA                                        >  SRR4302542.6442756/1‑94 (MQ=60)
                                                       CTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGA                                        >  SRR4302542.4474700/1‑94 (MQ=60)
                                                       CTTTTGACTGGCAGGCTTACACGCCGCCGGGGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGA                                        >  SRR4302542.6983080/1‑94 (MQ=60)
                                                       CTTTTGACTGGCAGGCTTACACGCCGCCGGGGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGGAATTACATCGA                                        >  SRR4302542.2776516/1‑94 (MQ=60)
                                                        TTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGAC                                       <  SRR4302542.1291706/94‑1 (MQ=60)
                                                        TTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGAC                                       <  SRR4302542.4891014/94‑1 (MQ=60)
                                                         TTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACT                                      <  SRR4302542.6981746/94‑1 (MQ=60)
                                                         TTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACT                                      <  SRR4302542.5728942/94‑1 (MQ=60)
                                                          TTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTG                                     >  SRR4302542.7377775/1‑94 (MQ=60)
                                                          TTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTG                                     <  SRR4302542.5294455/94‑1 (MQ=60)
                                                           TGACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGG                                    >  SRR4302542.4819732/1‑94 (MQ=60)
                                                             ACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGAC                                  >  SRR4302542.7407671/1‑94 (MQ=60)
                                                             ACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGAC                                  >  SRR4302542.3775440/1‑94 (MQ=60)
                                                             ACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGAC                                  >  SRR4302542.5745871/1‑94 (MQ=60)
                                                             ACTGGCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGAC                                  <  SRR4302542.5377526/94‑1 (MQ=60)
                                                                 GCAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCG                              <  SRR4302542.3650811/94‑1 (MQ=60)
                                                                  CAGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGT                             <  SRR4302542.365323/94‑1 (MQ=60)
                                                                   AGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTT                            >  SRR4302542.6945597/1‑94 (MQ=60)
                                                                   AGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTT                            >  SRR4302542.4979828/1‑94 (MQ=60)
                                                                   AGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTT                            >  SRR4302542.2129598/1‑94 (MQ=60)
                                                                   AGGCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTT                            >  SRR4302542.6142843/1‑94 (MQ=60)
                                                                     GCTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCT                          <  SRR4302542.6493760/94‑1 (MQ=60)
                                                                      CTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTT                         >  SRR4302542.2344717/1‑94 (MQ=60)
                                                                      CTTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTT                         >  SRR4302542.6698662/1‑94 (MQ=60)
                                                                       TTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTT                        <  SRR4302542.7068847/94‑1 (MQ=60)
                                                                       TTACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTT                        <  SRR4302542.1119005/94‑1 (MQ=60)
                                                                       TTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTT                        <  SRR4302542.4892808/94‑1 (MQ=60)
                                                                         ACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAT                      <  SRR4302542.2731002/94‑1 (MQ=60)
                                                                         ACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAT                      <  SRR4302542.2738622/94‑1 (MQ=60)
                                                                         ACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAT                      <  SRR4302542.4042674/94‑1 (MQ=60)
                                                                         ACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAT                      <  SRR4302542.5283584/94‑1 (MQ=60)
                                                                         ACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAT                      >  SRR4302542.6056842/1‑94 (MQ=60)
                                                                         ACACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTAT                      <  SRR4302542.7107706/94‑1 (MQ=60)
                                                                          CACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATG                     >  SRR4302542.4632364/1‑94 (MQ=60)
                                                                           ACGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGA                    <  SRR4302542.2010989/94‑1 (MQ=60)
                                                                            CGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGAC                   >  SRR4302542.714672/1‑94 (MQ=60)
                                                                            CGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGAC                   >  SRR4302542.4161369/1‑94 (MQ=60)
                                                                            CGCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGAC                   >  SRR4302542.7054425/1‑94 (MQ=60)
                                                                             GCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACC                  >  SRR4302542.873451/1‑94 (MQ=60)
                                                                             GCCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACC                  >  SRR4302542.3518648/1‑94 (MQ=60)
                                                                              CCGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCT                 >  SRR4302542.2639573/1‑94 (MQ=60)
                                                                               CGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTG                >  SRR4302542.6028242/1‑94 (MQ=60)
                                                                               CGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTG                <  SRR4302542.4751344/94‑1 (MQ=60)
                                                                               CGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTG                <  SRR4302542.5164300/94‑1 (MQ=60)
                                                                               CGCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTG                >  SRR4302542.3128392/1‑94 (MQ=60)
                                                                                GCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGG               <  SRR4302542.7524653/94‑1 (MQ=60)
                                                                                GCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGG               >  SRR4302542.7001042/1‑94 (MQ=60)
                                                                                GCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGG               <  SRR4302542.1934550/94‑1 (MQ=60)
                                                                                GCCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGG               <  SRR4302542.185153/94‑1 (MQ=60)
                                                                                GCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGG               <  SRR4302542.5290368/94‑1 (MQ=60)
                                                                                 CCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGT              >  SRR4302542.5394755/1‑94 (MQ=60)
                                                                                 CCGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGT              <  SRR4302542.3453011/94‑1 (MQ=60)
                                                                                  CGGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTC             >  SRR4302542.6105398/1‑94 (MQ=60)
                                                                                   GGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCG            >  SRR4302542.5704947/1‑94 (MQ=60)
                                                                                   GGTGGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCG            <  SRR4302542.5498926/94‑1 (MQ=60)
                                                                                      GGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTG         >  SRR4302542.6944384/1‑94 (MQ=60)
                                                                                      GGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTG         <  SRR4302542.1944798/94‑1 (MQ=60)
                                                                                      GGCGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTG         <  SRR4302542.5634468/94‑1 (MQ=60)
                                                                                        CGCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGC       <  SRR4302542.5218232/94‑1 (MQ=60)
                                                                                         GCACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCC      <  SRR4302542.2499201/94‑1 (MQ=60)
                                                                                          CACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCG     >  SRR4302542.5291041/1‑94 (MQ=60)
                                                                                          CACTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCG     >  SRR4302542.6601222/1‑94 (MQ=60)
                                                                                            CTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGG   >  SRR4302542.710046/1‑94 (MQ=60)
                                                                                            CTGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGG   >  SRR4302542.4214440/1‑94 (MQ=60)
                                                                                             TGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGA  >  SRR4302542.225457/1‑94 (MQ=60)
                                                                                             TGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGA  >  SRR4302542.1140533/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGA  >  NC_000913/4224095‑4224281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑