Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,228,096	A→G	N55S (AAC→AGC)	yjbC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,228,096	0	A	G	86.8%	741.3 / 99.9	302	N55S (AAC→AGC)	yjbC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (21/19); new base G (139/123); total (160/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

Only 100 of 303 total aligned reads displayed.

GGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATG  >  NC_000913/4228004‑4228185

  aTCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACg                                                                                         <  2:3943901/93‑1 (MQ=255)

   tCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCgg                                                                                        <  2:5674594/93‑1 (MQ=255)

   tCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCgg                                                                                        >  2:6269600/1‑93 (MQ=255)

   tCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCgg                                                                                        >  2:7054964/1‑93 (MQ=255)

    cGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGt                                                                                       >  1:4686580/1‑93 (MQ=255)

     gCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTc                                                                                      >  1:333930/1‑93 (MQ=255)

      cTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCa                                                                                     >  1:1753291/1‑93 (MQ=255)

      cTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCa                                                                                     <  2:2232427/93‑1 (MQ=255)

       tataTCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAg                                                                                    >  2:5218570/1‑93 (MQ=255)

       tataTCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAg                                                                                    >  2:3779840/1‑93 (MQ=255)

       tataTCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAg                                                                                    >  2:1900728/1‑93 (MQ=255)

        atatCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGt                                                                                   >  2:4393891/1‑93 (MQ=255)

         tatCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGtt                                                                                  >  2:7389720/1‑93 (MQ=255)

         tatCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGtt                                                                                  >  1:6915906/1‑93 (MQ=255)

          atCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGttg                                                                                 >  1:3896063/1‑93 (MQ=255)

           tCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGttga                                                                                <  2:5058029/93‑1 (MQ=255)

            cGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGttgat                                                                               <  2:1815/93‑1 (MQ=255)

            cGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGttgat                                                                               <  1:6102688/93‑1 (MQ=255)

             gAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGttgatt                                                                              >  2:230481/1‑93 (MQ=255)

             gAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGttgatt                                                                              <  2:2626753/93‑1 (MQ=255)

              aGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGttgattg                                                                             <  1:6219767/93‑1 (MQ=255)

                 aaGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAAc                                                                          >  1:6737963/1‑93 (MQ=255)

                  aGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAAcc                                                                         >  1:56997/1‑93 (MQ=255)

                   ggCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCt                                                                        >  2:2107134/1‑93 (MQ=255)

                     cAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCg                                                                      <  1:1242242/93‑1 (MQ=255)

                     cAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCg                                                                      >  1:1456048/1‑93 (MQ=255)

                     cAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCg                                                                      <  1:5229913/93‑1 (MQ=255)

                      aaTGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCgg                                                                     <  2:5515930/93‑1 (MQ=255)

                       aTGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCggg                                                                    >  2:6757074/1‑93 (MQ=255)

                       aTGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCggg                                                                    <  2:3379280/93‑1 (MQ=255)

                        tgtgTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGa                                                                   >  2:2942560/1‑93 (MQ=255)

                        tgtgTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGa                                                                   >  2:6826144/1‑93 (MQ=255)

                         gtgtTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGaa                                                                  <  2:2811506/93‑1 (MQ=255)

                          tgtTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAg                                                                 >  1:3805573/1‑93 (MQ=255)

                            ttCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGcc                                                               >  1:3097762/1‑93 (MQ=255)

                            ttCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGcc                                                               <  1:2286417/93‑1 (MQ=255)

                              ccTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGa                                                             >  1:3293381/1‑93 (MQ=255)

                               cTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGaa                                                            >  1:5049999/1‑93 (MQ=255)

                                ttAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAg                                                           <  2:4484045/93‑1 (MQ=255)

                                ttAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAg                                                           >  1:4573982/1‑93 (MQ=255)

                                ttAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAg                                                           >  2:1217068/1‑93 (MQ=255)

                                 tAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGa                                                          >  1:3674417/1‑93 (MQ=255)

                                 tAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGa                                                          <  2:4763482/93‑1 (MQ=255)

                                   aTGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGAtt                                                        >  1:4807926/1‑93 (MQ=255)

                                    tGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGAttt                                                       >  2:1092080/1‑93 (MQ=255)

                                       cAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGt                                                    >  1:6060156/1‑93 (MQ=255)

                                        aaGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTa                                                   <  1:2260030/93‑1 (MQ=255)

                                        aaGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTa                                                   >  1:7373867/1‑93 (MQ=255)

                                          gCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACt                                                 <  2:6105632/93‑1 (MQ=255)

                                            gAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACttg                                               <  1:4867499/93‑2 (MQ=255)

                                             aGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTAt                                              <  1:2060693/93‑1 (MQ=255)

                                             aGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTAt                                              <  2:2573672/93‑1 (MQ=255)

                                              gCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATc                                             >  2:1772248/1‑93 (MQ=255)

                                              gCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATc                                             >  1:876335/1‑93 (MQ=255)

                                              gCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATc                                             <  2:7184506/93‑1 (MQ=255)

                                               ccaccaTTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCg                                            >  2:6529972/1‑93 (MQ=255)

                                                 accaTTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGcc                                          >  1:1070692/1‑93 (MQ=255)

                                                   caTTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCt                                        >  2:1518244/1‑93 (MQ=255)

                                                         cGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACaa                                  <  1:3518612/93‑1 (MQ=255)

                                                         cGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACaa                                  <  2:6667177/93‑1 (MQ=255)

                                                          gATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAg                                 <  1:4301335/93‑1 (MQ=255)

                                                           aTCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGc                                >  2:148757/1‑93 (MQ=255)

                                                           aTCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGc                                >  1:5297473/1‑93 (MQ=255)

                                                           aTCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGc                                >  1:819486/1‑93 (MQ=255)

                                                           aTCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGc                                >  1:5825537/1‑93 (MQ=255)

                                                            tCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGcc                               <  2:5332745/93‑1 (MQ=255)

                                                            tCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGcc                               >  1:2354757/1‑93 (MQ=255)

                                                             cAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGccc                              <  1:832438/93‑1 (MQ=255)

                                                              aGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCg                             <  2:7487501/93‑1 (MQ=255)

                                                              aGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCg                             >  2:4952467/1‑93 (MQ=255)

                                                               ggTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGt                            <  1:397742/93‑1 (MQ=255)

                                                               ggTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGt                            >  2:2406655/1‑93 (MQ=255)

                                                                gTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGtt                           >  2:4031273/1‑93 (MQ=255)

                                                                  gAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTgg                         >  2:6034603/1‑93 (MQ=255)

                                                                  gAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTgg                         <  1:5649375/93‑1 (MQ=255)

                                                                  gAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTgg                         >  1:2856970/1‑93 (MQ=255)

                                                                   aaaCCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGt                        >  2:6393460/1‑93 (MQ=255)

                                                                    aaCCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTa                       <  2:4303995/93‑1 (MQ=255)

                                                                     aCCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTAt                      >  1:1613642/1‑93 (MQ=255)

                                                                     aCCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTAt                      >  1:6397336/1‑93 (MQ=255)

                                                                      cccGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTAtt                     >  2:5923240/1‑93 (MQ=255)

                                                                       ccGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTg                    >  2:2495498/1‑93 (MQ=255)

                                                                       ccGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTg                    <  1:4338264/93‑1 (MQ=255)

                                                                         ggCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTa                  <  1:6004331/93‑1 (MQ=255)

                                                                         ggCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTa                  <  2:4162567/93‑1 (MQ=255)

                                                                          gCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTaa                 <  1:7069213/93‑1 (MQ=255)

                                                                            gACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGc               >  2:4920666/1‑93 (MQ=255)

                                                                              cGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcac             <  2:272488/93‑1 (MQ=255)

                                                                                ttGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcacca           <  2:2059007/93‑1 (MQ=255)

                                                                                 tgtgAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcaccac          <  2:1574564/93‑1 (MQ=255)

                                                                                 tgtgAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGAACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGcaccac          >  1:2971637/1‑93 (MQ=255)

                                                                                  gtgAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCaccacc         >  2:5865966/1‑93 (MQ=255)

                                                                                   tgAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCg        <  1:2946620/93‑1 (MQ=255)

                                                                                    gAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGa       <  1:4633581/93‑1 (MQ=255)

                                                                                    gAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGa       >  1:4141497/1‑93 (MQ=255)

                                                                                    gAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGa       >  2:4698133/1‑93 (MQ=255)

                                                                                       aGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGa    <  2:5401998/93‑1 (MQ=255)

                                                                                        gTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGAt   <  2:3559824/93‑1 (MQ=255)

                                                                                        gTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGAt   <  1:2665180/93‑1 (MQ=255)

                                                                                         tAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATg  >  2:1423380/1‑93 (MQ=255)

GGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATG  >  NC_000913/4228004‑4228185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCG  >  NC_000913/4228003‑4228188

CGGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAG                                                                                              >  SRR4302542.2323821/1‑94 (MQ=60)

CGGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAG                                                                                              >  SRR4302542.7326081/1‑94 (MQ=60)

CGGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAG                                                                                              >  SRR4302542.1453450/1‑94 (MQ=60)

CGGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAG                                                                                              <  SRR4302542.4719440/94‑1 (MQ=60)

CGGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAA                                                                                              >  SRR4302542.883003/1‑94 (MQ=60)

 GGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGC                                                                                             <  SRR4302542.6975483/94‑1 (MQ=60)

  GATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCG                                                                                            <  SRR4302542.7260820/94‑1 (MQ=60)

  GATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCG                                                                                            >  SRR4302542.59000/1‑94 (MQ=60)

   ATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGG                                                                                           <  SRR4302542.4380499/94‑1 (MQ=60)

   ATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGG                                                                                           <  SRR4302542.4720361/94‑1 (MQ=60)

    TCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGT                                                                                          >  SRR4302542.4732353/1‑94 (MQ=60)

     CGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTC                                                                                         >  SRR4302542.336828/1‑94 (MQ=60)

     CGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTC                                                                                         >  SRR4302542.579011/1‑94 (MQ=60)

      GCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCA                                                                                        >  SRR4302542.1768517/1‑94 (MQ=60)

      GCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCA                                                                                        <  SRR4302542.2342171/94‑1 (MQ=60)

      GCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCA                                                                                        <  SRR4302542.618270/94‑1 (MQ=60)

      GCTATATCGAGCAAGGCAATGTCTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCA                                                                                        <  SRR4302542.4671593/94‑1 (MQ=60)

       CTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAG                                                                                       >  SRR4302542.3691435/1‑94 (MQ=60)

        TATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGT                                                                                      >  SRR4302542.4573133/1‑94 (MQ=60)

        TATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGT                                                                                      >  SRR4302542.1234234/1‑94 (MQ=60)

        TATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGT                                                                                      <  SRR4302542.3325143/94‑1 (MQ=60)

        TATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGT                                                                                      >  SRR4302542.6743521/1‑94 (MQ=60)

         ATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTT                                                                                     >  SRR4302542.6983795/1‑94 (MQ=60)

          TATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTG                                                                                    >  SRR4302542.3934530/1‑94 (MQ=60)

          TATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTG                                                                                    <  SRR4302542.3548836/94‑1 (MQ=60)

           ATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGA                                                                                   >  SRR4302542.9188/1‑94 (MQ=60)

           ATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGA                                                                                   >  SRR4302542.4753817/1‑94 (MQ=60)

           ATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGA                                                                                   >  SRR4302542.831515/1‑94 (MQ=60)

             CGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATT                                                                                 <  SRR4302542.6161743/94‑1 (MQ=60)

              GAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTG                                                                                <  SRR4302542.308429/94‑1 (MQ=60)

               AGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGA                                                                               <  SRR4302542.6281023/94‑1 (MQ=60)

                 CAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAACCCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAAC                                                                             <  SRR4302542.2448801/94‑1 (MQ=60)

                 CAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAAC                                                                             <  SRR4302542.239015/94‑1 (MQ=60)

                 CAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAAC                                                                             >  SRR4302542.3350154/1‑94 (MQ=60)

                 CAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAAC                                                                             >  SRR4302542.6804287/1‑94 (MQ=60)

                 CAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAAC                                                                             >  SRR4302542.2224815/1‑94 (MQ=60)

                  AAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTGAGTTGATTGAACC                                                                            <  SRR4302542.3876996/94‑1 (MQ=60)

                  AAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACC                                                                            >  SRR4302542.1167013/1‑94 (MQ=60)

                  AAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACC                                                                            >  SRR4302542.57460/1‑94 (MQ=60)

                  AAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACC                                                                            >  SRR4302542.3628917/1‑94 (MQ=60)

                  AAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACC                                                                            <  SRR4302542.87362/94‑1 (MQ=60)

                   AGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCT                                                                           >  SRR4302542.4282503/1‑94 (MQ=60)

                    GGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTC                                                                          >  SRR4302542.2960591/1‑94 (MQ=60)

                     GCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCG                                                                         >  SRR4302542.1468615/1‑94 (MQ=60)

                      CAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGG                                                                        <  SRR4302542.1252916/94‑1 (MQ=60)

                      CAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGG                                                                        >  SRR4302542.4932410/1‑94 (MQ=60)

                      CAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGG                                                                        <  SRR4302542.5280774/94‑1 (MQ=60)

                       AATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGG                                                                       <  SRR4302542.4315900/94‑1 (MQ=60)

                       AATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGG                                                                       <  SRR4302542.3955521/94‑1 (MQ=60)

                       AATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGG                                                                       <  SRR4302542.3917359/94‑1 (MQ=60)

                       AATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGG                                                                       <  SRR4302542.399411/94‑1 (MQ=60)

                        ATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGA                                                                      >  SRR4302542.4659357/1‑94 (MQ=60)

                        ATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGA                                                                      >  SRR4302542.2298654/1‑94 (MQ=60)

                         TGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAA                                                                     >  SRR4302542.7020230/1‑94 (MQ=60)

                         TGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAA                                                                     >  SRR4302542.5751214/1‑94 (MQ=60)

                          GTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAG                                                                    >  SRR4302542.3843233/1‑94 (MQ=60)

                            GTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCC                                                                  >  SRR4302542.3127208/1‑94 (MQ=60)

                             TTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCG                                                                 <  SRR4302542.2306060/94‑1 (MQ=60)

                              TCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGA                                                                >  SRR4302542.3324461/1‑94 (MQ=60)

                               CCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAA                                                               >  SRR4302542.5099025/1‑94 (MQ=60)

                                CTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAG                                                              >  SRR4302542.5007654/1‑94 (MQ=60)

                                CTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAG                                                              >  SRR4302542.4618737/1‑94 (MQ=60)

                                CTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAG                                                              <  SRR4302542.830935/94‑1 (MQ=60)

                                 TTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGA                                                             >  SRR4302542.3709998/1‑94 (MQ=60)

                                  TAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGAT                                                            <  SRR4302542.7027941/94‑1 (MQ=60)

                                  TAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGAT                                                            >  SRR4302542.6109758/1‑94 (MQ=60)

                                  TAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGAT                                                            >  SRR4302542.1784704/1‑94 (MQ=60)

                                  TAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGAT                                                            <  SRR4302542.2051794/94‑1 (MQ=60)

                                   AATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATT                                                           >  SRR4302542.4854761/1‑94 (MQ=60)

                                    ATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTT                                                          <  SRR4302542.6130156/94‑1 (MQ=60)

                                    ATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTT                                                          <  SRR4302542.2303530/94‑1 (MQ=60)

                                    ATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTT                                                          >  SRR4302542.2996543/1‑94 (MQ=60)

                                     TGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTG                                                         <  SRR4302542.2309882/94‑1 (MQ=60)

                                      GGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGG                                                        <  SRR4302542.1541857/94‑1 (MQ=60)

                                      GGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGG                                                        <  SRR4302542.1375125/94‑1 (MQ=60)

                                      GGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGG                                                        <  SRR4302542.4688420/94‑1 (MQ=60)

                                       GCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGT                                                       >  SRR4302542.6118811/1‑94 (MQ=60)

                                        CAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTA                                                      >  SRR4302542.7446076/1‑94 (MQ=60)

                                        CAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTA                                                      >  SRR4302542.7291194/1‑94 (MQ=60)

                                         AAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTAC                                                     <  SRR4302542.3729189/94‑1 (MQ=60)

                                         AAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTAC                                                     <  SRR4302542.2279462/94‑1 (MQ=60)

                                         AAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTAC                                                     >  SRR4302542.6334447/1‑94 (MQ=60)

                                             GAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTGT                                                 <  SRR4302542.4914887/94‑1 (MQ=60)

                                             GAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTAT                                                 >  SRR4302542.1725684/1‑94 (MQ=60)

                                              AGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATC                                                >  SRR4302542.1739446/1‑94 (MQ=60)

                                              AGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATC                                                <  SRR4302542.2078503/94‑1 (MQ=60)

                                              AGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATC                                                >  SRR4302542.883896/1‑94 (MQ=60)

                                               GCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCG                                               <  SRR4302542.4482839/94‑1 (MQ=60)

                                                 CACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCC                                             >  SRR4302542.1079891/1‑94 (MQ=60)

                                                  ACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCC                                            >  SRR4302542.4885152/1‑94 (MQ=60)

                                                   CCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCT                                           >  SRR4302542.5288563/1‑94 (MQ=60)

                                                    CATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTG                                          >  SRR4302542.1595107/1‑94 (MQ=60)

                                                    CATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTG                                          >  SRR4302542.4872547/1‑94 (MQ=60)

                                                    CATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTG                                          >  SRR4302542.6939550/1‑94 (MQ=60)

                                                      TTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAA                                        <  SRR4302542.436576/94‑1 (MQ=60)

                                                       TGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAAC                                       >  SRR4302542.757335/1‑94 (MQ=60)

                                                          CGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAG                                    <  SRR4302542.4042648/94‑1 (MQ=60)

                                                          CGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAG                                    <  SRR4302542.3551502/94‑1 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   >  SRR4302542.3036712/1‑94 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   <  SRR4302542.6739690/94‑1 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   <  SRR4302542.567315/94‑1 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   >  SRR4302542.5348950/1‑94 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   >  SRR4302542.2834287/1‑94 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   >  SRR4302542.826568/1‑94 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   <  SRR4302542.4343631/94‑1 (MQ=60)

                                                           GATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGC                                   >  SRR4302542.5882081/1‑94 (MQ=60)

                                                            ATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCG‑CCTGAACAAGCCC                                 <  SRR4302542.2123603/94‑1 (MQ=60)

                                                            ATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCC                                  >  SRR4302542.2374986/1‑94 (MQ=60)

                                                              CAGGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCG                                <  SRR4302542.839631/94‑1 (MQ=60)

                                                                GGTGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTT                              <  SRR4302542.401178/94‑1 (MQ=60)

                                                                  TGAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGG                            <  SRR4302542.2974994/94‑1 (MQ=60)

                                                                  TGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGG                            >  SRR4302542.2884421/1‑94 (MQ=60)

                                                                   GAAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGT                           <  SRR4302542.5704228/94‑1 (MQ=60)

                                                                    AAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTA                          >  SRR4302542.6132817/1‑94 (MQ=60)

                                                                    AAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTA                          >  SRR4302542.6814250/1‑94 (MQ=60)

                                                                    AAACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTA                          >  SRR4302542.5232585/1‑94 (MQ=60)

                                                                     ACCCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTAATTATCGCCCTGAACAAGCCCGTTGGTAT                         <  SRR4302542.5716938/94‑1 (MQ=60)

                                                                     AACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTAT                         >  SRR4302542.1627583/1‑94 (MQ=60)

                                                                     AACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTAT                         <  SRR4302542.2968399/94‑1 (MQ=60)

                                                                     AACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTAT                         >  SRR4302542.6460367/1‑94 (MQ=60)

                                                                     AACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTAT                         <  SRR4302542.2746074/94‑1 (MQ=60)

                                                                      ACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATT                        >  SRR4302542.324410/1‑94 (MQ=60)

                                                                      ACCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATT                        <  SRR4302542.6410883/94‑1 (MQ=60)

                                                                       CCCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTG                       >  SRR4302542.5375574/1‑94 (MQ=60)

                                                                        CCGGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGT                      <  SRR4302542.4380893/94‑1 (MQ=60)

                                                                          GGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAA                    <  SRR4302542.6062479/94‑1 (MQ=60)

                                                                          GGCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAA                    <  SRR4302542.7249238/94‑1 (MQ=60)

                                                                           GCGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAG                   <  SRR4302542.7138597/94‑1 (MQ=60)

                                                                            CGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGC                  >  SRR4302542.5868027/1‑94 (MQ=60)

                                                                            CGACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGC                  >  SRR4302542.141672/1‑94 (MQ=60)

                                                                            CGACGTTGTAAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGC                  <  SRR4302542.6081776/94‑1 (MQ=60)

                                                                             GACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCA                 >  SRR4302542.3319106/1‑94 (MQ=60)

                                                                             GACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCA                 >  SRR4302542.3730847/1‑94 (MQ=60)

                                                                              ACGTTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCAC                >  SRR4302542.5311496/1‑94 (MQ=60)

                                                                                 TTGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGAACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCAC             >  SRR4302542.3000098/1‑94 (MQ=60)

                                                                                  TGTGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACC            >  SRR4302542.4838366/1‑94 (MQ=60)

                                                                                    TGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGA          >  SRR4302542.4182302/1‑94 (MQ=60)

                                                                                    TGAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGA          <  SRR4302542.2974830/94‑1 (MQ=60)

                                                                                     GAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAA         <  SRR4302542.5981262/94‑1 (MQ=60)

                                                                                     GAAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAA         <  SRR4302542.4678862/94‑1 (MQ=60)

                                                                                      AAAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAG        <  SRR4302542.5229082/94‑1 (MQ=60)

                                                                                      AAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAG        <  SRR4302542.5849754/94‑1 (MQ=60)

                                                                                       AAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGA       >  SRR4302542.3415418/1‑94 (MQ=60)

                                                                                       AAGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGA       >  SRR4302542.4146041/1‑94 (MQ=60)

                                                                                        AGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGAT      >  SRR4302542.6190020/1‑94 (MQ=60)

                                                                                        AGTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGAT      >  SRR4302542.7602494/1‑94 (MQ=60)

                                                                                         GTAAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATG     <  SRR4302542.2690909/94‑1 (MQ=60)

                                                                                           AAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGC   >  SRR4302542.7499383/1‑94 (MQ=60)

                                                                                           AAGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGC   >  SRR4302542.1612980/1‑94 (MQ=60)

                                                                                            AGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCG  <  SRR4302542.6106694/94‑1 (MQ=60)

                                                                                            AGCGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCG  <  SRR4302542.4485330/94‑1 (MQ=60)

CGGATCGCTATATCGAGCAAGGCAATGTGTTCCTTAATGGCAAGCGAGCCACCATTGGCGATCAGGTGAAACCCGGCGACGTTGTGAAAGTAAACGGTCAGTTGATTGAACCTCGGGAAGCCGAAGATTTGGTACTTATCGCCCTGAACAAGCCCGTTGGTATTGTAAGCACCACCGAAGATGGCG  >  NC_000913/4228003‑4228188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`