Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,247,622 A→G T164A (ACG→GCG)  malM → MalM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,247,6220AG87.9% 656.7 / 72.5 264T164A (ACG→GCG) malMMalM
Reads supporting (aligned to +/- strand):  ref base A (8/24);  new base G (123/109);  total (131/133)
Fisher's exact test for biased strand distribution p-value = 4.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 266 total aligned reads displayed.

CCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCC  >  NC_000913/4247532‑4247711
                                                                                          |                                                                                         
ccAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACcgcg                                                                                         >  2:5036923/1‑93 (MQ=255)
 cAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCgg                                                                                        >  1:7345691/1‑93 (MQ=255)
 cAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCgg                                                                                        >  2:225615/1‑93 (MQ=255)
 cAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACgg                                                                                        >  1:6334448/1‑93 (MQ=255)
    gCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGaaa                                                                                     >  2:6529307/1‑93 (MQ=255)
      gTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGaaaaa                                                                                   >  1:3269120/1‑93 (MQ=255)
      gTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACAGCGGaaaaa                                                                                   >  1:3836642/1‑93 (MQ=255)
       tgatgaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAg                                                                                  >  1:1089784/1‑93 (MQ=255)
       tgatgaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAg                                                                                  <  2:1921966/93‑1 (MQ=255)
        gatgaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGa                                                                                 >  1:1350975/1‑93 (MQ=255)
        gatgaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGa                                                                                 >  1:5857758/1‑93 (MQ=255)
          tgaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGAtc                                                                               <  1:6874565/93‑1 (MQ=255)
          tgaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGAtc                                                                               <  1:4546952/93‑1 (MQ=255)
          tgaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGAtc                                                                               >  1:3562582/1‑93 (MQ=255)
           gaGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGAtct                                                                              <  1:4059560/93‑1 (MQ=255)
            aGTGCAGATCGGCTGGAAGGCGTTATGGGCCTGACACCGGGGTTGGGGCAGCAAAAACTTTTTTTTCTGG‑CTTTACCGCGGAAAAAGATCTcc                                                                            <  1:2395347/93‑1 (MQ=255)
             gTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTcc                                                                            <  1:3408602/93‑1 (MQ=255)
             gTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTcc                                                                            >  1:3641937/1‑93 (MQ=255)
             gTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTcc                                                                            <  2:7050223/93‑1 (MQ=255)
              tGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCca                                                                           <  1:4019948/93‑1 (MQ=255)
               gCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCcag                                                                          <  2:1105199/93‑1 (MQ=255)
                 aGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCcagca                                                                        >  2:1684838/1‑93 (MQ=255)
                 aGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCcagca                                                                        <  2:3725321/93‑1 (MQ=255)
                  gATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCcagcag                                                                       >  1:6055170/1‑93 (MQ=255)
                  gATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCcagcag                                                                       >  1:4906287/1‑93 (MQ=255)
                    tCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgac                                                                     <  1:3122884/93‑1 (MQ=255)
                    tCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgac                                                                     <  1:7406704/93‑1 (MQ=255)
                     cGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgacg                                                                    <  1:6456058/93‑1 (MQ=255)
                      ggCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgacga                                                                   >  2:1331912/1‑93 (MQ=255)
                      ggCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgacga                                                                   >  1:5783406/1‑93 (MQ=255)
                       gCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgacgac                                                                  <  2:3628368/93‑1 (MQ=255)
                       gCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgacgac                                                                  >  2:2519403/1‑93 (MQ=255)
                       gCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAgacgac                                                                  >  2:4018105/1‑93 (MQ=255)
                        cTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGAcc                                                                 <  1:5499433/93‑1 (MQ=255)
                         tGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGAccc                                                                <  1:5374750/93‑1 (MQ=255)
                          ggAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCa                                                               >  2:207112/1‑93 (MQ=255)
                           gAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCaa                                                              >  1:3946441/1‑93 (MQ=255)
                           gAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCaa                                                              <  1:1023871/93‑1 (MQ=255)
                            aaGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAAc                                                             <  2:6307805/93‑1 (MQ=255)
                            aaGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAAc                                                             <  1:1613636/93‑1 (MQ=255)
                             aGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACt                                                            >  1:5653387/1‑93 (MQ=255)
                             aGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACt                                                            <  2:6271145/93‑1 (MQ=255)
                             aGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACt                                                            <  1:3253413/93‑1 (MQ=255)
                              ggCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTg                                                           >  1:6720533/1‑93 (MQ=255)
                                cGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCt                                                         <  2:7506021/93‑1 (MQ=255)
                                  ttATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCg                                                       >  2:1850010/1‑93 (MQ=255)
                                   tATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGa                                                      >  2:4352785/1‑93 (MQ=255)
                                     tGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGAcc                                                    >  2:5348440/1‑93 (MQ=255)
                                         ccTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGc                                                >  1:3058711/1‑93 (MQ=255)
                                          cTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCt                                               >  2:1043363/1‑93 (MQ=255)
                                             acacCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTaaa                                            <  2:5799766/93‑1 (MQ=255)
                                             acacCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTaaa                                            <  1:5901215/93‑1 (MQ=255)
                                              cacCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAg                                           <  1:2030233/93‑1 (MQ=255)
                                                 cGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCt                                        <  2:3453077/93‑1 (MQ=255)
                                                  ggCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTa                                       <  1:629813/93‑1 (MQ=255)
                                                  ggCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTa                                       <  1:615591/93‑1 (MQ=255)
                                                  ggCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTa                                       <  2:3960500/93‑1 (MQ=255)
                                                   gCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTAt                                      >  2:5789164/1‑93 (MQ=255)
                                                   gCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTAt                                      <  1:3712221/93‑1 (MQ=255)
                                                   gCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTAt                                      <  1:4609156/93‑1 (MQ=255)
                                                     gTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGc                                    >  2:4878922/1‑93 (MQ=255)
                                                     gTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGc                                    <  1:6191957/93‑1 (MQ=255)
                                                     gTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGc                                    >  2:38823/1‑93 (MQ=255)
                                                       tGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCa                                  <  2:4776707/93‑1 (MQ=255)
                                                          ggCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAgg                               <  2:6991198/93‑1 (MQ=255)
                                                          ggCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAgg                               <  1:3424171/93‑1 (MQ=255)
                                                           gcagcaAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAggg                              >  2:1419109/1‑93 (MQ=255)
                                                           gcagcaAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAggg                              >  1:2791256/1‑93 (MQ=255)
                                                           gcagcaAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAggg                              >  1:7124187/1‑93 (MQ=255)
                                                              gcaAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGt                           <  2:3205778/93‑1 (MQ=255)
                                                              gcaAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGt                           >  1:1541609/1‑93 (MQ=255)
                                                              gcaAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGt                           >  2:1222664/1‑93 (MQ=255)
                                                              gcaAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGt                           >  2:5164265/1‑93 (MQ=255)
                                                                aaaaaCTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCg                         >  2:2595865/1‑93 (MQ=255)
                                                                    aCTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTaa                     >  2:2965705/1‑93 (MQ=255)
                                                                    aCTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTaa                     <  2:5519982/93‑1 (MQ=255)
                                                                     cTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAAc                    <  1:1245294/93‑1 (MQ=255)
                                                                      tttATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACt                   >  2:618847/1‑93 (MQ=255)
                                                                      tttATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACt                   <  2:6861807/93‑1 (MQ=255)
                                                                       ttATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTc                  <  2:3032525/93‑1 (MQ=255)
                                                                       ttATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTc                  <  2:6759441/93‑1 (MQ=255)
                                                                       ttATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTc                  <  1:1914881/93‑1 (MQ=255)
                                                                        tATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCg                 >  1:803716/1‑93 (MQ=255)
                                                                          tGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGAt               >  2:5455049/1‑93 (MQ=255)
                                                                          tGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGAt               >  1:4721742/1‑93 (MQ=255)
                                                                          tGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGAt               <  2:4687359/93‑1 (MQ=255)
                                                                           gTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATc              >  1:2168353/1‑93 (MQ=255)
                                                                            ttCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATcc             <  2:5712686/93‑1 (MQ=255)
                                                                            ttCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATcc             <  1:6904849/93‑1 (MQ=255)
                                                                             tCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATccc            <  1:4807684/93‑1 (MQ=255)
                                                                             tCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATccc            <  2:240689/93‑1 (MQ=255)
                                                                                ggTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGa         <  2:109003/93‑1 (MQ=255)
                                                                                 gTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGat        >  2:2290/1‑93 (MQ=255)
                                                                                    tttACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCCAGGGCGTCGGTAACTCGATCCCGGATATc     >  2:7729/1‑93 (MQ=255)
                                                                                    tttACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATc     >  2:1078760/1‑93 (MQ=255)
                                                                                    tttACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATc     >  2:6828687/1‑93 (MQ=255)
                                                                                    tttACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATc     >  1:2620139/1‑93 (MQ=255)
                                                                                      tACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATccc   <  2:7282262/93‑1 (MQ=255)
                                                                                      tACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATccc   >  2:926440/1‑93 (MQ=255)
                                                                                      tACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATccc   <  1:1688723/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCC  >  NC_000913/4247532‑4247711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAACCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCCGATC  >  NC_000913/4247529‑4247715
                                                                                             |                                                                                             
GAACCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCG                                                                                               <  SRR4302542.6455294/94‑1 (MQ=60)
GAACCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCA                                                                                               <  SRR4302542.3115165/94‑1 (MQ=60)
 AACCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGC                                                                                              >  SRR4302542.2833577/1‑94 (MQ=60)
 AACCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGC                                                                                              <  SRR4302542.2120004/94‑1 (MQ=60)
   CCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGG                                                                                            >  SRR4302542.3338950/1‑94 (MQ=60)
   CCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGG                                                                                            <  SRR4302542.2036892/94‑1 (MQ=60)
   CCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGG                                                                                            >  SRR4302542.7417636/1‑94 (MQ=60)
   CCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGG                                                                                            >  SRR4302542.6396860/1‑94 (MQ=60)
     AGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAA                                                                                          <  SRR4302542.7141330/94‑1 (MQ=60)
        CGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAA                                                                                       >  SRR4302542.3299979/1‑94 (MQ=60)
        CGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACAGCGGAAAAA                                                                                       >  SRR4302542.3874583/1‑94 (MQ=60)
         GTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAG                                                                                      >  SRR4302542.1099123/1‑94 (MQ=60)
          TGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGA                                                                                     >  SRR4302542.5914560/1‑94 (MQ=60)
          TGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGA                                                                                     >  SRR4302542.1362644/1‑94 (MQ=60)
          TGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGA                                                                                     >  SRR4302542.1875140/1‑94 (MQ=60)
           GATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGAT                                                                                    <  SRR4302542.380860/94‑1 (MQ=60)
            ATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATC                                                                                   <  SRR4302542.3123953/94‑1 (MQ=60)
            ATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATC                                                                                   >  SRR4302542.3595842/1‑94 (MQ=60)
             TGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCT                                                                                  <  SRR4302542.4591461/94‑1 (MQ=60)
             TGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCT                                                                                  <  SRR4302542.6942095/94‑1 (MQ=60)
             TGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCA                                                                                  <  SRR4302542.1734852/94‑1 (MQ=60)
              GAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTC                                                                                 <  SRR4302542.4099593/94‑1 (MQ=60)
               AGTGCAGATCGGCTGGAAGGCGTTATGGGCCTGACACCGGGGTTGGGGCAGCAAAAACTTTTTTTTCTGG‑CTTTACCGCGGAAAAAGATCTCCC                                                                               <  SRR4302542.2418386/94‑1 (MQ=60)
               AGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCC                                                                                >  SRR4302542.3675912/1‑94 (MQ=60)
                GTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCA                                                                               <  SRR4302542.6256566/94‑1 (MQ=60)
                GTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCA                                                                               <  SRR4302542.3440553/94‑1 (MQ=60)
                 TGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAG                                                                              <  SRR4302542.4059618/94‑1 (MQ=60)
                    AGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAG                                                                           >  SRR4302542.6113783/1‑94 (MQ=60)
                    AGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAG                                                                           >  SRR4302542.4954011/1‑94 (MQ=60)
                    AGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAG                                                                           <  SRR4302542.4074221/94‑1 (MQ=60)
                    AGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAAATTTTTGTTCTGGTCTTTACCGCGGAAAAAAATCTCCAGCAG                                                                           >  SRR4302542.5189202/1‑94 (MQ=60)
                     GATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGA                                                                          >  SRR4302542.167502/1‑94 (MQ=60)
                      ATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGAC                                                                         <  SRR4302542.6028250/94‑1 (MQ=60)
                       TCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACG                                                                        <  SRR4302542.3152587/94‑1 (MQ=60)
                       TCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACG                                                                        <  SRR4302542.7479193/94‑1 (MQ=60)
                        CGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGA                                                                       >  SRR4302542.5839563/1‑94 (MQ=60)
                        CGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGA                                                                       <  SRR4302542.6519623/94‑1 (MQ=60)
                         GGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGAC                                                                      <  SRR4302542.3445010/94‑1 (MQ=60)
                           CTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCC                                                                    >  SRR4302542.6007392/1‑94 (MQ=60)
                           CTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCC                                                                    <  SRR4302542.5552851/94‑1 (MQ=60)
                            TGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCA                                                                   <  SRR4302542.6291666/94‑1 (MQ=60)
                            TGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCA                                                                   <  SRR4302542.5426976/94‑1 (MQ=60)
                             GGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAA                                                                  >  SRR4302542.3985410/1‑94 (MQ=60)
                             GGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAA                                                                  <  SRR4302542.5932341/94‑1 (MQ=60)
                              GAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAAC                                                                 >  SRR4302542.5767645/1‑94 (MQ=60)
                              GAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAAC                                                                 <  SRR4302542.1032695/94‑1 (MQ=60)
                              GAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAAC                                                                 <  SRR4302542.2962541/94‑1 (MQ=60)
                               AAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACT                                                                <  SRR4302542.6938370/94‑1 (MQ=60)
                               AAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACT                                                                <  SRR4302542.1627577/94‑1 (MQ=60)
                               AAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACT                                                                >  SRR4302542.3528498/1‑94 (MQ=60)
                               AAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACT                                                                >  SRR4302542.5708275/1‑94 (MQ=60)
                               AAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACT                                                                <  SRR4302542.4533947/94‑1 (MQ=60)
                                AGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTG                                                               <  SRR4302542.3284141/94‑1 (MQ=60)
                                AGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTG                                                               >  SRR4302542.6786718/1‑94 (MQ=60)
                                  GCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCT                                                             <  SRR4302542.4027017/94‑1 (MQ=60)
                                      NNTGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGAC                                                         <  SRR4302542.1593194/94‑1 (MQ=60)
                                       ATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACC                                                        >  SRR4302542.3405858/1‑94 (MQ=60)
                                        TGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCC                                                       <  SRR4302542.3114000/94‑1 (MQ=60)
                                          CGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGG                                                     <  SRR4302542.6164389/94‑1 (MQ=60)
                                           GCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGC                                                    >  SRR4302542.3087882/1‑94 (MQ=60)
                                            CCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCT                                                   <  SRR4302542.6958333/94‑1 (MQ=60)
                                             CTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCACCTGCTCGACTCGGCTA                                                  >  SRR4302542.3295568/1‑94 (MQ=60)
                                             CTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTA                                                  >  SRR4302542.3295569/1‑94 (MQ=60)
                                               GACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAA                                                >  SRR4302542.6455305/1‑94 (MQ=60)
                                                ACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAG                                               <  SRR4302542.7588125/94‑1 (MQ=60)
                                                ACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAG                                               <  SRR4302542.729509/94‑1 (MQ=60)
                                                ACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAG                                               <  SRR4302542.5958424/94‑1 (MQ=60)
                                                ACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAG                                               <  SRR4302542.2840543/94‑1 (MQ=60)
                                                ACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAG                                               <  SRR4302542.5508653/94‑1 (MQ=60)
                                                 CACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGC                                              >  SRR4302542.7511488/1‑94 (MQ=60)
                                                 CACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGC                                              <  SRR4302542.2047801/94‑1 (MQ=60)
                                                  ACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCC                                             >  SRR4302542.7265662/1‑94 (MQ=60)
                                                  ACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCC                                             <  SRR4302542.3939455/94‑1 (MQ=60)
                                                   CCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCT                                            <  SRR4302542.3658645/94‑1 (MQ=60)
                                                    CGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTA                                           >  SRR4302542.6373408/1‑94 (MQ=60)
                                                     GGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTAT                                          <  SRR4302542.620881/94‑1 (MQ=60)
                                                     GGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTAT                                          <  SRR4302542.635238/94‑1 (MQ=60)
                                                     GGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTAT                                          <  SRR4302542.4605144/94‑1 (MQ=60)
                                                     GGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTAT                                          >  SRR4302542.6697476/1‑94 (MQ=60)
                                                      GCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATG                                         <  SRR4302542.4654198/94‑1 (MQ=60)
                                                      GCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATG                                         <  SRR4302542.3633623/94‑1 (MQ=60)
                                                      GCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATG                                         <  SRR4302542.3748943/94‑1 (MQ=60)
                                                       CGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGC                                        >  SRR4302542.7434494/1‑94 (MQ=60)
                                                        GTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCC                                       >  SRR4302542.3229122/1‑94 (MQ=60)
                                                        GTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCC                                       <  SRR4302542.6252204/94‑1 (MQ=60)
                                                        GTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCC                                       >  SRR4302542.4713991/1‑94 (MQ=60)
                                                         TTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCA                                      <  SRR4302542.3692876/94‑1 (MQ=60)
                                                          TGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAA                                     <  SRR4302542.2433446/94‑1 (MQ=60)
                                                           GGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAG                                    <  SRR4302542.549616/94‑1 (MQ=60)
                                                           GGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAG                                    <  SRR4302542.1609496/94‑1 (MQ=60)
                                                           GGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAG                                    >  SRR4302542.2369297/1‑94 (MQ=60)
                                                           GGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAG                                    <  SRR4302542.5812567/94‑1 (MQ=60)
                                                             GGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGG                                  >  SRR4302542.7194110/1‑94 (MQ=60)
                                                             GGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGG                                  <  SRR4302542.3456244/94‑1 (MQ=60)
                                                             GGCAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGG                                  >  SRR4302542.2818122/1‑94 (MQ=60)
                                                               CAGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCG                                <  SRR4302542.1106619/94‑1 (MQ=60)
                                                                AGCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGT                               >  SRR4302542.1554923/1‑94 (MQ=60)
                                                                 GCAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTC                              <  SRR4302542.275348/94‑1 (MQ=60)
                                                                  CAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCG                             >  SRR4302542.4517909/1‑94 (MQ=60)
                                                                  CAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCG                             >  SRR4302542.2235396/1‑94 (MQ=60)
                                                                  CAAAAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGACTAAAGCCTATGCCAAGGGCGTCG                             >  SRR4302542.891407/1‑94 (MQ=60)
                                                                    AAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGT                           <  SRR4302542.862380/94‑1 (MQ=60)
                                                                     AAACTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTA                          <  SRR4302542.1214985/94‑1 (MQ=60)
                                                                        CTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACT                       <  SRR4302542.1256001/94‑1 (MQ=60)
                                                                        CTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACT                       >  SRR4302542.2004818/1‑94 (MQ=60)
                                                                        CTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACT                       <  SRR4302542.5907510/94‑1 (MQ=60)
                                                                        CTTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACT                       >  SRR4302542.5134810/1‑94 (MQ=60)
                                                                        CTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACT                       <  SRR4302542.1483265/94‑1 (MQ=60)
                                                                         TTTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTC                      >  SRR4302542.5646202/1‑94 (MQ=60)
                                                                          TTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCG                     <  SRR4302542.6375773/94‑1 (MQ=60)
                                                                          TTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCG                     <  SRR4302542.6819426/94‑1 (MQ=60)
                                                                          TTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCG                     <  SRR4302542.1931430/94‑1 (MQ=60)
                                                                          TTATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCG                     >  SRR4302542.810628/1‑94 (MQ=60)
                                                                           TATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGA                    <  SRR4302542.4876628/94‑1 (MQ=60)
                                                                            ATGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGAT                   >  SRR4302542.4767765/1‑94 (MQ=60)
                                                                             TGTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATC                  <  SRR4302542.1501602/94‑1 (MQ=60)
                                                                             TGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATC                  >  SRR4302542.2187026/1‑94 (MQ=60)
                                                                              GTTCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCC                 >  SRR4302542.4780514/1‑94 (MQ=60)
                                                                               TTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCC                <  SRR4302542.6972637/94‑1 (MQ=60)
                                                                                TCTGGTCTTTCCCGCGGAAAAAGACCTCCGGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCG               <  SRR4302542.6239064/94‑1 (MQ=60)
                                                                                TCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCG               >  SRR4302542.1357044/1‑94 (MQ=60)
                                                                                TCTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCG               <  SRR4302542.4854517/94‑1 (MQ=60)
                                                                                TCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCG               <  SRR4302542.3974894/94‑1 (MQ=60)
                                                                                 CTGGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGG              <  SRR4302542.7023794/94‑1 (MQ=60)
                                                                                   GGTCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGAT            <  SRR4302542.4090078/94‑1 (MQ=60)
                                                                                   GGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGAT            >  SRR4302542.6192350/1‑94 (MQ=60)
                                                                                     TCTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATAT          <  SRR4302542.6630426/94‑1 (MQ=60)
                                                                                      CTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATC         >  SRR4302542.3534166/1‑94 (MQ=60)
                                                                                      CTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATC         >  SRR4302542.1036873/1‑94 (MQ=60)
                                                                                      CTTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATC         >  SRR4302542.2645460/1‑94 (MQ=60)
                                                                                       TTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCC        >  SRR4302542.2096218/1‑94 (MQ=60)
                                                                                       TTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCC        >  SRR4302542.3791656/1‑94 (MQ=60)
                                                                                       TTTACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCC        <  SRR4302542.4812041/94‑1 (MQ=60)
                                                                                       TTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCC        >  SRR4302542.584062/1‑94 (MQ=60)
                                                                                         TACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCC      >  SRR4302542.4655160/1‑94 (MQ=60)
                                                                                         TACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCC      <  SRR4302542.1703344/94‑1 (MQ=60)
                                                                                          ACCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCCG     <  SRR4302542.2511620/94‑1 (MQ=60)
                                                                                           CCGCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCCGA    >  SRR4302542.3172589/1‑94 (MQ=60)
                                                                                             GCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCCGATC  <  SRR4302542.63347/94‑1 (MQ=60)
                                                                                             GCGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCCGATC  <  SRR4302542.4934312/94‑1 (MQ=60)
                                                                                             |                                                                                             
GAACCAGGCGTGATGAGTGCAGATCGGCTGGAAGGCGTTATGCGCCTGACACCGGCGTTGGGGCAGCAAAAACTTTATGTTCTGGTCTTTACCACGGAAAAAGATCTCCAGCAGACGACCCAACTGCTCGACCCGGCTAAAGCCTATGCCAAGGGCGTCGGTAACTCGATCCCGGATATCCCCGATC  >  NC_000913/4247529‑4247715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑