Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,316,203 T→G Q47P (CAG→CCG)  phnK ← ATP‑binding component of phosphonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,316,2030TG86.0% 672.4 / 97.9 286Q47P (CAG→CCG) phnKATP‑binding component of phosphonate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (121/125);  minor base A (21/19);  total (142/144)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 288 total aligned reads displayed.

CGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316113‑4316292
                                                                                          |                                                                                         
cggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGc                                                                                         >  2:2795254/1‑93 (MQ=255)
  gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGt                                                                                       >  1:394558/1‑93 (MQ=255)
   tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTg                                                                                      >  2:5737307/1‑93 (MQ=255)
    cGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTgg                                                                                     >  2:2022394/1‑93 (MQ=255)
      gCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTc                                                                                   <  1:5523360/93‑1 (MQ=255)
       ccTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCt                                                                                  <  2:3808448/93‑1 (MQ=255)
        cTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCtt                                                                                 >  2:3116800/1‑93 (MQ=255)
         tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTc                                                                                >  1:725700/1‑93 (MQ=255)
         tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTc                                                                                <  2:407292/93‑1 (MQ=255)
         tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTc                                                                                <  2:4911279/93‑1 (MQ=255)
         tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                <  2:5453797/93‑1 (MQ=255)
          cGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                               >  2:519573/1‑93 (MQ=255)
           gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTccc                                                                              <  2:6231210/93‑1 (MQ=255)
            cTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCg                                                                             >  1:4858846/1‑93 (MQ=255)
            cTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCg                                                                             <  1:875886/93‑1 (MQ=255)
             tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCgg                                                                            <  2:1102453/93‑1 (MQ=255)
             tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCgg                                                                            >  2:4408570/1‑93 (MQ=255)
             tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCgg                                                                            >  1:2211301/1‑93 (MQ=255)
             tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCgg                                                                            <  2:7376650/93‑1 (MQ=255)
              cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGa                                                                           >  2:2369723/1‑93 (MQ=255)
              cATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGa                                                                           <  1:5181234/93‑1 (MQ=255)
                ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGc                                                                         >  1:6948126/1‑93 (MQ=255)
                ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGc                                                                         <  1:4974347/93‑1 (MQ=255)
                ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGc                                                                         <  1:5557009/93‑1 (MQ=255)
                 tGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGcc                                                                        >  1:252446/1‑93 (MQ=255)
                 tGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCCCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGcc                                                                        <  1:7507437/93‑1 (MQ=255)
                   cATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCgg                                                                      >  1:5027454/1‑93 (MQ=255)
                    aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGa                                                                     >  1:93888/1‑93 (MQ=255)
                    aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGa                                                                     >  2:3504798/1‑93 (MQ=255)
                     tACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGAt                                                                    >  2:2612275/1‑93 (MQ=255)
                      aCAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGAtt                                                                   >  1:1201067/1‑93 (MQ=255)
                       cAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTc                                                                  >  1:4083/1‑93 (MQ=255)
                        aGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTcc                                                                 >  2:3867691/1‑93 (MQ=255)
                           gAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCg                                                              >  1:3830535/1‑93 (MQ=255)
                            aaCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGa                                                             >  1:4882048/1‑93 (MQ=255)
                            aaCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGa                                                             >  2:251467/1‑93 (MQ=255)
                            aaCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGa                                                             >  2:7249144/1‑93 (MQ=255)
                            aaCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGa                                                             >  1:1035496/1‑93 (MQ=255)
                             aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc                                                            <  2:6807030/93‑1 (MQ=255)
                                 ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                        >  2:2228520/1‑93 (MQ=255)
                                  tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGc                                                       >  2:2732784/1‑93 (MQ=255)
                                   ctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc                                                      <  2:5429747/93‑1 (MQ=255)
                                    tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     <  2:7100783/93‑1 (MQ=255)
                                    tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     <  1:1088135/93‑1 (MQ=255)
                                     cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa                                                    <  1:1841318/93‑1 (MQ=255)
                                      gTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCGGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   <  1:5092855/93‑1 (MQ=255)
                                      gTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   >  1:5613007/1‑93 (MQ=255)
                                        aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                 <  2:703473/93‑1 (MQ=255)
                                        aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                 <  1:5323642/93‑1 (MQ=255)
                                        aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                 <  2:4141982/93‑1 (MQ=255)
                                         gTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc                                                <  2:6689725/93‑1 (MQ=255)
                                            aaTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGCCTTCAGCGGCGTGGTATTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             <  1:3324161/93‑1 (MQ=255)
                                             aTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcc                                            <  1:1677718/93‑1 (MQ=255)
                                              tttCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc                                           >  1:538811/1‑93 (MQ=255)
                                               ttCCCCCTGCTGCGGCGTCAGGCGCGCGGAAATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcccc                                          <  1:3186666/93‑1 (MQ=255)
                                                tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc                                         >  1:2903233/1‑93 (MQ=255)
                                                  ccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCgg                                       >  2:1199445/1‑93 (MQ=255)
                                                  ccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCgg                                       >  2:2471553/1‑93 (MQ=255)
                                                     ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  1:6285896/1‑93 (MQ=255)
                                                     ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  1:6604460/1‑93 (MQ=255)
                                                       gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  >  2:1521599/1‑93 (MQ=255)
                                                       gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  <  2:5579051/93‑1 (MQ=255)
                                                         tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaaa                                <  1:2332100/93‑1 (MQ=255)
                                                         tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaaa                                <  1:452657/93‑1 (MQ=255)
                                                         tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaaa                                >  1:7179830/1‑93 (MQ=255)
                                                         tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaaa                                >  1:1495539/1‑93 (MQ=255)
                                                               gTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa                          >  2:5238028/1‑93 (MQ=255)
                                                               gTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa                          <  1:3504798/93‑1 (MQ=255)
                                                                tCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag                         <  2:1732884/93‑1 (MQ=255)
                                                                tCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag                         >  1:4447948/1‑93 (MQ=255)
                                                                  aGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAagag                       >  1:6463109/1‑93 (MQ=255)
                                                                   ggCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  1:4682152/93‑1 (MQ=255)
                                                                   ggCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  1:2023578/93‑1 (MQ=255)
                                                                   ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  1:3094324/93‑1 (MQ=255)
                                                                     cgcgcgGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                    <  1:2401994/93‑1 (MQ=255)
                                                                     cgcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                    <  2:326150/93‑1 (MQ=255)
                                                                      gcgcgGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt                   <  1:6513211/93‑1 (MQ=255)
                                                                      gcgcgGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt                   <  2:4144161/93‑1 (MQ=255)
                                                                      gcgcgGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt                   <  2:2745800/93‑1 (MQ=255)
                                                                      gcgcgGAGATCGACTCCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGGCAATGCCCAGCGCCTCCCCCGGCCATAAATCAAAATAGACAt                   <  1:6102771/93‑1 (MQ=255)
                                                                       cgcgGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATc                  >  1:438375/1‑93 (MQ=255)
                                                                       cgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATc                  <  2:7502788/93‑1 (MQ=255)
                                                                        gcgGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCg                 >  2:4241499/1‑93 (MQ=255)
                                                                         cgGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGc                <  1:4227848/93‑1 (MQ=255)
                                                                            agaTCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaa             <  1:5975342/93‑1 (MQ=255)
                                                                            agaTCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaa             <  2:4982438/93‑1 (MQ=255)
                                                                             nntCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa            <  1:3717046/91‑1 (MQ=255)
                                                                              aTCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAg           >  2:1366414/1‑93 (MQ=255)
                                                                              aTCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAg           <  2:2944096/93‑1 (MQ=255)
                                                                                cGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGcc         >  2:5977398/1‑93 (MQ=255)
                                                                                 gACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  2:1802992/93‑1 (MQ=255)
                                                                                 gACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  2:1909551/93‑1 (MQ=255)
                                                                                 gACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  2:4830552/93‑1 (MQ=255)
                                                                                 gACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  2:6405401/93‑1 (MQ=255)
                                                                                   cTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      >  2:861732/1‑93 (MQ=255)
                                                                                   cTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCttt      <  1:6726850/93‑1 (MQ=255)
                                                                                    ttCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTg     <  2:2153300/93‑1 (MQ=255)
                                                                                    ttCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTg     >  1:986792/1‑93 (MQ=255)
                                                                                    ttCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTg     >  2:2347235/1‑93 (MQ=255)
                                                                                       aGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGccc  <  2:5796186/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316113‑4316292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTGCCCGGCG  >  NC_000913/4316110‑4316296
                                                                                             |                                                                                              
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCG                                                                                                <  SRR4302542.5801377/94‑1 (MQ=60)
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCA                                                                                                >  SRR4302542.4402900/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGT                                                                                            >  SRR4302542.6180909/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGT                                                                                            >  SRR4302542.6052262/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGT                                                                                            >  SRR4302542.397973/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGT                                                                                            >  SRR4302542.2074384/1‑94 (MQ=60)
     GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTG                                                                                           <  SRR4302542.6481088/94‑1 (MQ=60)
     GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTG                                                                                           >  SRR4302542.1565369/1‑94 (MQ=60)
     GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTG                                                                                           <  SRR4302542.5060994/94‑1 (MQ=60)
       CGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGT                                                                                         <  SRR4302542.2568651/94‑1 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTC                                                                                        <  SRR4302542.4316261/94‑1 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTC                                                                                        >  SRR4302542.4428517/1‑94 (MQ=60)
         GCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCT                                                                                       <  SRR4302542.5577042/94‑1 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTC                                                                                     >  SRR4302542.1585880/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTC                                                                                     >  SRR4302542.5285463/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTC                                                                                     >  SRR4302542.4009475/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTC                                                                                     >  SRR4302542.731954/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                     >  SRR4302542.1791517/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCG                                                                                  >  SRR4302542.3968323/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCG                                                                                  <  SRR4302542.7294706/94‑1 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCG                                                                                  >  SRR4302542.4906167/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCG                                                                                  >  SRR4302542.4770693/1‑94 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGG                                                                                 >  SRR4302542.2230333/1‑94 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGG                                                                                 <  SRR4302542.883441/94‑1 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGG                                                                                 >  SRR4302542.6995070/1‑94 (MQ=60)
                TCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGA                                                                                >  SRR4302542.957468/1‑94 (MQ=60)
                 CATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAG                                                                               <  SRR4302542.7585911/94‑1 (MQ=60)
                 CATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAG                                                                               <  SRR4302542.5231574/94‑1 (MQ=60)
                  ATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGC                                                                              >  SRR4302542.7016397/1‑94 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCC                                                                             <  SRR4302542.5022640/94‑1 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCC                                                                             >  SRR4302542.674663/1‑94 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCC                                                                             <  SRR4302542.5610999/94‑1 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCC                                                                             >  SRR4302542.254602/1‑94 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCC                                                                             <  SRR4302542.5104565/94‑1 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCC                                                                             >  SRR4302542.4054803/1‑94 (MQ=60)
                    TGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCCCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCG                                                                            <  SRR4302542.7581273/94‑1 (MQ=60)
                     GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGG                                                                           <  SRR4302542.4472596/94‑1 (MQ=60)
                     GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGG                                                                           >  SRR4302542.5076259/1‑94 (MQ=60)
                      CATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGA                                                                          >  SRR4302542.94683/1‑94 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATT                                                                        >  SRR4302542.3016730/1‑94 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATT                                                                        <  SRR4302542.1394480/94‑1 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATT                                                                        >  SRR4302542.1211408/1‑94 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                        <  SRR4302542.2041595/94‑1 (MQ=60)
                         ACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTC                                                                       >  SRR4302542.4114/1‑94 (MQ=60)
                         ACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTC                                                                       >  SRR4302542.37709/1‑94 (MQ=60)
                          CAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCC                                                                      >  SRR4302542.2025583/1‑94 (MQ=60)
                          CAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCC                                                                      >  SRR4302542.5560477/1‑94 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                   <  SRR4302542.3091839/94‑1 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                   <  SRR4302542.5055743/94‑1 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                   >  SRR4302542.3868423/1‑94 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                   >  SRR4302542.6532298/1‑94 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                  >  SRR4302542.4929553/1‑94 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                  >  SRR4302542.1044398/1‑94 (MQ=60)
                               AACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGAC                                                                 >  SRR4302542.1135098/1‑94 (MQ=60)
                                   GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATG                                                             <  SRR4302542.898651/94‑1 (MQ=60)
                                    TTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGC                                                            >  SRR4302542.978367/1‑94 (MQ=60)
                                      CTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCC                                                          >  SRR4302542.2238033/1‑94 (MQ=60)
                                      CTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCC                                                          >  SRR4302542.6957355/1‑94 (MQ=60)
                                       TCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCA                                                         <  SRR4302542.1097464/94‑1 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                        <  SRR4302542.5194593/94‑1 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                        >  SRR4302542.5667541/1‑94 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                        <  SRR4302542.6142811/94‑1 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                        <  SRR4302542.1857257/94‑1 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCGGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                       <  SRR4302542.5142348/94‑1 (MQ=60)
                                           AGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAC                                                     <  SRR4302542.5375360/94‑1 (MQ=60)
                                            GTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACT                                                    <  SRR4302542.655962/94‑1 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGCCTTCAGCGGCGTGGTATTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                                 <  SRR4302542.3355461/94‑1 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                                 >  SRR4302542.3188927/1‑94 (MQ=60)
                                                ATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCC                                                >  SRR4302542.543472/1‑94 (MQ=60)
                                                ATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCC                                                <  SRR4302542.1692221/94‑1 (MQ=60)
                                                 TTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCC                                               >  SRR4302542.5666411/1‑94 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCC                                              <  SRR4302542.6602660/94‑1 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCC                                              >  SRR4302542.3538281/1‑94 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCC                                              >  SRR4302542.2931098/1‑94 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAAATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCC                                              <  SRR4302542.3216854/94‑1 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCG                                             >  SRR4302542.3472424/1‑94 (MQ=60)
                                                    CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGG                                            >  SRR4302542.2500703/1‑94 (MQ=60)
                                                    CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGG                                            >  SRR4302542.3150711/1‑94 (MQ=60)
                                                      CCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCC                                          >  SRR4302542.423357/1‑94 (MQ=60)
                                                      CCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCC                                          >  SRR4302542.3954839/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                         >  SRR4302542.6669530/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                         >  SRR4302542.6347837/1‑94 (MQ=60)
                                                         TGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATA                                       >  SRR4302542.2249238/1‑94 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                      >  SRR4302542.5247435/1‑94 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                      >  SRR4302542.441161/1‑94 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                      >  SRR4302542.5684898/1‑94 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                     >  SRR4302542.7250251/1‑94 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                     <  SRR4302542.4693586/94‑1 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                     >  SRR4302542.1508475/1‑94 (MQ=60)
                                                            TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                    <  SRR4302542.2352080/94‑1 (MQ=60)
                                                            TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                    <  SRR4302542.456564/94‑1 (MQ=60)
                                                            TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                    <  SRR4302542.5136242/94‑1 (MQ=60)
                                                            TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                    >  SRR4302542.6584092/1‑94 (MQ=60)
                                                             GCGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATC                                   <  SRR4302542.5656172/94‑1 (MQ=60)
                                                              CGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCA                                  <  SRR4302542.4879817/94‑1 (MQ=60)
                                                              CGGCGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCA                                  <  SRR4302542.4671229/94‑1 (MQ=60)
                                                                GCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAA                                <  SRR4302542.902682/94‑1 (MQ=60)
                                                                 CGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAA                               >  SRR4302542.5749612/1‑94 (MQ=60)
                                                                 CGTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAA                               >  SRR4302542.5521603/1‑94 (MQ=60)
                                                                  GTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                              >  SRR4302542.4491577/1‑94 (MQ=60)
                                                                  GTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                              >  SRR4302542.78131/1‑94 (MQ=60)
                                                                  GTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                              <  SRR4302542.3537581/94‑1 (MQ=60)
                                                                  GTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                              >  SRR4302542.3558051/1‑94 (MQ=60)
                                                                  GTCAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                              <  SRR4302542.4520267/94‑1 (MQ=60)
                                                                    CAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                            <  SRR4302542.1858878/94‑1 (MQ=60)
                                                                    CAGGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                            >  SRR4302542.6526788/1‑94 (MQ=60)
                                                                      GGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                          <  SRR4302542.4727901/94‑1 (MQ=60)
                                                                      GGCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                          <  SRR4302542.2041080/94‑1 (MQ=60)
                                                                      GGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                          <  SRR4302542.3123750/94‑1 (MQ=60)
                                                                       GCGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACA                         <  SRR4302542.4520572/94‑1 (MQ=60)
                                                                       GCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACA                         <  SRR4302542.2799917/94‑1 (MQ=60)
                                                                        CGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                        <  SRR4302542.2425379/94‑1 (MQ=60)
                                                                        CGCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT                        <  SRR4302542.7437529/94‑1 (MQ=60)
                                                                         GCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑C                      >  SRR4302542.442172/1‑94 (MQ=60)
                                                                         GCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑C                      <  SRR4302542.6577403/94‑1 (MQ=60)
                                                                         GCGCGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑C                      <  SRR4302542.6525880/94‑1 (MQ=60)
                                                                         GCGCGGAGATCGACTCCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGGCAATGCCCAGCGCCTCCCCCGGCCATAAATCAAAATAGACAT‑C                      <  SRR4302542.6161829/94‑1 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CG                     <  SRR4302542.1241277/94‑1 (MQ=60)
                                                                            CGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCT                   <  SRR4302542.4269494/94‑1 (MQ=60)
                                                                            CGGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCT                   <  SRR4302542.4269493/94‑1 (MQ=60)
                                                                             GGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTA                  >  SRR4302542.6322665/1‑94 (MQ=60)
                                                                             GGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTA                  >  SRR4302542.1147935/1‑94 (MQ=60)
                                                                             GGAGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTA                  <  SRR4302542.7458270/94‑1 (MQ=60)
                                                                             GGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTA                  >  SRR4302542.1084298/1‑94 (MQ=60)
                                                                               AGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAA                <  SRR4302542.6033174/94‑1 (MQ=60)
                                                                               AGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAA                <  SRR4302542.7019934/94‑1 (MQ=60)
                                                                               AGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAA                <  SRR4302542.720087/94‑1 (MQ=60)
                                                                               AGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAA                <  SRR4302542.7303107/94‑1 (MQ=60)
                                                                               AGATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAA                <  SRR4302542.4936171/94‑1 (MQ=60)
                                                                                NNTCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAG               <  SRR4302542.3753816/94‑1 (MQ=60)
                                                                                GATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAG               >  SRR4302542.5413245/1‑94 (MQ=60)
                                                                                GATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAG               <  SRR4302542.5154636/94‑1 (MQ=60)
                                                                                GATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAG               >  SRR4302542.3535180/1‑94 (MQ=60)
                                                                                GATCGACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAACAAAAAAGACATCCGCTAAA                <  SRR4302542.2375840/94‑1 (MQ=60)
                                                                                   CGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCT            >  SRR4302542.5016532/1‑94 (MQ=60)
                                                                                   CGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCT            <  SRR4302542.1789882/94‑1 (MQ=60)
                                                                                     ACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCCCTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTT          <  SRR4302542.5592350/94‑1 (MQ=60)
                                                                                     ACTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTT          >  SRR4302542.5438039/1‑94 (MQ=60)
                                                                                      CTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTG         <  SRR4302542.6793076/94‑1 (MQ=60)
                                                                                      CTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTG         >  SRR4302542.995296/1‑94 (MQ=60)
                                                                                      CTTCAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTG         <  SRR4302542.3306136/94‑1 (MQ=60)
                                                                                         CAGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTGCCC      >  SRR4302542.4910212/1‑94 (MQ=60)
                                                                                         CAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTGCCC      <  SRR4302542.328151/94‑1 (MQ=60)
                                                                                          AGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTGCCCG     <  SRR4302542.2471659/94‑1 (MQ=60)
                                                                                          AGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTGCCCG     >  SRR4302542.3694262/1‑94 (MQ=60)
                                                                                          AGCGGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAG‑CTTTTCCCGG    >  SRR4302542.1634191/1‑94 (MQ=60)
                                                                                             AGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTGCCCGGCG  >  SRR4302542.2034143/1‑94 (MQ=60)
                                                                                             |                                                                                              
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT‑CGCTAAAGCCTTTGCCCGGCG  >  NC_000913/4316110‑4316296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑