Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,325,494 C→G A261G (GCG→GGG)  yjdA → putative vimentin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,325,4940CG86.4% 744.4 / 94.1 250A261G (GCG→GGG) yjdAputative vimentin
Reads supporting (aligned to +/- strand):  ref base C (14/20);  new base G (102/113);  total (116/134)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-01

Only 100 of 253 total aligned reads displayed.

AGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTGC  >  NC_000913/4325404‑4325583
                                                                                          |                                                                                         
aGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcggggg                                                                                         >  2:4277884/1‑93 (MQ=255)
    gACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACt                                                                                     >  2:3748651/1‑93 (MQ=255)
    gACGTTAATGGATACCCCCGGGCCAAATGAAACCGGGCAACCGCATCTGCAAAAAATGCTTAACAAGCAGCTGGCACGCGCCTCGGGGGAACt                                                                                     >  1:911928/1‑93 (MQ=255)
      cGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTgg                                                                                   <  1:1445347/93‑1 (MQ=255)
       gTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGc                                                                                  <  2:4816234/93‑1 (MQ=255)
        ttACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCg                                                                                 >  2:2036896/1‑93 (MQ=255)
          aCTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGt                                                                               <  1:1213038/93‑1 (MQ=255)
           cTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTg                                                                              >  2:7171207/1‑93 (MQ=255)
             ggATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCt                                                                            >  1:6441163/1‑93 (MQ=255)
              gATCCCCCCGGGCAAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTg                                                                           <  2:6923959/93‑1 (MQ=255)
              gATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTg                                                                           <  2:5531493/93‑1 (MQ=255)
               aTACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTgg                                                                          >  1:2322594/1‑93 (MQ=255)
                tACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGa                                                                         >  2:2843089/1‑93 (MQ=255)
                 aCCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGAt                                                                        <  2:7306867/93‑1 (MQ=255)
                    cccGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATtat                                                                     >  1:1863809/1‑93 (MQ=255)
                    cccGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATtat                                                                     >  1:4782341/1‑93 (MQ=255)
                     ccGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATtata                                                                    <  2:1232092/93‑1 (MQ=255)
                     ccGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATtata                                                                    >  1:76066/1‑93 (MQ=255)
                     ccGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATtata                                                                    >  1:1275029/1‑93 (MQ=255)
                     ccGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATtann                                                                    >  2:1722775/1‑91 (MQ=255)
                      cNGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATtatan                                                                   <  2:6655003/93‑2 (MQ=255)
                      cGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATAc                                                                   >  2:6788496/1‑93 (MQ=255)
                       gggCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACg                                                                  >  1:6761438/1‑93 (MQ=255)
                       gggCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACg                                                                  >  1:1669871/1‑93 (MQ=255)
                       gggCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACg                                                                  >  2:6535898/1‑93 (MQ=255)
                       gggCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACg                                                                  <  1:5104271/93‑1 (MQ=255)
                          ccAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCaa                                                               >  1:2166261/1‑93 (MQ=255)
                           cAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAAc                                                              >  2:4326889/1‑93 (MQ=255)
                            aaaTGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACt                                                             <  2:1821431/93‑1 (MQ=255)
                            aaaTGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACt                                                             >  2:5422645/1‑93 (MQ=255)
                            aaaTGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACt                                                             >  2:6289942/1‑93 (MQ=255)
                             aaTGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTg                                                            >  1:2838766/1‑93 (MQ=255)
                              aTGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGa                                                           <  1:535101/93‑1 (MQ=255)
                               tGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGaa                                                          <  2:3918295/93‑1 (MQ=255)
                               tGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGaa                                                          <  2:3514022/93‑1 (MQ=255)
                                gAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGaaa                                                         <  2:5857803/93‑1 (MQ=255)
                                gAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGaaa                                                         <  2:4863289/93‑1 (MQ=255)
                                      gggCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtc                                                   <  2:867587/93‑1 (MQ=255)
                                      gggCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtc                                                   <  1:1038861/93‑1 (MQ=255)
                                      gggCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtc                                                   <  1:3999178/93‑1 (MQ=255)
                                      gggCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtc                                                   <  1:487560/93‑1 (MQ=255)
                                       ggCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtct                                                  <  2:2208202/93‑1 (MQ=255)
                                        gCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAtctc                                                 <  2:2947208/93‑1 (MQ=255)
                                         cAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTcc                                                >  1:2146047/1‑93 (MQ=255)
                                         cAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGAACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTcc                                                >  1:6809870/1‑93 (MQ=255)
                                          aaCCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCg                                               >  1:3414102/1‑93 (MQ=255)
                                          aaCCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCg                                               <  1:5841072/93‑1 (MQ=255)
                                           nncGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGa                                              <  2:1899523/91‑1 (MQ=255)
                                           aCCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGa                                              >  1:7140406/1‑93 (MQ=255)
                                           aCCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGa                                              >  2:629748/1‑93 (MQ=255)
                                           aCCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGa                                              <  2:4157459/93‑1 (MQ=255)
                                            ccGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGAt                                             <  1:7069878/93‑1 (MQ=255)
                                            ccGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTACACGCAACCGAAATCGATCTCCGAt                                             <  2:7359021/93‑1 (MQ=255)
                                             cGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCACCGATg                                            >  2:7154052/1‑93 (MQ=255)
                                              gCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGa                                           >  1:2215098/1‑93 (MQ=255)
                                               cATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGaa                                          >  2:3065279/1‑93 (MQ=255)
                                               cATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGaa                                          <  2:5050833/93‑1 (MQ=255)
                                               cATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGaa                                          <  1:7332433/93‑1 (MQ=255)
                                                aTCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAag                                         >  2:5829158/1‑93 (MQ=255)
                                                aTCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAag                                         >  2:2900257/1‑93 (MQ=255)
                                                 tCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAaga                                        >  2:5099240/1‑93 (MQ=255)
                                                 tCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACACAACTGAAATCGATCTCCGATGAaga                                        >  2:1653485/1‑93 (MQ=255)
                                                  cTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAagag                                       >  1:2487238/1‑93 (MQ=255)
                                                   tGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTGCTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAgg                                      <  2:1134474/93‑1 (MQ=255)
                                                   tGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAgg                                      >  2:2584670/1‑93 (MQ=255)
                                                    gCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGt                                     <  1:7223698/93‑1 (MQ=255)
                                                    gCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGt                                     >  2:2095976/1‑93 (MQ=255)
                                                       aaaaaTGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCg                                  <  1:3552893/93‑1 (MQ=255)
                                                          aaTGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGa                               <  2:7461793/93‑1 (MQ=255)
                                                          aaTGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGa                               >  2:5275534/1‑93 (MQ=255)
                                                           aTGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAg                              <  1:4422242/93‑1 (MQ=255)
                                                              cTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCg                           >  2:2693247/1‑93 (MQ=255)
                                                              cTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCg                           <  2:3215824/93‑1 (MQ=255)
                                                                tAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGAt                         >  2:2373809/1‑93 (MQ=255)
                                                                 aaCCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGAtt                        <  2:6727026/93‑1 (MQ=255)
                                                                  aCCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGAttt                       <  2:6016719/93‑1 (MQ=255)
                                                                   ccAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGAtttt                      <  1:1105948/93‑1 (MQ=255)
                                                                    cagcagCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTg                     <  1:5948649/93‑1 (MQ=255)
                                                                      gcagcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGc                   <  1:1594268/93‑1 (MQ=255)
                                                                      gcagcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGc                   >  2:3247381/1‑93 (MQ=255)
                                                                        agcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCgg                 <  1:7348368/93‑1 (MQ=255)
                                                                        agcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCgg                 <  2:4031709/93‑1 (MQ=255)
                                                                        agcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCgg                 <  1:3759804/93‑1 (MQ=255)
                                                                         gcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGt                <  1:6559975/93‑1 (MQ=255)
                                                                         gcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGt                >  2:3829770/1‑93 (MQ=255)
                                                                         gcTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGt                <  1:6375018/93‑1 (MQ=255)
                                                                           tGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTgg              <  1:2997791/93‑1 (MQ=255)
                                                                           tGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTgg              >  1:2004384/1‑93 (MQ=255)
                                                                           tGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTgg              <  1:4340874/93‑1 (MQ=255)
                                                                              cACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGc           <  1:1891088/93‑1 (MQ=255)
                                                                                cgcgCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCaa         <  2:7528976/93‑1 (MQ=255)
                                                                                cgcgCCTCGGGGGTACTGACGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCaa         >  2:5339094/1‑93 (MQ=255)
                                                                                 gcgcCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAAt        <  2:3673470/93‑1 (MQ=255)
                                                                                 gcgcCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAAt        <  1:4375810/93‑1 (MQ=255)
                                                                                  cgcCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATc       <  2:1577646/93‑1 (MQ=255)
                                                                                  cgcCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATc       <  1:3885513/93‑1 (MQ=255)
                                                                                   gcCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCg      <  1:4624434/93‑1 (MQ=255)
                                                                                   gcCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCg      >  2:7008231/1‑93 (MQ=255)
                                                                                    ccTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCgg     <  1:4281291/93‑1 (MQ=255)
                                                                                      tCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTg   >  1:4043610/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTGC  >  NC_000913/4325404‑4325583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTGCCGCT  >  NC_000913/4325401‑4325587
                                                                                             |                                                                                             
GTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGG                                                                                               >  SRR4302542.5884689/1‑94 (MQ=60)
GTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGG                                                                                               >  SRR4302542.4499470/1‑94 (MQ=60)
 TCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGG                                                                                              <  SRR4302542.3717079/94‑1 (MQ=60)
  CAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGG                                                                                             <  SRR4302542.5307247/94‑1 (MQ=60)
   AGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGT                                                                                            <  SRR4302542.7087463/94‑1 (MQ=60)
      TGCCGTTACTGGATCCCCCCGGCCAAAGTGAGGCCGGGCAACGGCATTTGAAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACT                                                                                         <  SRR4302542.3910657/94‑1 (MQ=46)
      TGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACT                                                                                         >  SRR4302542.7194449/1‑94 (MQ=60)
      TGACGTTAATGGATACCCCCGGGCCAAATGAAACCGGGCAACCGCATCTGCAAAAAATGCTTAACAAGCAGCTGGCACGCGCCTCGGGGGAACT                                                                                         >  SRR4302542.919790/1‑94 (MQ=60)
         CGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGC                                                                                      >  SRR4302542.1616629/1‑94 (MQ=60)
         CGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGC                                                                                      <  SRR4302542.1457821/94‑1 (MQ=60)
          GTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCG                                                                                     <  SRR4302542.7325256/94‑1 (MQ=60)
          GTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCG                                                                                     <  SRR4302542.6423585/94‑1 (MQ=60)
          GTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCG                                                                                     >  SRR4302542.3940382/1‑94 (MQ=60)
           TTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGG                                                                                    <  SRR4302542.914514/94‑1 (MQ=60)
            TGCTGGATTCCCCCGGCGCAAATGAAGCCGGCCATCCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGGGCCTCGTTGGTACTGGCGGT                                                                                   <  SRR4302542.6005827/94‑1 (MQ=60)
             ACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTG                                                                                  <  SRR4302542.1223477/94‑1 (MQ=60)
               TGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCT                                                                                >  SRR4302542.4833193/1‑94 (MQ=60)
               TGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCT                                                                                >  SRR4302542.6504602/1‑94 (MQ=60)
                 GATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGG                                                                              >  SRR4302542.2342508/1‑94 (MQ=60)
                    ACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATT                                                                           <  SRR4302542.7512885/94‑1 (MQ=60)
                     CCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTA                                                                          >  SRR4302542.4566311/1‑94 (MQ=60)
                      CCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTAT                                                                         >  SRR4302542.1879930/1‑94 (MQ=60)
                      CCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTAT                                                                         <  SRR4302542.4385081/94‑1 (MQ=60)
                      CCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTAT                                                                         >  SRR4302542.4828960/1‑94 (MQ=60)
                       CCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATA                                                                        >  SRR4302542.76697/1‑94 (MQ=60)
                       CCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATA                                                                        <  SRR4302542.7371044/94‑1 (MQ=60)
                       CCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATA                                                                        >  SRR4302542.1285976/1‑94 (MQ=60)
                       CCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATA                                                                        >  SRR4302542.1720232/1‑94 (MQ=60)
                         CGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACG                                                                      >  SRR4302542.6827946/1‑94 (MQ=60)
                         CGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACG                                                                      >  SRR4302542.1684303/1‑94 (MQ=60)
                          GGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGC                                                                     >  SRR4302542.6514096/1‑94 (MQ=60)
                          GGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGC                                                                     <  SRR4302542.5153854/94‑1 (MQ=60)
                           GGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCA                                                                    <  SRR4302542.2140611/94‑1 (MQ=60)
                            GCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAA                                                                   >  SRR4302542.2184918/1‑94 (MQ=60)
                              CAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACT                                                                 >  SRR4302542.4082058/1‑94 (MQ=60)
                              CAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACT                                                                 <  SRR4302542.6291203/94‑1 (MQ=60)
                               AAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTG                                                                >  SRR4302542.5257439/1‑94 (MQ=60)
                               AAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTG                                                                >  SRR4302542.2866049/1‑94 (MQ=60)
                                 ATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAA                                                              <  SRR4302542.539726/94‑1 (MQ=60)
                                   GAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAAT                                                            <  SRR4302542.7222256/94‑1 (MQ=60)
                                   GAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAAT                                                            <  SRR4302542.2185607/94‑1 (MQ=60)
                                    AAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATC                                                           <  SRR4302542.6777302/94‑1 (MQ=60)
                                     AGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCG                                                          <  SRR4302542.2869162/94‑1 (MQ=60)
                                     AGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCG                                                          >  SRR4302542.4744050/1‑94 (MQ=60)
                                       CCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGAT                                                        >  SRR4302542.2978662/1‑94 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      <  SRR4302542.5111537/94‑1 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      <  SRR4302542.4038622/94‑1 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      <  SRR4302542.1047785/94‑1 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      >  SRR4302542.1227316/1‑94 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      <  SRR4302542.714413/94‑1 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      <  SRR4302542.1206018/94‑1 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      <  SRR4302542.491776/94‑1 (MQ=60)
                                         GGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCT                                                      <  SRR4302542.6510425/94‑1 (MQ=60)
                                           GCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCC                                                    >  SRR4302542.2164553/1‑94 (MQ=60)
                                           GCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCC                                                    <  SRR4302542.7411309/94‑1 (MQ=60)
                                           GCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGAACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCC                                                    >  SRR4302542.6876821/1‑94 (MQ=60)
                                            CAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCG                                                   >  SRR4302542.3446090/1‑94 (MQ=60)
                                             AACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGA                                                  >  SRR4302542.6494878/1‑94 (MQ=60)
                                             AACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGA                                                  <  SRR4302542.5897734/94‑1 (MQ=60)
                                             AACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGA                                                  >  SRR4302542.7210479/1‑94 (MQ=60)
                                             AACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGA                                                  <  SRR4302542.1322976/94‑1 (MQ=60)
                                              ACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGAT                                                 <  SRR4302542.4434999/94‑1 (MQ=60)
                                               CCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATG                                                <  SRR4302542.7139267/94‑1 (MQ=60)
                                                CGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGA                                               >  SRR4302542.2234162/1‑94 (MQ=60)
                                                 GCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAA                                              >  SRR4302542.1897949/1‑94 (MQ=60)
                                                  CATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAG                                             <  SRR4302542.7404263/94‑1 (MQ=60)
                                                    TCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAG                                           <  SRR4302542.4292392/94‑1 (MQ=60)
                                                    TCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAG                                           <  SRR4302542.1882789/94‑1 (MQ=60)
                                                    TCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAG                                           >  SRR4302542.2511373/1‑94 (MQ=60)
                                                      TGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGT                                         >  SRR4302542.5213787/1‑94 (MQ=60)
                                                       GCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTC                                        <  SRR4302542.7294555/94‑1 (MQ=60)
                                                        CAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCC                                       >  SRR4302542.3651393/1‑94 (MQ=60)
                                                          AAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGT                                     <  SRR4302542.3586098/94‑1 (MQ=60)
                                                           AAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTG                                    >  SRR4302542.2014389/1‑94 (MQ=60)
                                                            AAATGCTTACCCAGCAGCTGGCACGCGCCTCGG‑GGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAG                                  <  SRR4302542.6846855/94‑1 (MQ=60)
                                                             AATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAG                                  >  SRR4302542.3935944/1‑94 (MQ=60)
                                                             AATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAG                                  <  SRR4302542.1291530/94‑1 (MQ=60)
                                                             AATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAG                                  >  SRR4302542.591016/1‑94 (MQ=60)
                                                              ATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGG                                 <  SRR4302542.7435743/94‑1 (MQ=60)
                                                              ATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGG                                 <  SRR4302542.2272703/94‑1 (MQ=60)
                                                              ATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGG                                 >  SRR4302542.540839/1‑94 (MQ=60)
                                                              ATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGG                                 <  SRR4302542.4465675/94‑1 (MQ=60)
                                                              ATGCTTAACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGG                                 <  SRR4302542.4183361/94‑1 (MQ=60)
                                                                     ACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTT                          >  SRR4302542.2344751/1‑94 (MQ=60)
                                                                     ACCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTT                          >  SRR4302542.5997831/1‑94 (MQ=60)
                                                                      CCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTG                         >  SRR4302542.488201/1‑94 (MQ=60)
                                                                      CCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTG                         <  SRR4302542.1115405/94‑1 (MQ=60)
                                                                      CCAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTG                         >  SRR4302542.4668573/1‑94 (MQ=60)
                                                                       CAGCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGG                        <  SRR4302542.6006263/94‑1 (MQ=60)
                                                                         GCAGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCG                      <  SRR4302542.1608038/94‑1 (MQ=60)
                                                                           AGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGT                    <  SRR4302542.2180705/94‑1 (MQ=60)
                                                                           AGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGT                    >  SRR4302542.113683/1‑94 (MQ=60)
                                                                           AGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGT                    <  SRR4302542.7420336/94‑1 (MQ=60)
                                                                           AGCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGT                    <  SRR4302542.3797000/94‑1 (MQ=60)
                                                                           AGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGT                    >  SRR4302542.2400185/1‑94 (MQ=60)
                                                                            GCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTG                   <  SRR4302542.6437792/94‑1 (MQ=60)
                                                                            GCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTG                   <  SRR4302542.2631174/94‑1 (MQ=60)
                                                                            GCTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTG                   <  SRR4302542.6624632/94‑1 (MQ=60)
                                                                             CTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGG                  >  SRR4302542.2021691/1‑94 (MQ=60)
                                                                             CTGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGG                  <  SRR4302542.4388887/94‑1 (MQ=60)
                                                                             CTGGCACGCGCCTCGGGGGTACTGGCGGGGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGG                  >  SRR4302542.3731980/1‑94 (MQ=60)
                                                                              TGGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGG                 <  SRR4302542.3026471/94‑1 (MQ=60)
                                                                              TGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGG                 <  SRR4302542.4355419/94‑1 (MQ=60)
                                                                              TGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGG                 <  SRR4302542.4383527/94‑1 (MQ=60)
                                                                               GGCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGG                <  SRR4302542.2219813/94‑1 (MQ=60)
                                                                               GGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGG                >  SRR4302542.1049825/1‑94 (MQ=60)
                                                                                GCACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGC               <  SRR4302542.2147820/94‑1 (MQ=60)
                                                                                 CACGCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCA              <  SRR4302542.1907452/94‑1 (MQ=60)
                                                                                    GCGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATC           <  SRR4302542.4418763/94‑1 (MQ=60)
                                                                                     CGCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCG          <  SRR4302542.1547817/94‑1 (MQ=60)
                                                                                     CGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCG          <  SRR4302542.3923879/94‑1 (MQ=60)
                                                                                      GCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGG         <  SRR4302542.4669619/94‑1 (MQ=60)
                                                                                      GCCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGG         <  SRR4302542.1660042/94‑1 (MQ=60)
                                                                                       CCTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGT        <  SRR4302542.4323403/94‑1 (MQ=60)
                                                                                       CCTCGGGGGTACTGGCGGTGCTAGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGT        <  SRR4302542.3216546/94‑1 (MQ=60)
                                                                                       CCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGT        <  SRR4302542.3041442/94‑1 (MQ=60)
                                                                                        CTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTG       >  SRR4302542.3518578/1‑94 (MQ=60)
                                                                                        CTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTG       >  SRR4302542.6852849/1‑94 (MQ=60)
                                                                                        CTCGGGGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTG       >  SRR4302542.4083508/1‑94 (MQ=60)
                                                                                             GGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTGCCGCT  <  SRR4302542.6106430/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTTTGGCGGTGGGGCAATCGGTGCCGCT  >  NC_000913/4325401‑4325587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑