Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,337,885 A→G V73A (GTC→GCC)  adiA ← biodegradative arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,337,8850AG86.6% 771.4 / 100.4 314V73A (GTC→GCC) adiAbiodegradative arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (16/26);  new base G (132/140);  total (148/166)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.33e-01

Only 100 of 317 total aligned reads displayed.

GATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATC  >  NC_000913/4337793‑4337976
                                                                                            |                                                                                           
gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGa                                                                                             <  2:5002370/93‑1 (MQ=255)
gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGa                                                                                             >  1:3577512/1‑93 (MQ=255)
  tCCATTGCGGCGAGGGCTTTTTCCCTATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCg                                                                                           >  2:6123872/1‑93 (MQ=255)
  tCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCg                                                                                           <  2:7099157/93‑1 (MQ=255)
    cATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGtt                                                                                         <  1:3102720/93‑1 (MQ=255)
     aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGttt                                                                                        <  1:6807640/93‑1 (MQ=255)
     aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGttt                                                                                        <  1:4981647/93‑1 (MQ=255)
     aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGttt                                                                                        <  1:4925576/93‑1 (MQ=255)
      ttGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGtttt                                                                                       >  2:5551412/1‑93 (MQ=255)
      ttGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGtttt                                                                                       >  1:6346517/1‑93 (MQ=255)
      ttGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGtttt                                                                                       >  2:1204504/1‑93 (MQ=255)
      ttGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGtttt                                                                                       >  2:5334756/1‑93 (MQ=255)
         cggcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGAt                                                                                    <  1:6124989/93‑1 (MQ=255)
         cggcgAGGGCTTTTGCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGAt                                                                                    <  2:4381081/93‑1 (MQ=255)
           gcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGt                                                                                  <  2:3706366/93‑1 (MQ=255)
           gcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGt                                                                                  >  1:2175386/1‑93 (MQ=255)
             gAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTc                                                                                <  1:5188520/93‑1 (MQ=255)
              aGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCg                                                                               >  2:4207620/1‑93 (MQ=255)
              aGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                                               <  1:2381680/93‑1 (MQ=255)
               gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGt                                                                              >  2:2383114/1‑93 (MQ=255)
               gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGt                                                                              >  1:1029592/1‑93 (MQ=255)
               gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGt                                                                              >  2:4268086/1‑93 (MQ=255)
               gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGt                                                                              <  1:2979843/93‑1 (MQ=255)
                ggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTc                                                                             <  2:5722068/93‑1 (MQ=255)
                 gCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTcc                                                                            <  1:2793066/93‑1 (MQ=255)
                 gCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTcc                                                                            <  1:5308092/93‑1 (MQ=255)
                   tttttCCCGATCGCCCAACAGGAACACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCgg                                                                          >  2:672300/1‑93 (MQ=255)
                    ttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGa                                                                         <  1:6723137/93‑1 (MQ=255)
                    ttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGa                                                                         >  2:7020038/1‑93 (MQ=255)
                    ttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGa                                                                         <  2:4880626/93‑1 (MQ=255)
                       tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGt                                                                      >  1:5075453/1‑93 (MQ=255)
                       tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGt                                                                      >  1:6406787/1‑93 (MQ=255)
                         ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTc                                                                    <  1:4387125/93‑1 (MQ=255)
                          cGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTcc                                                                   <  2:4668047/93‑1 (MQ=255)
                           gATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCa                                                                  >  2:4295747/1‑93 (MQ=255)
                             tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCAtt                                                                <  2:2739354/93‑1 (MQ=255)
                             tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCAtt                                                                >  1:1821391/1‑93 (MQ=255)
                             tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                                <  1:5969790/93‑1 (MQ=255)
                                cccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                                             <  2:263757/93‑1 (MQ=255)
                                    aCAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                                         >  2:5652606/1‑93 (MQ=255)
                                     cAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                                        <  2:254932/93‑1 (MQ=255)
                                     cAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                                        <  1:2605223/93‑1 (MQ=255)
                                      aGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                                       >  2:1638039/1‑93 (MQ=255)
                                       ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGa                                                      >  2:7375645/1‑93 (MQ=255)
                                        gAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGaa                                                     <  1:903500/93‑1 (MQ=255)
                                        gAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGaa                                                     >  2:5232577/1‑93 (MQ=255)
                                         aaGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAAc                                                    <  1:4466440/93‑1 (MQ=255)
                                          aGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACa                                                   <  2:4123210/93‑1 (MQ=255)
                                          aGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACa                                                   <  1:4610001/93‑1 (MQ=255)
                                          aGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACa                                                   <  2:3154203/93‑1 (MQ=255)
                                          aGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACa                                                   >  1:4057348/1‑93 (MQ=255)
                                           gACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACAt                                                  <  2:221069/93‑1 (MQ=255)
                                             ccGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCa                                                >  1:5711665/1‑93 (MQ=255)
                                              cGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                               <  2:4812067/93‑1 (MQ=255)
                                              cGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                               >  1:1122676/1‑93 (MQ=255)
                                                gCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                                             <  2:1758946/93‑1 (MQ=255)
                                                gCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                                             <  2:723493/93‑1 (MQ=255)
                                                gCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                                             >  2:1330426/1‑93 (MQ=255)
                                                gCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                                             <  2:3808071/93‑1 (MQ=255)
                                                 cACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCa                                            >  1:3703989/1‑93 (MQ=255)
                                                   cGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGt                                          >  1:2723623/1‑93 (MQ=255)
                                                    gTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTc                                         <  2:5668694/93‑1 (MQ=255)
                                                     ttttGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCa                                        >  1:3311206/1‑93 (MQ=255)
                                                     ttttGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCa                                        >  2:3898146/1‑93 (MQ=255)
                                                      tttGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                                       <  2:5847561/93‑1 (MQ=255)
                                                      tttGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                                       <  2:5595816/93‑1 (MQ=255)
                                                         gttgGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATgg                                    <  2:796884/93‑1 (MQ=255)
                                                         gttgGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATgg                                    <  2:2799331/93‑1 (MQ=255)
                                                           tgGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCt                                  <  1:5775831/93‑1 (MQ=255)
                                                            ggCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt                                 <  2:638641/93‑1 (MQ=255)
                                                               gcTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGt                              <  2:4427701/93‑1 (MQ=255)
                                                                cTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt                             >  2:6556314/1‑93 (MQ=255)
                                                                 tCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGttt                            >  1:1985065/1‑93 (MQ=255)
                                                                   aTGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGa                          <  2:4010813/93‑1 (MQ=255)
                                                                    tGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGaa                         <  2:3406287/93‑1 (MQ=255)
                                                                    tGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGaa                         <  1:6496013/93‑1 (MQ=255)
                                                                      aaGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAaga                       <  1:1487339/93‑1 (MQ=255)
                                                                        gCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagaga                     <  1:1657519/93‑1 (MQ=255)
                                                                          ttACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga                   >  1:6877005/1‑93 (MQ=255)
                                                                          ttACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga                   <  1:3953390/93‑1 (MQ=255)
                                                                          ttACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga                   <  2:3256276/93‑1 (MQ=255)
                                                                           tACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGaa                  >  2:6847738/1‑93 (MQ=255)
                                                                           tACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTACATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGaa                  <  2:5471097/93‑1 (MQ=255)
                                                                            aCCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAt                 <  1:5217396/93‑1 (MQ=255)
                                                                            aCCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAt                 <  1:4815808/93‑1 (MQ=255)
                                                                             ccGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATg                <  2:1583682/93‑1 (MQ=255)
                                                                             ccGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATg                <  1:6192271/93‑1 (MQ=255)
                                                                               gATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGc              <  1:4121481/93‑1 (MQ=255)
                                                                               gATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGc              >  2:203801/1‑93 (MQ=255)
                                                                               gATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGc              <  2:6215089/93‑1 (MQ=255)
                                                                               gATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGc              >  2:2505967/1‑93 (MQ=255)
                                                                                aTCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCa             >  2:196757/1‑93 (MQ=255)
                                                                                aTCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCa             >  1:7350390/1‑93 (MQ=255)
                                                                                 tCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCaa            >  1:5868252/1‑93 (MQ=255)
                                                                                 tCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCaa            >  2:1027413/1‑93 (MQ=255)
                                                                                 tCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCaa            >  2:2117561/1‑93 (MQ=255)
                                                                                  cAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCaaa           >  1:7487533/1‑93 (MQ=255)
                                                                                   aaTTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCaaaa          >  2:3542756/1‑93 (MQ=255)
                                                                                      tGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACca       >  2:2983062/1‑93 (MQ=255)
                                                                                          tGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACcatcat   <  1:1298001/93‑1 (MQ=255)
                                                                                            |                                                                                           
GATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATC  >  NC_000913/4337793‑4337976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATCAA  >  NC_000913/4337792‑4337978
                                                                                             |                                                                                             
CGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGG                                                                                               >  SRR4302542.4440373/1‑94 (MQ=60)
CGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGG                                                                                               <  SRR4302542.7028342/94‑1 (MQ=60)
CGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGA                                                                                               >  SRR4302542.3610895/1‑94 (MQ=60)
 GATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGC                                                                                              <  SRR4302542.4484889/94‑1 (MQ=60)
 GATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGC                                                                                              <  SRR4302542.6854899/94‑1 (MQ=60)
  ATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCG                                                                                             >  SRR4302542.3572712/1‑94 (MQ=60)
  ATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCG                                                                                             <  SRR4302542.5854799/94‑1 (MQ=60)
   TCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGT                                                                                            >  SRR4302542.1082500/1‑94 (MQ=60)
    CCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTT                                                                                           <  SRR4302542.5873697/94‑1 (MQ=60)
     CATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTT                                                                                          <  SRR4302542.3132203/94‑1 (MQ=60)
      ATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTT                                                                                         <  SRR4302542.6167592/94‑1 (MQ=60)
      ATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTT                                                                                         <  SRR4302542.4973457/94‑1 (MQ=60)
      ATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTT                                                                                         <  SRR4302542.5029999/94‑1 (MQ=60)
      ATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTT                                                                                         <  SRR4302542.6874573/94‑1 (MQ=60)
      ATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTT                                                                                         >  SRR4302542.6409045/1‑94 (MQ=60)
      ATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTT                                                                                         >  SRR4302542.448801/1‑94 (MQ=60)
      ATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTT                                                                                         <  SRR4302542.5843348/94‑1 (MQ=60)
         GCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGAT                                                                                      <  SRR4302542.2418128/94‑1 (MQ=60)
          CGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATG                                                                                     >  SRR4302542.4200477/1‑94 (MQ=60)
          CGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATG                                                                                     <  SRR4302542.6184256/94‑1 (MQ=60)
           GGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGT                                                                                    >  SRR4302542.5217598/1‑94 (MQ=60)
           GGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGT                                                                                    >  SRR4302542.2194112/1‑94 (MQ=60)
            GCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTT                                                                                   <  SRR4302542.3101971/94‑1 (MQ=60)
             CGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTC                                                                                  <  SRR4302542.6101874/94‑1 (MQ=60)
              GAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCG                                                                                 <  SRR4302542.2790283/94‑1 (MQ=60)
              GAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCG                                                                                 <  SRR4302542.5238931/94‑1 (MQ=60)
              GAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCG                                                                                 <  SRR4302542.2133156/94‑1 (MQ=60)
               AGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGT                                                                                >  SRR4302542.1038461/1‑94 (MQ=60)
               AGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGT                                                                                >  SRR4302542.1361011/1‑94 (MQ=60)
               AGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGT                                                                                <  SRR4302542.2403447/94‑1 (MQ=60)
                GGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTC                                                                               <  SRR4302542.3008375/94‑1 (MQ=60)
                 GGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCC                                                                              <  SRR4302542.2380756/94‑1 (MQ=60)
                  GCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCG                                                                             >  SRR4302542.2749502/1‑94 (MQ=60)
                  GCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCG                                                                             >  SRR4302542.1199321/1‑94 (MQ=60)
                  GCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCG                                                                             <  SRR4302542.2819945/94‑1 (MQ=60)
                  GCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCG                                                                             <  SRR4302542.5359660/94‑1 (MQ=60)
                   CTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGG                                                                            <  SRR4302542.6857796/94‑1 (MQ=60)
                    TTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGA                                                                           >  SRR4302542.5723866/1‑94 (MQ=60)
                     TTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGAT                                                                          <  SRR4302542.6789337/94‑1 (MQ=60)
                      TTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATG                                                                         <  SRR4302542.2837366/94‑1 (MQ=60)
                       TTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGT                                                                        >  SRR4302542.6469914/1‑94 (MQ=60)
                       TTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGT                                                                        >  SRR4302542.5124774/1‑94 (MQ=60)
                       TTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGT                                                                        >  SRR4302542.4010401/1‑94 (MQ=60)
                        TCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTT                                                                       <  SRR4302542.4127194/94‑1 (MQ=60)
                         CCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTC                                                                      <  SRR4302542.6215714/94‑1 (MQ=60)
                         CCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTC                                                                      >  SRR4302542.4043814/1‑94 (MQ=60)
                          CCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCC                                                                     <  SRR4302542.4990/94‑1 (MQ=60)
                          CCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCC                                                                     >  SRR4302542.5010885/1‑94 (MQ=60)
                          CCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCC                                                                     <  SRR4302542.4430166/94‑1 (MQ=60)
                          CCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCC                                                                     >  SRR4302542.3810690/1‑94 (MQ=60)
                           CGATCGCCAAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCA                                                                    <  SRR4302542.4377157/94‑1 (MQ=60)
                            GATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCAT                                                                   >  SRR4302542.4644643/1‑94 (MQ=60)
                            GATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCAT                                                                   >  SRR4302542.6478262/1‑94 (MQ=60)
                             ATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATT                                                                  <  SRR4302542.4724850/94‑1 (MQ=60)
                             ATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATT                                                                  >  SRR4302542.1837164/1‑94 (MQ=60)
                              TCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTT                                                                 <  SRR4302542.6027571/94‑1 (MQ=60)
                               CGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTG                                                                <  SRR4302542.7232313/94‑1 (MQ=60)
                                GCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGA                                                               >  SRR4302542.1714874/1‑94 (MQ=60)
                                 CCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGAT                                                              >  SRR4302542.2305062/1‑94 (MQ=60)
                                 CCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGAT                                                              <  SRR4302542.4478960/94‑1 (MQ=60)
                                   CAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAG                                                            >  SRR4302542.4804457/1‑94 (MQ=60)
                                   CAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAG                                                            <  SRR4302542.2572204/94‑1 (MQ=60)
                                    AACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGC                                                           >  SRR4302542.5251202/1‑94 (MQ=60)
                                     ACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCT                                                          >  SRR4302542.163216/1‑94 (MQ=60)
                                     ACAGGAAGACCGGCACGTTTTGTTAGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCT                                                          <  SRR4302542.5058205/94‑1 (MQ=60)
                                      CAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTG                                                         >  SRR4302542.1003722/1‑94 (MQ=60)
                                      CAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTG                                                         <  SRR4302542.5976282/94‑1 (MQ=60)
                                      CAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTG                                                         <  SRR4302542.2630417/94‑1 (MQ=60)
                                       AGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGA                                                        >  SRR4302542.2977759/1‑94 (MQ=60)
                                         GAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAAC                                                      <  SRR4302542.911311/94‑1 (MQ=60)
                                          AAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACA                                                     >  SRR4302542.4097365/1‑94 (MQ=60)
                                          AAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACA                                                     <  SRR4302542.4510245/94‑1 (MQ=60)
                                           AGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACAT                                                    <  SRR4302542.4655053/94‑1 (MQ=60)
                                            GACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATC                                                   >  SRR4302542.1305133/1‑94 (MQ=60)
                                             ACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCA                                                  >  SRR4302542.5767103/1‑94 (MQ=60)
                                              CCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAG                                                 <  SRR4302542.15111/94‑1 (MQ=60)
                                              CCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAG                                                 >  SRR4302542.1132266/1‑94 (MQ=60)
                                              CCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAG                                                 <  SRR4302542.5992328/94‑1 (MQ=60)
                                               CGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGG                                                >  SRR4302542.5283654/1‑94 (MQ=60)
                                                GGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGC                                               <  SRR4302542.6411352/94‑1 (MQ=60)
                                                 GCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCA                                              <  SRR4302542.2799644/94‑1 (MQ=60)
                                                 GCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCA                                              >  SRR4302542.3740638/1‑94 (MQ=60)
                                                  CACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAG                                             >  SRR4302542.2735530/1‑94 (MQ=60)
                                                   ACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGT                                            <  SRR4302542.6267432/94‑1 (MQ=60)
                                                   ACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGT                                            >  SRR4302542.2749902/1‑94 (MQ=60)
                                                    CGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTC                                           >  SRR4302542.5322724/1‑94 (MQ=60)
                                                     GTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCA                                          >  SRR4302542.3342419/1‑94 (MQ=60)
                                                     GTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCA                                          >  SRR4302542.5063798/1‑94 (MQ=60)
                                                      TTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAA                                         <  SRR4302542.4927793/94‑1 (MQ=60)
                                                       TTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAAT                                        <  SRR4302542.657704/94‑1 (MQ=60)
                                                       TTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAAT                                        <  SRR4302542.5198702/94‑1 (MQ=60)
                                                       TTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAAT                                        >  SRR4302542.1061987/1‑94 (MQ=60)
                                                        TTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATG                                       <  SRR4302542.1887118/94‑1 (MQ=60)
                                                         TGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGG                                      <  SRR4302542.5830122/94‑1 (MQ=60)
                                                          GTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGC                                     >  SRR4302542.6201084/1‑94 (MQ=60)
                                                           TTGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCT                                    <  SRR4302542.7591126/94‑1 (MQ=60)
                                                            TGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTT                                   <  SRR4302542.5831898/94‑1 (MQ=60)
                                                            TGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTT                                   >  SRR4302542.850382/1‑94 (MQ=60)
                                                            TGGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTT                                   <  SRR4302542.915139/94‑1 (MQ=60)
                                                             GGCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTC                                  <  SRR4302542.7544315/94‑1 (MQ=60)
                                                              TCGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCG                                 >  SRR4302542.3709550/1‑94 (MQ=60)
                                                               CGCTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTtgg                                >  SRR4302542.6291114/1‑91 (MQ=60)
                                                               CGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGT                                >  SRR4302542.3080020/1‑94 (MQ=60)
                                                                 CTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTT                              >  SRR4302542.1479227/1‑94 (MQ=60)
                                                                 CTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTT                              >  SRR4302542.2002197/1‑94 (MQ=60)
                                                                 CTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTT                              >  SRR4302542.3699005/1‑94 (MQ=60)
                                                                 CTCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTT                              >  SRR4302542.3388908/1‑94 (MQ=60)
                                                                  TCATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTG                             <  SRR4302542.3255298/94‑1 (MQ=60)
                                                                    ATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAA                           <  SRR4302542.5779655/94‑1 (MQ=60)
                                                                    ATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAA                           <  SRR4302542.6932981/94‑1 (MQ=60)
                                                                    ATGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAA                           >  SRR4302542.3308304/1‑94 (MQ=60)
                                                                     TGAAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAG                          <  SRR4302542.6560029/94‑1 (MQ=60)
                                                                       AAGCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAG                        <  SRR4302542.1500202/94‑1 (MQ=60)
                                                                         GCTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAG                      <  SRR4302542.1671844/94‑1 (MQ=60)
                                                                          CTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGA                     <  SRR4302542.7459187/94‑1 (MQ=60)
                                                                          CTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGA                     >  SRR4302542.7349828/1‑94 (MQ=60)
                                                                          CTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGA                     >  SRR4302542.2998876/1‑94 (MQ=60)
                                                                          CTTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGA                     >  SRR4302542.6944553/1‑94 (MQ=60)
                                                                           TTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAA                    >  SRR4302542.5251320/1‑94 (MQ=60)
                                                                           TTACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAA                    <  SRR4302542.3992423/94‑1 (MQ=60)
                                                                            TACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAT                   <  SRR4302542.1954854/94‑1 (MQ=60)
                                                                            TACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAT                   >  SRR4302542.6949280/1‑94 (MQ=60)
                                                                            TACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAT                   >  SRR4302542.1808309/1‑94 (MQ=60)
                                                                             ACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATG                  <  SRR4302542.5268132/94‑1 (MQ=60)
                                                                             ACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATG                  <  SRR4302542.6423454/94‑1 (MQ=60)
                                                                             ACCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATG                  <  SRR4302542.4862741/94‑1 (MQ=60)
                                                                             ACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATG                  <  SRR4302542.4087075/94‑1 (MQ=60)
                                                                              CCGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGG                 <  SRR4302542.6252530/94‑1 (MQ=60)
                                                                               CGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGC                <  SRR4302542.4646539/94‑1 (MQ=60)
                                                                               CGATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGC                >  SRR4302542.4108602/1‑94 (MQ=60)
                                                                                GATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCA               >  SRR4302542.7422372/1‑94 (MQ=60)
                                                                                GATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCA               <  SRR4302542.4162090/94‑1 (MQ=60)
                                                                                 ATCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAA              >  SRR4302542.5925139/1‑94 (MQ=60)
                                                                                 ATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAA              >  SRR4302542.5416846/1‑94 (MQ=60)
                                                                                  TCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAA             >  SRR4302542.7560964/1‑94 (MQ=60)
                                                                                  TCAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAA             >  SRR4302542.6526505/1‑94 (MQ=60)
                                                                                   CAATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAA            >  SRR4302542.6822950/1‑94 (MQ=60)
                                                                                   CAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAA            >  SRR4302542.49364/1‑94 (MQ=60)
                                                                                    AATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAAC           <  SRR4302542.5551358/94‑1 (MQ=60)
                                                                                     ATTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACC          <  SRR4302542.6665809/94‑1 (MQ=60)
                                                                                      TTGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCA         >  SRR4302542.3754297/1‑94 (MQ=60)
                                                                                       TGTCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCAT        >  SRR4302542.6910523/1‑94 (MQ=60)
                                                                                         TCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCA      <  SRR4302542.2062573/94‑1 (MQ=60)
                                                                                         TCTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCA      >  SRR4302542.4938840/1‑94 (MQ=60)
                                                                                          CTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCAT     >  SRR4302542.2829038/1‑94 (MQ=60)
                                                                                          CTGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCAT     >  SRR4302542.2248749/1‑94 (MQ=60)
                                                                                          CTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCAT     <  SRR4302542.1893809/94‑1 (MQ=60)
                                                                                           TGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATC    <  SRR4302542.4122505/94‑1 (MQ=60)
                                                                                           TGGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATC    <  SRR4302542.2944761/94‑1 (MQ=60)
                                                                                           TGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATC    <  SRR4302542.1309177/94‑1 (MQ=60)
                                                                                            GGCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATCA   >  SRR4302542.4780957/1‑94 (MQ=60)
                                                                                            GACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATCA   <  SRR4302542.634793/94‑1 (MQ=60)
                                                                                             GCGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATCAA  >  SRR4302542.2668654/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAATGGCAAAACCATCATCAA  >  NC_000913/4337792‑4337978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑