Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,402,992	A→G	N243S (AAC→AGC)	purA →	adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,402,992	0	A	G	79.8%	537.1 / 137.4	269	N243S (AAC→AGC)	purA	adenylosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base A (27/27); new base G (98/116); total (126/143)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

CGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTG  >  NC_000913/4402901‑4403084

cgTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAac                                                                                             >  2:5742003/1‑93 (MQ=255)

 gTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAgca                                                                                            <  1:368983/93‑1 (MQ=255)

  tCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAgcac                                                                                           >  1:7528919/1‑93 (MQ=255)

    aGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGcacca                                                                                         <  2:1320016/93‑1 (MQ=255)

    aGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGcacca                                                                                         <  1:155936/93‑1 (MQ=255)

    aGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGcacca                                                                                         <  2:3149656/93‑1 (MQ=255)

    aGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGcacca                                                                                         <  1:5958661/93‑1 (MQ=255)

     gCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGcaccac                                                                                        >  1:6264629/1‑93 (MQ=255)

     gCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGcaccac                                                                                        <  1:2546991/93‑1 (MQ=255)

     gCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAAcaccac                                                                                        >  2:1438096/1‑93 (MQ=255)

      cGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTCCTTCCAGCACCAct                                                                                       <  2:6448065/93‑1 (MQ=255)

       gTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCActg                                                                                      <  2:6061568/93‑1 (MQ=255)

        tGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCActgc                                                                                     >  2:6292470/1‑93 (MQ=255)

         ggCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCActgct                                                                                    <  2:1156950/93‑1 (MQ=255)

           cGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCtgg                                                                                  <  2:775690/93‑1 (MQ=255)

            gATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCtggt                                                                                 >  2:4814894/1‑93 (MQ=255)

            gATCTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCtggt                                                                                 <  1:1011070/93‑1 (MQ=255)

             aTTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCtggtg                                                                                <  2:3962462/93‑1 (MQ=255)

             aTTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCtggtg                                                                                >  1:1033341/1‑93 (MQ=255)

              tttCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCtggtgg                                                                               <  1:3807748/93‑1 (MQ=255)

              tttCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCtggtgg                                                                               <  1:3381814/93‑1 (MQ=255)

              tttCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCtggtgg                                                                               >  2:5962418/1‑93 (MQ=255)

               ttCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGgtggc                                                                              <  2:363327/93‑1 (MQ=255)

                 cGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGgtggcgt                                                                            <  2:1661402/93‑1 (MQ=255)

                   tCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGgtggcgtgg                                                                          <  2:6688092/93‑1 (MQ=255)

                     aTGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGtggcgtggcg                                                                        <  2:2945198/93‑1 (MQ=255)

                     aTGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGtggcgtggcg                                                                        >  2:4711922/1‑93 (MQ=255)

                     aTGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGtggcgtggcg                                                                        >  1:3186158/1‑93 (MQ=255)

                      tGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGa                                                                       >  2:5289939/1‑93 (MQ=255)

                        tttGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGAcc                                                                     >  1:3616831/1‑93 (MQ=255)

                         ttGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCg                                                                    >  2:2128220/1‑93 (MQ=255)

                         ttGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCg                                                                    >  1:1120798/1‑93 (MQ=255)

                          tGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCgg                                                                   <  2:5616545/93‑1 (MQ=255)

                            aaGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGtt                                                                 <  2:4345440/93‑1 (MQ=255)

                             aGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTc                                                                <  1:5421826/93‑1 (MQ=255)

                             aGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTc                                                                >  1:2722394/1‑93 (MQ=255)

                              ggTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTcc                                                               <  1:5323376/93‑1 (MQ=255)

                                tGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCgg                                                             <  2:2558195/93‑1 (MQ=255)

                                  cgcAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGcc                                                           <  1:7025961/93‑1 (MQ=255)

                                   gcAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCt                                                          <  2:2003107/93‑1 (MQ=255)

                                    cAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTg                                                         <  1:6607580/93‑1 (MQ=255)

                                     aGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTgg                                                        >  2:6237839/1‑93 (MQ=255)

                                     aGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTgg                                                        <  2:7347835/93‑1 (MQ=255)

                                       ggTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGc                                                      >  1:2384836/1‑93 (MQ=255)

                                        gTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGcc                                                     >  2:3410861/1‑93 (MQ=255)

                                         tACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGccc                                                    <  2:4208065/93‑1 (MQ=255)

                                         tACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGccc                                                    <  2:7414109/93‑1 (MQ=255)

                                          aCGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCcg                                                   >  2:1572536/1‑93 (MQ=255)

                                           cGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCcgc                                                  <  2:2470956/93‑1 (MQ=255)

                                            gctgctGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCcgcg                                                 >  1:4833387/1‑93 (MQ=255)

                                            gctgctGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCcgcg                                                 >  1:1273364/1‑93 (MQ=255)

                                             ctgctgGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGt                                                >  1:28947/1‑93 (MQ=255)

                                              tgctgGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGtt                                               >  1:490863/1‑93 (MQ=255)

                                              tgctgGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGtt                                               >  1:4369204/1‑93 (MQ=255)

                                               gctgGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTa                                              <  2:4080415/93‑1 (MQ=255)

                                                   gATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGtt                                          <  2:4137773/93‑1 (MQ=255)

                                                   gATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGtt                                          <  1:1948424/93‑1 (MQ=255)

                                                     tatCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGa                                        <  1:5626087/93‑1 (MQ=255)

                                                     tatCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGa                                        <  1:4293961/93‑1 (MQ=255)

                                                     tatCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGa                                        <  1:2876308/93‑1 (MQ=255)

                                                     tatCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGa                                        >  1:4580816/1‑93 (MQ=255)

                                                     tatCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGAACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGa                                        >  1:6224472/1‑93 (MQ=255)

                                                      atCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAt                                       >  1:6873098/1‑93 (MQ=255)

                                                       tCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAtt                                      >  2:1561508/1‑93 (MQ=255)

                                                        cGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTa                                     <  1:3299699/93‑1 (MQ=255)

                                                          aCCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACg                                   >  2:4502187/1‑93 (MQ=255)

                                                          aCCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACg                                   <  1:1699074/93‑1 (MQ=255)

                                                          aCCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACg                                   >  2:2083737/1‑93 (MQ=255)

                                                          aCCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACg                                   <  1:3126283/93‑1 (MQ=255)

                                                            cACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGtt                                 <  1:5772369/93‑1 (MQ=255)

                                                            cACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGtt                                 <  2:405269/93‑1 (MQ=255)

                                                             aCGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTc                                >  1:4479935/1‑93 (MQ=255)

                                                              cGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCt                               <  1:2440643/93‑1 (MQ=255)

                                                              cGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCt                               >  1:324380/1‑93 (MQ=255)

                                                              cGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCt                               <  2:5013428/93‑1 (MQ=255)

                                                                gTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTgg                             >  2:1931061/1‑93 (MQ=255)

                                                                gTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTgg                             >  1:5515593/1‑93 (MQ=255)

                                                                   cTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTa                          >  1:6950742/1‑93 (MQ=255)

                                                                   cTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTa                          >  2:6559238/1‑93 (MQ=255)

                                                                   cTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTa                          <  2:4969276/93‑1 (MQ=255)

                                                                    ttATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTAt                         <  2:3208122/93‑1 (MQ=255)

                                                                    ttATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTAt                         >  1:5692437/1‑93 (MQ=255)

                                                                     tATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATc                        >  1:6458765/1‑93 (MQ=255)

                                                                         cgtacgtaACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCa                    >  1:292388/1‑93 (MQ=255)

                                                                         cgtacgtaACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCa                    <  2:6778835/93‑1 (MQ=255)

                                                                           tacgtaACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCaaa                  <  1:3583143/93‑1 (MQ=255)

                                                                               taACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCtt              >  1:6040423/1‑93 (MQ=255)

                                                                               taACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCtt              >  2:7257081/1‑93 (MQ=255)

                                                                               taACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCtt              <  2:1103217/93‑1 (MQ=255)

                                                                                aaCTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTa             <  2:4604160/93‑1 (MQ=255)

                                                                                aaCTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTa             <  1:3978068/93‑1 (MQ=255)

                                                                                 aCTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTAc            <  1:4753556/93‑1 (MQ=255)

                                                                                 aCTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTAc            <  2:5471017/93‑1 (MQ=255)

                                                                                  cttcttCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACt           >  1:1591180/1‑93 (MQ=255)

                                                                                    tcttcCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTcc         >  2:6999398/1‑93 (MQ=255)

                                                                                      ttcCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCAc       <  2:2264416/93‑1 (MQ=255)

                                                                                      ttcCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCAc       <  2:5855134/93‑1 (MQ=255)

                                                                                      ttcCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCAc       <  2:6379121/93‑1 (MQ=255)

                                                                                      ttcCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCAc       >  1:5797694/1‑93 (MQ=255)

                                                                                        ccAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTc     <  1:7119920/93‑1 (MQ=255)

CGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTG  >  NC_000913/4402901‑4403084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGT  >  NC_000913/4402899‑4403085

CGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAG                                                                                               >  SRR4302542.412665/1‑94 (MQ=60)

CGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAG                                                                                               <  SRR4302542.1363257/94‑1 (MQ=60)

CGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAG                                                                                               >  SRR4302542.1686118/1‑94 (MQ=60)

 GCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGC                                                                                              <  SRR4302542.7172504/94‑1 (MQ=60)

   GTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCAC                                                                                            >  SRR4302542.7602937/1‑94 (MQ=60)

   GTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCAC                                                                                            <  SRR4302542.372195/94‑1 (MQ=60)

      AGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCAC                                                                                         <  SRR4302542.6016360/94‑1 (MQ=60)

      AGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCAC                                                                                         >  SRR4302542.6326359/1‑94 (MQ=60)

      AGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCAC                                                                                         <  SRR4302542.157259/94‑1 (MQ=60)

       GCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACT                                                                                        <  SRR4302542.2571672/94‑1 (MQ=60)

        CGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTG                                                                                       <  SRR4302542.5464853/94‑1 (MQ=60)

         GTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGC                                                                                      >  SRR4302542.2905098/1‑94 (MQ=60)

          TGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCT                                                                                     <  SRR4302542.5661998/94‑1 (MQ=60)

            GCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGG                                                                                   >  SRR4302542.312095/1‑94 (MQ=60)

             CGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGT                                                                                  <  SRR4302542.2798298/94‑1 (MQ=60)

              GATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTG                                                                                 <  SRR4302542.5253228/94‑1 (MQ=60)

              GATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTG                                                                                 >  SRR4302542.1042227/1‑94 (MQ=60)

              GATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTG                                                                                 >  SRR4302542.3361266/1‑94 (MQ=60)

              GATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTG                                                                                 <  SRR4302542.2927713/94‑1 (MQ=60)

              GATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTG                                                                                 <  SRR4302542.2932198/94‑1 (MQ=60)

              GATCTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTG                                                                                 <  SRR4302542.1019778/94‑1 (MQ=60)

                TTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGC                                                                               <  SRR4302542.3845428/94‑1 (MQ=60)

                TTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGC                                                                               <  SRR4302542.3413579/94‑1 (MQ=60)

                TTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGC                                                                               >  SRR4302542.3169587/1‑94 (MQ=60)

                   CGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTG                                                                            <  SRR4302542.3706419/94‑1 (MQ=60)

                   CGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTG                                                                            <  SRR4302542.6409717/94‑1 (MQ=60)

                   CGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTG                                                                            <  SRR4302542.557452/94‑1 (MQ=60)

                    GTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGG                                                                           >  SRR4302542.4373467/1‑94 (MQ=60)

                    GTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGG                                                                           <  SRR4302542.577053/94‑1 (MQ=60)

                    GTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGG                                                                           >  SRR4302542.3561869/1‑94 (MQ=60)

                    GTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGG                                                                           >  SRR4302542.6092158/1‑94 (MQ=60)

                      CATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCG                                                                         >  SRR4302542.3216340/1‑94 (MQ=60)

                       ATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGA                                                                        >  SRR4302542.3979379/1‑94 (MQ=60)

                       ATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGA                                                                        >  SRR4302542.1021041/1‑94 (MQ=60)

                        TGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGAC                                                                       >  SRR4302542.6212383/1‑94 (MQ=60)

                        TGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGAC                                                                       >  SRR4302542.4790527/1‑94 (MQ=60)

                         GTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACC                                                                      >  SRR4302542.3650533/1‑94 (MQ=60)

                         GTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACC                                                                      >  SRR4302542.6110917/1‑94 (MQ=60)

                          TTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCG                                                                     <  SRR4302542.3688928/94‑1 (MQ=60)

                          TTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCG                                                                     <  SRR4302542.3197179/94‑1 (MQ=60)

                          TTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCG                                                                     >  SRR4302542.1130377/1‑94 (MQ=60)

                           TTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGG                                                                    <  SRR4302542.2513665/94‑1 (MQ=60)

                            TGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGT                                                                   >  SRR4302542.1110956/1‑94 (MQ=60)

                            TGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGT                                                                   <  SRR4302542.5396898/94‑1 (MQ=60)

                              AAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTC                                                                 >  SRR4302542.2748642/1‑94 (MQ=60)

                               AGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCC                                                                <  SRR4302542.5939044/94‑1 (MQ=60)

                               AGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCC                                                                <  SRR4302542.5474469/94‑1 (MQ=60)

                                GGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCG                                                               <  SRR4302542.5375090/94‑1 (MQ=60)

                                GGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCG                                                               <  SRR4302542.2569464/94‑1 (MQ=60)

                                  TGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGC                                                             >  SRR4302542.3727724/1‑94 (MQ=60)

                                    CGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCT                                                           <  SRR4302542.6537392/94‑1 (MQ=60)

                                    CGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCT                                                           >  SRR4302542.6777640/1‑94 (MQ=60)

                                    CGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCT                                                           <  SRR4302542.1441338/94‑1 (MQ=60)

                                    CGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCT                                                           <  SRR4302542.7095007/94‑1 (MQ=60)

                                    CGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCT                                                           <  SRR4302542.2866281/94‑1 (MQ=60)

                                     GCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTG                                                          <  SRR4302542.3362765/94‑1 (MQ=60)

                                      CAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGG                                                         <  SRR4302542.6672672/94‑1 (MQ=60)

                                      CAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGG                                                         >  SRR4302542.3612256/1‑94 (MQ=60)

                                       AGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGG                                                        <  SRR4302542.6089596/94‑1 (MQ=60)

                                       AGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGG                                                        <  SRR4302542.3328006/94‑1 (MQ=60)

                                        GGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGC                                                       >  SRR4302542.2406920/1‑94 (MQ=60)

                                           TACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCG                                                    <  SRR4302542.3272288/94‑1 (MQ=60)

                                           TACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCG                                                    <  SRR4302542.6880576/94‑1 (MQ=60)

                                             CGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCG                                                  >  SRR4302542.6586004/1‑94 (MQ=60)

                                             CGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCG                                                  >  SRR4302542.4880475/1‑94 (MQ=60)

                                             CGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCG                                                  >  SRR4302542.1284301/1‑94 (MQ=60)

                                             CGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCG                                                  >  SRR4302542.3303680/1‑94 (MQ=60)

                                              GCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGT                                                 >  SRR4302542.29204/1‑94 (MQ=60)

                                               CTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTT                                                >  SRR4302542.495102/1‑94 (MQ=60)

                                               CTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTT                                                >  SRR4302542.4412094/1‑94 (MQ=60)

                                                 GCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTAT                                              <  SRR4302542.5853304/94‑1 (MQ=60)

                                                 GCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTAT                                              >  SRR4302542.1897706/1‑94 (MQ=60)

                                                  CTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATG                                             >  SRR4302542.172695/1‑94 (MQ=60)

                                                  CTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATG                                             >  SRR4302542.2054845/1‑94 (MQ=60)

                                                    GGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTT                                           <  SRR4302542.4802498/94‑1 (MQ=60)

                                                    GGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTT                                           <  SRR4302542.7013874/94‑1 (MQ=60)

                                                     GATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTG                                          <  SRR4302542.4435396/94‑1 (MQ=60)

                                                     GATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTG                                          <  SRR4302542.7173165/94‑1 (MQ=60)

                                                     GATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTG                                          <  SRR4302542.1965272/94‑1 (MQ=60)

                                                      ATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGA                                         >  SRR4302542.4528923/1‑94 (MQ=60)

                                                      ATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGA                                         >  SRR4302542.4625629/1‑94 (MQ=60)

                                                      ATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGA                                         >  SRR4302542.4943827/1‑94 (MQ=60)

                                                      ATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGAACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGA                                         >  SRR4302542.6285768/1‑94 (MQ=60)

                                                       TATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAT                                        >  SRR4302542.5700741/1‑94 (MQ=60)

                                                       TATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAT                                        <  SRR4302542.4336195/94‑1 (MQ=60)

                                                       TATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAT                                        <  SRR4302542.5680719/94‑1 (MQ=60)

                                                       TATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAT                                        <  SRR4302542.2903924/94‑1 (MQ=60)

                                                       TATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAT                                        <  SRR4302542.59266/94‑1 (MQ=60)

                                                       TATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAT                                        >  SRR4302542.6940620/1‑94 (MQ=60)

                                                         tggacccggttcctttcCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTA                                      <  SRR4302542.2382472/77‑1 (MQ=60)

                                                          CGACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTAC                                     <  SRR4302542.3330829/94‑1 (MQ=60)

                                                           GACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACG                                    >  SRR4302542.4888769/1‑94 (MQ=60)

                                                            ACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGT                                   <  SRR4302542.262641/94‑1 (MQ=60)

                                                            ACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGT                                   <  SRR4302542.3156017/94‑1 (MQ=60)

                                                            ACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGT                                   <  SRR4302542.2406121/94‑1 (MQ=60)

                                                            ACCACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGT                                   <  SRR4302542.1713795/94‑1 (MQ=60)

                                                              CACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTC                                 <  SRR4302542.5828396/94‑1 (MQ=60)

                                                              CACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTC                                 <  SRR4302542.243495/94‑1 (MQ=60)

                                                              CACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTC                                 <  SRR4302542.3976472/94‑1 (MQ=60)

                                                              CACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTC                                 >  SRR4302542.4523913/1‑94 (MQ=60)

                                                              CACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTC                                 >  SRR4302542.7073346/1‑94 (MQ=60)

                                                              CACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTC                                 <  SRR4302542.4473655/94‑1 (MQ=60)

                                                               ACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCT                                <  SRR4302542.1675865/94‑1 (MQ=60)

                                                               ACGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCT                                >  SRR4302542.327194/1‑94 (MQ=60)

                                                               ACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCT                                <  SRR4302542.5544281/94‑1 (MQ=60)

                                                                CGGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTG                               <  SRR4302542.2464392/94‑1 (MQ=60)

                                                                 GGTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGG                              >  SRR4302542.5569195/1‑94 (MQ=60)

                                                                  GTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGG                             <  SRR4302542.1230664/94‑1 (MQ=60)

                                                                  GTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGG                             >  SRR4302542.5289810/1‑94 (MQ=60)

                                                                  GTACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGG                             <  SRR4302542.1175922/94‑1 (MQ=60)

                                                                    ACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTA                           <  SRR4302542.6290478/94‑1 (MQ=60)

                                                                    ACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTA                           >  SRR4302542.7019034/1‑94 (MQ=60)

                                                                    ACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTA                           >  SRR4302542.958902/1‑94 (MQ=60)

                                                                    ACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTA                           >  SRR4302542.5768038/1‑94 (MQ=60)

                                                                    ACTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTA                           >  SRR4302542.569441/1‑94 (MQ=60)

                                                                     CTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTAT                          >  SRR4302542.1526836/1‑94 (MQ=60)

                                                                     CTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTAT                          >  SRR4302542.5747711/1‑94 (MQ=60)

                                                                     CTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTAT                          >  SRR4302542.3644311/1‑94 (MQ=60)

                                                                     CTTATCCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTAT                          <  SRR4302542.7196758/94‑1 (MQ=60)

                                                                      TTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATC                         <  SRR4302542.7134703/94‑1 (MQ=60)

                                                                      TTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATC                         >  SRR4302542.6522359/1‑94 (MQ=60)

                                                                       TATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCC                        <  SRR4302542.11091/94‑1 (MQ=60)

                                                                          CCGTACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCA                     >  SRR4302542.294940/1‑94 (MQ=60)

                                                                           CGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAA                    >  SRR4302542.4259252/1‑94 (MQ=60)

                                                                            GTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAA                   >  SRR4302542.7086645/1‑94 (MQ=60)

                                                                             TACGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAG                  <  SRR4302542.3616564/94‑1 (MQ=60)

                                                                               CGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCT                >  SRR4302542.846750/1‑94 (MQ=60)

                                                                               CGTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTACCCTCAAAGCT                <  SRR4302542.4679370/94‑1 (MQ=60)

                                                                                GTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTT               >  SRR4302542.1593467/1‑94 (MQ=60)

                                                                                GTAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTT               >  SRR4302542.6098898/1‑94 (MQ=60)

                                                                                 TAACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTA              >  SRR4302542.306399/1‑94 (MQ=60)

                                                                                  AACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTAC             <  SRR4302542.6424578/94‑1 (MQ=60)

                                                                                  AACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTAC             <  SRR4302542.4017319/94‑1 (MQ=60)

                                                                                  AACTTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTAC             >  SRR4302542.4153357/1‑94 (MQ=60)

                                                                                   ACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACT            <  SRR4302542.4799860/94‑1 (MQ=60)

                                                                                   ACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACT            >  SRR4302542.1604928/1‑94 (MQ=60)

                                                                                     TTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCC          <  SRR4302542.1648140/94‑1 (MQ=60)

                                                                                     TTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCC          >  SRR4302542.3820753/1‑94 (MQ=60)

                                                                                     TTCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCC          <  SRR4302542.7430978/94‑1 (MQ=60)

                                                                                      TCTTCCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCA         <  SRR4302542.4585096/94‑1 (MQ=60)

                                                                                       CTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCAC        >  SRR4302542.5853966/1‑94 (MQ=60)

                                                                                         TCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTC      >  SRR4302542.2703015/1‑94 (MQ=60)

                                                                                          CCAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCG     <  SRR4302542.7189804/94‑1 (MQ=60)

                                                                                           CAGCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGT    >  SRR4302542.6543166/1‑94 (MQ=60)

                                                                                            AGCACCACTGCTGGTGGAGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTG   >  SRR4302542.6170675/1‑94 (MQ=60)

                                                                                             GCACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGT  <  SRR4302542.805983/94‑1 (MQ=60)

CGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGT  >  NC_000913/4402899‑4403085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`