Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,456,991 A→G intergenic (+106/‑91) pmbA → / → b4236 PmbA/cytochrome b(562)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,456,9910AG81.1% 640.8 / 147.5 307intergenic (+106/‑91)pmbA/b4236PmbA/cytochrome b(562)
Reads supporting (aligned to +/- strand):  ref base A (20/38);  new base G (124/125);  total (144/163)
Fisher's exact test for biased strand distribution p-value = 4.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 307 total aligned reads displayed.

CTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTC  >  NC_000913/4456901‑4457080
                                                                                          |                                                                                         
cTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCg                                                                                         <  1:3164577/93‑1 (MQ=255)
  gCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTg                                                                                       >  1:2563468/1‑93 (MQ=255)
   ccGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGa                                                                                      >  2:4035929/1‑93 (MQ=255)
      cgcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGAccc                                                                                   <  1:186576/93‑1 (MQ=255)
       gcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCg                                                                                  >  1:1297539/1‑93 (MQ=255)
       gcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCg                                                                                  >  2:3960794/1‑93 (MQ=255)
        cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCgg                                                                                 >  2:2592253/1‑93 (MQ=255)
         tataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGt                                                                                >  2:1902187/1‑93 (MQ=255)
          ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTc                                                                               >  1:882181/1‑93 (MQ=255)
          ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTc                                                                               >  2:6009959/1‑93 (MQ=255)
           taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCg                                                                              >  2:144904/1‑93 (MQ=255)
           taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCg                                                                              >  2:1937496/1‑93 (MQ=255)
             aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGaa                                                                            <  1:1038323/93‑1 (MQ=255)
              aCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAAt                                                                           >  2:2095288/1‑93 (MQ=255)
              aCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAAt                                                                           >  2:3777286/1‑93 (MQ=255)
               cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATg                                                                          >  2:668616/1‑93 (MQ=255)
               cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATg                                                                          >  2:2971364/1‑93 (MQ=255)
                tAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGa                                                                         <  2:3356110/93‑1 (MQ=255)
                tAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGa                                                                         >  1:3286338/1‑93 (MQ=255)
                 aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAg                                                                        <  1:1478972/93‑1 (MQ=255)
                  aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGc                                                                       >  1:6568513/1‑93 (MQ=255)
                  aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGc                                                                       >  1:3007383/1‑93 (MQ=255)
                     aaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTa                                                                    <  1:7307333/93‑1 (MQ=255)
                     aaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTa                                                                    <  2:437535/93‑1 (MQ=255)
                      aTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAg                                                                   >  1:4836829/1‑93 (MQ=255)
                      aTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAg                                                                   <  2:5388706/93‑1 (MQ=255)
                      aTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAg                                                                   >  1:4020531/1‑93 (MQ=255)
                       ttACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAgt                                                                  >  1:4540148/1‑93 (MQ=255)
                       ttACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAgt                                                                  >  2:6289287/1‑93 (MQ=255)
                       ttACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAgt                                                                  >  2:1535569/1‑93 (MQ=255)
                         acacAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAgtcg                                                                <  1:4209360/93‑1 (MQ=255)
                          cacaAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAgtcgt                                                               >  1:2304743/1‑93 (MQ=255)
                            caAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGtcgtcg                                                             >  1:797385/1‑93 (MQ=255)
                              aaaTCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGAt                                                           >  1:3441306/1‑93 (MQ=255)
                               aaTCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATg                                                          <  2:660965/93‑1 (MQ=255)
                                aTCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGa                                                         >  1:2779358/1‑93 (MQ=255)
                                   aTTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGc                                                      <  1:1741496/93‑1 (MQ=255)
                                   aTTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGc                                                      <  1:2154347/93‑1 (MQ=255)
                                   aTTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGc                                                      >  1:5758588/1‑93 (MQ=255)
                                    ttCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCa                                                     <  1:1139264/93‑1 (MQ=255)
                                    ttCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCa                                                     >  1:636689/1‑93 (MQ=255)
                                     tCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAg                                                    <  1:987818/93‑1 (MQ=255)
                                       gCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAgtg                                                  <  2:3210644/93‑1 (MQ=255)
                                       gCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAgtg                                                  >  1:2457683/1‑93 (MQ=255)
                                        cACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAgtgt                                                 <  2:3304810/93‑1 (MQ=255)
                                          ntGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTNa                                               >  2:5210814/2‑93 (MQ=255)
                                           tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGaa                                              >  1:3878240/1‑93 (MQ=255)
                                           tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGaa                                              <  1:4670439/93‑1 (MQ=255)
                                           tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGaa                                              >  2:1685652/1‑93 (MQ=255)
                                           tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGaa                                              >  1:5334473/1‑93 (MQ=255)
                                            gCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAg                                             <  2:3918510/93‑1 (MQ=255)
                                            gCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAg                                             >  2:5887603/1‑93 (MQ=255)
                                             cATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAgg                                            >  2:5692361/1‑93 (MQ=255)
                                              aTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGa                                           <  2:2864400/93‑1 (MQ=255)
                                              aTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGa                                           >  2:252510/1‑93 (MQ=255)
                                              aTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGa                                           >  2:6147136/1‑93 (MQ=255)
                                              aTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGa                                           >  2:1975423/1‑93 (MQ=255)
                                               tCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGAt                                          <  1:3534180/93‑1 (MQ=255)
                                               tCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGAt                                          <  2:494280/93‑1 (MQ=255)
                                               tCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGAt                                          <  1:4804603/93‑1 (MQ=255)
                                               tCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGAt                                          <  1:4136095/93‑1 (MQ=255)
                                                 gagaCGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGCTGa                                        >  2:2394739/1‑93 (MQ=255)
                                                  agaCGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGaa                                       >  2:4743662/1‑93 (MQ=255)
                                                   gaCGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAg                                      <  2:769012/93‑1 (MQ=255)
                                                     cGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAgtg                                    <  2:6049088/93‑1 (MQ=255)
                                                       gAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGta                                  <  1:5156269/93‑1 (MQ=255)
                                                        aaaGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGtaa                                 >  2:7143489/1‑93 (MQ=255)
                                                         aaGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGtaaa                                <  1:5051445/93‑1 (MQ=255)
                                                         aaGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGtaaa                                <  2:6434028/93‑1 (MQ=255)
                                                         aaGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGtaaa                                >  1:2966024/1‑93 (MQ=255)
                                                              aaTGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATaaaa                           <  1:5977817/93‑1 (MQ=255)
                                                               aTGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATaaaaa                          <  1:3760249/93‑1 (MQ=255)
                                                               aTGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATaaaaa                          <  1:779657/93‑1 (MQ=255)
                                                                 gAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAg                        <  1:1823146/93‑1 (MQ=255)
                                                                 gAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAg                        >  1:7124673/1‑93 (MQ=255)
                                                                   gggCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGa                      <  1:6882066/93‑1 (MQ=255)
                                                                   gggCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGa                      >  2:2234106/1‑93 (MQ=255)
                                                                   gggCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGa                      <  1:5268706/93‑1 (MQ=255)
                                                                    ggCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGaa                     <  2:5848909/93‑1 (MQ=255)
                                                                    ggCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGaa                     <  2:5136693/93‑1 (MQ=255)
                                                                      ccGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACt                   <  1:1580773/93‑1 (MQ=255)
                                                                       cGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTa                  >  2:4846399/1‑93 (MQ=255)
                                                                       cGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTa                  <  2:1723680/93‑1 (MQ=255)
                                                                        ggggAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTAt                 >  2:5102752/1‑93 (MQ=255)
                                                                        ggggAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTAt                 <  1:4274086/93‑1 (MQ=255)
                                                                        ggggAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTAt                 <  2:3520626/93‑1 (MQ=255)
                                                                          ggAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTc               <  2:1018026/93‑1 (MQ=255)
                                                                          ggAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTc               >  1:2001487/1‑93 (MQ=255)
                                                                           gAGCGTACTCGCAGTGCGTGACCCGGTCAAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCt              <  2:424601/93‑1 (MQ=255)
                                                                             gCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTg            <  1:5534435/93‑1 (MQ=255)
                                                                             gCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTg            >  1:6809528/1‑93 (MQ=255)
                                                                             gCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTg            >  2:6818852/1‑93 (MQ=255)
                                                                              cGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGc           <  1:7053296/93‑1 (MQ=255)
                                                                              cGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGc           <  2:1126679/93‑1 (MQ=255)
                                                                               gTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCa          >  1:6091004/1‑93 (MQ=255)
                                                                                tACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAg         >  1:2701376/1‑93 (MQ=255)
                                                                                tACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAg         >  1:5636214/1‑93 (MQ=255)
                                                                                    cGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGtctc     >  1:6595394/1‑93 (MQ=255)
                                                                                      cAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTctcct   <  2:3629263/93‑1 (MQ=255)
                                                                                       aGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTctcctc  >  2:5530356/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTC  >  NC_000913/4456901‑4457080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTCGTT  >  NC_000913/4456899‑4457083
                                                                                            |                                                                                            
ACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGC                                                                                             >  SRR4302542.490663/1‑94 (MQ=60)
  CTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGT                                                                                           <  SRR4302542.788172/94‑1 (MQ=60)
  CTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGT                                                                                           <  SRR4302542.3194603/94‑1 (MQ=60)
   TGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTG                                                                                          >  SRR4302542.7183944/1‑94 (MQ=60)
   TGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTG                                                                                          >  SRR4302542.2588320/1‑94 (MQ=60)
       GCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCC                                                                                      >  SRR4302542.3408716/1‑94 (MQ=60)
        CGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCG                                                                                     >  SRR4302542.1308712/1‑94 (MQ=60)
        CGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCG                                                                                     <  SRR4302542.655104/94‑1 (MQ=60)
        CGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCG                                                                                     <  SRR4302542.188160/94‑1 (MQ=60)
        CGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCG                                                                                     <  SRR4302542.302810/94‑1 (MQ=60)
          CTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGT                                                                                   >  SRR4302542.1206317/1‑94 (MQ=60)
          CTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGT                                                                                   >  SRR4302542.7593081/1‑94 (MQ=60)
          CTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGT                                                                                   >  SRR4302542.3963108/1‑94 (MQ=60)
          CTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGG‑CGGGGAGCGTACTCGCAGTGCGTGACCCGGTC                                                                                  <  SRR4302542.1421930/94‑1 (MQ=60)
           TATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTC                                                                                  >  SRR4302542.889803/1‑94 (MQ=60)
            ATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCG                                                                                 >  SRR4302542.3421801/1‑94 (MQ=60)
               AACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAAT                                                                              <  SRR4302542.4730477/94‑1 (MQ=60)
               AACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAAT                                                                              <  SRR4302542.1047242/94‑1 (MQ=60)
                 CTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGA                                                                            >  SRR4302542.4063222/1‑94 (MQ=60)
                 CTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGA                                                                            <  SRR4302542.3520948/94‑1 (MQ=60)
                 CTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGA                                                                            >  SRR4302542.3317368/1‑94 (MQ=60)
                  TAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAG                                                                           <  SRR4302542.2507515/94‑1 (MQ=60)
                   AAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGC                                                                          >  SRR4302542.6633235/1‑94 (MQ=60)
                   AAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGC                                                                          <  SRR4302542.5190980/94‑1 (MQ=60)
                   AAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGC                                                                          >  SRR4302542.3036137/1‑94 (MQ=60)
                   AAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGC                                                                          <  SRR4302542.1491732/94‑1 (MQ=60)
                   AAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGC                                                                          <  SRR4302542.221968/94‑1 (MQ=60)
                    AAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCG                                                                         <  SRR4302542.2879566/94‑1 (MQ=60)
                      AAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTA                                                                       >  SRR4302542.7263389/1‑94 (MQ=60)
                      AAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTA                                                                       <  SRR4302542.1774202/94‑1 (MQ=60)
                       AATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAG                                                                      >  SRR4302542.1497651/1‑94 (MQ=60)
                       AATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAG                                                                      <  SRR4302542.5106586/94‑1 (MQ=60)
                       AATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAG                                                                      >  SRR4302542.4883945/1‑94 (MQ=60)
                       AATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAG                                                                      >  SRR4302542.7566429/1‑94 (MQ=60)
                       AATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAG                                                                      <  SRR4302542.7378913/94‑1 (MQ=60)
                       AATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG                                                                      >  SRR4302542.4060205/1‑94 (MQ=60)
                        ATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGT                                                                     >  SRR4302542.4584597/1‑94 (MQ=60)
                          TACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCG                                                                   >  SRR4302542.4304295/1‑94 (MQ=60)
                           ACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGT                                                                  >  SRR4302542.6019013/1‑94 (MQ=60)
                           ACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGT                                                                  >  SRR4302542.2324539/1‑94 (MQ=60)
                           ACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGT                                                                  <  SRR4302542.4250853/94‑1 (MQ=60)
                            CACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTC                                                                 >  SRR4302542.605186/1‑94 (MQ=60)
                             ACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCG                                                                <  SRR4302542.5727601/94‑1 (MQ=60)
                             ACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCG                                                                >  SRR4302542.804245/1‑94 (MQ=60)
                              CAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGA                                                               <  SRR4302542.2403279/94‑1 (MQ=60)
                               AAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGAT                                                              <  SRR4302542.6784035/94‑1 (MQ=60)
                               AAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGAT                                                              >  SRR4302542.3473523/1‑94 (MQ=60)
                               AAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGAT                                                              <  SRR4302542.5365909/94‑1 (MQ=60)
                                AAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATG                                                             >  SRR4302542.7252354/1‑94 (MQ=60)
                                 AATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGA                                                            >  SRR4302542.6651979/1‑94 (MQ=60)
                                 AATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGA                                                            >  SRR4302542.2806124/1‑94 (MQ=60)
                                  ATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAG                                                           <  SRR4302542.4159749/94‑1 (MQ=60)
                                  ATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAG                                                           <  SRR4302542.3195824/94‑1 (MQ=60)
                                   TCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGG                                                          <  SRR4302542.6899262/94‑1 (MQ=60)
                                    CATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGC                                                         >  SRR4302542.4861711/1‑94 (MQ=60)
                                    CATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGC                                                         >  SRR4302542.1131607/1‑94 (MQ=60)
                                    CATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGC                                                         <  SRR4302542.6999650/94‑1 (MQ=60)
                                    CATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGC                                                         >  SRR4302542.5814484/1‑94 (MQ=60)
                                     ATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCA                                                        <  SRR4302542.1756604/94‑1 (MQ=60)
                                     ATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCA                                                        <  SRR4302542.2172910/94‑1 (MQ=60)
                                     ATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCA                                                        >  SRR4302542.642166/1‑94 (MQ=60)
                                      TTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAG                                                       <  SRR4302542.1149021/94‑1 (MQ=60)
                                      TTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAG                                                       <  SRR4302542.2831627/94‑1 (MQ=60)
                                      TTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAG                                                       <  SRR4302542.6991225/94‑1 (MQ=60)
                                       TCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGT                                                      <  SRR4302542.996332/94‑1 (MQ=60)
                                       TCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGT                                                      <  SRR4302542.5437954/94‑1 (MQ=60)
                                       TCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGT                                                      <  SRR4302542.4866922/94‑1 (MQ=60)
                                        CGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTG                                                     >  SRR4302542.3210417/1‑94 (MQ=60)
                                        CGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTG                                                     <  SRR4302542.1688858/94‑1 (MQ=60)
                                        CGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTG                                                     <  SRR4302542.195620/94‑1 (MQ=60)
                                        CGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTG                                                     >  SRR4302542.5641870/1‑94 (MQ=60)
                                        CGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTG                                                     <  SRR4302542.2770037/94‑1 (MQ=60)
                                        CGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTG                                                     >  SRR4302542.2481560/1‑94 (MQ=60)
                                         GCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGT                                                    >  SRR4302542.5263107/1‑94 (MQ=60)
                                         GCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGT                                                    <  SRR4302542.3608716/94‑1 (MQ=60)
                                          CACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTG                                                   >  SRR4302542.812950/1‑94 (MQ=60)
                                            CTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAA                                                 <  SRR4302542.4203693/94‑1 (MQ=60)
                                            CTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAA                                                 >  SRR4302542.5386305/1‑94 (MQ=60)
                                            CTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAA                                                 >  SRR4302542.3014352/1‑94 (MQ=60)
                                            CTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAA                                                 >  SRR4302542.6400417/1‑94 (MQ=60)
                                            CTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAA                                                 >  SRR4302542.3916547/1‑94 (MQ=60)
                                            CTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAA                                                 >  SRR4302542.1506337/1‑94 (MQ=60)
                                             TGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAG                                                <  SRR4302542.4716085/94‑1 (MQ=60)
                                             TGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAG                                                <  SRR4302542.128108/94‑1 (MQ=60)
                                             TGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAG                                                <  SRR4302542.6297811/94‑1 (MQ=60)
                                              GCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGG                                               <  SRR4302542.1891865/94‑1 (MQ=60)
                                               CATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGA                                              <  SRR4302542.3813662/94‑1 (MQ=60)
                                               CATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGA                                              <  SRR4302542.2045412/94‑1 (MQ=60)
                                                ATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGAT                                             <  SRR4302542.4978409/94‑1 (MQ=60)
                                                 TCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATG                                            <  SRR4302542.4755322/94‑1 (MQ=60)
                                                 TCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATG                                            <  SRR4302542.4851411/94‑1 (MQ=60)
                                                 TCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATG                                            <  SRR4302542.3567213/94‑1 (MQ=60)
                                                 TCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATG                                            <  SRR4302542.5973696/94‑1 (MQ=60)
                                                 TCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATG                                            <  SRR4302542.4176845/94‑1 (MQ=60)
                                                  CGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGA                                           >  SRR4302542.357364/1‑94 (MQ=60)
                                                  CGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGA                                           <  SRR4302542.2419748/94‑1 (MQ=60)
                                                  CGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGA                                           >  SRR4302542.3140518/1‑94 (MQ=60)
                                                  CGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGA                                           >  SRR4302542.100612/1‑94 (MQ=60)
                                                  CGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGA                                           >  SRR4302542.1487728/1‑94 (MQ=60)
                                                    AGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAG                                         <  SRR4302542.791953/94‑1 (MQ=60)
                                                      ACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTG                                       <  SRR4302542.6885904/94‑1 (MQ=60)
                                                      ACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTG                                       <  SRR4302542.4393578/94‑1 (MQ=60)
                                                       CGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGT                                      >  SRR4302542.4247511/1‑94 (MQ=60)
                                                        GGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTA                                     <  SRR4302542.817312/94‑1 (MQ=60)
                                                        GGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGGGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTA                                     <  SRR4302542.5689183/94‑1 (MQ=60)
                                                         GAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAA                                    <  SRR4302542.5206382/94‑1 (MQ=60)
                                                         GAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAA                                    <  SRR4302542.903867/94‑1 (MQ=60)
                                                         GAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAA                                    >  SRR4302542.484535/1‑94 (MQ=60)
                                                          AAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAA                                   >  SRR4302542.460833/1‑94 (MQ=60)
                                                          AAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAA                                   >  SRR4302542.2994440/1‑94 (MQ=60)
                                                           AAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAAT                                  <  SRR4302542.5100488/94‑1 (MQ=60)
                                                           AAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAAT                                  >  SRR4302542.4580260/1‑94 (MQ=60)
                                                           AAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAAT                                  >  SRR4302542.3945638/1‑94 (MQ=60)
                                                            AGTGAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATA                                 <  SRR4302542.6745445/94‑1 (MQ=60)
                                                               GAATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAA                              <  SRR4302542.4942896/94‑1 (MQ=60)
                                                                AATGAGGGCCGGGGGGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAA                             <  SRR4302542.2221552/94‑1 (MQ=60)
                                                                AATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAA                             <  SRR4302542.7163149/94‑1 (MQ=60)
                                                                AATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAA                             <  SRR4302542.1799300/94‑1 (MQ=60)
                                                                AATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAA                             <  SRR4302542.6035673/94‑1 (MQ=60)
                                                                 ATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAA                            <  SRR4302542.3797448/94‑1 (MQ=60)
                                                                 ATGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAA                            >  SRR4302542.1865743/1‑94 (MQ=60)
                                                                 ATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAA                            <  SRR4302542.786399/94‑1 (MQ=60)
                                                                  TGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAG                           <  SRR4302542.1840140/94‑1 (MQ=60)
                                                                  TGAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAG                           >  SRR4302542.7194600/1‑94 (MQ=60)
                                                                   GAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGG                          <  SRR4302542.1838936/94‑1 (MQ=60)
                                                                   GAGGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGG                          >  SRR4302542.6646274/1‑94 (MQ=60)
                                                                     GGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAA                        <  SRR4302542.6949665/94‑1 (MQ=60)
                                                                     GGGCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAA                        <  SRR4302542.5325647/94‑1 (MQ=60)
                                                                     GGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAA                        <  SRR4302542.5319906/94‑1 (MQ=60)
                                                                       GCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACT                      <  SRR4302542.3907487/94‑1 (MQ=60)
                                                                       GCCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACT                      <  SRR4302542.5724533/94‑1 (MQ=60)
                                                                        CCGGGGAGCGTATTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTA                     <  SRR4302542.3534544/94‑1 (MQ=60)
                                                                        CCGGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTA                     <  SRR4302542.1594417/94‑1 (MQ=60)
                                                                        CCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTA                     <  SRR4302542.6519912/94‑1 (MQ=60)
                                                                         CGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTAT                    <  SRR4302542.4295250/94‑1 (MQ=60)
                                                                          GGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATT                   <  SRR4302542.4316134/94‑1 (MQ=60)
                                                                          GGGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATT                   >  SRR4302542.5286559/1‑94 (MQ=60)
                                                                           GGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTC                  <  SRR4302542.466903/94‑1 (MQ=60)
                                                                           GGGAGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTC                  >  SRR4302542.5398558/1‑94 (MQ=60)
                                                                           GGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTC                  >  SRR4302542.2018770/1‑94 (MQ=60)
                                                                              AGCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTG               >  SRR4302542.6876476/1‑94 (MQ=60)
                                                                              AGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTG               >  SRR4302542.1597377/1‑94 (MQ=60)
                                                                               GCGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGC              <  SRR4302542.5588234/94‑1 (MQ=60)
                                                                                CGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCA             >  SRR4302542.2842369/1‑94 (MQ=60)
                                                                                CGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCA             >  SRR4302542.6149970/1‑94 (MQ=60)
                                                                                CGTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCA             <  SRR4302542.7122540/94‑1 (MQ=60)
                                                                                CGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCA             <  SRR4302542.3193802/94‑1 (MQ=60)
                                                                                 GTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAG            <  SRR4302542.976616/94‑1 (MQ=60)
                                                                                 GTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAG            >  SRR4302542.2727436/1‑94 (MQ=60)
                                                                                 GTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAG            >  SRR4302542.5690937/1‑94 (MQ=60)
                                                                                 GTACTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAG            <  SRR4302542.6811297/94‑1 (MQ=60)
                                                                                 GTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAG            >  SRR4302542.2712917/1‑94 (MQ=60)
                                                                                 GTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAG            <  SRR4302542.2576205/94‑1 (MQ=60)
                                                                                   ACTCGCAGTACGTGACCCGGACGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGCAGTGTAAATAAAAAAGGAACTATTCTTGCAGTC          >  SRR4302542.2676670/1‑94 (MQ=60)
                                                                                    CTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCT         >  SRR4302542.1085880/1‑94 (MQ=60)
                                                                                    CTCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCT         <  SRR4302542.2148997/94‑1 (MQ=60)
                                                                                     TCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTC        <  SRR4302542.2757271/94‑1 (MQ=60)
                                                                                     TCGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTC        >  SRR4302542.6660378/1‑94 (MQ=60)
                                                                                      CGCAGTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCC       >  SRR4302542.6057350/1‑94 (MQ=60)
                                                                                          GTGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTCGT   >  SRR4302542.6682691/1‑94 (MQ=60)
                                                                                           TGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTCGTT  <  SRR4302542.513371/94‑1 (MQ=60)
                                                                                           TGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTCGTT  <  SRR4302542.5381350/94‑1 (MQ=60)
                                                                                           TGCGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTCGTT  <  SRR4302542.2104982/94‑1 (MQ=60)
                                                                                            |                                                                                            
ACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAGTCGTCGATGAGGCAGTGTGAAGGATGAAGTGTAAATAAAAAAGGAACTATTCTTGCAGTCTCCTCGTT  >  NC_000913/4456899‑4457083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑