Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,459,408 T→C Y276C (TAC→TGC)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,459,4080TC84.6% 694.9 / 117.2 306Y276C (TAC→TGC) nrdDanaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (25/22);  new base C (117/142);  total (142/164)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 306 total aligned reads displayed.

ATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGT  >  NC_000913/4459318‑4459499
                                                                                          |                                                                                           
aTCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCAc                                                                                           >  1:444005/1‑93 (MQ=255)
aTCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCAc                                                                                           >  1:596538/1‑93 (MQ=255)
 tCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACt                                                                                          <  1:733117/93‑1 (MQ=255)
   aGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTcc                                                                                        >  2:897258/1‑93 (MQ=255)
    ggCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCa                                                                                       >  2:3316022/1‑93 (MQ=255)
    ggCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCa                                                                                       <  2:3092992/93‑1 (MQ=255)
    ggCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCa                                                                                       >  2:6953922/1‑93 (MQ=255)
    ggCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCa                                                                                       >  1:284626/1‑93 (MQ=255)
     gcgcGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAg                                                                                      >  2:219391/1‑93 (MQ=255)
      cgcgATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGt                                                                                     <  2:6181320/93‑1 (MQ=255)
      cgcgATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGt                                                                                     <  1:5856163/93‑1 (MQ=255)
        cgATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGa                                                                                   >  1:4192342/1‑93 (MQ=255)
        cgATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGa                                                                                   >  1:6434767/1‑93 (MQ=255)
         gATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGAc                                                                                  <  2:2510546/93‑1 (MQ=255)
           ttCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTg                                                                                >  1:3723877/1‑93 (MQ=255)
              ccAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGaa                                                                             <  1:544169/93‑1 (MQ=255)
              ccAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGaa                                                                             <  2:2377098/93‑1 (MQ=255)
              ccAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGaa                                                                             <  1:4482308/93‑1 (MQ=255)
               cAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAg                                                                            <  2:4568260/93‑1 (MQ=255)
                aGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCCGACCGTTGGCGGTGTGTAGGGTGTTTACTTCGCACTCCAGTGACTGGAAgg                                                                           <  1:702871/93‑1 (MQ=255)
                 gCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGc                                                                          <  1:3835079/93‑1 (MQ=255)
                 gCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGc                                                                          <  2:764619/93‑1 (MQ=255)
                 gCTGGTGCCCAGGCAAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGc                                                                          <  1:6852846/93‑1 (MQ=255)
                  cTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCa                                                                         <  1:5756063/93‑1 (MQ=255)
                          cAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCa                                                                 <  1:1433871/93‑1 (MQ=255)
                          cAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCa                                                                 >  2:7422672/1‑93 (MQ=255)
                          cAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCa                                                                 >  1:5414832/1‑93 (MQ=255)
                          cAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCa                                                                 <  1:2266817/93‑1 (MQ=255)
                            ggCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCAct                                                               >  1:1710377/1‑93 (MQ=255)
                            ggCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCAct                                                               >  1:5367732/1‑93 (MQ=255)
                              ccAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCActct                                                             >  2:5110232/1‑93 (MQ=255)
                               cAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCtt                                                            <  1:749404/93‑1 (MQ=255)
                               cAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCtt                                                            >  2:3129309/1‑93 (MQ=255)
                               cAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCAACGTAGCACTCtt                                                            >  2:5665592/1‑93 (MQ=255)
                                aaaaCCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGGGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCAGCGTAGCACTCttt                                                           <  1:932392/93‑1 (MQ=255)
                                aaaaCCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCttt                                                           <  1:6613634/93‑1 (MQ=255)
                                aaaaCCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCttt                                                           >  1:7232371/1‑93 (MQ=255)
                                aaaaCCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCttt                                                           <  1:46257/93‑1 (MQ=255)
                                aaaaCCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCttt                                                           <  2:7107730/93‑1 (MQ=255)
                                aaaaCCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCttt                                                           <  2:2908311/93‑1 (MQ=255)
                                 aaaCCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTg                                                          <  2:2715974/93‑1 (MQ=255)
                                  aaCCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGa                                                         >  2:2603965/1‑93 (MQ=255)
                                    ccAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATg                                                       <  1:6083591/93‑1 (MQ=255)
                                    ccAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATg                                                       <  1:3447508/93‑1 (MQ=255)
                                     cAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATgg                                                      <  2:2875325/93‑1 (MQ=255)
                                     cAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATgg                                                      >  2:6009552/1‑93 (MQ=255)
                                     cAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATgg                                                      <  1:493897/93‑1 (MQ=255)
                                     cAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATgg                                                      <  1:781706/93‑1 (MQ=255)
                                      aaaGGTTACAAACGGCGTCTGACCATTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGt                                                     <  1:2633987/93‑1 (MQ=255)
                                       aaGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGtt                                                    >  1:4695146/1‑93 (MQ=255)
                                        aGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTc                                                   >  1:6256266/1‑93 (MQ=255)
                                         ggTTACAAACGGCGGCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCg                                                  >  2:7485757/1‑93 (MQ=255)
                                          gTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCga                                                 <  2:3988745/93‑1 (MQ=255)
                                          gTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCga                                                 <  2:5460952/93‑1 (MQ=255)
                                            tACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCgaga                                               <  1:4583513/93‑1 (MQ=255)
                                            tACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCgaga                                               >  1:6758252/1‑93 (MQ=255)
                                            tACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCgaga                                               <  1:2151679/93‑1 (MQ=255)
                                            tACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCgaga                                               <  1:1972074/93‑1 (MQ=255)
                                              cAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGt                                             <  1:3224141/93‑1 (MQ=255)
                                               aaaCGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGtt                                            <  2:5560719/93‑1 (MQ=255)
                                                 aCGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAg                                          <  2:5865962/93‑1 (MQ=255)
                                                   ggCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCg                                        >  2:22999/1‑93 (MQ=255)
                                                    gCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGt                                       >  1:3887286/1‑93 (MQ=255)
                                                     cGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTa                                      <  2:4971495/93‑1 (MQ=255)
                                                       tCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc                                    <  1:1542845/93‑1 (MQ=255)
                                                       tCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc                                    <  2:6701565/93‑1 (MQ=255)
                                                       tCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc                                    >  1:6323575/1‑93 (MQ=255)
                                                         tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCt                                  <  1:2209171/93‑1 (MQ=255)
                                                         tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCt                                  <  2:6389970/93‑1 (MQ=255)
                                                         tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCt                                  >  1:2454405/1‑93 (MQ=255)
                                                         tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCt                                  >  2:1578991/1‑93 (MQ=255)
                                                          gACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCtt                                 >  1:6963555/1‑93 (MQ=255)
                                                           aCCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTc                                >  2:5802755/1‑93 (MQ=255)
                                                            ccGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCg                               <  1:4826754/93‑1 (MQ=255)
                                                             cGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCgg                              >  2:3558270/1‑93 (MQ=255)
                                                             cGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCgg                              >  1:3159997/1‑93 (MQ=255)
                                                              gTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGc                             >  1:7073313/1‑93 (MQ=255)
                                                              gTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGc                             >  2:508460/1‑93 (MQ=255)
                                                                tGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGt                           <  2:379508/93‑1 (MQ=255)
                                                                  gCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTcc                         <  2:2147373/93‑1 (MQ=255)
                                                                   cGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCg                        <  2:3911269/93‑1 (MQ=255)
                                                                      tgtgCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGa                     >  2:381331/1‑93 (MQ=255)
                                                                       gtgCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGAt                    >  2:1781717/1‑93 (MQ=255)
                                                                         gCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGt                  >  2:4450460/1‑93 (MQ=255)
                                                                          cAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGtt                 >  2:5223685/1‑93 (MQ=255)
                                                                           aGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc                <  2:4137359/93‑1 (MQ=255)
                                                                           aGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc                <  2:661368/93‑1 (MQ=255)
                                                                           aGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc                >  2:7260746/1‑93 (MQ=255)
                                                                             ggTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCa              <  1:5138070/93‑1 (MQ=255)
                                                                                  ttACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACtctt         >  1:4084821/1‑93 (MQ=255)
                                                                                   tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACtcttc        <  1:1026706/93‑1 (MQ=255)
                                                                                   tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACtcttc        >  1:3518725/1‑93 (MQ=255)
                                                                                    aCTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACtcttct       <  1:6666595/93‑1 (MQ=255)
                                                                                    aCTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACtcttct       <  1:3985000/93‑1 (MQ=255)
                                                                                    aCTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACtcttct       <  1:2950189/93‑1 (MQ=255)
                                                                                    aCTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACtcttct       >  2:4689519/1‑93 (MQ=255)
                                                                                     cTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTg      <  2:6882678/93‑1 (MQ=255)
                                                                                      ttCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGc     >  2:1329177/1‑93 (MQ=255)
                                                                                      ttCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGc     >  2:4816735/1‑93 (MQ=255)
                                                                                         gTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGt  >  1:193355/1‑93 (MQ=255)
                                                                                          |                                                                                           
ATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGT  >  NC_000913/4459318‑4459499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTC  >  NC_000913/4459315‑4459500
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TGAATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGC                                                                                              <  SRR4302542.3542120/94‑1 (MQ=60)
  AATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTAC                                                                                            >  SRR4302542.1975466/1‑94 (MQ=60)
  AATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCAC                                                                                            >  SRR4302542.601680/1‑94 (MQ=60)
  AATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCAC                                                                                            >  SRR4302542.447851/1‑94 (MQ=60)
  AATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCAC                                                                                            <  SRR4302542.4008590/94‑1 (MQ=60)
   ATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACT                                                                                           <  SRR4302542.2442222/94‑1 (MQ=60)
    TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTC                                                                                          <  SRR4302542.739471/94‑1 (MQ=60)
     CAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCC                                                                                         <  SRR4302542.924361/94‑1 (MQ=60)
      AGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCA                                                                                        <  SRR4302542.7470711/94‑1 (MQ=60)
      AGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCA                                                                                        >  SRR4302542.287104/1‑94 (MQ=60)
        GCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGT                                                                                      <  SRR4302542.1919377/94‑1 (MQ=60)
         CGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTG                                                                                     <  SRR4302542.5912957/94‑1 (MQ=60)
         CGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTG                                                                                     <  SRR4302542.1450056/94‑1 (MQ=60)
         CGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTG                                                                                     <  SRR4302542.705915/94‑1 (MQ=60)
          GCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGA                                                                                    >  SRR4302542.6498164/1‑94 (MQ=60)
          GCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGA                                                                                    >  SRR4302542.4233676/1‑94 (MQ=60)
           CGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGAC                                                                                   <  SRR4302542.4734027/94‑1 (MQ=60)
           CGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGAC                                                                                   >  SRR4302542.6779036/1‑94 (MQ=60)
            GATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACT                                                                                  <  SRR4302542.6530327/94‑1 (MQ=60)
            GATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACT                                                                                  >  SRR4302542.228430/1‑94 (MQ=60)
            GATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACT                                                                                  >  SRR4302542.3731884/1‑94 (MQ=60)
            GATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACT                                                                                  >  SRR4302542.6193191/1‑94 (MQ=60)
            GATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACT                                                                                  >  SRR4302542.469324/1‑94 (MQ=60)
             ATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTG                                                                                 >  SRR4302542.2705673/1‑94 (MQ=60)
             ATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTG                                                                                 >  SRR4302542.2482215/1‑94 (MQ=60)
             ATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTG                                                                                 <  SRR4302542.699571/94‑1 (MQ=60)
             ATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTG                                                                                 >  SRR4302542.3760754/1‑94 (MQ=60)
              TTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGG                                                                                >  SRR4302542.5230908/1‑94 (MQ=60)
                 CCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAG                                                                             <  SRR4302542.5859674/94‑1 (MQ=60)
                 CCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAG                                                                             <  SRR4302542.548865/94‑1 (MQ=60)
                 CCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAG                                                                             <  SRR4302542.5475920/94‑1 (MQ=60)
                 CCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAG                                                                             <  SRR4302542.4526307/94‑1 (MQ=60)
                 CCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAG                                                                             <  SRR4302542.4818328/94‑1 (MQ=60)
                  CAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGG                                                                            <  SRR4302542.4228349/94‑1 (MQ=60)
                  CAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGG                                                                            >  SRR4302542.7205760/1‑94 (MQ=60)
                  CAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGG                                                                            <  SRR4302542.3921051/94‑1 (MQ=60)
                   AGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCCGACCGTTGGCGGTGTGTAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGC                                                                           <  SRR4302542.708957/94‑1 (MQ=60)
                    GCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCA                                                                          <  SRR4302542.3873008/94‑1 (MQ=60)
                    GCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCA                                                                          >  SRR4302542.7065056/1‑94 (MQ=60)
                    GCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCA                                                                          <  SRR4302542.7488858/94‑1 (MQ=60)
                    GCTGGTGCCCAGGCAAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCA                                                                          <  SRR4302542.6920202/94‑1 (MQ=60)
                     CTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCAT                                                                         <  SRR4302542.5811935/94‑1 (MQ=60)
                     CTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCAT                                                                         <  SRR4302542.323786/94‑1 (MQ=60)
                     CTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCAT                                                                         >  SRR4302542.1958398/1‑94 (MQ=60)
                      TGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATC                                                                        <  SRR4302542.6304622/94‑1 (MQ=60)
                       GGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCG                                                                       >  SRR4302542.787385/1‑94 (MQ=60)
                       GGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCG                                                                       <  SRR4302542.5765347/94‑1 (MQ=60)
                       GGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCG                                                                       <  SRR4302542.4030830/94‑1 (MQ=60)
                          GCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAG                                                                    <  SRR4302542.645636/94‑1 (MQ=60)
                          GCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAG                                                                    <  SRR4302542.4765487/94‑1 (MQ=60)
                            CCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCA                                                                  >  SRR4302542.5467417/1‑94 (MQ=60)
                             CAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCAC                                                                 <  SRR4302542.1446259/94‑1 (MQ=60)
                             CAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCAC                                                                 <  SRR4302542.2286325/94‑1 (MQ=60)
                              AGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACT                                                                >  SRR4302542.1725237/1‑94 (MQ=60)
                              AGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACT                                                                >  SRR4302542.5419898/1‑94 (MQ=60)
                                GCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCT                                                              <  SRR4302542.6733686/94‑1 (MQ=60)
                                  CAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTT                                                            >  SRR4302542.7303311/1‑94 (MQ=60)
                                  CAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTT                                                            <  SRR4302542.755887/94‑1 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGGGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCAGCGTAGCACTCTTTG                                                           <  SRR4302542.940414/94‑1 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           >  SRR4302542.4535337/1‑94 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           <  SRR4302542.46645/94‑1 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           <  SRR4302542.460556/94‑1 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           <  SRR4302542.352348/94‑1 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           <  SRR4302542.6678788/94‑1 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           <  SRR4302542.7353859/94‑1 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           >  SRR4302542.4125436/1‑94 (MQ=60)
                                   AAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGCGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTG                                                           >  SRR4302542.4947919/1‑94 (MQ=60)
                                    AAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGA                                                          >  SRR4302542.3946351/1‑94 (MQ=60)
                                     AACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGAT                                                         >  SRR4302542.5864792/1‑94 (MQ=60)
                                     AACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGAT                                                         <  SRR4302542.7100355/94‑1 (MQ=60)
                                      ACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATG                                                        >  SRR4302542.4642871/1‑94 (MQ=60)
                                      ACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATG                                                        <  SRR4302542.1426652/94‑1 (MQ=60)
                                       CCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGG                                                       <  SRR4302542.6142493/94‑1 (MQ=60)
                                       CCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGG                                                       <  SRR4302542.2532953/94‑1 (MQ=60)
                                       CCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGG                                                       <  SRR4302542.3479765/94‑1 (MQ=60)
                                       CCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGG                                                       >  SRR4302542.6131585/1‑94 (MQ=60)
                                       CCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGG                                                       <  SRR4302542.3839133/94‑1 (MQ=60)
                                        CAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGT                                                      <  SRR4302542.5424997/94‑1 (MQ=60)
                                        CAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGT                                                      <  SRR4302542.788456/94‑1 (MQ=60)
                                        CAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGT                                                      <  SRR4302542.498154/94‑1 (MQ=60)
                                        CAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGT                                                      <  SRR4302542.1726260/94‑1 (MQ=60)
                                         AAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTT                                                     >  SRR4302542.2477789/1‑94 (MQ=60)
                                         AAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTT                                                     >  SRR4302542.4740984/1‑94 (MQ=60)
                                         AAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTT                                                     >  SRR4302542.5598199/1‑94 (MQ=60)
                                         AAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTT                                                     >  SRR4302542.4895269/1‑94 (MQ=60)
                                         AAAGGTTACAAACGGCGTCTGACCATTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTT                                                     <  SRR4302542.2659429/94‑1 (MQ=60)
                                          AAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTC                                                    >  SRR4302542.6317909/1‑94 (MQ=60)
                                          AAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTC                                                    >  SRR4302542.5953114/1‑94 (MQ=60)
                                           AGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCG                                                   >  SRR4302542.219986/1‑94 (MQ=60)
                                             GTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAG                                                 >  SRR4302542.4521398/1‑94 (MQ=60)
                                             GTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAG                                                 >  SRR4302542.5492982/1‑94 (MQ=60)
                                              TTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGA                                                >  SRR4302542.6824727/1‑94 (MQ=60)
                                               TACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAG                                               <  SRR4302542.4628355/94‑1 (MQ=60)
                                               TACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAG                                               <  SRR4302542.1989113/94‑1 (MQ=60)
                                               TACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAG                                               >  SRR4302542.7587763/1‑94 (MQ=60)
                                               TACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAG                                               <  SRR4302542.3161987/94‑1 (MQ=60)
                                               TACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAG                                               <  SRR4302542.2170226/94‑1 (MQ=60)
                                                ACAAACGGCGTCTGACCGTAGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGT                                              >  SRR4302542.7141076/1‑94 (MQ=60)
                                                 CAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTT                                             <  SRR4302542.3254651/94‑1 (MQ=60)
                                                  AAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTA                                            >  SRR4302542.4066761/1‑94 (MQ=60)
                                                   AACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAG                                           <  SRR4302542.5423222/94‑1 (MQ=60)
                                                   AACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAG                                           <  SRR4302542.3745125/94‑1 (MQ=60)
                                                   AACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAG                                           <  SRR4302542.3620391/94‑1 (MQ=60)
                                                   AACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAG                                           <  SRR4302542.2889399/94‑1 (MQ=60)
                                                   AACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAG                                           <  SRR4302542.5999265/94‑1 (MQ=60)
                                                    ACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGC                                          <  SRR4302542.3270865/94‑1 (MQ=60)
                                                     CGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCG                                         <  SRR4302542.5060249/94‑1 (MQ=60)
                                                      GGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGT                                        >  SRR4302542.3925666/1‑94 (MQ=60)
                                                        CGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAG                                      <  SRR4302542.4992340/94‑1 (MQ=60)
                                                        CGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAG                                      <  SRR4302542.694851/94‑1 (MQ=60)
                                                         GTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGC                                     >  SRR4302542.225534/1‑94 (MQ=60)
                                                         GTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGC                                     >  SRR4302542.7475601/1‑94 (MQ=60)
                                                         GTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGC                                     >  SRR4302542.6385893/1‑94 (MQ=60)
                                                          TCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCC                                    <  SRR4302542.1556162/94‑1 (MQ=60)
                                                          TCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCC                                    <  SRR4302542.1480284/94‑1 (MQ=60)
                                                           CTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCT                                   >  SRR4302542.5301637/1‑94 (MQ=60)
                                                           CTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCT                                   >  SRR4302542.2478271/1‑94 (MQ=60)
                                                            TGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTT                                  >  SRR4302542.21644/1‑94 (MQ=60)
                                                            TGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTT                                  <  SRR4302542.2228185/94‑1 (MQ=60)
                                                            TGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTT                                  >  SRR4302542.7031970/1‑94 (MQ=60)
                                                            TGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTT                                  >  SRR4302542.1276436/1‑94 (MQ=60)
                                                            TGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTT                                  >  SRR4302542.2985290/1‑94 (MQ=60)
                                                             GACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTC                                 >  SRR4302542.6731666/1‑94 (MQ=60)
                                                             GACCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTC                                 >  SRR4302542.4497689/1‑94 (MQ=60)
                                                              ACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCG                                <  SRR4302542.1714340/94‑1 (MQ=60)
                                                               CCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGG                               >  SRR4302542.3189993/1‑94 (MQ=60)
                                                               CCGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGG                               <  SRR4302542.4873790/94‑1 (MQ=60)
                                                                CGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGC                              >  SRR4302542.7142729/1‑94 (MQ=60)
                                                                CGTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGC                              >  SRR4302542.4416806/1‑94 (MQ=60)
                                                                 GTTGGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCG                             <  SRR4302542.6875633/94‑1 (MQ=60)
                                                                    GGCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCC                          >  SRR4302542.3236515/1‑94 (MQ=60)
                                                                     GCGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCG                         <  SRR4302542.2039806/94‑1 (MQ=60)
                                                                      CGGTGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGG                        <  SRR4302542.4002355/94‑1 (MQ=60)
                                                                       GGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGG                       <  SRR4302542.6055146/94‑1 (MQ=60)
                                                                         TGTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGAT                     <  SRR4302542.2921201/94‑1 (MQ=60)
                                                                          GTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATG                    >  SRR4302542.3496264/1‑94 (MQ=60)
                                                                          GTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATG                    <  SRR4302542.3297548/94‑1 (MQ=60)
                                                                          GTGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATG                    >  SRR4302542.4665203/1‑94 (MQ=60)
                                                                           TGCAGGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGT                   >  SRR4302542.1573056/1‑94 (MQ=60)
                                                                             CAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTC                 >  SRR4302542.3909660/1‑94 (MQ=60)
                                                                             CAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTC                 >  SRR4302542.7316253/1‑94 (MQ=60)
                                                                               GGGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCA               <  SRR4302542.809960/94‑1 (MQ=60)
                                                                                GGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCAC              <  SRR4302542.672335/94‑1 (MQ=60)
                                                                                GGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCAC              <  SRR4302542.5188010/94‑1 (MQ=60)
                                                                                GGTGTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCAC              >  SRR4302542.6535002/1‑94 (MQ=60)
                                                                                 GTGTNNANTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACT             <  SRR4302542.6903289/94‑1 (MQ=60)
                                                                                   GTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCT           <  SRR4302542.4504314/94‑1 (MQ=60)
                                                                                   GTTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCT           >  SRR4302542.6422796/1‑94 (MQ=60)
                                                                                    TTTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTT          >  SRR4302542.4125073/1‑94 (MQ=60)
                                                                                     TTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTC         >  SRR4302542.3551615/1‑94 (MQ=60)
                                                                                     TTACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTC         <  SRR4302542.7293078/94‑1 (MQ=60)
                                                                                      TACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCT        <  SRR4302542.1035555/94‑1 (MQ=60)
                                                                                      TACTTCGCCCTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCT        <  SRR4302542.3803978/94‑1 (MQ=60)
                                                                                       ACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTG       <  SRR4302542.2978429/94‑1 (MQ=60)
                                                                                       ACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTG       <  SRR4302542.4024314/94‑1 (MQ=60)
                                                                                       ACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTG       >  SRR4302542.5693074/1‑94 (MQ=60)
                                                                                       ACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTG       >  SRR4302542.4463974/1‑94 (MQ=60)
                                                                                       ACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTG       >  SRR4302542.2740161/1‑94 (MQ=60)
                                                                                       ACTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTG       <  SRR4302542.6732276/94‑1 (MQ=60)
                                                                                        CTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGC      >  SRR4302542.6368612/1‑94 (MQ=60)
                                                                                        CTTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGC      >  SRR4302542.2175602/1‑94 (MQ=60)
                                                                                         TTCGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCG     <  SRR4302542.6323740/94‑1 (MQ=60)
                                                                                           CGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGT   >  SRR4302542.194992/1‑94 (MQ=60)
                                                                                           CGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGT   <  SRR4302542.1873792/94‑1 (MQ=60)
                                                                                           CGCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGT   >  SRR4302542.2041124/1‑94 (MQ=60)
                                                                                            GCACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTC  <  SRR4302542.170185/94‑1 (MQ=60)
                                                                                             |                                                                                            
TGAATCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTC  >  NC_000913/4459315‑4459500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑