Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,555,245	T→C	Y205C (TAC→TGC)	yjiE ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,555,245	0	T	C	87.0%	757.5 / 90.4	301	Y205C (TAC→TGC)	yjiE	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base T (23/16); new base C (133/129); total (156/145)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01

Only 100 of 301 total aligned reads displayed.

TTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA  >  NC_000913/4555155‑4555336

tttAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAg                                                                                           >  1:4459551/1‑93 (MQ=255)

  tAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGa                                                                                         <  2:365170/93‑1 (MQ=255)

  tAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGa                                                                                         <  1:6941591/93‑1 (MQ=255)

  tAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGa                                                                                         >  2:744245/1‑93 (MQ=255)

    aaaGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGt                                                                                       >  1:4343284/1‑93 (MQ=255)

    aaaGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGt                                                                                       <  1:3110676/93‑1 (MQ=255)

    aaaGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGt                                                                                       >  2:2245579/1‑93 (MQ=255)

      aGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTg                                                                                     <  2:4667629/93‑1 (MQ=255)

       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTGACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGc                                                                                    <  2:7256472/93‑1 (MQ=255)

       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGCTGc                                                                                    >  2:5810785/1‑93 (MQ=255)

       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGc                                                                                    >  2:4816460/1‑93 (MQ=255)

         tcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCgg                                                                                  >  1:5218682/1‑93 (MQ=255)

         tcgctcATCGAAGAGACAAAAAAGGAGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCgg                                                                                  >  2:1464130/1‑93 (MQ=255)

             tcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGt                                                                              >  1:437686/1‑93 (MQ=255)

             tcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGt                                                                              >  2:1931747/1‑93 (MQ=255)

              cATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTa                                                                             >  2:2120202/1‑93 (MQ=255)

               aTCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTaa                                                                            <  1:7106949/93‑1 (MQ=255)

               aTCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTaa                                                                            >  1:5460531/1‑93 (MQ=255)

                tCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAt                                                                           >  1:7401733/1‑93 (MQ=255)

                 cGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAtt                                                                          <  1:1296034/93‑1 (MQ=255)

                  gAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTc                                                                         >  2:5382511/1‑93 (MQ=255)

                   aaGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGCAATTCa                                                                        <  2:6988238/93‑1 (MQ=255)

                      agaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTa                                                                     >  1:1007982/1‑93 (MQ=255)

                      agaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTa                                                                     >  1:6102986/1‑93 (MQ=255)

                       gaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTaa                                                                    <  1:4747940/93‑1 (MQ=255)

                       gaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTaa                                                                    <  1:1390385/93‑1 (MQ=255)

                       gaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCACGCAGGAGTTGCGGCTGTAATTCAGTaa                                                                    <  2:6416312/93‑1 (MQ=255)

                        aCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAc                                                                   >  1:1158234/1‑93 (MQ=255)

                         cAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACg                                                                  <  2:5874922/93‑1 (MQ=255)

                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                                 >  2:5031233/1‑93 (MQ=255)

                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                                 >  1:5076658/1‑93 (MQ=255)

                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                                 <  1:3029312/93‑1 (MQ=255)

                           aaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGa                                                                >  2:4091835/1‑93 (MQ=255)

                           aaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGa                                                                <  2:5747770/93‑1 (MQ=255)

                            aaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaa                                                               >  2:3794502/1‑93 (MQ=255)

                            aaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaa                                                               <  2:3493737/93‑1 (MQ=255)

                             aaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGaaa                                                              <  2:6883746/93‑1 (MQ=255)

                             aaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaaa                                                              <  1:3998783/93‑1 (MQ=255)

                             aaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaaa                                                              >  1:1691749/1‑93 (MQ=255)

                               aGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgt                                                            >  2:5644430/1‑93 (MQ=255)

                               aGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgt                                                            >  1:1768307/1‑93 (MQ=255)

                                 gTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgtgt                                                          >  1:4550120/1‑93 (MQ=255)

                                 gTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgtgt                                                          <  1:6448004/93‑1 (MQ=255)

                                 gTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgtgt                                                          >  2:2386303/1‑93 (MQ=255)

                                  tGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGtgtg                                                         >  2:3413861/1‑93 (MQ=255)

                                   gCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTgg                                                        <  2:5781467/93‑1 (MQ=255)

                                    cTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGc                                                       <  1:4944528/93‑1 (MQ=255)

                                    cTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGc                                                       <  1:1462100/93‑1 (MQ=255)

                                     tGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCt                                                      <  1:6003049/93‑1 (MQ=255)

                                        aaCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcg                                                   >  1:3254441/1‑93 (MQ=255)

                                        aaCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcg                                                   >  2:7515725/1‑93 (MQ=255)

                                         aCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcgc                                                  >  1:985909/1‑93 (MQ=255)

                                         aCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcgc                                                  >  2:6732500/1‑93 (MQ=255)

                                           ttAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCACCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGa                                                <  2:7098096/93‑1 (MQ=255)

                                              aCTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGt                                             >  1:3584319/1‑93 (MQ=255)

                                              aCTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGt                                             >  1:2645932/1‑93 (MQ=255)

                                                tCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTa                                           >  1:386444/1‑93 (MQ=255)

                                                 cACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaa                                          <  2:1801756/93‑1 (MQ=255)

                                                 cACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaa                                          >  1:813149/1‑93 (MQ=255)

                                                  aCTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaa                                         >  2:2459825/1‑93 (MQ=255)

                                                  aCTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaa                                         >  2:2767764/1‑93 (MQ=255)

                                                    tgtgGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaaaa                                       >  1:2199522/1‑93 (MQ=255)

                                                             tCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtctc                              <  1:4137833/93‑1 (MQ=255)

                                                              cAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTcc                             <  1:6335298/93‑1 (MQ=255)

                                                              cAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTcc                             <  2:4440112/93‑1 (MQ=255)

                                                               aGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCg                            >  2:2586653/1‑93 (MQ=255)

                                                               aGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCg                            >  2:5197522/1‑93 (MQ=255)

                                                               aGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCg                            >  2:7184730/1‑93 (MQ=255)

                                                                gggTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:37020/93‑1 (MQ=255)

                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:1051716/93‑1 (MQ=255)

                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:2457641/93‑1 (MQ=255)

                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  2:2975333/93‑1 (MQ=255)

                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                           <  1:2853375/93‑1 (MQ=255)

                                                                   tGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGtt                        <  1:277690/93‑1 (MQ=255)

                                                                    gCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTGAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTc                       >  2:6275211/1‑93 (MQ=255)

                                                                    gCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTc                       >  1:483688/1‑93 (MQ=255)

                                                                      gATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGt                     >  2:7286825/1‑93 (MQ=255)

                                                                      gATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGt                     >  1:1860251/1‑93 (MQ=255)

                                                                       aTTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTc                    <  2:3692476/93‑1 (MQ=255)

                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  >  2:607194/1‑93 (MQ=255)

                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  <  1:2321252/93‑1 (MQ=255)

                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  <  2:1182792/93‑1 (MQ=255)

                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGAGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                  <  2:1288555/93‑1 (MQ=255)

                                                                          aatcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACt                 <  2:6535303/93‑1 (MQ=255)

                                                                          aatcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGACTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACt                 <  2:7381284/93‑1 (MQ=255)

                                                                            tcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTgg               <  1:670597/93‑1 (MQ=255)

                                                                            tcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTgg               <  2:3136509/93‑1 (MQ=255)

                                                                            tcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTgg               <  1:4670000/93‑1 (MQ=255)

                                                                             caatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGgc              <  2:428926/93‑1 (MQ=255)

                                                                               atcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGgcgc            >  2:5339461/1‑93 (MQ=255)

                                                                                tcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCa           <  1:5392613/93‑1 (MQ=255)

                                                                                  gCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGa         <  2:1621690/93‑1 (MQ=255)

                                                                                   ccccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGAc        <  2:1758205/93‑1 (MQ=255)

                                                                                    cccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACa       >  2:492422/1‑93 (MQ=255)

                                                                                     ccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAg      >  2:5723987/1‑93 (MQ=255)

                                                                                     ccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAg      >  2:3282110/1‑93 (MQ=255)

                                                                                     ccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAg      <  1:2073004/93‑1 (MQ=255)

                                                                                      cATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAgg     >  1:5546677/1‑93 (MQ=255)

                                                                                       aTGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAggg    <  2:6228866/93‑1 (MQ=255)

                                                                                         gTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGaa  <  2:3994415/93‑1 (MQ=255)

TTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA  >  NC_000913/4555155‑4555336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  >  NC_000913/4555152‑4555338

TGCTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGC                                                                                               >  SRR4302542.6336758/1‑94 (MQ=60)

  CTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAG                                                                                             >  SRR4302542.4519706/1‑94 (MQ=60)

  CTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAG                                                                                             >  SRR4302542.4503297/1‑94 (MQ=60)

   TTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGG                                                                                            >  SRR4302542.732155/1‑94 (MQ=60)

    TTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCGCTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGA                                                                                           <  SRR4302542.1042419/94‑1 (MQ=60)

     TAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAG                                                                                          <  SRR4302542.3049904/94‑1 (MQ=60)

     TAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAG                                                                                          <  SRR4302542.7009794/94‑1 (MQ=60)

      AAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGT                                                                                         >  SRR4302542.4385952/1‑94 (MQ=60)

      AAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGT                                                                                         >  SRR4302542.5048697/1‑94 (MQ=60)

      AAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGT                                                                                         >  SRR4302542.7137332/1‑94 (MQ=60)

       AAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTT                                                                                        <  SRR4302542.4784368/94‑1 (MQ=60)

       AAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTT                                                                                        <  SRR4302542.3140235/94‑1 (MQ=60)

       AAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTT                                                                                        <  SRR4302542.2163982/94‑1 (MQ=60)

        AAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTG                                                                                       <  SRR4302542.1048964/94‑1 (MQ=60)

         AGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGC                                                                                      <  SRR4302542.818524/94‑1 (MQ=60)

         AGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGC                                                                                      <  SRR4302542.2138677/94‑1 (MQ=60)

          GCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCG                                                                                     <  SRR4302542.4655659/94‑1 (MQ=60)

          GCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCG                                                                                     >  SRR4302542.642413/1‑94 (MQ=60)

          GCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCG                                                                                     >  SRR4302542.1624321/1‑94 (MQ=60)

          GCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCG                                                                                     <  SRR4302542.2352786/94‑1 (MQ=60)

          GCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCG                                                                                     >  SRR4302542.883868/1‑94 (MQ=60)

           CTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGG                                                                                    >  SRR4302542.5269433/1‑94 (MQ=60)

           CTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGG                                                                                    >  SRR4302542.2499935/1‑94 (MQ=60)

            TCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGC                                                                                   >  SRR4302542.4987587/1‑94 (MQ=60)

             CGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCT                                                                                  >  SRR4302542.4325417/1‑94 (MQ=60)

               CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGT                                                                                >  SRR4302542.441476/1‑94 (MQ=60)

               CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                >  SRR4302542.5205134/1‑94 (MQ=60)

               CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                >  SRR4302542.1728970/1‑94 (MQ=60)

               CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                <  SRR4302542.4310679/94‑1 (MQ=60)

               CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGGGCGATTAATCAATCGCCCCATGCAGGCGTTGCGGCTGT                                                                                >  SRR4302542.7122543/1‑94 (MQ=60)

                 CATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAA                                                                              >  SRR4302542.5513583/1‑94 (MQ=60)

                  ATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAT                                                                             >  SRR4302542.3936056/1‑94 (MQ=60)

                  ATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAT                                                                             <  SRR4302542.5491342/94‑1 (MQ=60)

                  ATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAT                                                                             <  SRR4302542.7176708/94‑1 (MQ=60)

                  ATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAT                                                                             >  SRR4302542.7474165/1‑94 (MQ=60)

                  ATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAT                                                                             >  SRR4302542.1742804/1‑94 (MQ=60)

                  ATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAT                                                                             >  SRR4302542.4469251/1‑94 (MQ=60)

                    CGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTC                                                                           <  SRR4302542.5851252/94‑1 (MQ=60)

                    CGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTC                                                                           <  SRR4302542.858299/94‑1 (MQ=60)

                    CGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTC                                                                           <  SRR4302542.1307195/94‑1 (MQ=60)

                     GAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCA                                                                          >  SRR4302542.2694546/1‑94 (MQ=60)

                      AAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAG                                                                         >  SRR4302542.5564386/1‑94 (MQ=60)

                       AGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGT                                                                        <  SRR4302542.6148653/94‑1 (MQ=60)

                        GAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTA                                                                       >  SRR4302542.1016664/1‑94 (MQ=60)

                        GAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTA                                                                       >  SRR4302542.6162045/1‑94 (MQ=60)

                          GACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAC                                                                     >  SRR4302542.4927102/1‑94 (MQ=60)

                          GACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAC                                                                     <  SRR4302542.65661/94‑1 (MQ=60)

                          GACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAC                                                                     >  SRR4302542.1168163/1‑94 (MQ=60)

                          GACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAC                                                                     <  SRR4302542.4794213/94‑1 (MQ=60)

                          GACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAC                                                                     <  SRR4302542.1269372/94‑1 (MQ=60)

                          GACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAC                                                                     <  SRR4302542.1402393/94‑1 (MQ=60)

                            CAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGG                                                                   >  SRR4302542.5125990/1‑94 (MQ=60)

                            CAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGG                                                                   <  SRR4302542.2376074/94‑1 (MQ=60)

                            CAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGG                                                                   >  SRR4302542.3821002/1‑94 (MQ=60)

                             AAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGA                                                                  <  SRR4302542.3058224/94‑1 (MQ=60)

                             AAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGA                                                                  >  SRR4302542.5116535/1‑94 (MQ=60)

                             AAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGA                                                                  >  SRR4302542.622002/1‑94 (MQ=60)

                               AAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAA                                                                >  SRR4302542.1706396/1‑94 (MQ=60)

                                AAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAG                                                               <  SRR4302542.4038223/94‑1 (MQ=60)

                                 AAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGT                                                              >  SRR4302542.1783639/1‑94 (MQ=60)

                                   GGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGT                                                            >  SRR4302542.4594652/1‑94 (MQ=60)

                                   GGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGT                                                            <  SRR4302542.4795472/94‑1 (MQ=60)

                                    GTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTG                                                           <  SRR4302542.6511506/94‑1 (MQ=60)

                                    GTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTG                                                           <  SRR4302542.1229215/94‑1 (MQ=60)

                                       CTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCT                                                        >  SRR4302542.365170/1‑94 (MQ=60)

                                       CTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCT                                                        <  SRR4302542.4992571/94‑1 (MQ=60)

                                       CTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCT                                                        <  SRR4302542.1474724/94‑1 (MQ=60)

                                        TGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTG                                                       <  SRR4302542.6061187/94‑1 (MQ=60)

                                        TGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTG                                                       <  SRR4302542.3108597/94‑1 (MQ=60)

                                        TGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTG                                                       <  SRR4302542.261025/94‑1 (MQ=60)

                                          AAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCG                                                     >  SRR4302542.3545213/1‑94 (MQ=60)

                                          AAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCG                                                     >  SRR4302542.3285175/1‑94 (MQ=60)

                                           AACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGC                                                    <  SRR4302542.7209003/94‑1 (MQ=60)

                                           AACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGC                                                    >  SRR4302542.994405/1‑94 (MQ=60)

                                           AACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGC                                                    <  SRR4302542.6326594/94‑1 (MQ=60)

                                           AACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGC                                                    >  SRR4302542.1843466/1‑94 (MQ=60)

                                           AACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGC                                                    >  SRR4302542.2813251/1‑94 (MQ=60)

                                           AACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGC                                                    >  SRR4302542.6431930/1‑94 (MQ=60)

                                            ACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCG                                                   >  SRR4302542.1671810/1‑94 (MQ=60)

                                            ACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCG                                                   <  SRR4302542.1312576/94‑1 (MQ=60)

                                            ACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCG                                                   <  SRR4302542.6096702/94‑1 (MQ=60)

                                             CTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGGAACGGAAAGGGTGGCTGCGCGA                                                  >  SRR4302542.6672176/1‑94 (MQ=60)

                                              TTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAG                                                 <  SRR4302542.4678068/94‑1 (MQ=60)

                                               TAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCACATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGG                                                <  SRR4302542.1351646/94‑1 (MQ=60)

                                                AACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGT                                               >  SRR4302542.2671474/1‑94 (MQ=60)

                                                AACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGT                                               >  SRR4302542.3617750/1‑94 (MQ=60)

                                                 ACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTT                                              <  SRR4302542.3146679/94‑1 (MQ=60)

                                                 ACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTT                                              <  SRR4302542.1795861/94‑1 (MQ=60)

                                                 ACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTT                                              <  SRR4302542.6856325/94‑1 (MQ=60)

                                                 ACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGT                                              >  SRR4302542.4393718/1‑94 (MQ=60)

                                                  CTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTA                                             >  SRR4302542.389798/1‑94 (MQ=60)

                                                  CTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTA                                             <  SRR4302542.3773263/94‑1 (MQ=60)

                                                   TCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAA                                            >  SRR4302542.820186/1‑94 (MQ=60)

                                                      CTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAA                                         >  SRR4302542.2218466/1‑94 (MQ=60)

                                                      CTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAA                                         <  SRR4302542.3926292/94‑1 (MQ=60)

                                                       TGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAG                                        <  SRR4302542.5400806/94‑1 (MQ=60)

                                                        GTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGT                                       <  SRR4302542.1166399/94‑1 (MQ=60)

                                                        GTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGT                                       <  SRR4302542.2253780/94‑1 (MQ=60)

                                                          GGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGC                                     <  SRR4302542.1100942/94‑1 (MQ=60)

                                                            CGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTT                                   >  SRR4302542.268539/1‑94 (MQ=60)

                                                               GTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTC                                >  SRR4302542.3473578/1‑94 (MQ=60)

                                                               GTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTC                                >  SRR4302542.2923260/1‑94 (MQ=60)

                                                                TCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCC                               >  SRR4302542.5784035/1‑94 (MQ=60)

                                                                TCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCC                               <  SRR4302542.4178604/94‑1 (MQ=60)

                                                                 CAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCG                              >  SRR4302542.4763714/1‑94 (MQ=60)

                                                                 CAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCG                              <  SRR4302542.6397717/94‑1 (MQ=60)

                                                                  AGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGT                             >  SRR4302542.188935/1‑94 (MQ=60)

                                                                  AGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGT                             >  SRR4302542.551671/1‑94 (MQ=60)

                                                                   GGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTG                            <  SRR4302542.37349/94‑1 (MQ=60)

                                                                   GGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTG                            <  SRR4302542.1060762/94‑1 (MQ=60)

                                                                   GGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTG                            <  SRR4302542.2481518/94‑1 (MQ=60)

                                                                   GGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTG                            <  SRR4302542.2880794/94‑1 (MQ=60)

                                                                      TGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         <  SRR4302542.280065/94‑1 (MQ=60)

                                                                      TGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         >  SRR4302542.487878/1‑94 (MQ=60)

                                                                      TGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         >  SRR4302542.2818047/1‑94 (MQ=60)

                                                                       GCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCG                        <  SRR4302542.6962515/94‑1 (MQ=60)

                                                                        CGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGT                       >  SRR4302542.441086/1‑94 (MQ=60)

                                                                        CGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGT                       >  SRR4302542.1965110/1‑94 (MQ=60)

                                                                        CGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGT                       >  SRR4302542.3131138/1‑94 (MQ=60)

                                                                        CGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGT                       >  SRR4302542.1876350/1‑94 (MQ=60)

                                                                            TAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACT                   <  SRR4302542.4858754/94‑1 (MQ=60)

                                                                            TAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACT                   <  SRR4302542.2341151/94‑1 (MQ=60)

                                                                             AATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTG                  <  SRR4302542.5261183/94‑1 (MQ=60)

                                                                               TCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGC                <  SRR4302542.676383/94‑1 (MQ=60)

                                                                               TCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGC                <  SRR4302542.4715642/94‑1 (MQ=60)

                                                                               TCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGC                <  SRR4302542.3154269/94‑1 (MQ=60)

                                                                                   TCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAG            <  SRR4302542.5444993/94‑1 (MQ=60)

                                                                                    CGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGA           >  SRR4302542.4553614/1‑94 (MQ=60)

                                                                                    CGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGAGCTTCTCCGTGTTCGTCACTGGCGCAGA           >  SRR4302542.4121154/1‑94 (MQ=60)

                                                                                     GCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGAC          >  SRR4302542.5454908/1‑94 (MQ=60)

                                                                                     GCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGAC          >  SRR4302542.4455326/1‑94 (MQ=60)

                                                                                       CCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAG        >  SRR4302542.1420789/1‑94 (MQ=60)

                                                                                        CCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGG       >  SRR4302542.7563405/1‑94 (MQ=60)

                                                                                        CCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGG       >  SRR4302542.2244712/1‑94 (MQ=60)

                                                                                        CCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGG       <  SRR4302542.5429441/94‑1 (MQ=60)

                                                                                        CCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGG       >  SRR4302542.5600577/1‑94 (MQ=60)

                                                                                        CCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGG       <  SRR4302542.2090919/94‑1 (MQ=60)

                                                                                         CATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGG      <  SRR4302542.1429790/94‑1 (MQ=60)

                                                                                         CATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGG      <  SRR4302542.4142000/94‑1 (MQ=60)

                                                                                           TGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA    <  SRR4302542.6207190/94‑1 (MQ=60)

                                                                                           TGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA    >  SRR4302542.3775797/1‑94 (MQ=60)

                                                                                           TGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA    <  SRR4302542.1543185/94‑1 (MQ=60)

                                                                                           TGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAA    <  SRR4302542.4315000/94‑1 (MQ=60)

                                                                                            GCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAAC   <  SRR4302542.628176/94‑1 (MQ=60)

                                                                                            GCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAAC   <  SRR4302542.6518311/94‑1 (MQ=60)

                                                                                            GCAGGAGTTGCGGCTGAAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAAC   <  SRR4302542.5103390/94‑1 (MQ=60)

                                                                                             CAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  >  SRR4302542.2274847/1‑94 (MQ=60)

TGCTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  >  NC_000913/4555152‑4555338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`