Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,093	T→C	V173A (GTC→GCC)	creB →	CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,093	0	T	C	84.6%	596.8 / 95.8	267	V173A (GTC→GCC)	creB	CreB
Reads supporting (aligned to +/- strand): ref base T (21/20); new base C (111/115); total (132/135)
Fisher's exact test for biased strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 273 total aligned reads displayed.

CGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTG  >  NC_000913/4634003‑4634185

 ggcgCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTg                                                                                           >  2:5590366/1‑93 (MQ=255)

 ggcgCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTg                                                                                           >  1:2979373/1‑93 (MQ=255)

     cAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTcc                                                                                       <  1:5774270/93‑1 (MQ=255)

       gATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCg                                                                                     >  1:5366117/1‑93 (MQ=255)

         tCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGcc                                                                                   <  1:2147541/93‑1 (MQ=255)

         tCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCACCTGGTCCCGcc                                                                                   >  1:172072/1‑93 (MQ=255)

          cggCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCa                                                                                  <  1:4610259/91‑1 (MQ=255)

           ggCTGTTTTGACACGCCATTAGCGCTGCCTCGGTATGAGTTTTTATTGTTAAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAg                                                                                 <  1:6416972/92‑1 (MQ=255)

            gCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGc                                                                                >  1:5715789/1‑93 (MQ=255)

            gCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGc                                                                                >  2:4120190/1‑93 (MQ=255)

                   tGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGAt                                                                         <  1:7425585/93‑1 (MQ=255)

                    gACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATg                                                                        >  2:6712750/1‑93 (MQ=255)

                    gACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAAACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATg                                                                        >  2:1883585/1‑93 (MQ=255)

                     acacGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATgg                                                                       >  1:941879/1‑93 (MQ=255)

                      cacGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGa                                                                      <  1:5952110/93‑1 (MQ=255)

                      cacGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGa                                                                      <  2:3585836/93‑1 (MQ=255)

                       acGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGAt                                                                     <  2:6301664/93‑1 (MQ=255)

                       acGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGAt                                                                     <  1:2061437/93‑1 (MQ=255)

                         gCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAg                                                                   >  2:2132499/1‑93 (MQ=255)

                          ccATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGc                                                                  <  2:6552180/93‑1 (MQ=255)

                           cATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCg                                                                 <  2:6857125/93‑1 (MQ=255)

                            aTTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGt                                                                >  2:4743156/1‑93 (MQ=255)

                            aTTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGt                                                                >  2:5976230/1‑93 (MQ=255)

                            aTTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGt                                                                >  1:2431352/1‑93 (MQ=255)

                             ttAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTa                                                               <  1:6763116/93‑1 (MQ=255)

                             ttAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTa                                                               <  2:461155/93‑1 (MQ=255)

                             ttAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTa                                                               >  1:1283933/1‑93 (MQ=255)

                             ttAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTAACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTa                                                               >  1:7302225/1‑93 (MQ=255)

                              tggcgcTGCCTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTAt                                                              <  1:3811925/91‑1 (MQ=255)

                               aGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATg                                                             <  2:5724328/93‑1 (MQ=255)

                                gcgcTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATgg                                                            >  1:1873255/1‑93 (MQ=255)

                                gcgcTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATgg                                                            >  1:3380143/1‑93 (MQ=255)

                                  gcTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGa                                                          >  2:3941273/1‑93 (MQ=255)

                                     gACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGa                                                       >  1:142407/1‑93 (MQ=255)

                                     gACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGa                                                       <  1:2925440/93‑1 (MQ=255)

                                     gACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGa                                                       <  1:6184551/93‑1 (MQ=255)

                                     gACTCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGa                                                       >  2:4844826/1‑93 (MQ=255)

                                      aCTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAt                                                      >  2:2510528/1‑93 (MQ=255)

                                      aCTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAt                                                      <  2:623056/93‑1 (MQ=255)

                                        tgggTATGGTTTTTTATTTTTGAAGACGTTCCTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATgc                                                    <  1:2768409/91‑1 (MQ=255)

                                        tCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATgc                                                    <  2:6036235/93‑1 (MQ=255)

                                        tCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATgc                                                    >  2:3424732/1‑93 (MQ=255)

                                         cGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATgcg                                                   <  1:2018730/93‑1 (MQ=255)

                                           gTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCa                                                 >  2:761242/1‑93 (MQ=255)

                                           gTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCa                                                 >  1:322177/1‑93 (MQ=255)

                                             aTGATTTTTTATTGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAgg                                               <  1:5647119/93‑1 (MQ=255)

                                             aTGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAgg                                               >  1:7153961/1‑93 (MQ=255)

                                               gAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGac                                             <  2:3632300/93‑1 (MQ=255)

                                               gAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGac                                             >  2:1265902/1‑93 (MQ=255)

                                                aGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGaca                                            >  2:2975646/1‑93 (MQ=255)

                                                 tttttttttGTCAAAGACGTTACCCAAGGCGCC‑GGCCCGCGTCCCGCCCCCCCAGAAATTGATGGAAAGCGTATGGGAAGATCCGCAGGacac                                           <  2:4939978/92‑1 (MQ=255)

                                                 gTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGCTAGCGTATGGGAAGATGCGCAGGacac                                           <  2:6423992/93‑1 (MQ=255)

                                                 gTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGacac                                           >  1:4089775/1‑93 (MQ=255)

                                                 gTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGacac                                           >  1:7068521/1‑93 (MQ=255)

                                                  tttttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACAcc                                          <  2:7239017/93‑1 (MQ=255)

                                                  tttttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACAcc                                          >  2:7182012/1‑93 (MQ=255)

                                                  tttttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACAcc                                          <  1:3339787/93‑1 (MQ=255)

                                                   ttttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCt                                         <  1:115319/93‑1 (MQ=255)

                                                   ttttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCt                                         <  2:2498470/93‑1 (MQ=255)

                                                    tttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTa                                        <  2:6140042/93‑1 (MQ=255)

                                                    tttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTa                                        <  2:3056824/93‑1 (MQ=255)

                                                     ttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTAc                                       <  2:5923359/93‑1 (MQ=255)

                                                     ttATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTAc                                       >  1:3646161/1‑93 (MQ=255)

                                                       aTTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGa                                     <  1:1700050/93‑1 (MQ=255)

                                                       aTTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGa                                     >  2:4150632/1‑93 (MQ=255)

                                                        ttgttgAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGAt                                    <  1:2594284/93‑1 (MQ=255)

                                                        ttgttgAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGAt                                    >  2:1681665/1‑93 (MQ=255)

                                                        ttgttgAAGACGTTACTCAAGCCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGAt                                    <  1:6290543/93‑1 (MQ=255)

                                                         tgttgAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATc                                   <  1:801904/93‑1 (MQ=255)

                                                         tgttgAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATc                                   <  2:3105380/93‑1 (MQ=255)

                                                          gttgAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCg                                  >  2:953251/1‑93 (MQ=255)

                                                             gAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCAc                               <  2:4871366/93‑1 (MQ=255)

                                                             gAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCAc                               <  2:1292136/93‑1 (MQ=255)

                                                             gAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCAc                               <  2:2808492/93‑1 (MQ=255)

                                                             gAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCAc                               >  2:5541761/1‑93 (MQ=255)

                                                             gAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCAc                               >  2:3766892/1‑93 (MQ=255)

                                                              aaGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCAcc                              <  1:4888079/93‑1 (MQ=255)

                                                              aaGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCAcc                              >  2:5141046/1‑93 (MQ=255)

                                                                 aCGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTc                           <  1:1473310/93‑1 (MQ=255)

                                                                 aCGTTACTCAAGTCACCGGGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGt                            <  1:2372540/93‑1 (MQ=255)

                                                                   gTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGa                         <  1:7218729/93‑1 (MQ=255)

                                                                        tCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATAccc                    >  2:4337119/1‑93 (MQ=255)

                                                                          aaGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCcac                  >  2:117978/1‑93 (MQ=255)

                                                                          aaGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCcac                  >  1:3801384/1‑93 (MQ=255)

                                                                            nncACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACAt                <  2:1981777/91‑1 (MQ=255)

                                                                            gccccc‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACAt                <  1:2472906/89‑1 (MQ=255)

                                                                            gTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACAt                >  1:4939307/1‑93 (MQ=255)

                                                                             tCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACAtt               >  2:445607/1‑93 (MQ=255)

                                                                             tCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACAtt               >  2:7493750/1‑93 (MQ=255)

                                                                               aCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTaa             >  1:4464770/1‑93 (MQ=255)

                                                                                ccggggCCGCGCGTGGTACCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTaa             <  1:5494444/90‑1 (MQ=255)

                                                                                cc‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTaaa            <  2:132567/93‑1 (MQ=255)

                                                                                   gggCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAAc          >  1:2876055/1‑93 (MQ=255)

                                                                                   gggCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAAc          >  2:797603/1‑93 (MQ=255)

                                                                                     gCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGc        <  1:4234728/93‑1 (MQ=255)

                                                                                     gCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGc        >  2:1543691/1‑93 (MQ=255)

                                                                                        gcgcCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGc     <  2:1798311/93‑1 (MQ=255)

                                                                                        gcgcCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGc     >  1:6739307/1‑93 (MQ=255)

                                                                                         cgTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCg    <  1:4344085/93‑1 (MQ=255)

                                                                                          gTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGt   >  2:5380418/1‑93 (MQ=255)

CGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTG  >  NC_000913/4634003‑4634185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGC  >  NC_000913/4634000‑4634186

CCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGC                                                                                               >  SRR4302542.329481/1‑94 (MQ=60)

  GCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCT                                                                                             <  SRR4302542.165928/94‑1 (MQ=60)

  GCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCT                                                                                             <  SRR4302542.7222674/94‑1 (MQ=60)

   CGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTG                                                                                            >  SRR4302542.3007903/1‑94 (MQ=60)

    GGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGG                                                                                           <  SRR4302542.2148395/94‑1 (MQ=60)

    GGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGG                                                                                           <  SRR4302542.7183957/94‑1 (MQ=60)

      CGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTC                                                                                         >  SRR4302542.4672640/1‑94 (MQ=60)

      CGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTC                                                                                         >  SRR4302542.2011170/1‑94 (MQ=60)

      CGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTC                                                                                         >  SRR4302542.3024526/1‑94 (MQ=60)

        CAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCC                                                                                       <  SRR4302542.5830320/94‑1 (MQ=60)

        CAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCC                                                                                       >  SRR4302542.4424152/1‑94 (MQ=60)

         AGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCG                                                                                      >  SRR4302542.5418269/1‑94 (MQ=60)

         AGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCG                                                                                      >  SRR4302542.7292081/1‑94 (MQ=60)

           ATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCC                                                                                    <  SRR4302542.4420971/94‑1 (MQ=60)

           ATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCACCTGGTCCCGCC                                                                                    >  SRR4302542.173519/1‑94 (MQ=60)

            TCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCA                                                                                   >  SRR4302542.3382282/1‑94 (MQ=60)

            TCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCA                                                                                   <  SRR4302542.7179936/94‑1 (MQ=60)

            TCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCA                                                                                   <  SRR4302542.2166056/94‑1 (MQ=60)

             CGGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAG                                                                                  <  SRR4302542.4655315/94‑1 (MQ=60)

              GGCTGTTTTGACACGCCATTAGCGCTGCCTCGGTATGAGTTTTTATTGTTAAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGC                                                                                 <  SRR4302542.6480212/94‑1 (MQ=60)

              AGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGC                                                                                 >  SRR4302542.6435445/1‑94 (MQ=60)

              AGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGC                                                                                 >  SRR4302542.3764332/1‑94 (MQ=60)

              AGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGC                                                                                 >  SRR4302542.2071805/1‑94 (MQ=60)

              AGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGC                                                                                 >  SRR4302542.5771265/1‑94 (MQ=60)

                CTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAA                                                                               >  SRR4302542.3175965/1‑94 (MQ=60)

                CTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAA                                                                               >  SRR4302542.4367633/1‑94 (MQ=60)

                     TTGACACGCCATTAGCGCTGCCTCGGTATGATTTTTTATTGTTGAAGCCTTTCCTAAAGCCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGAT                                                                          <  SRR4302542.2540004/94‑1 (MQ=53)

                      TGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATG                                                                         <  SRR4302542.7409193/94‑1 (MQ=60)

                      TGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATG                                                                         <  SRR4302542.7498251/94‑1 (MQ=60)

                       GACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGG                                                                        >  SRR4302542.949983/1‑94 (MQ=60)

                         CACGCCATTAGCGCTGCCTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGAT                                                                      <  SRR4302542.216165/94‑1 (MQ=60)

                         CACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGAT                                                                      >  SRR4302542.5334456/1‑94 (MQ=60)

                         CACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGAT                                                                      >  SRR4302542.2533171/1‑94 (MQ=60)

                         CACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGAT                                                                      <  SRR4302542.6009757/94‑1 (MQ=60)

                          ACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATA                                                                     <  SRR4302542.2000886/94‑1 (MQ=60)

                          ACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATA                                                                     <  SRR4302542.2079256/94‑1 (MQ=60)

                          ACGCCATTAGCCCTGACTCGGTATGATTTTTTATTGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATA                                                                     <  SRR4302542.6363897/94‑1 (MQ=60)

                           CGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAG                                                                    <  SRR4302542.3371067/94‑1 (MQ=60)

                            CCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGC                                                                   <  SRR4302542.296846/94‑1 (MQ=60)

                              CATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGT                                                                 >  SRR4302542.2454993/1‑94 (MQ=60)

                              CATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGT                                                                 >  SRR4302542.7063985/1‑94 (MQ=60)

                               ATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTA                                                                <  SRR4302542.2232130/94‑1 (MQ=60)

                               ATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTA                                                                >  SRR4302542.1294987/1‑94 (MQ=60)

                               ATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTAACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTA                                                                >  SRR4302542.7373772/1‑94 (MQ=60)

                                TTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTAT                                                               <  SRR4302542.4273449/94‑1 (MQ=60)

                                TTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTAT                                                               <  SRR4302542.6829637/94‑1 (MQ=60)

                                TTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTAT                                                               <  SRR4302542.7260664/94‑1 (MQ=60)

                                TTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTAT                                                               <  SRR4302542.1446698/94‑1 (MQ=60)

                                 TGGCGCTGCCTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATG                                                              <  SRR4302542.3849637/94‑1 (MQ=60)

                                  AGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGG                                                             >  SRR4302542.1889463/1‑94 (MQ=60)

                                  AGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGG                                                             >  SRR4302542.3411900/1‑94 (MQ=60)

                                     GCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAA                                                          >  SRR4302542.5724279/1‑94 (MQ=60)

                                      CTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAG                                                         >  SRR4302542.2489372/1‑94 (MQ=60)

                                       TGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGA                                                        >  SRR4302542.143595/1‑94 (MQ=60)

                                        GACTGGGTATGATTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAT                                                       <  SRR4302542.5575892/94‑1 (MQ=60)

                                        GACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAT                                                       <  SRR4302542.6244572/94‑1 (MQ=60)

                                        GACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAT                                                       >  SRR4302542.5449528/1‑94 (MQ=60)

                                        GACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAT                                                       <  SRR4302542.2953464/94‑1 (MQ=60)

                                        GACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGAT                                                       <  SRR4302542.7509655/94‑1 (MQ=60)

                                         ACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATG                                                      >  SRR4302542.4940329/1‑94 (MQ=60)

                                          CTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGC                                                     >  SRR4302542.1726694/1‑94 (MQ=60)

                                           tgggtatggtTTTTTATTTTTGAAGACGTTCCTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCG                                                    <  SRR4302542.2795083/84‑1 (MQ=60)

                                            CGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGC                                                   <  SRR4302542.2036191/94‑1 (MQ=60)

                                            CGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGC                                                   >  SRR4302542.2951491/1‑94 (MQ=60)

                                             GGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCA                                                  >  SRR4302542.324974/1‑94 (MQ=60)

                                             GGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCA                                                  <  SRR4302542.4894912/94‑1 (MQ=60)

                                              GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAG                                                 <  SRR4302542.1799640/94‑1 (MQ=60)

                                              GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAG                                                 >  SRR4302542.5827118/1‑94 (MQ=60)

                                              GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAG                                                 >  SRR4302542.5884386/1‑94 (MQ=60)

                                               TATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGG                                                <  SRR4302542.6112541/94‑1 (MQ=60)

                                               TATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGG                                                >  SRR4302542.7224144/1‑94 (MQ=60)

                                                ATGATTTTTTATTGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGA                                               <  SRR4302542.5701959/94‑1 (MQ=60)

                                                 TGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAAGTGATGGATAGCGTATGGGAAGATGCGCAGGAC                                              <  SRR4302542.4536709/94‑1 (MQ=60)

                                                 TGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGAC                                              <  SRR4302542.4548568/94‑1 (MQ=60)

                                                 TGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGAC                                              <  SRR4302542.7270820/94‑1 (MQ=60)

                                                  GAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACA                                             >  SRR4302542.2793836/1‑94 (MQ=60)

                                                  GAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGAGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACA                                             <  SRR4302542.2432566/94‑1 (MQ=60)

                                                   AGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACAC                                            >  SRR4302542.4130115/1‑94 (MQ=60)

                                                   AGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACAC                                            >  SRR4302542.2866778/1‑94 (MQ=60)

                                                   AGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACAC                                            >  SRR4302542.7137898/1‑94 (MQ=60)

                                                     TTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCT                                          <  SRR4302542.3830749/94‑1 (MQ=60)

                                                     TTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCT                                          <  SRR4302542.3371220/94‑1 (MQ=60)

                                                     TTTTTATTGTTAAAGACTTTGCTAAAGTCCCG‑GGGCCGGGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCT                                          <  SRR4302542.3947295/94‑1 (MQ=60)

                                                      TTTTATTGTTGAAGGCGTTCCTCAAGTCACG‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTA                                         <  SRR4302542.7423090/94‑1 (MQ=60)

                                                      TTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTA                                         <  SRR4302542.116270/94‑1 (MQ=60)

                                                       TTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTAC                                        <  SRR4302542.2312916/94‑1 (MQ=60)

                                                       TTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTAC                                        >  SRR4302542.3680194/1‑94 (MQ=60)

                                                        TTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACG                                       <  SRR4302542.1628038/94‑1 (MQ=60)

                                                         TATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGA                                      >  SRR4302542.5242551/1‑94 (MQ=60)

                                                          ATTGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGCATGGGAAGATGCGCAGGACACCTACGAT                                     <  SRR4302542.711649/94‑1 (MQ=60)

                                                          ATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGAT                                     <  SRR4302542.1714783/94‑1 (MQ=60)

                                                          ATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGAT                                     >  SRR4302542.6268931/1‑94 (MQ=60)

                                                          ATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGAT                                     >  SRR4302542.4214611/1‑94 (MQ=60)

                                                           TTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATC                                    <  SRR4302542.2619406/94‑1 (MQ=60)

                                                           TTGTTGAAGACGTTACTCAAGCCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATC                                    <  SRR4302542.6352521/94‑1 (MQ=60)

                                                            TGTTGAAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCG                                   <  SRR4302542.4094730/94‑1 (MQ=60)

                                                            TGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCG                                   <  SRR4302542.808806/94‑1 (MQ=60)

                                                             GTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGC                                  >  SRR4302542.766769/1‑94 (MQ=60)

                                                              gtggaagcctttcctaaagccccgGGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGC                                  <  SRR4302542.3712547/70‑1 (MQ=60)

                                                                 AAGACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCG                              <  SRR4302542.4935630/94‑1 (MQ=60)

                                                                 AAGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCG                              <  SRR4302542.3842497/94‑1 (MQ=60)

                                                                  AGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCAT                             >  SRR4302542.2393742/1‑94 (MQ=60)

                                                                  AGACGTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGT                             >  SRR4302542.1325235/1‑94 (MQ=60)

                                                                   GACTTTCCTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTC                            <  SRR4302542.5482175/94‑1 (MQ=60)

                                                                    ACGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCG                           <  SRR4302542.1486029/94‑1 (MQ=60)

                                                                    ACGTTACTCAAGTCACCGGGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTC                            <  SRR4302542.2393476/94‑1 (MQ=60)

                                                                     CGTTACTCAAGTCCCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGA                          <  SRR4302542.1877306/94‑1 (MQ=60)

                                                                      GTTACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGAT                         <  SRR4302542.7289544/94‑1 (MQ=60)

                                                                         ACTCAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACC                      >  SRR4302542.297232/1‑94 (MQ=60)

                                                                         ACTCAAGTCACC‑GGGCCGCGCCCGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACC                      >  SRR4302542.6571099/1‑94 (MQ=60)

                                                                          CCCAACTCACC‑GGGCCGCGCCTGTTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCC                     <  SRR4302542.3635835/94‑1 (MQ=60)

                                                                           TCAAGTCTCC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCA                    <  SRR4302542.6189199/94‑1 (MQ=60)

                                                                            CAAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCAC                   >  SRR4302542.3839011/1‑94 (MQ=60)

                                                                             AAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACA                  <  SRR4302542.3383783/94‑1 (MQ=60)

                                                                             AAGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACA                  >  SRR4302542.5773143/1‑94 (MQ=60)

                                                                              AGTCACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACAT                 >  SRR4302542.4987311/1‑94 (MQ=60)

                                                                               gcccCC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATT                <  SRR4302542.2496903/90‑1 (MQ=60)

                                                                                 CACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAA              >  SRR4302542.5970077/1‑94 (MQ=60)

                                                                                 CACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAA              >  SRR4302542.4508560/1‑94 (MQ=60)

                                                                                 CACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAA              >  SRR4302542.6816467/1‑94 (MQ=60)

                                                                                  ACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAA             >  SRR4302542.1313507/1‑94 (MQ=60)

                                                                                  ACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAA             <  SRR4302542.5547813/94‑1 (MQ=60)

                                                                                  ACC‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAA             <  SRR4302542.6608836/94‑1 (MQ=60)

                                                                                   CC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAA            <  SRR4302542.1204161/94‑1 (MQ=60)

                                                                                    C‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAAC           >  SRR4302542.2946005/1‑94 (MQ=60)

                                                                                    C‑GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAAC           >  SRR4302542.2903669/1‑94 (MQ=60)

                                                                                      GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACG          >  SRR4302542.3433689/1‑94 (MQ=60)

                                                                                      GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACG          >  SRR4302542.896205/1‑94 (MQ=60)

                                                                                      GGGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACG          >  SRR4302542.1727598/1‑94 (MQ=60)

                                                                                       GGCCGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGC         >  SRR4302542.1445361/1‑94 (MQ=60)

                                                                                        GCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCT        <  SRR4302542.4276431/94‑1 (MQ=60)

                                                                                        GCCGCGCCTGGCCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCT        <  SRR4302542.2708614/94‑1 (MQ=60)

                                                                                          CGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGC      <  SRR4302542.920891/94‑1 (MQ=60)

                                                                                          CGCGCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGC      >  SRR4302542.6805640/1‑94 (MQ=60)

                                                                                            CGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGT    <  SRR4302542.4386765/94‑1 (MQ=60)

                                                                                             GCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTG   <  SRR4302542.4691409/94‑1 (MQ=60)

                                                                                             GCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTG   <  SRR4302542.4770537/94‑1 (MQ=60)

                                                                                             GCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTG   >  SRR4302542.727030/1‑94 (MQ=60)

                                                                                             GCCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTG   <  SRR4302542.5673754/94‑1 (MQ=60)

                                                                                              CCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGC  >  SRR4302542.4992619/1‑94 (MQ=60)

CCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACC‑GGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGC  >  NC_000913/4634000‑4634186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`