Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,222,944 4 bp→AGCA coding (2671‑2674/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,222,9440CA99.8% 1828.3 / NA 472Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (229/242);  total (229/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,222,9450AG99.8% 1788.0 / NA 472Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (229/241);  total (229/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*NC_0009133,222,9460AC99.8% 1789.9 / NA 472Q891S (CAA→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (229/242);  minor base T (0/1);  total (229/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,222,9470GA98.3% 1712.5 / NA 471A892T (GCC→ACC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (228/234);  minor base C (0/8);  total (228/243)
Fisher's exact test for biased strand distribution p-value = 7.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

TAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAAC  >  NC_000913/3222850‑3223030
                                                                                              ||||                                                                                   
          tACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGTCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGAt                                                                             >  1:12104540/1‑96 (MQ=255)
          tACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGAt                                                                             <  1:2162814/96‑1 (MQ=255)
            cGATCAGGTGGCGTATTACGGTCGTGGACCGGGGGAAGACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGAGCAGCACCGTCGATGc                                                                           >  1:12822976/1‑96 (MQ=255)
            cGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGc                                                                           <  1:11582504/96‑1 (MQ=255)
             gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGATAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                          <  2:11768327/96‑1 (MQ=255)
             gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                          <  2:7215058/96‑1 (MQ=255)
             gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                          <  2:6066624/96‑1 (MQ=255)
             gATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGcc                                                                          >  1:2437393/1‑96 (MQ=255)
              aTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCa                                                                         >  1:6757572/1‑96 (MQ=255)
                cAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATg                                                                       <  1:4236448/96‑1 (MQ=255)
                 aGGTGGCGTATTACGGTCGTGGACCGGTCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                      <  2:12531865/96‑1 (MQ=255)
                 aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                      >  2:7686947/1‑96 (MQ=255)
                 aGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGt                                                                      <  2:2338450/96‑1 (MQ=255)
                  ggTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGtt                                                                     <  1:7075990/96‑1 (MQ=255)
                   gTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTc                                                                    >  1:594421/1‑96 (MQ=255)
                    tGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCg                                                                   <  2:6625383/96‑1 (MQ=255)
                    tGGCGTATTACGGTCGGGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCg                                                                   >  1:1891040/1‑96 (MQ=255)
                        gTATTACGGTCGTGGACCGGGCGCAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                               <  1:10137713/96‑1 (MQ=255)
                        gTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGaa                                                               >  1:453790/1‑96 (MQ=255)
                         tATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAAc                                                              <  1:2715612/96‑1 (MQ=255)
                          aTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAGCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACt                                                             >  2:11933863/1‑96 (MQ=255)
                          aTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCCCCGTCGATGCCATGTTCGAGAACt                                                             <  2:315107/96‑1 (MQ=255)
                          aTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACt                                                             >  2:8197953/1‑96 (MQ=255)
                           ttACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTa                                                            >  1:5031240/1‑96 (MQ=255)
                            tACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAt                                                           <  2:8010915/96‑1 (MQ=255)
                             aCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATc                                                          >  2:6469431/1‑96 (MQ=255)
                              cGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATcc                                                         <  1:403588/96‑1 (MQ=255)
                               ggTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATccc                                                        >  2:2835481/1‑96 (MQ=255)
                                gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCATCGTCGATGCCATGTTCGAGAACTATctct                                                       >  1:9149168/1‑96 (MQ=255)
                                gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                       >  1:1975953/1‑96 (MQ=255)
                                gTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCt                                                       <  1:6458242/96‑1 (MQ=255)
                                ctCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCCTGTTCGAGAACTATCCCt                                                       <  1:3150750/95‑1 (MQ=255)
                                 tCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                      <  2:9850360/96‑1 (MQ=255)
                                 tCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCtt                                                      <  1:5593862/96‑1 (MQ=255)
                                  cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc                                                     >  1:13523099/1‑96 (MQ=255)
                                  cGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTc                                                     <  1:8747704/96‑1 (MQ=255)
                                   gTGGACCGGGCGAAAACTCCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTcc                                                    <  2:2899709/96‑1 (MQ=255)
                                    tGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTccc                                                   >  1:7310062/1‑96 (MQ=255)
                                     ggACCGGGCGATAACTACGCCGCCAGCCAGCAGGCTATCCTCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCg                                                  <  1:14331311/96‑1 (MQ=255)
                                     ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCg                                                  <  1:5163260/96‑1 (MQ=255)
                                     ggACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCg                                                  >  1:9004201/1‑96 (MQ=255)
                                      gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGc                                                 >  2:1124693/1‑96 (MQ=255)
                                      gACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGc                                                 <  2:872115/96‑1 (MQ=255)
                                       aCCGGCCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCa                                                <  2:4064097/96‑1 (MQ=255)
                                         cGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGa                                              >  2:10278678/1‑96 (MQ=255)
                                          gggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaa                                             <  1:3611480/96‑1 (MQ=255)
                                          gggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaa                                             >  2:5255145/1‑96 (MQ=255)
                                           ggCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaac                                            <  2:7343001/96‑1 (MQ=255)
                                            gCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaaca                                           <  2:5670054/96‑1 (MQ=255)
                                             cGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaa                                          >  1:10999077/1‑96 (MQ=255)
                                              gAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGaacaac                                         >  1:7961899/1‑96 (MQ=255)
                                               aaaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCCCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                        <  2:5015697/96‑1 (MQ=255)
                                               aaaaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACg                                        <  1:4936343/96‑1 (MQ=255)
                                                 aaCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGt                                      <  1:7357058/96‑1 (MQ=255)
                                                  aCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTa                                     >  2:12310178/1‑96 (MQ=255)
                                                   cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGtga                                    <  1:11121198/96‑3 (MQ=255)
                                                   cTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTaa                                    <  1:6905014/96‑1 (MQ=255)
                                                    tACGGCGACAGCCAGCAGGCTAACATCAACGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                                   <  2:3655834/96‑1 (MQ=255)
                                                    tACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAc                                   <  1:8294434/96‑1 (MQ=255)
                                                     aCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGTACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAcc                                  <  1:9340065/96‑1 (MQ=255)
                                                      cGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCg                                 >  2:13801870/1‑96 (MQ=255)
                                                       gCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGt                                >  2:409484/1‑96 (MQ=255)
                                                         cGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCa                              >  1:9691547/1‑96 (MQ=255)
                                                          gACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAg                             >  1:13219458/1‑96 (MQ=255)
                                                            cagccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                           >  2:11455158/1‑96 (MQ=255)
                                                            cagccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCa                           >  2:13300930/1‑96 (MQ=255)
                                                             agccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTACCCGTCAGcct                          <  1:10828334/96‑3 (MQ=255)
                                                             agccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCTTCAGCAt                          >  1:634643/1‑96 (MQ=255)
                                                             agccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAt                          >  1:7736274/1‑96 (MQ=255)
                                                              gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                         <  2:7451040/96‑1 (MQ=255)
                                                              gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                         <  1:10006881/96‑1 (MQ=255)
                                                              gccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATg                         >  2:8579441/1‑96 (MQ=255)
                                                               ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt                        >  2:10511932/1‑96 (MQ=255)
                                                               ccagcAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGt                        <  1:7826186/96‑1 (MQ=255)
                                                                cagcagGCTAACATCATCGATATCTGGCGCAGCCCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                       <  2:9471920/96‑1 (MQ=255)
                                                                cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAGCGGTAACCGTCAGCATGTc                       >  2:6801896/1‑96 (MQ=255)
                                                                cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                       >  1:1592339/1‑96 (MQ=255)
                                                                cagcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTc                       >  2:4787411/1‑96 (MQ=255)
                                                                 agcagGCTAACATCATCGATATCTGGCGCATCACCGTCGATGCCATGTTCGAGAACTATCCCATCCCGCAGAACAACGGTAACCGTCAGCACGTcc                      <  2:14087766/96‑1 (MQ=255)
                                                                 agcagGCTAACATCATCGATATCTGGCGCAGCACCGTTGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGttc                      <  1:10652932/96‑3 (MQ=255)
                                                                 agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                      <  2:12058981/96‑1 (MQ=255)
                                                                 agcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTcc                      >  2:9595163/1‑96 (MQ=255)
                                                                  gcagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGGTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCg                     >  2:6349641/1‑96 (MQ=255)
                                                                   cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                    >  2:10894585/1‑96 (MQ=255)
                                                                   cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                    >  2:12989173/1‑96 (MQ=255)
                                                                   cagGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGc                    <  2:437132/96‑1 (MQ=255)
                                                                    agGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCt                   >  1:10396360/1‑96 (MQ=255)
                                                                     ggCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTg                  <  1:11997151/96‑1 (MQ=255)
                                                                      gCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTgg                 <  2:2720184/96‑1 (MQ=255)
                                                                       cTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGa                <  1:9964191/96‑1 (MQ=255)
                                                                         aaCATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACg              <  1:8617178/96‑1 (MQ=255)
                                                                           catcatCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGc            >  2:3451776/1‑96 (MQ=255)
                                                                            atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCa           <  1:3464458/96‑1 (MQ=255)
                                                                            atcatcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCa           <  2:8981097/96‑1 (MQ=255)
                                                                               atcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTg        >  1:12467537/1‑96 (MQ=255)
                                                                               atcGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCACGTCCGCTGGACGGCACTg        >  1:13974153/1‑96 (MQ=255)
                                                                                  gATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACt     >  2:13730858/1‑96 (MQ=255)
                                                                                  gATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACt     <  2:3967757/96‑1 (MQ=255)
                                                                                  gATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACt     >  1:4747810/1‑96 (MQ=255)
                                                                                   atatCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTa    >  2:1533644/1‑96 (MQ=255)
                                                                                              ||||                                                                                   
TAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCCAAGCCGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAAC  >  NC_000913/3222850‑3223030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 249 total aligned reads displayed.
GGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAG‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCC  >  NC_000913/3222844‑3223034
                                                                                                      |                                                                                              
GGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCNN‑‑N                                                                                                >  SRR4302518.9143994/1‑101 (MQ=60)
 NNNATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CG                                                                                               <  SRR4302518.10771054/101‑1 (MQ=60)
  AAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑NNN                                                                                              >  SRR4302518.11803499/1‑101 (MQ=60)
             NNNTACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGC                                                                               <  SRR4302518.12127874/101‑1 (MQ=60)
             CAATACCATCAGGTGGCGTAGTACGGTCGTGGACCGGGCGAAAACTACGCCGCCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGC                                                                               <  SRR4302518.1942795/101‑1 (MQ=60)
              NNNACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCC                                                                              <  SRR4302518.11403609/101‑1 (MQ=60)
              NNNACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCC                                                                              <  SRR4302518.8250739/101‑1 (MQ=60)
              NNNACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCC                                                                              <  SRR4302518.7588571/101‑1 (MQ=60)
              AATACGATCAGGGGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCC                                                                              >  SRR4302518.6274822/1‑101 (MQ=60)
               ATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCA                                                                             >  SRR4302518.3172862/1‑101 (MQ=60)
               ATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCA                                                                             >  SRR4302518.4999416/1‑101 (MQ=60)
                TACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCAT                                                                            >  SRR4302518.4030633/1‑101 (MQ=60)
                NNNGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCAT                                                                            <  SRR4302518.10522924/101‑1 (MQ=60)
                 TCGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATG                                                                           <  SRR4302518.4444288/101‑1 (MQ=60)
                 NNNATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATG                                                                           <  SRR4302518.7707882/101‑1 (MQ=60)
                 ACGATCAGGTGGCGTATTGCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATG                                                                           >  SRR4302518.2037283/1‑101 (MQ=60)
                 ACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATG                                                                           <  SRR4302518.1111253/101‑1 (MQ=60)
                  NNNTCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGT                                                                          <  SRR4302518.11360693/101‑1 (MQ=60)
                  CGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGT                                                                          <  SRR4302518.7253120/101‑1 (MQ=60)
                   NNNCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTT                                                                         <  SRR4302518.7449790/101‑1 (MQ=60)
                   GATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTT                                                                         >  SRR4302518.2555131/1‑101 (MQ=60)
                   GATCAGGTGGCGTATTACGGGCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTT                                                                         >  SRR4302518.2745906/1‑101 (MQ=60)
                    NNNAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                        <  SRR4302518.10906799/101‑1 (MQ=60)
                    NNNAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                        <  SRR4302518.14139695/101‑1 (MQ=60)
                    NNNAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                        <  SRR4302518.9029739/101‑1 (MQ=60)
                    NNNAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTGC                                                                        <  SRR4302518.11557525/101‑1 (MQ=60)
                    ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAATCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                        <  SRR4302518.2205511/101‑1 (MQ=60)
                    ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                        >  SRR4302518.7110428/1‑101 (MQ=60)
                    ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑‑‑AGGAC‑‑CGTCGATGCCATGTTC                                                                        >  SRR4302518.7215409/1‑101 (MQ=60)
                     TCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCG                                                                       >  SRR4302518.2538501/1‑101 (MQ=60)
                     TCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCG                                                                       <  SRR4302518.1838795/101‑1 (MQ=60)
                     TAAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTNNN                                                                       >  SRR4302518.10077689/1‑101 (MQ=60)
                      CAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTNNN                                                                      >  SRR4302518.10346666/1‑101 (MQ=60)
                      CAGGGGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGTCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGA                                                                      >  SRR4302518.4643589/1‑101 (MQ=60)
                       AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCNNN                                                                     >  SRR4302518.7650097/1‑101 (MQ=60)
                        GGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGNNN                                                                    >  SRR4302518.7365584/1‑101 (MQ=60)
                        GGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTAGATGCCATGTTCGAGA                                                                    >  SRR4302518.7213594/1‑101 (MQ=60)
                         NNNGCGTATTACGGTCGTGGACCGGGCGCAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                                   <  SRR4302518.10616854/101‑1 (MQ=60)
                         NNNGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                                   <  SRR4302518.8540080/101‑1 (MQ=60)
                         GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGANNN                                                                   >  SRR4302518.9171988/1‑101 (MQ=60)
                         GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                                   <  SRR4302518.1104853/101‑1 (MQ=60)
                         GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                                   >  SRR4302518.617506/1‑101 (MQ=60)
                         GTGGCGTATTACGGGCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCGGGCGCAGCA‑‑‑‑C‑‑CGCCGTTGCCATGTTCGAGAA                                                                   >  SRR4302518.4322182/1‑101 (MQ=60)
                          TGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAAC                                                                  >  SRR4302518.905772/1‑101 (MQ=60)
                          TGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑‑‑AGCCC‑‑CGTCGATGCCATGTTCGAGAAC                                                                  <  SRR4302518.3820797/101‑1 (MQ=60)
                          TGGCGTATTACGGTCGTGGACCGGGCGAAAACCACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAAC                                                                  <  SRR4302518.2844789/101‑1 (MQ=60)
                           GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACT                                                                 >  SRR4302518.6574918/1‑101 (MQ=60)
                              NNNTTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATC                                                              <  SRR4302518.14126923/101‑1 (MQ=60)
                               TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGCTATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTANNN                                                             >  SRR4302518.9428677/1‑101 (MQ=60)
                               TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCC                                                             <  SRR4302518.419379/101‑1 (MQ=60)
                               TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCC                                                             <  SRR4302518.2213577/101‑1 (MQ=60)
                               NNNTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCC                                                             <  SRR4302518.10694184/101‑1 (MQ=60)
                                ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATNNN                                                            >  SRR4302518.12733663/1‑101 (MQ=60)
                                ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCC                                                            <  SRR4302518.5131943/101‑1 (MQ=60)
                                 TTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCT                                                           <  SRR4302518.6788758/101‑1 (MQ=60)
                                 TTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCT                                                           >  SRR4302518.5292220/1‑101 (MQ=60)
                                 TTACGCTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCCTGTTCGAGAACTATCCCT                                                           <  SRR4302518.3298018/101‑1 (MQ=60)
                                  TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTT                                                          <  SRR4302518.6691938/101‑1 (MQ=60)
                                  TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTT                                                          <  SRR4302518.4285393/101‑1 (MQ=60)
                                  TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTT                                                          <  SRR4302518.5877609/101‑1 (MQ=60)
                                  NNNGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTT                                                          <  SRR4302518.11275708/101‑1 (MQ=60)
                                   NNNGTCGTTGACCGGGCGAAAAATACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTC                                                         <  SRR4302518.9077041/101‑1 (MQ=60)
                                   NNNGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTC                                                         <  SRR4302518.9172751/101‑1 (MQ=60)
                                   ATGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCTAGCACTATCTCTTC                                                         >  SRR4302518.1369850/1‑101 (MQ=60)
                                   ACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCNNN                                                         >  SRR4302518.7964859/1‑101 (MQ=60)
                                     NNNCGTGGACCGTGCGAAAACTACTCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                       <  SRR4302518.7729294/101‑1 (MQ=60)
                                     NNNCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                       <  SRR4302518.12815999/101‑1 (MQ=60)
                                     GGTCGTGGACCGGGCGAAACCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                       <  SRR4302518.4507172/101‑1 (MQ=60)
                                     GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGGACTATCCCTTNNN                                                       >  SRR4302518.14647844/1‑101 (MQ=60)
                                     GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTNNN                                                       >  SRR4302518.13916507/1‑101 (MQ=60)
                                     GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTNNN                                                       >  SRR4302518.8795032/1‑101 (MQ=60)
                                     GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                       >  SRR4302518.6103684/1‑101 (MQ=60)
                                     GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                       <  SRR4302518.5268748/101‑1 (MQ=60)
                                     GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                       <  SRR4302518.2184129/101‑1 (MQ=60)
                                     GGTCGTGGACCGGGCGAAAACCACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                       <  SRR4302518.3660741/101‑1 (MQ=60)
                                      nnngcGGACCGGGCGATAACTACGCCGCCAGCCAGCAGGCTATCCTCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCG                                                      <  SRR4302518.14994437/96‑1 (MQ=60)
                                      gtgagGGACCGGGCAAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑‑‑AGCCC‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCG                                                      <  SRR4302518.6991964/96‑1 (MQ=60)
                                      NNNGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCG                                                      <  SRR4302518.12627195/101‑1 (MQ=60)
                                      GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCNNN                                                      >  SRR4302518.8583461/1‑101 (MQ=60)
                                      GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCG                                                      >  SRR4302518.2066476/1‑101 (MQ=60)
                                      GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCG                                                      >  SRR4302518.3139950/1‑101 (MQ=60)
                                      GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCG                                                      <  SRR4302518.5429608/101‑1 (MQ=60)
                                      GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑‑‑AG‑‑CATCGTCGATGCCATGTTCGAGAACTATCtcttgnnn                                                      >  SRR4302518.9590498/1‑93 (MQ=60)
                                       TCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCNNN                                                     >  SRR4302518.12864244/1‑101 (MQ=60)
                                        NNNGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCA                                                    <  SRR4302518.14572456/101‑1 (MQ=60)
                                        CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCNNN                                                    >  SRR4302518.14143903/1‑101 (MQ=60)
                                         GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGNNN                                                   >  SRR4302518.10353480/1‑101 (MQ=60)
                                         GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                                   >  SRR4302518.480531/1‑101 (MQ=60)
                                         GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                                   <  SRR4302518.817340/101‑1 (MQ=60)
                                         GAGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGNNN                                                   >  SRR4302518.12317319/1‑101 (MQ=60)
                                         CTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑‑‑AGCTC‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                                   <  SRR4302518.7322751/101‑1 (MQ=60)
                                          TGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCNNN                                                  >  SRR4302518.7690598/1‑101 (MQ=60)
                                          TGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGA                                                  >  SRR4302518.1234892/1‑101 (MQ=60)
                                           NNNCCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAA                                                 <  SRR4302518.10340310/101‑1 (MQ=60)
                                           GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCANNN                                                 >  SRR4302518.9439485/1‑101 (MQ=60)
                                           GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAA                                                 <  SRR4302518.3780710/101‑1 (MQ=60)
                                             NNNGGGCAAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACC                                               <  SRR4302518.11846653/101‑1 (MQ=60)
                                             CCCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                               <  SRR4302518.4880977/101‑1 (MQ=60)
                                             ACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                               >  SRR4302518.5314478/1‑101 (MQ=60)
                                             ACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                               <  SRR4302518.4765269/101‑1 (MQ=60)
                                               CGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTGTCCCTCCCCGCAGAACAAC                                             >  SRR4302518.1115803/1‑101 (MQ=60)
                                               CGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                             <  SRR4302518.578611/101‑1 (MQ=60)
                                                GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACANNN                                            >  SRR4302518.7835456/1‑101 (MQ=60)
                                                GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACANNN                                            >  SRR4302518.9312959/1‑101 (MQ=60)
                                                GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACG                                            <  SRR4302518.5193286/101‑1 (MQ=60)
                                                  NNNAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGT                                          <  SRR4302518.7738795/101‑1 (MQ=60)
                                                  GCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGT                                          <  SRR4302518.530988/101‑1 (MQ=60)
                                                  GCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGA                                          >  SRR4302518.5131616/1‑101 (MQ=60)
                                                   CGAAGACTACGCCGACAGCCAGCAGGCTAACATCATCGAAATCTGGCG‑‑‑‑AAGCAC‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGNNN                                         >  SRR4302518.13205467/1‑101 (MQ=60)
                                                   CGAAAACTACGCCGCCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTA                                         <  SRR4302518.3520151/101‑1 (MQ=60)
                                                   CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGNNN                                         >  SRR4302518.11514079/1‑101 (MQ=60)
                                                   CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTA                                         <  SRR4302518.6701018/101‑1 (MQ=60)
                                                   CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTA                                         >  SRR4302518.1879259/1‑101 (MQ=60)
                                                   CCAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CGCA‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTA                                         <  SRR4302518.3184420/101‑1 (MQ=60)
                                                    NNNAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTGA                                        <  SRR4302518.11641382/101‑1 (MQ=60)
                                                    GTAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGNNN                                        >  SRR4302518.14988335/1‑101 (MQ=60)
                                                    GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAA                                        <  SRR4302518.7271348/101‑1 (MQ=60)
                                                    GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAA                                        >  SRR4302518.197655/1‑101 (MQ=60)
                                                    GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAA                                        <  SRR4302518.3755287/101‑1 (MQ=60)
                                                    GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGNNN                                        >  SRR4302518.8359884/1‑101 (MQ=60)
                                                     NNNACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                       <  SRR4302518.9824121/101‑1 (MQ=60)
                                                     NNNACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                       <  SRR4302518.8702955/101‑1 (MQ=60)
                                                     AAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                       >  SRR4302518.5235166/1‑101 (MQ=60)
                                                      NNNCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑‑‑AGTAC‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                                      <  SRR4302518.9791486/101‑1 (MQ=60)
                                                      AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                                      >  SRR4302518.2364820/1‑101 (MQ=60)
                                                        cccaccCCGACCGCCAGCCGGCTAACATCATCGATACCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACCATCCCTTCCCGCAGAACAACGGTAACCGT                                    <  SRR4302518.3972435/95‑1 (MQ=60)
                                                        ACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGT                                    <  SRR4302518.6659216/101‑1 (MQ=60)
                                                          TGCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGNNN                                  >  SRR4302518.8647610/1‑101 (MQ=60)
                                                          TACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCA                                  >  SRR4302518.6719508/1‑101 (MQ=60)
                                                          TACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGNNN                                  >  SRR4302518.7356749/1‑101 (MQ=60)
                                                          NNNGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCA                                  <  SRR4302518.10504984/101‑1 (MQ=60)
                                                           ACGCGGACAGCCAGCAGGGTAACATCATCGATATCTGGCTCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTNNN                                 >  SRR4302518.13205251/1‑101 (MQ=60)
                                                            NNNCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGC                                <  SRR4302518.10498866/101‑1 (MQ=60)
                                                            CGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCNNN                                >  SRR4302518.14967608/1‑101 (MQ=60)
                                                             GCCGACAGCCAGCAGGCTAAAATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCA                               >  SRR4302518.2812744/1‑101 (MQ=60)
                                                              NNNACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTACCCGTCAGCCT                              <  SRR4302518.11336767/101‑1 (MQ=60)
                                                              NNNACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGCTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCCT                              <  SRR4302518.13644898/101‑1 (MQ=60)
                                                               NNNCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                             <  SRR4302518.8188713/101‑1 (MQ=60)
                                                               NNNCAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                             <  SRR4302518.10482564/101‑1 (MQ=60)
                                                               CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCNNN                             >  SRR4302518.10158033/1‑101 (MQ=60)
                                                               CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCNNN                             >  SRR4302518.9680897/1‑101 (MQ=60)
                                                               CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                             >  SRR4302518.6431719/1‑101 (MQ=60)
                                                               CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                             >  SRR4302518.3459753/1‑101 (MQ=60)
                                                                NNNAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGT                            <  SRR4302518.8220424/101‑1 (MQ=60)
                                                                GACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCANNN                            >  SRR4302518.13829183/1‑101 (MQ=60)
                                                                GACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCANNN                            >  SRR4302518.8236511/1‑101 (MQ=60)
                                                                GACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCANNN                            >  SRR4302518.7714778/1‑101 (MQ=60)
                                                                 ACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTC                           >  SRR4302518.1534815/1‑101 (MQ=60)
                                                                  NNNCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTTGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTTC                          <  SRR4302518.11153578/101‑1 (MQ=60)
                                                                  CAGCCAGCAGGCTAACCTCATCGCTATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATCTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                          <  SRR4302518.2114962/101‑1 (MQ=60)
                                                                  CAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGNNN                          >  SRR4302518.12633330/1‑101 (MQ=60)
                                                                  CAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGGAACCGTCAGCATGTCC                          >  SRR4302518.4555091/1‑101 (MQ=60)
                                                                   NNNCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG                         <  SRR4302518.9377385/101‑1 (MQ=60)
                                                                   AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCTTCAGCATCTCCG                         >  SRR4302518.659298/1‑101 (MQ=60)
                                                                   AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTNNN                         >  SRR4302518.8128287/1‑101 (MQ=60)
                                                                   AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAAAACGATAACCGTCAGCATGTACG                         >  SRR4302518.5611330/1‑101 (MQ=60)
                                                                    NNNAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGC                        <  SRR4302518.12588841/101‑1 (MQ=60)
                                                                    GCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGC                        <  SRR4302518.6800570/101‑1 (MQ=60)
                                                                    GCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGC                        >  SRR4302518.939902/1‑101 (MQ=60)
                                                                     CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                       >  SRR4302518.4346805/1‑101 (MQ=60)
                                                                     CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                       >  SRR4302518.982426/1‑101 (MQ=60)
                                                                      NNNCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG                      <  SRR4302518.12568987/101‑1 (MQ=60)
                                                                      CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGNNN                      >  SRR4302518.9786709/1‑101 (MQ=60)
                                                                      CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG                      <  SRR4302518.2621359/101‑1 (MQ=60)
                                                                      CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG                      >  SRR4302518.1661762/1‑101 (MQ=60)
                                                                        GCAGGGTAACATCATCGCTATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTNNN                    >  SRR4302518.14588166/1‑101 (MQ=60)
                                                                        GCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA                    <  SRR4302518.2968500/101‑1 (MQ=60)
                                                                         CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGNNN                   >  SRR4302518.8857168/1‑101 (MQ=60)
                                                                          NNNCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACG                  <  SRR4302518.9036836/101‑1 (MQ=60)
                                                                          AGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGNNN                  >  SRR4302518.10884548/1‑101 (MQ=60)
                                                                           GGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG                 <  SRR4302518.6219669/101‑1 (MQ=60)
                                                                            NNNAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCTGCAGAACAACGGTAACCGTCGGCATGTCCGCTGGCCGGC                <  SRR4302518.13202081/101‑1 (MQ=60)
                                                                             NNNACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCA               <  SRR4302518.9722354/101‑1 (MQ=60)
                                                                             GTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCA               <  SRR4302518.3625690/101‑1 (MQ=60)
                                                                             CTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGTTAACCGTCAGCATGTCCGCTGGACGNNN               >  SRR4302518.10701607/1‑101 (MQ=60)
                                                                             CTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGGAACCGTGCGCATGTCCGCTGGACGNNN               >  SRR4302518.11783757/1‑101 (MQ=60)
                                                                              TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGNNN              >  SRR4302518.10955398/1‑101 (MQ=60)
                                                                              TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAC              >  SRR4302518.147939/1‑101 (MQ=60)
                                                                              TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGGCAGCATGTCCGCTGGCCGGCAC              >  SRR4302518.6962570/1‑101 (MQ=60)
                                                                               NNNATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACT             <  SRR4302518.14159406/101‑1 (MQ=60)
                                                                               AACATCATCGATATCTTGCGCAGCA‑‑‑‑C‑‑CGTCGATTCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACT             <  SRR4302518.4915443/101‑1 (MQ=60)
                                                                               AACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTACCCGTCAGCATGTCCGCTGGACGGCACT             <  SRR4302518.5313570/101‑1 (MQ=60)
                                                                               AACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCNNN             >  SRR4302518.9531341/1‑101 (MQ=60)
                                                                                AGATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCANNN            >  SRR4302518.8141826/1‑101 (MQ=60)
                                                                                    CATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGANNN        >  SRR4302518.11389828/1‑101 (MQ=60)
                                                                                    CATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTA        >  SRR4302518.1555197/1‑101 (MQ=60)
                                                                                     ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAA       <  SRR4302518.1977062/101‑1 (MQ=60)
                                                                                     ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAA       <  SRR4302518.561532/101‑1 (MQ=60)
                                                                                     ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAA       <  SRR4302518.5162900/101‑1 (MQ=60)
                                                                                     ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACNNN       >  SRR4302518.13054037/1‑101 (MQ=60)
                                                                                     ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCACGTCCGCTGGACGGCACTGACNNN       >  SRR4302518.14613650/1‑101 (MQ=60)
                                                                                     ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CATCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACNNN       >  SRR4302518.13472839/1‑101 (MQ=60)
                                                                                       CGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTANNN     >  SRR4302518.10970327/1‑101 (MQ=60)
                                                                                       CGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTANNN     >  SRR4302518.7570669/1‑101 (MQ=60)
                                                                                        GATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAANNN    >  SRR4302518.11591730/1‑101 (MQ=60)
                                                                                        GATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCG    >  SRR4302518.4996794/1‑101 (MQ=60)
                                                                                         ATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGC   >  SRR4302518.1238072/1‑101 (MQ=60)
                                                                                         ATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGC   <  SRR4302518.1403326/101‑1 (MQ=60)
                                                                                         ATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGGAACCGTCAGCATGTCCGCTGGACGGCACTGAgtaacgga   >  SRR4302518.2307714/1‑93 (MQ=60)
                                                                                          TATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCC  <  SRR4302518.1650703/101‑1 (MQ=60)
                                                                                                      |                                                                                              
GGGAATTAACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAG‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGCC  >  NC_000913/3222844‑3223034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑