Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,449,738 +G coding (1106/1254 nt) ytfR → YtfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,449,7371.G99.3% 1705.1 / 1.4 446coding (1105/1254 nt)ytfRYtfR
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (241/202);  total (243/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 483 total aligned reads displayed.

TTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC  >  NC_000913/4449642‑4449827
                                                                                                 |                                                                                          
ttGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t                                                                                             >  1:2890525/1‑96 (MQ=255)
  ggCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑gg                                                                                          <  2:14403549/96‑2 (MQ=255)
  ggCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑gg                                                                                          >  1:4504407/1‑95 (MQ=255)
    cTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑ggct                                                                                        <  2:10410184/96‑4 (MQ=255)
    cTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑ggct                                                                                        <  2:9877702/96‑4 (MQ=255)
     ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgc‑t‑ggcta                                                                                       <  2:2813253/96‑5 (MQ=255)
       cTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACg                                                                                      <  2:6929057/96‑1 (MQ=255)
         gCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTc                                                                                    >  2:3679458/1‑96 (MQ=255)
          cccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTcc                                                                                   >  2:13636733/1‑96 (MQ=255)
           ccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTccc                                                                                  >  2:2243619/1‑96 (MQ=255)
            cGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCa                                                                                 >  2:2398414/1‑96 (MQ=255)
             ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCAt                                                                                >  1:8346292/1‑96 (MQ=255)
             ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCAt                                                                                >  2:9261417/1‑96 (MQ=255)
                aGACAGGAAAACCAATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTc                                                                             <  2:10265906/96‑1 (MQ=255)
                 gACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTcc                                                                            >  1:3995855/1‑96 (MQ=255)
                  aCAGGAAAGCCGATGGCATCCTGGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCGGCGCGGC‑TGGCTACGTCCCCCTTccc                                                                           <  1:3345197/96‑1 (MQ=255)
                   cAGGAAAACCGATTGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCg                                                                          >  1:13900260/1‑96 (MQ=255)
                   cAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCg                                                                          <  2:9415059/96‑1 (MQ=255)
                   cAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCg                                                                          <  1:14136139/96‑1 (MQ=255)
                    aGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGc                                                                         <  2:9495198/96‑1 (MQ=255)
                    aGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGc                                                                         >  1:8714842/1‑96 (MQ=255)
                     ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCa                                                                        <  1:14401803/96‑1 (MQ=255)
                      gAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCaa                                                                       >  1:13385465/1‑96 (MQ=255)
                         aaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGa                                                                    >  1:2496551/1‑96 (MQ=255)
                         aaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGa                                                                    >  1:7840281/1‑96 (MQ=255)
                           ccGATGGCATCCTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAAc                                                                  <  1:11621213/96‑1 (MQ=255)
                           ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAAc                                                                  <  1:9726010/96‑1 (MQ=255)
                            cGATGGCATCGTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACa                                                                 <  1:7203000/96‑1 (MQ=255)
                             gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAg                                                                >  1:10031976/1‑96 (MQ=255)
                             gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAg                                                                >  1:3629581/1‑96 (MQ=255)
                             gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGGCCCATTTCCCGCAAAGAACAg                                                                >  2:6000674/1‑96 (MQ=255)
                               tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCa                                                              <  2:169350/96‑1 (MQ=255)
                                 gCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                            <  1:5339291/96‑1 (MQ=255)
                                    tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                         <  2:2976779/96‑1 (MQ=255)
                                     catcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAGGAACAGCAAGAGAt                                                        >  1:3076074/1‑96 (MQ=255)
                                     catcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                        >  2:10042583/1‑96 (MQ=255)
                                      atcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGGtt                                                       >  2:3406439/1‑96 (MQ=255)
                                       tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                      <  1:7614221/96‑1 (MQ=255)
                                        cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGc                                                     >  2:4720079/1‑96 (MQ=255)
                                         gCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGcc                                                    >  2:11352536/1‑96 (MQ=255)
                                           tGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGa                                                  >  2:10749722/1‑96 (MQ=255)
                                            gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                                 <  2:8905105/96‑1 (MQ=255)
                                            gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                                 >  2:7357525/1‑96 (MQ=255)
                                            gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAGTGCCGaa                                                 >  2:8422641/1‑96 (MQ=255)
                                             ccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGACTGCCGAAc                                                <  2:12956955/96‑1 (MQ=255)
                                             ccgccTCGGTGAGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCTGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                                <  1:12385585/96‑1 (MQ=255)
                                              cgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCAATTCCCGCAAAGAAGAGCAATATATTGTCGAACg                                               >  1:2795225/1‑96 (MQ=255)
                                              cgccTCGGTGCGGGAAAATATCATCCTCGCACTCCAGGCCTAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACg                                               <  2:11626403/96‑1 (MQ=255)
                                                ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCt                                             >  2:6344792/1‑96 (MQ=255)
                                                 cTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCtt                                            >  1:5002845/1‑96 (MQ=255)
                                                   cGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                          >  1:8989494/1‑96 (MQ=255)
                                                    ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAt                                         >  2:4188171/1‑96 (MQ=255)
                                                     gTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                        >  1:13008622/1‑96 (MQ=255)
                                                      tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                       <  1:6563845/96‑1 (MQ=255)
                                                      tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTGTATct                                       >  1:6841276/1‑95 (MQ=255)
                                                       gCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                      >  1:13288045/1‑96 (MQ=255)
                                                       gCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                      >  1:13632753/1‑96 (MQ=255)
                                                         gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCGGTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGGTTTATCCGcc                                    >  2:14012848/1‑96 (MQ=255)
                                                         gcgAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGcc                                    <  1:10406372/94‑1 (MQ=255)
                                                           gAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAg                                  >  2:4932842/1‑96 (MQ=255)
                                                           gAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAg                                  <  2:3795227/96‑1 (MQ=255)
                                                            aaaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGc                                 >  2:8963372/1‑96 (MQ=255)
                                                             aaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                                >  1:7500976/1‑96 (MQ=255)
                                                               atatCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTg                              <  1:6199437/96‑1 (MQ=255)
                                                                tatCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTgg                             >  2:7172895/1‑96 (MQ=255)
                                                                   catcCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAt                          >  2:7825467/1‑96 (MQ=255)
                                                                     tcCTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                        <  1:6310441/96‑1 (MQ=255)
                                                                      ccTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCg                       >  1:2220007/1‑96 (MQ=255)
                                                                       cTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                      <  1:5277161/96‑1 (MQ=255)
                                                                       cTCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                      >  2:7597770/1‑96 (MQ=255)
                                                                        tCGCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                     >  1:1803825/1‑96 (MQ=255)
                                                                          gCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcaca                   >  2:13878466/1‑96 (MQ=255)
                                                                          gCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcaca                   >  2:3966134/1‑96 (MQ=255)
                                                                          gCTCTCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcaca                   >  2:14078158/1‑96 (MQ=255)
                                                                           ctctCCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCacac                  <  1:11995655/96‑1 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGCGG‑GCTACGTCCCATTTCCCGCAAAGAACAACAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCCCACAcc                 >  2:12778040/1‑96 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc                 >  2:4416108/1‑96 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc                 >  1:2064102/1‑96 (MQ=255)
                                                                            tctcCAGGCCCAGCGCGGC‑TGGCTACGCCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGTTTTATCCGCCAGCTTGGCATTCGCACAcc                 <  1:5561208/96‑1 (MQ=255)
                                                                            accccAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc                 <  1:6048899/93‑1 (MQ=255)
                                                                              tcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCtt               >  2:2570441/1‑96 (MQ=255)
                                                                              tcCAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCtt               <  2:10510860/96‑1 (MQ=255)
                                                                               ccAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc              >  2:12569850/1‑96 (MQ=255)
                                                                               ccAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc              >  1:1057659/1‑96 (MQ=255)
                                                                                cAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCa             <  2:11938121/96‑1 (MQ=255)
                                                                                cAGGCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCa             >  2:12250847/1‑96 (MQ=255)
                                                                                   gCCCAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt          <  1:8248347/96‑1 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTTCCGAAGGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg         >  2:8546904/1‑96 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGGACCCCTTCAACTg         <  1:10970568/96‑1 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg         >  2:5176744/1‑96 (MQ=255)
                                                                                    cccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAAAAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAATg         >  2:13204150/1‑96 (MQ=255)
                                                                                     ccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCTAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa        >  1:9336775/1‑96 (MQ=255)
                                                                                     ccAGCGCGGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa        >  2:2711717/1‑96 (MQ=255)
                                                                                        gcgcGGC‑TGGCTGCGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaaca     <  1:4271137/96‑1 (MQ=255)
                                                                                         cgcgGCTT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCCCACCTTCAACTGaacaa    <  1:1649828/96‑1 (MQ=255)
                                                                                         cgcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaa    >  1:10310608/1‑96 (MQ=255)
                                                                                         cgcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaa    <  2:11115140/96‑1 (MQ=255)
                                                                                          gcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaac   >  2:10005775/1‑96 (MQ=255)
                                                                                          gcgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaacaac   >  2:5580219/1‑96 (MQ=255)
                                                                                           cgGC‑TGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAAcc  <  2:8714842/96‑1 (MQ=255)
                                                                                                 |                                                                                          
TTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC  >  NC_000913/4449642‑4449827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 279 total aligned reads displayed.
GGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTC  >  NC_000913/4449638‑4449838
                                                                                                      |                                                                                                     
GGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑G                                                                                                       >  SRR4302518.3144178/1‑101 (MQ=60)
GGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑G                                                                                                       <  SRR4302518.4392210/101‑1 (MQ=60)
 NNNTTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑G‑G                                                                                                     <  SRR4302518.11379035/101‑1 (MQ=60)
 GCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑G‑G                                                                                                     >  SRR4302518.4118455/1‑101 (MQ=60)
  CATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTGTCCAGGCCCAGCGCGGC‑T‑N‑NN                                                                                                    >  SRR4302518.13379016/1‑101 (MQ=60)
   ATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCGTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑G‑NNN                                                                                                   >  SRR4302518.10620915/1‑101 (MQ=60)
    TTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTA                                                                                                   >  SRR4302518.3026470/1‑101 (MQ=60)
     TGGCGTCTGCCCGGAAGACAGGAAAGCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTAC                                                                                                  <  SRR4302518.1987407/101‑1 (MQ=60)
       GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTTCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGT                                                                                                <  SRR4302518.7053758/101‑1 (MQ=60)
       GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGT                                                                                                >  SRR4302518.2594805/1‑101 (MQ=60)
        NNNCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTC                                                                                               <  SRR4302518.9483457/101‑1 (MQ=60)
        CTTCTGCGCGGAAGACATGAAATCCGATGGCATCATCGCTGCCGCCTCGGTGCGGTAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTC                                                                                               <  SRR4302518.7107075/101‑1 (MQ=60)
        CTTCTGCCCGGAAAACAGTAAAACCGATGGCATCATCGTTGCCGACTAGGTGCGGGAAAATATCATCCTCGTTGTCCAGGTTCAGCGCGGC‑TCG‑ATACGTC                                                                                               >  SRR4302518.127401/1‑101 (MQ=45)
         TTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCC                                                                                              <  SRR4302518.5248404/101‑1 (MQ=60)
         TTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGGGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑Ggctacgnnn                                                                                              >  SRR4302518.10855270/1‑92 (MQ=60)
             NNNCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATT                                                                                          <  SRR4302518.13949782/101‑1 (MQ=60)
               nnngaatacagcacAACCGATTGCAGCATCGCTGTCGCCTCGTTGCGGGAAAATATCGTCCTCGCTCTCCAGGCCCAGCTCGGC‑TGG‑CTACGTCCCATTTC                                                                                        <  SRR4302518.8400887/87‑1 (MQ=57)
               CCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGCCCCGTTTC                                                                                        >  SRR4302518.155154/1‑101 (MQ=60)
               CCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCATCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTGCGTCCCATTTC                                                                                        >  SRR4302518.6564369/1‑101 (MQ=60)
               CCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGGGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGGCCCATTTC                                                                                        >  SRR4302518.4111696/1‑101 (MQ=60)
                 NNNAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCC                                                                                      <  SRR4302518.10478237/101‑1 (MQ=60)
                 NNNAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCC                                                                                      <  SRR4302518.12476399/101‑1 (MQ=60)
                 GGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTNNN                                                                                      >  SRR4302518.8756476/1‑101 (MQ=60)
                 GGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCC                                                                                      <  SRR4302518.3137869/101‑1 (MQ=60)
                 GGAAGACAGGAAAACCGAGGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGTCCCAGCGCGGCGG‑G‑CTACGTCCCATTTCCC                                                                                      >  SRR4302518.6460671/1‑101 (MQ=60)
                  GAGGACAGGCAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCG                                                                                     <  SRR4302518.5646917/101‑1 (MQ=60)
                   NNNACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGC                                                                                    <  SRR4302518.13962073/101‑1 (MQ=60)
                   AAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGC                                                                                    >  SRR4302518.6606677/1‑101 (MQ=60)
                    AGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCA                                                                                   >  SRR4302518.1153097/1‑101 (MQ=60)
                     GACAGGAAAACCGATGGCATCATCGCTGCCGGCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCTA                                                                                  >  SRR4302518.3634609/1‑101 (MQ=60)
                     GACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGCCCCATTTCCCGNNN                                                                                  >  SRR4302518.9010299/1‑101 (MQ=60)
                      ACAGGAACACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAA                                                                                 <  SRR4302518.2162150/101‑1 (MQ=60)
                      AAAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCNNN                                                                                 >  SRR4302518.8039194/1‑101 (MQ=60)
                       CAGGAAAACCGATTGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGCCCCATTTCCCGCANNN                                                                                >  SRR4302518.14536344/1‑101 (MQ=60)
                        NNNAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGA                                                                               <  SRR4302518.14718498/101‑1 (MQ=60)
                        ATGAAAACCGATGCCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCGCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGA                                                                               <  SRR4302518.3416117/101‑1 (MQ=60)
                        AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAANNN                                                                               >  SRR4302518.9138464/1‑101 (MQ=60)
                        AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAANNN                                                                               >  SRR4302518.7362730/1‑101 (MQ=60)
                        AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGA                                                                               >  SRR4302518.3837564/1‑101 (MQ=60)
                          NNNAACCGATGGCATCCTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAAC                                                                             <  SRR4302518.12168469/101‑1 (MQ=60)
                          NNNAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAAC                                                                             <  SRR4302518.14833226/101‑1 (MQ=60)
                          GAAACCCGATGGCATCATCGCTGCCGCCTCGATGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAAC                                                                             <  SRR4302518.1729308/101‑1 (MQ=60)
                          GAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGNNN                                                                             >  SRR4302518.14001111/1‑101 (MQ=60)
                           NNNACCGATGGCATCGTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACA                                                                            <  SRR4302518.7580555/101‑1 (MQ=60)
                           AAAACCGGTGCTATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACA                                                                            <  SRR4302518.5638597/101‑1 (MQ=60)
                           AAAACCGATGGCATCATCGCTGCCGCCTCGGTGGGGGAAAATATCATCCTCGCTATCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTACCGCAAAGANNN                                                                            >  SRR4302518.8549721/1‑101 (MQ=60)
                            AAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAANNN                                                                           >  SRR4302518.11688015/1‑101 (MQ=60)
                             NNNCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGC                                                                          <  SRR4302518.9019602/101‑1 (MQ=60)
                             AACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACNNN                                                                          >  SRR4302518.8234874/1‑101 (MQ=60)
                               CGGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAA                                                                        >  SRR4302518.553412/1‑101 (MQ=60)
                                NNNTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                                       <  SRR4302518.10066106/101‑1 (MQ=60)
                                CGATGGCATCATCTCTGCCGCCTCGGTGCGGGAATATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                                       <  SRR4302518.6757006/101‑1 (MQ=60)
                                CGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGActcgcnnn                                                                       >  SRR4302518.10919975/1‑93 (MQ=60)
                                CGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                                       >  SRR4302518.1824023/1‑101 (MQ=60)
                                 GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGA                                                                      <  SRR4302518.6361637/101‑1 (MQ=60)
                                 GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGA                                                                      >  SRR4302518.3799693/1‑101 (MQ=60)
                                 GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGA                                                                      >  SRR4302518.5539470/1‑101 (MQ=60)
                                  NNNGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAG                                                                     <  SRR4302518.13857746/101‑1 (MQ=60)
                                  NNNGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAG                                                                     <  SRR4302518.14082014/101‑1 (MQ=60)
                                  ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAANNN                                                                     >  SRR4302518.8288787/1‑101 (MQ=60)
                                    GCCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAACAAGANNN                                                                   >  SRR4302518.7947436/1‑101 (MQ=60)
                                     GCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGNNN                                                                  >  SRR4302518.10857562/1‑101 (MQ=60)
                                      nnnccTCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                                 <  SRR4302518.8002894/96‑1 (MQ=60)
                                      CATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCGATTTCCCGCAAAGAACAGCAAGAGANNN                                                                 >  SRR4302518.10708260/1‑101 (MQ=60)
                                      CATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                                 <  SRR4302518.3197288/101‑1 (MQ=60)
                                      CATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                                 <  SRR4302518.3440796/101‑1 (MQ=60)
                                       acatcggctcccgctccgtTGCGGAAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGC                                                                <  SRR4302518.2706740/82‑1 (MQ=60)
                                       ATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATNNN                                                                >  SRR4302518.12070169/1‑101 (MQ=60)
                                       AACATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATNNN                                                                >  SRR4302518.8037595/1‑101 (MQ=60)
                                        TCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCC                                                               >  SRR4302518.5828184/1‑101 (MQ=60)
                                        TCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCC                                                               <  SRR4302518.6879270/101‑1 (MQ=60)
                                         NNNCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCG                                                              <  SRR4302518.11359260/101‑1 (MQ=60)
                                         CATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCATCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAGGAACAGCTAGAGATTtgcg                                                              >  SRR4302518.911118/1‑97 (MQ=60)
                                         CATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAGGAACAGCAAGAGATTGCCG                                                              >  SRR4302518.3220499/1‑101 (MQ=60)
                                         CATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATCGCCG                                                              >  SRR4302518.4723228/1‑101 (MQ=60)
                                           TCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCNNN                                                            >  SRR4302518.12618933/1‑101 (MQ=60)
                                            NNNTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAC                                                           <  SRR4302518.9787356/101‑1 (MQ=60)
                                            NNNTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAC                                                           <  SRR4302518.13783132/101‑1 (MQ=60)
                                            NNNTGCCGCCTCGGTGAGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCTGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAC                                                           <  SRR4302518.12969631/101‑1 (MQ=60)
                                              nnncctCCTCGGTGCGGGAAAATATCATACTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGC                                                         <  SRR4302518.12892376/95‑1 (MQ=60)
                                              CTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGCACGC                                                         >  SRR4302518.3506406/1‑101 (MQ=60)
                                               TGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTTTCCAGGGCGAGCGCGGC‑TGG‑CTACTTCCCATTGCCCGCAAAGAACAGCAAGAAATTGCCGCACGCT                                                        >  SRR4302518.231510/1‑101 (MQ=60)
                                               TGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACNNN                                                        >  SRR4302518.13066182/1‑101 (MQ=60)
                                               TGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTGCCCGCAAAGAACAGCAAGTGATTGCCGAACGCT                                                        >  SRR4302518.1077534/1‑101 (MQ=60)
                                                NNNGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTT                                                       <  SRR4302518.13706096/101‑1 (MQ=60)
                                                GCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTT                                                       <  SRR4302518.4446275/101‑1 (MQ=60)
                                                GCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAAAAGCAAGCGAGTGCCGAACGCTT                                                       >  SRR4302518.4075164/1‑101 (MQ=60)
                                                 NNNCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTT                                                      <  SRR4302518.12730784/101‑1 (MQ=60)
                                                 NNNCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTT                                                      <  SRR4302518.14589556/101‑1 (MQ=60)
                                                 CCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTT                                                      <  SRR4302518.2262112/101‑1 (MQ=60)
                                                 CCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTT                                                      >  SRR4302518.1969679/1‑101 (MQ=60)
                                                  CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCACAGAACAGCAAGAGATTGCCGAACGCTATA                                                     >  SRR4302518.1776747/1‑101 (MQ=60)
                                                  CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTA                                                     <  SRR4302518.5731871/101‑1 (MQ=60)
                                                  CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTA                                                     >  SRR4302518.2831860/1‑101 (MQ=60)
                                                  CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTNNN                                                     >  SRR4302518.14004666/1‑101 (MQ=60)
                                                  CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCAATTCCCGCAAAGAAGAGCAATATATTGTCGAACGCTTTT                                                     >  SRR4302518.2927680/1‑101 (MQ=60)
                                                   GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCCAAGAACAGCAAGAGATTGCCGAACGCTGTAT                                                    >  SRR4302518.4504884/1‑101 (MQ=60)
                                                   GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCATATATCAGCAAGAGATTGCCGgacacttnnn                                                    >  SRR4302518.12956634/1‑91 (MQ=60)
                                                   GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                                    >  SRR4302518.446014/1‑101 (MQ=60)
                                                   GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                                    <  SRR4302518.5289715/101‑1 (MQ=60)
                                                    CCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTNNN                                                   >  SRR4302518.7406020/1‑101 (MQ=60)
                                                    CCTCGGGGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTNNN                                                   >  SRR4302518.8574022/1‑101 (MQ=60)
                                                     CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCGAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                                  <  SRR4302518.3334728/101‑1 (MQ=60)
                                                     CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                                  >  SRR4302518.5262621/1‑101 (MQ=60)
                                                     CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                                  <  SRR4302518.5562515/101‑1 (MQ=60)
                                                     CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTCTATCC                                                  >  SRR4302518.3000183/1‑101 (MQ=60)
                                                     CGCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTANNN                                                  >  SRR4302518.11325159/1‑101 (MQ=60)
                                                      TCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATNNN                                                 >  SRR4302518.7770489/1‑101 (MQ=60)
                                                      NNNGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCAG                                                 <  SRR4302518.9838468/101‑1 (MQ=60)
                                                       CGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCNNN                                                >  SRR4302518.9424084/1‑101 (MQ=60)
                                                       CGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGC                                                >  SRR4302518.1862143/1‑101 (MQ=60)
                                                        GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCNNN                                               >  SRR4302518.12471575/1‑101 (MQ=60)
                                                         NNNCGGGAAAATATCATCCTCGCTCACCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCGA                                              <  SRR4302518.7909915/101‑1 (MQ=60)
                                                         GTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGNNN                                              >  SRR4302518.13611217/1‑101 (MQ=60)
                                                          TGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCNNN                                             >  SRR4302518.7600096/1‑101 (MQ=60)
                                                           NNNGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGC                                            <  SRR4302518.7631683/101‑1 (MQ=60)
                                                           GCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCNNN                                            >  SRR4302518.14257716/1‑101 (MQ=60)
                                                            CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCT                                           >  SRR4302518.1686181/1‑101 (MQ=60)
                                                             GGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                          >  SRR4302518.2602639/1‑101 (MQ=60)
                                                             GGGAAAATATCATCCTCGCTATCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCACTTCCAGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCAAGCTT                                          <  SRR4302518.4422026/101‑1 (MQ=60)
                                                              NNNAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTG                                         <  SRR4302518.10277595/101‑1 (MQ=60)
                                                              GGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTG                                         >  SRR4302518.1460611/1‑101 (MQ=60)
                                                               NNNAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTCG                                        <  SRR4302518.9784721/101‑1 (MQ=60)
                                                               GAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                        >  SRR4302518.4666603/1‑101 (MQ=60)
                                                                 AAATATCAACCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAAAAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGTA                                      <  SRR4302518.3527094/101‑1 (MQ=60)
                                                                  AATCTCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAT                                     <  SRR4302518.6108142/101‑1 (MQ=60)
                                                                   NNNTCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATT                                    <  SRR4302518.15020153/101‑1 (MQ=60)
                                                                   ATATCATCCTCTCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATT                                    <  SRR4302518.2968617/101‑1 (MQ=60)
                                                                   ATATCATCCTCGCTCTCCAGGCCGAGCGCGGC‑TGG‑CGACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCCGCTTGGgnnn                                    >  SRR4302518.13766875/1‑97 (MQ=60)
                                                                   ATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGTCGAACGCTTTATCCGCCAGCTTGGCNNN                                    >  SRR4302518.13372036/1‑101 (MQ=60)
                                                                   ATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATT                                    <  SRR4302518.2352143/101‑1 (MQ=60)
                                                                    TATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                                   <  SRR4302518.6632115/101‑1 (MQ=60)
                                                                      TCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATGCGCCAGCTTGGGATTCGC                                 >  SRR4302518.1393533/1‑101 (MQ=60)
                                                                      TCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCGGCCAGCTTGGCATTNNN                                 >  SRR4302518.9161623/1‑101 (MQ=60)
                                                                      TCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGC                                 <  SRR4302518.5548228/101‑1 (MQ=60)
                                                                      TCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGC                                 >  SRR4302518.1098060/1‑101 (MQ=60)
                                                                      TCATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGC                                 <  SRR4302518.6239945/101‑1 (MQ=60)
                                                                       CATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCA                                <  SRR4302518.6720402/101‑1 (MQ=60)
                                                                        ATCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGGCCCATTTCCCGCAAAGAACAGCAAGAAATTACCGAACGCTTTATCCGCCAGCctgacaatcgcgc                               >  SRR4302518.687253/1‑88 (MQ=60)
                                                                         nnngCGCTCTCCAGGCCCAGCGCGGC‑T‑GCCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACA                              <  SRR4302518.10561048/97‑1 (MQ=60)
                                                                         TCCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCNNN                              >  SRR4302518.10410961/1‑101 (MQ=60)
                                                                         NNNTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACA                              <  SRR4302518.10957311/101‑1 (MQ=60)
                                                                          NNNCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                             <  SRR4302518.12567431/101‑1 (MQ=60)
                                                                          NNNCGCTCTCCAGGCCAAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                             <  SRR4302518.10371029/101‑1 (MQ=60)
                                                                          CCTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                             >  SRR4302518.2327007/1‑101 (MQ=60)
                                                                           CTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATAGCCGAACGCTTTATCCGCCAGCTTGGCATTagtacnnn                            >  SRR4302518.14346138/1‑93 (MQ=60)
                                                                           CTCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGCCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGTTTTATCCGCCAGCTTGGCATTCGCACACC                            <  SRR4302518.5843590/101‑1 (MQ=60)
                                                                           CTCGCACCCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACC                            <  SRR4302518.6355825/101‑1 (MQ=60)
                                                                            TCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACANNN                           >  SRR4302518.14300323/1‑101 (MQ=60)
                                                                            TCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                           >  SRR4302518.6341091/1‑101 (MQ=60)
                                                                            TCGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                           >  SRR4302518.1884823/1‑101 (MQ=60)
                                                                            TCGCTCTCCAGGCACAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                           <  SRR4302518.6571359/101‑1 (MQ=60)
                                                                             CGCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTT                          >  SRR4302518.2974800/1‑101 (MQ=60)
                                                                              NNNCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTC                         <  SRR4302518.7400568/101‑1 (MQ=60)
                                                                              NNNCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTCC                         <  SRR4302518.7757388/101‑1 (MQ=60)
                                                                              GCTCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCNNN                         >  SRR4302518.8679238/1‑101 (MQ=60)
                                                                               NNNTCCAGCCCCAGCGCGCC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                        <  SRR4302518.15021268/101‑1 (MQ=60)
                                                                                TCTCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAA                       >  SRR4302518.6449364/1‑101 (MQ=60)
                                                                                  TCCAGGCCCAGCGGGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCATGATATTGCCGAACGCTTTATCTGCCAGCTTTGTATTCGCACACCTTCANNN                     >  SRR4302518.14478797/1‑101 (MQ=60)
                                                                                  TCCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCANNN                     >  SRR4302518.7968846/1‑101 (MQ=60)
                                                                                   CCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCTCACACCTTCAACTG                    >  SRR4302518.5611398/1‑101 (MQ=60)
                                                                                   CCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG                    <  SRR4302518.4828675/101‑1 (MQ=60)
                                                                                   CCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG                    <  SRR4302518.4866931/101‑1 (MQ=60)
                                                                                   CCAGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG                    >  SRR4302518.1099305/1‑101 (MQ=60)
                                                                                     AGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCCACTNNN                  >  SRR4302518.8735706/1‑101 (MQ=60)
                                                                                     AGGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTCGCATTCGCACACATTCAACTGAA                  >  SRR4302518.697226/1‑101 (MQ=60)
                                                                                     ACGCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAA                  >  SRR4302518.3591806/1‑101 (MQ=60)
                                                                                       GCCCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA                <  SRR4302518.1520584/101‑1 (MQ=60)
                                                                                       GCCCAGCGCGGC‑TGG‑CTACGTCCCATCTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA                <  SRR4302518.2882679/101‑1 (MQ=60)
                                                                                         CCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCTAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACNNN              >  SRR4302518.9787814/1‑101 (MQ=60)
                                                                                         CCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCCACTGAACAAC              >  SRR4302518.1622238/1‑101 (MQ=60)
                                                                                         CCAGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAAC              >  SRR4302518.2019026/1‑101 (MQ=60)
                                                                                          NNNCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC             <  SRR4302518.10168689/101‑1 (MQ=60)
                                                                                           NNNGCGGC‑TGG‑CTACGTCCCATTTCCCGTAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCG            <  SRR4302518.10566377/101‑1 (MQ=60)
                                                                                           AGCGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAANNN            >  SRR4302518.14154557/1‑101 (MQ=60)
                                                                                             gggccgctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCATGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACNNN           >  SRR4302518.10628479/10‑101 (MQ=60)
                                                                                             CGCGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCNNN          >  SRR4302518.10796515/1‑101 (MQ=60)
                                                                                              nnnggctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGAT          <  SRR4302518.10517962/93‑1 (MQ=60)
                                                                                               CGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCCCACCTTCAACTGAACAACCGATTG        <  SRR4302518.2667268/101‑1 (MQ=60)
                                                                                               CGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTG        <  SRR4302518.1529564/101‑1 (MQ=60)
                                                                                               CGGC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGANNN        >  SRR4302518.8665944/1‑101 (MQ=60)
                                                                                                nnnctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTG        <  SRR4302518.7645895/95‑1 (MQ=60)
                                                                                                nnnctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATGG        <  SRR4302518.12010025/95‑1 (MQ=60)
                                                                                                 nnntgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGA       <  SRR4302518.7705383/96‑1 (MQ=60)
                                                                                                 gtctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGA       <  SRR4302518.3493316/96‑1 (MQ=60)
                                                                                                 NN‑N‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGATT     <  SRR4302518.9861124/101‑1 (MQ=60)
                                                                                                 NN‑N‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAAT     <  SRR4302518.7945619/101‑1 (MQ=60)
                                                                                                 NN‑N‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAAT     <  SRR4302518.15042644/101‑1 (MQ=60)
                                                                                                 NN‑N‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAAT     <  SRR4302518.10930967/101‑1 (MQ=60)
                                                                                                 GC‑TGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCATGGCATTCGCACACCTTCAACTGAACAACCGATTNNN      >  SRR4302518.10079923/1‑101 (MQ=60)
                                                                                                   ctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGNNN     >  SRR4302518.8940046/4‑101 (MQ=60)
                                                                                                   ctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAAT     >  SRR4302518.6808464/4‑101 (MQ=60)
                                                                                                   ctgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAAT     >  SRR4302518.2743958/4‑101 (MQ=60)
                                                                                                    tgG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGTTTGAATT    >  SRR4302518.6807072/3‑101 (MQ=60)
                                                                                                    N‑N‑NTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTT   <  SRR4302518.8211714/101‑1 (MQ=60)
                                                                                                    G‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGGGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAAGAACCGATTGAANNN   >  SRR4302518.12523479/1‑101 (MQ=60)
                                                                                                    G‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACACCCGATTGAANNN   >  SRR4302518.14835027/1‑101 (MQ=60)
                                                                                                    G‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTT   >  SRR4302518.4380209/1‑101 (MQ=60)
                                                                                                    G‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTT   >  SRR4302518.3626496/1‑101 (MQ=60)
                                                                                                    G‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAANNN   >  SRR4302518.10441913/1‑101 (MQ=60)
                                                                                                      |                                                                                                     
GGCATTGGCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGC‑T‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTC  >  NC_000913/4449638‑4449838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑