Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 791,797 T→C G405G (GGA→GGG)  modF ← ModF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913791,7970TC99.5% 733.5 / ‑4.7 211G405G (GGA→GGG) modFModF
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (104/106);  total (105/106)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

Only 100 of 220 total aligned reads displayed.

CAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCG  >  NC_000913/791707‑791886
                                                                                          |                                                                                         
 aGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGcccc                                                                                        >  2:2979642/1‑93 (MQ=255)
   cGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATTTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCa                                                                                      <  2:5892800/93‑1 (MQ=255)
    ggATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAg                                                                                     <  1:636778/93‑1 (MQ=255)
    ggATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAg                                                                                     >  2:6469099/1‑93 (MQ=255)
     gATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAgg                                                                                    >  2:1445260/1‑93 (MQ=255)
     gATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAgg                                                                                    >  2:3011608/1‑93 (MQ=255)
      aTCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGa                                                                                   >  2:4532610/1‑93 (MQ=255)
         aaGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAg                                                                                >  2:6132493/1‑93 (MQ=255)
         aaGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAg                                                                                >  2:472731/1‑93 (MQ=255)
          aGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGa                                                                               >  1:1014744/1‑93 (MQ=255)
           gCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGTGCCAGACGCTGCTGCCCCCAGGAAAGAc                                                                              >  2:6213153/1‑93 (MQ=255)
           gCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGAc                                                                              <  2:579065/93‑1 (MQ=255)
           gCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGAc                                                                              <  2:512854/93‑1 (MQ=255)
             cccTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTa                                                                            >  2:11599/1‑93 (MQ=255)
                tGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATgg                                                                         <  1:1735826/93‑1 (MQ=255)
                  tAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGaa                                                                       >  2:3861298/1‑93 (MQ=255)
                  tAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGaa                                                                       <  1:2687685/93‑1 (MQ=255)
                   aGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAAc                                                                      <  2:1818946/93‑1 (MQ=255)
                   aGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAAc                                                                      >  1:2389884/1‑93 (MQ=255)
                   aGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAAc                                                                      <  2:5068936/93‑1 (MQ=255)
                     tGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACgg                                                                    <  1:4113273/93‑1 (MQ=255)
                       gTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAg                                                                  >  2:1937487/1‑93 (MQ=255)
                         tCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCg                                                                <  1:1637738/93‑1 (MQ=255)
                         tCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCg                                                                >  1:5562514/1‑93 (MQ=255)
                              gagaATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGc                                                           >  2:2523461/1‑93 (MQ=255)
                               agaATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGcc                                                          <  1:1490849/93‑1 (MQ=255)
                               agaATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGcc                                                          <  2:3836955/93‑1 (MQ=255)
                                  aTAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTg                                                       >  2:2388669/1‑93 (MQ=255)
                                   tAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGc                                                      >  1:1659431/1‑93 (MQ=255)
                                    aaGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCg                                                     <  2:4953650/93‑1 (MQ=255)
                                     aGCAACGTCGGATGTTTCATCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGt                                                    <  1:5766413/93‑1 (MQ=255)
                                     aGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGt                                                    <  2:3603934/93‑1 (MQ=255)
                                     aGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGt                                                    <  2:2130227/93‑1 (MQ=255)
                                     aGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGt                                                    <  1:4663986/93‑1 (MQ=255)
                                      gCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGtt                                                   <  1:5742998/93‑1 (MQ=255)
                                      gCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGtt                                                   >  1:3050946/1‑93 (MQ=255)
                                      gCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGtt                                                   <  1:6189630/93‑1 (MQ=255)
                                       cAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGttt                                                  <  1:3542549/93‑1 (MQ=255)
                                       cAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGttt                                                  <  1:5714806/93‑1 (MQ=255)
                                        aaCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTa                                                 >  1:4742197/1‑93 (MQ=255)
                                        aaCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTa                                                 <  2:4793683/93‑1 (MQ=255)
                                         aCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAt                                                >  2:2588171/1‑93 (MQ=255)
                                         aCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAt                                                >  1:2194565/1‑93 (MQ=255)
                                          cGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATc                                               >  2:549199/1‑93 (MQ=255)
                                            tCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCaa                                             <  2:3190096/93‑1 (MQ=255)
                                             cGGATGTTTCACCAGTGCGCGGACAATCAGCGGCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCCAt                                            >  1:4949645/1‑93 (MQ=255)
                                              ggATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATg                                           >  2:749055/1‑93 (MQ=255)
                                              ggATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATg                                           >  1:5966630/1‑93 (MQ=255)
                                               gATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGc                                          >  1:945995/1‑93 (MQ=255)
                                               gATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGc                                          <  2:405184/93‑1 (MQ=255)
                                               gATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGc                                          >  1:5067141/1‑93 (MQ=255)
                                               gATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGc                                          >  2:5319530/1‑93 (MQ=255)
                                                aTGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGcc                                         <  1:2097776/93‑1 (MQ=255)
                                                   tttCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCgag                                      <  1:5896448/93‑1 (MQ=255)
                                                    ttCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCgaga                                     >  1:405667/1‑93 (MQ=255)
                                                      cACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAat                                   <  2:3676948/93‑1 (MQ=255)
                                                        ccAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAatat                                 >  1:6115609/1‑93 (MQ=255)
                                                        ccAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAatat                                 >  2:207554/1‑93 (MQ=255)
                                                        ccAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAatat                                 >  2:3151287/1‑93 (MQ=255)
                                                         cAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATc                                >  1:3409406/1‑93 (MQ=255)
                                                          aGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATcc                               <  2:2647203/93‑1 (MQ=255)
                                                          aGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATcc                               >  2:5609617/1‑93 (MQ=255)
                                                          aGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATcc                               <  2:5818241/93‑1 (MQ=255)
                                                          aGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATcc                               <  2:3318524/93‑1 (MQ=255)
                                                           gTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCa                              >  2:4237370/1‑93 (MQ=255)
                                                           gTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCa                              >  1:6286168/1‑93 (MQ=255)
                                                            tGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAg                             <  2:1557320/93‑1 (MQ=255)
                                                                cgGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAc                         <  2:2783689/93‑1 (MQ=255)
                                                                cgGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAc                         <  2:4894395/93‑1 (MQ=255)
                                                                cgGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAc                         >  2:4447161/1‑93 (MQ=255)
                                                                 ggACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAct                        <  2:1317237/93‑1 (MQ=255)
                                                                 ggACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAct                        >  2:5563826/1‑93 (MQ=255)
                                                                  gACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActg                       >  1:3803488/1‑93 (MQ=255)
                                                                  gACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActg                       >  1:4775567/1‑93 (MQ=255)
                                                                  gACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGGCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActg                       >  2:3584324/1‑93 (MQ=255)
                                                                  gACAATAAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActg                       >  1:5363396/1‑93 (MQ=255)
                                                                   aCAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGACActgc                      >  2:1924995/1‑93 (MQ=255)
                                                                    cAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgct                     <  2:4427710/93‑1 (MQ=255)
                                                                     aaTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgctg                    <  2:878947/93‑1 (MQ=255)
                                                                     aaTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgctg                    <  1:682672/93‑1 (MQ=255)
                                                                      aTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACtgctgc                   >  2:3517640/1‑93 (MQ=255)
                                                                          gcgcCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCa               >  2:398867/1‑93 (MQ=255)
                                                                          gcgcCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCa               >  1:3593430/1‑93 (MQ=255)
                                                                          gcgcCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCa               >  2:2799442/1‑93 (MQ=255)
                                                                          gccccAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCa               <  1:1761540/90‑1 (MQ=255)
                                                                           cgcCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAg              <  1:3994861/93‑1 (MQ=255)
                                                                             ccAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATACAGCCACTGCTGCACCAGtt            >  1:2628533/1‑93 (MQ=255)
                                                                              cAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttt           >  2:917048/1‑93 (MQ=255)
                                                                               aGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGtttt          <  1:5687747/93‑1 (MQ=255)
                                                                                gACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttttt         >  2:1708124/1‑93 (MQ=255)
                                                                                gACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttttt         <  1:4404656/93‑1 (MQ=255)
                                                                                gACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttttt         >  1:2113678/1‑93 (MQ=255)
                                                                                  cGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGc       <  2:4152776/93‑1 (MQ=255)
                                                                                  cGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGc       <  1:3096011/93‑1 (MQ=255)
                                                                                  cGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGc       <  1:2397937/93‑1 (MQ=255)
                                                                                   gctgctGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCt      >  1:2317814/1‑93 (MQ=255)
                                                                                   gctgctGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCt      <  2:831719/93‑1 (MQ=255)
                                                                                    ctgctgCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTg     >  1:3852687/1‑93 (MQ=255)
                                                                                      gctgCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGc   >  2:2229956/1‑93 (MQ=255)
                                                                                       ctgCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCg  <  1:1302938/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCG  >  NC_000913/791707‑791886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCC  >  NC_000913/791704‑791890
                                                                                             |                                                                                             
ATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGC                                                                                               <  SRR4302534.1635203/94‑1 (MQ=60)
ATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTCCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGC                                                                                               <  SRR4302534.5913091/94‑1 (MQ=60)
  TCAGCGGATCAAGCACCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGAGCGCGGACAATCAGCGCCAGACGCTGCTGCCC                                                                                             >  SRR4302534.6648803/1‑94 (MQ=60)
     GCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGGCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCA                                                                                          <  SRR4302534.206563/94‑1 (MQ=60)
       GGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCCGG                                                                                        <  SRR4302534.4164320/94‑1 (MQ=60)
       GGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGG                                                                                        >  SRR4302534.2932568/1‑94 (MQ=60)
       GGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGG                                                                                        <  SRR4302534.652239/94‑1 (MQ=60)
            AAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGA                                                                                   >  SRR4302534.1040859/1‑94 (MQ=60)
                CCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAT                                                                               >  SRR4302534.4090571/1‑94 (MQ=60)
                   TGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGA                                                                            <  SRR4302534.2377794/94‑1 (MQ=60)
                   TGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGA                                                                            <  SRR4302534.587115/94‑1 (MQ=60)
                   TGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGA                                                                            <  SRR4302534.1780904/94‑1 (MQ=60)
                     TAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAAC                                                                          >  SRR4302534.2453387/1‑94 (MQ=60)
                     TAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAAC                                                                          >  SRR4302534.2458293/1‑94 (MQ=60)
                     TAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAAC                                                                          <  SRR4302534.2758713/94‑1 (MQ=60)
                      AGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACG                                                                         >  SRR4302534.387741/1‑94 (MQ=60)
                      AGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACG                                                                         <  SRR4302534.6244248/94‑1 (MQ=60)
                       GTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGG                                                                        >  SRR4302534.4565555/1‑94 (MQ=60)
                        TGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGA                                                                       <  SRR4302534.4223925/94‑1 (MQ=60)
                        TGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGA                                                                       >  SRR4302534.5133353/1‑94 (MQ=60)
                         GGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAG                                                                      <  SRR4302534.3654338/94‑1 (MQ=60)
                         GGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAG                                                                      <  SRR4302534.6426106/94‑1 (MQ=60)
                         GGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGAACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAG                                                                      <  SRR4302534.4884046/94‑1 (MQ=60)
                           TTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCG                                                                    >  SRR4302534.5712700/1‑94 (MQ=60)
                           TTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCG                                                                    <  SRR4302534.5812759/94‑1 (MQ=60)
                            TCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGT                                                                   >  SRR4302534.4217551/1‑94 (MQ=60)
                            TCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGT                                                                   >  SRR4302534.551430/1‑94 (MQ=60)
                            TCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGT                                                                   <  SRR4302534.1680450/94‑1 (MQ=60)
                               TCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAG                                                                <  SRR4302534.6065941/94‑1 (MQ=60)
                                  AGAATAAGCACCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCAAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCG                                                             <  SRR4302534.2464644/94‑1 (MQ=60)
                                  AGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCG                                                             <  SRR4302534.1530065/94‑1 (MQ=60)
                                   GAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGT                                                            <  SRR4302534.5845015/94‑1 (MQ=60)
                                    AATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTG                                                           <  SRR4302534.1374675/94‑1 (MQ=60)
                                     ATAAGCAACGTCGGATGTTTCACCAGTGCTCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGC                                                          >  SRR4302534.4444803/1‑94 (MQ=60)
                                     ATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGC                                                          >  SRR4302534.1702656/1‑94 (MQ=60)
                                      TAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCG                                                         <  SRR4302534.3950859/94‑1 (MQ=60)
                                       AAGCAACGTCGGATGTTTCACCAGGGCGCGGACAATCAGGGCCAGACGCTGCTGCCCCCAGGCAAGACTATGGAACGGAGGGTCAGCCGCGCGT                                                        >  SRR4302534.4125961/1‑94 (MQ=60)
                                        AGCAACGTCGGATGTTTCATCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTT                                                       <  SRR4302534.5922853/94‑1 (MQ=60)
                                        AGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTT                                                       >  SRR4302534.3130990/1‑94 (MQ=60)
                                        AGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTT                                                       <  SRR4302534.4790613/94‑1 (MQ=60)
                                         GCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTT                                                      <  SRR4302534.5898689/94‑1 (MQ=60)
                                         GCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTT                                                      <  SRR4302534.6358073/94‑1 (MQ=60)
                                          CAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTA                                                     <  SRR4302534.5869636/94‑1 (MQ=60)
                                          CAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTA                                                     <  SRR4302534.3637843/94‑1 (MQ=60)
                                          CAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTA                                                     >  SRR4302534.4870927/1‑94 (MQ=60)
                                           AACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAT                                                    >  SRR4302534.2252618/1‑94 (MQ=60)
                                              GTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCTCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAA                                                 >  SRR4302534.6473711/1‑94 (MQ=60)
                                              GTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAA                                                 <  SRR4302534.6001051/94‑1 (MQ=60)
                                               TCGGATGTTTCACCAGTGCGCGGACAATCAGCGGCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCCAT                                                >  SRR4302534.5083708/1‑94 (MQ=60)
                                               TCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAAT                                                >  SRR4302534.1683999/1‑94 (MQ=60)
                                                CGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATG                                               >  SRR4302534.6128658/1‑94 (MQ=60)
                                                CGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATG                                               <  SRR4302534.2187729/94‑1 (MQ=60)
                                                 GGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGC                                              >  SRR4302534.5203944/1‑94 (MQ=60)
                                                 GGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGC                                              <  SRR4302534.5909366/94‑1 (MQ=60)
                                                 GGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGC                                              >  SRR4302534.970064/1‑94 (MQ=60)
                                                  GATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCC                                             <  SRR4302534.5228115/94‑1 (MQ=60)
                                                  GATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCC                                             <  SRR4302534.2147498/94‑1 (MQ=60)
                                                   ATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCG                                            <  SRR4302534.2152816/94‑1 (MQ=60)
                                                    TGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGA                                           <  SRR4302534.4268193/94‑1 (MQ=60)
                                                     GTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAG                                          <  SRR4302534.1290869/94‑1 (MQ=60)
                                                     GTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAG                                          <  SRR4302534.2269118/94‑1 (MQ=60)
                                                     GTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAG                                          <  SRR4302534.1764597/94‑1 (MQ=60)
                                                      TTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGA                                         <  SRR4302534.6056551/94‑1 (MQ=60)
                                                      TTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGA                                         >  SRR4302534.416036/1‑94 (MQ=60)
                                                       TTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAA                                        <  SRR4302534.3171834/94‑1 (MQ=60)
                                                        TCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAAT                                       <  SRR4302534.2159649/94‑1 (MQ=60)
                                                         CACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATA                                      >  SRR4302534.3593441/1‑94 (MQ=60)
                                                          ACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATAT                                     >  SRR4302534.915791/1‑94 (MQ=60)
                                                          ACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATAT                                     >  SRR4302534.6281977/1‑94 (MQ=60)
                                                           CCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATC                                    >  SRR4302534.3500977/1‑94 (MQ=60)
                                                             AGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCA                                  >  SRR4302534.6456249/1‑94 (MQ=60)
                                                               GGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGC                                <  SRR4302534.4838596/94‑1 (MQ=60)
                                                                GCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCC                               <  SRR4302534.3455043/94‑1 (MQ=60)
                                                                 CGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGTCA                              >  SRR4302534.4980965/1‑94 (MQ=60)
                                                                 CGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCA                              <  SRR4302534.1799659/94‑1 (MQ=60)
                                                                  GCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAC                             >  SRR4302534.2700023/1‑94 (MQ=60)
                                                                   CGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAGGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACT                            <  SRR4302534.1704275/94‑1 (MQ=60)
                                                                    GGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           >  SRR4302534.4905251/1‑94 (MQ=60)
                                                                    GGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           >  SRR4302534.3905618/1‑94 (MQ=60)
                                                                    GGACAATAAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           >  SRR4302534.5507934/1‑94 (MQ=60)
                                                                     GACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGC                          <  SRR4302534.3877598/94‑1 (MQ=60)
                                                                       CAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTG                        <  SRR4302534.6288817/94‑1 (MQ=60)
                                                                        AATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGC                       <  SRR4302534.699341/94‑1 (MQ=60)
                                                                            AGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCA                   >  SRR4302534.3690079/1‑94 (MQ=60)
                                                                             GCCCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAG                  <  SRR4302534.1807235/94‑1 (MQ=60)
                                                                              CGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGT                 <  SRR4302534.4101803/94‑1 (MQ=60)
                                                                               GCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGATTATCCAGCCACTGCTGCACCAGTT                >  SRR4302534.507738/1‑94 (MQ=60)
                                                                               GCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATACAGCCACTGCTGCACCAGTT                >  SRR4302534.2698064/1‑94 (MQ=60)
                                                                                CCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTT               <  SRR4302534.6341623/94‑1 (MQ=60)
                                                                                 CAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTT              >  SRR4302534.2424318/1‑94 (MQ=60)
                                                                                  AGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTT             >  SRR4302534.2169273/1‑94 (MQ=60)
                                                                                  AGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTT             <  SRR4302534.5841771/94‑1 (MQ=60)
                                                                                   GACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTG            <  SRR4302534.4523514/94‑1 (MQ=60)
                                                                                     CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          <  SRR4302534.2461654/94‑1 (MQ=60)
                                                                                     CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          >  SRR4302534.2379322/1‑94 (MQ=60)
                                                                                     CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          <  SRR4302534.3177456/94‑1 (MQ=60)
                                                                                      GCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTG         <  SRR4302534.1577333/94‑1 (MQ=60)
                                                                                      GCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTG         >  SRR4302534.3956124/1‑94 (MQ=60)
                                                                                       CTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGG        >  SRR4302534.5897304/1‑94 (MQ=60)
                                                                                        TGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGC       <  SRR4302534.3041342/94‑1 (MQ=60)
                                                                                          CTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGA     >  SRR4302534.134738/1‑94 (MQ=60)
                                                                                          CTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGA     <  SRR4302534.1337306/94‑1 (MQ=60)
                                                                                          CTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGA     >  SRR4302534.2856173/1‑94 (MQ=60)
                                                                                            GCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATC   <  SRR4302534.3800100/94‑1 (MQ=60)
                                                                                            GCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATC   <  SRR4302534.3830005/94‑1 (MQ=60)
                                                                                             CCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCC  >  SRR4302534.4066803/1‑94 (MQ=60)
                                                                                             CCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCC  >  SRR4302534.4332325/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCC  >  NC_000913/791704‑791890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑