Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 791,797 T→C G405G (GGA→GGG)  modF ← ModF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913791,7970TC99.6% 857.9 / NA 250G405G (GGA→GGG) modFModF
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (138/111);  total (139/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 269 total aligned reads displayed.

CAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGC  >  NC_000913/791707‑791885
                                                                                          |                                                                                        
  gCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCcccc                                                                                      <  1:4830929/93‑1 (MQ=255)
  gCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCcccc                                                                                      <  1:5368727/93‑1 (MQ=255)
  gCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCcccc                                                                                      >  2:8457539/1‑93 (MQ=255)
    gtagcAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAg                                                                                    <  2:8086323/89‑1 (MQ=255)
    ggATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGCCAATCAGCGCCAGACGCTGCTGCCCCCAg                                                                                    >  2:7938550/1‑93 (MQ=255)
    ggATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAg                                                                                    >  1:4615403/1‑93 (MQ=255)
    ggATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAg                                                                                    <  1:6609728/93‑1 (MQ=255)
      aTCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGa                                                                                  <  2:6247428/93‑1 (MQ=255)
       tCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGaa                                                                                 <  2:5492058/93‑1 (MQ=255)
         aaGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAg                                                                               >  1:6714703/1‑93 (MQ=255)
           gCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGAc                                                                             >  2:5860391/1‑93 (MQ=255)
           gCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGAc                                                                             >  1:8187219/1‑93 (MQ=255)
              ccTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAt                                                                          >  2:8666106/1‑93 (MQ=255)
              ccTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAt                                                                          >  2:6445734/1‑93 (MQ=255)
               cTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATg                                                                         >  1:4949996/1‑93 (MQ=255)
               cTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATg                                                                         <  2:1657864/93‑1 (MQ=255)
                tGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATgg                                                                        >  2:3484364/1‑93 (MQ=255)
                tGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATgg                                                                        >  1:8659209/1‑93 (MQ=255)
                 gTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCCGACGCTGCTGTCCCCCGGGAAGAATATGGa                                                                       >  2:5129591/1‑93 (MQ=255)
                 gTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGa                                                                       >  2:153847/1‑93 (MQ=255)
                  tAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGaa                                                                      <  2:3941236/93‑1 (MQ=255)
                  tAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGaa                                                                      <  2:5040055/93‑1 (MQ=255)
                  tAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGaa                                                                      >  1:3682366/1‑93 (MQ=255)
                    gTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGCCTATGGAACg                                                                    >  2:7721145/1‑93 (MQ=255)
                    gTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACg                                                                    >  2:1256576/1‑93 (MQ=255)
                    gTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACg                                                                    >  1:4026607/1‑93 (MQ=255)
                     tGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCGGCCCCCAGGAAAGACTATGGAACgg                                                                   <  1:3038465/93‑1 (MQ=255)
                     aggTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACgg                                                                   <  1:6705153/92‑1 (MQ=255)
                      ggTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGa                                                                  >  2:566055/1‑93 (MQ=255)
                      ggTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGa                                                                  <  1:2621497/93‑1 (MQ=255)
                       gcccATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAg                                                                 >  1:666104/4‑93 (MQ=255)
                       gTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAg                                                                 >  2:8822338/1‑93 (MQ=255)
                        ttCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGc                                                                >  2:8707875/1‑93 (MQ=255)
                              gagaATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGc                                                          >  2:25973/1‑93 (MQ=255)
                                gaATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCg                                                        >  2:1800424/1‑93 (MQ=255)
                                 aaTAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGt                                                       <  1:5127985/93‑1 (MQ=255)
                                 aaTAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGt                                                       >  2:526090/1‑93 (MQ=255)
                                 aaTAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGt                                                       >  1:2104860/1‑93 (MQ=255)
                                    aaGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCg                                                    <  1:4325141/93‑1 (MQ=255)
                                    aaGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCg                                                    >  1:6173871/1‑93 (MQ=255)
                                    aaGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCg                                                    <  2:8146903/93‑1 (MQ=255)
                                     aGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGt                                                   >  1:7364166/1‑93 (MQ=255)
                                     aGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGt                                                   >  2:439595/1‑93 (MQ=255)
                                     aGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGt                                                   >  2:3239987/1‑93 (MQ=255)
                                      gCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCCGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGtt                                                  >  2:2363855/1‑93 (MQ=255)
                                        agcGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTa                                                <  1:3873800/91‑1 (MQ=255)
                                        aaCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTa                                                <  2:1216734/93‑1 (MQ=255)
                                         aCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAt                                               >  1:2699643/1‑93 (MQ=255)
                                         aCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAt                                               >  2:3119608/1‑93 (MQ=255)
                                         aCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAt                                               >  1:291965/1‑93 (MQ=255)
                                           gTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCa                                             >  1:1595311/1‑93 (MQ=255)
                                            tCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCaa                                            <  1:1418329/93‑1 (MQ=255)
                                             cGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAAt                                           >  2:661674/1‑93 (MQ=255)
                                               gATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGc                                         <  2:3279584/93‑1 (MQ=255)
                                                    ttCACCAGTGCGCGGACAATCAGCGACAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGGCgaga                                    <  1:2226235/93‑1 (MQ=255)
                                                     tCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGaa                                   <  2:4792958/93‑1 (MQ=255)
                                                      cACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAat                                  >  2:4847051/1‑93 (MQ=255)
                                                      cACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAat                                  >  2:6151079/1‑93 (MQ=255)
                                                       aCCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAata                                 >  1:5622231/1‑93 (MQ=255)
                                                       aCCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAata                                 <  1:4753678/93‑1 (MQ=255)
                                                        ccAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAatat                                >  1:8035660/1‑93 (MQ=255)
                                                        ccAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAatat                                >  1:858256/1‑93 (MQ=255)
                                                         cAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATc                               <  2:5609958/93‑1 (MQ=255)
                                                          aGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATcc                              <  2:8345584/93‑1 (MQ=255)
                                                           gTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCa                             >  2:4021620/1‑93 (MQ=255)
                                                           gTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCa                             >  2:7388625/1‑93 (MQ=255)
                                                              cgcgGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGcc                          <  2:8636092/93‑1 (MQ=255)
                                                              cgaagACATTCAGCGCCAGACGCTGCGGCCCCTAGGAAAGACTATGGAACGGAACGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGcc                          <  2:3381647/89‑1 (MQ=255)
                                                               gcgGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCa                         >  2:3995239/1‑93 (MQ=255)
                                                                cgGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAc                        <  1:7960527/93‑1 (MQ=255)
                                                                cgGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAc                        >  1:8443418/1‑93 (MQ=255)
                                                                 ggACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAct                       <  1:5542111/93‑1 (MQ=255)
                                                                  gACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActg                      >  2:7008113/1‑93 (MQ=255)
                                                                  gACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActg                      >  1:4087037/1‑93 (MQ=255)
                                                                  gACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActg                      >  2:1338603/1‑93 (MQ=255)
                                                                   gcAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgc                     <  1:2814042/92‑1 (MQ=255)
                                                                     aaTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgctg                   >  1:1124369/1‑93 (MQ=255)
                                                                     aaTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgctg                   >  2:5903092/1‑93 (MQ=255)
                                                                     aaTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgctg                   >  2:3591055/1‑93 (MQ=255)
                                                                      aTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGGGTTTATCAATGCCGAGAATATCCAGCCACtgctgc                  >  1:2950078/1‑93 (MQ=255)
                                                                      aTCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACtgctgc                  >  1:6748815/1‑93 (MQ=255)
                                                                       tCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCa                 <  2:7642246/93‑1 (MQ=255)
                                                                       tCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGGGCGGTTATCAATGCCGGGAATATCCAGCCACTGCTGCa                 >  2:6598307/1‑93 (MQ=255)
                                                                         aGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCAcc               <  2:537955/93‑1 (MQ=255)
                                                                             ccAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGtt           <  2:6194875/93‑1 (MQ=255)
                                                                             ccAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGtt           >  1:966831/1‑93 (MQ=255)
                                                                               aGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGtttt         >  1:1929638/1‑93 (MQ=255)
                                                                               aGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGtttt         >  1:3230477/1‑93 (MQ=255)
                                                                               aGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGtttt         >  1:7741543/1‑93 (MQ=255)
                                                                                gACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttttt        <  2:8237244/93‑1 (MQ=255)
                                                                                gACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttttt        >  2:3024074/1‑93 (MQ=255)
                                                                                gACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttttt        >  1:5412465/1‑93 (MQ=255)
                                                                                 aCGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTg       <  2:797290/93‑1 (MQ=255)
                                                                                  cGCTGCTGCCCCCAGGAAAGACTATGGAGCGGAGCGTCAGCCTTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGc      <  1:5818818/93‑1 (MQ=255)
                                                                                  cGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGc      >  1:8548885/1‑93 (MQ=255)
                                                                                   gctgctGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCt     <  1:4504772/93‑1 (MQ=255)
                                                                                   gctgctGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCt     >  1:6027481/1‑93 (MQ=255)
                                                                                    ctgctgCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCCCCAGTTTTTGCTg    <  2:6483478/93‑1 (MQ=255)
                                                                                    ctgctgCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTg    <  1:6049613/93‑1 (MQ=255)
                                                                                      gctgCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGc  <  1:6978675/93‑1 (MQ=255)
                                                                                          |                                                                                        
CAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGC  >  NC_000913/791707‑791885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCC  >  NC_000913/791709‑791890
                                                                                        |                                                                                             
GCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCA                                                                                          <  SRR4302541.5423843/94‑1 (MQ=60)
GCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCA                                                                                          >  SRR4302541.2166637/1‑94 (MQ=60)
GCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCA                                                                                          <  SRR4302541.4880177/94‑1 (MQ=60)
 CGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAG                                                                                         >  SRR4302541.4662295/1‑94 (MQ=60)
  GGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGG                                                                                        <  SRR4302541.2326974/94‑1 (MQ=60)
  GGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGG                                                                                        <  SRR4302541.6677464/94‑1 (MQ=60)
   GATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGA                                                                                       <  SRR4302541.5438324/94‑1 (MQ=60)
      CAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAG                                                                                    >  SRR4302541.2653720/1‑94 (MQ=60)
      CAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAG                                                                                    >  SRR4302541.6786338/1‑94 (MQ=60)
      CAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAG                                                                                    >  SRR4302541.3237398/1‑94 (MQ=60)
       AAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGGGCCAAACGCTGCTGCCCCCAGGAAAGA                                                                                   >  SRR4302541.2956398/1‑94 (MQ=60)
       AAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGA                                                                                   >  SRR4302541.4925909/1‑94 (MQ=60)
       AAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGA                                                                                   >  SRR4302541.2797939/1‑94 (MQ=60)
        AGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGAC                                                                                  <  SRR4302541.2870907/94‑1 (MQ=60)
        AGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGAC                                                                                  >  SRR4302541.8276590/1‑94 (MQ=60)
          CCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTA                                                                                >  SRR4302541.2940897/1‑94 (MQ=60)
           CCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAT                                                                               <  SRR4302541.5748027/94‑1 (MQ=60)
           CCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAT                                                                               <  SRR4302541.5402288/94‑1 (MQ=60)
           CCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAG                                                                               >  SRR4302541.6780474/1‑94 (MQ=60)
           CCCTGTAGTGGTTAATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTAT                                                                               <  SRR4302541.4983067/94‑1 (MQ=60)
            CCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATG                                                                              <  SRR4302541.7192533/94‑1 (MQ=60)
            CCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATG                                                                              >  SRR4302541.5000588/1‑94 (MQ=60)
             CTGTAGTGGTTCATCGAGAATAAGCAACGTCGTATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGG                                                                             >  SRR4302541.3727466/1‑94 (MQ=60)
             CTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGG                                                                             >  SRR4302541.8751896/1‑94 (MQ=60)
             CTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGG                                                                             >  SRR4302541.3981517/1‑94 (MQ=60)
             CTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGG                                                                             <  SRR4302541.456024/94‑1 (MQ=60)
              TGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGA                                                                            >  SRR4302541.8046437/1‑94 (MQ=60)
               GTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAA                                                                           >  SRR4302541.3719656/1‑94 (MQ=60)
                 AGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCCGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACG                                                                         >  SRR4302541.6748816/1‑94 (MQ=60)
                 AGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACG                                                                         >  SRR4302541.5676970/1‑94 (MQ=60)
                 AGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACG                                                                         >  SRR4302541.4066389/1‑94 (MQ=60)
                  GTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGG                                                                        >  SRR4302541.4117738/1‑94 (MQ=60)
                   TGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGA                                                                       <  SRR4302541.4352667/94‑1 (MQ=60)
                   TGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCGGCCCCCAGGAAAGACTATGGAACGGA                                                                       <  SRR4302541.3069157/94‑1 (MQ=60)
                   AGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGA                                                                       <  SRR4302541.6775742/94‑1 (MQ=60)
                    ggccCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAG                                                                      >  SRR4302541.672383/5‑94 (MQ=60)
                    GGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAG                                                                      <  SRR4302541.2647662/94‑1 (MQ=60)
                     GTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGC                                                                     <  SRR4302541.8611239/94‑1 (MQ=60)
                      TTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCG                                                                    >  SRR4302541.7393287/1‑94 (MQ=60)
                         ATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCA                                                                 >  SRR4302541.5503230/1‑94 (MQ=60)
                         ATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCA                                                                 <  SRR4302541.1572518/94‑1 (MQ=60)
                           CGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGC                                                               >  SRR4302541.8273497/1‑94 (MQ=60)
                            GAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCC                                                              <  SRR4302541.8063888/94‑1 (MQ=60)
                              GAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGT                                                            >  SRR4302541.2125263/1‑94 (MQ=60)
                              GAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGT                                                            >  SRR4302541.8478932/1‑94 (MQ=60)
                               AATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTG                                                           <  SRR4302541.5180700/94‑1 (MQ=60)
                               AATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTG                                                           <  SRR4302541.8491783/94‑1 (MQ=60)
                                ATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGC                                                          <  SRR4302541.4444103/94‑1 (MQ=60)
                                 TAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCG                                                         >  SRR4302541.6236949/1‑94 (MQ=60)
                                  AAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGT                                                        >  SRR4302541.3418691/1‑94 (MQ=60)
                                  AAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGT                                                        <  SRR4302541.4368326/94‑1 (MQ=60)
                                  AAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGT                                                        >  SRR4302541.7442876/1‑94 (MQ=60)
                                   AGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTT                                                       <  SRR4302541.8736750/94‑1 (MQ=60)
                                   AGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTT                                                       >  SRR4302541.5189631/1‑94 (MQ=60)
                                   AGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTT                                                       >  SRR4302541.2828908/1‑94 (MQ=60)
                                    GCAACGTCGGATGTTTCCCCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTT                                                      <  SRR4302541.6783727/94‑1 (MQ=60)
                                    GCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTT                                                      <  SRR4302541.5845549/94‑1 (MQ=60)
                                      AGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAT                                                    <  SRR4302541.3912740/94‑1 (MQ=60)
                                      AACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAT                                                    >  SRR4302541.2726455/1‑94 (MQ=60)
                                      AACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTAT                                                    >  SRR4302541.294497/1‑94 (MQ=60)
                                        CGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCA                                                  >  SRR4302541.1611190/1‑94 (MQ=60)
                                        CGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCA                                                  <  SRR4302541.171694/94‑1 (MQ=60)
                                        CGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCA                                                  <  SRR4302541.4634251/94‑1 (MQ=60)
                                          TCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAAT                                                >  SRR4302541.4821854/1‑94 (MQ=60)
                                          TCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAAT                                                <  SRR4302541.1432136/94‑1 (MQ=60)
                                           CGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATG                                               >  SRR4302541.804694/1‑94 (MQ=60)
                                           CGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATG                                               >  SRR4302541.6215788/1‑94 (MQ=60)
                                           CGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATG                                               >  SRR4302541.3099477/1‑94 (MQ=60)
                                           CGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATG                                               <  SRR4302541.679393/94‑1 (MQ=60)
                                           CGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATG                                               <  SRR4302541.1499869/94‑1 (MQ=60)
                                            GGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGC                                              >  SRR4302541.2331535/1‑94 (MQ=60)
                                                  TTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAA                                        >  SRR4302541.78367/1‑94 (MQ=60)
                                                  TTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAA                                        >  SRR4302541.478538/1‑94 (MQ=60)
                                                  TTCACCAGTGCGCGGACAATCAGCGACAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGGCGAGAA                                        <  SRR4302541.2247807/94‑1 (MQ=60)
                                                   TCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAAT                                       <  SRR4302541.1210353/94‑1 (MQ=60)
                                                    CACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATA                                      >  SRR4302541.5439915/1‑94 (MQ=60)
                                                    CACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATA                                      >  SRR4302541.5679764/1‑94 (MQ=60)
                                                     ACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATAT                                     <  SRR4302541.4802244/94‑1 (MQ=60)
                                                     ACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATAT                                     >  SRR4302541.8124008/1‑94 (MQ=60)
                                                     ACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATAT                                     >  SRR4302541.866056/1‑94 (MQ=60)
                                                      CCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATC                                    <  SRR4302541.6747437/94‑1 (MQ=60)
                                                       CAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCC                                   <  SRR4302541.5127956/94‑1 (MQ=60)
                                                       CAGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCC                                   <  SRR4302541.619274/94‑1 (MQ=60)
                                                        AGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCA                                  <  SRR4302541.2984420/94‑1 (MQ=60)
                                                        AGTGCGCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCA                                  >  SRR4302541.3848800/1‑94 (MQ=60)
                                                             GCGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCAC                             >  SRR4302541.8534597/1‑94 (MQ=60)
                                                              CGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACT                            <  SRR4302541.8048253/94‑1 (MQ=60)
                                                              CGGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACT                            >  SRR4302541.7775843/1‑94 (MQ=60)
                                                               GGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           >  SRR4302541.4127381/1‑94 (MQ=60)
                                                               GGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           <  SRR4302541.5598855/94‑1 (MQ=60)
                                                               GGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           >  SRR4302541.3913411/1‑94 (MQ=60)
                                                               GGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           >  SRR4302541.7268673/1‑94 (MQ=60)
                                                               GGACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTG                           <  SRR4302541.5598857/94‑1 (MQ=60)
                                                                 GCAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCT                         <  SRR4302541.2842470/94‑1 (MQ=60)
                                                                 ACAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCT                         <  SRR4302541.4097146/94‑1 (MQ=60)
                                                                  CAATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTG                        >  SRR4302541.1134971/1‑94 (MQ=60)
                                                                   AATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGGGTTTATCAATGCCGAGAATATCCAGCCACTGCTGC                       >  SRR4302541.2979736/1‑94 (MQ=60)
                                                                   AATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGC                       >  SRR4302541.6820961/1‑94 (MQ=60)
                                                                    ATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCA                      <  SRR4302541.8641077/94‑1 (MQ=60)
                                                                    ATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCA                      <  SRR4302541.8641078/94‑1 (MQ=60)
                                                                    ATCAGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCA                      >  SRR4302541.8834234/1‑94 (MQ=60)
                                                                       AGCGCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCA                   <  SRR4302541.4827620/94‑1 (MQ=60)
                                                                          GCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGCGCGTCAGCCGTGCGGTTATCAATGGCGATAATACCCAGCCACCGCTGCACCACTT                >  SRR4302541.4483416/1‑94 (MQ=60)
                                                                          GCCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTT                >  SRR4302541.975911/1‑94 (MQ=60)
                                                                           CCAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTT               >  SRR4302541.2234195/1‑94 (MQ=60)
                                                                            CAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTT              >  SRR4302541.1948216/1‑94 (MQ=60)
                                                                            CAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTT              <  SRR4302541.6316848/94‑1 (MQ=60)
                                                                            CAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTT              >  SRR4302541.3263105/1‑94 (MQ=60)
                                                                            CAGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTT              >  SRR4302541.7823911/1‑94 (MQ=60)
                                                                             AGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTT             >  SRR4302541.5467986/1‑94 (MQ=60)
                                                                             AGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTT             <  SRR4302541.6923944/94‑1 (MQ=60)
                                                                             AGACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTT             >  SRR4302541.6689789/1‑94 (MQ=60)
                                                                               ACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCCGCCGTGCGTTTATCAATGCCGACAAAATCCAGCCACTGCTGCACCACTTTTTGT           >  SRR4302541.2771856/1‑94 (MQ=60)
                                                                               ACGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGC           >  SRR4302541.8640773/1‑94 (MQ=60)
                                                                                CGCTGCTGCCCCCAGGAAAGACTATGGAGCGGAGCGTCAGCCTTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          <  SRR4302541.5877957/94‑1 (MQ=60)
                                                                                CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          <  SRR4302541.5877959/94‑1 (MQ=60)
                                                                                CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          <  SRR4302541.2054354/94‑1 (MQ=60)
                                                                                CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          <  SRR4302541.600879/94‑1 (MQ=60)
                                                                                CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          >  SRR4302541.6089231/1‑94 (MQ=60)
                                                                                CGCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCT          >  SRR4302541.6089232/1‑94 (MQ=60)
                                                                                 GCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTG         <  SRR4302541.4550215/94‑1 (MQ=60)
                                                                                 GCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTG         >  SRR4302541.3052215/1‑94 (MQ=60)
                                                                                 GCTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTG         >  SRR4302541.8619379/1‑94 (MQ=60)
                                                                                  CTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGG        >  SRR4302541.1727291/1‑94 (MQ=60)
                                                                                  CTGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGG        <  SRR4302541.6111474/94‑1 (MQ=60)
                                                                                   TGCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGC       <  SRR4302541.6357647/94‑1 (MQ=60)
                                                                                    GCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCG      <  SRR4302541.8843254/94‑1 (MQ=60)
                                                                                    GCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCG      <  SRR4302541.7053029/94‑1 (MQ=60)
                                                                                    GCTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCG      <  SRR4302541.7053030/94‑1 (MQ=60)
                                                                                     CTGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGA     >  SRR4302541.4826054/1‑94 (MQ=60)
                                                                                      TGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGAT    >  SRR4302541.2349388/1‑94 (MQ=60)
                                                                                      TGCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTCCTGCACCAGTTTTTGCTGGCGAC    >  SRR4302541.1839031/1‑94 (MQ=60)
                                                                                       GCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATC   >  SRR4302541.1771204/1‑94 (MQ=60)
                                                                                       GCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATC   >  SRR4302541.3117928/1‑94 (MQ=60)
                                                                                       GCCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATC   <  SRR4302541.2896822/94‑1 (MQ=60)
                                                                                        CCCCCAGGAAAGACTAAGAAACGGAGCGTCAGCCATGCGTTTATCAATGCCGAAAATATCCAGCCACTGCTGCACCAGTATTTGCTGGCGATCC  <  SRR4302541.7926458/94‑1 (MQ=60)
                                                                                        |                                                                                             
GCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCC  >  NC_000913/791709‑791890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑