breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 144 A→G intergenic (–/‑46)  / → thrL –/thr operon leader peptide
RA 6,610 G→A R450R (CGC→CGT yaaJ ← inner membrane transport protein
RA 11,000 T→C E119E (GAA→GAG
C92C (TGT→TGC
b0011 ←
htgA →
putative oxidoreductase
HtgA
RA 28,729 C→T A119V (GCC→GTC)  dapB → dihydrodipicolinate reductase
RA 30,594 C→T A315V (GCG→GTG)  carA → carbamoyl‑phosphate synthetase
RA 49,165 T→C V466A (GTG→GCG)  kefC → K+ efflux antiporter glutathione‑regulated
RA 66,528 T→C Q8R (CAG→CGG)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase
MC JC 66,533 3540 bp→27 bp [araD]araB [araD], araA, araB
RA 70,289 G→T intergenic (‑241/‑98) araB ← / → araC L‑ribulokinase/transcriptional regulator for ara operon
RA 78,003 A→C E128A (GAG→GCG)  yabM → putative transport protein
RA 83,095 A→G R145R (CGT→CGC leuA ← 2‑isopropylmalate synthase
RA 87,311 (G)5→6 coding (1682/1725 nt) ilvI → acetolactate synthase isozyme III large subunit
RA 107,212 A→G D219G (GAC→GGC)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
RA 111,615 T→C intergenic (+182/+34) mutT → / ← yacG 7,8‑dihydro‑8‑oxoguanine‑triphosphatase/hypothetical protein
RA 138,226 A→G S382G (AGC→GGC)  yacK → hypothetical protein
RA 138,888 T→C T780A (ACG→GCG)  gcd ← glucose dehydrogenase
RA 147,141 A→G G58G (GGA→GGG yadD → hypothetical protein
RA 148,799 G→A intergenic (‑4/+8) panC ← / ← panB pantothenate synthetase/3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
RA 156,827 A→G G19G (GGT→GGC yadN ← putative fimbrial‑like protein
RA 159,762 A→G R117R (CGT→CGC yadB ← putative tRNA synthetase
RA 171,718 C→T P86L (CCG→CTG)  fhuB → FhuB
RA 182,747 A→G T95T (ACA→ACG yaeG → hypothetical protein
RA 196,422 A→G D213G (GAC→GGC)  cdsA → CDP‑diglyceride synthetase
RA 202,345 T→C V82A (GTA→GCA)  fabZ → (3R)‑hydroxymyristol acyl carrier protein dehydratase
RA 231,829 T→C G236G (GGT→GGC yafD → hypothetical protein
RA 235,988 T→C V5V (GTA→GTG rnhA ← RNase HI
RA 241,745 A→G V532A (GTG→GCG)  yafH ← putative acyl‑CoA dehydrogenase
RA 253,831 T→C Y44H (TAT→CAT)  prfH → putative peptide chain release factor
RA 254,298 T→C T473T (ACA→ACG pepD ← aminoacyl‑histidine dipeptidase
RA 256,955 T→C D143D (GAT→GAC yafA → hypothetical protein
RA 269,425 A→G intergenic (‑19/‑41) perR ← / → yi91a putative transcriptional regulator LYSR‑type/hypothetical protein
RA 272,747 T→C L221P (CTA→CCA)  ykfC → hypothetical protein
RA 276,473 T→C L179L (TTG→CTG)  yagD → YagD
RA 283,609 A→G E395E (GAA→GAG yagF → putative dehydratase
RA 296,697 A→G F107L (TTC→CTC)  yagP ← putative transcriptional regulator LYSR‑type
RA 307,659 T→C T300A (ACC→GCC)  yagX ← hypothetical protein
RA 318,936 C→T G106D (GGC→GAC)  ykgC ← putative oxidoreductase
RA 320,122 T→C A224A (GCT→GCC ykgD → putative ARAC‑type regulatory protein
RA 323,407 A→G G142G (GGA→GGG ykgG → putative transporter
RA 338,761 G→A V405I (GTC→ATC)  yahG → hypothetical protein
RA 363,169 A→G R787R (CGT→CGC lacZ ← beta‑D‑galactosidase
RA 368,911 G→A V359V (GTG→GTA mhpA → 3‑(3‑hydroxyphenyl)propionate hydroxylase
RA 376,616 A→G intergenic (+81/+143) yaiL → / ← yaiM nucleoprotein/polynucleotide‑associated enzyme/putative esterase
JC 379,244 (G)10→7 intergenic (‑118/+47) yaiN ← / ← yaiO putative alpha helix chain/hypothetical protein
RA 379,246 (G)10→11 intergenic (‑120/+47) yaiN ← / ← yaiO putative alpha helix chain/hypothetical protein
RA 394,595 T→C D306G (GAT→GGT)  yaiH ← hypothetical protein
RA 400,205 A→G intergenic (‑58/‑405) ddlA ← / → yaiB D‑alanine‑D‑alanine ligase A/hypothetical protein
RA 418,805 A→G intergenic (+397/‑10) phoR → / → brnQ positive and negative sensor protein for pho regulon/branched chain amino acid transport system II carrier protein
RA 434,599 T→C V80A (GTA→GCA)  nusB → transcription termination
RA 434,687 A→G E109E (GAA→GAG nusB → transcription termination
RA 440,252 A→G L25P (CTG→CCG)  ispA ← geranyltranstransferase
RA 440,417 C→T A51T (GCC→ACC)  xseB ← exonuclease VII small subunit
RA 459,573 A→G I488V (ATT→GTT)  lon → ATP‑dependent protease La
RA 484,107 A→G L246P (CTG→CCG)  acrA ← acridine efflux pump
RA 502,653 C→T intergenic (‑191/+47) ybaL ← / ← fsr putative transport protein/fosmidomycin resistance protein
RA 509,606 (T)6→5 coding (998/2505 nt) ybaR ← putative ATPase
RA 518,231 T→C K48R (AAA→AGA)  ybbO ← putative oxidoreductase
RA 528,763 T→C intergenic (+409/+106) b0501 → / ← b0502 hypothetical protein/hypothetical protein
RA 531,406 C→T A14T (GCT→ACT)  ybbS ← putative transcriptional regulator LYSR‑type
RA 536,980 A→G I87M (ATA→ATG
N42D (AAC→GAC) 
ybbV →
ybbW →
hypothetical protein
putative transport protein
RA 542,052 A→G Q314R (CAG→CGG)  ybbZ → hypothetical protein
RA 547,694 A→G E38E (GAA→GAG b4507 → hypothetical protein
RA 547,835 +G coding (255/261 nt) b4507 → hypothetical protein
RA 552,300 C→T A8A (GCG→GCA purE ← phosphoribosylaminoimidazole carboxylase
RA 556,636 A→G V110A (GTG→GCG)  folD ← 5,10‑methylene‑tetrahydrofolate dehydrogenase
RA 562,546 A→G intergenic (+4/‑7) sfmH → / → sfmF involved in fimbrial asembly/putative fimbrial‑like protein
RA 577,681 (C)6→7 coding (352/462 nt) ybcT → endopeptidase‑like protein
RA 578,027 T→C A30A (GCA→GCG ybcU ← Bor‑like protein
RA 612,751 T→C A238A (GCT→GCC fes → enterochelin esterase
RA 618,600 A→G H375R (CAC→CGC)  fepE → FepE
RA 621,553 A→G S11G (AGC→GGC)  ybdA → putative transport
RA 624,133 T→C V9A (GTA→GCA)  entC → isochorismate hydroxymutase 2
RA 638,137 A→G intergenic (‑281/‑31) dsbG ← / → ahpC thiol:disulfide interchange protein/alkyl hydroperoxide reductase C22 subunit
RA 683,225 A→G H137H (CAT→CAC ybeK ← putative tRNA synthetase
RA 697,801 A→G D200D (GAT→GAC asnB ← asparagine synthetase B
RA 704,130 A→G I322V (ATC→GTC)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC
RA 704,236 T→C L357S (TTG→TCG)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC
RA 704,311 A→G N382S (AAC→AGC)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC
RA 708,421 G→A G289S (GGC→AGC)  ybfM → hypothetical protein
RA 709,321 A→G I103M (ATA→ATG ybfN → hypothetical protein
RA 715,139 T→C intergenic (+10/+31) ybfP → / ← ybfG putative pectinase/hypothetical protein
RA 719,820 A→G L5L (TTA→TTG ybfK → hypothetical protein
RA 729,698 A→G H298R (CAC→CGC)  rhsC → RhsC
RA 765,778 C→T A191V (GCC→GTC)  hrsA → PTS system fructose specific IIABC component
RA 791,797 T→C G405G (GGA→GGG modF ← ModF
RA 831,133 T→C S347P (TCG→CCG)  rhlE → putative ATP‑dependent RNA helicase
RA 844,809 A→G intergenic (‑106/+155) ybiO ← / ← glnQ putative transport protein/ATP‑binding component of glutamine high‑affinity transport system
RA 878,307 A→G T115A (ACT→GCT)  yliI → putative dehydrogenase
RA 888,260 A→G D261D (GAT→GAC b0847 ← putative transport protein
RA 899,581 A→T I73N (ATC→AAC)  artJ ← arginine 3rd transport system periplasmic binding protein
RA 905,792 A→G V74A (GTG→GCG)  b0868 ← putative nucleotide di‑P‑sugar epimerase or dehydratase
RA 914,422 A→G intergenic (‑294/+153) ybjE ← / ← aqpZ putative surface protein/transmembrane water channel
RA 914,564 T→C intergenic (‑436/+11) ybjE ← / ← aqpZ putative surface protein/transmembrane water channel
RA 934,731 A→G Q762R (CAG→CGG)  ftsK → cell division protein
RA 946,689 A→G T144A (ACG→GCG)  b0899 → putative transport
RA 961,423 A→G D69G (GAC→GGC)  rpsA → 30S ribosomal subunit protein S1
RA 962,413 A→G N399S (AAC→AGC)  rpsA → 30S ribosomal subunit protein S1
RA 969,158 C→T R183C (CGC→TGC)  ycaQ → hypothetical protein
RA 981,066 A→T D266V (GAC→GTC)  ycbB → putative amidase
RA 981,566 C→T R433C (CGC→TGC)  ycbB → putative amidase
RA 1,003,383 (G)7→8 coding (184/681 nt) ycbF → putative chaperone
RA 1,015,850 T→C Q558R (CAG→CGG)  b0955 ← putative ATP‑dependent protease
RA 1,016,180 A→G I448T (ATA→ACA)  b0955 ← putative ATP‑dependent protease
RA 1,019,358 T→C intergenic (‑82/+275) ompA ← / ← sulA outer membrane protein 3a/suppressor of lon
RA 1,032,328 A→G T323A (ACC→GCC)  hyaA → hydrogenase‑1 small subunit
RA 1,038,386 T→C L475P (CTG→CCG)  appC → putative third cytochrome oxidase subunit I
RA 1,051,163 Δ1 bp coding (94/231 nt) sfa → Sfa
RA 1,053,820 T→C T518A (ACC→GCC)  torS ← TorS
RA 1,056,041 A→G E186E (GAA→GAG torT → TorT
RA 1,064,328 A→G N357S (AAT→AGT)  yccE → hypothetical protein
RA 1,065,252 T→C F149L (TTT→CTT)  agp → periplasmic glucose‑1‑phosphatase
RA 1,066,017 T→C F404L (TTT→CTT)  agp → periplasmic glucose‑1‑phosphatase
RA 1,074,187 T→C T1307A (ACC→GCC)  putA ← proline dehydrogenase
RA 1,086,330 T→C Y247C (TAC→TGC)  ycdQ ← hypothetical protein
RA 1,090,676 A→G R279R (CGT→CGC ycdS ← putative outer membrane protein
RA 1,098,156 A→G T19A (ACA→GCA)  ycdX → hypothetical protein
RA 1,119,304 T→C N169S (AAC→AGC)  solA ← sarcosine oxidase‑like protein
RA 1,141,015 T→C Q859R (CAG→CGG)  rne ← RNase E
RA 1,142,917 T→C D225G (GAC→GGC)  rne ← RNase E
RA 1,143,101 T→C M164V (ATG→GTG)  rne ← RNase E
RA 1,154,920 C→T P192S (CCG→TCG)  tmk → thymidylate kinase
RA 1,155,780 A→G T266A (ACC→GCC)  holB → DNA polymerase III delta prime subunit
RA 1,156,390 A→G T131A (ACC→GCC)  ycfH → hypothetical protein
RA 1,158,718 T→C Q686R (CAG→CGG)  fhuE ← outer membrane receptor for ferric iron uptake
RA 1,175,607 A→G S320G (AGC→GGC)  ycfU → hypothetical protein
RA 1,192,497 A→G L167P (CTG→CCG)  ycfB ← hypothetical protein
RA 1,199,211 T→C V273V (GTA→GTG intE ← prophage e14 integrase
RA 1,202,871 A→G E131E (GAA→GAG ymfL → hypothetical protein
RA 1,207,165 (G)7→8 coding (442/630 nt) ycfK → hypothetical protein
RA 1,210,786 A→G intergenic (+384/+117) mcrA → / ← ycgW McrA/hypothetical protein
RA 1,212,474 A→G intergenic (‑144/+77) ycgX ← / ← ycgE hypothetical protein/putative transcriptional regulator
RA 1,235,692 T→C Q258R (CAG→CGG)  ycgB ← putative sporulation protein
RA 1,238,083 A→G A430A (GCA→GCG dadA → D‑amino acid dehydrogenase subunit
RA 1,252,715 A→G S821P (TCC→CCC)  b1202 ← putative adhesion and penetration protein
RA 1,302,782 A→G N2S (AAC→AGC)  oppD → OppD
RA 1,329,903 A→G S278G (AGT→GGT)  topA → DNA topoisomerase type I omega protein
RA 1,330,060 A→G D330G (GAC→GGC)  topA → DNA topoisomerase type I omega protein
RA 1,335,418 A→G S522G (AGC→GGC)  acnA → aconitate hydrase 1
RA 1,342,604 G→A A10A (GCC→GCT b4523 ← hypothetical protein
RA 1,348,410 A→G R218R (CGT→CGC fabI ← enoyl‑[acyl‑carrier‑protein] reductase (NADH)
RA 1,354,431 T→C D235G (GAT→GGT)  sapA ← SapA
RA 1,358,270 G→A G221G (GGC→GGT b1297 ← putative glutamine synthetase
RA 1,372,245 A→G S44S (TCA→TCG ycjP → putative transport system permease protein
RA 1,383,611 A→G Q26R (CAG→CGG)  ycjF → hypothetical protein
RA 1,391,027 A→G intergenic (+113/‑203) ycjZ → / → b1329 putative transcriptional regulator LYSR‑type/putative transport periplasmic protein
RA 1,396,204 T→C Q148R (CAG→CGG)  ydaA ← hypothetical protein
RA 1,397,365 A→G G62G (GGT→GGC fnr ← Fnr
RA 1,408,072 A→G T180A (ACG→GCG)  dbpA → ATP‑dependent RNA helicase
RA 1,415,996 (T)6→7 coding (37/171 nt) ydaE ← hypothetical protein
RA 1,442,757 C→T D561D (GAC→GAT ydbH → hypothetical protein
RA 1,445,328 A→G intergenic (‑21/‑215) feaR ← / → feaB FeaR/phenylacetaldehyde dehydrogenase
RA 1,456,536 A→G S83S (TCA→TCG b1394 → hypothetical protein
RA 1,457,617 T→C P180P (CCT→CCC ydbU → hypothetical protein
RA 1,460,324 A→G D59G (GAC→GGC)  b1398 → hypothetical protein
RA 1,492,778 A→G G153G (GGT→GGC b1422 ← putative transcriptional regulator LYSR‑type
RA 1,503,894 T→C T71A (ACC→GCC)  b1433 ← putative membrane transport protein
RA 1,520,878 A→G V71A (GTG→GCG)  b1451 ← putative outer membrane receptor for iron transport
RA 1,520,926 A→G M55T (ATG→ACG)  b1451 ← putative outer membrane receptor for iron transport
RA 1,521,583 A→T I85F (ATC→TTC)  b1452 → putative receptor
RA 1,533,898 A→G intergenic (‑16/+63) yddE ← / ← narV hypothetical protein/cryptic nitrate reductase 2 gamma subunit
RA 1,538,045 G→A P857L (CCG→CTG)  narZ ← cryptic nitrate reductase 2 alpha subunit
RA 1,545,666 A→G D81G (GAC→GGC) 
D81G (GAC→GGC) 
b4525 →
fdnG →
hypothetical protein
formate dehydrogenase‑N, nitrate‑inducible, alpha subunit
RA 1,548,911 T→C C143R (TGT→CGT)  fdnH → formate dehydrogenase‑N, nitrate‑inducible, iron‑sulfur beta subunit
RA 1,571,055 T→C L724L (TTA→TTG pqqL ← putative peptidase
RA 1,603,543 T→C F157L (TTT→CTT)  b1516 → putative LACI‑type transcriptional regulator
RA 1,605,226 G→A T68T (ACG→ACA b1518 → hypothetical protein
RA 1,607,710 A→G L332P (CTT→CCT)  uxaB ← altronate oxidoreductase
RA 1,608,034 A→G V224A (GTG→GCG)  uxaB ← altronate oxidoreductase
RA 1,616,532 G→A T134I (ACC→ATC)  ydeB ← hypothetical protein
RA 1,636,465 T→C intergenic (‑296/+14) b1551 ← / ← cspI hypothetical protein/cold shock‑like protein
RA 1,650,355 T→C F274L (TTC→CTC)  b1579 → putative transposase
RA 1,652,626 T→C E180E (GAA→GAG rspA ← starvation sensing protein
RA 1,668,733 A→G S337S (TCA→TCG ynfM → putative transport protein
RA 1,671,621 A→G intergenic (‑96/‑316) b1600 ← / → b1601 putative chaperone/putative transport protein
RA 1,672,529 T→C V198A (GTG→GCG)  b1601 → putative transport protein
RA 1,672,622 T→C L229P (CTG→CCG)  b1601 → putative transport protein
RA 1,679,869 A→G S62S (AGT→AGC ydgC ← hypothetical protein
RA 1,681,154 T→C S83S (AGT→AGC rstB → sensor histidine protein kinase
RA 1,683,566 (C)6→7 coding (1047/1404 nt) fumC ← fumarase C
RA 1,705,052 T→C V37A (GTA→GCA)  b1629 → putative membrane protein
RA 1,718,191 A→G T98A (ACT→GCT)  slyB → putative outer membrane protein
RA 1,722,918 T→A N247Y (AAC→TAC)  b1647 ← hypothetical protein
RA 1,728,403 T→C R431R (CGT→CGC lhr → Lhr
RA 1,730,968 T→C S1286S (AGT→AGC lhr → Lhr
RA 1,739,051 A→G M373V (ATG→GTG)  ydhC → putative transport protein
RA 1,741,574 2 bp→GA coding (94‑95/1374 nt) ydhE → putative transport protein
RA 1,755,127 T→C V469A (GTC→GCC)  pykF → pyruvate kinase I
RA 1,764,932 A→G M593T (ATG→ACG)  ydiJ ← putative oxidase
RA 1,766,232 T→C T160A (ACC→GCC)  ydiJ ← putative oxidase
RA 1,782,304 A→T D417V (GAT→GTT)  ydiD → putative ligase/synthetase
RA 1,799,252 T→C N448S (AAC→AGC)  thrS ← threonine tRNA synthetase
RA 1,812,482 T→C F198L (TTC→CTC)  katE → catalase
RA 1,846,045 T→C intergenic (‑13/+104) selD ← / ← ydjA selenophosphate synthase/hypothetical protein
RA 1,848,059 T→C V400A (GTT→GCT)  sppA → protease IV
RA 1,852,569 T→C T104A (ACC→GCC)  b1770 ← putative DEOR‑type transcriptional regulator
RA 1,858,723 C→T A212T (GCG→ACG)  b1776 ← putative oxidoreductase
RA 1,860,370 T→C E28E (GAA→GAG yeaA ← hypothetical protein
RA 1,862,301 T→C V143A (GTG→GCG)  yeaD → hypothetical protein
RA 1,870,741 T→C V286A (GTA→GCA)  yeaJ → hypothetical protein
RA 1,885,627 A→G L130P (CTG→CCG)  rnd ← RNase D
RA 1,893,186 T→C S120P (TCA→CCA)  pabB → p‑aminobenzoate synthetase component I
RA 1,899,288 G→A R108W (CGG→TGG)  yoaE ← putative transport protein
RA 1,902,757 T→C L280P (CTG→CCG)  b1819 → PTS enzyme IID, mannose‑specific
RA 1,904,845 T→C E80E (GAA→GAG yebH ← hypothetical protein
RA 1,905,091 T→C intergenic (‑7/+159) yebH ← / ← cspC hypothetical protein/cold shock protein
RA 1,930,950 G→A A560V (GCG→GTG)  edd ← 6‑phosphogluconate dehydratase
RA 1,933,325 A→G G338G (GGT→GGC zwf ← glucose‑6‑phosphate dehydrogenase
RA 1,934,800 T→C L42P (CTG→CCG)  yebK → hypothetical protein
RA 1,973,386 A→G S298S (AGT→AGC motB ← MotB
MC JC 1,976,527 Δ776 bp insB5insA5 insB5, insA5
RA 1,980,940 +C coding (1/363 nt)
coding (627/627 nt)
araH ←
araH ←
ABC‑type arabinose transport system permease component
ABC‑type arabinose transport system permease component
RA 1,981,668 A→G G475G (GGT→GGC araG ← ATP‑binding component of high‑affinity L‑arabinose transport system
RA 1,982,766 C→T P109P (CCG→CCA araG ← ATP‑binding component of high‑affinity L‑arabinose transport system
RA 1,984,089 A→G A21A (GCT→GCC araF ← L‑arabinose‑binding periplasmic protein
RA 1,995,537 T→C T101A (ACG→GCG)  yecC ← putative ATP‑binding component of a transport system
RA 1,995,724 A→G S38S (AGT→AGC yecC ← putative ATP‑binding component of a transport system
RA 2,006,874 A→G Q192R (CAG→CGG)  yedE → putative transport system permease protein
RA 2,008,480 +A coding (637/654 nt) yedK → hypothetical protein
RA 2,025,451 T→C T202A (ACG→GCG)  b1956 ← hypothetical protein
RA 2,027,751 (C)6→7 coding (191/921 nt) yedA → putative transmembrane subunit
RA 2,037,676 (C)5→6 coding (177/1005 nt) b1971 → putative reductase
RA 2,043,272 A→G S130G (AGT→GGT)  b1978 → hypothetical protein
RA 2,049,323 A→G I2147V (ATC→GTC)  b1978 → hypothetical protein
RA 2,069,211 T→C V230A (GTA→GCA)  yeeP → putative histone
RA 2,072,030 T→A C824S (TGC→AGC)  b2000 → outer membrane fluffing protein
RA 2,086,377 T→C E258E (GAA→GAG b2016 ← hypothetical protein
RA 2,116,149 A→G V93A (GTC→GCC)  wcaK ← putative galactokinase
RA 2,132,238 A→G R479R (CGT→CGC b2060 ← hypothetical protein
RA 2,147,941 A→G V356A (GTC→GCC)  b2070 ← putative chaperonin
RA 2,152,717 T→C V276A (GTG→GCG)  b2074 → putative membrane protein
RA 2,154,032 T→C Y250H (TAT→CAT)  yegN → hypothetical protein
RA 2,161,324 G→A V143M (GTG→ATG)  baeS → BaeS
RA 2,191,374 A→G L272P (CTC→CCC)  b2113 ← putative ATPase
RA 2,202,504 (T)7→6 intergenic (+195/‑46) yehK → / → yehL hypothetical protein/hypothetical protein
RA 2,210,202 A→G D5D (GAT→GAC yehS ← hypothetical protein
RA 2,216,746 G→C S252S (TCC→TCG yehZ ← putative transport system permease protein
RA 2,219,147 T→C H288R (CAC→CGC)  bglX ← beta‑D‑glucoside glucohydrolase
RA 2,223,035 A→G intergenic (‑136/+29) pbpG ← / ← yohC penicillin‑binding protein 7/hypothetical protein
RA 2,234,921 T→C T285A (ACG→GCG)  mglC ← MglC
RA 2,239,132 T→C H186R (CAC→CGC)  galS ← mgl repressor
RA 2,252,917 C→T R97H (CGC→CAC)  yeiK ← hypothetical protein
RA 2,258,992 C→T A147T (GCG→ACG)  fruA ← PTS system, fructose‑specific transport protein
RA 2,265,000 (G)5→6 coding (736/1467 nt) yeiQ → putative oxidoreductase
RA 2,272,980 T→C V261A (GTG→GCG)  yejB → putative transport system permease protein
RA 2,295,407 C→T G84G (GGG→GGA ccmA ← ATP binding protein of heme exporter A
RA 2,315,444 G→C A802G (GCG→GGG)  rcsC ← RcsC
RA 2,327,579 T→C Y80C (TAC→TGC)  b2226 ← hypothetical protein
RA 2,359,592 T→C T214A (ACC→GCC)  b2248 ← putative regulator
RA 2,417,173 A→G S8S (AGT→AGC b2299 ← putative regulator
RA 2,429,483 T→C E65E (GAA→GAG dedD ← putative lipoprotein
RA 2,438,697 A→C L310R (CTG→CGG)  fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,441,613 T→C F610F (TTT→TTC b2324 → putative peptidase
RA 2,453,196 A→G T157T (ACT→ACC b2339 ← putative fimbrial‑like protein
RA 2,486,394 T→C A278A (GCA→GCG yfdE ← hypothetical protein
RA 2,497,463 T→C V258A (GTG→GCG)  b2380 → putative sensor protein
RA 2,506,868 A→G P193P (CCT→CCC glk ← glucokinase
RA 2,551,460 T→C A72A (GCT→GCC hemF → coproporphyrinogen III oxidase
RA 2,578,097 A→G T148A (ACC→GCC)  tktB → transketolase 2 isozyme
RA 2,594,634 T→C I42V (ATA→GTA)  b2475 ← hypothetical protein
RA 2,616,953 T→C S410G (AGT→GGT)  uraA ← UraA
RA 2,621,256 G→A E65K (GAA→AAA)  ppk → polyphosphate kinase
RA 2,637,648 T→C V316V (GTA→GTG hisS ← histidine tRNA synthetase
RA 2,649,291 +C coding (1017/4962 nt) b2520 ← hypothetical protein
RA 2,654,241 A→G R46R (CGT→CGC b2523 ← putative peptidase
RA 2,672,460 G→A H83H (CAC→CAT yphB ← hypothetical protein
RA 2,686,480 T→C Q115R (CAG→CGG)  yfhA ← putative 2‑component transcriptional regulator
RA 2,691,024 A→T F847Y (TTT→TAT)  purL ← phosphoribosylformyl‑glycineamide synthetase
RA 2,701,551 T→C Q178R (CAG→CGG)  rnc ← RNase III
RA 2,711,990 C→T L359L (CTG→TTG)  srmB → ATP‑dependent RNA helicase
RA 2,719,936 A→G Q655R (CAG→CGG)  yfiQ → hypothetical protein
RA 2,729,373 +C intergenic (‑195/+247) rrsG ← / ← clpB 16S ribosomal RNA/ClpB
RA 2,744,399 T→C intergenic (‑194/+55) rpsP ← / ← ffh 30S ribosomal subunit protein S16/FFh
RA 2,747,389 Δ1 bp coding (572/591 nt) b4540 → hypothetical protein
RA 2,751,091 G→C A426P (GCT→CCT)  recN → RecN
RA 2,765,466 (C)7→8 intergenic (+90/‑265) yfjO → / → b2632 hypothetical protein/putative GTP‑binding protein
RA 2,766,382 C→G P218A (CCC→GCC)  b2632 → putative GTP‑binding protein
RA 2,766,423 G→A R231R (CGG→CGA b2632 → putative GTP‑binding protein
RA 2,766,427 2 bp→AC coding (697‑698/864 nt) b2632 → putative GTP‑binding protein
RA 2,766,473 A→G D248G (GAT→GGT)  b2632 → putative GTP‑binding protein
RA 2,766,475 A→G T249A (ACC→GCC)  b2632 → putative GTP‑binding protein
RA 2,781,020 +G intergenic (‑144/+65) ypjA ← / ← b2648 putative ATP‑binding component of a transport system/hypothetical protein
RA 2,781,027 C→G intergenic (‑151/+58) ypjA ← / ← b2648 putative ATP‑binding component of a transport system/hypothetical protein
RA 2,785,180 Δ1 bp coding (413/495 nt) b2655 → hypothetical protein
RA 2,796,560 T→C intergenic (‑44/‑625) stpA ← / → b2670 StpA/hypothetical protein
RA 2,796,565 T→C intergenic (‑49/‑620) stpA ← / → b2670 StpA/hypothetical protein
RA 2,805,526 A→G D125G (GAC→GGC)  proX → high‑affinity transport system for glycine betaine and proline
RA 2,812,859 Δ1 bp intergenic (‑105/+46) ygaG ← / ← gshA hypothetical protein/gamma‑glutamate‑cysteine ligase
RA 2,819,446 G→C G196G (GGC→GGG alaS ← alanyl‑tRNA synthetase
RA 2,820,144 Δ1 bp intergenic (+24/+18) b4542 → / ← oraA hypothetical protein/OraA
RA 2,823,915 A→G K21E (AAG→GAG)  srlA → PTS system, glucitol/sorbitol‑specific IIC component
RA 2,824,797 T→C F128S (TTT→TCT)  srlE → PTS system glucitol/sorbitol‑specific enzyme IIBC component
RA 2,825,102 T→C F230L (TTC→CTC)  srlE → PTS system glucitol/sorbitol‑specific enzyme IIBC component
RA 2,830,091 T→C P99P (CCA→CCG ygaA ← putative 2‑component transcriptional regulator
RA 2,838,044 +G coding (498/1458 nt) ascF → PTS system enzyme IIBC
RA 2,838,055 Δ1 bp coding (509/1458 nt) ascF → PTS system enzyme IIBC
RA 2,842,033 G→T R67R (CGC→CGA hycG ← hydrogenase activity
RA 2,854,119 C→T R587C (CGC→TGC)  fhlA → FhlA
RA 2,865,091 T→C T162A (ACC→GCC)  rpoS ← sigma S factor
RA 2,871,779 A→G D79D (GAT→GAC cysC ← adenosine 5'‑phosphosulfate kinase
RA 2,872,811 T→C D211G (GAC→GGC)  cysN ← ATP‑sulfurylase subunit 1
RA 2,877,536 G→C A87G (GCT→GGT)  b2755 ← hypothetical protein
RA 2,888,375 A→G L516P (CTT→CCT)  cysJ ← sulfite reductase (NADPH)
RA 2,896,819 +C coding (622/657 nt) ygcU ← putative oxidoreductase subunit
RA 2,917,193 A→G V72A (GTG→GCG)  ygcX ← putative glucarate dehydratase
RA 2,923,951 T→C H194H (CAT→CAC yqcD → hypothetical protein
RA 2,926,421 T→C A57A (GCT→GCC sdaC → putative serine transporter
RA 2,929,074 A→G intergenic (+109/‑3) sdaB → / → b2798 L‑serine dehydratase/Exo
RA 2,956,046 A→C R287R (CGT→CGG ptr ← protease III
RA 2,961,511 C→T G76G (GGG→GGA ppdB ← prepilin peptidase dependent protein B
RA 2,963,770 T→C D97G (GAC→GGC)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
RA 2,967,341 T→C intergenic (‑342/‑343) ygdP ← / → mutH putative invasion protein/methyl‑directed mismatch repair
RA 2,968,005 T→A W108R (TGG→AGG)  mutH → methyl‑directed mismatch repair
RA 2,996,557 T→C K112K (AAA→AAG b2863 ← hypothetical protein
RA 3,005,399 +C coding (1044/1092 nt) ygeW → putative carbamoyl transferase
RA 3,007,730 T→C W316R (TGG→CGG)  ygeY → putative deacetylase
RA 3,012,261 A→G intergenic (‑1/+47) b2875 ← / ← b2876 putative synthases/hypothetical protein
RA 3,013,656 T→C M159T (ATG→ACG)  ygfJ → hypothetical protein
RA 3,014,968 G→A M296I (ATG→ATA b2878 → putative oxidoreductase Fe‑S subunit
RA 3,025,676 +C coding (535/570 nt) ygfQ → hypothetical protein
RA 3,075,220 (T)5→6 coding (256/357 nt) yggP ← hypothetical protein
RA 3,077,340 T→C S4G (AGT→GGT)  cmtB ← PTS system mannitol‑specific enzyme IIA component
RA 3,077,514 Δ1 bp intergenic (‑165/+149) cmtB ← / ← tktA PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
RA 3,077,640 +G intergenic (‑291/+23) cmtB ← / ← tktA PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
RA 3,077,905 A→G S584P (TCG→CCG)  tktA ← transketolase 1 isozyme
RA 3,078,245 C→G G470G (GGG→GGC tktA ← transketolase 1 isozyme
RA 3,082,631 C→T G434S (GGC→AGC)  speA ← biosynthetic arginine decarboxylase
RA 3,093,303 +G coding (187/705 nt) yggS → hypothetical protein
RA 3,099,661 (A)5→6 intergenic (‑19/+164) yggN ← / ← b2959 hypothetical protein/hypothetical protein
RA 3,107,469 +C intergenic (‑296/‑101) b2965 ← / → yqgA ornithine decarboxylase isozyme/putative transport protein
RA 3,108,438 C→A noncoding (56/76 nt) pheV → tRNA‑Phe
RA 3,115,116 +C coding (2013/2028 nt) b2974 ← putative endoglucanase
RA 3,116,492 C→T E213K (GAA→AAA)  b2974 ← putative endoglucanase
RA 3,123,585 +G coding (953/2286 nt) glcF ← glycolate oxidase iron‑sulfur subunit
RA 3,130,334 A→G V30V (GTT→GTC b2983 ← hypothetical protein
RA 3,132,550 T→C G135G (GGT→GGC yghT → hypothetical protein
RA 3,142,620 T→C Q179R (CAG→CGG)  hybA ← hydrogenase‑2 small subunit
RA 3,150,581 T→C Y111H (TAT→CAT)  metC → cystathionine beta‑lyase
RA 3,151,557 C→T intergenic (+119/‑21) metC → / → yghB cystathionine beta‑lyase/hypothetical protein
RA 3,152,247 +C intergenic (+10/+29) yghB → / ← yqhC hypothetical protein/putative ARAC‑type regulatory protein
JC 3,156,257 (CCA)2→1 coding (594‑596/930 nt) yqhG → hypothetical protein
RA 3,158,202 +C coding (961/975 nt) ygiRQ ← Fe‑S oxidoreductase
RA 3,158,281 C→T L294L (CTG→CTA ygiRQ ← Fe‑S oxidoreductase
RA 3,166,935 2 bp→CG coding (312‑313/483 nt) b3023 ← hypothetical protein
RA 3,167,940 G→A G33S (GGT→AGT)  qseB → quorum sensing regulator B
RA 3,185,776 T→C F121L (TTC→CTC)  yqiG → putative membrane protein
RA 3,185,807 T→C F131S (TTT→TCT)  yqiG → putative membrane protein
RA 3,186,925 A→G T504A (ACT→GCT)  yqiG → putative membrane protein
RA 3,195,844 A→G V480A (GTG→GCG)  glnE ← GlnE
RA 3,206,609 C→T S315S (AGC→AGT ygjE → hypothetical protein
RA 3,206,988 A→G T442A (ACC→GCC)  ygjE → hypothetical protein
RA 3,208,504 T→C V28A (GTT→GCT)  rpsU → 30S ribosomal subunit protein S21
RA 3,221,097 2 bp→GC coding (824‑825/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit
RA 3,221,392 T→C R373R (CGT→CGC b3076 → evolved beta‑D‑galactosidase alpha subunit
RA 3,222,061 A→G K596K (AAA→AAG b3076 → evolved beta‑D‑galactosidase alpha subunit
RA 3,222,944 4 bp→AGCA coding (2671‑2674/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit
RA 3,224,879 Δ1 bp coding (1005/1434 nt) ygjI → putative oxidoreductase
RA 3,224,926 +C coding (1052/1434 nt) ygjI → putative oxidoreductase
RA 3,225,194 C→T V440V (GTC→GTT ygjI → putative oxidoreductase
RA 3,236,177 Δ1 bp intergenic (+239/‑45) ygjR → / → ygjT hypothetical protein/putative transport protein
RA 3,236,735 +C coding (514/966 nt) ygjT → putative transport protein
RA 3,236,758 Δ1 bp coding (537/966 nt) ygjT → putative transport protein
RA 3,237,242 +C intergenic (+55/‑342) ygjT → / → ygjU putative transport protein/putative transport protein
RA 3,237,247 +C intergenic (+60/‑337) ygjT → / → ygjU putative transport protein/putative transport protein
RA 3,240,123 T→C M278V (ATG→GTG)  uxaA ← altronate hydrolase
RA 3,242,993 A→G T84A (ACC→GCC)  exuT → ExuT
RA 3,250,165 C→T R74R (CGC→CGT yhaH → putative cytochrome
RA 3,253,742 Δ1 bp coding (551/552 nt) yhaNM ← hypothetical protein
RA 3,255,594 Δ1 bp coding (4/1278 nt) yhaO ← putative transport system permease protein
RA 3,255,616 Δ1 bp intergenic (‑19/+312) yhaO ← / ← yhaP putative transport system permease protein/putative L‑serine dehydratase
RA 3,256,893 +C coding (405/423 nt) yhaQ ← putative L‑serine dehydratase
RA 3,257,838 +G coding (2222/2241 nt) tdcE ← putative formate acetyltransferase 3
RA 3,269,236 G→C A59G (GCT→GGT)  b3124 ← hypothetical protein
RA 3,278,453 T→C L38L (TTG→CTG)  agaW → PTS system N‑acetylgalactosameine‑specific IIC component 2
RA 3,279,197 A→C R145S (AGA→AGC agaA → putative N‑acetylgalactosamine‑6‑phosphate deacetylase
RA 3,291,253 T→C R71R (CGT→CGC yraM → putative glycosylase
RA 3,302,454 (C)7→8 coding (1005/1245 nt) mtr ← tryptophan‑specific transport protein
RA 3,304,497 A→G R352R (CGT→CGC deaD ← inducible ATP‑independent RNA helicase
RA 3,310,422 T→C D133G (GAC→GGC)  rbfA ← ribosome‑binding factor A
RA 3,312,106 T→C Y517C (TAC→TGC)  infB ← protein chain initiation factor IF‑2
RA 3,319,717 A→G noncoding (83/87 nt) leuU ← tRNA‑Leu
RA 3,345,093 A→G E113G (GAA→GGA)  yhbJ → hypothetical protein
RA 3,352,254 2 bp→GC intergenic (‑569/‑12) yhcC ← / → gltB hypothetical protein/glutamate synthase large subunit
RA 3,362,606 A→G D722G (GAC→GGC)  yhcD → putative outer membrane protein
RA 3,389,812 Δ1 bp intergenic (‑149/+4151) tldD ← / ← cafA TldD/CafA
RA 3,393,106 Δ1 bp intergenic (‑3443/+857) tldD ← / ← cafA TldD/CafA
RA 3,406,487 T→C L415P (CTT→CCT)  b3258 → sodium/pantothenate symporter
RA 3,418,840 +G coding (1070/1089 nt) b3269 → putative transport system permease protein
RA 3,421,120 Δ1 bp noncoding (60/121 nt) rrfF ← 5S ribosomal RNA
RA 3,429,527 Δ1 bp coding (40/510 nt) yrdD ← putative DNA topoisomerase
RA 3,430,453 +C coding (745/747 nt) smf ← putative Rossmann‑fold nucleotide‑binding protein
RA 3,432,762 T→C R304R (CGT→CGC fmt → 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
RA 3,446,406 G→A T134M (ACG→ATG)  rplP ← 50S ribosomal subunit protein L16
RA 3,446,647 T→C T54A (ACC→GCC)  rplP ← 50S ribosomal subunit protein L16
RA 3,455,292 T→C W427R (TGG→CGG)  b3325 → YheF
RA 3,458,811 C→T A51V (GCG→GTG)  hofG → putative general protein secretion protein
RA 3,464,165 T→C E66E (GAA→GAG bfr ← bacterioferrin
RA 3,471,916 C→T G92S (GGT→AGT)  rpsL ← 30S ribosomal subunit protein S12
RA 3,477,397 T→C N283S (AAC→AGC)  kefB ← KefB
RA 3,483,905 T→C V50A (GTG→GCG)  crp → cyclic AMP receptor protein
RA 3,494,555 T→C intergenic (+41/‑85) nirD → / → nirC nitrite reductase (NAD(P)H) subunit/Nitrite transporter
RA 3,500,011 G→A R12Q (CGG→CAG)  b3372 → hypothetical protein
RA 3,500,309 Δ1 bp coding (333/363 nt) b3372 → hypothetical protein
RA 3,506,318 (C)5→6 coding (1134/1227 nt) yhfW ← putative mutase
RA 3,509,198 A→G V189A (GTG→GCG)  yhfZ ← hypothetical protein
RA 3,509,958 Δ1 bp intergenic (‑195/+314) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase
RA 3,510,121 +G intergenic (‑358/+151) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase
RA 3,522,805 A→G Y774C (TAC→TGC)  mrcA → penicillin‑binding protein 1A
RA 3,537,405 +GC intergenic (+61/‑394) b3407 → / → feoA hypothetical protein/ferrous iron transport protein A
RA 3,542,788 A→G S107G (AGC→GGC)  yhgH → hypothetical protein
RA 3,544,357 +G coding (163/1314 nt) gntT → gluconate permease
RA 3,544,360 +G coding (166/1314 nt) gntT → gluconate permease
RA 3,544,363 +C coding (169/1314 nt) gntT → gluconate permease
RA 3,548,005 C→T G701D (GGT→GAT)  malP ← maltodextrin phosphorylase
RA 3,554,088 +C coding (2/624 nt) yhgKJ ← RNA phosphage cyclase
RA 3,554,113 Δ1 bp coding (372/396 nt) yhgKJ ← RNA phosphage cyclase
RA 3,554,118 +C coding (367/396 nt) yhgKJ ← RNA phosphage cyclase
RA 3,558,932 2 bp→GC coding (153‑154/831 nt) glpG ← GlpG
RA 3,573,700 +C intergenic (+403/+997) yhgN → / ← gntK hypothetical protein/gluconokinase 2
RA 3,575,446 +G coding (912/942 nt) gntR ← GntR
RA 3,579,035 T→C R89R (CGT→CGC yhhY → hypothetical protein
RA 3,580,138 A→G L215L (CTA→CTG yhhZ → hypothetical protein
RA 3,609,245 T→C L54L (TTA→TTG yhhS ← putative transport
RA 3,610,653 A→G P18P (CCA→CCG yhhU → hypothetical protein
RA 3,621,612 T→C intergenic (+199/‑397) yhhH → / → yhhI hypothetical protein/putative receptor
RA 3,624,949 C→T E724K (GAA→AAA)  yhiH ← putative ATP‑binding component of a transport system, fragment 1
RA 3,627,142 +G intergenic (‑24/+23) yhiH ← / ← yhiI putative ATP‑binding component of a transport system, fragment 1/putative membrane protein
RA 3,629,781 A→G V147A (GTT→GCT)  yhiJ ← hypothetical protein
RA 3,654,259 T→C T38A (ACC→GCC)  hdeA ← hypothetical protein
RA 3,670,236 T→C Y89H (TAT→CAT)  yhjC → putative transcriptional regulator LYSR‑type
RA 3,675,076 +G coding (920/2076 nt) yhjG ← hypothetical protein
RA 3,677,270 T→C T147T (ACT→ACC kdgK → ketodeoxygluconokinase
RA 3,682,619 A→G R199R (CGT→CGC yhjK ← hypothetical protein
RA 3,686,775 +T intergenic (‑23/+9) b3530 ← / ← yhjM putative oxidoreductase subunit/putative endoglucanase
RA 3,703,602 2 bp→GC intergenic (‑183/+124) dppB ← / ← dppA dipeptide transport system permease protein 1/dipeptide transport protein
RA 3,714,436 2 bp→GC coding (261‑262/660 nt) yiaD → putative outer membrane protein
RA 3,734,264 2 bp→GC coding (542‑543/825 nt) bax ← putative ATP‑binding protein
RA 3,735,666 T→C S181P (TCC→CCC)  malS → alpha‑amylase
RA 3,737,956 2 bp→CG coding (623‑624/1254 nt) avtA → alanine‑alpha‑ketoisovalerate transaminase
RA 3,738,723 A→G intergenic (+136/+15) avtA → / ← yiaI alanine‑alpha‑ketoisovalerate transaminase/hypothetical protein
RA 3,743,603 Δ1 bp coding (1171/1275 nt) yiaN → putative membrane protein
RA 3,754,310 C→A R382M (AGG→ATG)  yiaY ← putative oxidoreductase
JC 3,754,317 +GCG coding (1138/1149 nt) yiaY ← putative oxidoreductase
RA 3,758,509 T→C D123G (GAC→GGC)  selA ← selenocysteine synthase
RA 3,764,283 T→C V107A (GTT→GCT)  yibA → hypothetical protein
RA 3,765,203 A→T Q119L (CAG→CTG)  yibJ → hypothetical protein
RA 3,765,234 G→A G129G (GGG→GGA yibJ → hypothetical protein
RA 3,766,847 T→C intergenic (+582/‑125) yibG → / → b4549 hypothetical protein/hypothetical protein
RA 3,771,793 T→C V629A (GTG→GCG)  mtlA → PTS system, mannitol‑specific enzyme IIABC components
RA 3,778,301 T→C V283A (GTA→GCA)  lldD → L‑lactate dehydrogenase
RA 3,782,476 A→G V56A (GTT→GCT)  yibN ← hypothetical protein
RA 3,783,479 A→G D198G (GAT→GGT)  yibO → putative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
RA 3,788,398 A→G V192A (GTG→GCG)  tdh ← threonine dehydrogenase
RA 3,816,074 A→G T234A (ACC→GCC)  dinD → DNA‑damage‑inducible protein
RA 3,825,188 T→C M369V (ATG→GTG)  gltS ← glutamate transport
RA 3,840,766 G→A C243C (TGC→TGT yicO ← hypothetical protein
RA 3,853,551 T→C Q16R (CAG→CGG)  yidG ← hypothetical protein
RA 3,854,698 T→C Y445C (TAC→TGC)  yidJ ← putative sulfatase
RA 3,857,430 A→G I105T (ATC→ACC)  yidK ← putative cotransporter
RA 3,859,397 A→G V73A (GTG→GCG)  glvG ← putative 6‑phospho‑beta‑glucosidase
RA 3,866,583 A→G S119S (AGT→AGC yidR ← hypothetical protein
RA 3,866,718 T→C A74A (GCA→GCG yidR ← hypothetical protein
RA 3,870,631 Δ1 bp coding (610/1764 nt) dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
RA 3,870,765 +G coding (476/1764 nt) dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
RA 3,870,876 Δ1 bp coding (365/1764 nt) dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
RA 3,872,450 +G coding (338/387 nt) yidW ← regulator protein for dgo operon
RA 3,873,627 Δ1 bp coding (506/672 nt) yidX → putative replicase
RA 3,873,986 T→C T199A (ACG→GCG)  yidA ← hypothetical protein
RA 3,876,595 G→C P385A (CCG→GCG)  gyrB ← DNA gyrase subunit B
RA 3,878,432 A→G C140R (TGC→CGC)  recF ← RecF
RA 3,881,483 Δ1 bp intergenic (‑126/‑482) dnaA ← / → rpmH DnaA/50S ribosomal subunit protein L34
RA 3,887,809 A→G intergenic (+35/‑56) b3708 → / → tnaB tryptophanase/low affinity tryptophan permease
RA 3,893,920 C→T G107S (GGC→AGC)  yieG ← putative membrane/transport protein
RA 3,896,324 +G coding (698/711 nt) b3718 ← putative isomerase
RA 3,896,435 G→A A196V (GCG→GTG)  b3718 ← putative isomerase
RA 3,899,440 T→C T137A (ACC→GCC)  yieC ← putative receptor protein
RA 3,923,014 A→G I83T (ATA→ACA)  gidA ← glucose‑inhibited division
RA 3,927,062 +G coding (2/1284 nt)
coding (169/183 nt)
yieM ←
b4564 ←
hypothetical protein
hypothetical protein
RA 3,929,523 T→C V194A (GTG→GCG)  kup → low affinity potassium transport system
RA 3,930,457 Δ1 bp coding (1515/1560 nt) kup → low affinity potassium transport system
RA 3,930,568 +G intergenic (+66/‑373) kup → / → rbsD low affinity potassium transport system/RbsD
RA 3,930,677 +G intergenic (+175/‑264) kup → / → rbsD low affinity potassium transport system/RbsD
RA 3,935,895 A→G T15A (ACC→GCC)  rbsR → RbsR
RA 3,937,900 G→A G113G (GGC→GGT yieO ← putative transport protein
JC 3,938,587 +TGC coding (220/546 nt) yiePs ← hypothetical protein
RA 3,941,734 Δ1 bp noncoding (409/2906 nt) rrlC → 23S ribosomal RNA
RA 3,944,244 C→A intergenic (+13/‑80) rrlC → / → rrfC 23S ribosomal RNA/5S ribosomal RNA
RA 3,944,676 2 bp→GC intergenic (+20/+75) trpT → / ← yifDA tRNA‑Trp/transcription regulator
RA 3,945,351 +C coding (1/600 nt)
coding (240/240 nt)
yifDA ←
yifDA ←
transcription regulator
transcription regulator
RA 3,950,557 2 bp→GC coding (451‑452/930 nt) ilvE → branched‑chain amino‑acid aminotransferase
RA 3,951,166 +C coding (35/1818 nt) ilvD → dihydroxyacid dehydratase
RA 3,951,168 +G coding (37/1818 nt) ilvD → dihydroxyacid dehydratase
RA 3,957,092 +CC intergenic (+26/+58) ilvC → / ← ppiC ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
RA 3,957,124 +G intergenic (+58/+26) ilvC → / ← ppiC ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
RA 3,957,548 1 bp→GG intergenic (‑117/‑744) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/rep helicase
RA 3,957,551 +T intergenic (‑120/‑741) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/rep helicase
RA 3,957,560 +C intergenic (‑129/‑732) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/rep helicase
RA 3,958,877 2 bp→CG coding (586‑587/2022 nt) rep → rep helicase
RA 3,960,578 2 bp→CG coding (1266‑1267/1485 nt) gppA ← guanosine pentaphosphatase
RA 3,961,588 C→G R86T (AGG→ACG)  gppA ← guanosine pentaphosphatase
RA 3,964,491 A→G T154A (ACT→GCT)  rho → transcription termination factor Rho
RA 3,967,593 A→G K317K (AAA→AAG wzzE → putative transport protein
JC 3,968,317 +CGT coding (612/1170 nt) wecB → UDP‑N‑acetyl glucosamine ‑2‑epimerase
RA 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
RA 3,971,075 2 bp→GC coding (942‑943/1068 nt) rffG → dTDP‑glucose 4,6‑dehydratase
RA 3,972,637 2 bp→GC coding (430‑431/546 nt) wecD → hypothetical protein
RA 3,975,313 Δ1 bp intergenic (+173/‑901) wzxE → / → wecF putative cytochrome/TDP‑Fuc4NAc:lipidII transferase
RA 3,978,702 C→T S68F (TCC→TTC)  yifK → putative amino acid/amine transport protein
RA 3,979,535 +G coding (1036/1386 nt) yifK → putative amino acid/amine transport protein
RA 3,979,546 Δ1 bp coding (1047/1386 nt) yifK → putative amino acid/amine transport protein
RA 3,980,297 (C)8→9 noncoding (79/87 nt) leuT → tRNA‑Leu
RA 3,980,918 2 bp→CG coding (348‑349/1236 nt) aslB → putative arylsulfatase regulator
RA 3,987,586 2 bp→GC coding (814‑815/963 nt) hemC ← porphobilinogen deaminase
RA 3,987,822 2 bp→CG coding (578‑579/963 nt) hemC ← porphobilinogen deaminase
RA 3,998,290 +G coding (379/486 nt) b4566 ← hypothetical protein
RA 4,001,421 2 bp→GC coding (381‑382/903 nt) rarD ← hypothetical protein
RA 4,001,486 2 bp→GC coding (316‑317/903 nt) rarD ← hypothetical protein
RA 4,001,717 +C coding (86/903 nt) rarD ← hypothetical protein
RA 4,004,979 +G coding (1511/1833 nt) recQ → ATP‑dependent DNA helicase
RA 4,004,984 +CG coding (1516/1833 nt) recQ → ATP‑dependent DNA helicase
RA 4,005,601 +C coding (237/282 nt) b4568 → hypothetical protein
RA 4,006,483 +C coding (183/207 nt) b4554 ← hypothetical protein
RA 4,007,718 2 bp→CG coding (943‑944/1023 nt) pldB → lysophospholipase L(2)
RA 4,007,907 +GG intergenic (+109/‑11) pldB → / → b4555 lysophospholipase L(2)/hypothetical protein
RA 4,008,375 +A coding (458/516 nt) b4555 → hypothetical protein
RA 4,008,391 +C coding (474/516 nt) b4555 → hypothetical protein
RA 4,008,393 C→G A159G (GCT→GGT)  b4555 → hypothetical protein
RA 4,008,400 +GAAA coding (483/516 nt) b4555 → hypothetical protein
RA 4,008,404 +G coding (487/516 nt) b4555 → hypothetical protein
RA 4,008,411 +C coding (494/516 nt) b4555 → hypothetical protein
RA 4,008,421 +T coding (504/516 nt) b4555 → hypothetical protein
RA 4,008,429 +TG coding (512/516 nt) b4555 → hypothetical protein
RA 4,008,469 +G intergenic (+36/‑197) b4555 → / → yigM hypothetical protein/hypothetical protein
RA 4,008,473 +G intergenic (+40/‑193) b4555 → / → yigM hypothetical protein/hypothetical protein
RA 4,008,491 2 bp→CG intergenic (+58/‑174) b4555 → / → yigM hypothetical protein/hypothetical protein
RA 4,008,525 +G intergenic (+92/‑141) b4555 → / → yigM hypothetical protein/hypothetical protein
RA 4,013,176 C→G G182R (GGA→CGA)  ysgA ← hypothetical protein
RA 4,013,177 +C coding (543/999 nt) ysgA ← hypothetical protein
RA 4,013,717 +G coding (3/999 nt) ysgA ← hypothetical protein
RA 4,013,772 +C intergenic (‑53/‑246) ysgA ← / → udp hypothetical protein/uridine phosphorylase
RA 4,016,743 C→T P101L (CCC→CTC)  ubiE → UbiE
RA 4,019,857 C→T intergenic (+56/‑28) b3836 → / → b3838 hypothetical protein/hypothetical protein
RA 4,019,860 Δ2 bp intergenic (+59/‑24) b3836 → / → b3838 hypothetical protein/hypothetical protein
RA 4,021,684 Δ1 bp coding (542/609 nt)|coding (1/243 nt) b4556 →
tatD →
hypothetical protein
Mg‑dependent DNase
RA 4,026,252 2 bp→GC coding (110‑111/1164 nt) fadA ← thiolase I
RA 4,031,984 +C coding (1250/1299 nt) trkH → TrkH
RA 4,039,349 T→C P77P (CCA→CCG mobA ← MobA
RA 4,039,960 Δ1 bp intergenic (+42/‑36) yihD → / → yihE hypothetical protein/hypothetical protein
RA 4,041,654 G→A intergenic (+29/‑125) dsbA → / → b3861 protein disulfide isomerase I/putative GTP‑binding protein
RA 4,041,671 (T)6→7 intergenic (+46/‑108) dsbA → / → b3861 protein disulfide isomerase I/putative GTP‑binding protein
RA 4,051,144 Δ1 bp intergenic (+146/+305) hemN → / ← glnG O2‑independent coproporphyrinogen III oxidase/GlnG
RA 4,051,170 +C intergenic (+172/+279) hemN → / ← glnG O2‑independent coproporphyrinogen III oxidase/GlnG
RA 4,057,759 +C coding (1773/1776 nt) yihK → putative GTP‑binding factor
RA 4,057,859 (T)9→8 intergenic (+97/‑167) yihK → / → yihL putative GTP‑binding factor/putative transcriptional regulator
RA 4,058,326 A→G T101A (ACC→GCC)  yihL → putative transcriptional regulator
RA 4,059,768 A→G intergenic (+44/‑58) yihM → / → yihN hypothetical protein/putative resistance protein
RA 4,061,961 Δ1 bp coding (1386/1458 nt) b3876 ← putative permease
RA 4,066,222 T→C Y212C (TAC→TGC)  yihQ ← putative glycosidase
RA 4,071,652 A→G S114G (AGC→GGC)  yihV → putative kinase
RA 4,085,675 Δ1 bp intergenic (+38/+13) yiiG → / ← frvR hypothetical protein/putative frv operon regulatory protein
RA 4,088,793 T→C T385T (ACA→ACG frvB ← PTS system, fructose‑like enzyme IIBC component
RA 4,091,351 C→T G168D (GGC→GAC)  rhaD ← rhamnulose‑phosphate aldolase
MC JC 4,091,572 3461 bp→29 bp [rhaD]rhaB [rhaD], rhaA, rhaB
RA 4,103,519 +T intergenic (‑268/‑530) cpxR ← / → yiiP transcriptional regulator in 2‑component system/putative transport system permease protein
RA 4,108,732 T→C Q119R (CAG→CGG)  tpiA ← triosephosphate isomerase
RA 4,109,226 A→G L190P (CTA→CCA)  yiiQ ← hypothetical protein
RA 4,114,335 T→C A156A (GCA→GCG glpK ← glycerol kinase
RA 4,130,398 A→G D68G (GAC→GGC)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,132,580 T→A I389N (ATC→AAC)  katG → catalase
RA 4,139,669 +C coding (233/330 nt)
coding (132/159 nt)
yijI →
b4558 ←
hypothetical protein
hypothetical protein
RA 4,145,321 T→C A192A (GCA→GCG yijO ← putative ARAC‑type regulatory protein
RA 4,155,035 A→G T203A (ACG→GCG)  argH → argininosuccinate lyase
RA 4,158,827 G→T G61V (GGC→GTC)  yijC → hypothetical protein
RA 4,161,702 C→G A162G (GCA→GGA)  btuB → BtuB
RA 4,162,448 A→G T411A (ACA→GCA)  btuB → BtuB
RA 4,170,208 T→C G191G (GGT→GGC murB → UDP‑N‑acetylenolpyruvoylglucosamine reductase
RA 4,171,680 A→G V309A (GTA→GCA)  coaA ← pantothenate kinase
RA 4,175,949 +G intergenic (+82/‑76) nusG → / → rplK NusG/50S ribosomal subunit protein L11
RA 4,182,169 A→G H1116R (CAC→CGC)  rpoB → RNA polymerase beta subunit
RA 4,194,577 2 bp→GC coding (97‑98/774 nt) yjaD → hypothetical protein
RA 4,200,901 A→G *466W (TGA→TGG
T2A (ACG→GCG) 
hydH →
hydG →
HydH
HydG
RA 4,228,096 A→G N55S (AAC→AGC)  yjbC → hypothetical protein
RA 4,247,622 A→G T164A (ACG→GCG)  malM → MalM
RA 4,293,107 A→G T350A (ACC→GCC)  gltP → glutamate‑aspartate symport protein
RA 4,293,960 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate‑aspartate symport protein/hypothetical protein
RA 4,311,496 +C coding (301/408 nt) phnQ ← hypothetical protein
RA 4,312,334 A→G L116P (CTG→CCG)  phnP ← PhnP
RA 4,316,203 T→A Q47L (CAG→CTG)  phnK ← ATP‑binding component of phosphonate transport
RA 4,325,494 C→G A261G (GCG→GGG)  yjdA → putative vimentin
RA 4,334,019 A→G L197L (CTT→CTC yjdE ← putative amino acid/amine transport protein
RA 4,337,885 A→G V73A (GTC→GCC)  adiA ← biodegradative arginine decarboxylase
RA 4,354,595 A→G L534P (CTG→CCG)  cadA ← lysine decarboxylase 1
RA 4,362,298 T→C Q108R (CAG→CGG)  dsbD ← thiol:disulfide interchange protein
RA 4,384,043 A→G V969A (GTG→GCG)  yjeP ← putative periplasmic binding protein
RA 4,389,966 A→G noncoding (29/76 nt) glyV → tRNA‑Gly
RA 4,392,651 (G)5→4 coding (1008/1548 nt) yjeF → hypothetical protein
RA 4,395,798 A→G H270R (CAC→CGC)  mutL → MutL
RA 4,402,992 A→G N243S (AAC→AGC)  purA → adenylosuccinate synthetase
RA 4,413,828 A→G Y19H (TAT→CAT)  b4188 ← hypothetical protein
RA 4,433,048 A→G Y366H (TAT→CAT)  cpdB ← 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase
RA 4,435,684 +A coding (400/501 nt) ytfI → hypothetical protein
RA 4,444,741 A→G E1018G (GAG→GGG)  ytfN → hypothetical protein
RA 4,448,541 +C intergenic (+46/‑92) ytfQ → / → ytfR putative LACI‑type transcriptional regulator/YtfR
RA 4,448,543 +C intergenic (+48/‑90) ytfQ → / → ytfR putative LACI‑type transcriptional regulator/YtfR
RA 4,449,738 +G coding (1106/1254 nt) ytfR → YtfR
RA 4,467,145 A→G L649L (TTA→TTG mgtA → Mg2+ transport ATPase, P‑type 1
RA 4,472,408 A→G intergenic (+117/‑28) b4251 → / → b4252 hypothetical protein/hypothetical protein
RA 4,474,826 +A intergenic (+1/+44) yjgL → / ← argI hypothetical protein/ornithine carbamoyltransferase 1
MC JC 4,474,849 20 bp→30 bp intergenic (+24/+2) yjgL → / ← argI hypothetical protein/ornithine carbamoyltransferase 1
RA 4,476,406 A→G D124G (GAC→GGC)  yjgD → hypothetical protein
RA 4,477,041 Δ1 bp intergenic (‑151/‑269) b4256 ← / → yjgN hypothetical protein/hypothetical protein
RA 4,477,068 +C intergenic (‑178/‑242) b4256 ← / → yjgN hypothetical protein/hypothetical protein
RA 4,477,281 Δ1 bp intergenic (‑391/‑29) b4256 ← / → yjgN hypothetical protein/hypothetical protein
RA 4,477,321 Δ1 bp coding (12/1182 nt) yjgN → hypothetical protein
RA 4,478,503 (A)7→6 intergenic (+12/+47) yjgN → / ← valS hypothetical protein/valine tRNA synthetase
RA 4,478,511 Δ1 bp intergenic (+20/+39) yjgN → / ← valS hypothetical protein/valine tRNA synthetase
RA 4,478,527 Δ1 bp intergenic (+36/+23) yjgN → / ← valS hypothetical protein/valine tRNA synthetase
RA 4,490,864 T→C Q19R (CAG→CGG)  idnO ← 5‑keto‑D‑gluconate 5‑reductase
RA 4,502,680 C→T A352V (GCA→GTA)  b4279 → putative transport protein
RA 4,532,411 A→G intergenic (‑1059/‑172) yjhQ ← / → yjhR hypothetical protein/putative frameshift suppressor
RA 4,542,643 Δ1 bp intergenic (+46/‑22) b4316 → / → fimD Pil/FimD
RA 4,544,170 T→C I502I (ATT→ATC fimD → FimD
RA 4,555,245 T→C Y205C (TAC→TGC)  yjiE ← putative transcriptional regulator LYSR‑type
RA 4,572,272 G→A G190E (GGG→GAG)  yjiU → hypothetical protein
RA 4,573,190 Δ1 bp coding (1487/1542 nt) yjiU → hypothetical protein
RA 4,583,050 G→A Q446* (CAA→TAA)  hsdR ← endonuclease R
RA 4,593,254 2 bp→GC coding (59‑60/807 nt) yjjM ← hypothetical protein
RA 4,593,336 +G intergenic (‑23/‑208) yjjM ← / → yjjN hypothetical protein/putative oxidoreductase
RA 4,595,908 T→C Q355R (CAG→CGG)  mdoB ← phosphoglycerol transferase I
RA 4,600,078 C→T W135* (TGG→TAG)  yjjP ← putative structural protein
RA 4,601,332 A→G Q96R (CAG→CGG)  yjjQ → putative regulator
RA 4,603,663 (G)8→9 noncoding (72/88 nt) leuV ← tRNA‑Leu
RA 4,608,595 2 bp→GC intergenic (+23/‑369) prfC → / → osmY peptide chain release factor RF‑3/OsmY
RA 4,614,238 G→T L133I (CTA→ATA)  yjjI ← hypothetical protein
RA 4,618,604 T→C G51G (GGT→GGC deoD → purine‑nucleoside phosphorylase
RA 4,632,259 T→C A207A (GCA→GCG rob ← right origin‑binding protein
RA 4,636,923 T→C W393R (TGG→CGG)  creD → CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 363569 365526 1958 170 [164] [1] 425 lacZ beta‑D‑galactosidase
* * ÷ NC_000913 2518987–2519006 2519122–2519072 67–136 166 [164] [163] 166 [valU]–[valX] [valU],[valX]
* * ÷ NC_000913 3421336–3421870 3422242–3421880 11–907 165 [158] [158] 166 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3943608–3943706 3943790–3943771 66–183 167 [164] [161] 166 rrlC 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 269765 =NA (NA)373 (1.090) 139/184 0.3 98.7% coding (300/405 nt) yi91a hypothetical protein
?NC_000913 = 370983 5 (0.010)coding (536/882 nt) mhpC 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase
* ? NC_000913 = 279335NA (NA)338 (0.990) 148/184 0.1 96.6% coding (252/339 nt) b4505 hypothetical protein
?NC_000913 370982 = 12 (0.040)coding (535/882 nt) mhpC 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase
* ? NC_000913 365527 =1 (0.000)390 (1.180) 147/178 0.1 71.0% coding (3/3075 nt) lacZ beta‑D‑galactosidase
?NC_000913 4589230 = 318 (0.960)coding (4/1656 nt) tsr methyl‑accepting chemotaxis protein I
* ? NC_000913 3192559 =375 (1.090)295 (1.090)
+ATGTGTTAATGAAACCTTC
99/146 0.9 50.6% intergenic (+18/+402) b3051/b3052 putative membrane protein/putative kinase
?NC_000913 = 3192916 352 (1.030)intergenic (+375/+45) b3051/b3052 putative membrane protein/putative kinase
* ? NC_000913 3349258 =34 (0.100)372 (1.090) 150/184 0.1 91.6% coding (1403/2331 nt) arcB aerobic respiration sensor‑response protein
?NC_000913 = 3349263 NA (NA)coding (1398/2331 nt) arcB aerobic respiration sensor‑response protein