breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 429 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,0190TC72.7% 18.1 / 21.6 33I125V (ATT→GTT) nohANohA
*NC_000913118,7800AG68.2% 299.7 / 221.4 258S16S (TCA→TCGampDAmpD
*NC_0009134,606,8980TC68.2% 507.2 / 259.7 220intergenic (+6/‑85)yjjG/prfCputative phosphatase/peptide chain release factor RF‑3
*NC_000913878,0500TC67.9% 285.7 / 230.1 253V29A (GTA→GCA) yliIputative dehydrogenase
*NC_0009131,938,7230GA67.5% 386.7 / 218.2 265P292S (CCA→TCA) yebAhypothetical protein
*NC_0009131,774,6870GA66.9% 365.3 / 225.5 260M359I (ATG→ATAydiFhypothetical protein
*NC_0009134,185,1280CT66.6% 400.4 / 267.1 299A734V (GCG→GTG) rpoCRNA polymerase beta prime subunit
*NC_0009131,129,7330TC66.5% 262.5 / 223.8 242K118K (AAA→AAGflgAFlgA
*NC_0009132,533,0810TC66.2% 332.9 / 290.9 317R332R (CGT→CGCptsIPEP‑protein phosphotransferase system enzyme I
*NC_0009132,845,3200AG66.0% 239.9 / 228.3 241V39A (GTG→GCG) hycDmembrane‑spanning protein of hydrogenase 3
*NC_0009131,238,4970CT65.9% 320.2 / 236.4 265G132G (GGC→GGTdadXalanine racemase 2, catabolic
*NC_000913158,3000AG65.4% 267.2 / 302.0 295F265S (TTC→TCC) pcnBpoly(A) polymerase I
*NC_0009132,497,5950TC64.8% 262.5 / 294.9 291V302A (GTG→GCG) b2380putative sensor protein
*NC_0009134,136,5220AG64.8% 247.8 / 311.8 290W32R (TGG→CGG) b3945glycerol dehydrogenase (NAD)
*NC_0009133,236,5620TC64.6% 314.1 / inf 357V114A (GTG→GCG) ygjTputative transport protein
*NC_0009131,232,5090CT64.5% 324.5 / 260.2 288A478T (GCC→ACC) nhaBNa+/H+ antiporter, pH independent
*NC_0009133,488,6960AG64.3% 260.6 / inf 326H135H (CAT→CACficFic
*NC_000913412,1240AG64.2% 268.0 / inf 313S952P (TCC→CCC) sbcCATP‑dependent dsDNA exonuclease
*NC_000913493,3710GA64.0% 303.3 / 255.7 264M24I (ATG→ATAybaBhypothetical protein
*NC_0009132,208,0580TC64.0% 267.1 / 314.8 301G313G (GGT→GGCb2122hypothetical protein

Marginal new junction evidence (lowest skew 10 of 16 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1207028171 (0.500)139 (0.490) 81/152 3.0 53.4% coding (305/630 nt) ycfK hypothetical protein
?NC_000913 = 1208825 102 (0.360)coding (57/540 nt) b1157 putative tail fiber protein
* ? NC_000913 1207013 =180 (0.530)130 (0.460) 77/152 3.6 49.5% coding (290/630 nt) ycfK hypothetical protein
?NC_000913 1208842 = 117 (0.410)coding (40/540 nt) b1157 putative tail fiber protein
* ? NC_000913 = 4008398137 (0.410)145 (0.440)
+AAGA
32/172 14.3 52.3% coding (481/516 nt) b4555 hypothetical protein
?NC_000913 4008399 = 137 (0.400)coding (482/516 nt) b4555 hypothetical protein
* ? NC_000913 = 1208841158 (0.460)26 (0.090) 17/154 18.4 11.7% coding (41/540 nt) b1157 putative tail fiber protein
?NC_000913 2469917 = 259 (0.900)coding (166/519 nt) yfdL putative RNA polymerase beta
* ? NC_000913 = 4540258162 (0.470)11 (0.040) 9/166 23.6 5.3% intergenic (+57/‑425) fimE/fimA FimE/major type 1 subunit fimbrin
?NC_000913 = 4540554 244 (0.790)intergenic (+353/‑129) fimE/fimA FimE/major type 1 subunit fimbrin
* ? NC_000913 4540250 =177 (0.520)6 (0.020) 6/166 25.6 2.9% intergenic (+49/‑433) fimE/fimA FimE/major type 1 subunit fimbrin
?NC_000913 4540564 = 244 (0.790)intergenic (+363/‑119) fimE/fimA FimE/major type 1 subunit fimbrin
* ? NC_000913 = 679422318 (0.930)35 (0.110) 7/178 25.7 10.2% coding (692/708 nt) ybeU putative tRNA ligase
?NC_000913 687077 = NA (NA)intergenic (‑107/+143) ybeJ/trs53 putative periplasmic binding transport protein/IS5 transposase
* ? NC_000913 = 381812NA (NA)18 (0.050) 5/180 27.3 6.3% coding (303/387 nt) b0362 hypothetical protein
?NC_000913 578092 = 275 (0.800)coding (25/294 nt) ybcU Bor‑like protein
* ? NC_000913 = 578098275 (0.800)18 (0.050) 5/184 27.6 6.1% coding (19/294 nt) ybcU Bor‑like protein
?NC_000913 = 1467264 NA (NA)intergenic (‑46/‑118) yi212/tra82 hypothetical protein/IS30 transposase
* ? NC_000913 721798 =287 (0.840)3 (0.010) 3/166 28.2 1.2% coding (1840/2685 nt) kdpD KdpD
?NC_000913 = 721794 253 (0.820)coding (1844/2685 nt) kdpD KdpD