Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 817,223 T→C G319G (GGT→GGC)  moaA → molybdopterin biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913817,2230TC100.0% 812.4 / NA 231G319G (GGT→GGC) moaAmolybdopterin biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (114/117);  total (114/117)

Only 100 of 234 total aligned reads displayed.

CGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCG  >  NC_000913/817133‑817312
                                                                                          |                                                                                         
  aTACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTa                                                                                       >  1:4588686/1‑93 (MQ=255)
   tACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTAc                                                                                      >  1:2399166/1‑93 (MQ=255)
      ccAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCa                                                                                   >  1:1406751/1‑93 (MQ=255)
        aGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCaaa                                                                                 >  1:2415031/1‑93 (MQ=255)
        aGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCaaa                                                                                 >  2:5517562/1‑93 (MQ=255)
        aGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCaaa                                                                                 <  1:2591217/93‑1 (MQ=255)
         gCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCaaaa                                                                                >  2:3233008/1‑93 (MQ=255)
           aaCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACt                                                                              >  2:1142204/1‑93 (MQ=255)
           aaCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACt                                                                              <  2:4984149/93‑1 (MQ=255)
           aaCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACt                                                                              >  2:6433655/1‑93 (MQ=255)
            aCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACtt                                                                             >  1:3404871/1‑93 (MQ=255)
            aCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACtt                                                                             <  1:2740620/93‑1 (MQ=255)
             cAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTa                                                                            >  1:954567/1‑93 (MQ=255)
             cAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTa                                                                            <  1:4835552/93‑1 (MQ=255)
              aGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTAt                                                                           >  1:6221653/1‑93 (MQ=255)
                gcgcTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCg                                                                         <  2:5490147/93‑1 (MQ=255)
                  gcTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTa                                                                       >  1:1390573/1‑93 (MQ=255)
                   cTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTAc                                                                      <  2:2012850/93‑1 (MQ=255)
                    tGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACa                                                                     >  1:2690805/1‑93 (MQ=255)
                     ggAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAt                                                                    >  1:4257610/1‑93 (MQ=255)
                     ggAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAt                                                                    <  1:853353/93‑1 (MQ=255)
                     ggAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAt                                                                    <  1:676835/93‑1 (MQ=255)
                     ggAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAt                                                                    >  2:1810769/1‑93 (MQ=255)
                     ggAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAt                                                                    >  2:3109871/1‑93 (MQ=255)
                      gAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAtt                                                                   >  1:4047797/1‑93 (MQ=255)
                       aaGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTg                                                                  >  2:4234525/1‑93 (MQ=255)
                        aGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTgg                                                                 >  2:3722549/1‑93 (MQ=255)
                         gcgcGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTggc                                                                <  1:5868129/93‑1 (MQ=255)
                            cgTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACTGGCATTACGCAAAACTTATCGTACATTggcggc                                                             <  2:6305036/93‑1 (MQ=255)
                            cgTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTggcggc                                                             <  1:3292225/93‑1 (MQ=255)
                            cgTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTggcggc                                                             <  2:1973497/93‑1 (MQ=255)
                             gTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCt                                                            >  1:3825839/1‑93 (MQ=255)
                             gTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCt                                                            >  1:2559727/1‑93 (MQ=255)
                               aTTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTaa                                                          >  1:5178441/1‑93 (MQ=255)
                                 ttCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTaaaa                                                        <  1:3773286/93‑1 (MQ=255)
                                 ttCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTaaaa                                                        <  2:2711685/93‑1 (MQ=255)
                                  tCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAAc                                                       >  2:5262647/1‑93 (MQ=255)
                                   cAGCGGCGCTGCGGGAGAAGAAACAGAGCCATTTCCTGCATCAGAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACg                                                      >  1:2476812/1‑93 (MQ=255)
                                    aGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGt                                                     >  2:5402132/1‑93 (MQ=255)
                                     gcggcgCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTc                                                    <  1:1636495/93‑1 (MQ=255)
                                       ggcgCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCaa                                                  >  1:1620492/1‑93 (MQ=255)
                                         cgcTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCaaaa                                                >  2:23307/1‑93 (MQ=255)
                                          gcTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAg                                               >  2:2956936/1‑93 (MQ=255)
                                          gcTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAg                                               <  2:1102432/93‑1 (MQ=255)
                                          gcTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAg                                               <  1:1060243/93‑1 (MQ=255)
                                            tGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGga                                             <  1:1473987/93‑1 (MQ=255)
                                            tGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGga                                             <  1:2940737/93‑1 (MQ=255)
                                             gCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgag                                            >  1:5260735/1‑93 (MQ=255)
                                              cGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgaga                                           <  2:3996097/93‑1 (MQ=255)
                                              cGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgaga                                           <  1:1716127/93‑1 (MQ=255)
                                              cGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgaga                                           <  1:4777931/93‑1 (MQ=255)
                                              cGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAACgaga                                           >  1:2242784/1‑93 (MQ=255)
                                               gggAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgagag                                          >  1:4477398/1‑93 (MQ=255)
                                                 gagaAGAAACGGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGAt                                        <  1:3643554/93‑1 (MQ=255)
                                                 gagaAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGAt                                        >  1:4317154/1‑93 (MQ=255)
                                                 gagaAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGAt                                        <  1:3020741/93‑1 (MQ=255)
                                                   gaagaaACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCa                                      <  1:5509895/93‑1 (MQ=255)
                                                   gaagaaACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCa                                      <  2:5681480/93‑1 (MQ=255)
                                                   gaagaaACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCa                                      <  1:5354085/93‑1 (MQ=255)
                                                     agaaACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGa                                    <  2:3412845/93‑1 (MQ=255)
                                                     agaaACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGa                                    >  1:4175615/1‑93 (MQ=255)
                                                      gaaACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGAt                                   >  2:4728044/1‑93 (MQ=255)
                                                        aaCAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGCGATCAGATGa                                 >  2:3745575/1‑93 (MQ=255)
                                                        aaCAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGa                                 >  2:143885/1‑93 (MQ=255)
                                                         aCAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACCTTGGCGGCTAAAACGTCCAAAGGAGAGATCAGATGAg                                >  1:5431619/1‑93 (MQ=255)
                                                         aCAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAg                                >  1:2003353/1‑93 (MQ=255)
                                                          cAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGt                               >  1:4986804/1‑93 (MQ=255)
                                                            gACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCa                             <  1:390339/93‑1 (MQ=255)
                                                            gACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCa                             <  2:5872839/93‑1 (MQ=255)
                                                             aCCCATTTCATGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAg                            >  1:3075840/1‑93 (MQ=255)
                                                              cccATTTCCTGCATCAAAACAACACCGGCATTACGCAAACCTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAgg                           <  2:4576007/93‑1 (MQ=255)
                                                              cccATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAgg                           <  1:3456117/93‑1 (MQ=255)
                                                               ccATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGt                          <  2:902520/93‑1 (MQ=255)
                                                               ccATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGACAGGt                          >  2:4497948/1‑93 (MQ=255)
                                                                cATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTa                         >  1:639531/1‑93 (MQ=255)
                                                                 aTTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAATCGTCAAAAGGAGAGATCAGATGAGTCAGGTaa                        <  1:3867138/93‑1 (MQ=255)
                                                                 aTTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTaa                        >  2:3493324/1‑93 (MQ=255)
                                                                 aTTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTaa                        >  2:1152771/1‑93 (MQ=255)
                                                                  tttCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAg                       >  2:2421130/1‑93 (MQ=255)
                                                                   ttCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGc                      <  2:4219973/93‑1 (MQ=255)
                                                                       tGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTg                  <  1:6109150/93‑1 (MQ=255)
                                                                        gCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGa                 >  2:3915617/1‑93 (MQ=255)
                                                                        gCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGa                 <  2:5732398/93‑1 (MQ=255)
                                                                         cATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGaa                <  1:6061148/93‑1 (MQ=255)
                                                                         cATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGaa                <  2:6245578/93‑1 (MQ=255)
                                                                         cATCAAAACAACACCGGCATTACCCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGaa                >  2:4844616/1‑93 (MQ=255)
                                                                          aTCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAAt               >  2:4402667/1‑93 (MQ=255)
                                                                           tCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAAtt              <  1:1072645/93‑1 (MQ=255)
                                                                             aaaaCAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTa            <  1:1875081/93‑1 (MQ=255)
                                                                              aaaCAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTAt           >  1:3580449/1‑93 (MQ=255)
                                                                              aaaCAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTAt           >  1:1253643/1‑93 (MQ=255)
                                                                               aacaacACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATc          >  2:1850661/1‑93 (MQ=255)
                                                                               aacaacACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATc          >  2:5583135/1‑93 (MQ=255)
                                                                               aacaacACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATc          >  2:3399050/1‑93 (MQ=255)
                                                                                 caacaCCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATccc        <  2:4139356/93‑1 (MQ=255)
                                                                                 caacaCCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATccc        <  2:6006206/93‑1 (MQ=255)
                                                                                 caacaCCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATccc        <  1:1132845/93‑1 (MQ=255)
                                                                                   acacCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGa      >  1:2640696/1‑93 (MQ=255)
                                                                                     acCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGAcc    >  2:639413/1‑93 (MQ=255)
                                                                                      ccGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGAccc   <  2:820469/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCG  >  NC_000913/817133‑817312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTA  >  NC_000913/817130‑817314
                                                                                             |                                                                                           
AGACGATGCCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGC                                                                                             <  SRR4302534.1932372/94‑1 (MQ=60)
AGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGC                                                                                             <  SRR4302534.5286107/94‑1 (MQ=60)
 GACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCA                                                                                            >  SRR4302534.5107950/1‑94 (MQ=60)
  ACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCCGGTGAAGACACAGACCCATTTCCTGCATCAAAACAACACCGGCAT                                                                                           >  SRR4302534.633487/1‑94 (MQ=60)
   CGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATT                                                                                          >  SRR4302534.5096111/1‑94 (MQ=60)
    GATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTA                                                                                         <  SRR4302534.4890576/94‑1 (MQ=60)
    GATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTA                                                                                         >  SRR4302534.4713209/1‑94 (MQ=60)
    CATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTA                                                                                         <  SRR4302534.3858096/94‑1 (MQ=60)
     ATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTAC                                                                                        <  SRR4302534.2717455/94‑1 (MQ=60)
     ATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTAC                                                                                        >  SRR4302534.2462915/1‑94 (MQ=60)
      TACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACG                                                                                       <  SRR4302534.4367113/94‑1 (MQ=60)
        CCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCA                                                                                     >  SRR4302534.1443748/1‑94 (MQ=60)
          CAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAA                                                                                   >  SRR4302534.2479261/1‑94 (MQ=60)
           AGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAA                                                                                  <  SRR4302534.2659813/94‑1 (MQ=60)
           AGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAA                                                                                  >  SRR4302534.4515417/1‑94 (MQ=60)
              AACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTT                                                                               >  SRR4302534.3496313/1‑94 (MQ=60)
              AACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTT                                                                               >  SRR4302534.2678234/1‑94 (MQ=60)
              AACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTT                                                                               >  SRR4302534.4841526/1‑94 (MQ=60)
              AACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTT                                                                               <  SRR4302534.3990675/94‑1 (MQ=60)
               ACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTA                                                                              >  SRR4302534.2101767/1‑94 (MQ=60)
               ACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTA                                                                              <  SRR4302534.6165372/94‑1 (MQ=60)
               ACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTA                                                                              <  SRR4302534.2812839/94‑1 (MQ=60)
               ACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTA                                                                              >  SRR4302534.978871/1‑94 (MQ=60)
                CAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTAT                                                                             <  SRR4302534.4966746/94‑1 (MQ=60)
                CAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTAT                                                                             >  SRR4302534.2480464/1‑94 (MQ=60)
                CAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTAT                                                                             <  SRR4302534.1463753/94‑1 (MQ=60)
                CAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTAT                                                                             >  SRR4302534.6390630/1‑94 (MQ=60)
                   GCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGT                                                                          >  SRR4302534.3878147/1‑94 (MQ=60)
                    CGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTA                                                                         <  SRR4302534.3904198/94‑1 (MQ=60)
                    CGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTA                                                                         >  SRR4302534.1427176/1‑94 (MQ=60)
                      CTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACA                                                                       >  SRR4302534.2761912/1‑94 (MQ=60)
                       TGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAT                                                                      >  SRR4302534.2708418/1‑94 (MQ=60)
                       TGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACAT                                                                      >  SRR4302534.4372546/1‑94 (MQ=60)
                        GGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATT                                                                     <  SRR4302534.874884/94‑1 (MQ=60)
                        GGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATT                                                                     >  SRR4302534.4156354/1‑94 (MQ=60)
                        GGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATT                                                                     <  SRR4302534.3265139/94‑1 (MQ=60)
                        GGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATT                                                                     <  SRR4302534.693351/94‑1 (MQ=60)
                            GCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCG                                                                 <  SRR4302534.6027460/94‑1 (MQ=60)
                              GCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGC                                                               <  SRR4302534.6261958/94‑1 (MQ=60)
                               CGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCT                                                              <  SRR4302534.6224261/94‑1 (MQ=60)
                               CGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCT                                                              <  SRR4302534.3380014/94‑1 (MQ=60)
                               CGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCT                                                              >  SRR4302534.2627578/1‑94 (MQ=60)
                               CGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCT                                                              >  SRR4302534.3928617/1‑94 (MQ=60)
                                GTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTA                                                             >  SRR4302534.799997/1‑94 (MQ=60)
                                GTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTA                                                             <  SRR4302534.511364/94‑1 (MQ=60)
                                 TATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAA                                                            >  SRR4302534.5317964/1‑94 (MQ=60)
                                   TTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAA                                                          <  SRR4302534.1429363/94‑1 (MQ=60)
                                    TTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAAC                                                         <  SRR4302534.3874599/94‑1 (MQ=60)
                                    TTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAAC                                                         <  SRR4302534.5402774/94‑1 (MQ=60)
                                     TCAGCGGCGCTGCGGGAGAAGAAACAGAGCCATTTCCTGCATCAGAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACG                                                        >  SRR4302534.2542629/1‑94 (MQ=60)
                                     TCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACG                                                        <  SRR4302534.3738801/94‑1 (MQ=60)
                                        GCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCA                                                     <  SRR4302534.1567970/94‑1 (MQ=60)
                                        GCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCA                                                     <  SRR4302534.1679150/94‑1 (MQ=60)
                                         CGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAA                                                    >  SRR4302534.4047953/1‑94 (MQ=60)
                                         CGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAA                                                    >  SRR4302534.1662770/1‑94 (MQ=60)
                                            CGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAG                                                 <  SRR4302534.6518219/94‑1 (MQ=60)
                                            CGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAG                                                 <  SRR4302534.3499136/94‑1 (MQ=60)
                                             GCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGG                                                <  SRR4302534.1087724/94‑1 (MQ=60)
                                             GCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGG                                                <  SRR4302534.1226675/94‑1 (MQ=60)
                                               TGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAG                                              >  SRR4302534.5402874/1‑94 (MQ=60)
                                               TGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAG                                              >  SRR4302534.1669592/1‑94 (MQ=60)
                                               TGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAG                                              <  SRR4302534.4960467/94‑1 (MQ=60)
                                               TGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAG                                              <  SRR4302534.1512769/94‑1 (MQ=60)
                                               TGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAG                                              <  SRR4302534.3017901/94‑1 (MQ=60)
                                               TGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAG                                              <  SRR4302534.2765107/94‑1 (MQ=60)
                                                GCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGA                                             <  SRR4302534.1395336/94‑1 (MQ=60)
                                                GCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAACGAGA                                             >  SRR4302534.2302214/1‑94 (MQ=60)
                                                 attGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAG                                            <  SRR4302534.5540345/91‑1 (MQ=60)
                                                 CGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAG                                            >  SRR4302534.4422852/1‑94 (MQ=60)
                                                 CGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAG                                            <  SRR4302534.1760709/94‑1 (MQ=60)
                                                 CGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAG                                            >  SRR4302534.4598531/1‑94 (MQ=60)
                                                 CGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAG                                            <  SRR4302534.4907678/94‑1 (MQ=60)
                                                  GGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGA                                           >  SRR4302534.4183985/1‑94 (MQ=60)
                                                   GGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGAT                                          >  SRR4302534.4434054/1‑94 (MQ=60)
                                                   GGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGAT                                          <  SRR4302534.995760/94‑1 (MQ=60)
                                                   GGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGAT                                          <  SRR4302534.170770/94‑1 (MQ=60)
                                                    GAGAAGAAACGGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATC                                         <  SRR4302534.3741474/94‑1 (MQ=60)
                                                    GAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATC                                         <  SRR4302534.1095920/94‑1 (MQ=60)
                                                    GAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATC                                         >  SRR4302534.4132954/1‑94 (MQ=60)
                                                    GAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATC                                         <  SRR4302534.3099851/94‑1 (MQ=60)
                                                    GAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATC                                         <  SRR4302534.4640836/94‑1 (MQ=60)
                                                     AGAAGAACCAGACCCATTGCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCA                                        <  SRR4302534.1148369/94‑1 (MQ=60)
                                                     AGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCA                                        <  SRR4302534.3449213/94‑1 (MQ=60)
                                                      GAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAG                                       <  SRR4302534.5658528/94‑1 (MQ=60)
                                                      GAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAG                                       <  SRR4302534.5498430/94‑1 (MQ=60)
                                                       AAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGA                                      >  SRR4302534.4288403/1‑94 (MQ=60)
                                                        AGAAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGAT                                     >  SRR4302534.2469856/1‑94 (MQ=60)
                                                          AAACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGACGA                                   <  SRR4302534.6345446/94‑1 (MQ=60)
                                                           AACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACCTTGGCGGCTAAAACGTCCAAAGGAGAGATCAGATGAG                                  >  SRR4302534.5577842/1‑94 (MQ=60)
                                                           AACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAG                                  >  SRR4302534.2055794/1‑94 (MQ=60)
                                                            ACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGT                                 >  SRR4302534.3946616/1‑94 (MQ=60)
                                                            ACAGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGT                                 >  SRR4302534.5121746/1‑94 (MQ=60)
                                                              AGACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCA                               <  SRR4302534.3563701/94‑1 (MQ=60)
                                                               GACCCATTTCTTGCATCAAAACGACACCATCATTACGCAAAACTTATCGTACATTTGCGGCTAAAACTTCAAAAGGAGAGATCAGATGAGTCAG                              <  SRR4302534.2584666/94‑1 (MQ=60)
                                                               GACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAG                              <  SRR4302534.400315/94‑1 (MQ=60)
                                                               GACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAG                              <  SRR4302534.874033/94‑1 (MQ=60)
                                                               GACCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAG                              >  SRR4302534.170870/1‑94 (MQ=60)
                                                               GACCCATTTCATGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAG                              >  SRR4302534.3156640/1‑94 (MQ=60)
                                                                 CCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGT                            <  SRR4302534.3549022/94‑1 (MQ=60)
                                                                 CCCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGT                            <  SRR4302534.6603105/94‑1 (MQ=60)
                                                                  CCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTA                           >  SRR4302534.551120/1‑94 (MQ=60)
                                                                  CCATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTA                           >  SRR4302534.655044/1‑94 (MQ=60)
                                                                    ATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAATCGTCAAAAGGAGAGATCAGATGAGTCAGGTAAG                         <  SRR4302534.3970962/94‑1 (MQ=60)
                                                                    ATTTCCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAG                         >  SRR4302534.195938/1‑94 (MQ=60)
                                                                        CCTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACT                     <  SRR4302534.2628548/94‑1 (MQ=60)
                                                                         CTGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTG                    <  SRR4302534.2238724/94‑1 (MQ=60)
                                                                          TGCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGA                   <  SRR4302534.6275291/94‑1 (MQ=60)
                                                                           GCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAA                  <  SRR4302534.5636054/94‑1 (MQ=60)
                                                                           GCATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAA                  >  SRR4302534.1497956/1‑94 (MQ=60)
                                                                            CATCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAAT                 <  SRR4302534.6225958/94‑1 (MQ=60)
                                                                              TCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTT               <  SRR4302534.1100476/94‑1 (MQ=60)
                                                                              TCAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTT               <  SRR4302534.5701702/94‑1 (MQ=60)
                                                                               CAAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTA              >  SRR4302534.932357/1‑94 (MQ=60)
                                                                                AAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTAT             >  SRR4302534.2403589/1‑94 (MQ=60)
                                                                                AAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTAT             <  SRR4302534.1923882/94‑1 (MQ=60)
                                                                                AAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTAT             >  SRR4302534.1286685/1‑94 (MQ=60)
                                                                                AAAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTAT             >  SRR4302534.3676752/1‑94 (MQ=60)
                                                                                 AAACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATC            >  SRR4302534.3334130/1‑94 (MQ=60)
                                                                                   ACAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCC          <  SRR4302534.5491304/94‑1 (MQ=60)
                                                                                   ACAACACAGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCC          <  SRR4302534.1239108/94‑1 (MQ=60)
                                                                                    CAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCG         >  SRR4302534.2964162/1‑94 (MQ=60)
                                                                                    CAACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCG         <  SRR4302534.1162462/94‑1 (MQ=60)
                                                                                     AACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGA        >  SRR4302534.2710533/1‑94 (MQ=60)
                                                                                     AACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGGCAGGTAAGCACTGAATTTATCCCCA        >  SRR4302534.2541356/1‑94 (MQ=60)
                                                                                      ACACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGAC       <  SRR4302534.3086180/94‑1 (MQ=60)
                                                                                       CACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACC      >  SRR4302534.5191256/1‑94 (MQ=60)
                                                                                       CACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGGCAGGTAAGCACTGAATTTATCCCGACC      >  SRR4302534.4381466/1‑94 (MQ=60)
                                                                                        ACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCC     >  SRR4302534.2953879/1‑94 (MQ=60)
                                                                                        ACCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCC     >  SRR4302534.31104/1‑94 (MQ=60)
                                                                                         CCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCG    <  SRR4302534.3587663/94‑1 (MQ=60)
                                                                                         CCGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCG    <  SRR4302534.2575746/94‑1 (MQ=60)
                                                                                          CGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGT   <  SRR4302534.1831116/94‑1 (MQ=60)
                                                                                          CGGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGT   >  SRR4302534.4215767/1‑94 (MQ=60)
                                                                                           GGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTA  >  SRR4302534.4117188/1‑94 (MQ=60)
                                                                                           GGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTA  >  SRR4302534.5645948/1‑94 (MQ=60)
                                                                                           GGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTA  >  SRR4302534.200439/1‑94 (MQ=60)
                                                                                           GGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTA  >  SRR4302534.4692922/1‑94 (MQ=60)
                                                                                           GGCATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTA  >  SRR4302534.4931692/1‑94 (MQ=60)
                                                                                             |                                                                                           
AGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTA  >  NC_000913/817130‑817314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑