Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	866,582	A→G	V264V (GTA→GTG)	ybiK →	putative asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	866,582	0	A	G	100.0%	665.1 / NA	192	V264V (GTA→GTG)	ybiK	putative asparaginase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (92/100); total (92/100)

Only 100 of 217 total aligned reads displayed.

GTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGG  >  NC_000913/866493‑866671

  cTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGCACCCTGCGACCGGGGAGTGATGGa                                                                                      >  2:2805686/1‑93 (MQ=255)

  cTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGa                                                                                      >  1:357163/1‑93 (MQ=255)

      aTCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGaaaaa                                                                                  >  2:1048197/1‑93 (MQ=255)

       tCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAAc                                                                                 <  2:594366/93‑1 (MQ=255)

       tCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAAc                                                                                 >  2:1300133/1‑93 (MQ=255)

         cgcgcgCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTc                                                                               >  2:5745669/1‑93 (MQ=255)

         cgcgcgCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTc                                                                               <  1:3734491/93‑1 (MQ=255)

         cgcgcgCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGGGATGGGAAAAATc                                                                               >  1:4402841/1‑93 (MQ=255)

          gcgcgcTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTcc                                                                              >  2:3914818/1‑93 (MQ=255)

           cgcgcTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTccc                                                                             <  1:5417979/93‑1 (MQ=255)

           cgcgcTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTccc                                                                             >  1:5742862/1‑93 (MQ=255)

            gcgcTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCt                                                                            >  1:5014249/1‑93 (MQ=255)

            gcgcTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCt                                                                            <  1:2782767/93‑1 (MQ=255)

              gcTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTgc                                                                          <  1:1969692/93‑1 (MQ=255)

                  gcggcATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCtt                                                                      >  2:815767/1‑93 (MQ=255)

                     gcATATGACATCGCGGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGc                                                                   <  1:3194146/93‑1 (MQ=255)

                      cATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCg                                                                  >  1:2565254/1‑93 (MQ=255)

                        tatGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGaaa                                                                                    >  1:1481191/1‑73 (MQ=255)

                        tatGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGaaa                                                                                    <  2:1481191/73‑1 (MQ=255)

                        tatGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGt                                                                <  2:5407617/93‑1 (MQ=255)

                          tGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAg                                                              <  1:3215865/93‑1 (MQ=255)

                          tGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGGAg                                                              >  1:4597321/1‑93 (MQ=255)

                           gACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGc                                                             <  1:1867059/93‑1 (MQ=255)

                           gACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGc                                                             <  1:1386485/93‑1 (MQ=255)

                             cATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCgg                                                           >  1:3924283/1‑93 (MQ=255)

                              aTCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCggg                                                          >  2:5606146/1‑92 (MQ=255)

                              aTCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGGAGCggg                                                          >  1:4568249/1‑92 (MQ=255)

                              aTCGCCGCCGTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGt                                                          <  1:988664/93‑1 (MQ=255)

                               tCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCgggg                                                         >  1:2740219/1‑91 (MQ=255)

                               tCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTg                                                         <  1:2340885/93‑1 (MQ=255)

                               tCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGGGATGGAAAAACTCCCTGCGCTTGGCGGGAGCGGTg                                                         >  2:192334/1‑93 (MQ=255)

                                 gccgcGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGCGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGc                                                       <  2:3448122/93‑1 (MQ=255)

                                  ccgcGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCt                                                      <  2:2570394/93‑1 (MQ=255)

                                  ccgcGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCt                                                      <  2:4982417/93‑1 (MQ=255)

                                   cgcgTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCtt                                                     <  2:314242/93‑1 (MQ=255)

                                    gcgTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGGGATGGAAAAACTCCCTGCGCTTGGCGGGAGCGGTgggcta                                                    >  1:3477839/1‑89 (MQ=255)

                                     cgTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTaa                                                   >  2:707472/1‑93 (MQ=255)

                                      gTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAAt                                                  <  2:5121341/93‑1 (MQ=255)

                                      gTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGACGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAAt                                                  <  1:2329721/93‑1 (MQ=255)

                                       ttAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATc                                                 >  2:5843534/1‑93 (MQ=255)

                                       ttAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATc                                                 >  2:6090464/1‑93 (MQ=255)

                                         aaTGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGc                                               <  2:52995/93‑1 (MQ=255)

                                         aaTGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGc                                               <  2:6281831/93‑1 (MQ=255)

                                         aaTGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGc                                               <  2:2144938/93‑1 (MQ=255)

                                         aaTGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGc                                               <  1:2417752/93‑1 (MQ=255)

                                          aTGGATTACGGCGGATTAAGTCTCGCGGAAGGCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCt                                              >  1:5307717/1‑93 (MQ=255)

                                          aTGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCt                                              >  2:5973289/1‑93 (MQ=255)

                                          aTGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCt                                              >  1:2843349/1‑93 (MQ=255)

                                            ggATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGGTTAATCGCTAt                                            >  1:5763038/1‑93 (MQ=255)

                                            ggATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTAt                                            <  1:3005678/93‑1 (MQ=255)

                                              aTTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCg                                          <  2:5483695/93‑1 (MQ=255)

                                              aTTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCg                                          <  2:1680455/93‑1 (MQ=255)

                                               ttACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGa                                         >  1:1336750/1‑93 (MQ=255)

                                                 aCGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGAcc                                       <  1:4963521/93‑1 (MQ=255)

                                                  cggcggATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCa                                      <  1:4315467/93‑1 (MQ=255)

                                                  cggcggATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCa                                      >  2:1885899/1‑93 (MQ=255)

                                                     cggATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGa                                   >  1:6037229/1‑93 (MQ=255)

                                                      ggATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGaa                                  >  1:5510432/1‑93 (MQ=255)

                                                      ggATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGaa                                  <  2:4293257/93‑1 (MQ=255)

                                                      ggATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATAGCTATCGACCAAGaa                                  >  2:615392/1‑93 (MQ=255)

                                                       gATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAg                                 >  2:4647566/1‑93 (MQ=255)

                                                       gATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAg                                 <  2:453421/93‑1 (MQ=255)

                                                       gATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAg                                 >  1:265556/1‑93 (MQ=255)

                                                         ttAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAggg                               <  2:1548436/93‑1 (MQ=255)

                                                          tAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGa                              <  2:3413052/93‑1 (MQ=255)

                                                          tAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGa                              <  2:2317785/93‑1 (MQ=255)

                                                           aaGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGaa                             <  1:2041516/93‑1 (MQ=255)

                                                            aGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAAt                            <  1:6300063/93‑1 (MQ=255)

                                                               ctcGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTc                         <  1:2614178/93‑1 (MQ=255)

                                                                  gcgGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTcgcg                      >  2:3458740/1‑93 (MQ=255)

                                                                  gcgGAAGCCTGCGAGCGGGCAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTcgcg                      >  1:6305903/1‑93 (MQ=255)

                                                                    ggAAGCCTGCGAGCGGGTAGTGATGGAAAACCTCCCTGCGCTTGGCGCTAGCGGTGGCTTAATCGCTATCGCCCATGAAGGGAATGTCGCGCt                    <  1:3369583/93‑1 (MQ=255)

                                                                    ggAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCt                    >  2:4511135/1‑93 (MQ=255)

                                                                    ggAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCt                    <  1:6442148/93‑1 (MQ=255)

                                                                    ggAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCt                    >  2:3705671/1‑93 (MQ=255)

                                                                     gAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTa                   >  1:627114/1‑93 (MQ=255)

                                                                     gAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTa                   >  1:4798871/1‑93 (MQ=255)

                                                                       atccTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTAcc                 >  1:5117338/3‑93 (MQ=255)

                                                                       aGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTAcc                 >  1:3125207/1‑93 (MQ=255)

                                                                        gCCTGCGAGCGGGTAGTGATGGAAACACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCg                <  2:3259492/93‑1 (MQ=255)

                                                                        gCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCg                >  2:4089115/1‑93 (MQ=255)

                                                                        gCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCg                >  2:272480/1‑93 (MQ=255)

                                                                         ccggCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGt               <  2:1604013/90‑1 (MQ=255)

                                                                          cTGCGAGCGGGTAGTGATGAAAAACCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGtt              <  1:2572475/93‑1 (MQ=255)

                                                                           tGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGttt             <  2:1128103/93‑1 (MQ=255)

                                                                           tGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGttt             <  2:1950588/93‑1 (MQ=255)

                                                                           tGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGttt             >  2:2714535/1‑93 (MQ=255)

                                                                            gCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTa            >  2:6422926/1‑93 (MQ=255)

                                                                             cGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTaa           >  1:1038573/1‑93 (MQ=255)

                                                                              gAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAac          >  1:5391949/1‑93 (MQ=255)

                                                                              gAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAac          >  2:3146004/1‑93 (MQ=255)

                                                                              gAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAac          <  1:6390701/93‑1 (MQ=255)

                                                                               aGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAacc         >  1:1092447/1‑92 (MQ=255)

                                                                               aGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAaca         <  2:6283750/93‑1 (MQ=255)

                                                                               aGCGGGGAGGGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAaca         >  1:829577/1‑93 (MQ=255)

                                                                                gCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAacac        <  2:1147092/93‑1 (MQ=255)

                                                                                  gggTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCg      >  2:5885778/1‑93 (MQ=255)

                                                                                  gggTAATGAAGGAAAAACTCCCTGCGCTTGGCGGTAGGGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCg      <  1:1085203/93‑1 (MQ=255)

                                                                                    gtagtgATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGaa    <  2:386869/93‑1 (MQ=255)

                                                                                    gtagtgATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGaa    <  2:1836883/93‑1 (MQ=255)

GTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGG  >  NC_000913/866493‑866671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGAAGTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATG  >  NC_000913/866489‑866675

CGAAGTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTG                                                                                               >  SRR4302534.4017719/1‑94 (MQ=60)

 GAAGTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGA                                                                                              <  SRR4302534.4393900/94‑1 (MQ=60)

 GAAGTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGA                                                                                              >  SRR4302534.5209408/1‑94 (MQ=60)

 GAAGTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGA                                                                                              >  SRR4302534.6193964/1‑94 (MQ=60)

   AGTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATG                                                                                            >  SRR4302534.6523909/1‑94 (MQ=60)

     TCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGA                                                                                          >  SRR4302534.5560308/1‑94 (MQ=60)

     TCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGA                                                                                          >  SRR4302534.366296/1‑94 (MQ=60)

           TCGGCGCGCTGGCGGCATATGAGATCGATACGGTAATGGATTCCGGCGGATTAAGTCTCGCAGGAGTCTGCGAGCGGGTAGTGATGGAAAAACT                                                                                    <  SRR4302534.4312900/94‑1 (MQ=36)

            CCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTC                                                                                   >  SRR4302534.4473803/1‑94 (MQ=60)

             CGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCC                                                                                  <  SRR4302534.3834813/94‑1 (MQ=60)

              GCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCC                                                                                 <  SRR4302534.267381/94‑1 (MQ=60)

              GCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCC                                                                                 <  SRR4302534.288061/94‑1 (MQ=60)

              GCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCC                                                                                 >  SRR4302534.5898550/1‑94 (MQ=60)

               CGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCT                                                                                >  SRR4302534.5149761/1‑94 (MQ=60)

               CGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCT                                                                                <  SRR4302534.5563746/94‑1 (MQ=60)

               CGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCACT                                                                                >  SRR4302534.3220670/1‑94 (MQ=60)

                GCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTG                                                                               <  SRR4302534.2855952/94‑1 (MQ=60)

                GCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTG                                                                               >  SRR4302534.2857504/1‑94 (MQ=60)

                  GCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCG                                                                             <  SRR4302534.2021253/94‑1 (MQ=60)

                  GCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGGAAAACTCCCTGCG                                                                             >  SRR4302534.1386993/1‑94 (MQ=60)

                     GGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTT                                                                          <  SRR4302534.43782/94‑1 (MQ=60)

                       CGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGG                                                                        <  SRR4302534.4686759/94‑1 (MQ=60)

                         GCATATGACATCGCGGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCG                                                                      <  SRR4302534.3278795/94‑1 (MQ=60)

                         GCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCG                                                                      <  SRR4302534.3156967/94‑1 (MQ=60)

                         GCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCG                                                                      >  SRR4302534.2633238/1‑94 (MQ=60)

                         GCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCG                                                                      <  SRR4302534.3290722/94‑1 (MQ=60)

                          CATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCCGGAGCCTGCGAGCCGGTAGGGATGGAAAAAATCCCCGCGCTTGGCGG                                                                     >  SRR4302534.41347/1‑94 (MQ=60)

                           ATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAAatctatagatcggaagag                                                                    >  SRR4302534.1520146/1‑76 (MQ=60)

                           ATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCCTGGCGGT                                                                    >  SRR4302534.2639901/1‑94 (MQ=60)

                            TATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAACCTCCCTGCGCTTGGCGGTA                                                                   <  SRR4302534.5735885/94‑1 (MQ=60)

                            TATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTA                                                                   <  SRR4302534.2586327/94‑1 (MQ=60)

                             ATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAG                                                                  <  SRR4302534.6253663/94‑1 (MQ=60)

                             ATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGGAG                                                                  >  SRR4302534.4722089/1‑94 (MQ=60)

                              TGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGC                                                                 <  SRR4302534.3301215/94‑1 (MQ=60)

                               GACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCG                                                                <  SRR4302534.1422976/94‑1 (MQ=60)

                               GACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCG                                                                <  SRR4302534.1915647/94‑1 (MQ=60)

                               GACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCG                                                                >  SRR4302534.1075558/1‑94 (MQ=60)

                                ACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGG                                                               >  SRR4302534.4029416/1‑94 (MQ=60)

                                ACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGG                                                               >  SRR4302534.1941376/1‑94 (MQ=60)

                                 CATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGGAGCGGG                                                              >  SRR4302534.4692101/1‑94 (MQ=60)

                                  ATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGGG                                                             >  SRR4302534.2812421/1‑94 (MQ=60)

                                  ATCGCCGCCGTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTG                                                             <  SRR4302534.1013948/94‑1 (MQ=60)

                                   TCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGG                                                            <  SRR4302534.2403005/94‑1 (MQ=60)

                                   TCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGG                                                            <  SRR4302534.1680611/94‑1 (MQ=60)

                                    CGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGC                                                           >  SRR4302534.3994771/1‑94 (MQ=60)

                                    CGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGGAAAACTCCCCGCGCTTGGCGGGAGCGGTGGC                                                           >  SRR4302534.5217935/1‑94 (MQ=60)

                                    CGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGC                                                           >  SRR4302534.4684120/1‑94 (MQ=60)

                                       CGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGGGATGGAAAAACTCCCTGCGCTTGGCGGGAGCGGTGGgcta                                                        >  SRR4302534.3571320/1‑90 (MQ=60)

                                       CGCGTTAATGGATTACGGCGGATTAAGTCTCCCGGAAACCTGCGGGCGGGTAGTGATGGAAAAACTCCCTGCGCTTTGCGGCAGCGGTGGCTTA                                                        >  SRR4302534.5409948/1‑94 (MQ=60)

                                          GTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTAGCGGTAGCGGTGGCTTAATC                                                     <  SRR4302534.6471647/94‑1 (MQ=60)

                                          GTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGACGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATC                                                     <  SRR4302534.2391532/94‑1 (MQ=60)

                                           TTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCG                                                    >  SRR4302534.1474518/1‑94 (MQ=60)

                                            TAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGC                                                   >  SRR4302534.5911574/1‑94 (MQ=60)

                                            TAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGC                                                   >  SRR4302534.3649650/1‑94 (MQ=60)

                                             AATGGATTACGGCGGATTAAGTCTCGCGGAAGGCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCT                                                  >  SRR4302534.5450927/1‑94 (MQ=60)

                                             AATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCT                                                  <  SRR4302534.2482052/94‑1 (MQ=60)

                                             AATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCT                                                  >  SRR4302534.2918054/1‑94 (MQ=60)

                                              ATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTA                                                 <  SRR4302534.6595101/94‑1 (MQ=60)

                                               TGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGGTTAATCGCTAT                                                >  SRR4302534.5919380/1‑94 (MQ=60)

                                                GGATTACGGGGGAGTAAGTCCCGCGGAAGCCTGCGAGCGGGTGGTGATGGAAAAACTCCCTGCGCTTGGGGGTAGggcaggcctaatcagtatc                                               >  SRR4302534.87729/1‑75 (MQ=54)

                                                GGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATC                                               <  SRR4302534.3084306/94‑1 (MQ=60)

                                                  ATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGA                                             >  SRR4302534.1371991/1‑94 (MQ=60)

                                                    TACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACC                                           >  SRR4302534.1116678/1‑94 (MQ=60)

                                                     ACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCA                                          <  SRR4302534.5097898/94‑1 (MQ=60)

                                                      CGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCAT                                         <  SRR4302534.4432338/94‑1 (MQ=60)

                                                      CGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCAT                                         <  SRR4302534.6051692/94‑1 (MQ=60)

                                                      CGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCCGGGAGTGGTGGAAAAACTACCTACGCTTTGCGGTAGCGGTGGCTTAATCCCTATAGACCAA                                         >  SRR4302534.263577/1‑94 (MQ=50)

                                                        GCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGA                                       >  SRR4302534.6201302/1‑94 (MQ=60)

                                                         TGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAA                                      >  SRR4302534.6621792/1‑94 (MQ=60)

                                                         TGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAA                                      >  SRR4302534.5659080/1‑94 (MQ=60)

                                                          GGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAG                                     <  SRR4302534.6459455/94‑1 (MQ=60)

                                                          GGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAG                                     >  SRR4302534.6040874/1‑94 (MQ=60)

                                                          GGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAG                                     >  SRR4302534.5404222/1‑94 (MQ=60)

                                                          GGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAG                                     >  SRR4302534.1370973/1‑94 (MQ=60)

                                                          GGATTAAGTCTCGCGGAAGCCTGCGAGCGGGGAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAG                                     >  SRR4302534.272447/1‑94 (MQ=60)

                                                           GATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGG                                    >  SRR4302534.3349344/1‑94 (MQ=60)

                                                            ATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTGCCAGCGCTTGGCGGTAGAGGTGGCTTAATCGCTATCGACCATGTAGGG                                   >  SRR4302534.1215907/1‑94 (MQ=60)

                                                             TTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGA                                  <  SRR4302534.5118337/94‑1 (MQ=60)

                                                             TTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGA                                  >  SRR4302534.4963468/1‑94 (MQ=60)

                                                              TAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAA                                 >  SRR4302534.2762364/1‑94 (MQ=60)

                                                               AAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAAT                                <  SRR4302534.2094879/94‑1 (MQ=60)

                                                               AAGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAAT                                >  SRR4302534.348908/1‑94 (MQ=60)

                                                                AGTCTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATG                               <  SRR4302534.6470441/94‑1 (MQ=60)

                                                                   CTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCG                            <  SRR4302534.2708583/94‑1 (MQ=60)

                                                                   CTCGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCA                            <  SRR4302534.2683358/94‑1 (MQ=60)

                                                                     CGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCG                          >  SRR4302534.5448766/1‑94 (MQ=60)

                                                                     CGCGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCG                          >  SRR4302534.3515063/1‑94 (MQ=60)

                                                                     CGCGGAAGCCTGCGAGCGGGCAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCG                          >  SRR4302534.6476420/1‑94 (MQ=60)

                                                                       CGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCT                        >  SRR4302534.4706775/1‑94 (MQ=60)

                                                                       CGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCT                        <  SRR4302534.5465052/94‑1 (MQ=60)

                                                                       CGGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCT                        >  SRR4302534.2488000/1‑94 (MQ=60)

                                                                       CAGAAGCCTGCGAGCGGGTAGTGATGGAAAACCGTCTTGCGCTTGGCGGTAGCGGTTGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCT                        <  SRR4302534.2980380/94‑1 (MQ=60)

                                                                        GGAAGCCTGCGAGCGGGTAGTGATGGAAAACCTCCCTGCGCTTGGCGCTAGCGGTGGCTTAATCGCTATCGCCCATGAAGGGAATGTCGCGCTA                       <  SRR4302534.3459860/94‑1 (MQ=60)

                                                                        GGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTA                       >  SRR4302534.642320/1‑94 (MQ=60)

                                                                        GGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTA                       >  SRR4302534.4929178/1‑94 (MQ=60)

                                                                        GGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTA                       <  SRR4302534.6615759/94‑1 (MQ=60)

                                                                        GGAAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTA                       >  SRR4302534.819095/1‑94 (MQ=60)

                                                                          AATCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACC                     >  SRR4302534.5255463/1‑94 (MQ=60)

                                                                          AAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACC                     >  SRR4302534.913994/1‑94 (MQ=60)

                                                                          AAGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACC                     >  SRR4302534.3207368/1‑94 (MQ=60)

                                                                           AGCCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCG                    >  SRR4302534.1783618/1‑94 (MQ=60)

                                                                             CCTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTT                  >  SRR4302534.30322/1‑94 (MQ=60)

                                                                              CTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTT                 <  SRR4302534.2043246/94‑1 (MQ=60)

                                                                              CTGCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGAGCTACCGTTT                 >  SRR4302534.2399638/1‑94 (MQ=60)

                                                                              CTGCGAGCGGGTAGTGATGAAAAACCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTT                 <  SRR4302534.2640641/94‑1 (MQ=60)

                                                                                GCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAA               >  SRR4302534.1065376/1‑94 (MQ=60)

                                                                                GCGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAA               >  SRR4302534.2174146/1‑94 (MQ=60)

                                                                                 CGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAAC              >  SRR4302534.5537145/1‑94 (MQ=60)

                                                                                  GAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACC             >  SRR4302534.1120867/1‑94 (MQ=60)

                                                                                  GAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACA             <  SRR4302534.6563315/94‑1 (MQ=60)

                                                                                  GAGCGGGGAGGGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACA             >  SRR4302534.850453/1‑94 (MQ=60)

                                                                                    GCGGGTAGTGATGGAAAATCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACC           <  SRR4302534.2843670/94‑1 (MQ=60)

                                                                                     CGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCG          >  SRR4302534.5612789/1‑94 (MQ=60)

                                                                                      GGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGA         >  SRR4302534.243518/1‑94 (MQ=60)

                                                                                         TAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGG      >  SRR4302534.4915296/1‑94 (MQ=60)

                                                                                         TAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGG      >  SRR4302534.3789917/1‑94 (MQ=60)

                                                                                          AGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGA     >  SRR4302534.2834357/1‑94 (MQ=60)

                                                                                           GTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAA    >  SRR4302534.5788456/1‑94 (MQ=60)

                                                                                            TGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAAT   <  SRR4302534.5878151/94‑1 (MQ=60)

                                                                                            TGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAAT   <  SRR4302534.4360427/94‑1 (MQ=60)

                                                                                             GATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATG  <  SRR4302534.6010838/94‑1 (MQ=60)

CGAAGTCTTCATCCGCGCGCTGGCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATG  >  NC_000913/866489‑866675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`