Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 867,637 G→C A288P (GCC→CCC)  b0829 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913867,6370GC96.4% 634.8 / 11.2 194A288P (GCC→CCC) b0829hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (6/1);  new base C (104/83);  total (110/84)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

Only 100 of 211 total aligned reads displayed.

TTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑GCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGT  >  NC_000913/867547‑867726
                                                                                           |                                                                                         
  aGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑Ccccc                                                                                       <  2:1280471/93‑1 (MQ=255)
  aGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑Ccccc                                                                                       >  1:3497063/1‑93 (MQ=255)
  aGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑Ccccc                                                                                       >  1:3476203/1‑93 (MQ=255)
   gctgctGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑cccccc                                                                                      >  2:2574902/1‑93 (MQ=255)
     tgctgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑cccccccc                                                                                    <  1:773616/93‑1 (MQ=255)
      gctgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑cccccccca                                                                                   >  1:5493948/1‑92 (MQ=255)
      gctgTTCCCCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑Ccccccccc                                                                                   <  2:2262207/93‑1 (MQ=255)
         gTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACTG‑CCCCCCCCCATc                                                                                <  2:4084324/93‑1 (MQ=255)
         gTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATc                                                                                >  2:3832924/1‑93 (MQ=255)
           tCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGa                                                                              <  1:1522028/93‑1 (MQ=255)
             cGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGc                                                                            >  1:1035390/1‑93 (MQ=255)
             cGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGc                                                                            >  2:4695423/1‑93 (MQ=255)
               cAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCCTCGAGCaa                                                                          >  1:3128112/1‑92 (MQ=255)
                 aCTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGaa                                                                        >  2:6019999/1‑93 (MQ=255)
                 aCTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACA‑‑GCCCCCCCCATCGAGCAGaaa                                                                       >  2:544150/1‑93 (MQ=255)
                  cTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACA‑‑GCCCCCCCCATCGAGCAGaaaa                                                                      >  2:863276/1‑93 (MQ=255)
                   ttGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGaaaa                                                                      >  1:4618706/1‑93 (MQ=255)
                      gTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCCTCGAGCAGAAAACgg                                                                   >  1:82226/1‑93 (MQ=255)
                      gTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACgg                                                                   >  1:6245066/1‑93 (MQ=255)
                      gTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACgg                                                                   >  2:6072753/1‑93 (MQ=255)
                       ggCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACggt                                                                  <  1:1158465/92‑1 (MQ=255)
                        gCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACggtg                                                                 >  2:1810289/1‑93 (MQ=255)
                        gCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACggtg                                                                 <  2:6224546/93‑1 (MQ=255)
                          gATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACggtggt                                                               <  2:64076/93‑1 (MQ=255)
                          gATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACggtggg                                                               >  1:6406264/1‑92 (MQ=255)
                          gATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACggtggg                                                               >  1:13250/1‑92 (MQ=255)
                           aTGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGgtggtg                                                              >  2:5043838/1‑93 (MQ=255)
                             gAAAGGGTTAGATTATCCCCGACTTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGa                                                            <  2:635257/93‑1 (MQ=255)
                             gAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGa                                                            >  1:19057/1‑93 (MQ=255)
                             gAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGa                                                            >  2:5254449/1‑93 (MQ=255)
                              aaaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGCCCAGAAAACGGtggaggcg                                                           >  1:3871794/1‑91 (MQ=255)
                              aaaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGag                                                           >  2:1400837/1‑92 (MQ=255)
                              aaaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGAt                                                           >  2:6030471/1‑93 (MQ=255)
                               aaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATg                                                          >  2:3876474/1‑93 (MQ=255)
                               aaGGGTAAGATTATCCCCGACCTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGGAGAAAACGGTGGTGGATg                                                          <  1:5971154/93‑1 (MQ=255)
                                   gTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGCGCAGAAAACGGTGGTGGATGGCGa                                                      >  1:1083404/1‑93 (MQ=255)
                                      aGATTATCCCCGCCGTTTCCCGTTGATATCGCGTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                   <  1:4436087/93‑1 (MQ=255)
                                       gATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCt                                                  >  2:356638/1‑93 (MQ=255)
                                       gATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCt                                                  <  1:721448/93‑1 (MQ=255)
                                         ttATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                <  1:3418558/93‑1 (MQ=255)
                                         ttATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                <  2:5565445/93‑1 (MQ=255)
                                          tATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGtt                                               <  2:1665118/93‑1 (MQ=255)
                                              cccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAc                                           >  2:3652624/1‑93 (MQ=255)
                                               ccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAACTGTTTTACg                                          >  1:5568812/1‑93 (MQ=255)
                                                 gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGCCCCCCCCCCATCGAGCAGAAAACGGGGGGGGGGGGGGAACCTGTTTTACGa                                         >  1:3595406/1‑93 (MQ=255)
                                                 gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAg                                        >  2:1983645/1‑93 (MQ=255)
                                                  ccGTTTCCCGTTGATATCGCTTGAACATCCAGCCAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                                       <  2:2056962/92‑1 (MQ=255)
                                                  aCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                                       <  1:3252086/93‑1 (MQ=255)
                                                  aCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                                       <  2:1352877/93‑1 (MQ=255)
                                                    gTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGc                                     >  1:3305452/1‑93 (MQ=255)
                                                    gTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGc                                     <  2:4854280/93‑1 (MQ=255)
                                                    gTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGc                                     <  2:111341/93‑1 (MQ=255)
                                                     tttCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCg                                    >  1:2307963/1‑93 (MQ=255)
                                                       tCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                  >  1:1014282/1‑93 (MQ=255)
                                                       tCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                  >  1:2363224/1‑93 (MQ=255)
                                                       tCCCGTTGATATCGCTTGAACATCCAGCGAAACA‑‑GCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTaa                                 >  2:4332680/1‑93 (MQ=255)
                                                         ccGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAAt                                <  1:2084029/93‑1 (MQ=255)
                                                         ccGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAAt                                <  1:1800759/93‑1 (MQ=255)
                                                          catcgATATCTCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATc                               <  1:5353129/89‑1 (MQ=255)
                                                          cGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATc                               <  2:2774489/93‑1 (MQ=255)
                                                           gTTGATATCGCTTGACCATCCAGCGAAACAC‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCt                              <  1:3709041/93‑1 (MQ=255)
                                                            ttGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCtt                             <  1:2030435/93‑1 (MQ=255)
                                                            ttGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCtt                             <  1:4259101/93‑1 (MQ=255)
                                                            ttGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGAGGATGGGGGACCTGTTTTACGAGTGTGTCATCtt                             >  1:4658809/1‑93 (MQ=255)
                                                              gATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt                           >  1:635195/1‑93 (MQ=255)
                                                              gATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt                           >  2:2952467/1‑93 (MQ=255)
                                                              gATATCGCTTGAACATCCAACGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt                           >  1:197010/1‑93 (MQ=255)
                                                               atctCGCTGGAACATGCAGGGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCATAATCTTGTc                          <  1:5028649/90‑1 (MQ=255)
                                                               atatCGCTTGACCATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTc                          <  2:5008465/93‑1 (MQ=255)
                                                                tatCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGtta                         >  1:2403563/1‑91 (MQ=255)
                                                                tatCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCa                         <  1:5111255/93‑1 (MQ=255)
                                                                  tCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAcc                       <  2:4771790/93‑1 (MQ=255)
                                                                  tCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGAGGATGGCGAACCTGTTTTACGTGTGCGTCATCTTGTCAcc                       >  1:2573665/1‑93 (MQ=255)
                                                                    gCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCg                     >  1:3770033/1‑93 (MQ=255)
                                                                     cTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGGGAACCTGTTTTACGAGTGCGTAATCTTTTCACCCGt                    >  2:2617358/1‑93 (MQ=255)
                                                                     cTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGt                    <  1:1180166/93‑1 (MQ=255)
                                                                      ttGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGtt                   <  2:5813617/93‑1 (MQ=255)
                                                                      ttGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGtt                   <  1:5245511/93‑1 (MQ=255)
                                                                      ttGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGACGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGtt                   >  2:546366/1‑93 (MQ=255)
                                                                       tGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGttt                  <  1:4116230/93‑1 (MQ=255)
                                                                        gAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGGACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTc                 >  2:742948/1‑93 (MQ=255)
                                                                        gAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTc                 >  1:458645/1‑93 (MQ=255)
                                                                          aCATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTccc               <  1:2229739/93‑1 (MQ=255)
                                                                          aCATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTccc               >  2:1193490/1‑93 (MQ=255)
                                                                           cATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCt              <  2:2383267/93‑1 (MQ=255)
                                                                            aTCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCtt             <  2:4415778/93‑1 (MQ=255)
                                                                            aTCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCtt             >  2:3114145/1‑93 (MQ=255)
                                                                              ccAGCGAAACAG‑CCGCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTg           <  2:3197100/93‑1 (MQ=255)
                                                                              ccAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTg           <  2:45083/93‑1 (MQ=255)
                                                                              ccAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTg           <  2:6432657/93‑1 (MQ=255)
                                                                               cAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgc          <  1:2838565/93‑1 (MQ=255)
                                                                                 gCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgcgc        <  2:5635776/93‑1 (MQ=255)
                                                                                 gCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgcgc        <  1:588947/93‑1 (MQ=255)
                                                                                  cGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCa       <  2:2346981/93‑1 (MQ=255)
                                                                                   gAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTTCCTTTGCGCAg      >  2:10772/1‑93 (MQ=255)
                                                                                    aaaCAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGc     >  2:3329167/1‑93 (MQ=255)
                                                                                     aaCAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg    >  2:1771317/1‑93 (MQ=255)
                                                                                     aaCAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg    >  1:4911821/1‑93 (MQ=255)
                                                                                     aaCAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg    >  2:3986306/1‑93 (MQ=255)
                                                                                       cAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGt  <  1:112554/93‑1 (MQ=255)
                                                                                           |                                                                                         
TTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑GCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGT  >  NC_000913/867547‑867726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑GCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGT  >  NC_000913/867544‑867730
                                                                                              |                                                                                             
CTGTTAGCTGCTGTTCCGCAACTTGGTGCGGTGAACGGGTTAAATTATCCCCGAAGTTTCCCGTTGATATAGCTTGAACATCCAGCGAAACAG‑C                                                                                               >  SRR4302534.5189739/1‑94 (MQ=60)
CTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑C                                                                                               <  SRR4302534.6541128/94‑1 (MQ=60)
 TGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CC                                                                                              >  SRR4302534.6303439/1‑94 (MQ=60)
  GTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCC                                                                                             <  SRR4302534.4439543/94‑1 (MQ=60)
  GTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCC                                                                                             <  SRR4302534.4620863/94‑1 (MQ=60)
    TAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCC                                                                                           >  SRR4302534.3591102/1‑94 (MQ=60)
    TAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCC                                                                                           >  SRR4302534.3569641/1‑94 (MQ=60)
    TAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCC                                                                                           >  SRR4302534.1489755/1‑94 (MQ=60)
       CTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCC                                                                                        >  SRR4302534.408173/1‑94 (MQ=60)
        TGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCC                                                                                       <  SRR4302534.792885/94‑1 (MQ=60)
        TGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCC                                                                                       <  SRR4302534.5595808/94‑1 (MQ=60)
        TGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACA‑‑GCCCCCCCCA                                                                                      >  SRR4302534.5642095/1‑94 (MQ=60)
         GCTTTTCCGCACCTTGTTGCGATGAAAGGTTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTCAACATCCAGCGAAACAG‑CCCCCCCCCA                                                                                      <  SRR4302534.6526988/94‑1 (MQ=60)
             TTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGA                                                                                  >  SRR4302534.816125/1‑94 (MQ=60)
              TCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAG                                                                                 <  SRR4302534.1561986/94‑1 (MQ=60)
              TCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAG                                                                                 <  SRR4302534.1767509/94‑1 (MQ=60)
               CCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGC                                                                                >  SRR4302534.1062108/1‑94 (MQ=60)
                 GCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGCAACAG‑ACACCCCCCATCGAGCCG                                                                              >  SRR4302534.2514759/1‑94 (MQ=60)
                 GCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCCTCGAGCAA                                                                              >  SRR4302534.3210352/1‑94 (MQ=60)
                 GCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAG                                                                              >  SRR4302534.4923676/1‑94 (MQ=60)
                  CAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGATCATCCAGCGAAACAG‑CCCCCCCACCTCGAGAAGA                                                                             >  SRR4302534.3914086/1‑94 (MQ=60)
                     CTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAA                                                                          >  SRR4302534.4744031/1‑94 (MQ=60)
                        TGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGG                                                                       >  SRR4302534.6414478/1‑94 (MQ=60)
                        GGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCCTCGAGCAGAAAACGG                                                                       >  SRR4302534.84441/1‑94 (MQ=60)
                         GTGCGAGAAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGT                                                                      <  SRR4302534.3510727/94‑1 (MQ=60)
                          GGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTG                                                                     <  SRR4302534.1188859/94‑1 (MQ=60)
                            CGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGT                                                                   >  SRR4302534.2731555/1‑94 (MQ=60)
                            CGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGG                                                                   >  SRR4302534.6579181/1‑94 (MQ=60)
                            CGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGG                                                                   >  SRR4302534.13598/1‑94 (MQ=60)
                             GATGAAAGGGTTAGATTATCCCCGGCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTG                                                                  <  SRR4302534.2406125/94‑1 (MQ=60)
                               TGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGA                                                                >  SRR4302534.19561/1‑94 (MQ=60)
                                GAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGCCCAGAAAACGGTGGaggcg                                                               >  SRR4302534.3975766/1‑89 (MQ=60)
                                GAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGAT                                                               >  SRR4302534.6309723/1‑94 (MQ=60)
                                 AAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATG                                                              >  SRR4302534.5679215/1‑94 (MQ=60)
                                 AAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATG                                                              <  SRR4302534.766742/94‑1 (MQ=60)
                                 AAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTAGTGGATG                                                              <  SRR4302534.1899534/94‑1 (MQ=60)
                                 AAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGCGGTGGATG                                                              >  SRR4302534.5235853/1‑94 (MQ=60)
                                  AAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGG                                                             <  SRR4302534.1202045/94‑1 (MQ=60)
                                  AAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGG                                                             >  SRR4302534.5193067/1‑94 (MQ=60)
                                  AAGGGTAAGATTATCCCCGACCTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGGAGAAAACGGTGGTGGATGG                                                             <  SRR4302534.6133329/94‑1 (MQ=60)
                                     GGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGCGCAGAAAACGGTGGTGGATGGCGA                                                          >  SRR4302534.1111580/1‑94 (MQ=60)
                                         AGATTATCCCCGCCGTTTCCCGTTGATATCGCGTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCT                                                      <  SRR4302534.4555872/94‑1 (MQ=60)
                                         AGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCT                                                      >  SRR4302534.1962371/1‑94 (MQ=60)
                                          GATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTG                                                     <  SRR4302534.739253/94‑1 (MQ=60)
                                            TTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAGCAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTT                                                   >  SRR4302534.51362/1‑94 (MQ=60)
                                            TTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTT                                                   <  SRR4302534.3510411/94‑1 (MQ=60)
                                            TTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTT                                                   >  SRR4302534.3698564/1‑94 (MQ=60)
                                            TTACCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCAAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTT                                                   <  SRR4302534.1261193/94‑1 (MQ=60)
                                              ATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTT                                                 <  SRR4302534.4587497/94‑1 (MQ=60)
                                                CCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAC                                               >  SRR4302534.4721276/1‑94 (MQ=60)
                                                 CCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAACTGTTTTACG                                              >  SRR4302534.5719195/1‑94 (MQ=60)
                                                   CGGCGTTTCCCGTTGATATCGCTTGAACAGCCAGCGAAACAG‑CCCACCCCCATCGAGCAGAAAACCGTGGTGGATGGCTATCCTGTTTTACGAG                                            <  SRR4302534.5998626/94‑1 (MQ=60)
                                                   CGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGCCCCCCCCCCATCGAGCAGAAAACGGGGGGGGGGGGGGAACCTGTTTTACGA                                             >  SRR4302534.3692111/1‑94 (MQ=60)
                                                     ACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTG                                          <  SRR4302534.3338704/94‑1 (MQ=60)
                                                      CGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGC                                         <  SRR4302534.3925409/94‑1 (MQ=60)
                                                      CGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGC                                         >  SRR4302534.919679/1‑94 (MQ=60)
                                                      CGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGC                                         >  SRR4302534.3393693/1‑94 (MQ=60)
                                                       GTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCG                                        >  SRR4302534.2369225/1‑94 (MQ=60)
                                                         TTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTA                                      >  SRR4302534.4764531/1‑94 (MQ=60)
                                                         TTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTA                                      >  SRR4302534.1040385/1‑94 (MQ=60)
                                                         TTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTA                                      >  SRR4302534.2425954/1‑94 (MQ=60)
                                                         TTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAAGAGTGCGTA                                      <  SRR4302534.6471091/94‑1 (MQ=60)
                                                          TCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAA                                     >  SRR4302534.65463/1‑94 (MQ=60)
                                                          TCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGAGAACCTGTTTTACGAGTGCGTAA                                     >  SRR4302534.5466095/1‑94 (MQ=60)
                                                           CCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAAT                                    <  SRR4302534.1174566/94‑1 (MQ=60)
                                                           CCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAAT                                    <  SRR4302534.2112294/94‑1 (MQ=60)
                                                            cctgttATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGGAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATC                                   <  SRR4302534.419473/88‑1 (MQ=60)
                                                            CCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATC                                   >  SRR4302534.3570155/1‑94 (MQ=60)
                                                            CCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATC                                   <  SRR4302534.1847518/94‑1 (MQ=60)
                                                            CCGTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATC                                   <  SRR4302534.2138635/94‑1 (MQ=60)
                                                             catcGATATCTCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCT                                  <  SRR4302534.5497450/90‑1 (MQ=60)
                                                             CGTTGATATCGCTTGAACATCCAGCGAAACAG‑CACCCCCCCATCGAGCAGAAAACGGGGGTGGATGGCGGACCTGTTTTACGGGTGCGTAATCT                                  >  SRR4302534.1077427/1‑94 (MQ=60)
                                                              GTTGATATCGCTTGACCATCCAGCGAAACAC‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTT                                 <  SRR4302534.3808746/94‑1 (MQ=60)
                                                              GTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACCAGTGAGTAATCTT                                 >  SRR4302534.6050211/1‑94 (MQ=60)
                                                              GTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGACGGCGAACCTGGTTTACGAGTGCGTAATCTT                                 >  SRR4302534.1043780/1‑94 (MQ=60)
                                                              GTTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGAGGATGGGGGACCTGTTTTACGAGTGTGTCATCTT                                 >  SRR4302534.4785313/1‑94 (MQ=60)
                                                               TTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTT                                >  SRR4302534.324915/1‑94 (MQ=60)
                                                               TTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTG                                <  SRR4302534.2083537/94‑1 (MQ=60)
                                                               TTGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTG                                <  SRR4302534.4374102/94‑1 (MQ=60)
                                                                TGATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGT                               >  SRR4302534.650609/1‑94 (MQ=60)
                                                                TGATATCGCTTGAACATCCAACGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGT                               >  SRR4302534.202183/1‑94 (MQ=60)
                                                                 TATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTC                              <  SRR4302534.316872/94‑1 (MQ=60)
                                                                  ATCTCGCTGGAACATGCAGGGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCATAATCTTGTCA                             <  SRR4302534.5164470/94‑1 (MQ=60)
                                                                  ATATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTTA                             >  SRR4302534.2467442/1‑94 (MQ=60)
                                                                   TATCGCTTGAACATCCAGCGGAACAG‑ACCCCCCCCATTGAGCAGAAAAaagttggggatggcgaacccctttttcggctgcggaaacttttaca                            >  SRR4302534.4898967/1‑48 (MQ=60)
                                                                   TATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAC                            <  SRR4302534.5249190/94‑1 (MQ=60)
                                                                    ATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACC                           <  SRR4302534.3440685/94‑1 (MQ=60)
                                                                    ATCGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGAGGATGGCGAACCTGTTTTACGTGTGCGTCATCTTGTCACC                           >  SRR4302534.2641848/1‑94 (MQ=60)
                                                                      CGCTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCG                         >  SRR4302534.3871272/1‑94 (MQ=60)
                                                                        CTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTT                       >  SRR4302534.2427066/1‑94 (MQ=60)
                                                                        CTTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTT                       <  SRR4302534.1211187/94‑1 (MQ=60)
                                                                         TTGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTT                      <  SRR4302534.5387198/94‑1 (MQ=60)
                                                                          TGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTC                     >  SRR4302534.470237/1‑94 (MQ=60)
                                                                          TGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTC                     <  SRR4302534.4226977/94‑1 (MQ=60)
                                                                          TGAACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTC                     <  SRR4302534.3641874/94‑1 (MQ=60)
                                                                            AACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAACCGTTTCCC                   >  SRR4302534.3848440/1‑94 (MQ=60)
                                                                             ACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCT                  <  SRR4302534.572696/94‑1 (MQ=60)
                                                                             ACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCT                  <  SRR4302534.2288801/94‑1 (MQ=60)
                                                                             ACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCT                  <  SRR4302534.2304740/94‑1 (MQ=60)
                                                                             ACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCT                  <  SRR4302534.2976338/94‑1 (MQ=60)
                                                                             ACATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACCAGTGCGTAATCTTGTCACCCGTTTCCCT                  >  SRR4302534.1990498/1‑94 (MQ=60)
                                                                              CATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACCAGAGCGTAATCTTGTCACCCGTTTCCCTT                 >  SRR4302534.5208362/1‑94 (MQ=60)
                                                                               ATCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGGGGTGGATGGCGAACCTGTTTTACGAGTGCGGAATCTTGTCACCCGTGTCCCTTT                >  SRR4302534.2032472/1‑94 (MQ=60)
                                                                                TCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTG               >  SRR4302534.2174682/1‑94 (MQ=60)
                                                                                TCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTG               <  SRR4302534.4542397/94‑1 (MQ=60)
                                                                                TCCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTG               <  SRR4302534.729731/94‑1 (MQ=60)
                                                                                 CCAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGC              >  SRR4302534.3378229/1‑94 (MQ=60)
                                                                                  CAGCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCG             <  SRR4302534.2913149/94‑1 (MQ=60)
                                                                                    GCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCA           >  SRR4302534.4338262/1‑94 (MQ=60)
                                                                                    GCGAAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCA           <  SRR4302534.603324/94‑1 (MQ=60)
                                                                                     CGAAACA‑‑GCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGG         >  SRR4302534.5283958/1‑94 (MQ=60)
                                                                                       AAACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCG        >  SRR4302534.5044942/1‑94 (MQ=60)
                                                                                        AACAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGG       <  SRR4302534.1135595/94‑1 (MQ=60)
                                                                                          CAG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTT     <  SRR4302534.115554/94‑1 (MQ=60)
                                                                                           AG‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCCGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTT    >  SRR4302534.659893/1‑94 (MQ=60)
                                                                                            G‑CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTG   >  SRR4302534.6628142/1‑94 (MQ=60)
                                                                                              CCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGT  >  SRR4302534.449757/1‑94 (MQ=60)
                                                                                              |                                                                                             
CTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAG‑GCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGT  >  NC_000913/867544‑867730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑