Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 928,190 G→A R77C (CGC→TGC)  cydC ← ATP‑binding component of cytochrome‑related transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913928,1900GA99.5% 775.1 / NA 218R77C (CGC→TGC) cydCATP‑binding component of cytochrome‑related transport
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (116/101);  minor base C (1/0);  total (117/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 222 total aligned reads displayed.

AGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCG  >  NC_000913/928100‑928279
                                                                                          |                                                                                         
 gagGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCAccc                                                                                        <  1:4016626/93‑1 (MQ=255)
 gagGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCAccc                                                                                        >  2:2894385/1‑93 (MQ=255)
 gagGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCAccc                                                                                        >  2:5641053/1‑93 (MQ=255)
  agGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCg                                                                                       >  1:4586999/1‑93 (MQ=255)
  agGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCg                                                                                       <  1:2799837/93‑1 (MQ=255)
  agGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCg                                                                                       <  2:447589/93‑1 (MQ=255)
   ggggCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCgg                                                                                      >  2:1449992/1‑93 (MQ=255)
    gggCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGc                                                                                     <  2:1760876/93‑1 (MQ=255)
        agcaATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTa                                                                                 >  2:1729485/1‑93 (MQ=255)
         gcaATTTGCTGAAGGGGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACACGGCCGTAc                                                                                >  1:3890511/1‑93 (MQ=255)
          caATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACg                                                                               <  2:1391731/93‑1 (MQ=255)
           aaTTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACgg                                                                              >  1:2236901/1‑93 (MQ=255)
            aTTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACggg                                                                             >  1:3961921/1‑93 (MQ=255)
               tGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGa                                                                          >  2:159689/1‑93 (MQ=255)
                gCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGAt                                                                         <  2:4724684/93‑1 (MQ=255)
                  tGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATtg                                                                       <  1:2659660/93‑1 (MQ=255)
                   gAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATtgc                                                                      >  2:3780765/1‑93 (MQ=255)
                      ggTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATtgctgc                                                                   <  1:2513345/93‑1 (MQ=255)
                       gtgtAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTgcg                                                                  >  2:3742470/1‑93 (MQ=255)
                        tgtAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTgcgc                                                                 <  2:2733393/93‑1 (MQ=255)
                           aaaTGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCcac                                                              >  2:4407104/1‑93 (MQ=255)
                           aaaTGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCcac                                                              >  1:5446913/1‑93 (MQ=255)
                            aaTGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCcacg                                                             >  2:319068/1‑93 (MQ=255)
                             aTGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCcacgc                                                            >  2:2934114/1‑93 (MQ=255)
                               gcgcAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCcacgcac                                                          >  2:3602250/1‑93 (MQ=255)
                               gcgcAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCcacgcac                                                          <  1:4512714/93‑1 (MQ=255)
                                 gcAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCacgcacgc                                                        >  1:1412307/1‑93 (MQ=255)
                                 gcAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCacgcacgc                                                        >  2:6449347/1‑93 (MQ=255)
                                 gcAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCacgcacgc                                                        >  1:173249/1‑93 (MQ=255)
                                  cAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTTCTGCGCCACGCACGcc                                                       <  2:538858/93‑1 (MQ=255)
                                   aGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGccc                                                      >  2:6012256/1‑93 (MQ=255)
                                   aGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGccc                                                      >  2:6294240/1‑93 (MQ=255)
                                   aGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGccc                                                      <  1:2157163/93‑1 (MQ=255)
                                     aTGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGc                                                    >  2:5618160/1‑93 (MQ=255)
                                        ctgcAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGc                                                 <  2:5276601/93‑1 (MQ=255)
                                         tgcAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCg                                                <  2:5736808/93‑1 (MQ=255)
                                         tgcAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCg                                                <  1:635490/93‑1 (MQ=255)
                                          gcAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCgg                                               >  1:2710836/1‑93 (MQ=255)
                                            aaCACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGt                                             <  2:4277046/93‑1 (MQ=255)
                                            aaCACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCACGCAGCGGGt                                             >  1:1029370/1‑93 (MQ=255)
                                            aaCACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGAACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGt                                             >  2:2554343/1‑93 (MQ=255)
                                             acacGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTa                                            >  1:1865143/1‑93 (MQ=255)
                                             acacGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTa                                            >  2:5578187/1‑93 (MQ=255)
                                             acacGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTa                                            >  1:5667727/1‑93 (MQ=255)
                                             acacGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTa                                            >  2:1198795/1‑93 (MQ=255)
                                             acacGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTa                                            <  1:5549220/93‑1 (MQ=255)
                                             acacGCGGAAAGTCGCGTCGTGACTTACCAGACGGTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGGCACGCCAGGCCGCCGCgggga                                            >  1:97403/1‑91 (MQ=255)
                                              cacGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAg                                           >  1:3191201/1‑93 (MQ=255)
                                               acGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGc                                          >  2:5118870/1‑93 (MQ=255)
                                               acGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGc                                          <  1:5731788/93‑1 (MQ=255)
                                               acGCGGAAAGCCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGc                                          <  1:6078141/93‑1 (MQ=255)
                                                cgcgGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCa                                         >  2:4148591/1‑93 (MQ=255)
                                                 gcgGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCat                                        <  1:2487291/93‑1 (MQ=255)
                                                  tggAAAGACGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCata                                       <  2:3536724/92‑1 (MQ=255)
                                                  cgGAAAGTCGCGTCCTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGAAGCata                                       >  1:5971658/1‑93 (MQ=255)
                                                   ggAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCatat                                      >  2:2484814/1‑93 (MQ=255)
                                                    gAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCAtata                                     >  1:5931910/1‑93 (MQ=255)
                                                    gAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCAtata                                     >  1:3435852/1‑93 (MQ=255)
                                                     aaaGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAg                                    >  2:3432105/1‑93 (MQ=255)
                                                      aaGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGt                                   <  1:625946/93‑1 (MQ=255)
                                                      aaGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGt                                   <  1:3845422/93‑1 (MQ=255)
                                                      aaGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGt                                   <  2:6196274/93‑1 (MQ=255)
                                                      aaGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGAAGCAtatact                                   >  2:231855/1‑91 (MQ=255)
                                                       aGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGtt                                  <  2:2382875/93‑1 (MQ=255)
                                                       aGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGtt                                  >  2:3918692/1‑93 (MQ=255)
                                                        gTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGGCCGCAGCGGGTAGCATATAGTTg                                 >  2:3804164/1‑93 (MQ=255)
                                                          cgcgTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGaa                               <  1:1563277/93‑1 (MQ=255)
                                                           gcgTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAg                              <  2:6048017/93‑1 (MQ=255)
                                                           gcgTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAg                              >  2:4842352/1‑93 (MQ=255)
                                                            cgtcgtGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGc                             <  1:4974655/93‑1 (MQ=255)
                                                             gtcgtGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCt                            <  1:5103931/93‑1 (MQ=255)
                                                             gtcgtGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCt                            <  1:5658036/93‑1 (MQ=255)
                                                             gtcgtGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCt                            >  1:1313670/1‑93 (MQ=255)
                                                             gtcgtGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCt                            <  1:3608597/93‑1 (MQ=255)
                                                              tcgtGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTg                           >  1:1050328/1‑93 (MQ=255)
                                                                gtGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTa                         >  2:4553540/1‑93 (MQ=255)
                                                                 tGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTAc                        <  1:4407501/93‑1 (MQ=255)
                                                                 tGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTAc                        <  1:2323173/93‑1 (MQ=255)
                                                                  gACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACa                       >  1:5289170/1‑93 (MQ=255)
                                                                  gACTTACCAGACGGTCAAAATAGCACCCGGCAGTGCGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGGAGCATATAGTTGAAGCTGTACa                       >  1:297611/1‑93 (MQ=255)
                                                                    cTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGt                     <  1:114376/93‑1 (MQ=255)
                                                                     ttACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTc                    <  2:2264432/93‑1 (MQ=255)
                                                                      tACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTcc                   <  1:354976/93‑1 (MQ=255)
                                                                      tACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTcc                   >  1:4831489/1‑93 (MQ=255)
                                                                      tACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTcc                   <  2:5841074/93‑1 (MQ=255)
                                                                       aCCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCg                  >  1:83247/1‑93 (MQ=255)
                                                                         cAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCgga                >  2:2834499/1‑92 (MQ=255)
                                                                         cAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGc                <  2:2174917/93‑1 (MQ=255)
                                                                         cAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGc                >  1:796819/1‑93 (MQ=255)
                                                                          aGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCa               >  1:3037805/1‑93 (MQ=255)
                                                                           gACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCaa              >  2:5868396/1‑93 (MQ=255)
                                                                           gACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCaa              <  1:5782740/93‑1 (MQ=255)
                                                                            aCGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAAc             <  2:4895568/93‑1 (MQ=255)
                                                                            aCGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAAc             <  2:861617/93‑1 (MQ=255)
                                                                                tCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAAccccc         >  1:4606891/1‑93 (MQ=255)
                                                                                 cAAAATAGCCCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCg        >  1:4221426/1‑93 (MQ=255)
                                                                                   aaaTAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCa      >  2:1882388/1‑93 (MQ=255)
                                                                                     aTAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAAc    <  1:663882/93‑1 (MQ=255)
                                                                                     aTAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAAc    <  1:5901818/93‑1 (MQ=255)
                                                                                     aTAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAAc    <  1:1905304/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCG  >  NC_000913/928100‑928279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCT  >  NC_000913/928099‑928281
                                                                                           |                                                                                           
GAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACC                                                                                           >  SRR4302534.5920101/1‑94 (MQ=60)
GAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACC                                                                                           <  SRR4302534.410008/94‑1 (MQ=60)
GAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACC                                                                                           <  SRR4302534.3485334/94‑1 (MQ=60)
 AGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCC                                                                                          >  SRR4302534.1583926/1‑94 (MQ=60)
  GAGGGTCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCG                                                                                         <  SRR4302534.5577481/94‑1 (MQ=60)
  GAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCG                                                                                         >  SRR4302534.5974779/1‑94 (MQ=60)
  GAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCG                                                                                         >  SRR4302534.4711473/1‑94 (MQ=60)
  GAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCG                                                                                         <  SRR4302534.4124294/94‑1 (MQ=60)
  GAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCG                                                                                         <  SRR4302534.118364/94‑1 (MQ=60)
   AGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGG                                                                                        <  SRR4302534.2873475/94‑1 (MQ=60)
     GGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCA                                                                                      <  SRR4302534.2744956/94‑1 (MQ=60)
     GGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCA                                                                                      <  SRR4302534.3529054/94‑1 (MQ=60)
      GGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAG                                                                                     <  SRR4302534.1020550/94‑1 (MQ=60)
      GGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAG                                                                                     >  SRR4302534.4057406/1‑94 (MQ=60)
         AGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTAC                                                                                  >  SRR4302534.1916808/1‑94 (MQ=60)
         AGCAATTTGCTGAAGGGGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACACGGCCGTAC                                                                                  >  SRR4302534.3994948/1‑94 (MQ=60)
          GCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACG                                                                                 >  SRR4302534.6227301/1‑94 (MQ=60)
           CAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGG                                                                                <  SRR4302534.3091254/94‑1 (MQ=60)
           CAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGG                                                                                >  SRR4302534.2296175/1‑94 (MQ=60)
            AATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGG                                                                               >  SRR4302534.4067995/1‑94 (MQ=60)
             ATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGT                                                                              <  SRR4302534.4850086/94‑1 (MQ=60)
             ATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGG                                                                              >  SRR4302534.1062559/1‑94 (MQ=60)
              TTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTG                                                                             <  SRR4302534.2562476/94‑1 (MQ=60)
              TTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTG                                                                             <  SRR4302534.4360320/94‑1 (MQ=60)
                  CTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTG                                                                         <  SRR4302534.5404042/94‑1 (MQ=60)
                   TGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGC                                                                        <  SRR4302534.2729975/94‑1 (MQ=60)
                    GAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACTCGGCAGTACGGGTGATTGCT                                                                       <  SRR4302534.4076752/94‑1 (MQ=60)
                    GAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCT                                                                       <  SRR4302534.4721526/94‑1 (MQ=60)
                    GAAGGTGTAAATGCGCAGATGCTGAAACACGCGGCAAGTCGCGTCGTGACTTACCAGACGGTCAAAATAGCACCCGTCAGTCCGGATGATTGCT                                                                       >  SRR4302534.1028275/1‑94 (MQ=60)
                     AAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTG                                                                      <  SRR4302534.6084214/94‑1 (MQ=60)
                       GGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCG                                                                    <  SRR4302534.2580075/94‑1 (MQ=60)
                           TAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCAC                                                                >  SRR4302534.5593670/1‑94 (MQ=60)
                           TAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTCCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCAC                                                                >  SRR4302534.3505996/1‑94 (MQ=60)
                            AAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACG                                                               >  SRR4302534.2936261/1‑94 (MQ=60)
                            AAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACG                                                               >  SRR4302534.1869808/1‑94 (MQ=60)
                              ATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCA                                                             >  SRR4302534.4732390/1‑94 (MQ=60)
                                GCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACG                                                           <  SRR4302534.4634939/94‑1 (MQ=60)
                                GCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACG                                                           >  SRR4302534.6370808/1‑94 (MQ=60)
                                GCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACG                                                           >  SRR4302534.211393/1‑94 (MQ=60)
                                 CGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGC                                                          >  SRR4302534.177814/1‑94 (MQ=60)
                                 CGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGC                                                          >  SRR4302534.1449459/1‑94 (MQ=60)
                                 CGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGC                                                          >  SRR4302534.282385/1‑94 (MQ=60)
                                 CGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACACGGCAGTACtgcggcttgcttcgacacacacac                                                          >  SRR4302534.6002197/1‑70 (MQ=60)
                                    AGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCG                                                       <  SRR4302534.2214115/94‑1 (MQ=60)
                                     GATGCTGCAACACGCGGAAAGTCGCGTCGTGGCTTACCAGACGTTCAAAATAGCACTCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGC                                                      >  SRR4302534.826725/1‑94 (MQ=60)
                                      ATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCA                                                     <  SRR4302534.726859/94‑1 (MQ=60)
                                       TGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAG                                                    <  SRR4302534.1676743/94‑1 (MQ=60)
                                         CTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCG                                                  <  SRR4302534.5811470/94‑1 (MQ=60)
                                          TGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGG                                                 <  SRR4302534.3158563/94‑1 (MQ=60)
                                          TGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGG                                                 >  SRR4302534.2782413/1‑94 (MQ=60)
                                          TGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGG                                                 <  SRR4302534.650913/94‑1 (MQ=60)
                                           TCAACACGCTGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGG                                                <  SRR4302534.3738251/94‑1 (MQ=60)
                                           GCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGG                                                <  SRR4302534.5932776/94‑1 (MQ=60)
                                            CAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGT                                               <  SRR4302534.4396363/94‑1 (MQ=60)
                                            CAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGT                                               <  SRR4302534.6551431/94‑1 (MQ=60)
                                            CAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCACGCAGCGGGT                                               >  SRR4302534.1055888/1‑94 (MQ=60)
                                             AACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTA                                              >  SRR4302534.1913696/1‑94 (MQ=60)
                                             AACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTA                                              >  SRR4302534.5821165/1‑94 (MQ=60)
                                             AACACGCGGAAAGTCGCGTCGTGACTTACCAGACGGTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGGCACGCcaggccgccgcgggga                                              >  SRR4302534.100007/1‑78 (MQ=60)
                                              ACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAG                                             <  SRR4302534.5699003/94‑1 (MQ=60)
                                              ACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAG                                             <  SRR4302534.5822008/94‑1 (MQ=60)
                                              ACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAG                                             >  SRR4302534.3275700/1‑94 (MQ=60)
                                               CACGCGGAAAGTCCCGTCGTGGCTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGGCACGCACGCGCGCAGCGGGGAGC                                            >  SRR4302534.5664341/1‑94 (MQ=60)
                                                ACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCA                                           <  SRR4302534.5887106/94‑1 (MQ=60)
                                                ACGCGGAAAGCCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCA                                           <  SRR4302534.6243429/94‑1 (MQ=60)
                                                 CGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCAT                                          >  SRR4302534.3767439/1‑94 (MQ=60)
                                                  GCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATA                                         <  SRR4302534.2553370/94‑1 (MQ=60)
                                                  GCGGAAAGTCGCGTCCTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGAAGCATA                                         >  SRR4302534.6133848/1‑94 (MQ=60)
                                                   CGGAAAGTCGCGTCGTGACTTCCCAGACGTTCAAAATAGCACCCGGCAGTACGGGGGATTGCTGAGCCACGCACGCCCGCCGCGGGTAGCATAT                                        >  SRR4302534.3688105/1‑94 (MQ=60)
                                                   CGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATAT                                        >  SRR4302534.3123574/1‑94 (MQ=60)
                                                    GGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATA                                       >  SRR4302534.3528186/1‑94 (MQ=60)
                                                    GGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATA                                       <  SRR4302534.855503/94‑1 (MQ=60)
                                                    GGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATA                                       >  SRR4302534.6092996/1‑94 (MQ=60)
                                                       AAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTT                                    <  SRR4302534.3948697/94‑1 (MQ=60)
                                                       AAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTT                                    <  SRR4302534.641130/94‑1 (MQ=60)
                                                        AGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTG                                   <  SRR4302534.6367761/94‑1 (MQ=60)
                                                        AGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTG                                   <  SRR4302534.5092076/94‑1 (MQ=60)
                                                           agcgtcgtggctatCCAGATGTTCAAAATAGCACCCGGCAGTACGGGTGATTGTTTCGCCACGCACTCCCGCAGCGGGTAGCATATAGTTGAAG                                <  SRR4302534.3153779/80‑1 (MQ=60)
                                                           CGCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAG                                <  SRR4302534.1604193/94‑1 (MQ=60)
                                                            GCGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGC                               >  SRR4302534.2280165/1‑94 (MQ=60)
                                                             CGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCT                              >  SRR4302534.1348306/1‑94 (MQ=60)
                                                             CGTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCT                              <  SRR4302534.5109323/94‑1 (MQ=60)
                                                              GTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTG                             <  SRR4302534.5241682/94‑1 (MQ=60)
                                                              GTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTG                             >  SRR4302534.1077526/1‑94 (MQ=60)
                                                              GTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTG                             <  SRR4302534.3705640/94‑1 (MQ=60)
                                                              GTCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTG                             <  SRR4302534.5811214/94‑1 (MQ=60)
                                                               TCGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGT                            <  SRR4302534.2713844/94‑1 (MQ=60)
                                                                CGTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTA                           >  SRR4302534.2235049/1‑94 (MQ=60)
                                                                 GTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTAC                          >  SRR4302534.2602507/1‑94 (MQ=60)
                                                                 GTGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTAC                          >  SRR4302534.5780672/1‑94 (MQ=60)
                                                                  TGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACA                         >  SRR4302534.5431942/1‑94 (MQ=60)
                                                                  TGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACA                         <  SRR4302534.2384828/94‑1 (MQ=60)
                                                                  TGACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACA                         <  SRR4302534.4526451/94‑1 (MQ=60)
                                                                  TGACTTACCAGACGGTCAAAATAGCACCCGGCAGTGCGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGGAGCATATAGTTGAAGCTGTACA                         >  SRR4302534.305304/1‑94 (MQ=60)
                                                                   GACTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAG                        >  SRR4302534.2353318/1‑94 (MQ=60)
                                                                     CTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTC                      <  SRR4302534.5619764/94‑1 (MQ=60)
                                                                     CTTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTC                      <  SRR4302534.117423/94‑1 (MQ=60)
                                                                      TTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCC                     >  SRR4302534.4962568/1‑94 (MQ=60)
                                                                      TTACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCC                     <  SRR4302534.4669691/94‑1 (MQ=60)
                                                                       TACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCG                    >  SRR4302534.85476/1‑94 (MQ=60)
                                                                       TACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCG                    <  SRR4302534.364057/94‑1 (MQ=60)
                                                                       TACCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCG                    >  SRR4302534.4627892/1‑94 (MQ=60)
                                                                         CCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGC                  >  SRR4302534.816820/1‑94 (MQ=60)
                                                                         CCAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGGTGAAGCTGTACAGTCCGGC                  >  SRR4302534.1099684/1‑94 (MQ=60)
                                                                          CAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCA                 >  SRR4302534.3117462/1‑94 (MQ=60)
                                                                          CAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCA                 >  SRR4302534.1582108/1‑94 (MQ=60)
                                                                          CAGACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCA                 <  SRR4302534.649750/94‑1 (MQ=60)
                                                                            GACGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAAC               <  SRR4302534.5939603/94‑1 (MQ=60)
                                                                              CGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCC             >  SRR4302534.3953617/1‑94 (MQ=60)
                                                                              CGTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCC             <  SRR4302534.847090/94‑1 (MQ=60)
                                                                               GTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCC            <  SRR4302534.6099896/94‑1 (MQ=60)
                                                                               GTTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCC            <  SRR4302534.2432217/94‑1 (MQ=60)
                                                                                TTCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCC           >  SRR4302534.4731908/1‑94 (MQ=60)
                                                                                 TCAAAATAGCCCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCG          >  SRR4302534.4335228/1‑94 (MQ=60)
                                                                                 TCAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCG          <  SRR4302534.1379505/94‑1 (MQ=60)
                                                                                  CAAAATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGC         <  SRR4302534.5112169/94‑1 (MQ=60)
                                                                                      ATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACC     <  SRR4302534.1955005/94‑1 (MQ=60)
                                                                                      ATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACC     <  SRR4302534.680043/94‑1 (MQ=60)
                                                                                      ATAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACC     <  SRR4302534.6062059/94‑1 (MQ=60)
                                                                                       TAGCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCG    >  SRR4302534.4126478/1‑94 (MQ=60)
                                                                                         GCACCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTACGGCAACCCCCGCAACCGCT  >  SRR4302534.5936481/1‑94 (MQ=60)
                                                                                           |                                                                                           
GAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAGTCCGGCAACCCCCGCAACCGCT  >  NC_000913/928099‑928281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑