Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 934,731 A→G Q762R (CAG→CGG)  ftsK → cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913934,7310AG99.4% 512.7 / ‑4.5 161Q762R (CAG→CGG) ftsKcell division protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (100/59);  total (102/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01

Only 100 of 222 total aligned reads displayed.

TTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATC  >  NC_000913/934641‑934820
                                                                                          |                                                                                         
ttACGCCAATTGTTGACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcggc                                                                                         <  1:3552375/93‑1 (MQ=255)
ttACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcggc                                                                                         <  2:1454934/93‑1 (MQ=255)
 tACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcggcc                                                                                        <  1:2507289/93‑1 (MQ=255)
  aCGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcggccg                                                                                       >  1:4217705/1‑93 (MQ=255)
   cGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcggccgc                                                                                      <  2:6208390/93‑1 (MQ=255)
   cGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcggccgc                                                                                      <  2:7546859/93‑1 (MQ=255)
       aaTTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTa                                                                                  >  1:3971575/1‑93 (MQ=255)
       aaTTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTa                                                                                  <  2:1934465/93‑1 (MQ=255)
       aaTTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTa                                                                                  <  1:2803764/93‑1 (MQ=255)
        aTTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTAt                                                                                 <  1:5160309/93‑1 (MQ=255)
        aTTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTAt                                                                                 <  1:8169755/93‑1 (MQ=255)
         ttgttgACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATc                                                                                <  2:4417673/93‑1 (MQ=255)
         ttgttgACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATc                                                                                <  2:3152035/93‑1 (MQ=255)
         ttgttgAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATc                                                                                >  1:8688954/1‑93 (MQ=255)
         ttgttgAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATc                                                                                >  2:2725999/1‑93 (MQ=255)
           tgtgACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATcag                                                                              <  2:4058379/91‑1 (MQ=255)
           gttgAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATcag                                                                              <  2:4214792/93‑1 (MQ=255)
           gttgAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATcag                                                                              <  1:1495738/93‑1 (MQ=255)
           gttgAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATcag                                                                              >  1:8322482/1‑93 (MQ=255)
            ttgAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATcagc                                                                             <  1:3505021/93‑1 (MQ=255)
              gAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATcagcac                                                                           >  1:7335618/1‑92 (MQ=255)
                aCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGcc                                                                         >  1:3255354/1‑93 (MQ=255)
                aCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGcc                                                                         >  1:5609338/1‑93 (MQ=255)
                aCCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGGATCaccagct                                                                         >  2:1149150/1‑92 (MQ=18)
                   tGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCcaca                                                                      >  1:7759945/1‑93 (MQ=255)
                    gTACAGCAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCacaa                                                                     <  1:2358326/93‑1 (MQ=255)
                    gTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCacaa                                                                     <  1:1124209/93‑1 (MQ=255)
                      aaggcaccCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCacaaca                                                                   <  1:7931741/90‑1 (MQ=17)
                       cagcagCCGCAACAACCGGTTGCACCGCAGCAGCACTATCAGCAGCCGCAACAACCAGTTCCACCGCAGCCGCAGTATCAGCAGCCACAACAg                                                                  >  2:1496256/1‑93 (MQ=255)
                        agcggcCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGc                                                                 <  1:4264993/93‑1 (MQ=255)
                        agcagcCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGc                                                                 <  1:8113116/93‑1 (MQ=255)
                        agcaccCGAAACACCCGGTTCCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGc                                                                 <  2:7715124/93‑1 (MQ=17)
                         gcagcCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGGATCAGCAGCTACAACAGcc                                                                >  2:6331046/1‑93 (MQ=255)
                             ccGCAACAACGGGTGGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGtt                                                            <  2:5451084/93‑1 (MQ=255)
                               gCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTgc                                                          >  2:2277112/1‑93 (MQ=255)
                               gCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTgc                                                          >  2:4085219/1‑93 (MQ=255)
                               gCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTgc                                                          >  1:509683/1‑93 (MQ=255)
                                 aacaacCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTgcgc                                                        >  2:6176254/1‑93 (MQ=255)
                                 aacaacCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTgcgc                                                        >  1:1203661/1‑93 (MQ=255)
                                  acaacCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGcgcc                                                       >  2:6505176/1‑93 (MQ=255)
                                  acaacCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGcgcc                                                       >  2:6809119/1‑93 (MQ=255)
                                   aaacCGGTTGCACCGCAGCAGCAATACCACCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGcgccg                                                      <  1:1330825/92‑1 (MQ=17)
                                    aacCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGcgccgc                                                     >  2:8680181/1‑93 (MQ=255)
                                    aacCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGcgccgc                                                     <  2:6465704/93‑1 (MQ=255)
                                    aacCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGagcagc                                                     >  1:4963848/1‑93 (MQ=255)
                                          ttGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCcac                                               >  1:6462181/1‑93 (MQ=255)
                                            gCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACaa                                             >  2:2120776/1‑93 (MQ=255)
                                            cccccGCAGCACCAATATCAGCAGCCGCAACAACCAGTCCCGCCGCGCCCGCATTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACaa                                             <  2:8278967/90‑1 (MQ=18)
                                             ctccGCAGCAGCAATACCAGCAGCCGCACCAGCCAGTTCCGCGTCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAat                                            <  2:5244009/91‑1 (MQ=18)
                                             cACCGCAGCAGAAATACCACCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAat                                            <  2:5011057/93‑1 (MQ=21)
                                               ccGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAatat                                          >  1:3921738/1‑93 (MQ=255)
                                                cGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATc                                         >  2:1202691/1‑93 (MQ=255)
                                                cGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATc                                         >  2:2597915/1‑93 (MQ=255)
                                                cGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCCGTTGCGCCGCAGCCACAATATc                                         >  2:1554655/1‑93 (MQ=255)
                                                 gcagcagcaATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATca                                        >  1:1767760/1‑93 (MQ=255)
                                                  cagcagcaATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATcag                                       <  2:1872824/93‑1 (MQ=255)
                                                   agcagcaATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATcagc                                      <  2:475268/93‑1 (MQ=255)
                                                   agcagcaACATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATcagc                                      <  1:654423/93‑1 (MQ=255)
                                                      agcaATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCagcagc                                   >  1:7231882/1‑93 (MQ=255)
                                                       gcaATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGcc                                  <  2:727700/93‑1 (MQ=255)
                                                         aaTATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGc                                >  1:8068432/1‑93 (MQ=255)
                                                         aaTATCAGCAGCCGCAACAACCAGTTCCGCCGCGCCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGc                                <  1:3056372/93‑1 (MQ=255)
                                                          atatCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGca                               >  2:39090/1‑93 (MQ=255)
                                                           tatCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGcaa                              >  2:6041405/1‑93 (MQ=255)
                                                           tacaagcagcCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGcaa                              <  1:4765137/89‑1 (MQ=255)
                                                           aaccagcggCCGCAACACCCAGTCCCGCCGCGGCCGCAGTACCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGcaa                              <  2:3746660/90‑1 (MQ=21)
                                                            atCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGcaac                             >  1:8769349/1‑93 (MQ=255)
                                                             tCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCTCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGcaaca                            <  1:8519443/93‑1 (MQ=255)
                                                               gccggcCGAACCAACCAGTCCCGCCGGGCCTGAGGTCTCCGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCaacaac                          <  2:60768/89‑1 (MQ=12)
                                                               cgcagcCCCACCACCCAGTCCCGCCGCGGCCGCAGTATCACCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCaacaac                          <  2:3156161/92‑1 (MQ=21)
                                                               agcagcCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCaacaac                          <  1:5447568/93‑1 (MQ=255)
                                                               agcagcCGCAACAACCAGTCCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCaacaac                          <  2:6101963/93‑1 (MQ=255)
                                                                  agcCGCAACACCCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCgg                       <  1:8389790/93‑1 (MQ=255)
                                                                   gcCGCAACACCCAGTCCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGt                      <  2:1860031/93‑1 (MQ=255)
                                                                   gcCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCGGCAACAACCGGt                      >  1:2223309/1‑93 (MQ=255)
                                                                   gcCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGt                      >  2:7248319/1‑93 (MQ=255)
                                                                   gcCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGt                      >  2:8232053/1‑93 (MQ=255)
                                                                      gCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTgc                   >  1:7029638/1‑93 (MQ=255)
                                                                       caacaaCCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTggc                  >  1:6836065/1‑91 (MQ=255)
                                                                       caacaaCCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTgcg                  >  1:5381598/1‑93 (MQ=255)
                                                                       caacaaCCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTgcg                  >  2:1089310/1‑93 (MQ=255)
                                                                        aacaacCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTgcgc                 >  1:1812598/1‑93 (MQ=255)
                                                                         acaccCAGTCCCCCGGCGGCAGCAGTATCACCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGcc                <  2:5613141/93‑1 (MQ=18)
                                                                         acaacCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGcc                >  2:1959761/1‑93 (MQ=255)
                                                                         acaacCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGcc                >  1:4479675/1‑93 (MQ=255)
                                                                          caacCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCca               >  1:7549844/1‑93 (MQ=255)
                                                                           accgaGTCCCGCCGCGGCCAAAGAATCAGCAGCAACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCcac              <  2:7955511/89‑1 (MQ=17)
                                                                           accaaGTCCCGCCGCGGCCGCATTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCcac              <  1:8012998/89‑1 (MQ=18)
                                                                           aacCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCcac              <  1:7451080/93‑1 (MQ=255)
                                                                           aacCAGTTCCCCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCcac              <  1:6279606/93‑1 (MQ=255)
                                                                            acCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCcaca             >  1:7179166/1‑93 (MQ=255)
                                                                             ccAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGCTGCGCCGCAGCCACAATATCAGCAGCCGCAACAGCCGGTTGCGCCAcag            >  2:385524/1‑93 (MQ=255)
                                                                              cAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCAcagc           >  1:6209464/1‑93 (MQ=255)
                                                                               cgccccgccgCGGCCGCAATAGCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCAcagca          <  1:2109989/89‑1 (MQ=17)
                                                                               aGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCAcagca          <  1:2591126/93‑1 (MQ=255)
                                                                               aGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCAcagca          >  2:8566838/1‑93 (MQ=255)
                                                                                gTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCAcagcag         >  2:4134789/1‑93 (MQ=255)
                                                                                   ccgccgGGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCaa      <  2:2433976/93‑1 (MQ=255)
                                                                                     gccgcggccgcaGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAata    <  2:4527644/93‑1 (MQ=255)
                                                                                       cgcggccgcagTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATc  >  2:5537380/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATC  >  NC_000913/934641‑934820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCA  >  NC_000913/934639‑934824
                                                                                            |                                                                                             
GTTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGGAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGG                                                                                              >  SRR4302541.975320/1‑94 (MQ=60)
GTTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGG                                                                                              >  SRR4302541.6598821/1‑94 (MQ=60)
GTTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACAGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGG                                                                                              <  SRR4302541.8142477/94‑1 (MQ=60)
 TTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGC                                                                                             >  SRR4302541.6711281/1‑94 (MQ=60)
  TTACGCCAATTGTTGACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCC                                                                                            <  SRR4302541.3588297/94‑1 (MQ=60)
  TTACGCCAATTGTTGACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCC                                                                                            <  SRR4302541.3588299/94‑1 (MQ=60)
   TACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCG                                                                                           <  SRR4302541.3885418/94‑1 (MQ=60)
   TACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCG                                                                                           >  SRR4302541.4259675/1‑94 (MQ=60)
   TACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCG                                                                                           <  SRR4302541.2532569/94‑1 (MQ=60)
    ACGCCAATTGTTGACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGC                                                                                          <  SRR4302541.396165/94‑1 (MQ=60)
       CCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTGCGCCGCGGCCGCAGT                                                                                       >  SRR4302541.4650900/1‑94 (MQ=60)
        CAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTA                                                                                      >  SRR4302541.4010999/1‑94 (MQ=60)
         AATTGTTGACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTAT                                                                                     <  SRR4302541.8185468/94‑1 (MQ=60)
         AATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTAT                                                                                     <  SRR4302541.2832120/94‑1 (MQ=60)
          ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATC                                                                                    >  SRR4302541.8781959/1‑94 (MQ=60)
          ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATC                                                                                    <  SRR4302541.5213395/94‑1 (MQ=60)
          ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATC                                                                                    <  SRR4302541.8258968/94‑1 (MQ=60)
           TTGTTGACCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCA                                                                                   <  SRR4302541.757991/94‑1 (MQ=60)
           TTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCA                                                                                   <  SRR4302541.7855455/94‑1 (MQ=60)
           TTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCA                                                                                   <  SRR4302541.1814655/94‑1 (MQ=60)
            TGTTGAACCTTGACACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAG                                                                                  <  SRR4302541.6749609/94‑1 (MQ=57)
            TGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAG                                                                                  >  SRR4302541.8412889/1‑94 (MQ=60)
             ggtaacCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGC                                                                                 <  SRR4302541.1510507/88‑1 (MQ=60)
             TTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGC                                                                                 <  SRR4302541.7363128/94‑1 (MQ=60)
             GTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGC                                                                                 <  SRR4302541.1510508/94‑1 (MQ=60)
              TTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCA                                                                                <  SRR4302541.3540220/94‑1 (MQ=60)
               TGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAG                                                                               <  SRR4302541.7473604/94‑1 (MQ=60)
               TGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAG                                                                               >  SRR4302541.605842/1‑94 (MQ=60)
               TGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAC                                                                               >  SRR4302541.7413952/1‑94 (MQ=60)
                 AACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCC                                                                             >  SRR4302541.5666751/1‑94 (MQ=60)
                 AACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCC                                                                             >  SRR4302541.3288225/1‑94 (MQ=60)
                   CCTGTACAGCAGCCGCAACAACCGGTTGCACAGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCGCCCAC                                                                           >  SRR4302541.5531256/1‑94 (MQ=60)
                    CTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACA                                                                          >  SRR4302541.7842507/1‑94 (MQ=60)
                    CTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTACGCCGCGGCCGCAGTATCAGCAGCCACA                                                                          >  SRR4302541.8040861/1‑94 (MQ=60)
                     TGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAA                                                                         >  SRR4302541.5087652/1‑94 (MQ=60)
                     TGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAA                                                                         >  SRR4302541.8458155/1‑94 (MQ=60)
                      GTACAGCAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAAC                                                                        <  SRR4302541.2381296/94‑1 (MQ=60)
                      GTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAAC                                                                        <  SRR4302541.1134810/94‑1 (MQ=60)
                        aaggcacCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAG                                                                      <  SRR4302541.8019092/87‑1 (MQ=60)
                        ACAGCACCCGCAACACCCGTTTGCACAGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAG                                                                      <  SRR4302541.299127/94‑1 (MQ=49)
                        AAAGCAGCCGCAACAACCGGTTTCACGGAAGCATCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAG                                                                      <  SRR4302541.3311255/94‑1 (MQ=52)
                         CAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGC                                                                     >  SRR4302541.6444766/1‑94 (MQ=60)
                         CAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGC                                                                     >  SRR4302541.8223123/1‑94 (MQ=60)
                          AGCGGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCC                                                                    <  SRR4302541.4307619/94‑1 (MQ=60)
                          AGCAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCC                                                                    <  SRR4302541.8202007/94‑1 (MQ=60)
                           GCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCG                                                                   >  SRR4302541.3489474/1‑94 (MQ=60)
                             cgcccCAACACCCGGTTGCCCCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGT                                                                 <  SRR4302541.5069621/89‑1 (MQ=54)
                             AGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGT                                                                 >  SRR4302541.3231880/1‑94 (MQ=60)
                               cgacaccacccgctcgccccgcagtgccattgtccgcacccacaacaccaAGTTCCGCCGCGGCAGCAGTATCAGCAGCCACAACAGCCGGTTG                                                               <  SRR4302541.7809995/44‑1 (MQ=22)
                               CCGCAACAACGGGTTGCACCGCAGCAGAAATATCACCAGCCGCAACAACCAGTCCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTG                                                               <  SRR4302541.3366546/94‑1 (MQ=60)
                                CGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGC                                                              >  SRR4302541.514454/1‑94 (MQ=60)
                                 GCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGTCGCAGTATCAGCAGCCACAACAGCCGGTTGCG                                                             >  SRR4302541.1943607/1‑94 (MQ=60)
                                 GCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCG                                                             >  SRR4302541.4209230/1‑94 (MQ=60)
                                  CAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGC                                                            >  SRR4302541.1215353/1‑94 (MQ=60)
                                   AACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGTCCGCAGTATCAGACGACACAACCGCCGGTTGCGCC                                                           >  SRR4302541.2228822/1‑94 (MQ=54)
                                     CAACCGGTTGCCCCGCAGCACCAATATCAGCAGCCGCAACACCCAGTCCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGC                                                         <  SRR4302541.8263851/94‑1 (MQ=57)
                                     CAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGAGCAGC                                                         >  SRR4302541.5014672/1‑94 (MQ=60)
                                     AAACCGGTTGCACCGCAGCAGCAATACCACCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGC                                                         <  SRR4302541.1343905/94‑1 (MQ=60)
                                      AACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCCGCAGCCACAACAGCCGGTTGCGCCGCA                                                        >  SRR4302541.2296573/1‑94 (MQ=60)
                                         CGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCC                                                     >  SRR4302541.909865/1‑94 (MQ=60)
                                          GGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCA                                                    <  SRR4302541.2573023/94‑1 (MQ=60)
                                           gtgccccggaAGCAGAAATATCACCAGCCGCACCAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCAC                                                   <  SRR4302541.436131/84‑1 (MQ=60)
                                           GTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCAC                                                   <  SRR4302541.7247637/94‑1 (MQ=60)
                                           GTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCAC                                                   >  SRR4302541.6528037/1‑94 (MQ=60)
                                            TTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACA                                                  >  SRR4302541.8155498/1‑94 (MQ=60)
                                            TTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACA                                                  >  SRR4302541.6232625/1‑94 (MQ=60)
                                             TGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAA                                                 >  SRR4302541.7678468/1‑94 (MQ=60)
                                                CCCGCAGCAGCAATATCAGCAGCCGCAACACCCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATAT                                              <  SRR4302541.6576032/94‑1 (MQ=60)
                                                ACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATAT                                              >  SRR4302541.3960849/1‑94 (MQ=60)
                                                  cgtgcaagatatacgAGCAGCCACAACAACCTGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCA                                            <  SRR4302541.5640758/79‑1 (MQ=60)
                                                  CGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCA                                            >  SRR4302541.4432324/1‑94 (MQ=60)
                                                  CGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCA                                            >  SRR4302541.1784953/1‑94 (MQ=60)
                                                   caagcaaaaatagCAGCAGCCGCAACACCAAGTTCCGCCGAGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAG                                           <  SRR4302541.4866935/81‑1 (MQ=60)
                                                   GCAGCAGCAATACCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAG                                           <  SRR4302541.3079852/94‑1 (MQ=60)
                                                     agcgcaaacaccagcgGCCGCAACAACAAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCA                                         <  SRR4302541.1822347/78‑1 (MQ=60)
                                                     AGCAGCAACATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCA                                         <  SRR4302541.660600/94‑1 (MQ=60)
                                                       CAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGC                                       >  SRR4302541.7309029/1‑94 (MQ=60)
                                                       CAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGC                                       >  SRR4302541.8859541/1‑94 (MQ=60)
                                                          CAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGC                                    >  SRR4302541.8512266/1‑94 (MQ=60)
                                                          CAATATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGC                                    >  SRR4302541.8157027/1‑94 (MQ=60)
                                                           AATATCAGCAGCCGCAACAACCAGTTCCGCCGCGCCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCA                                   <  SRR4302541.3087333/94‑1 (MQ=60)
                                                             tacaAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAAC                                 <  SRR4302541.4813800/90‑1 (MQ=60)
                                                             TATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAAC                                 >  SRR4302541.8863105/1‑94 (MQ=60)
                                                             TATCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAAC                                 >  SRR4302541.8269272/1‑94 (MQ=60)
                                                               TCAGCAGCCGCAACAACCAGTTCCGCCGCGGCCTCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAA                               <  SRR4302541.8611132/94‑1 (MQ=60)
                                                                CAGCAGCCGCAACAACCAGTCCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAAC                              <  SRR4302541.244549/94‑1 (MQ=60)
                                                                 AGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACC                             <  SRR4302541.5503461/94‑1 (MQ=60)
                                                                 AGCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACC                             <  SRR4302541.5365256/94‑1 (MQ=60)
                                                                  GCAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCG                            >  SRR4302541.2615746/1‑94 (MQ=60)
                                                                   CAGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCCCAGCCACAATATCAGCAGCCGCAACAACCGG                           >  SRR4302541.7306238/1‑94 (MQ=60)
                                                                    AGCCGCAACACCCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGT                          <  SRR4302541.8480625/94‑1 (MQ=60)
                                                                    AGCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCGGCAACAACCGGT                          >  SRR4302541.2244856/1‑94 (MQ=60)
                                                                    AGCCGCAACAACCAGTTCCGCCGCGGCCGAAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGT                          <  SRR4302541.8655655/94‑1 (MQ=60)
                                                                     gccggtcCAACCAGTTCCGCCGCGGCCGCGGTATCAGCAGCCCCAGCAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTT                         <  SRR4302541.1476782/87‑1 (MQ=60)
                                                                     GCCGCAACACCCAGTCCCGCCGCGGCCGCAGTATCACCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTT                         <  SRR4302541.6329497/94‑1 (MQ=60)
                                                                     GCCGCAACAACCATTTCCGCCGCGGCCGCATTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTT                         <  SRR4302541.1476783/94‑1 (MQ=60)
                                                                     GCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCGGCAGCCACAATATCAGCAGCCGCAACAACCGGTG                         >  SRR4302541.42963/1‑94 (MQ=60)
                                                                     GCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTT                         <  SRR4302541.1804343/94‑1 (MQ=60)
                                                                     GCCGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTT                         <  SRR4302541.4815844/94‑1 (MQ=60)
                                                                       CGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGC                       >  SRR4302541.1173264/1‑94 (MQ=60)
                                                                       CGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGC                       >  SRR4302541.7460354/1‑94 (MQ=60)
                                                                       CGCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGC                       >  SRR4302541.7104379/1‑94 (MQ=60)
                                                                        GCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGgc                      >  SRR4302541.6909194/1‑92 (MQ=60)
                                                                        GCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCG                      >  SRR4302541.5436831/1‑94 (MQ=60)
                                                                        GCAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCG                      >  SRR4302541.5436832/1‑94 (MQ=60)
                                                                         CAACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGC                     >  SRR4302541.1830221/1‑94 (MQ=60)
                                                                          AACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCACCAACCGGTTGCGCA                    >  SRR4302541.5191273/1‑94 (MQ=60)
                                                                          AACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCC                    >  SRR4302541.4524816/1‑94 (MQ=60)
                                                                           ACAACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCA                   >  SRR4302541.7630527/1‑94 (MQ=60)
                                                                             accaagtcCCGCCGCGGCCGCATTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACA                 <  SRR4302541.8101118/86‑1 (MQ=60)
                                                                             AACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACA                 >  SRR4302541.7255745/1‑94 (MQ=60)
                                                                             AACCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACA                 <  SRR4302541.7530662/94‑1 (MQ=60)
                                                                             AACCAGTTCCCCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACA                 <  SRR4302541.6343742/94‑1 (MQ=60)
                                                                               CCAGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGC               >  SRR4302541.6272923/1‑94 (MQ=60)
                                                                                CAGTCCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCA              <  SRR4302541.4274267/94‑1 (MQ=60)
                                                                                CAGTCCCGCCGCGGCCGCAGTATCACCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCA              <  SRR4302541.7004930/94‑1 (MQ=60)
                                                                                 cgccCCGCCGCGGCCGCAATAGCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAG             <  SRR4302541.2130428/90‑1 (MQ=60)
                                                                                 AGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAG             <  SRR4302541.2617091/94‑1 (MQ=60)
                                                                                 AGTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGACGCAACAACCGGTTGCGACACAGCAG             >  SRR4302541.8917623/1‑94 (MQ=60)
                                                                                  GTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGC            >  SRR4302541.2806622/1‑94 (MQ=60)
                                                                                  GTTCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCACCAGCCGCAACAACCGGCTGCGCCACAGCAGC            >  SRR4302541.1228162/1‑94 (MQ=60)
                                                                                  GTTCCGCCGCGGCCACAGTATCAGCACCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGC            >  SRR4302541.8043896/1‑94 (MQ=60)
                                                                                    TCCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAA          <  SRR4302541.1717845/94‑1 (MQ=60)
                                                                                     CCGCCGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAAT         <  SRR4302541.8434184/94‑1 (MQ=60)
                                                                                         CGCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCA     >  SRR4302541.4467870/1‑94 (MQ=60)
                                                                                          GCGGCGGCAGTATCAGCAGCCACAACAGCCGGTGCCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAG    <  SRR4302541.7860041/94‑1 (MQ=60)
                                                                                          GCGGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAG    <  SRR4302541.8169922/94‑1 (MQ=60)
                                                                                            GGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCA  >  SRR4302541.6429109/1‑94 (MQ=60)
                                                                                            |                                                                                             
GTTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAACAACCGGTTGCGCCACAGCAGCAATATCAGCA  >  NC_000913/934639‑934824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: