Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	946,689	A→G	T144A (ACG→GCG)	b0899 →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	946,689	0	A	G	99.6%	851.6 / ‑4.8	252	T144A (ACG→GCG)	b0899	putative transport
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (123/128); total (123/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 268 total aligned reads displayed.

CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTG  >  NC_000913/946599‑946778

ctctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACAcgcg                                                                                         <  1:2194282/93‑1 (MQ=255)

 tctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAGGGGCGGGGTCAGTACCTGGATTAAACACGCGa                                                                                        <  1:7558981/93‑1 (MQ=255)

 tctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGa                                                                                        <  1:6027814/93‑1 (MQ=255)

 tctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGa                                                                                        >  1:5210283/1‑93 (MQ=255)

  ctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGAt                                                                                       <  2:2161631/93‑1 (MQ=255)

   tATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATg                                                                                      >  1:4244926/1‑93 (MQ=255)

   tATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATg                                                                                      >  2:7273368/1‑93 (MQ=255)

   tATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACAAGCGATg                                                                                      >  1:4618621/1‑93 (MQ=255)

    aTTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATgg                                                                                     <  2:1569792/93‑1 (MQ=255)

    aTTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATgg                                                                                     >  1:3200978/1‑93 (MQ=255)

       tCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTGAGTACCTGGATTAAACACGCGAGGGGAc                                                                                  >  1:7002787/1‑93 (MQ=255)

       tCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAc                                                                                  <  2:1396734/93‑1 (MQ=255)

       tCACAACTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAc                                                                                  <  1:4466979/93‑1 (MQ=255)

         acacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                >  1:6337391/1‑93 (MQ=255)

         acacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                <  2:6515202/93‑1 (MQ=255)

         acacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                >  1:3936102/1‑93 (MQ=255)

         acacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                >  2:186357/1‑93 (MQ=255)

          cacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCg                                                                               >  1:4351522/1‑93 (MQ=255)

          cacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCAGGGTCATTACCTGGATTAAACACGCCATGGGACCCg                                                                               <  2:4148698/93‑1 (MQ=255)

           acCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCgg                                                                              <  1:7435316/93‑1 (MQ=255)

            ccTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGa                                                                             <  1:7745698/93‑1 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            <  2:8302919/93‑1 (MQ=255)

               tATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTg                                                                          >  1:6163308/1‑93 (MQ=255)

                 tGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGc                                                                        <  2:2452942/93‑1 (MQ=255)

                   cgcTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGGCAGTAACTGGATTAAACACGGGATGGGACCCGGACTGTCtt                                                                      >  1:2334476/1‑93 (MQ=255)

                    gcTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCtga                                                                     >  1:1591621/1‑91 (MQ=255)

                    gcTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTa                                                                     >  2:8448804/1‑93 (MQ=255)

                     cTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAt                                                                    >  1:6997526/1‑93 (MQ=255)

                      tAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGTGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtc                                                                   >  1:4399113/1‑93 (MQ=255)

                       aaTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtct                                                                  <  1:3999108/93‑1 (MQ=255)

                       aaTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtct                                                                  >  2:7898583/1‑93 (MQ=255)

                       aaTTGTTCGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtct                                                                  <  1:2893833/93‑1 (MQ=255)

                        aTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtctc                                                                 <  1:8755652/93‑1 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               >  2:2083027/1‑93 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               <  1:2721120/93‑1 (MQ=255)

                           gttgGTGAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgcc                                                              <  1:1729708/93‑1 (MQ=255)

                             tgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgccgc                                                            >  1:6686867/1‑93 (MQ=255)

                              ggTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCcgcg                                                           <  1:1153076/93‑1 (MQ=255)

                              ggTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCcgcg                                                           >  2:6717706/1‑93 (MQ=255)

                                 cAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTgg                                                        <  1:7240260/93‑1 (MQ=255)

                                  aGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTggc                                                       >  2:2228962/1‑92 (MQ=255)

                                  aGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGa                                                       <  2:5920566/93‑1 (MQ=255)

                                   gTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAc                                                      >  1:4723348/1‑93 (MQ=255)

                                    ttAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAcc                                                     >  2:4049712/1‑93 (MQ=255)

                                    ttAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAcc                                                     <  2:3188900/93‑1 (MQ=255)

                                     tAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCt                                                    >  1:8543199/1‑93 (MQ=255)

                                      aGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTa                                                   >  2:1888774/1‑93 (MQ=255)

                                       ggCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTAc                                                  <  1:5691686/93‑1 (MQ=255)

                                         cTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTg                                                <  2:455449/93‑1 (MQ=255)

                                         cTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTg                                                <  1:260967/93‑1 (MQ=255)

                                          tCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTgg                                               <  2:1032237/93‑1 (MQ=255)

                                            gACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggt                                             <  1:4924845/93‑1 (MQ=255)

                                            gACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggt                                             <  2:5416931/93‑1 (MQ=255)

                                            gACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggt                                             <  2:2599169/93‑1 (MQ=255)

                                              ccTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggtgg                                           <  1:5292927/93‑1 (MQ=255)

                                               cTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggtggt                                          <  2:2988895/93‑1 (MQ=255)

                                                ttCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGgtggtg                                         >  2:4957363/1‑93 (MQ=255)

                                                ttCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGgtggtg                                         >  2:3811891/1‑93 (MQ=255)

                                                 tCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGc                                        >  2:5677061/1‑93 (MQ=255)

                                                 tCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGc                                        >  2:5174914/1‑93 (MQ=255)

                                                  cAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCCACTGGGTGGTGCa                                       >  2:3394117/1‑93 (MQ=255)

                                                   aaaGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCat                                      <  2:8211691/93‑1 (MQ=255)

                                                   aaaGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCat                                      >  2:936619/1‑93 (MQ=255)

                                                   aaaGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCat                                      >  2:1515160/1‑93 (MQ=255)

                                                    aaGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCata                                     >  1:5077184/1‑93 (MQ=255)

                                                    aaGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCata                                     >  2:3619481/1‑93 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  1:5780368/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    >  2:4744582/1‑93 (MQ=255)

                                                      gATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATAtt                                   <  1:6599399/93‑1 (MQ=255)

                                                           gAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTa                              <  2:1901787/93‑1 (MQ=255)

                                                           gAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTa                              <  1:3855266/93‑1 (MQ=255)

                                                            aaGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTAt                             >  1:3584586/1‑93 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  2:6548141/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  2:3508972/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            >  2:4932937/1‑93 (MQ=255)

                                                              gggCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCt                           >  2:5387178/1‑93 (MQ=255)

                                                              gggCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCt                           <  2:7967347/93‑1 (MQ=255)

                                                               ggCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTg                          >  1:6462602/1‑93 (MQ=255)

                                                               ggCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTg                          <  2:8531539/93‑1 (MQ=255)

                                                               ggCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTg                          <  1:7392078/93‑1 (MQ=255)

                                                                gCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTgg                         <  2:2002920/93‑1 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  1:4968985/1‑93 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  2:6859653/1‑93 (MQ=255)

                                                                      tCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACaaa                   >  2:6347980/1‑93 (MQ=255)

                                                                      tCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACaaa                   <  1:2999407/93‑1 (MQ=255)

                                                                         gTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAc                <  2:1560370/93‑1 (MQ=255)

                                                                           aCCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAccc              >  2:1354403/1‑93 (MQ=255)

                                                                            ccTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAcccc             <  1:7513374/93‑1 (MQ=255)

                                                                             cTGGATTAAACACGCGTTGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGTTGCACATTCCCTATCTGGCACAAAAACCCCa            >  2:2784931/1‑93 (MQ=255)

                                                                              tGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAg           <  1:1078019/93‑1 (MQ=255)

                                                                              tGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAg           <  1:3237469/93‑1 (MQ=255)

                                                                               ggATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAgg          >  1:5359/1‑93 (MQ=255)

                                                                               ggATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAgg          >  1:7302845/1‑93 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  1:1033786/1‑93 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  1:588605/1‑93 (MQ=255)

                                                                                   tAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAt      <  1:1882787/93‑1 (MQ=255)

                                                                                   tAAACACGCGATGGGACCCGGACGGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAt      <  2:5977893/93‑1 (MQ=255)

                                                                                    aaaCACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAtt     <  1:8299037/93‑1 (MQ=255)

                                                                                     aaCACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAACCCAGGCAATTc    >  2:7573415/1‑93 (MQ=255)

                                                                                      acacgcGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTCGGCGGTGCATCTTCCCTATCTGGCACAAAAACCCCACGCAATTCt   >  1:6288951/1‑93 (MQ=255)

CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTG  >  NC_000913/946599‑946778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCT  >  NC_000913/946596‑946777

GCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACG                                                                                          <  SRR4302541.43800/94‑1 (MQ=60)

 CACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGC                                                                                         >  SRR4302541.1226063/1‑94 (MQ=60)

  ACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTCAACACGCG                                                                                        >  SRR4302541.8430514/1‑94 (MQ=60)

  ACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCG                                                                                        >  SRR4302541.5233105/1‑94 (MQ=60)

  ACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCG                                                                                        <  SRR4302541.3248806/94‑1 (MQ=60)

  ACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCG                                                                                        >  SRR4302541.2023859/1‑94 (MQ=60)

  ACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCG                                                                                        <  SRR4302541.1837443/94‑1 (MQ=60)

   CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGA                                                                                       >  SRR4302541.5263963/1‑94 (MQ=60)

   CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGA                                                                                       <  SRR4302541.2215511/94‑1 (MQ=60)

    TCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAGGGGCGGGGTCAGTACCTGGATTAAACACGCGAT                                                                                      <  SRR4302541.7639764/94‑1 (MQ=60)

    TCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGAT                                                                                      <  SRR4302541.5622446/94‑1 (MQ=60)

    TCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGAT                                                                                      <  SRR4302541.6089568/94‑1 (MQ=60)

     CTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATG                                                                                     <  SRR4302541.6752348/94‑1 (MQ=60)

     CTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATG                                                                                     >  SRR4302541.4287307/1‑94 (MQ=60)

     CTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACAAGCGATG                                                                                     >  SRR4302541.4665568/1‑94 (MQ=60)

      TATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGG                                                                                    >  SRR4302541.6391743/1‑94 (MQ=60)

      TATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGG                                                                                    >  SRR4302541.3233317/1‑94 (MQ=60)

      TATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGG                                                                                    <  SRR4302541.6102220/94‑1 (MQ=60)

         TTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTGAGTACCTGGATTAAACACGCGAGGGGAC                                                                                 >  SRR4302541.7077298/1‑94 (MQ=60)

         TTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAC                                                                                 >  SRR4302541.4669668/1‑94 (MQ=60)

         TTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAC                                                                                 >  SRR4302541.7077297/1‑94 (MQ=60)

          TCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACC                                                                                >  SRR4302541.435365/1‑94 (MQ=60)

          TCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACC                                                                                <  SRR4302541.5925622/94‑1 (MQ=60)

          TCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACC                                                                                <  SRR4302541.3426127/94‑1 (MQ=60)

          TCACAACTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACC                                                                                <  SRR4302541.4511973/94‑1 (MQ=60)

           CACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCC                                                                               >  SRR4302541.3975288/1‑94 (MQ=60)

           CACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCC                                                                               >  SRR4302541.6402179/1‑94 (MQ=60)

            ACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCG                                                                              >  SRR4302541.5994988/1‑94 (MQ=60)

            ACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCG                                                                              >  SRR4302541.4394971/1‑94 (MQ=60)

             CACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGG                                                                             <  SRR4302541.2945133/94‑1 (MQ=60)

              ACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGA                                                                            <  SRR4302541.7514796/94‑1 (MQ=60)

              ACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGA                                                                            >  SRR4302541.4408627/1‑94 (MQ=60)

               CCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAC                                                                           <  SRR4302541.7828116/94‑1 (MQ=60)

               CCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAC                                                                           >  SRR4302541.6569793/1‑94 (MQ=60)

                CTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACT                                                                          <  SRR4302541.7483106/94‑1 (MQ=60)

                CTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACT                                                                          <  SRR4302541.2771828/94‑1 (MQ=60)

                 TTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTG                                                                         <  SRR4302541.1125891/94‑1 (MQ=60)

                 TTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTG                                                                         >  SRR4302541.6226290/1‑94 (MQ=60)

                  TATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGG                                                                        <  SRR4302541.2634304/94‑1 (MQ=60)

                  TATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGG                                                                        <  SRR4302541.8491574/94‑1 (MQ=60)

                  TATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGG                                                                        >  SRR4302541.3825628/1‑94 (MQ=60)

                    TGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCT                                                                      >  SRR4302541.6378094/1‑94 (MQ=60)

                     GCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGGCAGTAACTGGATTAAACACGGGATGGGACCCGGACTGTCTT                                                                     >  SRR4302541.2357146/1‑94 (MQ=60)

                      CGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTA                                                                    >  SRR4302541.6502283/1‑94 (MQ=60)

                      CGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTA                                                                    >  SRR4302541.1222842/1‑94 (MQ=60)

                      CGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTGA                                                                    >  SRR4302541.1607467/1‑94 (MQ=60)

                       GCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAT                                                                   >  SRR4302541.7072004/1‑94 (MQ=60)

                       GCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAT                                                                   <  SRR4302541.4147184/94‑1 (MQ=60)

                        CTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGTGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATC                                                                  >  SRR4302541.4443118/1‑94 (MQ=60)

                        CTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATC                                                                  >  SRR4302541.4819478/1‑94 (MQ=60)

                          AATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTC                                                                <  SRR4302541.4038699/94‑1 (MQ=60)

                          AATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTC                                                                <  SRR4302541.7429504/94‑1 (MQ=60)

                          AATTGTTCGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTC                                                                <  SRR4302541.2922916/94‑1 (MQ=60)

                           ATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCG                                                               <  SRR4302541.8849314/94‑1 (MQ=60)

                             TGTTGGTCAGTTAGTCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCC                                                             <  SRR4302541.5001611/94‑1 (MQ=60)

                             TGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCC                                                             <  SRR4302541.5001610/94‑1 (MQ=60)

                             TGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCC                                                             <  SRR4302541.2748285/94‑1 (MQ=60)

                              GTTGGTGAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCG                                                            <  SRR4302541.1746599/94‑1 (MQ=60)

                               TTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGC                                                           >  SRR4302541.6756199/1‑94 (MQ=60)

                                TGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACTTGGATTAAACACCCGATGGGACCCGGACTGGCTTGTCTCGCCGCG                                                          >  SRR4302541.2722778/1‑94 (MQ=60)

                                 GGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGT                                                         <  SRR4302541.1164101/94‑1 (MQ=60)

                                    CAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGA                                                      >  SRR4302541.4775861/1‑94 (MQ=60)

                                    CAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGA                                                      <  SRR4302541.7317472/94‑1 (MQ=60)

                                     AGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAC                                                     >  SRR4302541.7539601/1‑94 (MQ=60)

                                     AGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAC                                                     <  SRR4302541.5392820/94‑1 (MQ=60)

                                     AGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAC                                                     >  SRR4302541.4771523/1‑94 (MQ=60)

                                     AGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAC                                                     >  SRR4302541.1159334/1‑94 (MQ=60)

                                      GTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACC                                                    <  SRR4302541.1721784/94‑1 (MQ=60)

                                      GTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACC                                                    >  SRR4302541.5788529/1‑94 (MQ=60)

                                      GTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACC                                                    <  SRR4302541.5758160/94‑1 (MQ=60)

                                      GTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACC                                                    <  SRR4302541.7590286/94‑1 (MQ=60)

                                       TTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTGCCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCT                                                   <  SRR4302541.4445348/94‑1 (MQ=60)

                                       TTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCT                                                   >  SRR4302541.8635036/1‑94 (MQ=60)

                                        TAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTGATCTCGCCGCGGGGACCTA                                                  >  SRR4302541.3239005/1‑94 (MQ=60)

                                         AGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTAC                                                 <  SRR4302541.2678921/94‑1 (MQ=60)

                                          GGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACT                                                <  SRR4302541.5749745/94‑1 (MQ=60)

                                           GCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTG                                               <  SRR4302541.1483441/94‑1 (MQ=60)

                                           GCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGGGGACCTACTG                                               >  SRR4302541.2548824/1‑94 (MQ=60)

                                            CTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGG                                              <  SRR4302541.263096/94‑1 (MQ=60)

                                              CGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGT                                            <  SRR4302541.3117773/94‑1 (MQ=60)

                                               GACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTG                                           <  SRR4302541.4975089/94‑1 (MQ=60)

                                               GACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTG                                           <  SRR4302541.7692277/94‑1 (MQ=60)

                                                GCCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGG                                          <  SRR4302541.5141729/94‑1 (MQ=60)

                                                ACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGG                                          <  SRR4302541.4669873/94‑1 (MQ=60)

                                                 CCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGT                                         <  SRR4302541.5347414/94‑1 (MQ=60)

                                                  CTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTG                                        >  SRR4302541.7342653/1‑94 (MQ=60)

                                                  CTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTG                                        >  SRR4302541.2056868/1‑94 (MQ=60)

                                                   TTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGC                                       <  SRR4302541.7321600/94‑1 (MQ=60)

                                                     CAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCAT                                     >  SRR4302541.5963658/1‑94 (MQ=60)

                                                      AAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATA                                    >  SRR4302541.5129350/1‑94 (MQ=60)

                                                       AAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGCCTGGCTTATATCGCCGCCTGGACCTACTGGGTGGTGTATAT                                   >  SRR4302541.5060335/1‑94 (MQ=60)

                                                        AGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATT                                  <  SRR4302541.5839139/94‑1 (MQ=60)

                                                        AGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATT                                  <  SRR4302541.3978012/94‑1 (MQ=60)

                                                         GATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTC                                 <  SRR4302541.6666988/94‑1 (MQ=60)

                                                         GATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTC                                 <  SRR4302541.6666989/94‑1 (MQ=60)

                                                         GATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTC                                 <  SRR4302541.5653067/94‑1 (MQ=60)

                                                         GATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTC                                 <  SRR4302541.4029871/94‑1 (MQ=60)

                                                         GATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTC                                 >  SRR4302541.8168972/1‑94 (MQ=60)

                                                          ATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCC                                >  SRR4302541.7625877/1‑94 (MQ=60)

                                                          ATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCC                                >  SRR4302541.8885123/1‑94 (MQ=60)

                                                            GGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCT                              <  SRR4302541.4410321/94‑1 (MQ=60)

                                                             GGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTA                             >  SRR4302541.5308612/1‑94 (MQ=60)

                                                              GAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTAT                            <  SRR4302541.3894058/94‑1 (MQ=60)

                                                              GAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTAT                            <  SRR4302541.4483700/94‑1 (MQ=60)

                                                              GAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTAT                            >  SRR4302541.3620917/1‑94 (MQ=60)

                                                              GAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTAT                            <  SRR4302541.6145628/94‑1 (MQ=60)

                                                                AGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCT                          >  SRR4302541.6460816/1‑94 (MQ=60)

                                                                AGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCT                          <  SRR4302541.362863/94‑1 (MQ=60)

                                                                AGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCT                          <  SRR4302541.5092572/94‑1 (MQ=60)

                                                                AGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGG‑GGGGGATATTCCCTATCTG                         >  SRR4302541.6899639/1‑94 (MQ=60)

                                                                AGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAGCTCGCCGCGTGGTCCTTTTGGGGGGTGCATATTCCCTATCT                          >  SRR4302541.7920328/1‑94 (MQ=60)

                                                                 GGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTG                         >  SRR4302541.6528461/1‑94 (MQ=60)

                                                                  GGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGG                        <  SRR4302541.7471125/94‑1 (MQ=60)

                                                                  GGCGGGGGCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGG                        <  SRR4302541.3247924/94‑1 (MQ=60)

                                                                     GGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCAC                     <  SRR4302541.865468/94‑1 (MQ=60)

                                                                     GGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCAC                     >  SRR4302541.5019898/1‑94 (MQ=60)

                                                                     GGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCAC                     >  SRR4302541.7150534/1‑94 (MQ=60)

                                                                         TCAGTATCTGGATGAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAA                 <  SRR4302541.3029682/94‑1 (MQ=60)

                                                                         TCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAA                 <  SRR4302541.3029683/94‑1 (MQ=60)

                                                                          CAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAA                <  SRR4302541.1801371/94‑1 (MQ=60)

                                                                          CAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAA                <  SRR4302541.6346497/94‑1 (MQ=60)

                                                                           AGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAC               >  SRR4302541.4062413/1‑94 (MQ=60)

                                                                             GACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCC             <  SRR4302541.683601/94‑1 (MQ=60)

                                                                              ACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCC            <  SRR4302541.1889555/94‑1 (MQ=60)

                                                                              ACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCC            >  SRR4302541.4565130/1‑94 (MQ=60)

                                                                              ACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCC            <  SRR4302541.3952728/94‑1 (MQ=60)

                                                                               CCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCA           <  SRR4302541.7593673/94‑1 (MQ=60)

                                                                                CTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAG          <  SRR4302541.7496562/94‑1 (MQ=60)

                                                                                CTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAG          <  SRR4302541.7650684/94‑1 (MQ=60)

                                                                                 TGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGG         <  SRR4302541.3404787/94‑1 (MQ=60)

                                                                                 TGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGG         <  SRR4302541.1088064/94‑1 (MQ=60)

                                                                                 TGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGG         >  SRR4302541.7380802/1‑94 (MQ=60)

                                                                                 TGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGG         <  SRR4302541.3130653/94‑1 (MQ=60)

                                                                                 TGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGG         <  SRR4302541.3270160/94‑1 (MQ=60)

                                                                                 TGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGG         >  SRR4302541.5402/1‑94 (MQ=60)

                                                                                  GGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGA        >  SRR4302541.5293399/1‑94 (MQ=60)

                                                                                  GGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGA        >  SRR4302541.2302836/1‑94 (MQ=60)

                                                                                    ATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAA      >  SRR4302541.594148/1‑94 (MQ=60)

                                                                                    ATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAA      >  SRR4302541.1043329/1‑94 (MQ=60)

                                                                                      TAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATT    <  SRR4302541.1900874/94‑1 (MQ=60)

                                                                                      TAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATT    <  SRR4302541.7202970/94‑1 (MQ=60)

                                                                                       AAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTC   <  SRR4302541.8389278/94‑1 (MQ=60)

                                                                                        AACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCT  <  SRR4302541.1058635/94‑1 (MQ=60)

                                                                                        AACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTCGGCGGTGCATCTTCCCTATCTGGCACAAAAACCCCACGCAATTCT  >  SRR4302541.6353199/1‑94 (MQ=60)

GCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCT  >  NC_000913/946596‑946777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`