Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 961,423 A→G D69G (GAC→GGC)  rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913961,4230AG100.0% 907.4 / NA 260D69G (GAC→GGC) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (135/125);  total (135/125)

Only 100 of 266 total aligned reads displayed.

CGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGACGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTG  >  NC_000913/961334‑961511
                                                                                         |                                                                                        
cGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGt                                                                                       >  2:1568890/1‑93 (MQ=255)
cGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGt                                                                                       <  1:2542764/93‑1 (MQ=255)
 gCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGtt                                                                                      <  1:5954907/93‑1 (MQ=255)
 gCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGtt                                                                                      >  1:7221747/1‑93 (MQ=255)
  cTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTg                                                                                     >  1:6862190/1‑93 (MQ=255)
   tGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGc                                                                                    <  2:6349268/93‑1 (MQ=255)
     gTCTGAGATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctc                                                                                  <  2:6057048/93‑1 (MQ=255)
     gTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctc                                                                                  <  2:3375900/93‑1 (MQ=255)
     gTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctc                                                                                  <  2:1767658/93‑1 (MQ=255)
     gTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctc                                                                                  >  2:4561276/1‑93 (MQ=255)
     gTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctc                                                                                  <  1:8172936/93‑1 (MQ=255)
      tCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctct                                                                                 <  2:1672170/93‑1 (MQ=255)
      tCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctct                                                                                 <  2:703619/93‑1 (MQ=255)
      tCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGCTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGctct                                                                                 >  2:8122198/1‑93 (MQ=255)
            agcTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGc                                                                           <  2:1495740/91‑1 (MQ=255)
              cTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAg                                                                         <  1:8448915/93‑1 (MQ=255)
              cTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAg                                                                         >  2:2616075/1‑93 (MQ=255)
               tGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGt                                                                        <  2:7483977/93‑1 (MQ=255)
                gAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTa                                                                       <  1:4573939/93‑1 (MQ=255)
                   tCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTagaa                                                                    >  1:7564376/1‑93 (MQ=255)
                   tCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTagaa                                                                    <  2:7852222/93‑1 (MQ=255)
                   tCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTagaa                                                                    >  1:5600269/1‑93 (MQ=255)
                     cGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTagaaga                                                                  >  2:127622/1‑93 (MQ=255)
                      gCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGAc                                                                 <  1:5115594/93‑1 (MQ=255)
                       ccATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACg                                                                >  1:6043647/1‑93 (MQ=255)
                        cATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACgg                                                               <  1:6298962/93‑1 (MQ=255)
                        cATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGAAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGCCgg                                                               <  2:5820941/93‑1 (MQ=255)
                         aTCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGc                                                              >  1:6920340/1‑93 (MQ=255)
                         aTCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGc                                                              >  1:1041762/1‑93 (MQ=255)
                         aTCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGc                                                              <  2:8704292/93‑1 (MQ=255)
                          tCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGTAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACggat                                                             >  1:4039592/1‑91 (MQ=255)
                          tCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCt                                                             <  1:2519626/93‑1 (MQ=255)
                           cccGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCtt                                                            <  1:5406608/93‑1 (MQ=255)
                            ccGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTc                                                           <  1:8281772/93‑1 (MQ=255)
                            ccGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTc                                                           >  2:5320055/1‑93 (MQ=255)
                             cGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCg                                                          <  2:5052986/93‑1 (MQ=255)
                              ggCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCgg                                                         <  2:3724851/93‑1 (MQ=255)
                                cTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTg                                                       >  1:7411009/1‑93 (MQ=255)
                                  gAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGaa                                                     >  2:802852/1‑93 (MQ=255)
                                   aGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGaaa                                                    >  2:1839134/1‑93 (MQ=255)
                                    gCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGTTCTGGACGCAGTAGAAGACGGCTTCGGTGaaat                                                   >  2:2156147/1‑92 (MQ=255)
                                      aGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAActc                                                 >  2:4329299/1‑93 (MQ=255)
                                      aGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAActc                                                 <  2:2271929/93‑1 (MQ=255)
                                       gTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCTTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAActct                                                <  2:3847615/93‑1 (MQ=255)
                                       gTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAActct                                                >  1:1811628/1‑93 (MQ=255)
                                        ttCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTctg                                               >  1:5846460/1‑93 (MQ=255)
                                          cAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTctgct                                             <  2:4039272/93‑1 (MQ=255)
                                          cAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTctgct                                             <  2:2373169/93‑1 (MQ=255)
                                            aaaaCGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTctgctgc                                           >  1:1149758/1‑92 (MQ=255)
                                                 gCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGt                                      <  2:6705673/93‑1 (MQ=255)
                                                   ccAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTga                                    >  1:6746233/1‑93 (MQ=255)
                                                      gggCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGaa                                 >  2:3180116/1‑93 (MQ=255)
                                                       ggCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGaaa                                <  1:2842350/93‑1 (MQ=255)
                                                         cGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGc                              >  1:6999471/1‑93 (MQ=255)
                                                          gAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAATCTCTGCTGTCCCGTGAGAAAGCt                             >  2:5964391/1‑93 (MQ=255)
                                                          gAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCTCGTGAGAAAGCt                             >  1:4660340/1‑93 (MQ=255)
                                                          gAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCt                             >  2:3579229/1‑93 (MQ=255)
                                                           aGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTa                            >  1:3469473/1‑93 (MQ=255)
                                                           aGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTa                            <  1:5821387/93‑1 (MQ=255)
                                                           aGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTa                            >  1:6591275/1‑93 (MQ=255)
                                                           aGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTa                            <  2:4600608/93‑1 (MQ=255)
                                                             cTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGTTGTCCCGTGAGAAAGCTaaa                          <  2:7221747/93‑1 (MQ=255)
                                                             cTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTaaa                          <  2:3957677/93‑1 (MQ=255)
                                                              tGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAAc                         <  1:4791391/93‑1 (MQ=255)
                                                                gAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACgc                       >  1:7762280/1‑92 (MQ=255)
                                                                gAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGt                       <  1:3869233/93‑1 (MQ=255)
                                                                gAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGt                       >  2:1215449/1‑93 (MQ=255)
                                                                 aaaTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTc                      <  1:351214/93‑1 (MQ=255)
                                                                 aaaTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTc                      <  2:19526/93‑1 (MQ=255)
                                                                 aaaTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTc                      >  2:6725685/1‑93 (MQ=255)
                                                                  aaTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCa                     <  1:7458438/93‑1 (MQ=255)
                                                                   aTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCAc                    >  1:4334515/1‑93 (MQ=255)
                                                                   aTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCAc                    <  1:6024531/93‑1 (MQ=255)
                                                                   aTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCAc                    >  2:6223354/1‑93 (MQ=255)
                                                                   aTCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCAc                    <  1:8555264/93‑1 (MQ=255)
                                                                       aggtaggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAg                >  2:655068/1‑93 (MQ=255)
                                                                       aggtaggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAg                >  1:6450166/1‑93 (MQ=255)
                                                                        ggtaggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGc               <  1:2351151/93‑1 (MQ=255)
                                                                         gtaggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGcc              <  1:3062519/93‑1 (MQ=255)
                                                                         gtaggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGcc              >  2:2549379/1‑93 (MQ=255)
                                                                          taggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCt             <  1:1561609/93‑1 (MQ=255)
                                                                           aggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTg            >  2:8347846/1‑93 (MQ=255)
                                                                           aggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTg            <  2:3049932/93‑1 (MQ=255)
                                                                           aggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTg            >  1:2061603/1‑93 (MQ=255)
                                                                           aggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTg            <  1:3135690/93‑1 (MQ=255)
                                                                            ggtGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTgg           <  1:2165796/93‑1 (MQ=255)
                                                                              tGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGAt         >  2:2326181/1‑93 (MQ=255)
                                                                              ggACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGAt         <  1:6209826/92‑1 (MQ=255)
                                                                                gcGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCa       <  2:7323870/92‑1 (MQ=255)
                                                                                aCGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCa       >  1:7992044/1‑93 (MQ=255)
                                                                                aCGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCa       <  1:1681112/93‑1 (MQ=255)
                                                                                aCGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCa       >  1:92618/1‑93 (MQ=255)
                                                                                  gAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACg     >  1:2673784/1‑93 (MQ=255)
                                                                                  gAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACg     >  1:704477/1‑93 (MQ=255)
                                                                                   aaGTTGGCGTTGCTCTGGACGCAGTATAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGc    <  1:5696103/93‑1 (MQ=255)
                                                                                   aaGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATccagc    >  2:7756920/1‑89 (MQ=255)
                                                                                   aaGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGc    <  2:6486372/93‑1 (MQ=255)
                                                                                   aaGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGc    >  2:4074662/1‑93 (MQ=255)
                                                                                   aaGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGc    >  2:5368112/1‑93 (MQ=255)
                                                                                     gTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTg  >  1:7854386/1‑93 (MQ=255)
                                                                                         |                                                                                        
CGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGACGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTG  >  NC_000913/961334‑961511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGACGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAA  >  NC_000913/961330‑961514
                                                                                             |                                                                                           
TTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGG                                                                                             <  SRR4302541.7047325/94‑1 (MQ=60)
  GACGCTAGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCG                                                                                           <  SRR4302541.7639632/94‑1 (MQ=60)
    CGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTT                                                                                         >  SRR4302541.7298792/1‑94 (MQ=60)
    CGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTT                                                                                         <  SRR4302541.2568277/94‑1 (MQ=60)
     GCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTG                                                                                        >  SRR4302541.6935511/1‑94 (MQ=60)
     GCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTG                                                                                        <  SRR4302541.6015601/94‑1 (MQ=60)
     GCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTG                                                                                        >  SRR4302541.5688430/1‑94 (MQ=60)
     CCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTG                                                                                        <  SRR4302541.6015603/94‑1 (MQ=60)
      CTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGC                                                                                       >  SRR4302541.970841/1‑94 (MQ=60)
      CTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGC                                                                                       >  SRR4302541.6282466/1‑94 (MQ=60)
       TGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCT                                                                                      <  SRR4302541.6543761/94‑1 (MQ=60)
        TGTCTGAACTCTGAGTCCGCCCTCCCGGCTGAGCAGTTCAAAATCGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTC                                                                                     <  SRR4302541.7249898/94‑1 (MQ=60)
        GGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAACACGCCCAGGGCGAGCTGGAAATCCAGTGAGGTGACGAAGTTGGCGTTGCTC                                                                                     >  SRR4302541.5876874/1‑94 (MQ=60)
        GGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTC                                                                                     <  SRR4302541.1134704/94‑1 (MQ=60)
        GGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTC                                                                                     >  SRR4302541.5876873/1‑94 (MQ=60)
         GTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCT                                                                                    <  SRR4302541.8262176/94‑1 (MQ=60)
          TCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTG                                                                                   <  SRR4302541.6582138/94‑1 (MQ=60)
            TGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGA                                                                                 >  SRR4302541.6806101/1‑94 (MQ=60)
            TGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGA                                                                                 >  SRR4302541.4772331/1‑94 (MQ=60)
             GAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGAC                                                                                >  SRR4302541.1564532/1‑94 (MQ=60)
             GAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGAC                                                                                <  SRR4302541.8193573/94‑1 (MQ=60)
             GAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGAC                                                                                <  SRR4302541.4664270/94‑1 (MQ=60)
              AAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCGGGAAGGTGACGAAGTTGGCGTTGCTCTGGACG                                                                               >  SRR4302541.3382745/1‑94 (MQ=60)
              AAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACG                                                                               <  SRR4302541.676547/94‑1 (MQ=60)
              AAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACG                                                                               >  SRR4302541.3124991/1‑94 (MQ=60)
               AATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGC                                                                              >  SRR4302541.6356090/1‑94 (MQ=60)
                ATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCA                                                                             >  SRR4302541.1913041/1‑94 (MQ=60)
                ATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGAGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCA                                                                             >  SRR4302541.3987500/1‑94 (MQ=60)
                 TCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAG                                                                            >  SRR4302541.1698483/1‑94 (MQ=60)
                  CTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCGAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGT                                                                           <  SRR4302541.2541208/94‑1 (MQ=60)
                  CTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGT                                                                           <  SRR4302541.8540124/94‑1 (MQ=60)
                    GAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAG                                                                         <  SRR4302541.4620265/94‑1 (MQ=60)
                     AGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGA                                                                        >  SRR4302541.5890993/1‑94 (MQ=60)
                     AGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGA                                                                        <  SRR4302541.1960217/94‑1 (MQ=60)
                      GTCCGCCATCCCGGCTGAGCAGTTCAAAACCGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTGGCTCTGGACGCAGTAGAA                                                                       <  SRR4302541.8618279/94‑1 (MQ=60)
                      GTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAA                                                                       >  SRR4302541.7645219/1‑94 (MQ=60)
                      GTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAA                                                                       >  SRR4302541.7645218/1‑94 (MQ=60)
                      GTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAA                                                                       <  SRR4302541.8618278/94‑1 (MQ=60)
                      GTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAA                                                                       >  SRR4302541.5657598/1‑94 (MQ=60)
                        CCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGA                                                                     <  SRR4302541.2374314/94‑1 (MQ=60)
                         CGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATACAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGAC                                                                    >  SRR4302541.5950676/1‑94 (MQ=60)
                          GCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACG                                                                   <  SRR4302541.5168140/94‑1 (MQ=60)
                          GCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACG                                                                   >  SRR4302541.6105482/1‑94 (MQ=60)
                           CCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGG                                                                  <  SRR4302541.7070946/94‑1 (MQ=60)
                           CCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGG                                                                  <  SRR4302541.6258455/94‑1 (MQ=60)
                            CATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGC                                                                 >  SRR4302541.1051420/1‑94 (MQ=60)
                            CATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGC                                                                 >  SRR4302541.6994189/1‑94 (MQ=60)
                            CATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGC                                                                 <  SRR4302541.6363342/94‑1 (MQ=60)
                             ATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGTAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGAT                                                                >  SRR4302541.4079484/1‑94 (MQ=60)
                             ATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCT                                                                <  SRR4302541.4922026/94‑1 (MQ=60)
                             ATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCT                                                                >  SRR4302541.3098056/1‑94 (MQ=60)
                              TCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTT                                                               <  SRR4302541.2544989/94‑1 (MQ=60)
                              CCCCGCCTGAGCAGATCAAAAACGCCCAGGTCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTT                                                               <  SRR4302541.6958017/94‑1 (MQ=60)
                               CCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTC                                                              <  SRR4302541.7852234/94‑1 (MQ=60)
                               CCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTC                                                              <  SRR4302541.5462079/94‑1 (MQ=60)
                               CCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTC                                                              <  SRR4302541.3908186/94‑1 (MQ=60)
                                CCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCG                                                             <  SRR4302541.3691656/94‑1 (MQ=60)
                                CCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCG                                                             <  SRR4302541.8371859/94‑1 (MQ=60)
                                 CGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGGAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGG                                                            >  SRR4302541.8395514/1‑94 (MQ=60)
                                  GGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGT                                                           >  SRR4302541.7541033/1‑94 (MQ=60)
                                   GCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTG                                                          >  SRR4302541.5304715/1‑94 (MQ=60)
                                   GCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTG                                                          <  SRR4302541.2246559/94‑1 (MQ=60)
                                   GCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTG                                                          >  SRR4302541.7490274/1‑94 (MQ=60)
                                    CTGAGCAGTTCAAAAACGCCCAGGGTGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGA                                                         >  SRR4302541.5953343/1‑94 (MQ=60)
                                      GAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAA                                                       >  SRR4302541.4197680/1‑94 (MQ=60)
                                          AGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCT                                                   <  SRR4302541.279379/94‑1 (MQ=60)
                                          AGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCT                                                   >  SRR4302541.1829245/1‑94 (MQ=60)
                                           GTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTG                                                  >  SRR4302541.5905913/1‑94 (MQ=60)
                                             TCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCT                                                <  SRR4302541.6870902/94‑1 (MQ=60)
                                             TCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCT                                                <  SRR4302541.6104148/94‑1 (MQ=60)
                                              CAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTG                                               >  SRR4302541.7227089/1‑94 (MQ=60)
                                              CAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTG                                               <  SRR4302541.2932290/94‑1 (MQ=60)
                                               AAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGT                                              <  SRR4302541.2761967/94‑1 (MQ=60)
                                               AAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGC                                              >  SRR4302541.1160722/1‑94 (MQ=60)
                                                     GCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTG                                        >  SRR4302541.7080087/1‑94 (MQ=60)
                                                      CCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGA                                       >  SRR4302541.6818331/1‑94 (MQ=60)
                                                       CCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAG                                      >  SRR4302541.7817441/1‑94 (MQ=60)
                                                           GGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAG                                  <  SRR4302541.2870927/94‑1 (MQ=60)
                                                            GCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGC                                 >  SRR4302541.7073967/1‑94 (MQ=60)
                                                             CGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCTCGTGAGAAAGCT                                >  SRR4302541.4707794/1‑94 (MQ=60)
                                                             CGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCT                                <  SRR4302541.5030752/94‑1 (MQ=60)
                                                             CGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCT                                >  SRR4302541.7560139/1‑94 (MQ=60)
                                                             CGAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCT                                <  SRR4302541.1420801/94‑1 (MQ=60)
                                                              GAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTA                               >  SRR4302541.3504175/1‑94 (MQ=60)
                                                              GAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTA                               <  SRR4302541.4705974/94‑1 (MQ=60)
                                                              GAGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTA                               >  SRR4302541.6658762/1‑94 (MQ=60)
                                                              GAGCTGGAAATCCAGGTAGGCGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTA                               >  SRR4302541.2589232/1‑94 (MQ=60)
                                                               AGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAA                              <  SRR4302541.5880558/94‑1 (MQ=60)
                                                               AGCTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAA                              <  SRR4302541.6459602/94‑1 (MQ=60)
                                                                 CTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAAC                            <  SRR4302541.7422686/94‑1 (MQ=60)
                                                                 CTGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAAC                            >  SRR4302541.2662577/1‑94 (MQ=60)
                                                                  TGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACG                           <  SRR4302541.8409981/94‑1 (MQ=60)
                                                                  TGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACG                           <  SRR4302541.4840277/94‑1 (MQ=60)
                                                                  TGGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACG                           >  SRR4302541.738806/1‑94 (MQ=60)
                                                                   GGAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGC                          >  SRR4302541.7844863/1‑94 (MQ=60)
                                                                    GAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTC                         <  SRR4302541.1757490/94‑1 (MQ=60)
                                                                    GAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTC                         <  SRR4302541.3908148/94‑1 (MQ=60)
                                                                    GAAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTC                         <  SRR4302541.5721269/94‑1 (MQ=60)
                                                                     AAATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCA                        <  SRR4302541.354371/94‑1 (MQ=60)
                                                                      AATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCAC                       <  SRR4302541.7061440/94‑1 (MQ=60)
                                                                      AATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCAC                       >  SRR4302541.4377789/1‑94 (MQ=60)
                                                                      AATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCAC                       <  SRR4302541.7538099/94‑1 (MQ=60)
                                                                      AATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTAGGTGAAACTCTGCTGTCCCGGGAGAAAGCTAAAggtgag                       >  SRR4302541.4377791/1‑88 (MQ=60)
                                                                       ATCCAGGTAGGTGACGAAGTTTGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACG                      <  SRR4302541.8647196/94‑1 (MQ=60)
                                                                       ATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACG                      <  SRR4302541.8647195/94‑1 (MQ=60)
                                                                       ATCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACG                      <  SRR4302541.6086258/94‑1 (MQ=60)
                                                                       ATCCAGGTAGGTGACGAAGTTGGCGCTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACG                      >  SRR4302541.3757663/1‑94 (MQ=60)
                                                                        TCCAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGA                     <  SRR4302541.210802/94‑1 (MQ=60)
                                                                          CAGGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAG                   >  SRR4302541.6515907/1‑94 (MQ=60)
                                                                            GGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCC                 <  SRR4302541.565252/94‑1 (MQ=60)
                                                                            GGTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCC                 <  SRR4302541.2374025/94‑1 (MQ=60)
                                                                             GTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCT                <  SRR4302541.3093558/94‑1 (MQ=60)
                                                                             GTAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCT                >  SRR4302541.6251818/1‑94 (MQ=60)
                                                                              TAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTG               >  SRR4302541.2081512/1‑94 (MQ=60)
                                                                              TAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTG               <  SRR4302541.1577243/94‑1 (MQ=60)
                                                                              TAGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTG               <  SRR4302541.528444/94‑1 (MQ=60)
                                                                               AGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGG              >  SRR4302541.8234531/1‑94 (MQ=60)
                                                                               AGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGG              <  SRR4302541.3167379/94‑1 (MQ=60)
                                                                               AGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGG              >  SRR4302541.6975573/1‑94 (MQ=60)
                                                                               AGGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGG              >  SRR4302541.1640110/1‑94 (MQ=60)
                                                                                GGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGA             <  SRR4302541.2186738/94‑1 (MQ=60)
                                                                                GGTGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGA             >  SRR4302541.352082/1‑94 (MQ=60)
                                                                                  GGACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATC           <  SRR4302541.6273288/94‑1 (MQ=60)
                                                                                   GACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCA          >  SRR4302541.3295336/1‑94 (MQ=60)
                                                                                   GACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCA          >  SRR4302541.8080030/1‑94 (MQ=60)
                                                                                   GACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCA          >  SRR4302541.2290819/1‑94 (MQ=60)
                                                                                   GACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCA          <  SRR4302541.8028244/94‑1 (MQ=60)
                                                                                   GACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCA          >  SRR4302541.93425/1‑94 (MQ=60)
                                                                                    ACGAAGTTGGTGTTGCTCTGGACGCAGTAGAAGGCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCAC         <  SRR4302541.7514920/94‑1 (MQ=60)
                                                                                    ACGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCAC         <  SRR4302541.1697643/94‑1 (MQ=60)
                                                                                     CGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACG        <  SRR4302541.8050069/94‑1 (MQ=60)
                                                                                     CGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACG        >  SRR4302541.4504446/1‑94 (MQ=60)
                                                                                     CGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACG        >  SRR4302541.2700347/1‑94 (MQ=60)
                                                                                     CGAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACG        >  SRR4302541.711102/1‑94 (MQ=60)
                                                                                      GAAGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGC       >  SRR4302541.2555050/1‑94 (MQ=60)
                                                                                       AAGTTGGCGTTGCTCTGGACGCAGTATAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCT      <  SRR4302541.5754191/94‑1 (MQ=60)
                                                                                        AGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTG     <  SRR4302541.6084454/94‑1 (MQ=60)
                                                                                        AGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTG     >  SRR4302541.8303719/1‑94 (MQ=60)
                                                                                        AGTTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTG     >  SRR4302541.7940611/1‑94 (MQ=60)
                                                                                          TTGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGA   >  SRR4302541.152024/1‑94 (MQ=60)
                                                                                           TGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAA  >  SRR4302541.3403825/1‑94 (MQ=60)
                                                                                           TGGCGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAA  <  SRR4302541.4304916/94‑1 (MQ=60)
                                                                                             |                                                                                           
TTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGACGTTGCTCTGGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAA  >  NC_000913/961330‑961514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑