Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	946,689	A→G	T144A (ACG→GCG)	b0899 →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	946,689	0	A	G	99.4%	584.5 / NA	172	T144A (ACG→GCG)	b0899	putative transport
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (97/74); total (97/75)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTG  >  NC_000913/946599‑946778

ctctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACAcgcg                                                                                         >  1:4804941/1‑93 (MQ=255)

 tctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGa                                                                                        <  2:4706522/93‑1 (MQ=255)

  ctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTCCCTGGATTAAACACGCGAt                                                                                       <  1:3546859/93‑1 (MQ=255)

  ctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGAt                                                                                       <  2:4915648/93‑1 (MQ=255)

   tATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATg                                                                                      >  1:2727828/1‑93 (MQ=255)

    ttttCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGCGTCAGTACCTGGATTAAACTCGCGATgg                                                                                     <  2:795938/92‑1 (MQ=255)

    aTTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGCAGGGCGGGGTCAGTACCTGGATTAAACACGCGATgg                                                                                     >  1:876122/1‑93 (MQ=255)

    aTTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATgg                                                                                     >  1:5355155/1‑93 (MQ=255)

      ttCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGa                                                                                   <  2:2537926/93‑1 (MQ=255)

       tCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAc                                                                                  >  1:1196487/1‑93 (MQ=255)

        cacaCCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAcc                                                                                 >  1:2494851/1‑93 (MQ=255)

         acacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                <  1:2811231/93‑1 (MQ=255)

         acacCTTATGCGCTAATTGGTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                >  1:3131714/1‑93 (MQ=255)

            ccTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGa                                                                             <  2:4137868/93‑1 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            <  2:5167006/93‑1 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            <  1:1261791/93‑1 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            >  1:4858467/1‑93 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            >  1:3868713/1‑93 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAAGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCAGAc                                                                            >  1:3139385/1‑93 (MQ=255)

              ttATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACt                                                                           <  1:1855453/93‑1 (MQ=255)

               tATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTg                                                                          >  1:6065716/1‑93 (MQ=255)

                    gcTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTa                                                                     <  1:1288356/93‑1 (MQ=255)

                    gcTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAAAACCCGATGGGACCCGGACTGCCTTa                                                                     >  1:4312337/1‑93 (MQ=255)

                       aaTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtct                                                                  <  2:1422753/93‑1 (MQ=255)

                        aTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtctc                                                                 >  2:1443271/1‑93 (MQ=255)

                        aTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtctc                                                                 >  2:3043695/1‑93 (MQ=255)

                         ttgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcg                                                                >  2:511512/1‑93 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               >  1:4035525/1‑93 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               <  2:5276843/93‑1 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               <  1:557179/93‑1 (MQ=255)

                            ttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgctg                                                             <  2:6245704/93‑3 (MQ=255)

                            ttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgccg                                                             <  2:418475/93‑1 (MQ=255)

                             tgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgccgc                                                            <  1:3923687/93‑1 (MQ=255)

                               gTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGt                                                          <  1:4579873/93‑1 (MQ=255)

                                 cAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTgg                                                        >  2:2232075/1‑93 (MQ=255)

                                 cAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTgg                                                        <  1:5575122/93‑1 (MQ=255)

                                  aGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGa                                                       >  1:4602112/1‑93 (MQ=255)

                                  aGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGa                                                       >  1:5905220/1‑93 (MQ=255)

                                   gTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAc                                                      >  2:2982365/1‑93 (MQ=255)

                                   gTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAc                                                      >  2:1320120/1‑93 (MQ=255)

                                   gTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAc                                                      >  1:4219367/1‑93 (MQ=255)

                                    ttAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAcc                                                     >  2:6037456/1‑93 (MQ=255)

                                     tAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCt                                                    <  1:603004/93‑1 (MQ=255)

                                     tAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCt                                                    <  1:300717/93‑1 (MQ=255)

                                      aGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCtc                                                   >  2:5480444/1‑92 (MQ=255)

                                      aGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTa                                                   >  2:2162408/1‑93 (MQ=255)

                                       ggCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTAc                                                  >  2:6306929/1‑93 (MQ=255)

                                        gCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACt                                                 >  2:229405/1‑93 (MQ=255)

                                         cTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTg                                                <  2:3614179/93‑1 (MQ=255)

                                         cTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTg                                                <  2:176961/93‑1 (MQ=255)

                                          tCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTgg                                               >  1:3648280/1‑93 (MQ=255)

                                          tCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTgg                                               >  2:4415076/1‑93 (MQ=255)

                                           cGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTggg                                              >  1:3759667/1‑93 (MQ=255)

                                            gACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggt                                             >  2:5207613/1‑93 (MQ=255)

                                            gACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggt                                             <  2:569245/93‑1 (MQ=255)

                                             aCCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggtg                                            <  1:970882/93‑1 (MQ=255)

                                                ttCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGgtggtg                                         <  2:2661572/93‑1 (MQ=255)

                                                 tCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGc                                        >  2:5207926/1‑93 (MQ=255)

                                                  cAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCa                                       <  2:1214018/93‑1 (MQ=255)

                                                   aaaGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCat                                      <  2:2177742/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  1:1045274/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  2:432508/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  2:335142/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  1:3781737/93‑1 (MQ=255)

                                                       aTGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTc                                  <  2:5616108/93‑1 (MQ=255)

                                                        tGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTcc                                 <  2:3082908/93‑1 (MQ=255)

                                                         tggAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTccc                                <  2:3160584/92‑1 (MQ=255)

                                                         gggAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTccc                                <  1:5820011/93‑1 (MQ=255)

                                                          ggAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCt                               >  2:3480060/1‑93 (MQ=255)

                                                           gAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGGGCCTATTCCCTa                              >  2:1671183/1‑93 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  1:656751/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  2:6134298/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            >  2:885351/1‑93 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  1:4712455/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            >  2:2990175/1‑93 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGGGCATATTCCCTATc                            >  1:5126669/1‑93 (MQ=255)

                                                                gCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTgg                         <  1:1471383/93‑1 (MQ=255)

                                                                  ggggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGca                       >  2:3755321/1‑93 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  1:3281031/1‑93 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  1:6263091/1‑93 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  1:5214917/1‑93 (MQ=255)

                                                                      tCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACaaa                   >  2:4817528/1‑93 (MQ=255)

                                                                        aGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACaaaaa                 >  2:1594745/1‑93 (MQ=255)

                                                                         gTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAc                >  1:1632510/1‑93 (MQ=255)

                                                                         gTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGCGGTGCATATTCGCTATCTGCTACAAAAAc                >  1:967008/1‑93 (MQ=255)

                                                                         ctACCTGGATTAAACACGCGATGGGCCCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAc                <  1:191050/92‑1 (MQ=255)

                                                                            ccTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAcccc             <  1:5939853/93‑1 (MQ=255)

                                                                             cTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCa            <  1:2656654/93‑1 (MQ=255)

                                                                              tGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAg           <  2:1737130/93‑1 (MQ=255)

                                                                               ggATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAgg          <  2:728197/93‑1 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  2:580064/1‑93 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  2:77886/1‑93 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  1:2087635/1‑93 (MQ=255)

                                                                                   tAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAt      <  2:4550548/93‑1 (MQ=255)

                                                                                   tAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAt      >  1:3024185/1‑93 (MQ=255)

                                                                                     aaCACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTc    >  1:444502/1‑93 (MQ=255)

                                                                                     aaCACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTc    >  2:6451498/1‑93 (MQ=255)

                                                                                      acacgcGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCt   >  2:4662145/1‑93 (MQ=255)

                                                                                      acacgcGATGGGACCCGGACTGGCTTATCTCGCCGCGGGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCTCAGGCAAGTCt   >  2:3991320/1‑93 (MQ=255)

                                                                                       cacgcGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTg  >  1:6116271/1‑93 (MQ=255)

CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTG  >  NC_000913/946599‑946778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGATTG  >  NC_000913/946596‑946782

GCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACG                                                                                               <  SRR4302534.2310896/94‑1 (MQ=60)

GCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACG                                                                                               >  SRR4302534.1187317/1‑94 (MQ=60)

GCACTCTATTTCACACCTTACGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACG                                                                                               >  SRR4302534.6479897/1‑94 (MQ=60)

 CACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGC                                                                                              >  SRR4302534.2697104/1‑94 (MQ=60)

  CCTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCG                                                                                             <  SRR4302534.1702467/94‑1 (MQ=60)

  ACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCG                                                                                             >  SRR4302534.4935398/1‑94 (MQ=60)

  ACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCG                                                                                             >  SRR4302534.1246330/1‑94 (MQ=60)

     CTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTCCCTGGATTAAACACGCGATG                                                                                          <  SRR4302534.3642252/94‑1 (MQ=60)

     CTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATG                                                                                          >  SRR4302534.2799771/1‑94 (MQ=60)

      TATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGCAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGG                                                                                         >  SRR4302534.898284/1‑94 (MQ=60)

      TATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGG                                                                                         >  SRR4302534.5499522/1‑94 (MQ=60)

      TATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGG                                                                                         >  SRR4302534.22224/1‑94 (MQ=60)

         TTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAC                                                                                      >  SRR4302534.1227969/1‑94 (MQ=60)

          TCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACC                                                                                     >  SRR4302534.2561112/1‑94 (MQ=60)

           CACACCTTATGCGCTAATTGGTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCC                                                                                    >  SRR4302534.3214038/1‑94 (MQ=60)

            ACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCG                                                                                   >  SRR4302534.140916/1‑94 (MQ=60)

            ACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCG                                                                                   >  SRR4302534.1667931/1‑94 (MQ=60)

            ACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCG                                                                                   >  SRR4302534.4902217/1‑94 (MQ=60)

            ACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCG                                                                                   <  SRR4302534.2885141/94‑1 (MQ=60)

              ACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGA                                                                                 >  SRR4302534.4348780/1‑94 (MQ=60)

               CCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAC                                                                                >  SRR4302534.3972588/1‑94 (MQ=60)

               CCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAC                                                                                >  SRR4302534.4990283/1‑94 (MQ=60)

               CCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAAGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCAGAC                                                                                >  SRR4302534.3221949/1‑94 (MQ=60)

                CTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACT                                                                               <  SRR4302534.2620695/94‑1 (MQ=60)

                CTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACT                                                                               >  SRR4302534.5232757/1‑94 (MQ=60)

                CTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACT                                                                               <  SRR4302534.1295036/94‑1 (MQ=60)

                 TTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTG                                                                              >  SRR4302534.6230636/1‑94 (MQ=60)

                 TTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTG                                                                              >  SRR4302534.5428904/1‑94 (MQ=60)

                 TTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTG                                                                              <  SRR4302534.1903736/94‑1 (MQ=60)

                      CGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTA                                                                         >  SRR4302534.5676649/1‑94 (MQ=60)

                      CGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAAAACCCGATGGGACCCGGACTGCCTTA                                                                         >  SRR4302534.4429159/1‑94 (MQ=60)

                       GCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAT                                                                        <  SRR4302534.1322284/94‑1 (MQ=60)

                            TTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGC                                                                   >  SRR4302534.4143713/1‑94 (MQ=60)

                             TGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCC                                                                  <  SRR4302534.570914/94‑1 (MQ=60)

                                TGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCG                                                               >  SRR4302534.3296514/1‑94 (MQ=60)

                                TGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCG                                                               <  SRR4302534.4028808/94‑1 (MQ=60)

                                 GGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGT                                                              >  SRR4302534.1922240/1‑94 (MQ=60)

                                 GGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATATCGCCGCGT                                                              <  SRR4302534.6473595/94‑1 (MQ=60)

                                  GTCAGTTATGCTCGACCTTCAAAGATGGGAAGGTCGCGGTCAGTACCTGGATTTAACACGCGATTGGACCCGGACTGTCTTATCTCGCCTCGTG                                                             <  SRR4302534.2226157/94‑1 (MQ=60)

                                  GTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTG                                                             <  SRR4302534.4704091/94‑1 (MQ=60)

                                   TCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGG                                                            <  SRR4302534.4289925/94‑1 (MQ=60)

                                    CAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGA                                                           >  SRR4302534.4727008/1‑94 (MQ=60)

                                    CAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGA                                                           <  SRR4302534.5725702/94‑1 (MQ=60)

                                    CAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGA                                                           <  SRR4302534.4084503/94‑1 (MQ=60)

                                    CAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGA                                                           >  SRR4302534.6065569/1‑94 (MQ=60)

                                    CAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGA                                                           >  SRR4302534.2984609/1‑94 (MQ=60)

                                     AGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAC                                                          >  SRR4302534.4333123/1‑94 (MQ=60)

                                        TAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTA                                                       <  SRR4302534.308484/94‑1 (MQ=60)

                                        TAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTA                                                       <  SRR4302534.617697/94‑1 (MQ=60)

                                         AGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTAC                                                      >  SRR4302534.1311264/1‑94 (MQ=60)

                                         AGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTAC                                                      >  SRR4302534.955674/1‑94 (MQ=60)

                                          GGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACT                                                     <  SRR4302534.6088071/94‑1 (MQ=60)

                                            CTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGG                                                   >  SRR4302534.3746320/1‑94 (MQ=60)

                                             TCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGG                                                  >  SRR4302534.3860624/1‑94 (MQ=60)

                                             TCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGG                                                  >  SRR4302534.760788/1‑94 (MQ=60)

                                              CGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGT                                                 >  SRR4302534.6200555/1‑94 (MQ=60)

                                               GACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTG                                                >  SRR4302534.5761197/1‑94 (MQ=60)

                                                ACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGG                                               <  SRR4302534.995616/94‑1 (MQ=60)

                                                       AAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATAT                                        >  SRR4302534.3199842/1‑94 (MQ=60)

                                                        AGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATT                                       >  SRR4302534.4598685/1‑94 (MQ=60)

                                                        AGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATT                                       <  SRR4302534.1072285/94‑1 (MQ=60)

                                                        AGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATT                                       <  SRR4302534.3883273/94‑1 (MQ=60)

                                                           TGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCC                                    <  SRR4302534.5330733/94‑1 (MQ=60)

                                                            GGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCT                                   <  SRR4302534.5977984/94‑1 (MQ=60)

                                                             GGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTA                                  <  SRR4302534.3530697/94‑1 (MQ=60)

                                                             GGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTA                                  <  SRR4302534.287579/94‑1 (MQ=60)

                                                               AAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATC                                >  SRR4302534.3871273/1‑94 (MQ=60)

                                                               AAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGGGCATATTCCCTATC                                >  SRR4302534.5265083/1‑94 (MQ=60)

                                                                AGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCT                               <  SRR4302534.4840433/94‑1 (MQ=60)

                                                                AGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCT                               <  SRR4302534.672743/94‑1 (MQ=60)

                                                                  GGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGG                             >  SRR4302534.3997327/1‑94 (MQ=60)

                                                                   GCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGC                            <  SRR4302534.1510081/94‑1 (MQ=60)

                                                                     GGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCAC                          >  SRR4302534.6432734/1‑94 (MQ=60)

                                                                     GGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCAC                          >  SRR4302534.3368476/1‑94 (MQ=60)

                                                                     GGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCAC                          >  SRR4302534.6432732/1‑94 (MQ=60)

                                                                     GGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCAC                          >  SRR4302534.5355663/1‑94 (MQ=60)

                                                                           AGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAC                    >  SRR4302534.1675070/1‑94 (MQ=60)

                                                                           AGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGCGGTGCATATTCGCTATCTGCTACAAAAAC                    >  SRR4302534.991647/1‑94 (MQ=60)

                                                                            GTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACC                   >  SRR4302534.4764069/1‑94 (MQ=60)

                                                                            CTACCTGGATTAAACACGCGATGGGCCCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACC                   <  SRR4302534.196057/94‑1 (MQ=60)

                                                                              ACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCC                 >  SRR4302534.6002466/1‑94 (MQ=60)

                                                                              ACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCC                 <  SRR4302534.6119242/94‑1 (MQ=60)

                                                                              ACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGCACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCC                 <  SRR4302534.2057224/94‑1 (MQ=60)

                                                                               CCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCA                <  SRR4302534.6101135/94‑1 (MQ=60)

                                                                                CTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAG               <  SRR4302534.2726890/94‑1 (MQ=60)

                                                                                    ATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAA           >  SRR4302534.2142349/1‑94 (MQ=60)

                                                                                     TTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAT          >  SRR4302534.3103439/1‑94 (MQ=60)

                                                                                       AAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTC        <  SRR4302534.471296/94‑1 (MQ=60)

                                                                                       AAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTC        >  SRR4302534.3099367/1‑94 (MQ=60)

                                                                                       AAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTC        >  SRR4302534.3697338/1‑94 (MQ=60)

                                                                                       AAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTC        >  SRR4302534.455786/1‑94 (MQ=60)

                                                                                        AACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCT       <  SRR4302534.3795314/94‑1 (MQ=60)

                                                                                         ACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTG      >  SRR4302534.6282653/1‑94 (MQ=60)

                                                                                          CACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGA     >  SRR4302534.532604/1‑94 (MQ=60)

                                                                                           ACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGAT    >  SRR4302534.3634892/1‑94 (MQ=60)

                                                                                           ACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGAT    <  SRR4302534.78696/94‑1 (MQ=60)

                                                                                           ACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGAT    <  SRR4302534.1007760/94‑1 (MQ=60)

                                                                                            CGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGATT   <  SRR4302534.6353394/94‑1 (MQ=60)

                                                                                             GCGATGGGTCCCGGACTGGCTTATCTCGCCGCGTGGACCATCTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGATTG  <  SRR4302534.4001760/94‑1 (MQ=60)

                                                                                             GCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGATTG  <  SRR4302534.5098778/94‑1 (MQ=60)

GCACTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTGATTG  >  NC_000913/946596‑946782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`