Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,875	C→T	P2S (CCG→TCG)	mukE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,875	0	C	T	99.6%	1073.4 / ‑5.1	275	P2S (CCG→TCG)	mukE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (148/126); minor base G (1/0); total (149/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 278 total aligned reads displayed.

TCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAA  >  NC_000913/974785‑974964

  aCCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGGGCATGTCATCGACAAATATTGAACAAGTGATGTCGGt                                                                                       >  1:1621792/1‑93 (MQ=255)

  aCCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGt                                                                                       >  2:2731122/1‑93 (MQ=255)

  aCCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGt                                                                                       <  2:2216201/93‑1 (MQ=255)

    cGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTa                                                                                     <  1:3307155/93‑1 (MQ=255)

     ggACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTaa                                                                                    <  2:328219/93‑1 (MQ=255)

      gACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAg                                                                                   >  2:2944572/1‑93 (MQ=255)

      gACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAg                                                                                   >  2:589670/1‑93 (MQ=255)

      gACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAg                                                                                   >  1:2391924/1‑93 (MQ=255)

      gACTGCCAGCGAAATGGCAGCCGATTAATGATTAAGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAGGTTGTGGGGGTTAAg                                                                                   >  1:4956840/1‑93 (MQ=255)

         tGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTg                                                                                >  2:4783305/1‑93 (MQ=255)

          gCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTgg                                                                               >  1:3645088/1‑93 (MQ=255)

             aGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGgcgc                                                                            <  1:5789553/93‑1 (MQ=255)

                 aaaTGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGgc                                                                        >  2:737766/1‑93 (MQ=255)

                  aaTGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGgcg                                                                       >  1:647731/1‑93 (MQ=255)

                   aTGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGgcgc                                                                      <  2:3584257/93‑1 (MQ=255)

                   aTGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGgcgc                                                                      >  1:1332850/1‑93 (MQ=255)

                    tGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCt                                                                     >  1:4736842/1‑93 (MQ=255)

                     ggCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTg                                                                    <  2:499801/93‑1 (MQ=255)

                     ggCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTg                                                                    >  1:774998/1‑93 (MQ=255)

                     ggCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTg                                                                    >  2:1577279/1‑93 (MQ=255)

                      gCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTgg                                                                   >  2:228980/1‑93 (MQ=255)

                       cAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGc                                                                  <  2:4943012/93‑1 (MQ=255)

                        aGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCg                                                                 <  2:5849650/93‑1 (MQ=255)

                        aGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCg                                                                 >  1:4479801/1‑93 (MQ=255)

                            gATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATc                                                             <  1:6285341/93‑1 (MQ=255)

                             aTTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATcc                                                            >  1:2694874/1‑93 (MQ=255)

                              ttAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCg                                                           >  1:1672462/1‑93 (MQ=255)

                               tAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGt                                                          <  1:5684540/93‑1 (MQ=255)

                               tAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGt                                                          >  1:3832269/1‑93 (MQ=255)

                               tAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGt                                                          >  2:4686524/1‑93 (MQ=255)

                               tAATGATTACGGAGCCAAGGCACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTCAGCTGGCGCAGGCGCTAGCGAATCCGt                                                          >  1:1539026/1‑93 (MQ=255)

                                aaTGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGCATCCGtt                                                         >  2:962411/1‑93 (MQ=255)

                                aaTGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGtt                                                         <  1:2910498/93‑1 (MQ=255)

                                  tGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTAt                                                       <  2:1737268/93‑1 (MQ=255)

                                  tGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTAt                                                       <  2:5299279/93‑1 (MQ=255)

                                     ttACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTc                                                    <  2:3078750/93‑1 (MQ=255)

                                     ttACGGAGCCAAGATACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTc                                                    <  1:1847213/93‑1 (MQ=255)

                                       aCGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCg                                                  <  1:5273083/93‑1 (MQ=255)

                                         ggAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTCAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGgc                                                >  2:1337560/1‑93 (MQ=255)

                                          gAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGgcg                                               >  2:6091782/1‑93 (MQ=255)

                                          gAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGgcg                                               >  2:4168464/1‑93 (MQ=255)

                                          gAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGgcg                                               >  1:4865869/1‑93 (MQ=255)

                                           aGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGgcgc                                              <  2:5834365/93‑1 (MQ=255)

                                           aGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGgcgc                                              <  2:2850097/93‑1 (MQ=255)

                                            gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCt                                             >  1:19157/1‑93 (MQ=255)

                                             ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTg                                            <  1:5552740/93‑1 (MQ=255)

                                              cAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTgg                                           >  2:2711613/1‑93 (MQ=255)

                                               aaGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGa                                          <  1:1158926/93‑1 (MQ=255)

                                               aaGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGa                                          <  1:1959336/93‑1 (MQ=255)

                                               aaGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGa                                          >  2:1396915/1‑93 (MQ=255)

                                                aGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAc                                         >  2:5036197/1‑93 (MQ=255)

                                                aGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAc                                         >  1:2507002/1‑93 (MQ=255)

                                                  gTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAg                                       <  1:2217065/93‑1 (MQ=255)

                                                  gTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAg                                       >  1:690205/1‑93 (MQ=255)

                                                   tACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgc                                      >  2:6291761/1‑93 (MQ=255)

                                                   tACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgc                                      <  2:5128631/93‑1 (MQ=255)

                                                     cAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgcgc                                    >  1:520717/1‑93 (MQ=255)

                                                      aGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGcc                                   >  2:17985/1‑93 (MQ=255)

                                                       ggCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCGGGCGCTGGCCAGCGCCt                                  >  1:2775633/1‑93 (MQ=255)

                                                       ggCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCt                                  <  1:1061787/93‑1 (MQ=255)

                                                       ggCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCt                                  >  2:45831/1‑93 (MQ=255)

                                                          gcATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTAc                               >  2:1068423/1‑93 (MQ=255)

                                                          gcATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTAc                               >  2:449432/1‑93 (MQ=255)

                                                          gcATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTAc                               >  2:3819845/1‑93 (MQ=255)

                                                           cATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACg                              >  2:5960013/1‑93 (MQ=255)

                                                            aTGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGt                             >  1:4033936/1‑93 (MQ=255)

                                                            aTGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGt                             >  1:6070657/1‑93 (MQ=255)

                                                             tGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGtt                            <  1:4546776/93‑1 (MQ=255)

                                                              gTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTc                           >  1:3966611/1‑93 (MQ=255)

                                                               tCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCa                          >  1:3573160/1‑93 (MQ=255)

                                                                cATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAg                         >  1:6093538/1‑93 (MQ=255)

                                                                 aTCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAgg                        >  2:6387408/1‑93 (MQ=255)

                                                                 aTCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAgg                        <  2:4245178/93‑1 (MQ=255)

                                                                 aTCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAgg                        <  1:2085251/93‑1 (MQ=255)

                                                                    gACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACg                     >  1:562392/1‑93 (MQ=255)

                                                                    gACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACg                     >  2:5087938/1‑93 (MQ=255)

                                                                    gACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACg                     >  1:4223017/1‑93 (MQ=255)

                                                                       aaaTATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCa                  <  2:847240/93‑1 (MQ=255)

                                                                       aaaTATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCa                  <  2:5406322/93‑1 (MQ=255)

                                                                         atatTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCata                <  2:3127338/93‑1 (MQ=255)

                                                                          tatTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCatat               <  1:3045247/93‑1 (MQ=255)

                                                                          tatTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCatat               >  2:3524532/1‑93 (MQ=255)

                                                                           atTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATAtt              >  2:3905875/1‑93 (MQ=255)

                                                                           atTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATAtt              <  2:6184220/93‑1 (MQ=255)

                                                                             tGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTgg            <  1:2278456/93‑1 (MQ=255)

                                                                             tGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATACGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTgg            <  2:263010/93‑1 (MQ=255)

                                                                              gAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGc           >  2:2001657/1‑93 (MQ=255)

                                                                                aCAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCt         <  1:2025554/93‑1 (MQ=255)

                                                                                aCAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCt         <  1:928382/93‑1 (MQ=255)

                                                                                aCAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCt         >  1:5866182/1‑93 (MQ=255)

                                                                                aCAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCt         >  2:247981/1‑93 (MQ=255)

                                                                                 cAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTc        <  2:5771875/93‑1 (MQ=255)

                                                                                 cAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTc        <  2:897971/93‑1 (MQ=255)

                                                                                    gTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgac     >  2:5217266/1‑93 (MQ=255)

                                                                                    gTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgac     >  1:5509801/1‑93 (MQ=255)

                                                                                    gTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgac     <  2:5991304/93‑1 (MQ=255)

                                                                                     tGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgacg    <  1:4974344/93‑1 (MQ=255)

                                                                                      gATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgacga   <  1:5392474/93‑1 (MQ=255)

                                                                                      gATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgacga   <  1:1764513/93‑1 (MQ=255)

                                                                                       aTGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGaa  <  2:2571598/93‑1 (MQ=255)

TCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAA  >  NC_000913/974785‑974964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGG  >  NC_000913/974782‑974968

ATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGT                                                                                               >  SRR4302534.5947738/1‑94 (MQ=60)

 TTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTC                                                                                              >  SRR4302534.5450338/1‑94 (MQ=60)

 TTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTC                                                                                              >  SRR4302534.4287643/1‑94 (MQ=60)

   TCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGTGCATGTCATCGACAAATATTGAACAAGTGATGTCGG                                                                                            <  SRR4302534.5774083/94‑1 (MQ=60)

    CACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGGGCATGTCATCGACAAATATTGAACAAGTGATGTCGGT                                                                                           >  SRR4302534.1664099/1‑94 (MQ=60)

    CACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGT                                                                                           >  SRR4302534.5004395/1‑94 (MQ=60)

     ACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTT                                                                                          >  SRR4302534.2913301/1‑94 (MQ=60)

      CCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTA                                                                                         <  SRR4302534.1895426/94‑1 (MQ=60)

       CGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAA                                                                                        <  SRR4302534.3395465/94‑1 (MQ=60)

       CGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAA                                                                                        <  SRR4302534.6407627/94‑1 (MQ=60)

       CGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAA                                                                                        <  SRR4302534.2108553/94‑1 (MQ=60)

       CGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAA                                                                                        <  SRR4302534.4823080/94‑1 (MQ=60)

        GGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAG                                                                                       >  SRR4302534.231374/1‑94 (MQ=60)

        GGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAG                                                                                       >  SRR4302534.2455490/1‑94 (MQ=60)

        GGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAG                                                                                       >  SRR4302534.2061153/1‑94 (MQ=60)

        GGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAG                                                                                       >  SRR4302534.4135282/1‑94 (MQ=60)

           CTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTG                                                                                    >  SRR4302534.4041201/1‑94 (MQ=60)

            TGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGG                                                                                   >  SRR4302534.2509898/1‑94 (MQ=60)

            TGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGG                                                                                   >  SRR4302534.3743036/1‑94 (MQ=60)

             GCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGC                                                                                  <  SRR4302534.741279/94‑1 (MQ=60)

              CCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCG                                                                                 <  SRR4302534.3567596/94‑1 (MQ=60)

                AGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCA                                                                               <  SRR4302534.5946618/94‑1 (MQ=60)

                  CGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGG                                                                             <  SRR4302534.3736610/94‑1 (MQ=60)

                    AAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCG                                                                           >  SRR4302534.663436/1‑94 (MQ=60)

                     AATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGC                                                                          >  SRR4302534.1367985/1‑94 (MQ=60)

                      ATGGCAGCTGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATGGAACAAGTGATGTCGGTTAAGGTGGCGCAGGCGCT                                                                         <  SRR4302534.4086258/94‑1 (MQ=60)

                      ATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCT                                                                         <  SRR4302534.3916434/94‑1 (MQ=60)

                      ATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCT                                                                         <  SRR4302534.5080370/94‑1 (MQ=60)

                      ATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCT                                                                         >  SRR4302534.5780899/1‑94 (MQ=60)

                      ATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCT                                                                         >  SRR4302534.4865454/1‑94 (MQ=60)

                       TGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTG                                                                        >  SRR4302534.794309/1‑94 (MQ=60)

                        GGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGG                                                                       <  SRR4302534.1879726/94‑1 (MQ=60)

                        GGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGG                                                                       <  SRR4302534.592350/94‑1 (MQ=60)

                         GCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGC                                                                      <  SRR4302534.546917/94‑1 (MQ=60)

                         GCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGC                                                                      <  SRR4302534.582129/94‑1 (MQ=60)

                          CAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCG                                                                     <  SRR4302534.1013697/94‑1 (MQ=60)

                          CAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCG                                                                     >  SRR4302534.4600997/1‑94 (MQ=60)

                             CCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGCAT                                                                  >  SRR4302534.3129412/1‑94 (MQ=60)

                              CGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATC                                                                 >  SRR4302534.6554557/1‑94 (MQ=60)

                              CGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATC                                                                 >  SRR4302534.6154066/1‑94 (MQ=60)

                               GATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCC                                                                <  SRR4302534.6455397/94‑1 (MQ=60)

                               GATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCC                                                                <  SRR4302534.1797825/94‑1 (MQ=60)

                               GATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCC                                                                >  SRR4302534.2766080/1‑94 (MQ=60)

                                ATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCG                                                               >  SRR4302534.2618747/1‑94 (MQ=60)

                                ATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCG                                                               >  SRR4302534.1715983/1‑94 (MQ=60)

                                ATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCG                                                               <  SRR4302534.6139926/94‑1 (MQ=60)

                                 TTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGT                                                              >  SRR4302534.3935189/1‑94 (MQ=60)

                                 TTAATGATTACGGAGCCAAGGCACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTCAGCTGGCGCAGGCGCTAGCGAATCCGT                                                              >  SRR4302534.1579377/1‑94 (MQ=60)

                                  TAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTT                                                             <  SRR4302534.5838479/94‑1 (MQ=60)

                                  TAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTT                                                             <  SRR4302534.2582268/94‑1 (MQ=60)

                                  TAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTT                                                             <  SRR4302534.2839925/94‑1 (MQ=60)

                                  TAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTT                                                             <  SRR4302534.665625/94‑1 (MQ=60)

                                  TAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTT                                                             >  SRR4302534.1999399/1‑94 (MQ=60)

                                  TAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTT                                                             >  SRR4302534.2500535/1‑94 (MQ=60)

                                   AATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTA                                                            <  SRR4302534.2986900/94‑1 (MQ=60)

                                   AATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTA                                                            >  SRR4302534.4030639/1‑94 (MQ=60)

                                    ATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTAT                                                           <  SRR4302534.5486447/94‑1 (MQ=60)

                                     TGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATT                                                          >  SRR4302534.2867198/1‑94 (MQ=60)

                                      GATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTT                                                         >  SRR4302534.1172810/1‑94 (MQ=60)

                                        TTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCC                                                       >  SRR4302534.6255643/1‑94 (MQ=60)

                                        TTACGGAGCCAAGATACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCC                                                       <  SRR4302534.1895272/94‑1 (MQ=60)

                                         TACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCG                                                      <  SRR4302534.1889613/94‑1 (MQ=60)

                                         TACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCG                                                      <  SRR4302534.4428610/94‑1 (MQ=60)

                                          ACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGG                                                     <  SRR4302534.5415507/94‑1 (MQ=60)

                                            GGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCG                                                   >  SRR4302534.4997859/1‑94 (MQ=60)

                                             GAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGC                                                  >  SRR4302534.5738785/1‑94 (MQ=60)

                                              AGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCT                                                 >  SRR4302534.19663/1‑94 (MQ=60)

                                               GCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTG                                                <  SRR4302534.1796381/94‑1 (MQ=60)

                                               GCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTG                                                >  SRR4302534.514521/1‑94 (MQ=60)

                                               GCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTG                                                <  SRR4302534.6006152/94‑1 (MQ=60)

                                                CCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGG                                               <  SRR4302534.5702642/94‑1 (MQ=60)

                                                 CAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGA                                              >  SRR4302534.3706136/1‑94 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             >  SRR4302534.5603951/1‑94 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             >  SRR4302534.4213749/1‑94 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             <  SRR4302534.1189335/94‑1 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             >  SRR4302534.6615469/1‑94 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             <  SRR4302534.2010611/94‑1 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             <  SRR4302534.3543863/94‑1 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             >  SRR4302534.2573574/1‑94 (MQ=60)

                                                  AAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAC                                             >  SRR4302534.9489/1‑94 (MQ=60)

                                                    GGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAG                                           >  SRR4302534.707090/1‑94 (MQ=60)

                                                    GGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAG                                           <  SRR4302534.2181328/94‑1 (MQ=60)

                                                     GTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGC                                          <  SRR4302534.5095751/94‑1 (MQ=60)

                                                     GTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGC                                          <  SRR4302534.2275775/94‑1 (MQ=60)

                                                     GTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCCGCGCTGGACAGC                                          >  SRR4302534.16295/1‑94 (MQ=60)

                                                       ACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGC                                        >  SRR4302534.533661/1‑94 (MQ=60)

                                                       ACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGC                                        >  SRR4302534.6258643/1‑94 (MQ=60)

                                                       ACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGC                                        >  SRR4302534.2814783/1‑94 (MQ=60)

                                                         AGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCGGGCGCTGGCCAGCGCCT                                      >  SRR4302534.2848656/1‑94 (MQ=60)

                                                         AGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCT                                      >  SRR4302534.1316449/1‑94 (MQ=60)

                                                         AGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCT                                      >  SRR4302534.1483024/1‑94 (MQ=60)

                                                         AGGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCT                                      >  SRR4302534.6371540/1‑94 (MQ=60)

                                                          GGCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTT                                     <  SRR4302534.1089304/94‑1 (MQ=60)

                                                           GCGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTA                                    <  SRR4302534.5370331/94‑1 (MQ=60)

                                                            CGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTAC                                   >  SRR4302534.5675352/1‑94 (MQ=60)

                                                            CGCATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTAC                                   <  SRR4302534.803950/94‑1 (MQ=60)

                                                              CATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGT                                 >  SRR4302534.4142072/1‑94 (MQ=60)

                                                              CATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGT                                 >  SRR4302534.6235706/1‑94 (MQ=60)

                                                               ATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTT                                >  SRR4302534.2099097/1‑94 (MQ=60)

                                                               ATGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTT                                <  SRR4302534.5369771/94‑1 (MQ=60)

                                                                TGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTC                               >  SRR4302534.4072788/1‑94 (MQ=60)

                                                                TGTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTC                               <  SRR4302534.4670038/94‑1 (MQ=60)

                                                                 GTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCA                              >  SRR4302534.3669267/1‑94 (MQ=60)

                                                                 GTCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCA                              <  SRR4302534.1970485/94‑1 (MQ=60)

                                                                  TCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAG                             >  SRR4302534.6259239/1‑94 (MQ=60)

                                                                  TCATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAG                             >  SRR4302534.4343321/1‑94 (MQ=60)

                                                                    ATCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGA                           <  SRR4302534.2139894/94‑1 (MQ=60)

                                                                     TCGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGAC                          <  SRR4302534.1916977/94‑1 (MQ=60)

                                                                      CGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACG                         >  SRR4302534.1623465/1‑94 (MQ=60)

                                                                      CGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACG                         >  SRR4302534.4336853/1‑94 (MQ=60)

                                                                      CGACAAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACG                         >  SRR4302534.576229/1‑94 (MQ=60)

                                                                          AAATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCATGACGCCAT                     >  SRR4302534.1424730/1‑94 (MQ=60)

                                                                           AATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATA                    <  SRR4302534.5128193/94‑1 (MQ=60)

                                                                           AATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATA                    <  SRR4302534.5847698/94‑1 (MQ=60)

                                                                           AATATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATA                    >  SRR4302534.4258095/1‑94 (MQ=60)

                                                                             TATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGCCAGCGCCTTACGTTCCGGACGACATATT                  >  SRR4302534.4752915/1‑94 (MQ=60)

                                                                             TATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATT                  >  SRR4302534.4948695/1‑94 (MQ=60)

                                                                             TATTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATT                  <  SRR4302534.3125120/94‑1 (MQ=60)

                                                                              ATTGAACAAGTGATGGCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCCGGACGCCATATTG                 >  SRR4302534.2252860/1‑94 (MQ=60)

                                                                               TTGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGG                <  SRR4302534.752080/94‑1 (MQ=60)

                                                                                TGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGC               >  SRR4302534.1696459/1‑94 (MQ=60)

                                                                                TGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGC               <  SRR4302534.2275846/94‑1 (MQ=60)

                                                                                TGAACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGC               <  SRR4302534.2338913/94‑1 (MQ=60)

                                                                                  AACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCT             >  SRR4302534.6025467/1‑94 (MQ=60)

                                                                                  AACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCT             >  SRR4302534.3140426/1‑94 (MQ=60)

                                                                                   ACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTC            <  SRR4302534.2452567/94‑1 (MQ=60)

                                                                                   ACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTC            <  SRR4302534.952008/94‑1 (MQ=60)

                                                                                   ACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTC            >  SRR4302534.1189583/1‑94 (MQ=60)

                                                                                   ACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTC            >  SRR4302534.4894041/1‑94 (MQ=60)

                                                                                   ACAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTC            <  SRR4302534.2078542/94‑1 (MQ=60)

                                                                                    CAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGCCAGCGCCTTCCGTTAAGGAGGCCATATTGGCCTCG           >  SRR4302534.5539630/1‑94 (MQ=60)

                                                                                    CAAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCG           <  SRR4302534.3117937/94‑1 (MQ=60)

                                                                                     AAGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGA          >  SRR4302534.4344794/1‑94 (MQ=60)

                                                                                      AGTGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGAC         >  SRR4302534.5658430/1‑94 (MQ=60)

                                                                                        TGATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGA       <  SRR4302534.5109003/94‑1 (MQ=60)

                                                                                         GATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAA      <  SRR4302534.4618317/94‑1 (MQ=60)

                                                                                         GATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAA      <  SRR4302534.1810312/94‑1 (MQ=60)

                                                                                         GATGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAA      <  SRR4302534.5537680/94‑1 (MQ=60)

                                                                                           TGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACT    <  SRR4302534.5336255/94‑1 (MQ=60)

                                                                                           TGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACT    <  SRR4302534.1107162/94‑1 (MQ=60)

                                                                                           TGTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACT    >  SRR4302534.1093637/1‑94 (MQ=60)

                                                                                            GTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTG   <  SRR4302534.6481125/94‑1 (MQ=60)

                                                                                            GTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTG   <  SRR4302534.1509120/94‑1 (MQ=60)

                                                                                            GTCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTG   >  SRR4302534.3188087/1‑94 (MQ=60)

                                                                                             TCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGG  <  SRR4302534.4068959/94‑1 (MQ=60)

ATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGG  >  NC_000913/974782‑974968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`