Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,304	T→C	N169S (AAC→AGC)	solA ←	sarcosine oxidase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,304	0	T	C	99.6%	799.4 / ‑4.6	230	N169S (AAC→AGC)	solA	sarcosine oxidase‑like protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (107/122); total (108/122)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 234 total aligned reads displayed.

CAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAA  >  NC_000913/1119214‑1119394

 aGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGa                                                                                         >  1:1162223/1‑93 (MQ=255)

  gACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGaa                                                                                        <  1:3510219/93‑1 (MQ=255)

   aCAATCGCTTTTTTCGCCTGATACTCGCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAAc                                                                                       <  1:5124084/93‑1 (MQ=255)

     aaTCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAg                                                                                     >  2:769231/1‑93 (MQ=255)

             ttttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGcac                                                                             >  2:5805546/1‑93 (MQ=255)

             ttttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGcac                                                                             <  1:1491066/93‑1 (MQ=255)

               ttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAg                                                                           >  1:1087447/1‑93 (MQ=255)

                tCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGc                                                                          <  2:4702995/93‑1 (MQ=255)

                tCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGc                                                                          >  2:1312093/1‑93 (MQ=255)

                 cGCCTGATACTCGCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGcc                                                                         <  1:2445899/93‑1 (MQ=255)

                 cGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCCCACAGcc                                                                         <  1:402128/93‑1 (MQ=255)

                  gCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGccc                                                                        >  2:3523315/1‑93 (MQ=255)

                  gCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGccc                                                                        <  1:1902184/93‑1 (MQ=255)

                   ccTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCg                                                                       <  1:691110/93‑1 (MQ=255)

                    cTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGc                                                                      <  1:2424183/93‑1 (MQ=255)

                    cTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGc                                                                      >  2:3794227/1‑93 (MQ=255)

                    cTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGc                                                                      <  1:1677250/93‑1 (MQ=255)

                    cTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGc                                                                      <  1:2754345/93‑1 (MQ=255)

                    cTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGc                                                                      <  1:542541/93‑1 (MQ=255)

                     tGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGct                                                                     >  1:6303619/1‑93 (MQ=255)

                     tGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGct                                                                     <  1:6289679/93‑1 (MQ=255)

                      gATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGctt                                                                    >  2:2501458/1‑93 (MQ=255)

                      gATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGctt                                                                    <  2:3014680/93‑1 (MQ=255)

                         aCTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttcct                                                                 <  1:3353321/93‑1 (MQ=255)

                          cTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttcctt                                                                <  1:3660076/93‑1 (MQ=255)

                          cTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttcctt                                                                <  2:8738/93‑1 (MQ=255)

                          cTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttcctt                                                                >  2:5467995/1‑93 (MQ=255)

                          cTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttcctt                                                                >  2:2891988/1‑93 (MQ=255)

                           tCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttccttc                                                               <  1:465616/93‑1 (MQ=255)

                           tCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttccttc                                                               <  2:6312576/93‑1 (MQ=255)

                           tCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGcttccttc                                                               >  2:4406509/1‑93 (MQ=255)

                            cACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCg                                                              >  2:4602294/1‑93 (MQ=255)

                              ccGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGcc                                                            <  2:149526/93‑1 (MQ=255)

                                 tCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGAGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGt                                                         <  2:5446302/93‑1 (MQ=255)

                                  cAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGtt                                                        >  2:4075925/1‑93 (MQ=255)

                                  cAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGtt                                                        >  2:594473/1‑93 (MQ=255)

                                  cAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGtt                                                        >  2:3587560/1‑93 (MQ=255)

                                  cAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGtt                                                        <  1:4749305/93‑1 (MQ=255)

                                   aGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTg                                                       >  1:1366447/1‑93 (MQ=255)

                                    gCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGGTTCCTTCGCCAGTTgg                                                      >  1:4221884/1‑93 (MQ=255)

                                      cGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGAt                                                    <  2:3320447/93‑1 (MQ=255)

                                       gTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATc                                                   <  1:66293/93‑1 (MQ=255)

                                         ttCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCa                                                 <  1:6015664/93‑1 (MQ=255)

                                         ttCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCa                                                 <  2:60976/93‑1 (MQ=255)

                                          tCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAg                                                >  1:3952064/1‑93 (MQ=255)

                                            aaTAGTTACGCCATCATCGTCCTGACGAATTGGGGGGACCCGGCCGCGGAAAAGGTGCGCACACCCCGCCTACTTCGCCCGCTGGATCCacgg                                              >  2:3233539/1‑90 (MQ=255)

                                            aaTAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGt                                              <  1:2569337/93‑1 (MQ=255)

                                            aaTAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGt                                              >  1:5760040/1‑93 (MQ=255)

                                            aaTAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGt                                              <  1:1384326/93‑1 (MQ=255)

                                              tAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGttt                                            <  1:260887/93‑1 (MQ=255)

                                              tAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGttt                                            <  2:697747/93‑1 (MQ=255)

                                               aGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGtttt                                           <  1:3435449/93‑1 (MQ=255)

                                               aGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGtttt                                           <  1:170412/93‑1 (MQ=255)

                                                gTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTa                                          >  1:1001963/1‑93 (MQ=255)

                                                  tACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAAt                                        <  2:610884/93‑1 (MQ=255)

                                                  tACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGGTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAAt                                        >  1:5773461/1‑93 (MQ=255)

                                                     gCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGc                                     <  1:977295/93‑1 (MQ=255)

                                                       catcatCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCTAGGTTTTAATCGCCa                                   <  2:1215442/93‑1 (MQ=255)

                                                       catcatCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCa                                   <  1:5607357/93‑1 (MQ=255)

                                                       catcatCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCa                                   <  1:2988222/93‑1 (MQ=255)

                                                       catcatCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCa                                   >  2:5487947/1‑93 (MQ=255)

                                                        atcatcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAg                                  >  1:6122545/1‑93 (MQ=255)

                                                         tcatcGTCATGACGAATTGCGGTGACCGTGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGt                                 <  2:220008/93‑1 (MQ=255)

                                                         tcatcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGt                                 >  2:5265397/1‑93 (MQ=255)

                                                         tcatcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGt                                 <  1:5746165/93‑1 (MQ=255)

                                                         tcatcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGt                                 >  1:2213056/1‑93 (MQ=255)

                                                         tcatcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGt                                 <  2:5345856/93‑1 (MQ=255)

                                                         tcatcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGt                                 <  1:6390024/93‑1 (MQ=255)

                                                         tcatcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGt                                 <  2:1805382/93‑1 (MQ=255)

                                                          catcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGtt                                >  1:500911/1‑93 (MQ=255)

                                                           atcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTc                               >  2:208749/1‑93 (MQ=255)

                                                           atcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTc                               <  1:554718/93‑1 (MQ=255)

                                                            tcGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCg                              >  1:4573755/1‑93 (MQ=255)

                                                             cGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGc                             <  1:6060538/93‑1 (MQ=255)

                                                              gTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCt                            <  2:979227/93‑1 (MQ=255)

                                                              gTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCt                            <  2:1070333/93‑1 (MQ=255)

                                                                cATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTgc                          <  1:2666854/93‑1 (MQ=255)

                                                                 aTGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTgcg                         >  2:6186774/1‑93 (MQ=255)

                                                                  tGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTgcgc                        <  2:44121/93‑1 (MQ=255)

                                                                   gACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCa                       >  1:1845574/1‑93 (MQ=255)

                                                                    aCGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaa                      <  1:2396587/93‑1 (MQ=255)

                                                                      gAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaaca                    >  2:2831129/1‑91 (MQ=255)

                                                                        aTTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaaaaaa                  >  2:5145039/1‑93 (MQ=255)

                                                                        aTTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaaaaaa                  >  2:2237039/1‑93 (MQ=255)

                                                                        aTTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaaaaaa                  <  2:1651805/93‑1 (MQ=255)

                                                                        aTTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaaaaaa                  <  1:6268236/93‑1 (MQ=255)

                                                                         ttGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAAc                 <  2:2351338/93‑1 (MQ=255)

                                                                          tGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAAcc                <  2:2638704/93‑1 (MQ=255)

                                                                          tGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAAcc                <  2:741889/93‑1 (MQ=255)

                                                                           gCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCg               <  2:777617/93‑1 (MQ=255)

                                                                              gTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGaa            <  1:2785454/93‑1 (MQ=255)

                                                                              gTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGaa            <  1:2044714/93‑1 (MQ=255)

                                                                                gACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATc          <  2:4950542/93‑1 (MQ=255)

                                                                                 aCCGGGCAGCTGAACTGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCa         >  1:3736445/1‑93 (MQ=255)

                                                                                 aCCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACAGGAATCa         >  1:4854262/1‑93 (MQ=255)

                                                                                   cGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGt       <  1:882694/93‑1 (MQ=255)

                                                                                    gggCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGtt      >  2:560725/1‑93 (MQ=255)

                                                                                    gggCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGtt      <  2:3136122/93‑1 (MQ=255)

                                                                                     ggCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGttt     <  1:1314494/93‑1 (MQ=255)

                                                                                       cAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCa   <  2:5731441/93‑1 (MQ=255)

CAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAA  >  NC_000913/1119214‑1119394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATA  >  NC_000913/1119211‑1119397

GCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGC                                                                                               >  SRR4302534.5704633/1‑94 (MQ=60)

 CGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCT                                                                                              >  SRR4302534.3985800/1‑94 (MQ=60)

 CGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCT                                                                                              >  SRR4302534.5826024/1‑94 (MQ=60)

   CAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGA                                                                                            >  SRR4302534.1192722/1‑94 (MQ=60)

   CAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGA                                                                                            <  SRR4302534.5740376/94‑1 (MQ=60)

    AGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAA                                                                                           >  SRR4302534.2682581/1‑94 (MQ=60)

     GACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAAC                                                                                          <  SRR4302534.3604651/94‑1 (MQ=60)

     GACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAAC                                                                                          <  SRR4302534.3528954/94‑1 (MQ=60)

      ACAATCGCTTTTTTCGCCTGATACTCGCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACA                                                                                         <  SRR4302534.5262441/94‑1 (MQ=60)

        AATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGT                                                                                       <  SRR4302534.3927227/94‑1 (MQ=60)

         ATGGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTT                                                                                      >  SRR4302534.6348637/1‑94 (MQ=60)

           CGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGC                                                                                    <  SRR4302534.1389327/94‑1 (MQ=60)

            GCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCG                                                                                   <  SRR4302534.4885883/94‑1 (MQ=60)

            GCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCG                                                                                   >  SRR4302534.5886749/1‑94 (MQ=60)

              TTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCA                                                                                 <  SRR4302534.3778871/94‑1 (MQ=60)

               TTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCAC                                                                                >  SRR4302534.108003/1‑94 (MQ=60)

                TTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACA                                                                               <  SRR4302534.1530284/94‑1 (MQ=60)

                TTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACA                                                                               <  SRR4302534.2534345/94‑1 (MQ=60)

                 TTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAG                                                                              >  SRR4302534.1115735/1‑94 (MQ=60)

                   TCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCC                                                                            >  SRR4302534.3455410/1‑94 (MQ=60)

                    CGCCTGATACTCGCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCC                                                                           <  SRR4302534.2510904/94‑1 (MQ=60)

                    CGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCC                                                                           >  SRR4302534.55876/1‑94 (MQ=60)

                    CGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCCCACAGCCC                                                                           <  SRR4302534.412407/94‑1 (MQ=60)

                     GCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCG                                                                          >  SRR4302534.5447594/1‑94 (MQ=60)

                     GCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCG                                                                          <  SRR4302534.1951797/94‑1 (MQ=60)

                      CCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGC                                                                         <  SRR4302534.708024/94‑1 (MQ=60)

                       CTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCT                                                                        <  SRR4302534.1720892/94‑1 (MQ=60)

                       CTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCT                                                                        <  SRR4302534.555953/94‑1 (MQ=60)

                       CTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCT                                                                        <  SRR4302534.2826897/94‑1 (MQ=60)

                       CTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCT                                                                        >  SRR4302534.6474083/1‑94 (MQ=60)

                       CTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCT                                                                        <  SRR4302534.2488647/94‑1 (MQ=60)

                        TGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAGCAGTTGCGCACAGCCCGCTT                                                                       <  SRR4302534.6459849/94‑1 (MQ=60)

                        TGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTT                                                                       <  SRR4302534.6459850/94‑1 (MQ=60)

                          ATACTCACCGTAACCCGTTTCAATAATAACGCCATCATCGTCATGACGAATTGCGGTGTCTGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCC                                                                     <  SRR4302534.188483/94‑1 (MQ=60)

                            ACTCCCCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTT                                                                   <  SRR4302534.2502303/94‑1 (MQ=60)

                            ACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTT                                                                   <  SRR4302534.3443130/94‑1 (MQ=60)

                            ACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTT                                                                   <  SRR4302534.2956183/94‑1 (MQ=60)

                            ACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTT                                                                   <  SRR4302534.978819/94‑1 (MQ=60)

                             CTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTC                                                                  <  SRR4302534.3758446/94‑1 (MQ=60)

                              TCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCG                                                                 <  SRR4302534.1718176/94‑1 (MQ=60)

                              TCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCG                                                                 <  SRR4302534.477378/94‑1 (MQ=60)

                               CACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGC                                                                >  SRR4302534.2256192/1‑94 (MQ=60)

                                ACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCC                                                               >  SRR4302534.6482389/1‑94 (MQ=60)

                                ACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCC                                                               <  SRR4302534.1276132/94‑1 (MQ=60)

                                ACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCC                                                               >  SRR4302534.5069040/1‑94 (MQ=60)

                                   GTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGT                                                            >  SRR4302534.1166172/1‑94 (MQ=60)

                                     CAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCCGTTG                                                          >  SRR4302534.5943436/1‑94 (MQ=60)

                                     CAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTG                                                          >  SRR4302534.1402434/1‑94 (MQ=60)

                                     CAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTG                                                          <  SRR4302534.645509/94‑1 (MQ=60)

                                     CAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTG                                                          <  SRR4302534.4878218/94‑1 (MQ=60)

                                      AGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGGTTCCTTCGCCAGTTGG                                                         >  SRR4302534.4335694/1‑94 (MQ=60)

                                      AGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGG                                                         >  SRR4302534.101383/1‑94 (MQ=60)

                                       GCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGA                                                        >  SRR4302534.2490308/1‑94 (MQ=60)

                                        CCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGAT                                                       >  SRR4302534.1404182/1‑94 (MQ=60)

                                         CGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATC                                                      >  SRR4302534.3424913/1‑94 (MQ=60)

                                         CGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATC                                                      >  SRR4302534.4747910/1‑94 (MQ=60)

                                         CGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATC                                                      >  SRR4302534.6206807/1‑94 (MQ=60)

                                         CGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATC                                                      <  SRR4302534.5152187/94‑1 (MQ=60)

                                         CGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATC                                                      >  SRR4302534.2782087/1‑94 (MQ=60)

                                          GTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCC                                                     <  SRR4302534.68094/94‑1 (MQ=60)

                                          GTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCC                                                     >  SRR4302534.1433575/1‑94 (MQ=60)

                                          GTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCC                                                     <  SRR4302534.2338833/94‑1 (MQ=60)

                                           TTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCA                                                    <  SRR4302534.3865455/94‑1 (MQ=60)

                                            TTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAG                                                   <  SRR4302534.6179125/94‑1 (MQ=60)

                                            TTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAG                                                   >  SRR4302534.3601929/1‑94 (MQ=60)

                                            TTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAG                                                   >  SRR4302534.4057854/1‑94 (MQ=60)

                                            TTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAG                                                   >  SRR4302534.6258503/1‑94 (MQ=60)

                                              CAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACTGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGT                                                 >  SRR4302534.5916427/1‑94 (MQ=60)

                                              CAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGT                                                 >  SRR4302534.5916284/1‑94 (MQ=60)

                                               AATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTT                                                >  SRR4302534.1868584/1‑94 (MQ=60)

                                               AATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTT                                                <  SRR4302534.1420771/94‑1 (MQ=60)

                                               AATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTT                                                <  SRR4302534.2637419/94‑1 (MQ=60)

                                                ATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTT                                               <  SRR4302534.2985810/94‑1 (MQ=60)

                                                ATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTT                                               >  SRR4302534.282643/1‑94 (MQ=60)

                                                ATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTT                                               <  SRR4302534.120107/94‑1 (MQ=60)

                                                 TAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTT                                              <  SRR4302534.267668/94‑1 (MQ=60)

                                                  AGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTA                                             <  SRR4302534.174915/94‑1 (MQ=60)

                                                  AGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTA                                             <  SRR4302534.1431371/94‑1 (MQ=60)

                                                  AGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTA                                             >  SRR4302534.1027652/1‑94 (MQ=60)

                                                  AGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTA                                             <  SRR4302534.3527768/94‑1 (MQ=60)

                                                    TTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGGTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAAT                                           >  SRR4302534.5930083/1‑94 (MQ=60)

                                                      ACGCAATCATCGTCATGACGAACTGCGGTGACTGGACGGCGGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCG                                         <  SRR4302534.3127478/94‑1 (MQ=60)

                                                        GCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCC                                       >  SRR4302534.4087999/1‑94 (MQ=60)

                                                        GCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCC                                       <  SRR4302534.6538892/94‑1 (MQ=60)

                                                        GCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCC                                       <  SRR4302534.1002239/94‑1 (MQ=60)

                                                         CCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTTCTCGCCA                                      >  SRR4302534.723366/1‑94 (MQ=60)

                                                         CCATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCA                                      >  SRR4302534.2029715/1‑94 (MQ=60)

                                                          CATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAG                                     >  SRR4302534.2190972/1‑94 (MQ=60)

                                                          CATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAG                                     <  SRR4302534.5759033/94‑1 (MQ=60)

                                                          CATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAG                                     <  SRR4302534.4685161/94‑1 (MQ=60)

                                                          CATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAG                                     <  SRR4302534.3066358/94‑1 (MQ=60)

                                                          CATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAG                                     >  SRR4302534.6289083/1‑94 (MQ=60)

                                                           ATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGT                                    >  SRR4302534.180052/1‑94 (MQ=60)

                                                           ATCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGT                                    >  SRR4302534.2271666/1‑94 (MQ=60)

                                                            TCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTT                                   <  SRR4302534.5901967/94‑1 (MQ=60)

                                                            TCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTT                                   >  SRR4302534.513387/1‑94 (MQ=60)

                                                            TCATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTT                                   <  SRR4302534.6562618/94‑1 (MQ=60)

                                                             CATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCTCCAGTTC                                  <  SRR4302534.5505820/94‑1 (MQ=60)

                                                             CATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTC                                  <  SRR4302534.6154311/94‑1 (MQ=60)

                                                              ATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCG                                 <  SRR4302534.568389/94‑1 (MQ=60)

                                                              ATCGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCG                                 >  SRR4302534.4697790/1‑94 (MQ=60)

                                                                CGTCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCT                               <  SRR4302534.6225332/94‑1 (MQ=60)

                                                                  TCATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGC                             >  SRR4302534.684688/1‑94 (MQ=60)

                                                                   CATGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCG                            <  SRR4302534.2737347/94‑1 (MQ=60)

                                                                     TGACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCA                          >  SRR4302534.1893582/1‑94 (MQ=60)

                                                                      GACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAA                         >  SRR4302534.2482139/1‑94 (MQ=60)

                                                                       ACGAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAA                        <  SRR4302534.2460266/94‑1 (MQ=60)

                                                                         GAATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAA                      >  SRR4302534.4605526/1‑94 (MQ=60)

                                                                           ATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAAC                    >  SRR4302534.1737106/1‑94 (MQ=60)

                                                                           ATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAAC                    <  SRR4302534.6437973/94‑1 (MQ=60)

                                                                           ATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAAC                    <  SRR4302534.2696361/94‑1 (MQ=60)

                                                                           ATTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTAAATCGCCAGTTCGCTGCGCAAAAAAC                    <  SRR4302534.3691303/94‑1 (MQ=60)

                                                                            TTGCGGTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACC                   <  SRR4302534.5832413/94‑1 (MQ=60)

                                                                                 GTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAAT              >  SRR4302534.6310649/1‑94 (MQ=60)

                                                                                 GTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAAT              <  SRR4302534.2098160/94‑1 (MQ=60)

                                                                                 GTGACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAAT              <  SRR4302534.2858715/94‑1 (MQ=60)

                                                                                   GACCGGGCAGCTGAACTGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCA            >  SRR4302534.3836812/1‑94 (MQ=60)

                                                                                   GACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCA            <  SRR4302534.5904559/94‑1 (MQ=60)

                                                                                   GACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCA            >  SRR4302534.2650040/1‑94 (MQ=60)

                                                                                   GACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACAGGAATCA            >  SRR4302534.4985964/1‑94 (MQ=60)

                                                                                    ACCGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGCTCGCTGCGCAAAAAACCGGAATCAG           <  SRR4302534.1920271/94‑1 (MQ=60)

                                                                                      CGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTT         >  SRR4302534.4800837/1‑94 (MQ=60)

                                                                                      CGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTT         <  SRR4302534.905026/94‑1 (MQ=60)

                                                                                      CGGGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATAAGTT         >  SRR4302534.4965346/1‑94 (MQ=60)

                                                                                        GGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTC       <  SRR4302534.1349156/94‑1 (MQ=60)

                                                                                        GGCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTC       <  SRR4302534.4330943/94‑1 (MQ=60)

                                                                                         GCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCA      <  SRR4302534.662011/94‑1 (MQ=60)

                                                                                         GCAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCA      >  SRR4302534.690548/1‑94 (MQ=60)

                                                                                          CAGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAA     >  SRR4302534.2528509/1‑94 (MQ=60)

                                                                                           AGCTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAA    >  SRR4302534.6500932/1‑94 (MQ=60)

                                                                                             CTGAACAGTTGCGCACAGCCAGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATA  >  SRR4302534.3361860/1‑94 (MQ=60)

GCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATA  >  NC_000913/1119211‑1119397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`