Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,130,725 A→G N22S (AAC→AGC)  flgC → FlgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,130,7250AG100.0% 743.7 / NA 213N22S (AAC→AGC) flgCFlgC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (92/121);  total (92/121)

Only 100 of 215 total aligned reads displayed.

ACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTA  >  NC_000913/1130635‑1130814
                                                                                          |                                                                                         
aCGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCg                                                                                         >  1:5848025/1‑93 (MQ=255)
aCGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCg                                                                                         >  2:167147/1‑93 (MQ=255)
aCGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCg                                                                                         <  1:2286968/93‑1 (MQ=255)
 cGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGt                                                                                        <  2:4665853/93‑1 (MQ=255)
 cGTTCTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGt                                                                                        <  2:2651765/93‑1 (MQ=255)
   ttttACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTgg                                                                                      <  2:3081163/93‑1 (MQ=255)
    tttACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTggc                                                                                     <  1:2836971/93‑1 (MQ=255)
    tttACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTggc                                                                                     >  2:3153566/1‑93 (MQ=255)
       aCAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTggcggc                                                                                  >  1:1229535/1‑93 (MQ=255)
       aCAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTggccgc                                                                                  >  2:2452505/1‑93 (MQ=255)
        cAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGcc                                                                                 >  1:768186/1‑93 (MQ=255)
         agagCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCa                                                                                <  1:1797832/93‑1 (MQ=255)
         agagCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCa                                                                                <  2:5795414/93‑1 (MQ=255)
          gagCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAg                                                                               <  1:4259466/93‑1 (MQ=255)
          gagCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAg                                                                               >  2:696201/1‑93 (MQ=255)
          gagCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAg                                                                               >  2:80640/1‑93 (MQ=255)
           agCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGt                                                                              >  2:3614663/1‑93 (MQ=255)
              ggAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAAt                                                                           <  2:368530/93‑1 (MQ=255)
               gAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATc                                                                          <  2:1049464/93‑1 (MQ=255)
               gAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATc                                                                          <  2:432186/93‑1 (MQ=255)
               gAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATc                                                                          >  1:5294943/1‑93 (MQ=255)
               gAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATc                                                                          >  2:2871742/1‑93 (MQ=255)
                aaaTTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCt                                                                         <  2:346893/93‑1 (MQ=255)
                  aTTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTgg                                                                       >  2:3809253/1‑93 (MQ=255)
                    tAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCg                                                                     <  2:6044103/93‑1 (MQ=255)
                     aaCGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGa                                                                    <  2:738676/93‑1 (MQ=255)
                      aCGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGaa                                                                   >  1:2045545/1‑93 (MQ=255)
                      aCGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGaa                                                                   >  1:5057225/1‑93 (MQ=255)
                       cGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAAt                                                                  >  1:1021775/1‑93 (MQ=255)
                         gATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGc                                                                >  2:1735346/1‑93 (MQ=255)
                         gATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGc                                                                >  1:5168097/1‑93 (MQ=255)
                          gtGGCACTGCTGAATATTTTTGATATCGCCGGGTAGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCTTGGCGGCCAGTAATCTGGCGAATGCt                                                               <  2:5081780/92‑1 (MQ=255)
                           tGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTg                                                              <  2:2487318/93‑1 (MQ=255)
                           tGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTg                                                              <  1:4722045/93‑1 (MQ=255)
                            ggCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGa                                                             <  1:844690/93‑1 (MQ=255)
                            ggCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGa                                                             <  1:2563565/93‑1 (MQ=255)
                             gCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGAt                                                            <  1:3888111/93‑1 (MQ=255)
                             gCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGAt                                                            <  1:6216692/93‑1 (MQ=255)
                              cACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATa                                                           >  1:3987214/1‑93 (MQ=255)
                              cACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATa                                                           >  2:5380273/1‑93 (MQ=255)
                              cACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATa                                                           >  2:2704782/1‑93 (MQ=255)
                                ctgctgAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGc                                                         <  2:2091228/93‑1 (MQ=255)
                                ctgctgAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGc                                                         <  1:3733059/93‑1 (MQ=255)
                                 tgctgAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCg                                                        >  1:6194453/1‑93 (MQ=255)
                                  gctgAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGt                                                       >  1:5700034/1‑93 (MQ=255)
                                  gctgAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGt                                                       <  2:396413/93‑1 (MQ=255)
                                    tgAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGa                                                     >  2:3344952/1‑93 (MQ=255)
                                     gAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGAc                                                    <  1:6086021/93‑1 (MQ=255)
                                     gAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGAc                                                    <  2:4633923/93‑1 (MQ=255)
                                      aaTATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGAcc                                                   >  2:1936978/1‑93 (MQ=255)
                                         atTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCggc                                                >  1:884607/1‑92 (MQ=255)
                                          tttttGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTc                                               <  2:570532/93‑1 (MQ=255)
                                          tttttGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTc                                               >  2:6454018/1‑93 (MQ=255)
                                          ttttgGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTc                                               <  2:5554786/93‑1 (MQ=255)
                                              tGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGa                                           >  1:3361050/1‑93 (MQ=255)
                                               gATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGAt                                          <  2:3067457/93‑1 (MQ=255)
                                                atatCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATg                                         <  2:1171523/93‑1 (MQ=255)
                                                   tCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGAc                                      <  1:4034681/93‑1 (MQ=255)
                                                   tCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGAc                                      >  2:2225832/1‑93 (MQ=255)
                                                    cGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACa                                     <  1:3587908/93‑1 (MQ=255)
                                                     gCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAg                                    >  2:42373/1‑93 (MQ=255)
                                                        gggTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCa                                 >  1:2631246/1‑93 (MQ=255)
                                                          gTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCata                               <  1:1107068/93‑1 (MQ=255)
                                                            cGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATc                             >  1:298467/1‑93 (MQ=255)
                                                            cGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATc                             <  2:1656367/93‑1 (MQ=255)
                                                            cGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATc                             <  1:449310/93‑1 (MQ=255)
                                                             ggCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTGATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCg                            >  1:5487479/1‑93 (MQ=255)
                                                             ggCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCg                            <  1:4407590/93‑1 (MQ=255)
                                                              gCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCgg                           >  1:6410740/1‑93 (MQ=255)
                                                              gCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCgg                           <  1:454419/93‑1 (MQ=255)
                                                              gCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCgg                           <  2:3244775/93‑1 (MQ=255)
                                                              gCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCgg                           <  1:2197049/93‑1 (MQ=255)
                                                               cGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCggg                          <  1:485868/93‑1 (MQ=255)
                                                               cGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCggg                          <  1:54343/93‑1 (MQ=255)
                                                                gTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGc                         >  2:5852327/1‑93 (MQ=255)
                                                                gTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGc                         <  1:3155471/93‑1 (MQ=255)
                                                                gTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGc                         <  1:1143051/93‑1 (MQ=255)
                                                                 ttAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCa                        >  2:1877044/1‑93 (MQ=255)
                                                                 ttAACTGCCCAGCCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCa                        <  2:6108067/93‑1 (MQ=255)
                                                                    aCTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCaaaa                     >  2:6230472/1‑93 (MQ=255)
                                                                     cTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAAc                    <  1:1269896/93‑1 (MQ=255)
                                                                       gCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAg                  <  2:363655/93‑1 (MQ=255)
                                                                       gCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAg                  <  2:6284892/93‑1 (MQ=255)
                                                                       gCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAg                  >  2:2525765/1‑93 (MQ=255)
                                                                         ccAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggt                <  2:2751547/93‑1 (MQ=255)
                                                                         ccAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggg                >  1:4627314/1‑92 (MQ=255)
                                                                          cAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggtg               <  2:4371467/93‑1 (MQ=255)
                                                                          cAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggtg               >  2:3385371/1‑93 (MQ=255)
                                                                           aGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggtgg              >  1:2404427/1‑93 (MQ=255)
                                                                            gTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggtggt             >  1:3852976/1‑93 (MQ=255)
                                                                              cccAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTAt           <  1:1912921/93‑1 (MQ=255)
                                                                                 aGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTcc        <  2:543844/93‑1 (MQ=255)
                                                                                  gcgcCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCCTATCGGGCAAAACAGGTGGTATTCCa       >  1:5200220/1‑93 (MQ=255)
                                                                                  gcgcCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCa       >  1:5662803/1‑93 (MQ=255)
                                                                                  gcgcCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCa       <  1:4086867/93‑1 (MQ=255)
                                                                                   cgcCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAg      <  1:783781/93‑1 (MQ=255)
                                                                                   cgcCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAg      >  1:4887792/1‑93 (MQ=255)
                                                                                    gcCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGCGGTATTCGAgg     >  1:134212/1‑93 (MQ=255)
                                                                                     ccTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGt    >  1:1481498/1‑93 (MQ=255)
                                                                                      cTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGtt   <  1:6113535/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTA  >  NC_000913/1130635‑1130814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGC  >  NC_000913/1130632‑1130818
                                                                                             |                                                                                             
TGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAG                                                                                               <  SRR4302534.943210/94‑1 (MQ=60)
TGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAG                                                                                               >  SRR4302534.686595/1‑94 (MQ=60)
TGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAG                                                                                               >  SRR4302534.6542895/1‑94 (MQ=60)
TGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAG                                                                                               >  SRR4302534.1578039/1‑94 (MQ=60)
  AACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCG                                                                                             >  SRR4302534.6006800/1‑94 (MQ=60)
  AACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCG                                                                                             >  SRR4302534.2768015/1‑94 (MQ=60)
   ACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGT                                                                                            <  SRR4302534.4541790/94‑1 (MQ=60)
   ACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGT                                                                                            <  SRR4302534.2347661/94‑1 (MQ=60)
    CGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTG                                                                                           <  SRR4302534.4393972/94‑1 (MQ=60)
    CGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTG                                                                                           >  SRR4302534.854464/1‑94 (MQ=60)
      TTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGC                                                                                         <  SRR4302534.4752559/94‑1 (MQ=60)
       TTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCG                                                                                        <  SRR4302534.645243/94‑1 (MQ=60)
       TTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCG                                                                                        <  SRR4302534.2911514/94‑1 (MQ=60)
         TACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGC                                                                                      >  SRR4302534.1261904/1‑94 (MQ=60)
          ACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCC                                                                                     >  SRR4302534.787282/1‑94 (MQ=60)
          ACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCC                                                                                     <  SRR4302534.3989721/94‑1 (MQ=60)
           CAGAGCGGCAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCA                                                                                    >  SRR4302534.128791/1‑94 (MQ=60)
            AGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAG                                                                                   <  SRR4302534.1844520/94‑1 (MQ=60)
             GAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGT                                                                                  <  SRR4302534.4374475/94‑1 (MQ=60)
                 GGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATC                                                                              <  SRR4302534.4659784/94‑1 (MQ=60)
                 GGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATC                                                                              >  SRR4302534.6263535/1‑94 (MQ=60)
                 GGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATC                                                                              >  SRR4302534.5437856/1‑94 (MQ=60)
                     ATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGC                                                                          <  SRR4302534.3540028/94‑1 (MQ=60)
                       TAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGA                                                                        >  SRR4302534.2089138/1‑94 (MQ=60)
                        AACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAA                                                                       >  SRR4302534.3294852/1‑94 (MQ=60)
                        AACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAA                                                                       >  SRR4302534.5193824/1‑94 (MQ=60)
                        AACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAA                                                                       >  SRR4302534.2099012/1‑94 (MQ=60)
                        AACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAA                                                                       <  SRR4302534.1724040/94‑1 (MQ=60)
                         ACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAAT                                                                      >  SRR4302534.1048089/1‑94 (MQ=60)
                           GGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGC                                                                    >  SRR4302534.2459360/1‑94 (MQ=60)
                           GGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGC                                                                    >  SRR4302534.5307399/1‑94 (MQ=60)
                            GATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCT                                                                   >  SRR4302534.4248898/1‑94 (MQ=60)
                              TGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGA                                                                 <  SRR4302534.2610135/94‑1 (MQ=60)
                              TGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGA                                                                 <  SRR4302534.4850299/94‑1 (MQ=60)
                               GGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGAT                                                                <  SRR4302534.2631505/94‑1 (MQ=60)
                               GGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGAT                                                                <  SRR4302534.865977/94‑1 (MQ=60)
                                GCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATA                                                               <  SRR4302534.3992484/94‑1 (MQ=60)
                                GCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATA                                                               >  SRR4302534.4093898/1‑94 (MQ=60)
                                GCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATA                                                               <  SRR4302534.5247608/94‑1 (MQ=60)
                                GCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATA                                                               <  SRR4302534.6385589/94‑1 (MQ=60)
                                 CACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAG                                                              <  SRR4302534.516461/94‑1 (MQ=60)
                                  ACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGC                                                             <  SRR4302534.5729376/94‑1 (MQ=60)
                                   CTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCG                                                            >  SRR4302534.6363002/1‑94 (MQ=60)
                                   CTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCG                                                            <  SRR4302534.3833357/94‑1 (MQ=60)
                                    TGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGT                                                           >  SRR4302534.5854443/1‑94 (MQ=60)
                                    TGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGT                                                           <  SRR4302534.561177/94‑1 (MQ=60)
                                    TGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGT                                                           >  SRR4302534.2317558/1‑94 (MQ=60)
                                     GCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTG                                                          <  SRR4302534.4037170/94‑1 (MQ=60)
                                       TGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGAC                                                        >  SRR4302534.441670/1‑94 (MQ=60)
                                        GAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACC                                                       <  SRR4302534.6251505/94‑1 (MQ=60)
                                        GAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGGCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACC                                                       <  SRR4302534.6560269/94‑1 (MQ=60)
                                          ATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGG                                                     >  SRR4302534.2636984/1‑94 (MQ=60)
                                           TATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGC                                                    >  SRR4302534.906993/1‑94 (MQ=60)
                                             TTTTTGATATCGCCGGGTCGGCTTTAACTGCCCAGTGCCAGCGCCTGAGCGTGGCGGCTAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCC                                                  <  SRR4302534.5938145/94‑1 (MQ=60)
                                             TTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCC                                                  <  SRR4302534.635212/94‑1 (MQ=60)
                                              TTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCC                                                 <  SRR4302534.3793217/94‑1 (MQ=60)
                                              TTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCC                                                 <  SRR4302534.4191604/94‑1 (MQ=60)
                                               tagatTATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCG                                                <  SRR4302534.5540902/89‑1 (MQ=60)
                                               TTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCG                                                <  SRR4302534.866065/94‑1 (MQ=60)
                                               TTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCG                                                <  SRR4302534.5335798/94‑1 (MQ=60)
                                                TTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGA                                               <  SRR4302534.5052004/94‑1 (MQ=60)
                                                TTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGA                                               >  SRR4302534.3451075/1‑94 (MQ=60)
                                                 TGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGAT                                              >  SRR4302534.797438/1‑94 (MQ=60)
                                                   ATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGG                                            >  SRR4302534.1313567/1‑94 (MQ=60)
                                                    AATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGA                                           <  SRR4302534.1878415/94‑1 (MQ=60)
                                                      TCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACA                                         <  SRR4302534.4142842/94‑1 (MQ=60)
                                                      TCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACA                                         <  SRR4302534.4126513/94‑1 (MQ=60)
                                                       CGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAG                                        <  SRR4302534.483288/94‑1 (MQ=60)
                                                       CGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAG                                        <  SRR4302534.3684408/94‑1 (MQ=60)
                                                         CCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCC                                      >  SRR4302534.6348918/1‑94 (MQ=60)
                                                          CGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCA                                     <  SRR4302534.4997617/94‑1 (MQ=60)
                                                          CGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCA                                     >  SRR4302534.2700864/1‑94 (MQ=60)
                                                          CGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCA                                     >  SRR4302534.1264975/1‑94 (MQ=60)
                                                           GGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTACCGACGGACAGCCAT                                    >  SRR4302534.2640316/1‑94 (MQ=60)
                                                             GTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATAT                                  <  SRR4302534.1135921/94‑1 (MQ=60)
                                                             GTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATAT                                  >  SRR4302534.4922746/1‑94 (MQ=60)
                                                              TCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATC                                 >  SRR4302534.306182/1‑94 (MQ=60)
                                                               CGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTGATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCG                                >  SRR4302534.5635477/1‑94 (MQ=60)
                                                               CGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCG                                <  SRR4302534.460689/94‑1 (MQ=60)
                                                               CGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCG                                <  SRR4302534.3121900/94‑1 (MQ=60)
                                                                GGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGG                               <  SRR4302534.3923422/94‑1 (MQ=60)
                                                                GGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGG                               >  SRR4302534.1147478/1‑94 (MQ=60)
                                                                GGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGG                               >  SRR4302534.6583728/1‑94 (MQ=60)
                                                                GGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGG                               <  SRR4302534.4526542/94‑1 (MQ=60)
                                                                 GCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGG                              <  SRR4302534.2255179/94‑1 (MQ=60)
                                                                 GCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGG                              <  SRR4302534.465920/94‑1 (MQ=60)
                                                                 GCCTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGG                              <  SRR4302534.1411514/94‑1 (MQ=60)
                                                                  CGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGC                             <  SRR4302534.55814/94‑1 (MQ=60)
                                                                  CGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGC                             <  SRR4302534.498030/94‑1 (MQ=60)
                                                                   GTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCA                            <  SRR4302534.1172979/94‑1 (MQ=60)
                                                                   GTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCA                            <  SRR4302534.3238573/94‑1 (MQ=60)
                                                                        CTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACA                       <  SRR4302534.1303347/94‑1 (MQ=60)
                                                                           CCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGG                    >  SRR4302534.4752865/1‑94 (MQ=60)
                                                                            CCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTG                   <  SRR4302534.2099635/94‑1 (MQ=60)
                                                                            CCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTG                   <  SRR4302534.1212265/94‑1 (MQ=60)
                                                                             CAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGG                  <  SRR4302534.1126257/94‑1 (MQ=60)
                                                                             CAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGG                  >  SRR4302534.2468329/1‑94 (MQ=60)
                                                                              AGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGT                 <  SRR4302534.177691/94‑1 (MQ=60)
                                                                              AGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGT                 >  SRR4302534.3956420/1‑94 (MQ=60)
                                                                                 CCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATT              <  SRR4302534.1962853/94‑1 (MQ=60)
                                                                                   CAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCC            <  SRR4302534.5821057/94‑1 (MQ=60)
                                                                                    AGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCCTATCGGGCAAAACAGGTGGTATTCCA           >  SRR4302534.5340502/1‑94 (MQ=60)
                                                                                    AGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCA           >  SRR4302534.5816108/1‑94 (MQ=60)
                                                                                     GCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAG          >  SRR4302534.5020334/1‑94 (MQ=60)
                                                                                     GCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAG          <  SRR4302534.4196613/94‑1 (MQ=60)
                                                                                      CGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGG         >  SRR4302534.5873743/1‑94 (MQ=60)
                                                                                      CGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGG         <  SRR4302534.803333/94‑1 (MQ=60)
                                                                                      CGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGCGGTATTCGAGG         >  SRR4302534.137744/1‑94 (MQ=60)
                                                                                       GCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGT        >  SRR4302534.1520459/1‑94 (MQ=60)
                                                                                        CCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTT       <  SRR4302534.759482/94‑1 (MQ=60)
                                                                                        CCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTT       <  SRR4302534.579595/94‑1 (MQ=60)
                                                                                         CTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTGCCGATGGACAGCCATATCGCGCAAAACAGGCGGGATTCCAGGTTA      >  SRR4302534.6042789/1‑94 (MQ=60)
                                                                                         CTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTA      >  SRR4302534.5376335/1‑94 (MQ=60)
                                                                                         CTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTA      >  SRR4302534.3027858/1‑94 (MQ=60)
                                                                                         CTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTA      <  SRR4302534.6279857/94‑1 (MQ=60)
                                                                                            AGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACG   <  SRR4302534.180189/94‑1 (MQ=60)
                                                                                             GCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGC  >  SRR4302534.539700/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGGTATTCCAGGTTAACGC  >  NC_000913/1130632‑1130818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑