Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,184,516 A→G F101S (TTC→TCC)  potA ← ATP‑binding component of spermidine/putrescine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,184,5160AG100.0% 913.0 / NA 260F101S (TTC→TCC) potAATP‑binding component of spermidine/putrescine transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (113/147);  total (113/147)

Only 100 of 277 total aligned reads displayed.

GCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCG  >  NC_000913/1184426‑1184605
                                                                                          |                                                                                         
gCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGgaa                                                                                         <  2:2687941/93‑1 (MQ=255)
gCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGgaa                                                                                         <  2:5035699/93‑1 (MQ=255)
 cTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGgaaa                                                                                        <  1:4955993/93‑1 (MQ=255)
  ttCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAg                                                                                       >  2:1750445/1‑93 (MQ=255)
   tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGACCACGGTCATGTGGGGGGAAAGt                                                                                      <  2:6420504/93‑1 (MQ=255)
     cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGc                                                                                    <  2:4461671/93‑1 (MQ=255)
     cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGc                                                                                    >  2:3340837/1‑93 (MQ=255)
     cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGc                                                                                    <  1:4697897/93‑1 (MQ=255)
         aCGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAg                                                                                >  1:1380699/1‑93 (MQ=255)
         aCGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAg                                                                                >  2:66244/1‑93 (MQ=255)
          cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGc                                                                               <  2:1425082/93‑1 (MQ=255)
          cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGc                                                                               >  2:499501/1‑93 (MQ=255)
          cgcgGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCt                                                                              <  1:4798070/93‑1 (MQ=255)
           gcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCt                                                                              >  2:434223/1‑93 (MQ=255)
            cgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCtt                                                                             <  1:2208875/93‑1 (MQ=255)
            cgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCtt                                                                             >  1:3355175/1‑93 (MQ=255)
             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGGAAGTGCGTAGCttt                                                                            >  1:6309813/1‑93 (MQ=255)
             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCttt                                                                            <  1:4109337/93‑1 (MQ=255)
             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCttt                                                                            <  2:5814704/93‑1 (MQ=255)
              gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTg                                                                           >  2:4648821/1‑93 (MQ=255)
              gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTg                                                                           <  1:919127/93‑1 (MQ=255)
               ggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTgg                                                                          >  2:3884948/1‑93 (MQ=255)
                gCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGa                                                                         <  2:838945/93‑1 (MQ=255)
                  gTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGaaa                                                                       >  1:2790997/1‑93 (MQ=255)
                   tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGaaaa                                                                      <  1:3926468/93‑1 (MQ=255)
                    aaTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAAc                                                                     <  1:5924274/93‑1 (MQ=255)
                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAg                                                                   <  2:309891/93‑1 (MQ=255)
                           gcagcGGGGGTTTTTTGCATGCGGAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTc                                                              <  2:3937216/93‑1 (MQ=255)
                           gcagcGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTc                                                              >  1:6049524/1‑93 (MQ=255)
                             agcGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTcac                                                            <  2:1643660/93‑1 (MQ=255)
                               cGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACAt                                                          <  2:1092237/93‑1 (MQ=255)
                                gggggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                                                         <  2:3186513/93‑1 (MQ=255)
                                gggggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                                                         <  1:1975409/93‑1 (MQ=255)
                                 ggggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                                                        >  1:4530979/1‑93 (MQ=255)
                                 ggggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                                                        >  2:3799197/1‑93 (MQ=255)
                                   ggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                                                      <  2:888652/93‑1 (MQ=255)
                                   ggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                                                      >  2:2124609/1‑93 (MQ=255)
                                    gTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCgg                                                     <  2:5339371/93‑1 (MQ=255)
                                     ttttttGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGt                                                    <  1:6393993/93‑1 (MQ=255)
                                      tttttGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGtt                                                   <  1:5143140/93‑1 (MQ=255)
                                      tttttGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGtt                                                   <  2:3713262/93‑1 (MQ=255)
                                      tcgttGCATGCGCAGCCCAAAGGCCACATTTTCCAACACGGGCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGtt                                                   <  1:1451648/90‑1 (MQ=255)
                                       ttttGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGttt                                                  <  1:3259351/93‑1 (MQ=255)
                                         ttGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTc                                                <  1:1101081/93‑1 (MQ=255)
                                         ttGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTc                                                <  1:5056432/93‑1 (MQ=255)
                                            cATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGAGCGTAGCTTTGGCAAACAGTGTTCACATAGCGGTTTTccgc                                             >  1:1725015/1‑93 (MQ=255)
                                             aTGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc                                            <  1:1564254/93‑1 (MQ=255)
                                             aTGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc                                            >  2:1849302/1‑93 (MQ=255)
                                              tccgcAACCCAAAGGCCACATTTTCGAACGCGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgccg                                           <  2:1950021/91‑1 (MQ=255)
                                                  cAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAAc                                       <  1:584831/93‑1 (MQ=255)
                                                   aaCCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACg                                      >  2:35522/1‑93 (MQ=255)
                                                    aCCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt                                     >  2:86123/1‑93 (MQ=255)
                                                    aCCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt                                     <  1:4874349/93‑1 (MQ=255)
                                                    aCCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTAGGAAAACAGTGTTCACATAGCGG‑TTTCCGCCGGAACGTg                                    <  2:3588068/93‑1 (MQ=255)
                                                      ccAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg                                   <  1:2960820/93‑1 (MQ=255)
                                                       cAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg                                  <  1:6206652/93‑1 (MQ=255)
                                                       cAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg                                  >  2:3265380/1‑93 (MQ=255)
                                                       cAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg                                  >  2:4321521/1‑93 (MQ=255)
                                                       cAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg                                  <  2:2196304/93‑1 (MQ=255)
                                                       cAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg                                  >  2:6332471/1‑93 (MQ=255)
                                                       cAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg                                  >  2:1195338/1‑93 (MQ=255)
                                                        aaaGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGt                                 <  1:4193205/93‑1 (MQ=255)
                                                         aaGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTg                                <  2:5460108/93‑1 (MQ=255)
                                                         aaGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTg                                >  2:6147014/1‑93 (MQ=255)
                                                          aGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGa                               >  1:3117722/1‑93 (MQ=255)
                                                          aGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGGTTTCCGCCGGAACGTGGGTGa                               >  1:2414783/1‑93 (MQ=255)
                                                            gCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATg                             >  2:4810432/1‑93 (MQ=255)
                                                             ccACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGGACGTGGGTGATGt                            >  1:3746947/1‑93 (MQ=255)
                                                             ccACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGt                            <  1:2722913/93‑1 (MQ=255)
                                                              cacaTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTc                           <  1:3475166/93‑1 (MQ=255)
                                                               acaTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTcc                          <  2:941752/93‑1 (MQ=255)
                                                               acaTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTcc                          >  1:3329745/1‑93 (MQ=255)
                                                               acaTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTcc                          >  1:2535012/1‑93 (MQ=255)
                                                                caTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCt                         <  1:6144521/93‑1 (MQ=255)
                                                                caTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCt                         <  2:2062211/93‑1 (MQ=255)
                                                                caTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCt                         <  2:2449732/93‑1 (MQ=255)
                                                                 aTTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTc                        <  2:6048194/93‑1 (MQ=255)
                                                                   tttCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGt                      <  2:2816207/93‑1 (MQ=255)
                                                                      cGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTAt                   <  2:538969/93‑1 (MQ=255)
                                                                       gAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATc                  <  2:5212452/93‑1 (MQ=255)
                                                                       gAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATc                  <  2:4130234/93‑1 (MQ=255)
                                                                        aaCACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTGCTCGTTATcc                 >  2:4072909/1‑93 (MQ=255)
                                                                        aaCACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATcc                 <  1:4921838/93‑1 (MQ=255)
                                                                        aaCACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATcc                 >  1:1011873/1‑93 (MQ=255)
                                                                        aaCACGGTCATGTGGGGGGAAAGTGCGTAGATTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATcc                 >  2:3051962/1‑93 (MQ=255)
                                                                         acacGGTCATGTGGGGGGACAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGGTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCa                >  1:6275372/1‑93 (MQ=255)
                                                                         acacGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCa                <  1:3871775/93‑1 (MQ=255)
                                                                           acGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGc              >  2:6008990/1‑93 (MQ=255)
                                                                               tCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatga          <  1:5478950/93‑1 (MQ=255)
                                                                               tCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatga          <  2:1350995/93‑1 (MQ=255)
                                                                                cATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatgat         <  2:5643892/93‑1 (MQ=255)
                                                                                 aTGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatgatg        >  1:1525402/1‑93 (MQ=255)
                                                                                 aTGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatgatg        >  1:2500533/1‑93 (MQ=255)
                                                                                  tgtgGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGc       <  2:2354080/93‑1 (MQ=255)
                                                                                      ggggGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTcc   >  1:5222141/1‑93 (MQ=255)
                                                                                      ggggGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTcc   <  1:4310672/93‑1 (MQ=255)
                                                                                      ggggGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATACAGCATGATGCGTcc   >  2:2620019/1‑93 (MQ=255)
                                                                                       ggggAAAGTGCGTAGCTTTGGAAAACAGTGTTCAGATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCg  <  2:3499885/93‑1 (MQ=255)
                                                                                       ggggAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCg  <  1:1768104/93‑1 (MQ=255)
                                                                                       ggggAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCg  >  2:1869950/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCG  >  NC_000913/1184426‑1184605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAAT  >  NC_000913/1184423‑1184609
                                                                                             |                                                                                             
AGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGACCACGGTCATGTGGGGGG                                                                                               >  SRR4302534.1866638/1‑94 (MQ=60)
AGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGG                                                                                               >  SRR4302534.4210191/1‑94 (MQ=60)
AGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGCTTTTTGGATGCGCAACCCAAAGGGCACACTTTCGAACACGGTCATGTGGGGGG                                                                                               >  SRR4302534.5320725/1‑94 (MQ=60)
    CTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAG                                                                                           >  SRR4302534.862778/1‑94 (MQ=60)
    CTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAG                                                                                           >  SRR4302534.5172073/1‑94 (MQ=60)
    CTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAG                                                                                           <  SRR4302534.5090207/94‑1 (MQ=60)
     TTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGT                                                                                          >  SRR4302534.5748092/1‑94 (MQ=60)
       CCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGC                                                                                        >  SRR4302534.1884677/1‑94 (MQ=60)
       CCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGC                                                                                        >  SRR4302534.5007465/1‑94 (MQ=60)
        CATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCG                                                                                       <  SRR4302534.4825413/94‑1 (MQ=60)
          TCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTA                                                                                     <  SRR4302534.5678787/94‑1 (MQ=60)
           CACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAG                                                                                    <  SRR4302534.6462602/94‑1 (MQ=60)
           CACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAG                                                                                    >  SRR4302534.1417056/1‑94 (MQ=60)
            ACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGC                                                                                   >  SRR4302534.3238554/1‑94 (MQ=60)
             CGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCT                                                                                  <  SRR4302534.1170747/94‑1 (MQ=60)
             CGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTT                                                                                 <  SRR4302534.4928349/94‑1 (MQ=60)
              GCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTT                                                                                 >  SRR4302534.1567586/1‑94 (MQ=60)
              GCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTT                                                                                 >  SRR4302534.3445032/1‑94 (MQ=60)
              GCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTT                                                                                 <  SRR4302534.6124723/94‑1 (MQ=60)
               CGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGGAAGTGCGTAGCTTT                                                                                >  SRR4302534.6480405/1‑94 (MQ=60)
               CGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTT                                                                                <  SRR4302534.2267355/94‑1 (MQ=60)
               CGGGGCGTAATTTCAGCAGCGGGGGATTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTT                                                                                >  SRR4302534.4084462/1‑94 (MQ=60)
                GGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTG                                                                               <  SRR4302534.4262186/94‑1 (MQ=60)
                GGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTG                                                                               <  SRR4302534.4219870/94‑1 (MQ=60)
                 GGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGG                                                                              <  SRR4302534.942532/94‑1 (MQ=60)
                    CGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAA                                                                           >  SRR4302534.2864398/1‑94 (MQ=60)
                      TAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAAC                                                                         <  SRR4302534.4031655/94‑1 (MQ=60)
                      TAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAAC                                                                         >  SRR4302534.4853663/1‑94 (MQ=60)
                       AATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACA                                                                        <  SRR4302534.6085134/94‑1 (MQ=60)
                        ATTTCAGCATCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAG                                                                       <  SRR4302534.6070215/94‑1 (MQ=60)
                         TTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGT                                                                      <  SRR4302534.4940075/94‑1 (MQ=60)
                         TTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGG                                                                      >  SRR4302534.2284586/1‑94 (MQ=60)
                          TTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTG                                                                     <  SRR4302534.4696579/94‑1 (MQ=60)
                          TTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTG                                                                     <  SRR4302534.5352852/94‑1 (MQ=60)
                             AGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTC                                                                  >  SRR4302534.2369847/1‑94 (MQ=60)
                             AGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTC                                                                  >  SRR4302534.6213998/1‑94 (MQ=60)
                              GCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCA                                                                 >  SRR4302534.283531/1‑94 (MQ=60)
                                AGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACA                                                               <  SRR4302534.4232066/94‑1 (MQ=60)
                                  CGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATA                                                             >  SRR4302534.2654877/1‑94 (MQ=60)
                                   GGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAG                                                            <  SRR4302534.2027127/94‑1 (MQ=60)
                                   GGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAG                                                            >  SRR4302534.4653753/1‑94 (MQ=60)
                                      GGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGG                                                         <  SRR4302534.640319/94‑1 (MQ=60)
                                       GTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGT                                                        >  SRR4302534.4909301/1‑94 (MQ=60)
                                        TTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTT                                                       >  SRR4302534.4646136/1‑94 (MQ=60)
                                        TTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTT                                                       <  SRR4302534.6566673/94‑1 (MQ=60)
                                        TTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTT                                                       <  SRR4302534.6105345/94‑1 (MQ=60)
                                         tcgTTGCATGCGCAGCCCAAAGGCCACATTTTCCAACACGGGCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTT                                                      <  SRR4302534.1489835/91‑1 (MQ=60)
                                         TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTT                                                      <  SRR4302534.1445600/94‑1 (MQ=60)
                                         TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTT                                                      <  SRR4302534.5281930/94‑1 (MQ=60)
                                          TTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTT                                                     <  SRR4302534.3346143/94‑1 (MQ=60)
                                          TTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTT                                                     <  SRR4302534.5402565/94‑1 (MQ=60)
                                            TTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCC                                                   <  SRR4302534.5193001/94‑1 (MQ=60)
                                            TTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCC                                                   <  SRR4302534.1129768/94‑1 (MQ=60)
                                              GCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGAGCGTAGCTTTGGCAAACAGTGTTCACATAGCGGTTTTCCGC                                                 >  SRR4302534.1769812/1‑94 (MQ=60)
                                                ATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCG                                               <  SRR4302534.1605191/94‑1 (MQ=60)
                                                ATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCG                                               <  SRR4302534.1503904/94‑1 (MQ=60)
                                                 TGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGG                                              <  SRR4302534.6029365/94‑1 (MQ=60)
                                                  GCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGA                                             <  SRR4302534.3448579/94‑1 (MQ=60)
                                                   CGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAA                                            <  SRR4302534.783874/94‑1 (MQ=60)
                                                   CGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAA                                            <  SRR4302534.2504322/94‑1 (MQ=60)
                                                     CAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG                                          >  SRR4302534.5006093/1‑94 (MQ=60)
                                                     CAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG                                          >  SRR4302534.5264433/1‑94 (MQ=60)
                                                     CAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG                                          <  SRR4302534.599126/94‑1 (MQ=60)
                                                     CAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG                                          <  SRR4302534.1115922/94‑1 (MQ=60)
                                                     CAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG                                          <  SRR4302534.1309919/94‑1 (MQ=60)
                                                     CAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG                                          <  SRR4302534.3425589/94‑1 (MQ=60)
                                                     CAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG                                          <  SRR4302534.3671553/94‑1 (MQ=60)
                                                       ACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTG                                        <  SRR4302534.5006548/94‑1 (MQ=60)
                                                       ACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTG                                        <  SRR4302534.226011/94‑1 (MQ=60)
                                                        CCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGG                                       >  SRR4302534.1644943/1‑94 (MQ=60)
                                                        CCCAAAGGCCACATTTTCGAACACGGTCATGGGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGG                                       >  SRR4302534.2904762/1‑94 (MQ=60)
                                                         CCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG                                      <  SRR4302534.3038388/94‑1 (MQ=60)
                                                          CAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGT                                     <  SRR4302534.6375401/94‑1 (MQ=60)
                                                          CAAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGG                                     >  SRR4302534.2790136/1‑94 (MQ=60)
                                                           AAAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTG                                    <  SRR4302534.4306485/94‑1 (MQ=60)
                                                            AAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGA                                   >  SRR4302534.3352508/1‑94 (MQ=60)
                                                            AAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGA                                   >  SRR4302534.5083587/1‑94 (MQ=60)
                                                            AAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGA                                   <  SRR4302534.2554950/94‑1 (MQ=60)
                                                            AAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGA                                   >  SRR4302534.3199700/1‑94 (MQ=60)
                                                            AAGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGGTTTCCGCCGGAACGTGGGTGA                                   >  SRR4302534.2479008/1‑94 (MQ=60)
                                                             AGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGAT                                  <  SRR4302534.156033/94‑1 (MQ=60)
                                                              GGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATG                                 <  SRR4302534.1666148/94‑1 (MQ=60)
                                                               GCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGGACGTGGGTGATGT                                >  SRR4302534.3847601/1‑94 (MQ=60)
                                                               GCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGT                                >  SRR4302534.4120006/1‑94 (MQ=60)
                                                                CCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTC                               >  SRR4302534.183205/1‑94 (MQ=60)
                                                                CCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTC                               <  SRR4302534.2794760/94‑1 (MQ=60)
                                                                 CACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCC                              >  SRR4302534.3418805/1‑94 (MQ=60)
                                                                 CACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCC                              >  SRR4302534.3140356/1‑94 (MQ=60)
                                                                 CACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCC                              <  SRR4302534.3568585/94‑1 (MQ=60)
                                                                 CACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCC                              >  SRR4302534.2602295/1‑94 (MQ=60)
                                                                 CACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCC                              >  SRR4302534.5113083/1‑94 (MQ=60)
                                                                 CACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCC                              >  SRR4302534.343520/1‑94 (MQ=60)
                                                                   CATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTC                            >  SRR4302534.304956/1‑94 (MQ=60)
                                                                   CATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTC                            <  SRR4302534.6311621/94‑1 (MQ=60)
                                                                   CATTGTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTC                            <  SRR4302534.5249843/94‑1 (MQ=60)
                                                                    ATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCG                           >  SRR4302534.6215425/1‑94 (MQ=60)
                                                                    ATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCG                           <  SRR4302534.2602099/94‑1 (MQ=60)
                                                                    ATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCG                           <  SRR4302534.4026524/94‑1 (MQ=60)
                                                                      TTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTT                         <  SRR4302534.3996106/94‑1 (MQ=60)
                                                                         CGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATC                      <  SRR4302534.3619482/94‑1 (MQ=60)
                                                                         CGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATC                      >  SRR4302534.5492908/1‑94 (MQ=60)
                                                                          GAACGCAGTCATTTGGGGGGCAAGTGCGTAGCTTTGGAAAATATTGCTCACATAGCGGTTTTCCGCCGGAACGTGGGGGATGTCGTCGTTgtgc                     >  SRR4302534.2405426/1‑90 (MQ=43)
                                                                          GAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCC                     >  SRR4302534.2925078/1‑94 (MQ=60)
                                                                          GAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCC                     >  SRR4302534.1037906/1‑94 (MQ=60)
                                                                           AACACGGTCATGTGGGGGGACAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGGTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCA                    >  SRR4302534.6445249/1‑94 (MQ=60)
                                                                           AACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCA                    >  SRR4302534.643694/1‑94 (MQ=60)
                                                                           AACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCA                    <  SRR4302534.5055229/94‑1 (MQ=60)
                                                                            ACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAG                   <  SRR4302534.3975746/94‑1 (MQ=60)
                                                                             CACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGC                  >  SRR4302534.2600463/1‑94 (MQ=60)
                                                                              ACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCA                 <  SRR4302534.1418408/94‑1 (MQ=60)
                                                                               CGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCAT                <  SRR4302534.3253712/94‑1 (MQ=60)
                                                                                  TCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGAT             <  SRR4302534.5626692/94‑1 (MQ=60)
                                                                                  TCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGAT             >  SRR4302534.1783411/1‑94 (MQ=60)
                                                                                  TCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGAT             <  SRR4302534.4310498/94‑1 (MQ=60)
                                                                                   CATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATG            <  SRR4302534.4676707/94‑1 (MQ=60)
                                                                                   CATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATG            >  SRR4302534.1565449/1‑94 (MQ=60)
                                                                                   CATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATG            >  SRR4302534.2566925/1‑94 (MQ=60)
                                                                                    ATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGC           <  SRR4302534.1798067/94‑1 (MQ=60)
                                                                                    ATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGC           <  SRR4302534.6347487/94‑1 (MQ=60)
                                                                                     TGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCG          >  SRR4302534.4378406/1‑94 (MQ=60)
                                                                                      GTGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGT         <  SRR4302534.5057809/94‑1 (MQ=60)
                                                                                      GTAGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGT         >  SRR4302534.6546287/1‑94 (MQ=60)
                                                                                       TGGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTC        <  SRR4302534.3897273/94‑1 (MQ=60)
                                                                                        GGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCC       <  SRR4302534.3445540/94‑1 (MQ=60)
                                                                                        GGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCC       >  SRR4302534.5363103/1‑94 (MQ=60)
                                                                                        GGGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCATCC       <  SRR4302534.6403072/94‑1 (MQ=60)
                                                                                         GGGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCG      <  SRR4302534.4427458/94‑1 (MQ=60)
                                                                                          GGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGG     <  SRR4302534.6370997/94‑1 (MQ=60)
                                                                                          GGGGAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGG     <  SRR4302534.1814025/94‑1 (MQ=60)
                                                                                             GAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAAT  <  SRR4302534.3500175/94‑1 (MQ=60)
                                                                                             GAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAAT  >  SRR4302534.2097267/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAAT  >  NC_000913/1184423‑1184609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑