Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,220,688 C→T T87I (ACC→ATC)  b1170 → putative part of putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,220,6880CT99.1% 867.2 / ‑3.8 231T87I (ACC→ATC) b1170putative part of putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (99/129);  total (100/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

Only 100 of 239 total aligned reads displayed.

TAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTG  >  NC_000913/1220598‑1220777
                                                                                          |                                                                                         
tAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCt                                                                                         <  2:2652293/93‑1 (MQ=255)
 aaCTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGAtctc                                                                                        >  1:443468/1‑93 (MQ=255)
 aaCTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGAtctc                                                                                        <  2:4299818/93‑1 (MQ=255)
     gAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCAt                                                                                    <  1:100221/93‑1 (MQ=255)
      aaCAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATc                                                                                   <  2:6178536/93‑1 (MQ=255)
       aCAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCg                                                                                  >  1:3938672/1‑93 (MQ=255)
       aCAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCg                                                                                  >  2:3767499/1‑93 (MQ=255)
       aCAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCg                                                                                  >  1:1859915/1‑93 (MQ=255)
       aCAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGGTGAGAACGATCTCGCAGCg                                                                                  >  1:421974/1‑93 (MQ=255)
        cAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGc                                                                                 <  2:5648101/93‑1 (MQ=255)
        cAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGc                                                                                 <  2:5452164/93‑1 (MQ=255)
         aGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCt                                                                                >  1:72434/1‑93 (MQ=255)
           gAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTAc                                                                              <  1:4566558/93‑1 (MQ=255)
             gCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGt                                                                            <  1:204272/93‑1 (MQ=255)
             gCGTTCAAGCCGACTTTGGCTACGTC‑AATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGtc                                                                           <  2:803446/93‑1 (MQ=255)
              cGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGtc                                                                           <  1:6200068/93‑1 (MQ=255)
               gTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGtct                                                                          <  2:727434/93‑1 (MQ=255)
                ttCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGtctc                                                                         <  2:1479118/93‑1 (MQ=255)
                  cAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGc                                                                       >  2:531193/1‑93 (MQ=255)
                  cAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTCAGAACGATCTCCCAACGCTACGTCTCGc                                                                       >  2:4302423/1‑93 (MQ=255)
                  cAAGCCGACTTTGGCTAAGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGc                                                                       <  2:3840855/93‑1 (MQ=255)
                    aGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAg                                                                     >  1:149936/1‑93 (MQ=255)
                     gCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGt                                                                    <  1:4241509/93‑1 (MQ=255)
                      ccGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGtt                                                                   <  2:1770220/93‑1 (MQ=255)
                       cGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTa                                                                  >  2:4692204/1‑93 (MQ=255)
                        gACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAg                                                                 <  1:4052911/93‑1 (MQ=255)
                        gACTTTGGCTACGTCAAATTGGCAGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAg                                                                 >  1:2876504/1‑93 (MQ=255)
                         aCTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAgg                                                                <  1:5035900/93‑1 (MQ=255)
                          cTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAggg                                                               >  2:1759333/1‑93 (MQ=255)
                          cTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAggg                                                               <  1:5825485/93‑1 (MQ=255)
                          cTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAggg                                                               <  1:4863891/93‑1 (MQ=255)
                           tttGGCTACGTTAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAgggg                                                              <  1:1702313/93‑1 (MQ=255)
                            ttGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAggggg                                                             <  2:5174901/93‑1 (MQ=255)
                             tGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGc                                                            <  2:2603550/93‑1 (MQ=255)
                              ggCTACGTCAAATTGGCGGGCATAATGACTGGCGTGACAGCAACGGACAGTTGAGACCGATCTCCCATCGCTACGTGTCGCCGTTAGGGGGCg                                                           >  1:3148443/1‑93 (MQ=255)
                               gCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGa                                                          >  2:6372961/1‑93 (MQ=255)
                               gCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGa                                                          >  2:4883538/1‑93 (MQ=255)
                               gCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGa                                                          >  2:3732390/1‑93 (MQ=255)
                                cTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGAt                                                         >  1:613039/1‑93 (MQ=255)
                                cTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGAt                                                         >  2:2745963/1‑93 (MQ=255)
                                 tACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATc                                                        >  2:1931561/1‑93 (MQ=255)
                                  aCGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCt                                                       >  1:6012019/1‑93 (MQ=255)
                                  aCGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCt                                                       <  2:2984819/93‑1 (MQ=255)
                                     tCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGtt                                                    >  2:1052976/1‑93 (MQ=255)
                                       aaaTTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTaa                                                  <  2:2145032/93‑1 (MQ=255)
                                       aaaTTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTaa                                                  <  1:799216/93‑1 (MQ=255)
                                       aaaTTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTaa                                                  >  1:4631807/1‑93 (MQ=255)
                                        aaTTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAAc                                                 >  2:1368841/1‑93 (MQ=255)
                                        aaTTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAAc                                                 <  1:2343507/93‑1 (MQ=255)
                                            ggCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCggt                                             <  1:2351705/93‑1 (MQ=255)
                                             gCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCggtg                                            <  2:4902387/93‑1 (MQ=255)
                                              cGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCggtgg                                           <  1:4085709/93‑1 (MQ=255)
                                                ggCATAATGGCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGtt                                         <  2:2014705/93‑1 (MQ=255)
                                                ggCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGtt                                         <  2:4667032/93‑1 (MQ=255)
                                                ggCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGtt                                         <  1:3451809/93‑1 (MQ=255)
                                                ggCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGtt                                         <  2:5980773/93‑1 (MQ=255)
                                                   aTAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTa                                      >  1:5077478/1‑93 (MQ=255)
                                                   aTAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTa                                      >  2:898864/1‑93 (MQ=255)
                                                    tAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTAc                                     >  2:1277212/1‑93 (MQ=255)
                                                    tAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTAc                                     >  1:3878262/1‑93 (MQ=255)
                                                     aaTGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTAcc                                    >  1:5940666/1‑93 (MQ=255)
                                                     aaTGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTAcc                                    >  1:5451652/1‑93 (MQ=255)
                                                      aTGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCg                                   >  1:6065120/1‑93 (MQ=255)
                                                      aTGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCg                                   <  2:4632872/93‑1 (MQ=255)
                                                       tGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGa                                  >  1:5741245/1‑93 (MQ=255)
                                                        gCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGAt                                 <  1:1208053/93‑1 (MQ=255)
                                                        gCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGAt                                 <  2:482356/93‑1 (MQ=255)
                                                        gCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGAt                                 <  1:548364/93‑1 (MQ=255)
                                                         ccTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATa                                <  2:336285/93‑1 (MQ=255)
                                                          cTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAg                               >  1:3739536/1‑93 (MQ=255)
                                                           tGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGt                              >  2:852162/1‑93 (MQ=255)
                                                           tGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGt                              <  2:1649999/93‑1 (MQ=255)
                                                           tGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGt                              <  2:5559096/93‑1 (MQ=255)
                                                             gCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTTCCGATAGTGa                            >  1:109273/1‑93 (MQ=255)
                                                              cGTAACAGCAACGCACAGTGGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGAc                           <  2:171072/93‑1 (MQ=255)
                                                               gTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACa                          >  1:4519446/1‑93 (MQ=255)
                                                               gTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACa                          >  1:3386043/1‑93 (MQ=255)
                                                                tGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAg                         >  2:3317633/1‑93 (MQ=255)
                                                                  aCAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGtt                       <  1:4601096/93‑1 (MQ=255)
                                                                  aCAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGtt                       <  2:2937947/93‑1 (MQ=255)
                                                                   cAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTg                      >  1:2123262/1‑93 (MQ=255)
                                                                      cAACGGACAGTTGAGAACGACCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCg                   >  2:4932236/1‑93 (MQ=255)
                                                                       aaCGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGt                  >  1:5369375/1‑93 (MQ=255)
                                                                        aCGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGtt                 <  1:5141694/93‑1 (MQ=255)
                                                                        aCGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGtt                 <  1:5675215/93‑1 (MQ=255)
                                                                        aCGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGtt                 >  2:283801/1‑93 (MQ=255)
                                                                          ggACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTg               >  1:2591308/1‑93 (MQ=255)
                                                                           gACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTgg              >  1:5208749/1‑93 (MQ=255)
                                                                             cAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGa            >  2:2324846/1‑93 (MQ=255)
                                                                                ttGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTg         >  1:3549066/1‑93 (MQ=255)
                                                                                 tGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGa        <  2:5258506/93‑1 (MQ=255)
                                                                                 tGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGa        <  1:2982813/93‑1 (MQ=255)
                                                                                  gagaACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGAt       <  2:5841282/93‑1 (MQ=255)
                                                                                  gagaACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGAt       <  2:2450143/93‑1 (MQ=255)
                                                                                   agaACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATg      >  1:3190754/1‑93 (MQ=255)
                                                                                   agaACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATg      >  2:2406347/1‑93 (MQ=255)
                                                                                    gaACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATgg     >  2:6093279/1‑93 (MQ=255)
                                                                                     aaCGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGc    >  2:4845347/1‑93 (MQ=255)
                                                                                      aCGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCt   <  1:2799411/93‑1 (MQ=255)
                                                                                       cGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTg  <  2:3662596/93‑1 (MQ=255)
                                                                                          |                                                                                         
TAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTG  >  NC_000913/1220598‑1220777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTA  >  NC_000913/1220596‑1220781
                                                                                            |                                                                                             
TGTAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATC                                                                                              <  SRR4302534.37270/94‑1 (MQ=60)
TGTAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATC                                                                                              >  SRR4302534.4570315/1‑94 (MQ=60)
TGTAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATC                                                                                              <  SRR4302534.2781303/94‑1 (MQ=60)
 GTAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCT                                                                                             <  SRR4302534.4966536/94‑1 (MQ=60)
  TAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTC                                                                                            >  SRR4302534.454724/1‑94 (MQ=60)
  TAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTC                                                                                            >  SRR4302534.476819/1‑94 (MQ=60)
    ACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGC                                                                                          <  SRR4302534.4349558/94‑1 (MQ=60)
     CTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCTTGACAGCAACGGACAGTTGAGAAAGATCTCGCA                                                                                         >  SRR4302534.735174/1‑94 (MQ=60)
       GAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATC                                                                                       <  SRR4302534.102901/94‑1 (MQ=60)
       GAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATC                                                                                       <  SRR4302534.2810649/94‑1 (MQ=60)
        AACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCG                                                                                      >  SRR4302534.4044163/1‑94 (MQ=60)
        AACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCG                                                                                      >  SRR4302534.1908321/1‑94 (MQ=60)
        AACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGGTGAGAACGATCTCGCAGCG                                                                                      >  SRR4302534.432762/1‑94 (MQ=60)
         ACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGC                                                                                     >  SRR4302534.3573475/1‑94 (MQ=60)
          CAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCT                                                                                    >  SRR4302534.74413/1‑94 (MQ=60)
          CAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCT                                                                                    <  SRR4302534.3322766/94‑1 (MQ=60)
           AGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTA                                                                                   >  SRR4302534.4192293/1‑94 (MQ=60)
           AGGAGCGTGCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTA                                                                                   >  SRR4302534.671577/1‑94 (MQ=60)
            GGAGCGTTCAAGCCGACTTTGGCTAAGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTAC                                                                                  <  SRR4302534.4457844/94‑1 (MQ=60)
             GAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACG                                                                                 <  SRR4302534.4690354/94‑1 (MQ=60)
               GCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTC                                                                               <  SRR4302534.209634/94‑1 (MQ=60)
                CGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCT                                                                              >  SRR4302534.4571936/1‑94 (MQ=60)
                CGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCT                                                                              <  SRR4302534.6368717/94‑1 (MQ=60)
                CGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCT                                                                              <  SRR4302534.5224452/94‑1 (MQ=60)
                 GTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTC                                                                             <  SRR4302534.2409270/94‑1 (MQ=60)
                  TTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCG                                                                            <  SRR4302534.1118959/94‑1 (MQ=60)
                  TTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCG                                                                            <  SRR4302534.5266364/94‑1 (MQ=60)
                  GTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCG                                                                            <  SRR4302534.1349417/94‑1 (MQ=60)
                   TCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGC                                                                           <  SRR4302534.2393629/94‑1 (MQ=60)
                     AAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAG                                                                         >  SRR4302534.153846/1‑94 (MQ=60)
                     AAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAG                                                                         >  SRR4302534.4224159/1‑94 (MQ=60)
                      AGCCGACTTTGGCTACGTCAAATTGGCGGGCCTAATGCCTTGCGTGACAGCAACGGACAGGTGAGAACGATCTCGCATCGCTgcggctcggcgg                                                                        >  SRR4302534.216323/1‑82 (MQ=60)
                      AGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGT                                                                        >  SRR4302534.2807881/1‑94 (MQ=60)
                       GCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTT                                                                       <  SRR4302534.4355879/94‑1 (MQ=60)
                       GCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTT                                                                       <  SRR4302534.3642048/94‑1 (MQ=60)
                        CCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTA                                                                      <  SRR4302534.4800682/94‑1 (MQ=60)
                        CCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTA                                                                      <  SRR4302534.6005934/94‑1 (MQ=60)
                         CGACTTTGGCTACGTCAAATTGGCAGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAG                                                                     >  SRR4302534.2952016/1‑94 (MQ=60)
                          GACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGG                                                                    >  SRR4302534.4596923/1‑94 (MQ=60)
                          GACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGG                                                                    >  SRR4302534.5630463/1‑94 (MQ=60)
                          GACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGG                                                                    <  SRR4302534.4161621/94‑1 (MQ=60)
                           ACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGG                                                                   <  SRR4302534.5171916/94‑1 (MQ=60)
                           ACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGG                                                                   <  SRR4302534.5849791/94‑1 (MQ=60)
                            CTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGG                                                                  <  SRR4302534.4995837/94‑1 (MQ=60)
                            CTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGG                                                                  <  SRR4302534.5983609/94‑1 (MQ=60)
                             TTTGGCTACGTTAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGG                                                                 <  SRR4302534.1746540/94‑1 (MQ=60)
                             TTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGG                                                                 <  SRR4302534.6177434/94‑1 (MQ=60)
                               TGGCTACGTCAAATTGGCGGGCATAATGACTGGCGTGACAGCAACGGACAGTTGAGACCGATCTCCCATCGCTACGTGTCGCCGTTAGGGGGCG                                                               >  SRR4302534.3231270/1‑94 (MQ=60)
                                GGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCCGTTAGGGGGCGA                                                              >  SRR4302534.3494909/1‑94 (MQ=60)
                                 GCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGAT                                                             >  SRR4302534.627935/1‑94 (MQ=60)
                                 GCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGAT                                                             <  SRR4302534.6041686/94‑1 (MQ=60)
                                  CTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATC                                                            >  SRR4302534.899593/1‑94 (MQ=60)
                                   TACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCT                                                           >  SRR4302534.880124/1‑94 (MQ=60)
                                   TACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCT                                                           >  SRR4302534.6175376/1‑94 (MQ=60)
                                     CGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGCTCTGT                                                         >  SRR4302534.792646/1‑94 (MQ=60)
                                     CGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGT                                                         <  SRR4302534.1218504/94‑1 (MQ=60)
                                      GTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTT                                                        >  SRR4302534.5192922/1‑94 (MQ=60)
                                      GTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTT                                                        <  SRR4302534.3470959/94‑1 (MQ=60)
                                        CAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAA                                                      >  SRR4302534.4757492/1‑94 (MQ=60)
                                         AAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAAC                                                     <  SRR4302534.819284/94‑1 (MQ=60)
                                         AAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAAC                                                     <  SRR4302534.5297816/94‑1 (MQ=60)
                                         AAATTGGCGGGAATAATGCCTGGCGTGACAGCAAAAGACAGTTGAGAACGATCTCGCATAGGTACGTCTCGCAGTTAGGGGGAGGTCTGTTAAC                                                     >  SRR4302534.6016386/1‑94 (MQ=60)
                                          AATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACC                                                    >  SRR4302534.6185564/1‑94 (MQ=60)
                                          AATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACC                                                    <  SRR4302534.2405702/94‑1 (MQ=60)
                                           ATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCG                                                   <  SRR4302534.4118220/94‑1 (MQ=60)
                                             TGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGT                                                 <  SRR4302534.400576/94‑1 (MQ=60)
                                             TGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGT                                                 <  SRR4302534.4723626/94‑1 (MQ=60)
                                              GGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTG                                                <  SRR4302534.208860/94‑1 (MQ=60)
                                              GGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTG                                                <  SRR4302534.2414123/94‑1 (MQ=60)
                                                CGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGT                                              <  SRR4302534.4195419/94‑1 (MQ=60)
                                                 GGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTT                                             >  SRR4302534.5440465/1‑94 (MQ=60)
                                                  GGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTT                                            <  SRR4302534.3544605/94‑1 (MQ=60)
                                                  GGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGCCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTT                                            <  SRR4302534.5815756/94‑1 (MQ=60)
                                                    CATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTA                                          >  SRR4302534.5214545/1‑94 (MQ=60)
                                                     ATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTAC                                         >  SRR4302534.3982398/1‑94 (MQ=60)
                                                     ATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTAC                                         >  SRR4302534.6323094/1‑94 (MQ=60)
                                                      TAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACC                                        >  SRR4302534.6101967/1‑94 (MQ=60)
                                                      TAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACC                                        >  SRR4302534.5598545/1‑94 (MQ=60)
                                                       AATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCG                                       >  SRR4302534.6230023/1‑94 (MQ=60)
                                                       AATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCG                                       >  SRR4302534.6169519/1‑94 (MQ=60)
                                                        ATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGA                                      <  SRR4302534.3019799/94‑1 (MQ=60)
                                                        ATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGA                                      >  SRR4302534.5896855/1‑94 (MQ=60)
                                                          GCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATA                                    <  SRR4302534.561894/94‑1 (MQ=60)
                                                          GCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATA                                    <  SRR4302534.1239844/94‑1 (MQ=60)
                                                          GCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATA                                    <  SRR4302534.6296579/94‑1 (MQ=60)
                                                          GCCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATA                                    <  SRR4302534.5931264/94‑1 (MQ=60)
                                                           CCTGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAG                                   >  SRR4302534.3840012/1‑94 (MQ=60)
                                                             TGGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTG                                 <  SRR4302534.4714262/94‑1 (MQ=60)
                                                              GGCGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTTCCGATAGTGA                                >  SRR4302534.112190/1‑94 (MQ=60)
                                                                CGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACA                              >  SRR4302534.3476850/1‑94 (MQ=60)
                                                                CGTGACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACA                              >  SRR4302534.4641873/1‑94 (MQ=60)
                                                                   GACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTT                           <  SRR4302534.4318828/94‑1 (MQ=60)
                                                                   GACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTT                           <  SRR4302534.5644546/94‑1 (MQ=60)
                                                                    ACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTG                          <  SRR4302534.125329/94‑1 (MQ=60)
                                                                    ACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTG                          <  SRR4302534.4725965/94‑1 (MQ=60)
                                                                    ACAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTG                          >  SRR4302534.2179175/1‑94 (MQ=60)
                                                                     CAGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGG                         <  SRR4302534.1606793/94‑1 (MQ=60)
                                                                      AGCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGC                        <  SRR4302534.512523/94‑1 (MQ=60)
                                                                       GCAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCG                       <  SRR4302534.2949420/94‑1 (MQ=60)
                                                                        CAACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGT                      >  SRR4302534.5514071/1‑94 (MQ=60)
                                                                         AACGGACAGTTGAGAACGAACTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTT                     <  SRR4302534.3260046/94‑1 (MQ=60)
                                                                          ACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTT                    <  SRR4302534.5828861/94‑1 (MQ=60)
                                                                          ACGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTT                    <  SRR4302534.5280452/94‑1 (MQ=60)
                                                                           CGGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTG                   >  SRR4302534.2659905/1‑94 (MQ=60)
                                                                            GGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGG                  <  SRR4302534.2096856/94‑1 (MQ=60)
                                                                            GGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGG                  <  SRR4302534.232171/94‑1 (MQ=60)
                                                                            GGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGG                  >  SRR4302534.1804831/1‑94 (MQ=60)
                                                                            GGACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGG                  >  SRR4302534.5349317/1‑94 (MQ=60)
                                                                             GACAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGG                 <  SRR4302534.3674921/94‑1 (MQ=60)
                                                                               CAGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAG               >  SRR4302534.3141776/1‑94 (MQ=60)
                                                                                AGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACCGTTGGCGTTTGGGAGG              >  SRR4302534.2645650/1‑94 (MQ=60)
                                                                                AGTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGT              <  SRR4302534.5575413/94‑1 (MQ=60)
                                                                                 GTTGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTG             >  SRR4302534.3644503/1‑94 (MQ=60)
                                                                                   TGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGAT           >  SRR4302534.2043871/1‑94 (MQ=60)
                                                                                   TGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGAT           <  SRR4302534.3060848/94‑1 (MQ=60)
                                                                                   TGAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGAT           >  SRR4302534.3999768/1‑94 (MQ=60)
                                                                                    GAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATG          <  SRR4302534.3042263/94‑1 (MQ=60)
                                                                                    GAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATG          <  SRR4302534.4371406/94‑1 (MQ=60)
                                                                                    GAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATG          >  SRR4302534.3275232/1‑94 (MQ=60)
                                                                                    GAGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATG          <  SRR4302534.3889740/94‑1 (MQ=60)
                                                                                     AGAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGG         <  SRR4302534.5380797/94‑1 (MQ=60)
                                                                                      GAACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGC        >  SRR4302534.4679200/1‑94 (MQ=60)
                                                                                        ACGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTG      <  SRR4302534.2873037/94‑1 (MQ=60)
                                                                                         CGATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGG     <  SRR4302534.4734370/94‑1 (MQ=60)
                                                                                          GATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGT    >  SRR4302534.1085475/1‑94 (MQ=60)
                                                                                          GATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGT    >  SRR4302534.4434640/1‑94 (MQ=60)
                                                                                          GATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGT    >  SRR4302534.5775977/1‑94 (MQ=60)
                                                                                          GATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGT    >  SRR4302534.4642444/1‑94 (MQ=60)
                                                                                           ATCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTT   <  SRR4302534.5759412/94‑1 (MQ=60)
                                                                                            TCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTA  <  SRR4302534.3094142/94‑1 (MQ=60)
                                                                                            TCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTA  <  SRR4302534.3951753/94‑1 (MQ=60)
                                                                                            |                                                                                             
TGTAACTGAACAGGAGCGTTCAAGCCGACTTTGGCTACGTCAAATTGGCGGGCATAATGCCTGGCGTGACAGCAACGGACAGTTGAGAACGACCTCGCATCGCTACGTCTCGCAGTTAGGGGGCGATCTGTTAACCGGTGGTTTTACCGATAGTGACAGTTGGCGTTTGGGAGTGATGGCTGGTTA  >  NC_000913/1220596‑1220781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑