Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,330,060	A→G	D330G (GAC→GGC)	topA →	DNA topoisomerase type I omega protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,330,060	0	A	G	99.7%	1013.9 / ‑4.7	293	D330G (GAC→GGC)	topA	DNA topoisomerase type I omega protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (141/151); total (142/151)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

Only 100 of 299 total aligned reads displayed.

GCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAA  >  NC_000913/1329970‑1330149

 cGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGgcgc                                                                                        <  1:3738264/93‑1 (MQ=255)

  gTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGcgcg                                                                                       >  1:1535337/1‑93 (MQ=255)

  gTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGcgcg                                                                                       >  1:4397544/1‑93 (MQ=255)

   tGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCgg                                                                                      <  2:71533/93‑1 (MQ=255)

   tGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCgg                                                                                      <  1:6012215/93‑1 (MQ=255)

    gAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGt                                                                                     >  2:149535/1‑93 (MQ=255)

    gAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGt                                                                                     >  2:4257819/1‑93 (MQ=255)

       aaaaaCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTaaa                                                                                  <  2:1378694/93‑1 (MQ=255)

        aaaaCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAat                                                                                 <  2:5392754/93‑1 (MQ=255)

        aaaaCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAat                                                                                 >  2:4650883/1‑93 (MQ=255)

        aaaaCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAat                                                                                 <  1:3517125/93‑1 (MQ=255)

          aaCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAatat                                                                               >  2:2305415/1‑93 (MQ=255)

           aCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATg                                                                              >  1:6177627/1‑93 (MQ=255)

           aCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATg                                                                              >  2:1889722/1‑93 (MQ=255)

           aCCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATg                                                                              >  1:4016776/1‑93 (MQ=255)

            ccATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATgg                                                                             >  1:4204894/1‑93 (MQ=255)

              atgatgatgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAGTATGGtt                                                                           >  2:6279489/1‑93 (MQ=255)

              atgatgatgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGtt                                                                           <  1:891217/93‑1 (MQ=255)

              atgatgatgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGtt                                                                           >  1:4754283/1‑93 (MQ=255)

              atgatgatgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGtt                                                                           >  1:695895/1‑93 (MQ=255)

                gatgatgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTcg                                                                         >  2:5490138/1‑93 (MQ=255)

                   gatgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCgg                                                                      >  1:525207/1‑93 (MQ=255)

                    atgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGt                                                                     >  2:2644976/1‑93 (MQ=255)

                    atgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGt                                                                     <  1:1387497/93‑1 (MQ=255)

                    atgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGt                                                                     <  2:4009814/93‑1 (MQ=255)

                     tgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGtt                                                                    <  1:5839811/93‑1 (MQ=255)

                     tgGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGtt                                                                    <  2:5945909/93‑1 (MQ=255)

                       gcgcAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTtat                                                                  <  1:2006167/93‑1 (MQ=255)

                        cgcAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTtata                                                                 <  1:3034680/93‑1 (MQ=255)

                         gcAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTatat                                                                >  2:3576106/1‑93 (MQ=255)

                           aGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCa                                                              >  2:75906/1‑93 (MQ=255)

                            gCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAg                                                             >  2:4292914/1‑93 (MQ=255)

                             cGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGc                                                            <  2:1254917/93‑1 (MQ=255)

                              gTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCg                                                           <  1:5622543/93‑1 (MQ=255)

                                ttGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGAt                                                         >  1:2221428/1‑93 (MQ=255)

                                  gTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATCTCAGCGATaa                                                       >  2:960675/1‑93 (MQ=255)

                                  gTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATaa                                                       >  1:2404692/1‑93 (MQ=255)

                                  gTATAAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATaa                                                       >  1:1142210/1‑93 (MQ=255)

                                   tATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAt                                                      <  2:2481380/93‑1 (MQ=255)

                                   tATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAt                                                      <  2:5258733/93‑1 (MQ=255)

                                    aTGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAtt                                                     >  2:226888/1‑93 (MQ=255)

                                     tGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAttt                                                    <  1:5491275/93‑1 (MQ=255)

                                      gAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAtttt                                                   >  2:4070753/1‑93 (MQ=255)

                                      gAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAtttt                                                   <  2:2975579/93‑1 (MQ=255)

                                       aaGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTg                                                  >  1:4519543/1‑93 (MQ=255)

                                        aGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTgg                                                 >  1:4059305/1‑93 (MQ=255)

                                         gCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTGAATATGGTTCGCGGTTATATCAGCGATAATTTTGGt                                                >  2:1693502/1‑93 (MQ=255)

                                          cAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTa                                               <  1:2104289/93‑1 (MQ=255)

                                          cAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTa                                               <  1:2993062/93‑1 (MQ=255)

                                          cAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTa                                               <  2:2064284/93‑1 (MQ=255)

                                           aGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTaa                                              <  2:3007070/93‑1 (MQ=255)

                                           aGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTaa                                              <  1:3216075/93‑1 (MQ=255)

                                           aGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTaa                                              <  1:3626540/93‑1 (MQ=255)

                                           aGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTaa                                              >  1:1882838/1‑93 (MQ=255)

                                            ggCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAg                                             <  1:4810732/93‑1 (MQ=255)

                                            ggCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAg                                             <  2:1068977/93‑1 (MQ=255)

                                              cTATATCACTTACATGCGTACCGACTCCACTAACCTGCGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGaa                                           >  2:2761882/1‑93 (MQ=255)

                                              cTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGaa                                           >  2:3453855/1‑93 (MQ=255)

                                               tataTCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGaaa                                          >  1:5377282/1‑93 (MQ=255)

                                               tataTCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGaaa                                          >  2:4571185/1‑93 (MQ=255)

                                                atatCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAat                                         >  1:2736150/1‑93 (MQ=255)

                                                atatCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAat                                         <  2:6201611/93‑1 (MQ=255)

                                                 tatCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAata                                        >  1:2271932/1‑93 (MQ=255)

                                                  atCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAatat                                       >  2:517767/1‑93 (MQ=255)

                                                  atCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAatat                                       >  2:3528848/1‑93 (MQ=255)

                                                    cACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCt                                     <  2:5713681/93‑1 (MQ=255)

                                                     aCTTACATGCGTCCCGACTCCACTAACCCGAGTCAGGGCGCGGTAAATATGGTTCGCGGCTATATCAGCGATAATTTTGGTAAGAAATATCTg                                    >  1:934351/1‑93 (MQ=255)

                                                      cTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGc                                   >  1:1241277/1‑93 (MQ=255)

                                                      cTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGc                                   <  2:4805160/93‑1 (MQ=255)

                                                        tACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCg                                 <  2:4152896/93‑1 (MQ=255)

                                                           aTGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGaa                              >  2:4982566/1‑93 (MQ=255)

                                                            tGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGaaa                             <  1:2548787/93‑1 (MQ=255)

                                                            tGCGTACCGACTCCACCAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGaaa                             <  2:5971874/93‑1 (MQ=255)

                                                              cGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGt                           >  2:1999311/1‑93 (MQ=255)

                                                               gTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTc                          >  1:1033238/1‑93 (MQ=255)

                                                               gTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTc                          <  1:2755755/93‑1 (MQ=255)

                                                                tACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTcc                         >  2:4607713/1‑93 (MQ=255)

                                                                  ccGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGa                       >  1:631354/1‑93 (MQ=255)

                                                                  ccGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGa                       <  2:4962751/93‑1 (MQ=255)

                                                                   cGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGaa                      <  2:823193/93‑1 (MQ=255)

                                                                   cGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGaa                      <  1:430508/93‑1 (MQ=255)

                                                                   cGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGaa                      <  1:4933251/93‑1 (MQ=255)

                                                                    gACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAt                     >  1:554091/1‑93 (MQ=255)

                                                                    gACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAt                     >  2:2591027/1‑93 (MQ=255)

                                                                    gACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAt                     >  2:6069784/1‑93 (MQ=255)

                                                                     aCTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATc                    <  2:1208177/93‑1 (MQ=255)

                                                                      cTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCa                   <  2:3272317/93‑1 (MQ=255)

                                                                        ccACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGt                 <  2:3694320/93‑1 (MQ=255)

                                                                         cACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTa                <  2:5839157/93‑1 (MQ=255)

                                                                             aaCCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGcc            >  2:3913843/1‑93 (MQ=255)

                                                                             aaCCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGcc            <  1:257200/93‑1 (MQ=255)

                                                                             aaCCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGcc            <  2:1139826/93‑1 (MQ=255)

                                                                             aaCCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGcc            >  2:2719507/1‑93 (MQ=255)

                                                                                 tGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCa        >  1:3368166/1‑93 (MQ=255)

                                                                                 tGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCa        >  2:5099329/1‑93 (MQ=255)

                                                                                    gTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAg     >  1:818017/1‑93 (MQ=255)

                                                                                     tCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGa    >  2:2906269/1‑93 (MQ=255)

                                                                                     tCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGa    <  1:4641886/93‑1 (MQ=255)

                                                                                      cAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGaa   <  1:64193/93‑1 (MQ=255)

                                                                                       aGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGaaa  >  1:5148902/1‑93 (MQ=255)

GCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAA  >  NC_000913/1329970‑1330149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAAC  >  NC_000913/1329967‑1330151

TTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGG                                                                                             <  SRR4302534.1119878/94‑1 (MQ=60)

TTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGG                                                                                             <  SRR4302534.2709078/94‑1 (MQ=60)

 TGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGC                                                                                            >  SRR4302534.1906150/1‑94 (MQ=60)

  GGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCG                                                                                           >  SRR4302534.6479246/1‑94 (MQ=60)

    CGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCG                                                                                         >  SRR4302534.4516190/1‑94 (MQ=60)

    CGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCG                                                                                         <  SRR4302534.3838689/94‑1 (MQ=60)

    CGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCG                                                                                         >  SRR4302534.1575602/1‑94 (MQ=60)

    CGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCG                                                                                         <  SRR4302534.1213509/94‑1 (MQ=60)

     GTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGG                                                                                        <  SRR4302534.3734824/94‑1 (MQ=60)

      TGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGT                                                                                       <  SRR4302534.6175578/94‑1 (MQ=60)

       GAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTA                                                                                      >  SRR4302534.5765631/1‑94 (MQ=60)

       GAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTA                                                                                      >  SRR4302534.5293825/1‑94 (MQ=60)

        AAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAA                                                                                     <  SRR4302534.3635823/94‑1 (MQ=60)

        AAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAA                                                                                     <  SRR4302534.2246856/94‑1 (MQ=60)

        AAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAA                                                                                     >  SRR4302534.5629343/1‑94 (MQ=60)

         AAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAA                                                                                    >  SRR4302534.895614/1‑94 (MQ=60)

           AAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATA                                                                                  <  SRR4302534.3611722/94‑1 (MQ=60)

            AAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATAT                                                                                 <  SRR4302534.5172879/94‑1 (MQ=60)

            AAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATAT                                                                                 >  SRR4302534.179062/1‑94 (MQ=60)

            AAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATAT                                                                                 <  SRR4302534.2616388/94‑1 (MQ=60)

            AAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATAT                                                                                 <  SRR4302534.719749/94‑1 (MQ=60)

             AACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATG                                                                                >  SRR4302534.6345736/1‑94 (MQ=60)

             AACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATG                                                                                >  SRR4302534.2638511/1‑94 (MQ=60)

             AACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATG                                                                                >  SRR4302534.4124449/1‑94 (MQ=60)

             AACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATG                                                                                <  SRR4302534.4795775/94‑1 (MQ=60)

              ACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGG                                                                               >  SRR4302534.2961550/1‑94 (MQ=60)

              ACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGG                                                                               >  SRR4302534.4318404/1‑94 (MQ=60)

                CATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTT                                                                             >  SRR4302534.4883346/1‑94 (MQ=60)

                CATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTT                                                                             <  SRR4302534.2418844/94‑1 (MQ=60)

                CATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTT                                                                             >  SRR4302534.196458/1‑94 (MQ=60)

                CATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTT                                                                             >  SRR4302534.712939/1‑94 (MQ=60)

                 ATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTC                                                                            >  SRR4302534.957347/1‑94 (MQ=60)

                 ATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTC                                                                            <  SRR4302534.2286246/94‑1 (MQ=60)

                 ATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTC                                                                            <  SRR4302534.2002314/94‑1 (MQ=60)

                 ATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTC                                                                            <  SRR4302534.5705675/94‑1 (MQ=60)

                 ATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTC                                                                            <  SRR4302534.913785/94‑1 (MQ=60)

                  TGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCG                                                                           >  SRR4302534.1354617/1‑94 (MQ=60)

                  TGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCG                                                                           >  SRR4302534.4922727/1‑94 (MQ=60)

                   GATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGC                                                                          >  SRR4302534.3230744/1‑94 (MQ=60)

                    ATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCG                                                                         >  SRR4302534.412689/1‑94 (MQ=60)

                     TGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGG                                                                        >  SRR4302534.538257/1‑94 (MQ=60)

                     TGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGG                                                                        <  SRR4302534.3313800/94‑1 (MQ=60)

                       ATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTT                                                                      <  SRR4302534.1424006/94‑1 (MQ=60)

                        TGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTA                                                                     <  SRR4302534.5998344/94‑1 (MQ=60)

                        TGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTA                                                                     <  SRR4302534.25399/94‑1 (MQ=60)

                          GCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATA                                                                   <  SRR4302534.2058670/94‑1 (MQ=60)

                           CGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATAT                                                                  <  SRR4302534.2931219/94‑1 (MQ=60)

                           CGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATAT                                                                  <  SRR4302534.2817785/94‑1 (MQ=60)

                           CGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATAT                                                                  <  SRR4302534.2353827/94‑1 (MQ=60)

                           CGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATAT                                                                  <  SRR4302534.3114260/94‑1 (MQ=60)

                             CAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCA                                                                <  SRR4302534.961070/94‑1 (MQ=60)

                              AGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAG                                                               <  SRR4302534.5599523/94‑1 (MQ=60)

                              AGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAG                                                               <  SRR4302534.807848/94‑1 (MQ=60)

                               GCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGC                                                              >  SRR4302534.6156720/1‑94 (MQ=60)

                                CGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCG                                                             >  SRR4302534.3559398/1‑94 (MQ=60)

                                CGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCG                                                             >  SRR4302534.5366349/1‑94 (MQ=60)

                                 GTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGA                                                            <  SRR4302534.5774688/94‑1 (MQ=60)

                                  TTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGAT                                                           >  SRR4302534.2280265/1‑94 (MQ=60)

                                   TTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATA                                                          <  SRR4302534.930610/94‑1 (MQ=60)

                                    TGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAA                                                         >  SRR4302534.2468602/1‑94 (MQ=60)

                                    TGTATAAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAA                                                         >  SRR4302534.1172114/1‑94 (MQ=60)

                                     GTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAAT                                                        >  SRR4302534.3613205/1‑94 (MQ=60)

                                      TATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATT                                                       <  SRR4302534.2075986/94‑1 (MQ=60)

                                       ATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTT                                                      >  SRR4302534.6486538/1‑94 (MQ=60)

                                        TGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTT                                                     <  SRR4302534.5639361/94‑1 (MQ=60)

                                        TGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTT                                                     <  SRR4302534.2254572/94‑1 (MQ=60)

                                         GAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTG                                                    >  SRR4302534.1658121/1‑94 (MQ=60)

                                         GAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTG                                                    >  SRR4302534.4641974/1‑94 (MQ=60)

                                         GAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTG                                                    >  SRR4302534.5936424/1‑94 (MQ=60)

                                          AAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGG                                                   >  SRR4302534.3424178/1‑94 (MQ=60)

                                          AAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGG                                                   >  SRR4302534.4168178/1‑94 (MQ=60)

                                           AGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGT                                                  <  SRR4302534.6247240/94‑1 (MQ=60)

                                             CAGTCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAA                                                <  SRR4302534.4601673/94‑1 (MQ=60)

                                             CAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAA                                                <  SRR4302534.2159540/94‑1 (MQ=60)

                                             CAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAA                                                >  SRR4302534.1931896/1‑94 (MQ=60)

                                             CAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAA                                                <  SRR4302534.6348748/94‑1 (MQ=60)

                                             CAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAA                                                <  SRR4302534.4445125/94‑1 (MQ=60)

                                             CAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAA                                                <  SRR4302534.3071283/94‑1 (MQ=60)

                                              AGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAG                                               >  SRR4302534.3911789/1‑94 (MQ=60)

                                              AGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAG                                               <  SRR4302534.3724020/94‑1 (MQ=60)

                                              AGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAG                                               <  SRR4302534.2823001/94‑1 (MQ=60)

                                              AGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAG                                               <  SRR4302534.3301432/94‑1 (MQ=60)

                                               GGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGA                                              <  SRR4302534.4941330/94‑1 (MQ=60)

                                                GCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAA                                             >  SRR4302534.1532949/1‑94 (MQ=60)

                                                 CTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAA                                            >  SRR4302534.5522165/1‑94 (MQ=60)

                                                  TATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAAT                                           >  SRR4302534.2808268/1‑94 (MQ=60)

                                                   ATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATA                                          >  SRR4302534.5085015/1‑94 (MQ=60)

                                                   ATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATA                                          >  SRR4302534.2332181/1‑94 (MQ=60)

                                                    TATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATAT                                         >  SRR4302534.4326070/1‑94 (MQ=60)

                                                    TATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATAT                                         >  SRR4302534.2787665/1‑94 (MQ=60)

                                                     ATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATC                                        <  SRR4302534.4708640/94‑1 (MQ=60)

                                                       CACTTACATGCGTCCCGACTCCACTAACCCGAGTCAGGGCGCGGTAAATATGGTTCGCGGCTATATCAGCGATAATTTTGGTAAGAAATATCTG                                      >  SRR4302534.958121/1‑94 (MQ=60)

                                                       CACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTG                                      <  SRR4302534.5829688/94‑1 (MQ=60)

                                                       CACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTG                                      >  SRR4302534.4598302/1‑94 (MQ=60)

                                                       CACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTG                                      <  SRR4302534.247071/94‑1 (MQ=60)

                                                       CACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTG                                      <  SRR4302534.1554939/94‑1 (MQ=60)

                                                        ACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGC                                     >  SRR4302534.1273988/1‑94 (MQ=60)

                                                         CTTACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCC                                    >  SRR4302534.2791242/1‑94 (MQ=60)

                                                           TACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGG                                  >  SRR4302534.5665659/1‑94 (MQ=60)

                                                           TACATGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGG                                  <  SRR4302534.5650488/94‑1 (MQ=60)

                                                               TGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAG                              <  SRR4302534.5025299/94‑1 (MQ=60)

                                                               TGCGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAG                              <  SRR4302534.2616369/94‑1 (MQ=60)

                                                                 CGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATTAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTC                            >  SRR4302534.6159652/1‑94 (MQ=60)

                                                                 CGTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTC                            >  SRR4302534.1059897/1‑94 (MQ=60)

                                                                  GTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCC                           >  SRR4302534.1110891/1‑94 (MQ=60)

                                                                  GTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCC                           >  SRR4302534.4061715/1‑94 (MQ=60)

                                                                  GTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCC                           <  SRR4302534.2828341/94‑1 (MQ=60)

                                                                  GTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCC                           <  SRR4302534.3075000/94‑1 (MQ=60)

                                                                  GTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCC                           >  SRR4302534.4506152/1‑94 (MQ=60)

                                                                  GTACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCAGCCGGAAAGTCC                           >  SRR4302534.4878275/1‑94 (MQ=60)

                                                                   TACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCG                          <  SRR4302534.6562658/94‑1 (MQ=60)

                                                                    ACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGA                         >  SRR4302534.646674/1‑94 (MQ=60)

                                                                    ACCGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGA                         >  SRR4302534.4313697/1‑94 (MQ=60)

                                                                      CGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAT                       <  SRR4302534.659879/94‑1 (MQ=60)

                                                                      CGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAT                       <  SRR4302534.441493/94‑1 (MQ=60)

                                                                      CGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAT                       <  SRR4302534.6643568/94‑1 (MQ=60)

                                                                      CGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAT                       >  SRR4302534.567755/1‑94 (MQ=60)

                                                                      CGACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAAT                       <  SRR4302534.5066944/94‑1 (MQ=60)

                                                                       GACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATC                      >  SRR4302534.4367069/1‑94 (MQ=60)

                                                                        ACTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCA                     <  SRR4302534.6268736/94‑1 (MQ=60)

                                                                         CTCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAG                    >  SRR4302534.3472790/1‑94 (MQ=60)

                                                                          TCCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGT                   <  SRR4302534.5022776/94‑1 (MQ=60)

                                                                           CCACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTA                  <  SRR4302534.1382457/94‑1 (MQ=60)

                                                                            CACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTAC                 >  SRR4302534.4152049/1‑94 (MQ=60)

                                                                             ACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACG                <  SRR4302534.5422508/94‑1 (MQ=60)

                                                                             ACTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACG                >  SRR4302534.880856/1‑94 (MQ=60)

                                                                              CTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGC               >  SRR4302534.4924142/1‑94 (MQ=60)

                                                                              CTAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGC               >  SRR4302534.3084281/1‑94 (MQ=60)

                                                                               TAACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCC              <  SRR4302534.2283368/94‑1 (MQ=60)

                                                                                AACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCA             <  SRR4302534.263907/94‑1 (MQ=60)

                                                                                 ACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAG            <  SRR4302534.1216420/94‑1 (MQ=60)

                                                                                 ACCTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAG            <  SRR4302534.4169271/94‑1 (MQ=60)

                                                                                   CTGAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCA          >  SRR4302534.3458404/1‑94 (MQ=60)

                                                                                     GAGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAA        >  SRR4302534.2091057/1‑94 (MQ=60)

                                                                                      AGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAG       >  SRR4302534.4665380/1‑94 (MQ=60)

                                                                                      AGTCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAG       >  SRR4302534.838585/1‑94 (MQ=60)

                                                                                       GTCAGGGCGCGGTAAATATGGTTCGCGGTTTTGTCAATGATAATTTTGGTAAGAAATATCCGGCGGAAAGTCCGCATCAGTATGACATCAAAGA      >  SRR4302534.5394826/1‑94 (MQ=38)

                                                                                        TCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAA     <  SRR4302534.4767874/94‑1 (MQ=60)

                                                                                        TCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAA     >  SRR4302534.3209253/1‑94 (MQ=60)

                                                                                        TCAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAA     >  SRR4302534.3759842/1‑94 (MQ=60)

                                                                                         CAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAA    <  SRR4302534.65945/94‑1 (MQ=60)

                                                                                         CAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAA    >  SRR4302534.5287819/1‑94 (MQ=60)

                                                                                         CAGGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAA    <  SRR4302534.5452355/94‑1 (MQ=60)

                                                                                           GGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAAC  >  SRR4302534.5790616/1‑94 (MQ=60)

                                                                                           GGGCGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAAC  <  SRR4302534.4274286/94‑1 (MQ=60)

TTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAAC  >  NC_000913/1329967‑1330151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`