Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,445,862	T→C	L107P (CTG→CCG)	feaB →	phenylacetaldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,445,862	0	T	C	99.6%	859.7 / NA	247	L107P (CTG→CCG)	feaB	phenylacetaldehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (125/120); minor base G (0/1); total (126/121)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

GGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA  >  NC_000913/1445772‑1445951

ggCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCgg                                                                                         >  2:5345088/1‑93 (MQ=255)

ggCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCgg                                                                                         <  1:2443485/93‑1 (MQ=255)

ggCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCgg                                                                                         >  1:5544249/1‑93 (MQ=255)

 gCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGa                                                                                        <  2:4351440/93‑1 (MQ=255)

 gCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGa                                                                                        <  2:1291151/93‑1 (MQ=255)

 gCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGa                                                                                        <  1:5735689/93‑1 (MQ=255)

  cGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAg                                                                                       >  2:1450241/1‑93 (MQ=255)

   gATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgc                                                                                      <  1:2046764/93‑1 (MQ=255)

   gATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgc                                                                                      >  2:2823755/1‑93 (MQ=255)

       aCCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaagg                                                                                  >  1:5475068/1‑93 (MQ=255)

       aCCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaagg                                                                                  >  1:2256340/1‑93 (MQ=255)

        cccGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaaggc                                                                                 >  1:2648988/1‑93 (MQ=255)

        cccGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaaggc                                                                                 >  2:5281355/1‑93 (MQ=255)

         ccGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaaggca                                                                                <  2:4225305/93‑1 (MQ=255)

         ccGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaaggca                                                                                >  2:2552422/1‑93 (MQ=255)

         ccGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaaggca                                                                                >  1:3104814/1‑93 (MQ=255)

          cGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaaggcaa                                                                               >  2:3723604/1‑93 (MQ=255)

           gCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAgcaaggcaag                                                                              >  2:2070500/1‑93 (MQ=255)

             agagCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTc                                                                            <  2:6445921/93‑1 (MQ=255)

              gagCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCa                                                                           >  2:2647563/1‑93 (MQ=255)

              gagCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCa                                                                           >  2:3598488/1‑93 (MQ=255)

               agCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCaa                                                                          <  2:5039703/93‑1 (MQ=255)

                gCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAAt                                                                         >  2:4458248/1‑93 (MQ=255)

                 cGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAAtt                                                                        <  1:4593560/93‑1 (MQ=255)

                   tGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGc                                                                      >  2:5138981/1‑93 (MQ=255)

                   tGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGc                                                                      >  2:197853/1‑93 (MQ=255)

                    gAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGTAACCCCGGAGCAAGGCAAGCCAATTGcc                                                                     >  1:2164459/1‑93 (MQ=255)

                    gAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGcc                                                                     >  2:3391306/1‑93 (MQ=255)

                     aaCGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCa                                                                    >  2:226196/1‑93 (MQ=255)

                     aaCGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCa                                                                    >  1:881797/1‑93 (MQ=255)

                     aaCGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCa                                                                    >  2:3077930/1‑93 (MQ=255)

                      aCGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCAt                                                                   <  2:29582/93‑1 (MQ=255)

                      aCGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCAt                                                                   <  2:5603608/93‑1 (MQ=255)

                       cGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCAtt                                                                  <  1:519010/93‑1 (MQ=255)

                          aTTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTcc                                                               >  1:3673073/1‑93 (MQ=255)

                           ttCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTccc                                                              <  1:92110/93‑1 (MQ=255)

                           ttCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTccc                                                              <  1:1302513/93‑1 (MQ=255)

                            tCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCg                                                             >  1:5613952/1‑93 (MQ=255)

                             cTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGt                                                            <  2:6375972/93‑1 (MQ=255)

                             cTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGt                                                            <  2:3369097/93‑1 (MQ=255)

                              tGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGtc                                                           >  2:584349/1‑92 (MQ=255)

                                 tACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCtt                                                        >  1:182120/1‑93 (MQ=255)

                                 tACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCtt                                                        <  1:1243497/93‑1 (MQ=255)

                                  aCGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAACCCCCGGAGCAAGGCAAGTCAATTGCCAGTTCCCGTGCttt                                                       <  1:660894/93‑1 (MQ=255)

                                  aCGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCttt                                                       <  1:2469246/93‑1 (MQ=255)

                                   cGTTTTGCTGATCTGGTGGAGCATCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCtttt                                                      <  1:3996037/93‑1 (MQ=255)

                                    gTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTg                                                     <  1:1338207/93‑1 (MQ=255)

                                    gTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTg                                                     >  2:247207/1‑93 (MQ=255)

                                     ttttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGa                                                    >  1:6424438/1‑93 (MQ=255)

                                     ttttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGa                                                    >  2:109072/1‑93 (MQ=255)

                                      tttGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGaa                                                   <  1:2972464/93‑1 (MQ=255)

                                         gCTGATCTGGTGGAGCAGCACAGAGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTg                                                >  2:1735581/1‑93 (MQ=255)

                                            gATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGc                                             >  1:1014390/1‑93 (MQ=255)

                                            gATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCAGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGc                                             >  2:4660709/1‑93 (MQ=255)

                                             aTCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCt                                            >  1:5336197/1‑93 (MQ=255)

                                             aTCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCt                                            <  1:4139598/93‑1 (MQ=255)

                                             aTCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCt                                            >  1:4007685/1‑93 (MQ=255)

                                             aTCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCt                                            >  1:2708096/1‑93 (MQ=255)

                                              tCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTg                                           <  1:4551771/93‑1 (MQ=255)

                                                tggtggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTa                                         >  2:6223561/1‑93 (MQ=255)

                                                tggtggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTa                                         <  1:1083639/93‑1 (MQ=255)

                                                tggtggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTa                                         <  1:5875465/93‑1 (MQ=255)

                                                 ggtggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTAc                                        >  2:2980149/1‑93 (MQ=255)

                                                   tggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGc                                      <  1:1995702/93‑1 (MQ=255)

                                                   tggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGc                                      >  2:5432562/1‑93 (MQ=255)

                                                    ggAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCt                                     >  1:5977442/1‑93 (MQ=255)

                                                       gcagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGaa                                  >  2:4770178/1‑93 (MQ=255)

                                                       gcagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGaa                                  <  1:4705332/93‑1 (MQ=255)

                                                       gcagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGaa                                  <  1:5275336/93‑1 (MQ=255)

                                                        cagcaCAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAAc                                 <  1:3285586/93‑1 (MQ=255)

                                                          gcaCAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTg                               <  2:1583561/93‑1 (MQ=255)

                                                          gcaCAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTg                               <  2:2901142/93‑1 (MQ=255)

                                                              aGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATg                           <  2:5956888/93‑1 (MQ=255)

                                                               gTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGc                          <  2:684688/93‑1 (MQ=255)

                                                               gTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGc                          >  1:6205122/1‑93 (MQ=255)

                                                                tGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCg                         >  2:1929945/1‑93 (MQ=255)

                                                                tGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCg                         <  2:4181868/93‑1 (MQ=255)

                                                                 gaggagCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGt                        <  1:4382566/93‑1 (MQ=255)

                                                                    gagCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTtat                     <  2:6011544/93‑1 (MQ=255)

                                                                    gagCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTtat                     <  1:5926194/93‑1 (MQ=255)

                                                                     ggCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTtatc                    >  1:6077517/2‑92 (MQ=255)

                                                                        tGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccg                 >  1:3446311/1‑93 (MQ=255)

                                                                         ggCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccgc                <  2:4010032/93‑1 (MQ=255)

                                                                         ggCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccgc                >  2:3849571/1‑93 (MQ=255)

                                                                          gcgcAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccgcc               <  1:1260789/93‑1 (MQ=255)

                                                                           cgcAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccgccg              >  2:1670635/1‑93 (MQ=255)

                                                                           cgcAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAccgccg              <  2:580767/93‑1 (MQ=255)

                                                                            gcAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCgg             <  2:5549330/93‑1 (MQ=255)

                                                                            gcAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCgg             >  2:3777648/1‑93 (MQ=255)

                                                                              aaCTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGCAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGt           <  2:6468087/93‑1 (MQ=255)

                                                                              aaCTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGt           >  1:3610537/1‑93 (MQ=255)

                                                                               aCTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGAGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGtt          <  2:6082311/93‑1 (MQ=255)

                                                                                cTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTa         <  2:2491888/93‑1 (MQ=255)

                                                                                  ggAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAAc       <  1:1408722/93‑1 (MQ=255)

                                                                                   gAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACg      >  1:2837551/1‑93 (MQ=255)

                                                                                   gAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACg      >  1:3516692/1‑93 (MQ=255)

                                                                                   gAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACg      >  1:1972014/1‑93 (MQ=255)

                                                                                      aCCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGAcc   <  1:1674663/93‑1 (MQ=255)

                                                                                      aCCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGAcc   >  2:4576455/1‑93 (MQ=255)

                                                                                       ccccGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCa  >  1:94168/1‑93 (MQ=255)

GGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA  >  NC_000913/1445772‑1445951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAAT  >  NC_000913/1445769‑1445955

CCGGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCC                                                                                               <  SRR4302534.1795594/94‑1 (MQ=60)

  GGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAGCTGGAAACCCCGG                                                                                             <  SRR4302534.615771/94‑1 (MQ=60)

  GGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGG                                                                                             <  SRR4302534.2965987/94‑1 (MQ=60)

  GGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGG                                                                                             >  SRR4302534.1395377/1‑94 (MQ=60)

  GGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGG                                                                                             >  SRR4302534.5693906/1‑94 (MQ=60)

  GGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGG                                                                                             <  SRR4302534.3030047/94‑1 (MQ=60)

   GGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGA                                                                                            <  SRR4302534.2508427/94‑1 (MQ=60)

   GGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGA                                                                                            <  SRR4302534.2416331/94‑1 (MQ=60)

    GCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAG                                                                                           <  SRR4302534.5891121/94‑1 (MQ=60)

     CGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGC                                                                                          <  SRR4302534.2082128/94‑1 (MQ=60)

      GATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCA                                                                                         >  SRR4302534.623154/1‑94 (MQ=60)

      GATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCA                                                                                         <  SRR4302534.1322044/94‑1 (MQ=60)

      GATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCA                                                                                         <  SRR4302534.2100258/94‑1 (MQ=60)

       ATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAA                                                                                        >  SRR4302534.1933106/1‑94 (MQ=60)

       ATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAA                                                                                        <  SRR4302534.951493/94‑1 (MQ=60)

         TACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGG                                                                                      >  SRR4302534.5622698/1‑94 (MQ=60)

         TACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGG                                                                                      >  SRR4302534.2316156/1‑94 (MQ=60)

          ACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGC                                                                                     >  SRR4302534.2719037/1‑94 (MQ=60)

          ACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGC                                                                                     <  SRR4302534.6310814/94‑1 (MQ=60)

           CCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCA                                                                                    >  SRR4302534.3186471/1‑94 (MQ=60)

            CCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAA                                                                                   >  SRR4302534.5015517/1‑94 (MQ=60)

             CGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAG                                                                                  <  SRR4302534.5985995/94‑1 (MQ=60)

               CAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCACGGAGCAAGGCAAGTC                                                                                >  SRR4302534.540826/1‑94 (MQ=60)

                  AGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAAT                                                                             >  SRR4302534.2540/1‑94 (MQ=60)

                   GCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATT                                                                            <  SRR4302534.5666414/94‑1 (MQ=60)

                   GCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATT                                                                            <  SRR4302534.4121601/94‑1 (MQ=60)

                   GCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATT                                                                            >  SRR4302534.720651/1‑94 (MQ=60)

                    CGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTG                                                                           <  SRR4302534.4718228/94‑1 (MQ=60)

                      TGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGTAACCCCGGAGCAAGGCAAGCCAATTGCC                                                                         >  SRR4302534.2221621/1‑94 (MQ=60)

                      TGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCC                                                                         >  SRR4302534.613298/1‑94 (MQ=60)

                       GAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCA                                                                        <  SRR4302534.642737/94‑1 (MQ=60)

                       GAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCA                                                                        >  SRR4302534.904099/1‑94 (MQ=60)

                        AACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCAT                                                                       <  SRR4302534.948367/94‑1 (MQ=60)

                        AACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCAT                                                                       >  SRR4302534.410809/1‑94 (MQ=60)

                          CGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTT                                                                     >  SRR4302534.1357517/1‑94 (MQ=60)

                          CGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTT                                                                     <  SRR4302534.531913/94‑1 (MQ=60)

                          CGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTT                                                                     >  SRR4302534.5466033/1‑94 (MQ=60)

                           GTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTC                                                                    >  SRR4302534.405687/1‑94 (MQ=60)

                            TATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCC                                                                   >  SRR4302534.3771813/1‑94 (MQ=60)

                            TATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCC                                                                   >  SRR4302534.4713788/1‑94 (MQ=60)

                             ATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCC                                                                  <  SRR4302534.3328164/94‑1 (MQ=60)

                             ATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCC                                                                  <  SRR4302534.4555721/94‑1 (MQ=60)

                             ATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCC                                                                  >  SRR4302534.753894/1‑94 (MQ=60)

                              TTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCG                                                                 <  SRR4302534.1336866/94‑1 (MQ=60)

                              TTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCG                                                                 <  SRR4302534.94572/94‑1 (MQ=60)

                              TTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCG                                                                 >  SRR4302534.5765842/1‑94 (MQ=60)

                                CTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTG                                                               >  SRR4302534.5651556/1‑94 (MQ=60)

                                CTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTG                                                               <  SRR4302534.2138781/94‑1 (MQ=60)

                                CGGCTGCGTTTTGCTGAGCTGGTGGAGCAGCACAGTGAGGAGCTGGCGAAACTGGAAACCCGGGAGCAAGGCAAGTCAATTGCCATTTCCCGTG                                                               <  SRR4302534.3876483/94‑1 (MQ=60)

                                  GCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCT                                                             <  SRR4302534.2875445/94‑1 (MQ=60)

                                  GCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCT                                                             <  SRR4302534.2283458/94‑1 (MQ=60)

                                   CTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTT                                                            >  SRR4302534.2685294/1‑94 (MQ=60)

                                   CTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTT                                                            <  SRR4302534.1824580/94‑1 (MQ=60)

                                   CTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTT                                                            >  SRR4302534.186909/1‑94 (MQ=60)

                                    TACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTT                                                           <  SRR4302534.1276271/94‑1 (MQ=60)

                                     ACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAACCCCCGGAGCAAGGCAAGTCAATTGCCAGTTCCCGTGCTTTT                                                          <  SRR4302534.676962/94‑1 (MQ=60)

                                     ACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTT                                                          <  SRR4302534.2534887/94‑1 (MQ=60)

                                      CGTTTTGCTGATCTGGTGGAGCATCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTG                                                         <  SRR4302534.4103011/94‑1 (MQ=60)

                                      CGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTG                                                         <  SRR4302534.5702658/94‑1 (MQ=60)

                                       GTTTTGCTGATCTGGTTGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGA                                                        >  SRR4302534.3556620/1‑94 (MQ=60)

                                       GTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGA                                                        <  SRR4302534.1373481/94‑1 (MQ=60)

                                       GTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGA                                                        >  SRR4302534.6597698/1‑94 (MQ=60)

                                        TTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAA                                                       >  SRR4302534.4753781/1‑94 (MQ=60)

                                         TTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAG                                                      <  SRR4302534.3050288/94‑1 (MQ=60)

                                         TTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAG                                                      >  SRR4302534.3464748/1‑94 (MQ=60)

                                         TTTGCGGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCTCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAG                                                      <  SRR4302534.1208935/94‑1 (MQ=60)

                                            GCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGG                                                   <  SRR4302534.1289193/94‑1 (MQ=60)

                                            GCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGG                                                   <  SRR4302534.1156183/94‑1 (MQ=60)

                                             CTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGG                                                  <  SRR4302534.5845583/94‑1 (MQ=60)

                                              TGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGC                                                 >  SRR4302534.1040495/1‑94 (MQ=60)

                                              TGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGC                                                 >  SRR4302534.1429525/1‑94 (MQ=60)

                                               GATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCT                                                <  SRR4302534.6634373/94‑1 (MQ=60)

                                               GATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCT                                                >  SRR4302534.2779601/1‑94 (MQ=60)

                                               GATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCT                                                >  SRR4302534.5480079/1‑94 (MQ=60)

                                               GATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCT                                                >  SRR4302534.4115000/1‑94 (MQ=60)

                                                ATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTG                                               <  SRR4302534.4135429/94‑1 (MQ=60)

                                                ATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTG                                               <  SRR4302534.4251314/94‑1 (MQ=60)

                                                ATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTG                                               >  SRR4302534.4405222/1‑94 (MQ=60)

                                                 TCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGT                                              <  SRR4302534.4675155/94‑1 (MQ=60)

                                                   TGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTAC                                            <  SRR4302534.1111822/94‑1 (MQ=60)

                                                   TGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTAC                                            <  SRR4302534.6034982/94‑1 (MQ=60)

                                                   TGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTAC                                            >  SRR4302534.2727447/1‑94 (MQ=60)

                                                    GGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACG                                           <  SRR4302534.540332/94‑1 (MQ=60)

                                                    GGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACG                                           >  SRR4302534.6474571/1‑94 (MQ=60)

                                                      TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                         >  SRR4302534.2052299/1‑94 (MQ=60)

                                                      TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                         <  SRR4302534.2047972/94‑1 (MQ=60)

                                                      TGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCT                                         >  SRR4302534.6139786/1‑94 (MQ=60)

                                                       GGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTG                                        >  SRR4302534.2977114/1‑94 (MQ=60)

                                                         AGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAA                                      <  SRR4302534.6104034/94‑1 (MQ=60)

                                                          GCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAAC                                     <  SRR4302534.4833061/94‑1 (MQ=60)

                                                          GCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAAC                                     <  SRR4302534.5417798/94‑1 (MQ=60)

                                                           CAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACT                                    >  SRR4302534.5209085/1‑94 (MQ=60)

                                                           CAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACT                                    <  SRR4302534.3182650/94‑1 (MQ=60)

                                                           CAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACT                                    <  SRR4302534.3373180/94‑1 (MQ=60)

                                                               ACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGAT                                <  SRR4302534.1614064/94‑1 (MQ=60)

                                                               ACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGAT                                >  SRR4302534.4648609/1‑94 (MQ=60)

                                                               ACAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGAT                                >  SRR4302534.2714284/1‑94 (MQ=60)

                                                                CAGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATG                               >  SRR4302534.6044737/1‑94 (MQ=60)

                                                                 AGTGAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGC                              >  SRR4302534.6373843/1‑94 (MQ=60)

                                                                    GAGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTT                           <  SRR4302534.4500712/94‑1 (MQ=60)

                                                                     AGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTA                          >  SRR4302534.6092494/1‑94 (MQ=60)

                                                                     AGGAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTA                          <  SRR4302534.2855143/94‑1 (MQ=60)

                                                                       GGGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATC                        >  SRR4302534.6242789/1‑94 (MQ=60)

                                                                       GAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATA                        <  SRR4302534.6087113/94‑1 (MQ=60)

                                                                       GAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATA                        <  SRR4302534.5569395/94‑1 (MQ=60)

                                                                       GAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATA                        <  SRR4302534.1611358/94‑1 (MQ=60)

                                                                       GAGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATA                        >  SRR4302534.1946638/1‑94 (MQ=60)

                                                                        CGCTGGCGCAACTGGAAACCCCGGAGCAAGGGAAGTCAATTGCCATTTCCCGTGCTGTTTAAGTGGGCTGTACGCTGAACTGGATGCGTTATAC                       >  SRR4302534.6395342/1‑94 (MQ=60)

                                                                        AGCTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATAC                       >  SRR4302534.2040836/1‑94 (MQ=60)

                                                                          CTGGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCG                     >  SRR4302534.3538934/1‑94 (MQ=60)

                                                                            GGCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCC                   <  SRR4302534.3744660/94‑1 (MQ=60)

                                                                             GCGCAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCG                  <  SRR4302534.1293990/94‑1 (MQ=60)

                                                                                CAACTTGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGT               <  SRR4302534.2362422/94‑1 (MQ=60)

                                                                                CAACTGGAACCCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGT               <  SRR4302534.2294448/94‑1 (MQ=60)

                                                                                CAACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGT               >  SRR4302534.3707628/1‑94 (MQ=60)

                                                                                 AACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTT              <  SRR4302534.12998/94‑1 (MQ=60)

                                                                                  ACTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTA             <  SRR4302534.4807225/94‑1 (MQ=60)

                                                                                   CTGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAA            >  SRR4302534.5701023/1‑94 (MQ=60)

                                                                                    TGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAAC           >  SRR4302534.3207675/1‑94 (MQ=60)

                                                                                    TGGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAAC           <  SRR4302534.3605841/94‑1 (MQ=60)

                                                                                     GGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACG          >  SRR4302534.2912114/1‑94 (MQ=60)

                                                                                     GGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACG          <  SRR4302534.1445785/94‑1 (MQ=60)

                                                                                     GGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACG          >  SRR4302534.3611281/1‑94 (MQ=60)

                                                                                     GGAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACG          >  SRR4302534.2023636/1‑94 (MQ=60)

                                                                                      GAAACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGA         >  SRR4302534.940269/1‑94 (MQ=60)

                                                                                        AACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACC       <  SRR4302534.1069865/94‑1 (MQ=60)

                                                                                        AACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACC       >  SRR4302534.3654091/1‑94 (MQ=60)

                                                                                         ACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA      >  SRR4302534.96682/1‑94 (MQ=60)

                                                                                         ACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA      >  SRR4302534.6019310/1‑94 (MQ=60)

                                                                                         ACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA      <  SRR4302534.3732332/94‑1 (MQ=60)

                                                                                         ACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA      >  SRR4302534.3018219/1‑94 (MQ=60)

                                                                                         ACCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCA      <  SRR4302534.1718239/94‑1 (MQ=60)

                                                                                          CCCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAA     >  SRR4302534.4949345/1‑94 (MQ=60)

                                                                                           CCCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAA    >  SRR4302534.1034757/1‑94 (MQ=60)

                                                                                            CCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAA   >  SRR4302534.802274/1‑94 (MQ=60)

                                                                                            CCGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAA   >  SRR4302534.2301292/1‑94 (MQ=60)

                                                                                             CGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAAT  <  SRR4302534.3303386/94‑1 (MQ=60)

                                                                                             CGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAAT  >  SRR4302534.1436799/1‑94 (MQ=60)

CCGGGCGATTACCCGCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAGCTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAAGTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAAT  >  NC_000913/1445769‑1445955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`