Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,456,536 A→G S83S (TCA→TCG)  b1394 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,456,5360AG100.0% 928.1 / NA 269S83S (TCA→TCG) b1394hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (135/134);  total (135/134)

Only 100 of 278 total aligned reads displayed.

TACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC  >  NC_000913/1456446‑1456625
                                                                                          |                                                                                         
tacAGGTGCCGGGCGCCGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGt                                                                                         <  1:3855999/93‑1 (MQ=255)
  cAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTg                                                                                       >  2:7285076/1‑93 (MQ=255)
  cAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTg                                                                                       <  2:29411/93‑1 (MQ=255)
    ggTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGaa                                                                                     >  2:212393/1‑93 (MQ=255)
    ggTGCCGGGCGCGGATTTTGTGCCTGTTAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGaa                                                                                     <  2:6300098/93‑1 (MQ=255)
     gTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAAc                                                                                    <  2:6630520/93‑1 (MQ=255)
       gCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGt                                                                                  >  2:6175647/1‑93 (MQ=255)
        ccGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGtt                                                                                 >  2:4685635/1‑93 (MQ=255)
          gggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTc                                                                               <  1:1016973/93‑1 (MQ=255)
           ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCt                                                                              >  1:3888622/1‑93 (MQ=255)
            gcgcGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCta                                                                             <  1:1917650/93‑1 (MQ=255)
            gcgcGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCta                                                                             >  2:8748438/1‑93 (MQ=255)
             cgcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCtat                                                                            <  1:2687468/93‑1 (MQ=255)
             cgcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCtat                                                                            <  2:7142857/93‑1 (MQ=255)
                ggATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGCATGTCGGTTGAACGTTTCTATCAc                                                                         >  2:2909981/1‑93 (MQ=255)
                  aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAccc                                                                       <  1:5126705/93‑1 (MQ=255)
                  aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAccc                                                                       <  2:4660993/93‑1 (MQ=255)
                  aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAccc                                                                       >  2:8562373/1‑93 (MQ=255)
                   ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCa                                                                      <  2:5726999/93‑1 (MQ=255)
                   ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCa                                                                      <  1:5422284/93‑1 (MQ=255)
                   ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCa                                                                      >  2:5697604/1‑93 (MQ=255)
                     ttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACt                                                                    <  1:285924/93‑1 (MQ=255)
                       gtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTgg                                                                  <  1:1500931/93‑1 (MQ=255)
                       gtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCAGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTgg                                                                  <  1:7115737/93‑1 (MQ=255)
                         gCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTa                                                                <  1:6448318/93‑1 (MQ=255)
                         gCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTa                                                                >  1:7524622/1‑93 (MQ=255)
                           tGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACg                                                              >  1:811232/1‑93 (MQ=255)
                           tGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACg                                                              <  2:1839744/93‑1 (MQ=255)
                             gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTc                                                            <  1:5147925/93‑1 (MQ=255)
                             gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTc                                                            <  1:4322791/93‑1 (MQ=255)
                                aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                         >  2:1765736/1‑93 (MQ=255)
                                aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                         <  2:1901240/93‑1 (MQ=255)
                                 ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                        >  1:8388903/1‑93 (MQ=255)
                                 ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                        >  2:1984007/1‑93 (MQ=255)
                                  gATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTg                                                       <  2:5305400/93‑1 (MQ=255)
                                  gATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTg                                                       <  2:6157384/93‑1 (MQ=255)
                                   aTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTgg                                                      >  1:3327931/1‑93 (MQ=255)
                                   aTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATATCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTgg                                                      <  2:75110/93‑1 (MQ=255)
                                    tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                     >  2:7041279/1‑93 (MQ=255)
                                      ttAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCaa                                                   <  2:654929/93‑1 (MQ=255)
                                       tAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCaaa                                                  >  1:1714890/1‑93 (MQ=255)
                                           gATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTg                                              <  1:1286173/93‑1 (MQ=255)
                                           gATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTg                                              >  1:5576799/1‑93 (MQ=255)
                                             tCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGcc                                            <  1:4991510/93‑1 (MQ=255)
                                             tCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGcc                                            >  2:5586962/1‑93 (MQ=255)
                                              cGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCa                                           >  1:4522565/1‑93 (MQ=255)
                                              cGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCa                                           <  1:4089985/93‑1 (MQ=255)
                                               gTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCaa                                          >  2:8028346/1‑93 (MQ=255)
                                                tAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCaaa                                         <  2:8244869/93‑1 (MQ=255)
                                                 aaCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCaaaa                                        <  2:4911219/93‑1 (MQ=255)
                                                   cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                      >  1:1767083/1‑93 (MQ=255)
                                                   cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                      >  1:6607421/1‑93 (MQ=255)
                                                   agTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                      <  1:6911655/92‑1 (MQ=255)
                                                    gTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcag                                     >  1:903564/1‑91 (MQ=255)
                                                    gTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTAGCCTGGCAAAACTGCCAAAACCg                                     >  2:4738387/1‑93 (MQ=255)
                                                     ttGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                                    <  1:4720925/93‑1 (MQ=255)
                                                     ttGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                                    >  2:6373004/1‑93 (MQ=255)
                                                     ttGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                                    >  1:6199703/1‑93 (MQ=255)
                                                      tGATCCCACAGGCCCCGCACCGGCTTTAGGGATGTCGGTTGAACGTTTCTATACCCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGt                                   <  2:4769027/93‑1 (MQ=255)
                                                         tCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTGTATAACCCACTGGTACGGCGCGTGGCAAAACTGCCACAACCGGTGAt                                >  1:5952581/1‑93 (MQ=255)
                                                         tCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAt                                >  1:8639803/1‑93 (MQ=255)
                                                         acccACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAt                                <  1:3222649/92‑1 (MQ=255)
                                                          cccACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATc                               <  2:1291944/93‑1 (MQ=255)
                                                          cccACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATc                               >  1:3664344/1‑93 (MQ=255)
                                                            cacaGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                             >  2:3170565/1‑93 (MQ=255)
                                                              caGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgtg                           >  1:1100366/1‑93 (MQ=255)
                                                              caGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgtg                           >  1:8130362/1‑93 (MQ=255)
                                                               aGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                          <  2:42822/93‑1 (MQ=255)
                                                                ggCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                         >  2:2295215/1‑93 (MQ=255)
                                                                ggCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                         <  2:5976740/93‑1 (MQ=255)
                                                                 gCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAg                        <  2:2506454/93‑1 (MQ=255)
                                                                 gCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAg                        <  2:5901264/93‑1 (MQ=255)
                                                                   cccGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTc                      >  1:1475039/1‑93 (MQ=255)
                                                                   cccGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTc                      <  1:2465051/93‑1 (MQ=255)
                                                                     cGCACCGGATTTAGGGATGTCGGTTTAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCaa                    <  2:4010216/93‑1 (MQ=255)
                                                                     cGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCaa                    <  2:3751791/93‑1 (MQ=255)
                                                                     cGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCaa                    <  1:67945/93‑1 (MQ=255)
                                                                     cGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCaa                    <  1:8721423/93‑1 (MQ=255)
                                                                     cGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCaa                    <  2:361751/93‑1 (MQ=255)
                                                                      gCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt                   >  2:3710300/1‑93 (MQ=255)
                                                                       cACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAGACTGCCAAAACCGGTGATCTGTGCAGTCAAtg                  <  1:5244314/93‑1 (MQ=255)
                                                                       cACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtg                  <  1:7428692/93‑1 (MQ=255)
                                                                        aCCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtgg                 >  2:7635209/1‑93 (MQ=255)
                                                                         ccGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc                >  1:7967902/1‑93 (MQ=255)
                                                                          cGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg               >  1:6169955/1‑93 (MQ=255)
                                                                           ggATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt              >  2:3333790/1‑93 (MQ=255)
                                                                           ggATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt              >  2:671032/1‑93 (MQ=255)
                                                                            gATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtg             >  1:2225826/1‑93 (MQ=255)
                                                                            gATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtg             <  1:4204596/93‑1 (MQ=255)
                                                                              tttAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggc           <  1:4283359/93‑1 (MQ=255)
                                                                               ttAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggcg          >  2:6432076/1‑93 (MQ=255)
                                                                                   ggATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcag      <  1:481713/93‑1 (MQ=255)
                                                                                   ggATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcag      >  1:6513714/1‑93 (MQ=255)
                                                                                   ggATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcag      <  1:2254318/93‑1 (MQ=255)
                                                                                    gATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagg     >  1:7765204/1‑93 (MQ=255)
                                                                                    gATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagg     >  2:4554557/1‑93 (MQ=255)
                                                                                      tGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagggg   <  2:2712067/93‑1 (MQ=255)
                                                                                      tGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagggg   <  1:3556037/93‑1 (MQ=255)
                                                                                      tGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagggg   >  1:1320290/1‑93 (MQ=255)
                                                                                       gTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcaggggc  >  2:2427482/1‑93 (MQ=255)
                                                                                          |                                                                                         
TACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC  >  NC_000913/1456446‑1456625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGC  >  NC_000913/1456443‑1456629
                                                                                             |                                                                                             
TCTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCG                                                                                               <  SRR4302541.6883226/94‑1 (MQ=60)
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCG                                                                                               <  SRR4302541.234694/94‑1 (MQ=60)
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCG                                                                                               <  SRR4302541.301712/94‑1 (MQ=60)
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCG                                                                                               <  SRR4302541.5211209/94‑1 (MQ=60)
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCG                                                                                               <  SRR4302541.6535777/94‑1 (MQ=60)
 CTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGG                                                                                              <  SRR4302541.1282522/94‑1 (MQ=60)
   TACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTT                                                                                            >  SRR4302541.6236027/1‑94 (MQ=60)
   TACAGGTGCCGGGCGCCGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTT                                                                                            <  SRR4302541.3894794/94‑1 (MQ=60)
   TACAGGTGCCGGGCCGGGATTTTGTGCTTGTCAACAGCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTT                                                                                            <  SRR4302541.3629865/94‑1 (MQ=60)
    ACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTG                                                                                           <  SRR4302541.7710140/94‑1 (MQ=60)
    ACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTG                                                                                           >  SRR4302541.627724/1‑94 (MQ=60)
     CAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGA                                                                                          >  SRR4302541.5457728/1‑94 (MQ=60)
     CAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGA                                                                                          <  SRR4302541.148998/94‑1 (MQ=60)
       GGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAAC                                                                                        <  SRR4302541.5259230/94‑1 (MQ=60)
            CGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTC                                                                                   <  SRR4302541.7543763/94‑1 (MQ=60)
             GGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCT                                                                                  <  SRR4302541.1026375/94‑1 (MQ=60)
             GGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCT                                                                                  >  SRR4302541.3927618/1‑94 (MQ=60)
             GGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCT                                                                                  >  SRR4302541.4237661/1‑94 (MQ=60)
             CGGCGCGGATTTTGGTCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCT                                                                                  <  SRR4302541.7307031/94‑1 (MQ=60)
              GGCGCTGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTA                                                                                 <  SRR4302541.4564236/94‑1 (MQ=60)
               GCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTAT                                                                                <  SRR4302541.4920149/94‑1 (MQ=60)
               GCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTAT                                                                                <  SRR4302541.4920148/94‑1 (MQ=60)
               GCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTAT                                                                                <  SRR4302541.1936096/94‑1 (MQ=60)
                CGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATA                                                                               <  SRR4302541.2714159/94‑1 (MQ=60)
                 GCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAA                                                                              <  SRR4302541.8791285/94‑1 (MQ=60)
                   GGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACC                                                                            <  SRR4302541.8847597/94‑1 (MQ=60)
                     ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCA                                                                          >  SRR4302541.2443935/1‑94 (MQ=60)
                     ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCA                                                                          <  SRR4302541.5179404/94‑1 (MQ=60)
                     ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCA                                                                          <  SRR4302541.8447104/94‑1 (MQ=60)
                     ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCA                                                                          <  SRR4302541.7975270/94‑1 (MQ=60)
                     ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCA                                                                          <  SRR4302541.2253228/94‑1 (MQ=60)
                      TTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCAC                                                                         <  SRR4302541.5477916/94‑1 (MQ=60)
                      TTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCAC                                                                         >  SRR4302541.4143299/1‑94 (MQ=60)
                        TTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTG                                                                       <  SRR4302541.288370/94‑1 (MQ=60)
                        TTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTG                                                                       >  SRR4302541.3449737/1‑94 (MQ=60)
                         TGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGG                                                                      <  SRR4302541.7748909/94‑1 (MQ=60)
                         TGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACACACTGG                                                                      >  SRR4302541.1723845/1‑94 (MQ=60)
                          GTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGT                                                                     <  SRR4302541.1515785/94‑1 (MQ=60)
                          GTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGT                                                                     <  SRR4302541.6609153/94‑1 (MQ=60)
                          GTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCAGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGT                                                                     <  SRR4302541.7191529/94‑1 (MQ=60)
                           TGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTtgtc                                                                    >  SRR4302541.3678683/1‑90 (MQ=60)
                           TGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTA                                                                    >  SRR4302541.7605023/1‑94 (MQ=60)
                            GCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTAC                                                                   >  SRR4302541.8212504/1‑94 (MQ=60)
                            GCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTAC                                                                   <  SRR4302541.6514035/94‑1 (MQ=60)
                             CTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACG                                                                  >  SRR4302541.818655/1‑94 (MQ=60)
                              TGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGT                                                                 <  SRR4302541.4105402/94‑1 (MQ=60)
                              TGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGT                                                                 >  SRR4302541.6934756/1‑94 (MQ=60)
                               GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTC                                                                >  SRR4302541.2371562/1‑94 (MQ=60)
                                GTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCG                                                               >  SRR4302541.820154/1‑94 (MQ=60)
                                GTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCG                                                               <  SRR4302541.2772554/94‑1 (MQ=60)
                                GTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCG                                                               <  SRR4302541.5200882/94‑1 (MQ=60)
                                GTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCG                                                               <  SRR4302541.4365948/94‑1 (MQ=60)
                                  CAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCC                                                             <  SRR4302541.2979600/94‑1 (MQ=60)
                                  CAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCC                                                             <  SRR4302541.7168811/94‑1 (MQ=60)
                                   AGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCT                                                            >  SRR4302541.8479733/1‑94 (MQ=60)
                                     GATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGG                                                          >  SRR4302541.3361270/1‑94 (MQ=60)
                                     GATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGCCGCCTGC                                                          >  SRR4302541.7565285/1‑94 (MQ=60)
                                      ATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGC                                                         <  SRR4302541.4361603/94‑1 (MQ=60)
                                      ATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGA                                                         >  SRR4302541.7282885/1‑94 (MQ=60)
                                        CTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAA                                                       >  SRR4302541.3732038/1‑94 (MQ=60)
                                         TTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAA                                                      >  SRR4302541.4698911/1‑94 (MQ=60)
                                         TTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAA                                                      >  SRR4302541.1731670/1‑94 (MQ=60)
                                          TAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAA                                                     >  SRR4302541.5304819/1‑94 (MQ=60)
                                           AACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAC                                                    >  SRR4302541.1133211/1‑94 (MQ=60)
                                           AACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAC                                                    <  SRR4302541.2129057/94‑1 (MQ=60)
                                             CGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTCTCACACACTGGTACGTCGCCTGGCAAAACTG                                                  >  SRR4302541.142670/1‑94 (MQ=60)
                                             CGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTG                                                  >  SRR4302541.5633886/1‑94 (MQ=60)
                                              GATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGC                                                 <  SRR4302541.1298907/94‑1 (MQ=60)
                                               ATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCC                                                <  SRR4302541.1918081/94‑1 (MQ=60)
                                                TCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCA                                               <  SRR4302541.5042752/94‑1 (MQ=60)
                                                TCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCA                                               >  SRR4302541.4568209/1‑94 (MQ=60)
                                                ACGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCA                                               <  SRR4302541.593079/94‑1 (MQ=60)
                                                 CGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAA                                              <  SRR4302541.3683336/94‑1 (MQ=60)
                                                 CGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAA                                              <  SRR4302541.4130365/94‑1 (MQ=60)
                                                   TAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAA                                            <  SRR4302541.4001148/94‑1 (MQ=60)
                                                   TAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAA                                            <  SRR4302541.5183871/94‑1 (MQ=60)
                                                     ACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACC                                          >  SRR4302541.6675123/1‑94 (MQ=60)
                                                     ACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACC                                          >  SRR4302541.42976/1‑94 (MQ=60)
                                                     ACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACC                                          <  SRR4302541.4256936/94‑1 (MQ=60)
                                                     ACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACC                                          >  SRR4302541.1784268/1‑94 (MQ=60)
                                                      CGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCG                                         <  SRR4302541.4456101/94‑1 (MQ=60)
                                                      CGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACAG                                         >  SRR4302541.911947/1‑94 (MQ=60)
                                                      AGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCG                                         <  SRR4302541.6985407/94‑1 (MQ=60)
                                                       GTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAATACCGG                                        >  SRR4302541.5600059/1‑94 (MQ=60)
                                                       GTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGG                                        >  SRR4302541.6263051/1‑94 (MQ=60)
                                                       GTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGG                                        <  SRR4302541.4799831/94‑1 (MQ=60)
                                                       GTGGATCCCACAGGCCCCGCACCGTATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGG                                        <  SRR4302541.6089329/94‑1 (MQ=60)
                                                        TTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGT                                       >  SRR4302541.5252914/1‑94 (MQ=60)
                                                        TTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGT                                       <  SRR4302541.4769070/94‑1 (MQ=60)
                                                        TTGATCCCACAGGCCCCGCACCGGATTGAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGT                                       <  SRR4302541.517482/94‑1 (MQ=60)
                                                         TGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTG                                      <  SRR4302541.6559726/94‑1 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGA                                     >  SRR4302541.6878586/1‑94 (MQ=60)
                                                           ATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTGTATAACCCACTGGTACGGCGCGTGGCAAAACTGCCACAACCGGTGAT                                    >  SRR4302541.6013246/1‑94 (MQ=60)
                                                           ATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAT                                    <  SRR4302541.5138143/94‑1 (MQ=60)
                                                           ATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAT                                    >  SRR4302541.8732352/1‑94 (MQ=60)
                                                           ATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAT                                    <  SRR4302541.6864987/94‑1 (MQ=60)
                                                            TCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATC                                   >  SRR4302541.3701520/1‑94 (MQ=60)
                                                            ACCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATC                                   <  SRR4302541.3255202/94‑1 (MQ=60)
                                                             CCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCT                                  >  SRR4302541.6242681/1‑94 (MQ=60)
                                                              CCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAACACCGGTGATCTG                                 >  SRR4302541.6599886/1‑94 (MQ=60)
                                                              CCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTG                                 >  SRR4302541.3923873/1‑94 (MQ=60)
                                                                CCAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTG                               <  SRR4302541.1844862/94‑1 (MQ=60)
                                                                ACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTG                               <  SRR4302541.5636796/94‑1 (MQ=60)
                                                                ACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTG                               >  SRR4302541.8219360/1‑94 (MQ=60)
                                                                ACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTG                               >  SRR4302541.8334604/1‑94 (MQ=60)
                                                                ACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTG                               >  SRR4302541.1110666/1‑94 (MQ=60)
                                                                 CAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGC                              <  SRR4302541.6299740/94‑1 (MQ=60)
                                                                  AGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCG                             >  SRR4302541.1450471/1‑94 (MQ=60)
                                                                  AGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCA                             >  SRR4302541.3352099/1‑94 (MQ=60)
                                                                  AGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCA                             >  SRR4302541.8139731/1‑94 (MQ=60)
                                                                   GGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAG                            <  SRR4302541.6174267/94‑1 (MQ=60)
                                                                    GCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGT                           <  SRR4302541.2095057/94‑1 (MQ=60)
                                                                     CCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTC                          >  SRR4302541.1489506/1‑94 (MQ=60)
                                                                      CCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCA                         <  SRR4302541.2489679/94‑1 (MQ=60)
                                                                       CCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAA                        <  SRR4302541.86742/94‑1 (MQ=60)
                                                                        CGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAT                       <  SRR4302541.8814689/94‑1 (MQ=60)
                                                                        CGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAT                       >  SRR4302541.3256388/1‑94 (MQ=60)
                                                                        CGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAT                       <  SRR4302541.68508/94‑1 (MQ=60)
                                                                          CACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAGACTGCCAAAACCGGTGATCTGTGCAGTCAATGG                     <  SRR4302541.5298355/94‑1 (MQ=60)
                                                                          CACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGG                     <  SRR4302541.7508113/94‑1 (MQ=60)
                                                                          CACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGG                     >  SRR4302541.1751883/1‑94 (MQ=60)
                                                                           ACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGC                    >  SRR4302541.8055718/1‑94 (MQ=60)
                                                                            CCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCG                   >  SRR4302541.6232993/1‑94 (MQ=60)
                                                                              GGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG                 <  SRR4302541.8766217/94‑1 (MQ=60)
                                                                              GGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG                 >  SRR4302541.2247395/1‑94 (MQ=60)
                                                                              GGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG                 >  SRR4302541.2586536/1‑94 (MQ=60)
                                                                               GATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGG                >  SRR4302541.1537892/1‑94 (MQ=60)
                                                                               GATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGG                <  SRR4302541.4246399/94‑1 (MQ=60)
                                                                                 TTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCG              <  SRR4302541.4326137/94‑1 (MQ=60)
                                                                                 TTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCG              <  SRR4302541.1064609/94‑1 (MQ=60)
                                                                                    AGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA           <  SRR4302541.6701920/94‑1 (MQ=60)
                                                                                     GGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAG          >  SRR4302541.6580182/1‑94 (MQ=60)
                                                                                      GGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG         <  SRR4302541.2276090/94‑1 (MQ=60)
                                                                                      GGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG         <  SRR4302541.1309530/94‑1 (MQ=60)
                                                                                      GGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG         <  SRR4302541.4759184/94‑1 (MQ=60)
                                                                                      GGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG         >  SRR4302541.7847815/1‑94 (MQ=60)
                                                                                      GGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG         <  SRR4302541.486273/94‑1 (MQ=60)
                                                                                      GGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGCGATCTGTGCAGTCAATGGCGTGGCGGCACG         >  SRR4302541.7847816/1‑94 (MQ=60)
                                                                                       GATGTCGGTTGACCGTTTCTATAACCCACCGGTCCGTCGCCTGGCAAAACTGCCAAACCCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG        <  SRR4302541.2886997/94‑1 (MQ=60)
                                                                                       GATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG        >  SRR4302541.1233505/1‑94 (MQ=60)
                                                                                       GATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG        <  SRR4302541.8710740/94‑1 (MQ=60)
                                                                                       GATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG        <  SRR4302541.2496893/94‑1 (MQ=60)
                                                                                       GATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG        <  SRR4302541.3564283/94‑1 (MQ=60)
                                                                                       GATGTCGGTTGAACGTTGCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG        <  SRR4302541.5137413/94‑1 (MQ=60)
                                                                                        ATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG       >  SRR4302541.1333277/1‑94 (MQ=60)
                                                                                        ATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG       >  SRR4302541.4820215/1‑94 (MQ=60)
                                                                                        ATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG       >  SRR4302541.1774157/1‑94 (MQ=60)
                                                                                         TGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC      >  SRR4302541.1314536/1‑94 (MQ=60)
                                                                                         TGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC      <  SRR4302541.3592007/94‑1 (MQ=60)
                                                                                         TGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC      >  SRR4302541.3817615/1‑94 (MQ=60)
                                                                                           TCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAG    >  SRR4302541.3531962/1‑94 (MQ=60)
                                                                                            CGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGG   >  SRR4302541.1462947/1‑94 (MQ=60)
                                                                                             GGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGC  >  SRR4302541.8616161/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGC  >  NC_000913/1456443‑1456629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑