Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,456,536 A→G S83S (TCA→TCG)  b1394 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,456,5360AG99.5% 754.7 / ‑4.6 219S83S (TCA→TCG) b1394hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (107/111);  total (108/111)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 223 total aligned reads displayed.

TACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC  >  NC_000913/1456446‑1456625
                                                                                          |                                                                                         
tacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGt                                                                                         <  2:1200158/93‑1 (MQ=255)
   aGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGa                                                                                      >  2:5899271/1‑93 (MQ=255)
    ggTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGaa                                                                                     >  1:1020850/1‑93 (MQ=255)
      tGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACg                                                                                   <  1:3281753/93‑1 (MQ=255)
       gCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGt                                                                                  <  1:3838342/93‑1 (MQ=255)
        ccGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGtt                                                                                 <  1:689012/93‑1 (MQ=255)
          gggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTc                                                                               <  2:2820329/93‑1 (MQ=255)
          gggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTc                                                                               <  1:617634/93‑1 (MQ=255)
           ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCt                                                                              >  1:6223174/1‑93 (MQ=255)
           ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCt                                                                              <  2:2283755/93‑1 (MQ=255)
            gcgcGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCta                                                                             <  1:5007777/93‑1 (MQ=255)
              gcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGCTTTAGGGATGTCGGTTGAACGTTTCtata                                                                           >  1:1572534/1‑93 (MQ=255)
               cgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATaa                                                                          <  2:185574/93‑1 (MQ=255)
                 gATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAcc                                                                        <  2:2110652/93‑1 (MQ=255)
                  aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAccc                                                                       >  2:4314038/1‑93 (MQ=255)
                  aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAccc                                                                       >  1:2880774/1‑93 (MQ=255)
                  aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAccc                                                                       <  2:516937/93‑1 (MQ=255)
                   ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCa                                                                      >  1:1720746/1‑93 (MQ=255)
                      tgtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTg                                                                   <  1:1092980/93‑1 (MQ=255)
                       gtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTgg                                                                  >  1:3135810/1‑93 (MQ=255)
                       gtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTgg                                                                  <  1:5396260/93‑1 (MQ=255)
                        tgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGt                                                                 <  2:1518200/93‑1 (MQ=255)
                        tgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGt                                                                 <  1:5282317/93‑1 (MQ=255)
                          cTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTAc                                                               <  1:5547565/93‑1 (MQ=255)
                          cTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTAc                                                               <  2:3060927/93‑1 (MQ=255)
                          cTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTAc                                                               >  2:3831255/1‑93 (MQ=255)
                           tGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACg                                                              >  1:4269351/1‑93 (MQ=255)
                            ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTTGTACGt                                                             <  1:5362265/93‑1 (MQ=255)
                            ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGt                                                             <  2:2648257/93‑1 (MQ=255)
                            ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGt                                                             <  1:6287047/93‑1 (MQ=255)
                            ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGt                                                             <  2:538690/93‑1 (MQ=255)
                            ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGt                                                             >  2:344850/1‑93 (MQ=255)
                             gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTc                                                            <  2:1275769/93‑1 (MQ=255)
                             gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTc                                                            >  1:704377/1‑93 (MQ=255)
                             gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGCCGGTTGAACGTTTCTATAACCCACTGGTACGTc                                                            <  2:1754553/93‑1 (MQ=255)
                                aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                         >  2:4805429/1‑93 (MQ=255)
                                aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                         >  1:4817388/1‑93 (MQ=255)
                                 ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                        <  2:2272208/93‑1 (MQ=255)
                                 ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                        >  2:1064626/1‑93 (MQ=255)
                                    tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                     >  2:3274170/1‑93 (MQ=255)
                                        aaCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCaaaa                                                 >  2:3033427/1‑93 (MQ=255)
                                         aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGTATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                                <  1:2649831/93‑1 (MQ=255)
                                         aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                                >  1:2715366/1‑93 (MQ=255)
                                           gATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTg                                              >  2:304634/1‑93 (MQ=255)
                                            aTCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGc                                             <  2:4632272/93‑1 (MQ=255)
                                            aTCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGc                                             >  1:4145740/1‑93 (MQ=255)
                                             tCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGcc                                            <  1:2336670/93‑1 (MQ=255)
                                              cGTAACGTTGATCCCACATGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCa                                           <  2:832701/93‑1 (MQ=255)
                                                 aaCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCaaaa                                        >  2:170673/1‑93 (MQ=255)
                                                  aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAc                                       >  2:3294987/1‑93 (MQ=255)
                                                  aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAc                                       >  1:2191854/1‑93 (MQ=255)
                                                     ttGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                                    <  1:5986347/93‑1 (MQ=255)
                                                      tGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGt                                   <  2:5052015/93‑1 (MQ=255)
                                                       gATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTg                                  <  1:236467/93‑1 (MQ=255)
                                                       gATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTg                                  <  1:2140338/93‑1 (MQ=255)
                                                        aTCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                                 >  2:6119863/1‑93 (MQ=255)
                                                        aTCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                                 <  1:1999877/93‑1 (MQ=255)
                                                        aTCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                                 >  1:246418/1‑93 (MQ=255)
                                                        aTCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                                 >  1:4500342/1‑93 (MQ=255)
                                                        aTCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                                 >  1:3361806/1‑93 (MQ=255)
                                                          cccACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATc                               >  1:6377684/1‑93 (MQ=255)
                                                           ccACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCt                              <  2:3123306/93‑1 (MQ=255)
                                                            cacaGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                             >  1:6410691/1‑93 (MQ=255)
                                                            cacaGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                             <  2:4969651/93‑1 (MQ=255)
                                                              caGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgtg                           <  2:5754874/93‑1 (MQ=255)
                                                               aGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                          >  2:952291/1‑93 (MQ=255)
                                                               aGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                          <  1:344850/93‑1 (MQ=255)
                                                               aGGCCCCGCACCGGATTTAGGGATGTCGGGTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                          >  1:4019615/1‑93 (MQ=255)
                                                                ggCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                         <  2:2465479/93‑1 (MQ=255)
                                                                ggCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                         >  1:2534853/1‑93 (MQ=255)
                                                                ggCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                         <  1:6094016/93‑1 (MQ=255)
                                                                ggCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                         >  2:3899039/1‑93 (MQ=255)
                                                                 gCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAg                        <  1:2428989/93‑1 (MQ=255)
                                                                 gCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAg                        <  1:3323136/93‑1 (MQ=255)
                                                                   cccGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTc                      >  1:1918320/1‑93 (MQ=255)
                                                                    ccGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCa                     <  2:1974513/93‑1 (MQ=255)
                                                                       cACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtg                  <  2:5799307/93‑1 (MQ=255)
                                                                       cACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtg                  <  1:6291822/93‑1 (MQ=255)
                                                                         ccGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc                <  1:2907725/93‑1 (MQ=255)
                                                                         ccGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc                >  1:3180201/1‑93 (MQ=255)
                                                                         ccGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc                >  2:4914201/1‑93 (MQ=255)
                                                                          cGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg               >  2:2008154/1‑93 (MQ=255)
                                                                          cGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg               >  2:4185343/1‑93 (MQ=255)
                                                                              tttAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggc           >  1:1204741/1‑93 (MQ=255)
                                                                              tttAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggc           <  2:2926366/93‑1 (MQ=255)
                                                                              tttAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggc           <  2:5543879/93‑1 (MQ=255)
                                                                              tttAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAACGGTGATCTGTGCAGTCAAtggcgtggc           >  2:286795/1‑93 (MQ=255)
                                                                                tAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTggcgg         >  1:864665/1‑93 (MQ=255)
                                                                                 aGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTggcggc        <  2:6278575/93‑1 (MQ=255)
                                                                                 aGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTggcggc        >  1:4222598/1‑93 (MQ=255)
                                                                                  gggATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca       >  2:5691823/1‑93 (MQ=255)
                                                                                  gggATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca       <  2:1926072/93‑1 (MQ=255)
                                                                                  gggATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca       >  1:3800974/1‑93 (MQ=255)
                                                                                    gATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagg     <  2:5504501/93‑1 (MQ=255)
                                                                                    gATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagg     >  1:4554764/1‑93 (MQ=255)
                                                                                     aTGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcaggg    >  2:1580687/1‑93 (MQ=255)
                                                                                      tGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcagggg   <  2:3591796/93‑1 (MQ=255)
                                                                                       gTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcaggggc  <  1:3580123/93‑1 (MQ=255)
                                                                                       gTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcaggggc  >  1:5824539/1‑93 (MQ=255)
                                                                                       gTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggcaggggc  >  2:1026872/1‑93 (MQ=255)
                                                                                          |                                                                                         
TACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC  >  NC_000913/1456446‑1456625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGC  >  NC_000913/1456443‑1456629
                                                                                             |                                                                                             
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCG                                                                                               <  SRR4302534.3449731/94‑1 (MQ=60)
 CTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCTCACAGGCCCCGCACCGGATTTAGGGATGTCGG                                                                                              >  SRR4302534.5699059/1‑94 (MQ=60)
  TTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGT                                                                                             >  SRR4302534.2534184/1‑94 (MQ=60)
  TTACAGGTGCCGGGCGCGGATTTTGTGCTAGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCTGT                                                                                             <  SRR4302534.4165709/94‑1 (MQ=60)
   TACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTT                                                                                            <  SRR4302534.2763415/94‑1 (MQ=60)
   TACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTT                                                                                            >  SRR4302534.5253357/1‑94 (MQ=60)
     CAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGA                                                                                          <  SRR4302534.1525277/94‑1 (MQ=60)
     CAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGA                                                                                          <  SRR4302534.5291056/94‑1 (MQ=60)
      AGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAA                                                                                         >  SRR4302534.1047130/1‑94 (MQ=60)
      AGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGACGGTTGAA                                                                                         >  SRR4302534.386351/1‑94 (MQ=60)
       GGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAAC                                                                                        >  SRR4302534.6361361/1‑94 (MQ=60)
         TGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGT                                                                                      <  SRR4302534.3369224/94‑1 (MQ=60)
          GCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTT                                                                                     <  SRR4302534.3941439/94‑1 (MQ=60)
           CCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTT                                                                                    <  SRR4302534.705855/94‑1 (MQ=60)
            CGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTC                                                                                   <  SRR4302534.3071168/94‑1 (MQ=60)
            CGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTC                                                                                   >  SRR4302534.3439058/1‑94 (MQ=60)
             GGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCT                                                                                  >  SRR4302534.6392169/1‑94 (MQ=60)
             GGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCT                                                                                  <  SRR4302534.632631/94‑1 (MQ=60)
             GGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGCGGTGTCGGTTGAACGTTTCT                                                                                  >  SRR4302534.2748870/1‑94 (MQ=60)
               GCGCTGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTAT                                                                                <  SRR4302534.5930224/94‑1 (MQ=60)
               GCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTAT                                                                                <  SRR4302534.5143135/94‑1 (MQ=60)
               GCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTAT                                                                                <  SRR4302534.6421545/94‑1 (MQ=60)
                CGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGCTTTAGGGATGTCGGTTGAACGTTTCTATA                                                                               >  SRR4302534.1613707/1‑94 (MQ=60)
                  CGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAC                                                                             <  SRR4302534.6576689/94‑1 (MQ=60)
                  CGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAAC                                                                             >  SRR4302534.4511641/1‑94 (MQ=60)
                   GGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACC                                                                            <  SRR4302534.3664698/94‑1 (MQ=60)
                    GATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCC                                                                           >  SRR4302534.235266/1‑94 (MQ=60)
                    GATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCC                                                                           >  SRR4302534.2956383/1‑94 (MQ=60)
                     ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCA                                                                          >  SRR4302534.1765446/1‑94 (MQ=60)
                       TTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACT                                                                        <  SRR4302534.5446241/94‑1 (MQ=60)
                         TGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGG                                                                      <  SRR4302534.1121415/94‑1 (MQ=60)
                         TGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGG                                                                      >  SRR4302534.3218276/1‑94 (MQ=60)
                          GTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGT                                                                     >  SRR4302534.4046550/1‑94 (MQ=60)
                          GTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGT                                                                     <  SRR4302534.5541558/94‑1 (MQ=60)
                           TGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTA                                                                    <  SRR4302534.5424940/94‑1 (MQ=60)
                           TGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTA                                                                    <  SRR4302534.3419232/94‑1 (MQ=60)
                            GCTGGTCAGGATCTTAACGATCGTAACGTTGAGCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTAC                                                                   <  SRR4302534.3358672/94‑1 (MQ=60)
                             CTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACG                                                                  >  SRR4302534.4384737/1‑94 (MQ=60)
                             CTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACG                                                                  <  SRR4302534.5697305/94‑1 (MQ=60)
                              TGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGT                                                                 <  SRR4302534.3498629/94‑1 (MQ=60)
                               GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTTGTACGTC                                                                <  SRR4302534.5506787/94‑1 (MQ=60)
                               GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTC                                                                <  SRR4302534.6457149/94‑1 (MQ=60)
                               GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTC                                                                >  SRR4302534.721649/1‑94 (MQ=60)
                               GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTC                                                                >  SRR4302534.2914799/1‑94 (MQ=60)
                                  CAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCC                                                             >  SRR4302534.4948146/1‑94 (MQ=60)
                                   AGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCT                                                            >  SRR4302534.6600752/1‑94 (MQ=60)
                                      ATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGC                                                         >  SRR4302534.223452/1‑94 (MQ=60)
                                       TCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCA                                                        <  SRR4302534.6167459/94‑1 (MQ=60)
                                       TCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCA                                                        <  SRR4302534.2977885/94‑1 (MQ=60)
                                       TCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCA                                                        >  SRR4302534.847030/1‑94 (MQ=60)
                                         TTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAA                                                      >  SRR4302534.4652228/1‑94 (MQ=60)
                                          TAACGATCGTAACGTTGATCCCACAGGCTCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAA                                                     >  SRR4302534.6269399/1‑94 (MQ=60)
                                          TAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAA                                                     >  SRR4302534.2990975/1‑94 (MQ=60)
                                           AACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAC                                                    >  SRR4302534.2431683/1‑94 (MQ=60)
                                           AACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAC                                                    >  SRR4302534.2787035/1‑94 (MQ=60)
                                            ACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGTATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACT                                                   <  SRR4302534.2719908/94‑1 (MQ=60)
                                            ACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACT                                                   >  SRR4302534.1850411/1‑94 (MQ=60)
                                            ACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACT                                                   <  SRR4302534.1832312/94‑1 (MQ=60)
                                            ACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACT                                                   >  SRR4302534.1680862/1‑94 (MQ=60)
                                             CGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTG                                                  >  SRR4302534.5075719/1‑94 (MQ=60)
                                              GATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGC                                                 >  SRR4302534.4257636/1‑94 (MQ=60)
                                               ATCGTAACGTTAATCCCACAGGCCCCGCACCGGCTTTAGGGATGTCGGTTGAACGTTTCTATAAGCGACTGGTACGTCGCCTTGCAAAACTGCC                                                >  SRR4302534.811107/1‑94 (MQ=60)
                                                TCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCA                                               <  SRR4302534.5992420/94‑1 (MQ=60)
                                                TCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCA                                               >  SRR4302534.4507967/1‑94 (MQ=60)
                                                TCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCA                                               <  SRR4302534.2398678/94‑1 (MQ=60)
                                                    AACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAC                                           >  SRR4302534.2249807/1‑94 (MQ=60)
                                                     ACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACC                                          >  SRR4302534.1976272/1‑94 (MQ=60)
                                                      CGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCG                                         >  SRR4302534.6546076/1‑94 (MQ=60)
                                                        TTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCTAAACCGGT                                       >  SRR4302534.4120335/1‑94 (MQ=60)
                                                        TTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGT                                       <  SRR4302534.6148963/94‑1 (MQ=60)
                                                         TGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTG                                      <  SRR4302534.708451/94‑1 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGT                                     <  SRR4302534.6632399/94‑1 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGA                                     >  SRR4302534.3451850/1‑94 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGA                                     <  SRR4302534.2403152/94‑1 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGA                                     <  SRR4302534.242657/94‑1 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGA                                     >  SRR4302534.4622153/1‑94 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGA                                     >  SRR4302534.252863/1‑94 (MQ=60)
                                                          GATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGA                                     <  SRR4302534.2196769/94‑1 (MQ=60)
                                                           ATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAT                                    <  SRR4302534.3221636/94‑1 (MQ=60)
                                                           ATCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAT                                    <  SRR4302534.2052248/94‑1 (MQ=60)
                                                            TCCCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATC                                   >  SRR4302534.6549944/1‑94 (MQ=60)
                                                              CCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTG                                 >  SRR4302534.271238/1‑94 (MQ=60)
                                                              CCACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTG                                 >  SRR4302534.6583679/1‑94 (MQ=60)
                                                                ACAGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTG                               >  SRR4302534.2493620/1‑94 (MQ=60)
                                                                 CAGGCCCCGCACCGGATTTAGGGATGTCGGGTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGC                              >  SRR4302534.4127379/1‑94 (MQ=60)
                                                                  AGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCA                             <  SRR4302534.353688/94‑1 (MQ=60)
                                                                  AGGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCA                             >  SRR4302534.2602136/1‑94 (MQ=60)
                                                                   GGCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAG                            <  SRR4302534.6259732/94‑1 (MQ=60)
                                                                    GCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGT                           <  SRR4302534.3412009/94‑1 (MQ=60)
                                                                    GCCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGT                           <  SRR4302534.2493570/94‑1 (MQ=60)
                                                                     CCCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTC                          >  SRR4302534.1968466/1‑94 (MQ=60)
                                                                      CCCGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCA                         >  SRR4302534.3936163/1‑94 (MQ=60)
                                                                        cggagCGGATTTAGGGATGTCGGTTGAACGTTTCTATAGCCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAT                       <  SRR4302534.2790124/89‑1 (MQ=60)
                                                                        CGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAT                       <  SRR4302534.4269437/94‑1 (MQ=60)
                                                                        CGCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAT                       >  SRR4302534.6028364/1‑94 (MQ=60)
                                                                         GCACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATG                      >  SRR4302534.4184588/1‑94 (MQ=60)
                                                                          CACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGG                     <  SRR4302534.6462038/94‑1 (MQ=60)
                                                                          CACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGG                     <  SRR4302534.6462039/94‑1 (MQ=60)
                                                                           ACCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGC                    >  SRR4302534.3264208/1‑94 (MQ=60)
                                                                            CCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCG                   >  SRR4302534.6442758/1‑94 (MQ=60)
                                                                            CCGGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCG                   <  SRR4302534.2984050/94‑1 (MQ=60)
                                                                              GGATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG                 <  SRR4302534.1087571/94‑1 (MQ=60)
                                                                                ATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGC               >  SRR4302534.5638008/1‑94 (MQ=60)
                                                                                ATTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGC               >  SRR4302534.1236438/1‑94 (MQ=60)
                                                                                 TTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCG              <  SRR4302534.5516157/94‑1 (MQ=60)
                                                                                 TTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCG              >  SRR4302534.118594/1‑94 (MQ=60)
                                                                                 TTTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCG              <  SRR4302534.4901095/94‑1 (MQ=60)
                                                                                  TTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGG             >  SRR4302534.886507/1‑94 (MQ=60)
                                                                                  TTAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCAAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGG             >  SRR4302534.2653358/1‑94 (MQ=60)
                                                                                   TAGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGC            >  SRR4302534.4336424/1‑94 (MQ=60)
                                                                                    AGGGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA           >  SRR4302534.3903032/1‑94 (MQ=60)
                                                                                      GGATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG         >  SRR4302534.4678240/1‑94 (MQ=60)
                                                                                      GGATGGCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG         <  SRR4302534.2280508/94‑1 (MQ=60)
                                                                                       GATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG        <  SRR4302534.4357214/94‑1 (MQ=60)
                                                                                        ATGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG       <  SRR4302534.2102597/94‑1 (MQ=60)
                                                                                        ATGTCGATTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG       <  SRR4302534.974722/94‑1 (MQ=60)
                                                                                         TGTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGC      >  SRR4302534.5982634/1‑94 (MQ=60)
                                                                                          GTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCA     >  SRR4302534.4426979/1‑94 (MQ=60)
                                                                                          GTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCA     >  SRR4302534.4549298/1‑94 (MQ=60)
                                                                                          GTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCA     <  SRR4302534.3676415/94‑1 (MQ=60)
                                                                                          GTCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCA     <  SRR4302534.34086/94‑1 (MQ=60)
                                                                                           TCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCCGGGGCAG    >  SRR4302534.245590/1‑94 (MQ=60)
                                                                                           TCGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAG    >  SRR4302534.6290285/1‑94 (MQ=60)
                                                                                            CGGTTGAACGTTTCTATAACCCACTGGTACGTCGCTTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGG   <  SRR4302534.4892484/94‑1 (MQ=60)
                                                                                            CGGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGG   >  SRR4302534.773865/1‑94 (MQ=60)
                                                                                             GGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGC  <  SRR4302534.1874002/94‑1 (MQ=60)
                                                                                             GGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGC  <  SRR4302534.4993374/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGC  >  NC_000913/1456443‑1456629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑